Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0001306

Physical Location & Seq

Genomic Coordinates Forward (+)

359257 .. 363315

Gene Structure 4059 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG004200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGGCCTCAAAACCTGGTGCTAGAGCGTCCTCGAGACTGCAACCAAAAGCAGCTGGTGTTCAAACGGTAGAATCTGATGATCTCAGGCAATTGCAATTCCCCACACACATTATGAAAGCAACCAAATCGGAGCTGTTAGCTCCCGAGCAATTCCTACAAAATTCATCAAAGCCTGTTGCAGTAGTAGAAGCATCTGACAATAAAAGCCCAGAGAATTCCAATCAAACAGGATCTGCTGATTTGTTGATTACTAAATTGAATTCGATTTCGTTGTCAGCCAACCTTGATAAGGCATCCAAGGAAGTAGATTCAGCTGTCAATCAGACTAGAGGTTCACTGGAAAATTATGCTGATCAAGAAAAGAAGATATCAGAGTATGCAAGTGTCAAGGACAGCTCAGTTTCAGCCAAGGTTAGCAATGGAGCCAGCAGTATTGCCAAGACCAGCGGAAGTGCCAAGAATTTTGTTGATAGTGGAAAGAGCAGTATGTGTAGAGGAAGCACAAGCAGTGACGTCAGTGATGAGAGCACTTGTAGCAGCTTTAGTACCACCATCAGCAAGCCACACAAAGCAAATGACTTGAGATGGGAAGCCATACAAGCTGTCCGAGCAAGAGATGGGGTTCTGGGTTTAGGTCATTTCAGATTGCTAAAGAGGTTAGGCTGTGGGGATATTGGCAGTGTCTATCTGTCGGAGTTGAGTGGAACCAAATGTTATTTTGCAATGAAGGTTATGGATAAAGAATCTCTAGCAAGTCGTAAAAAGCTTCTTCGTGCTCAGACAGAAAGAGAGATACTACAATCTCTGGATCATCCATTCCTTCCAACATTATACACCCACTTTGAGACAGATAAATTTTCATGCTTGGTAATGGAGTTCTGTCCTGGAGGAGACTTGCACACGCTTCGGCAGAGGCAGCCGGGAAAGCATTTTTCTGAGCAGGCAGTAAAATTCTATGTTGCAGAGGTCCTGCTATCTCTGGAATACCTCCACATGCTTGGAATTGTCTACCGTGACCTTAAGCCAGAAAATGTCCTGGTGAGGGAAGATGGACATATAATGCTCTCTGATTTTGACCTCTCACTCCGTTGTGCTGTTAGTCCAACTCTTGTCAACTCTTCAATGGAGTTTGAGCCCTTGCGAAAAAACCCTGTTTATTGTGTTCAGCCAGCTTGCATTGAGCCGCCTTCCTGCATCCAACCATCCTGTGTAGCTCCTACAACGTGCTTCTCACCGCGTCTGTTTTCTAGCAAGTCCAAAAAGGACAAAAAACCAAAGAACGAAATTGGTAACCAAGTACGACCATTACCTGAGCTCATGGCAGAGCCAACCGATGCCCGGTCTATGTCGTTTGTTGGGACTCATGAGTATTTGGCACCTGAGATTATTAAGGGTGAAGGTCATGGAAGTGCTGTTGATTGGTGGACTTTTGGAATCTTTCTGTATGAGCTGTTGTTTGGTAAGACTCCTTTCAAGGGTTCTGGGAATCGAGCAACATTGTTCAATGTTGTGGGTCAGCCCCTGCGGTTTCCAGAATCACCAGTAGTGAGTTTTGCTGCGAGAGATCTCATCAGGGGATTGCTTGTAAAGGAACCCCAGAACAGATTGGCATACAAACGAGGGGCAACTGAGATAAAACAACACCCCTTTTTTGAAGGCGTGAATTGGGCATTGATACGTTGTGCTACCCCGCCTGAGATCCCAAAGCCTGTTGAGATTGAGCGGATGCCTGTGCCAATAATATCATCGACTGAGAAGGAGAAGGCGCCTACCATCATGAAATCAGTTCCTGATCAAAAGGGTTCTGATAATTACCTAGAGTTTGATTTCTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

