Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0001306

Physical Location & Seq

Genomic Coordinates Forward (+)

271726 .. 274529

Gene Structure 2804 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030861

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGCCACAAAATCTGGTGCCACCACAGCTTCCTCGAGACTGCAACCGAAACCAGCCGGTGCTCAAACGGTAGAATCAGATGACCTCAGGCAATTGCCTACACACAATATCAAGGTAACCAAATCAGAGCTGGCGGCTCCTGAGCAATTCCTGAAATCTGTACAAAATTCATCAAAGCATGTTGCAGTAGCAGCTTCTGATAATAATAATAATAAAAGGCCAGAAAATTCCAATCAAACAGGATCTGCTGATTTGTTGGTTACTAAATTGAATTCGATTTCATTGTCACCCAACCTTGAGAATGCATCAAAGGAAGTGGATTCTGCTGTAAATCAGACAGCGGAAAGTTTTGTTGATCAAGAAAAGAAGATATCAGAGTATGAAAGTCTCAAGGACAGCTCAGTTTCGGCCAAAGTTAGCGATGGAGCCAGCAGTATTGCCAAGACTAGTGGAAGTGCCAAGAATTTTGTTGAAAATAGCAAGAGCAGCATGTGTAGAGGAAGCACAAGTAGTGATGTCAGTGATGAGAGCACTTGTAGCAGCTTTAGTACCAGCATCAGCAAGCCACACAAAGCAAATGACATGAGATGGGAAGCTATCCAAGCTGTCCGAGCAAGAGATGGGGTTCTGGGTTTAGGTCATTTCAGATTGCTAAAGAGGTTGGGCTGTGGGGATATTGGGAGTGTCTATCTGTCGGAGTTGAGCGGAACCAAATGTTATTTTGCAATGAAGGTTATGGACAAAGGGTCTCTAGCAAGTCGTAAAAAGCTTCTTCGTGCTCAGACAGAGAGAGAGATACTACAGTCTCTGGATCATCCCTTCCTTCCAACATTATACACCCACTTTGAGACAGATAAATTTTCATGCTTGGTAATGGAGTTCTGTCCTGGAGGAGACTTGCACACTCTTCGGCAGAGGCAGCCAGGAAAGCATTTTTCTGAGCAGGCAGTAAAATTCTATGTTGCAGAGGTCCTGTTATCTCTGGAATACCTCCACATGCTTGGGATTGTCTATCGTGACCTTAAGCCAGAAAATGTCCTGGTGAGGGAAGATGGACATATAATGCTCTCTGATTTTGATCTCTCACTCCGTTGTGCTTTTAGTCCAACTCTTGTCAAGACCTCTTCAATGGAGTTTGAGCCCTTGCGTAAAAACCCTGTTTATTGTGTTCAACCAGCTTGCATTGAGCCGCCTTCCTGCATCCAACCATCCTGTGTAGCTCCTACAACGTGCTTCTCACCGCGTCTATTTTCTAGCAAGTCCAAAAAGGATAAAAAACCTAAAAACGAAACTGGTAACCAAGTAAGACCATTACCTGAGCTCATGGCAGAACCAACTGATGCCCGGTCTATGTCATTTGTTGGTACTCATGAGTATTTGGCACCTGAGATTATTAAGGGTGAAGGTCATGGAAGTGCTGTTGATTGGTGGACTTTTGGAATCTTTTTGTATGAGCTGTTGTTTGGTAAGACTCCTTTCAAGGGTTCTGGGAATCGAGCAACATTGTTCAATGTTGTGGGTCAGCCCTTGCGGTTTCCAGAATCACCAGTTGTGAGTTTTGCTGCGAGAGACCTCATCAGGGGATTGCTAGTCAAGGAACCGCAGAACAGGTTGGCATACAAACGAGGGGCAACTGAGATAAAGCAACACCCCTTTTTTGAAGGAGTGAATTGGGCATTGATACGTTGTGCAACCCCGCCTGAGATCCCAAAGCCTGTTGAGATTGAGCAGATACCTGTCCCGACAATGTCATCGAGTGAGAAGGAGAAGGCGCCTACCATGATGAAAGCAGCTCCTGATCAAAAGGGTTCCGATAATTACCTAGAATTTGATTTCTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

