MD03G1032400.v1.1

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002738
Physical Location & Seq
Reverse (-)
2612660 .. 2613966
1307 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1032400.v1.1.491

Sequence Viewer

Length: 762 bp
ATGGAGGAGGTTAAAGCAAAAACTTCAGAACTTGAAAAAGAGCTGGAATTGGTAAAAGAAGCAGCAGATATGGCATTTGACGTCCATCATTCTGTTGAATTTCACTTTGAGAAACTTAGTGAGCAAGTAGATGTTCGAAGGCATCACCTTGGGGAATTGACATCACAGTGGGAAGCAGTCAGAAAGCCTTTGGAAGCAAAAAAGGAAAGTCTTCAGGTGTCTGTATATGCAAACAACCCATATGCTCAGGAAAAGCTCAGTCAGCTGAAAGATTATGAACTGGAAAAAGAATCCATCTTATTTGAAATTCGAAAAAGGGAGGAGGAACTTTCTAAACTGTCAACGGATCTAGAGAAGCAGCCCAAGCTGGCATCAAGAAAGTCCTACATTGAAAGGGGAAAGGAGATCACAAAGAACAGTCGCAAACAAGAAGCTGACATAAAGCAGATCTTAAAAGACACCAGGGAGCTTCATTTAGAGAGTAACTCTATCCAAGAACGTCTTCATCGGACGTATGCTGTTGTAGATGAAATGGTGTTCAGGGAAGCAAAGAAAGATGCAGTTGGGAAGCAGGCTTATAGGCTTCTCACTAGCATCCACGAGAGCTTCGAACAGATTCGTGAGAAAATCTTGACAACTGATAGAATCCGACGAGAAGTAGGTGAACATGAAAAGAAGCTAGCAGCCATGGCCTCGCGAAGCTTAAACGTAGACAAACTACAAGCCGATCTTGATGCCATCAGGAGGGAAAATGATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF812

Protein Analysis

254

Amino Acids

29.78

Weight (kDa)