611

Amino Acids

67.47

Weight (kDa)

8.4

Isoelectric Point (pI)

45.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	215 - 364	1.9e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	215 - 364	2.2e-19	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	448 - 551	1.6e-20	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	455 - 538	6.3e-06	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26610 AT5G47750 AT5G55910 AT5G55910
fragaria_vesca	FvH4_3g00260 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710
malus_domestica	MD03G1029500.v1.1 MD09G1025300.v1.1 MD10G1339900.v1.1 MD11G1032900.v1.1
prunus_persica	Prupe.4G002900_v2.0.a1 Prupe.4G002900_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1
pyrus_communis	pycom03g02440 pycom10g28980 pycom11g02860
rosa_chinensis	RchiOBHm_Chr5g0000381 RchiOBHm_Chr5g0076451
rosa_laevigata	RLG00000030861 RLG00000030862 RLG00000036620
rosa_multiflora	Rmu_co8411395.1_g000001 Rmu_sc0000369.1_g000018 Rmu_sc0001680.1_g000004 Rmu_ssc0000181.1_g000002 Rmu_ssc0000385.1_g000014
rosa_roxburghii	Rroxscaffold_1G00075770 Rroxscaffold_1G00075780
rosa_rugosa	Rorug04G0383300 Rorug04G0383300 Rorug05G0447900 Rorug05G0448000 Rorug05G0448100
rosa_samantha	Rh5AG003200 Rh5AG502800 Rh5BG004200 Rh5BG524500 Rh5CG003400 Rh5CG548100 Rh5DG003100 Rh5DG003200 Rh5DG536300 Rh7BG161800
rosa_wichuraiana	Rw5G000310 Rw5G046640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	516
AccB1I	GGYRCC	2 cut(s)	1375, 1765
AccBSI	CCGCTC	1 cut(s)	1723
AccI	GTMKAC	1 cut(s)	1008
AccII	CGCG	1 cut(s)	1237
AciI	CCGC	6 cut(s)	447, 1184, 1235, 1525, 1691, 1723
AclWI	GGATC	3 cut(s)	238, 816, 1693
AcsI	RAATTY	6 cut(s)	160, 214, 259, 460, 854, 950
AcyI	GRCGYC	2 cut(s)	513, 1766
AfaI	GTAC	2 cut(s)	547, 1299
AfiI	CCNNNNNNNGG	4 cut(s)	289, 1042, 1338, 1475
AflII	CTTAAG	1 cut(s)	1019
AgsI	TTSAA	6 cut(s)	62, 259, 1122, 1474, 1504, 1655
AjnI	CCWGG	3 cut(s)	13, 883, 1035
Alw21I	GWGCWC	3 cut(s)	530, 778, 1317
Alw26I	GTCTC	3 cut(s)	28, 839, 885
AlwI	GGATC	3 cut(s)	238, 816, 1693
AlwNI	CAGNNNCTG	3 cut(s)	233, 1522, 1790
Ama87I	CYCGRG	2 cut(s)	31, 143