614

Amino Acids

67.62

Weight (kDa)

8.27

Isoelectric Point (pI)

43.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	216 - 365	8.3e-36	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	216 - 365	4e-19	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	450 - 553	1.6e-20	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	457 - 540	6.4e-06	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26610 AT5G47750 AT5G55910 AT5G55910
fragaria_vesca	FvH4_3g00260 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710
malus_domestica	MD03G1029500.v1.1 MD09G1025300.v1.1 MD10G1339900.v1.1 MD11G1032900.v1.1
prunus_persica	Prupe.4G002900_v2.0.a1 Prupe.4G002900_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1
pyrus_communis	pycom03g02440 pycom10g28980 pycom11g02860
rosa_chinensis	RchiOBHm_Chr5g0000381 RchiOBHm_Chr5g0076451
rosa_laevigata	RLG00000030861 RLG00000030862 RLG00000036620
rosa_multiflora	Rmu_co8411395.1_g000001 Rmu_sc0000369.1_g000018 Rmu_sc0001680.1_g000004 Rmu_ssc0000181.1_g000002 Rmu_ssc0000385.1_g000014
rosa_roxburghii	Rroxscaffold_1G00075770 Rroxscaffold_1G00075780
rosa_rugosa	Rorug04G0383300 Rorug04G0383300 Rorug05G0447900 Rorug05G0448000 Rorug05G0448100
rosa_samantha	Rh5AG003200 Rh5AG502800 Rh5BG004200 Rh5BG524500 Rh5CG003400 Rh5CG548100 Rh5DG003100 Rh5DG003200 Rh5DG536300 Rh7BG161800
rosa_wichuraiana	Rw5G000310 Rw5G046640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	17, 1381, 1771
AccBSI	CCGCTC	1 cut(s)	705
AccII	CGCG	1 cut(s)	1243
AciI	CCGC	8 cut(s)	134, 341, 705, 1190, 1241, 1531, 1601, 1697
AclWI	GGATC	3 cut(s)	250, 819, 1699
AcoI	YGGCCR	2 cut(s)	3, 408
AcsI	RAATTY	7 cut(s)	166, 226, 271, 463, 857, 953, 1826
AcyI	GRCGYC	1 cut(s)	1772
AfaI	GTAC	3 cut(s)	162, 550, 1366
AfiI	CCNNNNNNNGG	3 cut(s)	1045, 1481, 1579
AflII	CTTAAG	1 cut(s)	1022
AgsI	TTSAA	7 cut(s)	271, 473, 1128, 1172, 1480, 1510, 1661
AhdI	GACNNNNNGTC	1 cut(s)	745
AhlI	ACTAGT	1 cut(s)	446
AjnI	CCWGG	3 cut(s)	886, 922, 1038
Alw21I	GWGCWC	4 cut(s)	64, 533, 781, 1323
Alw26I	GTCTC	7 cut(s)	31, 392, 753, 810, 842, 888, 1563
AlwI	GGATC	3 cut(s)	250, 819, 1699
AlwNI	CAGNNNCTG	4 cut(s)	197, 245, 808, 1796
Ama87I	CYCGRG	1 cut(s)	34
AoxI	GGCC	3 cut(s)	3, 218, 408
ApeKI	GCWGC	6 cut(s)	191, 486, 540, 919, 1562, 1790
ApoI	RAATTY	7 cut(s)	166, 226, 271, 463, 857, 953, 1826