6.2

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC22_CC PF05667 2 - 228 3.2e-47 CCDC22 protein coiled-coil region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 84
AccI GTMKAC 1 cut(s) 711
AccII CGCG 1 cut(s) 697
AclWI GGATC 1 cut(s) 354
AcsI RAATTY 2 cut(s) 98, 306
AcuI CTGAAG 2 cut(s) 9, 197
AcyI GRCGYC 1 cut(s) 81
AfiI CCNNNNNNNGG 1 cut(s) 744
AgsI TTSAA 4 cut(s) 35, 98, 305, 392
AjnI CCWGG 1 cut(s) 461
AjuI GAANNNNNNNTTGG 2 cut(s) 486, 518
AluBI AGCT 9 cut(s) 43, 256, 265, 367, 434, 469, 606, 679, 702
AluI AGCT 9 cut(s) 43, 256, 265, 367, 434, 469, 606, 679, 702
AlwI GGATC 1 cut(s) 354
AoxI GGCC 1 cut(s) 690
ApeKI GCWGC 3 cut(s) 62, 358, 683
ApoI RAATTY 2 cut(s) 98, 306
Asp700I GAANNNNTTC 3 cut(s) 210, 501, 615
AsuHPI GGTGA 2 cut(s) 137, 674
AsuII TTCGAA 3 cut(s) 136, 310, 609
AsuNHI GCTAGC 1 cut(s) 679
BauI CACGAG 1 cut(s) 599
BbsI GAAGAC 2 cut(s) 203, 494
BbvI GCAGC 3 cut(s) 74, 370, 695
BccI CCATC 3 cut(s) 93, 302, 746
BcgI CGANNNNNNTGC 2 cut(s) 716, 750
BciT130I CCWGG 1 cut(s) 463
BfaI CTAG 3 cut(s) 350, 591, 680
BglII AGATCT 1 cut(s) 447
BisI GCNGC 3 cut(s) 63, 359, 684
BlsI GCNGC 3 cut(s) 64, 360, 685
Bme1390I CCNGG 1 cut(s) 463
BmrFI CCNGG 1 cut(s) 463
BmsI GCATC 5 cut(s) 151, 380, 547, 603, 724
BmtI GCTAGC 1 cut(s) 683
BpiI GAAGAC 2 cut(s) 203, 494
BplI GAGNNNNNCTC 2 cut(s) 470, 502
Bpu10I CCTNAGC 1 cut(s) 246
Bpu14I TTCGAA 3 cut(s) 136, 310, 609
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 3 cut(s) 148, 462, 687
Bsc4I CCNNNNNNNGG 1 cut(s) 744
Bse1I ACTGG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 463
BseDI CCNNGG 3 cut(s) 148, 462, 687
BseGI GGATG 1 cut(s) 594
BseLI CCNNNNNNNGG 1 cut(s) 744
BseMII CTCAG 2 cut(s) 260, 271
BseNI ACTGG 1 cut(s) 285
BseRI GAGGAG 2 cut(s) 20, 335
BseXI GCAGC 3 cut(s) 74, 370, 695
Bsh1236I CGCG 1 cut(s) 697
BshFI GGCC 1 cut(s) 692
BslI CCNNNNNNNGG 1 cut(s) 744
BsnI GGCC 1 cut(s) 692
Bsp119I TTCGAA 3 cut(s) 136, 310, 609
Bsp143I GATC 4 cut(s) 346, 405, 447, 727
Bsp19I CCATGG 1 cut(s) 687
Bsp68I TCGCGA 1 cut(s) 697
BspANI GGCC 1 cut(s) 692
BspCNI CTCAG 2 cut(s) 259, 270
BspFNI CGCG 1 cut(s) 697
BspOI GCTAGC 1 cut(s) 683
BspPI GGATC 1 cut(s) 354
BspT104I TTCGAA 3 cut(s) 136, 310, 609
BsrI ACTGG 1 cut(s) 285
BssECI CCNNGG 3 cut(s) 148, 462, 687
BssMI GATC 4 cut(s) 346, 405, 447, 727
BssNI GRCGYC 1 cut(s) 81
BssSI CACGAG 1 cut(s) 599
BssT1I CCWWGG 2 cut(s) 148, 687
Bst2BI CACGAG 1 cut(s) 599
Bst2UI CCWGG 1 cut(s) 463
Bst4CI ACNGT 3 cut(s) 168, 339, 419
BstACI GRCGYC 1 cut(s) 81
BstBI TTCGAA 3 cut(s) 136, 310, 609
BstC8I GCNNGC 3 cut(s) 369, 573, 681
BstDEI CTNAG 3 cut(s) 116, 246, 257
BstDSI CCRYGG 1 cut(s) 687
BstF5I GGATG 1 cut(s) 594
BstFNI CGCG 1 cut(s) 697
BstKTI GATC 4 cut(s) 349, 408, 450, 730
BstMBI GATC 4 cut(s) 346, 405, 447, 727
BstMWI GCNNNNNNNGC 4 cut(s) 71, 262, 364, 689
BstNI CCWGG 1 cut(s) 463
BstSCI CCNGG 1 cut(s) 461
BstUI CGCG 1 cut(s) 697
BstV1I GCAGC 3 cut(s) 74, 370, 695
BstV2I GAAGAC 2 cut(s) 203, 494
BstX2I RGATCY 2 cut(s) 346, 447
BstYI RGATCY 2 cut(s) 346, 447
BsuRI GGCC 1 cut(s) 692
BtgI CCRYGG 1 cut(s) 687
BtsCI GGATG 1 cut(s) 594
BtsIMutI CAGTG 1 cut(s) 173
BtuMI TCGCGA 1 cut(s) 697
Cac8I GCNNGC 3 cut(s) 369, 573, 681
CviAII CATG 2 cut(s) 668, 688
DdeI CTNAG 3 cut(s) 116, 246, 257
DpnI GATC 4 cut(s) 348, 407, 449, 729
DpnII GATC 4 cut(s) 346, 405, 447, 727
Eco130I CCWWGG 2 cut(s) 148, 687
Eco57I CTGAAG 2 cut(s) 9, 197
EcoRII CCWGG 1 cut(s) 461
EcoT14I CCWWGG 2 cut(s) 148, 687