AoxI	GGCC	1 cut(s)	3
ApeKI	GCWGC	4 cut(s)	50, 537, 916, 1556
ApoI	RAATTY	6 cut(s)	160, 214, 259, 460, 854, 950
AspLEI	GCGC	1 cut(s)	1768
AspS9I	GGNCC	1 cut(s)	967
AsuC2I	CCSGG	2 cut(s)	921, 1339
AsuHPI	GGTGA	4 cut(s)	1051, 1224, 1406, 1530
AvaI	CYCGRG	2 cut(s)	31, 143
AvaII	GGWCC	1 cut(s)	967
BanI	GGYRCC	2 cut(s)	1375, 1765
BanII	GRGCYC	2 cut(s)	1137, 1317
Bbv12I	GWGCWC	3 cut(s)	530, 778, 1317
BbvI	GCAGC	4 cut(s)	62, 549, 928, 1543
BccI	CCATC	6 cut(s)	560, 579, 611, 1043, 1210, 1781
BcgI	CGANNNNNNTGC	4 cut(s)	108, 142, 887, 921
BciT130I	CCWGG	3 cut(s)	15, 885, 1037
BclI	TGATCA	2 cut(s)	352, 1792
BcnI	CCSGG	2 cut(s)	921, 1339
BcoDI	GTCTC	3 cut(s)	28, 839, 885
BfaI	CTAG	5 cut(s)	21, 327, 749, 1248, 1817
BfoI	RGCGCY	1 cut(s)	1769
BfrI	CTTAAG	1 cut(s)	1019
BglII	AGATCT	1 cut(s)	1564
BisI	GCNGC	5 cut(s)	51, 538, 917, 1184, 1557
BlsI	GCNGC	5 cut(s)	52, 539, 918, 1185, 1558
Bme1390I	CCNGG	5 cut(s)	15, 885, 921, 1037, 1339
Bme18I	GGWCC	1 cut(s)	967
BmeT110I	CYCGRG	2 cut(s)	31, 143
BmgT120I	GGNCC	1 cut(s)	967
BmiI	GGNNCC	5 cut(s)	424, 706, 1377, 1593, 1767
BmrFI	CCNGG	5 cut(s)	15, 885, 921, 1037, 1339
BmsI	GCATC	5 cut(s)	200, 302, 1203, 1324, 1716
BpmI	CTGGAG	1 cut(s)	906
Bpu10I	CCTNAGC	1 cut(s)	1311
BpuEI	CTTGAG	1 cut(s)	601
BpuMI	CCSGG	2 cut(s)	921, 1339
BsaHI	GRCGYC	2 cut(s)	513, 1766
BsaJI	CCNNGG	2 cut(s)	297, 408
Bsc4I	CCNNNNNNNGG	4 cut(s)	289, 1042, 1338, 1475
Bse1I	ACTGG	2 cut(s)	342, 1541
Bse3DI	GCAATG	2 cut(s)	424, 729
BseBI	CCWGG	3 cut(s)	15, 885, 1037
BseDI	CCNNGG	2 cut(s)	297, 408
BseGI	GGATG	5 cut(s)	293, 811, 1194, 1202, 1731
BseLI	CCNNNNNNNGG	4 cut(s)	289, 1042, 1338, 1475
BseMI	GCAATG	2 cut(s)	424, 729
BseMII	CTCAG	9 cut(s)	97, 411, 791, 927, 1302, 1371, 1620, 1686, 1743
BseNI	ACTGG	2 cut(s)	342, 1541
BseRI	GAGGAG	1 cut(s)	903
BseXI	GCAGC	4 cut(s)	62, 549, 928, 1543
Bsh1236I	CGCG	1 cut(s)	1237
BshFI	GGCC	1 cut(s)	5
BshNI	GGYRCC	2 cut(s)	1375, 1765
BsiHKAI	GWGCWC	3 cut(s)	530, 778, 1317
BsiHKCI	CYCGRG	2 cut(s)	31, 143
BsiSI	CCGG	2 cut(s)	920, 1339
BslFI	GGGAC	1 cut(s)	1372
BslI	CCNNNNNNNGG	4 cut(s)	289, 1042, 1338, 1475
BsmAI	GTCTC	3 cut(s)	28, 839, 885
BsmFI	GGGAC	1 cut(s)	1372