AspLEI	GCGC	1 cut(s)	1774
AspS9I	GGNCC	1 cut(s)	970
AsuC2I	CCSGG	1 cut(s)	1345
AsuHPI	GGTGA	5 cut(s)	279, 1054, 1230, 1412, 1536
AvaI	CYCGRG	1 cut(s)	34
AvaII	GGWCC	1 cut(s)	970
AxyI	CCTNAGG	1 cut(s)	86
BaeI	ACNNNNGTAYC	2 cut(s)	788, 821
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	3 cut(s)	17, 1381, 1771
BanII	GRGCYC	2 cut(s)	1143, 1323
Bbv12I	GWGCWC	4 cut(s)	64, 533, 781, 1323
BbvI	GCAGC	6 cut(s)	203, 498, 552, 931, 1549, 1802
BccI	CCATC	5 cut(s)	416, 582, 614, 1046, 1216
BcgI	CGANNNNNNTGC	2 cut(s)	890, 924
BciT130I	CCWGG	3 cut(s)	888, 924, 1040
BclI	TGATCA	2 cut(s)	355, 1798
BcnI	CCSGG	1 cut(s)	1345
BcoDI	GTCTC	7 cut(s)	31, 392, 753, 810, 842, 888, 1563
BcuI	ACTAGT	1 cut(s)	446
BfaI	CTAG	5 cut(s)	447, 752, 1254, 1589, 1823
BfmI	CTRYAG	1 cut(s)	800
BfoI	RGCGCY	1 cut(s)	1775
BfrI	CTTAAG	1 cut(s)	1022
BisI	GCNGC	8 cut(s)	135, 192, 487, 541, 920, 1190, 1563, 1791
BlsI	GCNGC	8 cut(s)	136, 193, 488, 542, 921, 1191, 1564, 1792
Bme1390I	CCNGG	4 cut(s)	888, 924, 1040, 1345
Bme18I	GGWCC	1 cut(s)	970
BmeRI	GACNNNNNGTC	1 cut(s)	745
BmeT110I	CYCGRG	1 cut(s)	34
BmgT120I	GGNCC	1 cut(s)	970
BmiI	GGNNCC	8 cut(s)	19, 138, 427, 709, 1383, 1599, 1773, 1810
BmrFI	CCNGG	4 cut(s)	888, 924, 1040, 1345
BmsI	GCATC	4 cut(s)	314, 564, 1209, 1330
BpmI	CTGGAG	1 cut(s)	909
Bpu10I	CCTNAGC	2 cut(s)	141, 1317
BpuEI	CTTGAG	2 cut(s)	317, 374
BpuMI	CCSGG	1 cut(s)	1345
BsaBI	GATNNNNATC	1 cut(s)	1077
BsaHI	GRCGYC	1 cut(s)	1772
BsaI	GGTCTC	2 cut(s)	753, 1563
Bsc4I	CCNNNNNNNGG	3 cut(s)	1045, 1481, 1579
Bse118I	RCCGGY	1 cut(s)	56
Bse1I	ACTGG	2 cut(s)	1297, 1547
Bse21I	CCTNAGG	1 cut(s)	86
Bse3DI	GCAATG	1 cut(s)	732
Bse8I	GATNNNNATC	1 cut(s)	1077
BseBI	CCWGG	3 cut(s)	888, 924, 1040
BseGI	GGATG	3 cut(s)	814, 1200, 1208
BseJI	GATNNNNATC	1 cut(s)	1077
BseLI	CCNNNNNNNGG	3 cut(s)	1045, 1481, 1579
BseMI	GCAATG	1 cut(s)	732
BseMII	CTCAG	9 cut(s)	100, 132, 414, 794, 930, 1308, 1377, 1626, 1692
BseNI	ACTGG	2 cut(s)	1297, 1547
BseRI	GAGGAG	1 cut(s)	906
BseXI	GCAGC	6 cut(s)	203, 498, 552, 931, 1549, 1802
Bsh1236I	CGCG	1 cut(s)	1243
BshFI	GGCC	3 cut(s)	5, 220, 410
BshNI	GGYRCC	3 cut(s)	17, 1381, 1771
BsiHKAI	GWGCWC	4 cut(s)	64, 533, 781, 1323
BsiHKCI	CYCGRG	1 cut(s)	34
BsiSI	CCGG	2 cut(s)	57, 1345
BslFI	GGGAC	1 cut(s)	1724
BslI	CCNNNNNNNGG	3 cut(s)	1045, 1481, 1579