ErhI CCWWGG 2 cut(s) 148, 687
FaeI CATG 2 cut(s) 671, 691
FalI AAGNNNNNCTT 6 cut(s) 434, 466, 486, 518, 714, 746
FatI CATG 2 cut(s) 667, 687
FauNDI CATATG 1 cut(s) 241
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 3 cut(s) 63, 359, 684
FokI GGATG 1 cut(s) 581
Fsp4HI GCNGC 3 cut(s) 63, 359, 684
FspBI CTAG 3 cut(s) 350, 591, 680
GluI GCNGC 3 cut(s) 63, 359, 684
HaeIII GGCC 1 cut(s) 692
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 2 cut(s) 671, 691
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 700
HinfI GANTC 3 cut(s) 290, 616, 645
HphI GGTGA 2 cut(s) 137, 674
Hpy166II GTNNAC 3 cut(s) 342, 665, 712
Hpy188I TCNGA 4 cut(s) 28, 182, 510, 650
Hpy188III TCNNGA 8 cut(s) 248, 350, 375, 620, 631, 696, 731, 742
Hpy8I GTNNAC 3 cut(s) 342, 665, 712
Hpy99I CGWCG 1 cut(s) 654
HpyAV CCTTC 1 cut(s) 132
HpyCH4III ACNGT 3 cut(s) 168, 339, 419
HpyCH4IV ACGT 4 cut(s) 81, 499, 512, 708
HpyCH4V TGCA 2 cut(s) 230, 560
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 262, 364, 689
HpyF3I CTNAG 3 cut(s) 116, 246, 257
HpySE526I ACGT 4 cut(s) 81, 499, 512, 708
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 2 cut(s) 671, 691
Kzo9I GATC 4 cut(s) 346, 405, 447, 727
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 3 cut(s) 74, 370, 695
LweI GCATC 5 cut(s) 151, 380, 547, 603, 724
MaeI CTAG 3 cut(s) 350, 591, 680
MaeII ACGT 4 cut(s) 81, 499, 512, 708
MaeIII GTNAC 1 cut(s) 482
MalI GATC 4 cut(s) 348, 407, 449, 729
MboI GATC 4 cut(s) 346, 405, 447, 727
MboII GAAGA 2 cut(s) 203, 494
MflI RGATCY 2 cut(s) 346, 447
MluCI AATT 4 cut(s) 47, 98, 155, 306
MmeI TCCRAC 1 cut(s) 673
MnlI CCTC 4 cut(s) 313, 316, 703, 738
MroXI GAANNNNTTC 3 cut(s) 210, 501, 615
MseI TTAA 4 cut(s) 12, 452, 704, 760
MslI CAYNNNNRTG 1 cut(s) 166
MspA1I CMGCKG 1 cut(s) 265
MspR9I CCNGG 1 cut(s) 463
MvaI CCWGG 1 cut(s) 463
MvnI CGCG 1 cut(s) 697
MwoI GCNNNNNNNGC 4 cut(s) 71, 262, 364, 689
NcoI CCATGG 1 cut(s) 687
NdeI CATATG 1 cut(s) 241
NdeII GATC 4 cut(s) 346, 405, 447, 727
NheI GCTAGC 1 cut(s) 679
NlaIII CATG 2 cut(s) 671, 691
NruI TCGCGA 1 cut(s) 697
NspV TTCGAA 3 cut(s) 136, 310, 609
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PdmI GAANNNNTTC 3 cut(s) 210, 501, 615
PfeI GAWTC 3 cut(s) 290, 616, 645
PkrI GCNGC 3 cut(s) 64, 360, 685
Psp6I CCWGG 1 cut(s) 461
PspGI CCWGG 1 cut(s) 461
PsuI RGATCY 2 cut(s) 346, 447
PvuII CAGCTG 1 cut(s) 265
RruI TCGCGA 1 cut(s) 697
RseI CAYNNNNRTG 1 cut(s) 166
SaqAI TTAA 4 cut(s) 12, 452, 704, 760
SatI GCNGC 3 cut(s) 63, 359, 684
Sau3AI GATC 4 cut(s) 346, 405, 447, 727
ScrFI CCNGG 1 cut(s) 463
SfaNI GCATC 5 cut(s) 151, 380, 547, 603, 724
SfuI TTCGAA 3 cut(s) 136, 310, 609
SmiMI CAYNNNNRTG 1 cut(s) 166
Sse9I AATT 4 cut(s) 47, 98, 155, 306
SspMI CTAG 3 cut(s) 350, 591, 680
StyD4I CCNGG 1 cut(s) 461
StyI CCWWGG 2 cut(s) 148, 687
TaaI ACNGT 3 cut(s) 168, 339, 419
TaiI ACGT 4 cut(s) 84, 502, 515, 711
TaqI TCGA 3 cut(s) 136, 310, 609
TasI AATT 4 cut(s) 47, 98, 155, 306
TfiI GAWTC 3 cut(s) 290, 616, 645
Tru1I TTAA 4 cut(s) 12, 452, 704, 760
Tru9I TTAA 4 cut(s) 12, 452, 704, 760
TscAI CASTG 1 cut(s) 173
TseI GCWGC 3 cut(s) 62, 358, 683
TspDTI ATGAA 5 cut(s) 291, 461, 494, 543, 684
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 1 cut(s) 173
XapI RAATTY 2 cut(s) 98, 306
XbaI TCTAGA 1 cut(s) 349
XmiI GTMKAC 1 cut(s) 711
XmnI GAANNNNTTC 3 cut(s) 210, 501, 615
XspI CTAG 3 cut(s) 350, 591, 680
ZraI GACGTC 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.