BsnI	GGCC	1 cut(s)	5
BsoBI	CYCGRG	2 cut(s)	31, 143
Bsp1286I	GDGCHC	4 cut(s)	530, 778, 1137, 1317
Bsp143I	GATC	7 cut(s)	79, 230, 352, 808, 1564, 1698, 1792
BspACI	CCGC	6 cut(s)	447, 1184, 1235, 1525, 1691, 1723
BspANI	GGCC	1 cut(s)	5
BspCNI	CTCAG	9 cut(s)	96, 410, 790, 928, 1303, 1372, 1621, 1687, 1744
BspFNI	CGCG	1 cut(s)	1237
BspHI	TCATGA	2 cut(s)	1363, 1776
BspLI	GGNNCC	5 cut(s)	424, 706, 1377, 1593, 1767
BspPI	GGATC	3 cut(s)	238, 816, 1693
BspT107I	GGYRCC	2 cut(s)	1375, 1765
BspTI	CTTAAG	1 cut(s)	1019
BsrBI	CCGCTC	1 cut(s)	1723
BsrDI	GCAATG	2 cut(s)	424, 729
BsrI	ACTGG	2 cut(s)	342, 1541
BssECI	CCNNGG	2 cut(s)	297, 408
BssMI	GATC	7 cut(s)	79, 230, 352, 808, 1564, 1698, 1792
BssNI	GRCGYC	2 cut(s)	513, 1766
BssT1I	CCWWGG	2 cut(s)	297, 408
Bst2UI	CCWGG	3 cut(s)	15, 885, 1037
Bst4CI	ACNGT	2 cut(s)	67, 1013
Bst6I	CTCTTC	1 cut(s)	1123
BstACI	GRCGYC	2 cut(s)	513, 1766
BstAFI	CTTAAG	1 cut(s)	1019
BstC8I	GCNNGC	5 cut(s)	427, 560, 942, 1170, 1174
BstDEI	CTNAG	9 cut(s)	83, 397, 777, 936, 1311, 1380, 1629, 1695, 1752
BstEII	GGTNACC	1 cut(s)	1289
BstENI	CCTNNNNNAGG	1 cut(s)	287
BstF5I	GGATG	5 cut(s)	293, 811, 1194, 1202, 1731
BstFNI	CGCG	1 cut(s)	1237
BstH2I	RGCGCY	1 cut(s)	1769
BstHHI	GCGC	1 cut(s)	1768
BstKTI	GATC	7 cut(s)	82, 233, 355, 811, 1567, 1701, 1795
BstMAI	GTCTC	3 cut(s)	28, 839, 885
BstMBI	GATC	7 cut(s)	79, 230, 352, 808, 1564, 1698, 1792
BstMWI	GCNNNNNNNGC	5 cut(s)	534, 599, 608, 916, 925
BstNI	CCWGG	3 cut(s)	15, 885, 1037
BstNSI	RCATGY	1 cut(s)	997
BstPI	GGTNACC	1 cut(s)	1289
BstSCI	CCNGG	5 cut(s)	13, 883, 919, 1035, 1337
BstUI	CGCG	1 cut(s)	1237
BstV1I	GCAGC	4 cut(s)	62, 549, 928, 1543
BstX2I	RGATCY	3 cut(s)	230, 1564, 1698
BstXI	CCANNNNNNTGG	1 cut(s)	998
BstYI	RGATCY	3 cut(s)	230, 1564, 1698
BsuRI	GGCC	1 cut(s)	5
BtsCI	GGATG	5 cut(s)	293, 811, 1194, 1202, 1731
BtsI	GCAGTG	2 cut(s)	514, 685
BtsIMutI	CAGTG	4 cut(s)	335, 514, 523, 685
Cac8I	GCNNGC	5 cut(s)	427, 560, 942, 1170, 1174
CaiI	CAGNNNCTG	3 cut(s)	233, 1522, 1790
CciI	TCATGA	2 cut(s)	1363, 1776
CfoI	GCGC	1 cut(s)	1768
Cfr13I	GGNCC	1 cut(s)	967
CseI	GACGC	2 cut(s)	15, 1226
CsiI	ACCWGGT	1 cut(s)	13
Csp6I	GTAC	2 cut(s)	546, 1298