BsmAI	GTCTC	7 cut(s)	31, 392, 753, 810, 842, 888, 1563
BsmFI	GGGAC	1 cut(s)	1724
BsmI	GAATGC	1 cut(s)	307
BsnI	GGCC	3 cut(s)	5, 220, 410
Bso31I	GGTCTC	2 cut(s)	753, 1563
BsoBI	CYCGRG	1 cut(s)	34
Bsp1286I	GDGCHC	5 cut(s)	64, 533, 781, 1143, 1323
Bsp1407I	TGTACA	1 cut(s)	160
Bsp143I	GATC	6 cut(s)	242, 355, 811, 1078, 1704, 1798
BspACI	CCGC	8 cut(s)	134, 341, 705, 1190, 1241, 1531, 1601, 1697
BspANI	GGCC	3 cut(s)	5, 220, 410
BspCNI	CTCAG	9 cut(s)	99, 133, 413, 793, 931, 1309, 1378, 1627, 1693
BspFNI	CGCG	1 cut(s)	1243
BspHI	TCATGA	1 cut(s)	1369
BspLI	GGNNCC	8 cut(s)	19, 138, 427, 709, 1383, 1599, 1773, 1810
BspPI	GGATC	3 cut(s)	250, 819, 1699
BspT107I	GGYRCC	3 cut(s)	17, 1381, 1771
BspTI	CTTAAG	1 cut(s)	1022
BspTNI	GGTCTC	2 cut(s)	753, 1563
BsrBI	CCGCTC	1 cut(s)	705
BsrDI	GCAATG	1 cut(s)	732
BsrFI	RCCGGY	1 cut(s)	56
BsrGI	TGTACA	1 cut(s)	160
BsrI	ACTGG	2 cut(s)	1297, 1547
BssAI	RCCGGY	1 cut(s)	56
BssMI	GATC	6 cut(s)	242, 355, 811, 1078, 1704, 1798
BssNI	GRCGYC	1 cut(s)	1772
Bst2UI	CCWGG	3 cut(s)	888, 924, 1040
Bst4CI	ACNGT	2 cut(s)	70, 804
Bst6I	CTCTTC	2 cut(s)	912, 1129
BstACI	GRCGYC	1 cut(s)	1772
BstAFI	CTTAAG	1 cut(s)	1022
BstAUI	TGTACA	1 cut(s)	160
BstC8I	GCNNGC	5 cut(s)	132, 430, 563, 945, 1180
BstDEI	CTNAG	9 cut(s)	86, 141, 400, 780, 939, 1317, 1386, 1635, 1701
BstEII	GGTNACC	2 cut(s)	115, 1295
BstF5I	GGATG	3 cut(s)	814, 1200, 1208
BstFNI	CGCG	1 cut(s)	1243
BstH2I	RGCGCY	1 cut(s)	1775
BstHHI	GCGC	1 cut(s)	1774
BstKTI	GATC	6 cut(s)	245, 358, 814, 1081, 1707, 1801
BstMAI	GTCTC	7 cut(s)	31, 392, 753, 810, 842, 888, 1563
BstMBI	GATC	6 cut(s)	242, 355, 811, 1078, 1704, 1798
BstMWI	GCNNNNNNNGC	8 cut(s)	26, 191, 486, 537, 602, 611, 919, 928
BstNI	CCWGG	3 cut(s)	888, 924, 1040
BstNSI	RCATGY	3 cut(s)	182, 493, 1000
BstPI	GGTNACC	2 cut(s)	115, 1295
BstSCI	CCNGG	4 cut(s)	886, 922, 1038, 1343
BstSFI	CTRYAG	1 cut(s)	800
BstUI	CGCG	1 cut(s)	1243
BstV1I	GCAGC	6 cut(s)	203, 498, 552, 931, 1549, 1802
BstX2I	RGATCY	2 cut(s)	242, 1704
BstXI	CCANNNNNNTGG	1 cut(s)	1001
BstYI	RGATCY	2 cut(s)	242, 1704
Bsu36I	CCTNAGG	1 cut(s)	86
BsuRI	GGCC	3 cut(s)	5, 220, 410
BtgZI	GCGATG	1 cut(s)	435
BtsCI	GGATG	3 cut(s)	814, 1200, 1208
BtsIMutI	CAGTG	1 cut(s)	526
Cac8I	GCNNGC	5 cut(s)	132, 430, 563, 945, 1180
CaiI	CAGNNNCTG	4 cut(s)	197, 245, 808, 1796
CciI	TCATGA	1 cut(s)	1369
CfoI	GCGC	1 cut(s)	1774