CviAII	CATG	6 cut(s)	861, 994, 1318, 1364, 1403, 1777
CviQI	GTAC	2 cut(s)	546, 1298
DdeI	CTNAG	9 cut(s)	83, 397, 777, 936, 1311, 1380, 1629, 1695, 1752
DinI	GGCGCC	1 cut(s)	1767
DpnI	GATC	7 cut(s)	81, 232, 354, 810, 1566, 1700, 1794
DpnII	GATC	7 cut(s)	79, 230, 352, 808, 1564, 1698, 1792
Eam1104I	CTCTTC	1 cut(s)	1123
EarI	CTCTTC	1 cut(s)	1123
Ecl136II	GAGCTC	1 cut(s)	1315
Eco130I	CCWWGG	2 cut(s)	297, 408
Eco24I	GRGCYC	2 cut(s)	1137, 1317
Eco32I	GATATC	1 cut(s)	369
Eco47I	GGWCC	1 cut(s)	967
Eco53kI	GAGCTC	1 cut(s)	1315
Eco88I	CYCGRG	2 cut(s)	31, 143
Eco91I	GGTNACC	1 cut(s)	1289
EcoICRI	GAGCTC	1 cut(s)	1315
EcoNI	CCTNNNNNAGG	1 cut(s)	287
EcoO109I	RGGNCCY	1 cut(s)	967
EcoO65I	GGTNACC	1 cut(s)	1289
EcoRI	GAATTC	2 cut(s)	214, 259
EcoRII	CCWGG	3 cut(s)	13, 883, 1035
EcoRV	GATATC	1 cut(s)	369
EcoT14I	CCWWGG	2 cut(s)	297, 408
EcoT38I	GRGCYC	2 cut(s)	1137, 1317
EgeI	GGCGCC	1 cut(s)	1767
EheI	GGCGCC	1 cut(s)	1767
ErhI	CCWWGG	2 cut(s)	297, 408
FaeI	CATG	6 cut(s)	864, 997, 1321, 1367, 1406, 1780
FaqI	GGGAC	1 cut(s)	1372
FatI	CATG	6 cut(s)	860, 993, 1317, 1363, 1402, 1776
FauI	CCCGC	1 cut(s)	1698
FbaI	TGATCA	2 cut(s)	352, 1792
FblI	GTMKAC	1 cut(s)	1008
Fnu4HI	GCNGC	5 cut(s)	51, 538, 917, 1184, 1557
FokI	GGATG	5 cut(s)	280, 798, 1181, 1189, 1738
FriOI	GRGCYC	2 cut(s)	1137, 1317
Fsp4HI	GCNGC	5 cut(s)	51, 538, 917, 1184, 1557
FspBI	CTAG	5 cut(s)	21, 327, 749, 1248, 1817
GlaI	GCGC	1 cut(s)	1767
GluI	GCNGC	5 cut(s)	51, 538, 917, 1184, 1557
GsuI	CTGGAG	1 cut(s)	906
HaeII	RGCGCY	1 cut(s)	1769
HaeIII	GGCC	1 cut(s)	5
HapII	CCGG	2 cut(s)	920, 1339
HgaI	GACGC	2 cut(s)	15, 1226
HhaI	GCGC	1 cut(s)	1768
Hin1I	GRCGYC	2 cut(s)	513, 1766
Hin1II	CATG	6 cut(s)	864, 997, 1321, 1367, 1406, 1780
Hin6I	GCGC	1 cut(s)	1766
HinP1I	GCGC	1 cut(s)	1766
HincII	GTYRAC	1 cut(s)	1114
HindII	GTYRAC	1 cut(s)	1114
HindIII	AAGCTT	1 cut(s)	764
HinfI	GANTC	8 cut(s)	71, 308, 743, 1360, 1434, 1465, 1486, 1535
HpaII	CCGG	2 cut(s)	920, 1339
HphI	GGTGA	4 cut(s)	1051, 1224, 1406, 1530
Hpy166II	GTNNAC	4 cut(s)	335, 1009, 1114, 1425
Hpy188III	TCNNGA	9 cut(s)	33, 143, 356, 806, 980, 1364, 1532, 1777, 1790
Hpy8I	GTNNAC	4 cut(s)	335, 1009, 1114, 1425