Cfr10I	RCCGGY	1 cut(s)	56
Cfr13I	GGNCC	1 cut(s)	970
CseI	GACGC	1 cut(s)	1232
Csp6I	GTAC	3 cut(s)	161, 549, 1365
CspCI	CAANNNNNGTGG	4 cut(s)	297, 332, 430, 465
CviAII	CATG	9 cut(s)	179, 490, 583, 864, 997, 1324, 1370, 1409, 1780
CviQI	GTAC	3 cut(s)	161, 549, 1365
DdeI	CTNAG	9 cut(s)	86, 141, 400, 780, 939, 1317, 1386, 1635, 1701
DinI	GGCGCC	1 cut(s)	1773
DpnI	GATC	6 cut(s)	244, 357, 813, 1080, 1706, 1800
DpnII	GATC	6 cut(s)	242, 355, 811, 1078, 1704, 1798
DriI	GACNNNNNGTC	1 cut(s)	745
EaeI	YGGCCR	2 cut(s)	3, 408
Eam1104I	CTCTTC	2 cut(s)	912, 1129
Eam1105I	GACNNNNNGTC	1 cut(s)	745
EarI	CTCTTC	2 cut(s)	912, 1129
Ecl136II	GAGCTC	1 cut(s)	1321
Eco24I	GRGCYC	2 cut(s)	1143, 1323
Eco31I	GGTCTC	2 cut(s)	753, 1563
Eco32I	GATATC	1 cut(s)	372
Eco47I	GGWCC	1 cut(s)	970
Eco53kI	GAGCTC	1 cut(s)	1321
Eco81I	CCTNAGG	1 cut(s)	86
Eco88I	CYCGRG	1 cut(s)	34
Eco91I	GGTNACC	2 cut(s)	115, 1295
EcoICRI	GAGCTC	1 cut(s)	1321
EcoO109I	RGGNCCY	1 cut(s)	970
EcoO65I	GGTNACC	2 cut(s)	115, 1295
EcoRI	GAATTC	1 cut(s)	271
EcoRII	CCWGG	3 cut(s)	886, 922, 1038
EcoRV	GATATC	1 cut(s)	372
EcoT22I	ATGCAT	1 cut(s)	307
EcoT38I	GRGCYC	2 cut(s)	1143, 1323
EgeI	GGCGCC	1 cut(s)	1773
EheI	GGCGCC	1 cut(s)	1773
FaeI	CATG	9 cut(s)	182, 493, 586, 867, 1000, 1327, 1373, 1412, 1783
FaqI	GGGAC	1 cut(s)	1724
FatI	CATG	9 cut(s)	178, 489, 582, 863, 996, 1323, 1369, 1408, 1779
FauI	CCCGC	1 cut(s)	1704
FbaI	TGATCA	2 cut(s)	355, 1798
Fnu4HI	GCNGC	8 cut(s)	135, 192, 487, 541, 920, 1190, 1563, 1791
FokI	GGATG	3 cut(s)	801, 1187, 1195
FriOI	GRGCYC	2 cut(s)	1143, 1323
Fsp4HI	GCNGC	8 cut(s)	135, 192, 487, 541, 920, 1190, 1563, 1791
FspBI	CTAG	5 cut(s)	447, 752, 1254, 1589, 1823
GlaI	GCGC	1 cut(s)	1773
GluI	GCNGC	8 cut(s)	135, 192, 487, 541, 920, 1190, 1563, 1791
GsuI	CTGGAG	1 cut(s)	909
HaeII	RGCGCY	1 cut(s)	1775
HaeIII	GGCC	3 cut(s)	5, 220, 410
HapII	CCGG	2 cut(s)	57, 1345
HgaI	GACGC	1 cut(s)	1232
HhaI	GCGC	1 cut(s)	1774
Hin1I	GRCGYC	1 cut(s)	1772
Hin1II	CATG	9 cut(s)	182, 493, 586, 867, 1000, 1327, 1373, 1412, 1783
Hin6I	GCGC	1 cut(s)	1772
HinP1I	GCGC	1 cut(s)	1772
HindIII	AAGCTT	1 cut(s)	767
HinfI	GANTC	6 cut(s)	74, 320, 1440, 1471, 1492, 1541
HpaII	CCGG	2 cut(s)	57, 1345
HphI	GGTGA	5 cut(s)	279, 1054, 1230, 1412, 1536
Hpy166II	GTNNAC	1 cut(s)	1431
Hpy8I	GTNNAC	1 cut(s)	1431
HpyAV	CCTTC	8 cut(s)	724, 829, 833, 1203, 1397, 1655, 1756, 1762