HpyAV	CCTTC	7 cut(s)	721, 830, 1197, 1391, 1649, 1750, 1756
HpyCH4III	ACNGT	2 cut(s)	67, 1013
HpyCH4IV	ACGT	3 cut(s)	513, 1223, 1678
HpyCH4V	TGCA	9 cut(s)	40, 94, 179, 380, 722, 898, 962, 1176, 1194
HpyF10VI	GCNNNNNNNGC	5 cut(s)	534, 599, 608, 916, 925
HpyF3I	CTNAG	9 cut(s)	83, 397, 777, 936, 1311, 1380, 1629, 1695, 1752
HpySE526I	ACGT	3 cut(s)	513, 1223, 1678
Hsp92I	GRCGYC	2 cut(s)	513, 1766
Hsp92II	CATG	6 cut(s)	864, 997, 1321, 1367, 1406, 1780
HspAI	GCGC	1 cut(s)	1766
KasI	GGCGCC	1 cut(s)	1765
Ksp22I	TGATCA	2 cut(s)	352, 1792
Kzo9I	GATC	7 cut(s)	79, 230, 352, 808, 1564, 1698, 1792
LmnI	GCTCC	4 cut(s)	130, 145, 422, 1219
Lsp1109I	GCAGC	4 cut(s)	62, 549, 928, 1543
LweI	GCATC	5 cut(s)	200, 302, 1203, 1324, 1716
MabI	ACCWGGT	1 cut(s)	13
MaeI	CTAG	5 cut(s)	21, 327, 749, 1248, 1817
MaeII	ACGT	3 cut(s)	513, 1223, 1678
MaeIII	GTNAC	3 cut(s)	509, 1013, 1289
MalI	GATC	7 cut(s)	81, 232, 354, 810, 1566, 1700, 1794
MbiI	CCGCTC	1 cut(s)	1723
MboI	GATC	7 cut(s)	79, 230, 352, 808, 1564, 1698, 1792
MboII	GAAGA	4 cut(s)	376, 761, 1058, 1110
MfeI	CAATTG	1 cut(s)	89
MflI	RGATCY	3 cut(s)	230, 1564, 1698
MhlI	GDGCHC	4 cut(s)	530, 778, 1137, 1317
Mly113I	GGCGCC	1 cut(s)	1766
MlyI	GAGTC	2 cut(s)	1354, 1459
MmeI	TCCRAC	4 cut(s)	672, 848, 1127, 1222
MseI	TTAA	2 cut(s)	1020, 1389
MslI	CAYNNNNRTG	1 cut(s)	1407
MspA1I	CMGCKG	3 cut(s)	53, 314, 447
MspCI	CTTAAG	1 cut(s)	1019
MspI	CCGG	2 cut(s)	920, 1339
MspR9I	CCNGG	5 cut(s)	15, 885, 921, 1037, 1339
MunI	CAATTG	1 cut(s)	89
MvaI	CCWGG	3 cut(s)	15, 885, 1037
MvnI	CGCG	1 cut(s)	1237
MwoI	GCNNNNNNNGC	5 cut(s)	534, 599, 608, 916, 925
NarI	GGCGCC	1 cut(s)	1766
NciI	CCSGG	2 cut(s)	921, 1339
NdeII	GATC	7 cut(s)	79, 230, 352, 808, 1564, 1698, 1792
NlaIII	CATG	6 cut(s)	864, 997, 1321, 1367, 1406, 1780
NlaIV	GGNNCC	5 cut(s)	424, 706, 1377, 1593, 1767
NmuCI	GTSAC	2 cut(s)	509, 1013
NspI	RCATGY	1 cut(s)	997
PaeR7I	CTCGAG	1 cut(s)	31
PagI	TCATGA	2 cut(s)	1363, 1776
PfeI	GAWTC	6 cut(s)	71, 308, 743, 1434, 1486, 1535
PfoI	TCCNGGA	1 cut(s)	883
PkrI	GCNGC	5 cut(s)	52, 539, 918, 1185, 1558
PleI	GAGTC	2 cut(s)	1354, 1459
PluTI	GGCGCC	1 cut(s)	1769
PpsI	GAGTC	2 cut(s)	1354, 1459