HpyCH4III	ACNGT	2 cut(s)	70, 804
HpyCH4IV	ACGT	2 cut(s)	1229, 1684
HpyCH4V	TGCA	9 cut(s)	43, 185, 305, 725, 901, 965, 1182, 1200, 1691
HpyF10VI	GCNNNNNNNGC	8 cut(s)	26, 191, 486, 537, 602, 611, 919, 928
HpyF3I	CTNAG	9 cut(s)	86, 141, 400, 780, 939, 1317, 1386, 1635, 1701
HpySE526I	ACGT	2 cut(s)	1229, 1684
Hsp92I	GRCGYC	1 cut(s)	1772
Hsp92II	CATG	9 cut(s)	182, 493, 586, 867, 1000, 1327, 1373, 1412, 1783
HspAI	GCGC	1 cut(s)	1772
KasI	GGCGCC	1 cut(s)	1771
Ksp22I	TGATCA	2 cut(s)	355, 1798
Kzo9I	GATC	6 cut(s)	242, 355, 811, 1078, 1704, 1798
LmnI	GCTCC	4 cut(s)	142, 425, 1225, 1798
Lsp1109I	GCAGC	6 cut(s)	203, 498, 552, 931, 1549, 1802
LweI	GCATC	4 cut(s)	314, 564, 1209, 1330
MaeI	CTAG	5 cut(s)	447, 752, 1254, 1589, 1823
MaeII	ACGT	2 cut(s)	1229, 1684
MaeIII	GTNAC	5 cut(s)	115, 259, 285, 1016, 1295
MalI	GATC	6 cut(s)	244, 357, 813, 1080, 1706, 1800
MbiI	CCGCTC	1 cut(s)	705
MboI	GATC	6 cut(s)	242, 355, 811, 1078, 1704, 1798
MboII	GAAGA	5 cut(s)	379, 764, 899, 1061, 1116
MfeI	CAATTG	1 cut(s)	92
MflI	RGATCY	2 cut(s)	242, 1704
MhlI	GDGCHC	5 cut(s)	64, 533, 781, 1143, 1323
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	1772
MlyI	GAGTC	1 cut(s)	1465
MmeI	TCCRAC	4 cut(s)	675, 851, 1130, 1228
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	307
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	2 cut(s)	1023, 1395
MslI	CAYNNNNRTG	1 cut(s)	1413
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	341
MspCI	CTTAAG	1 cut(s)	1022
MspI	CCGG	2 cut(s)	57, 1345
MspR9I	CCNGG	4 cut(s)	888, 924, 1040, 1345
MunI	CAATTG	1 cut(s)	92
Mva1269I	GAATGC	1 cut(s)	307
MvaI	CCWGG	3 cut(s)	888, 924, 1040
MvnI	CGCG	1 cut(s)	1243
MwoI	GCNNNNNNNGC	8 cut(s)	26, 191, 486, 537, 602, 611, 919, 928
NarI	GGCGCC	1 cut(s)	1772
NciI	CCSGG	1 cut(s)	1345
NdeII	GATC	6 cut(s)	242, 355, 811, 1078, 1704, 1798
NlaIII	CATG	9 cut(s)	182, 493, 586, 867, 1000, 1327, 1373, 1412, 1783
NlaIV	GGNNCC	8 cut(s)	19, 138, 427, 709, 1383, 1599, 1773, 1810
NmuCI	GTSAC	2 cut(s)	285, 1016
NsiI	ATGCAT	1 cut(s)	307
NspI	RCATGY	3 cut(s)	182, 493, 1000
PaeR7I	CTCGAG	1 cut(s)	34
PagI	TCATGA	1 cut(s)	1369
PctI	GAATGC	1 cut(s)	307
PfeI	GAWTC	5 cut(s)	74, 320, 1440, 1492, 1541
PflFI	GACNNNGTC	1 cut(s)	1747
PfoI	TCCNGGA	1 cut(s)	886
PkrI	GCNGC	8 cut(s)	136, 193, 488, 542, 921, 1191, 1564, 1792