PpuMI	RGGWCCY	1 cut(s)	967
Psp124BI	GAGCTC	1 cut(s)	1317
Psp5II	RGGWCCY	1 cut(s)	967
Psp6I	CCWGG	3 cut(s)	13, 883, 1035
PspEI	GGTNACC	1 cut(s)	1289
PspGI	CCWGG	3 cut(s)	13, 883, 1035
PspN4I	GGNNCC	5 cut(s)	424, 706, 1377, 1593, 1767
PspPI	GGNCC	1 cut(s)	967
PspPPI	RGGWCCY	1 cut(s)	967
PstNI	CAGNNNCTG	3 cut(s)	233, 1522, 1790
PsuI	RGATCY	3 cut(s)	230, 1564, 1698
PvuII	CAGCTG	2 cut(s)	53, 314
RsaI	GTAC	2 cut(s)	547, 1299
RsaNI	GTAC	2 cut(s)	546, 1298
RseI	CAYNNNNRTG	1 cut(s)	1407
SacI	GAGCTC	1 cut(s)	1317
SaqAI	TTAA	2 cut(s)	1020, 1389
SatI	GCNGC	5 cut(s)	51, 538, 917, 1184, 1557
Sau3AI	GATC	7 cut(s)	79, 230, 352, 808, 1564, 1698, 1792
Sau96I	GGNCC	1 cut(s)	967
SchI	GAGTC	2 cut(s)	1354, 1459
ScrFI	CCNGG	5 cut(s)	15, 885, 921, 1037, 1339
SduI	GDGCHC	4 cut(s)	530, 778, 1137, 1317
SexAI	ACCWGGT	1 cut(s)	13
SfaNI	GCATC	5 cut(s)	200, 302, 1203, 1324, 1716
SfoI	GGCGCC	1 cut(s)	1767
Sfr274I	CTCGAG	1 cut(s)	31
SinI	GGWCC	1 cut(s)	967
SlaI	CTCGAG	1 cut(s)	31
SmiMI	CAYNNNNRTG	1 cut(s)	1407
SmlI	CTYRAG	3 cut(s)	31, 580, 1019
SmoI	CTYRAG	3 cut(s)	31, 580, 1019
SsiI	CCGC	6 cut(s)	447, 1184, 1235, 1525, 1691, 1723
SspDI	GGCGCC	1 cut(s)	1765
SspMI	CTAG	5 cut(s)	21, 327, 749, 1248, 1817
SstI	GAGCTC	1 cut(s)	1317
StyD4I	CCNGG	5 cut(s)	13, 883, 919, 1035, 1337
StyI	CCWWGG	2 cut(s)	297, 408
TaaI	ACNGT	2 cut(s)	67, 1013
TaiI	ACGT	3 cut(s)	516, 1226, 1681
TaqI	TCGA	4 cut(s)	32, 263, 1489, 1748
TauI	GCSGC	1 cut(s)	1186
TfiI	GAWTC	6 cut(s)	71, 308, 743, 1434, 1486, 1535
Tru1I	TTAA	2 cut(s)	1020, 1389
Tru9I	TTAA	2 cut(s)	1020, 1389
TscAI	CASTG	4 cut(s)	342, 514, 523, 685
TseFI	GTSAC	2 cut(s)	509, 1013
TseI	GCWGC	4 cut(s)	50, 537, 916, 1556
Tsp45I	GTSAC	2 cut(s)	509, 1013
TspDTI	ATGAA	5 cut(s)	128, 153, 740, 849, 1793
TspGWI	ACGGA	1 cut(s)	1076
TspRI	CASTG	4 cut(s)	342, 514, 523, 685
Vha464I	CTTAAG	1 cut(s)	1019
VpaK11BI	GGWCC	1 cut(s)	967
XagI	CCTNNNNNAGG	1 cut(s)	287
XapI	RAATTY	6 cut(s)	160, 214, 259, 460, 854, 950
XceI	RCATGY	1 cut(s)	997
XhoI	CTCGAG	1 cut(s)	31
XmiI	GTMKAC	1 cut(s)	1008
XspI	CTAG	5 cut(s)	21, 327, 749, 1248, 1817
ZraI	GACGTC	1 cut(s)	514

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.