PleI	GAGTC	1 cut(s)	1465
PluTI	GGCGCC	1 cut(s)	1775
PpsI	GAGTC	1 cut(s)	1465
PpuMI	RGGWCCY	1 cut(s)	970
Psp124BI	GAGCTC	1 cut(s)	1323
Psp5II	RGGWCCY	1 cut(s)	970
Psp6I	CCWGG	3 cut(s)	886, 922, 1038
PspEI	GGTNACC	2 cut(s)	115, 1295
PspGI	CCWGG	3 cut(s)	886, 922, 1038
PspN4I	GGNNCC	8 cut(s)	19, 138, 427, 709, 1383, 1599, 1773, 1810
PspPI	GGNCC	1 cut(s)	970
PspPPI	RGGWCCY	1 cut(s)	970
PstNI	CAGNNNCTG	4 cut(s)	197, 245, 808, 1796
PsuI	RGATCY	2 cut(s)	242, 1704
PsyI	GACNNNGTC	1 cut(s)	1747
RsaI	GTAC	3 cut(s)	162, 550, 1366
RsaNI	GTAC	3 cut(s)	161, 549, 1365
RseI	CAYNNNNRTG	1 cut(s)	1413
SacI	GAGCTC	1 cut(s)	1323
SaqAI	TTAA	2 cut(s)	1023, 1395
SatI	GCNGC	8 cut(s)	135, 192, 487, 541, 920, 1190, 1563, 1791
Sau3AI	GATC	6 cut(s)	242, 355, 811, 1078, 1704, 1798
Sau96I	GGNCC	1 cut(s)	970
SchI	GAGTC	1 cut(s)	1465
ScrFI	CCNGG	4 cut(s)	888, 924, 1040, 1345
SduI	GDGCHC	5 cut(s)	64, 533, 781, 1143, 1323
SfaNI	GCATC	4 cut(s)	314, 564, 1209, 1330
SfcI	CTRYAG	1 cut(s)	800
SfoI	GGCGCC	1 cut(s)	1773
Sfr274I	CTCGAG	1 cut(s)	34
SinI	GGWCC	1 cut(s)	970
SlaI	CTCGAG	1 cut(s)	34
SmiMI	CAYNNNNRTG	1 cut(s)	1413
SmlI	CTYRAG	4 cut(s)	34, 296, 389, 1022
SmoI	CTYRAG	4 cut(s)	34, 296, 389, 1022
SpeI	ACTAGT	1 cut(s)	446
SsiI	CCGC	8 cut(s)	134, 341, 705, 1190, 1241, 1531, 1601, 1697
SspDI	GGCGCC	1 cut(s)	1771
SspMI	CTAG	5 cut(s)	447, 752, 1254, 1589, 1823
SstI	GAGCTC	1 cut(s)	1323
StyD4I	CCNGG	4 cut(s)	886, 922, 1038, 1343
TaaI	ACNGT	2 cut(s)	70, 804
TaiI	ACGT	2 cut(s)	1232, 1687
TaqI	TCGA	4 cut(s)	35, 275, 1495, 1754
TatI	WGTACW	1 cut(s)	160
TauI	GCSGC	2 cut(s)	137, 1192
TfiI	GAWTC	5 cut(s)	74, 320, 1440, 1492, 1541
Tru1I	TTAA	2 cut(s)	1023, 1395
Tru9I	TTAA	2 cut(s)	1023, 1395
TscAI	CASTG	1 cut(s)	526
TseFI	GTSAC	2 cut(s)	285, 1016
TseI	GCWGC	6 cut(s)	191, 486, 540, 919, 1562, 1790
Tsp45I	GTSAC	2 cut(s)	285, 1016
TspDTI	ATGAA	6 cut(s)	159, 270, 396, 743, 852, 1799
TspGWI	ACGGA	1 cut(s)	1079
TspRI	CASTG	1 cut(s)	526
Tth111I	GACNNNGTC	1 cut(s)	1747
Vha464I	CTTAAG	1 cut(s)	1022
VpaK11BI	GGWCC	1 cut(s)	970
XapI	RAATTY	7 cut(s)	166, 226, 271, 463, 857, 953, 1826
XceI	RCATGY	3 cut(s)	182, 493, 1000
XcmI	CCANNNNNNNNNTGG	1 cut(s)	127
XhoI	CTCGAG	1 cut(s)	34
XspI	CTAG	5 cut(s)	447, 752, 1254, 1589, 1823
Zsp2I	ATGCAT	1 cut(s)	307

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.