Rorug05G0443800

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002738
Physical Location & Seq
Forward (+)
61507141 .. 61508422
1282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0443800.1

Sequence Viewer

Length: 1182 bp
ATGGGTTTGAGGAGAATCGGTCCTGATGTTTATACTCTAACTATCATCATTAACTGCTATTGTCATTTGAACCATATGGGGTTCAGTTTGTCTGTCTTGGGTCACTTCTTCAAGTCTGGATTTGAACCAAATGTCACCACTTTGATCAATGGCGATAGAGTTGCCGAGGCTACACAAATTTTCAGAAAAATGATGGAGGGAGGTACTTGTAAGCCCGATGTGGTTACTTTCGGCACATTGATAAAGGGCTTGTGCATCAAGGGCAACAATGGTGGAGCTATTCAACTGCTCAGGAGGATGGAACTATCTCCAGATTGTAAGCCTAATTTAGTTGCTTGTAACACCGTCATTGACAGCCTTTGTAAGGATACACTAATTGCTGATGCCTTCAATCTCTTCTCGGAAATGACTATTGCCCCTGACGTCATTACATATACGTCCTTGATTCATGGAGTTTGCAGCTTAGGCCAGTGGAAACAAGCTATGAGGTTGTTAAATGAAATGGTCAATGAAAAGATATTTCCAAATGTGCACACCTTCACCGTCTTGGTAGATACACTTTGTAAGGAGGGGATGGTCTTGGAAGCGAAAAGCGTGATTGAAATGATGATTCAAAGAGATATTGAACCTGATACAGTTACATACACTGCACTTATGGATGGATACTGTTTGAGAGGTGAAATGGGGGAGGCAAGACAAGTCTTCGATCTAATGGAAATGTCTCGTAAGGGATTGTTTCCAGATCTTGTGACTTGTGCTGTTTTACTGGATGGCCTTTGTAAAAACCGACAACTTGGTATGGCAATAGAGTTGCTTAGAGAGATGGAAGGCAAGAAATTGCATCCAGATATTGTAATTTACAATATTCTCATGGATTATTTTATCAAAGCTGGAAAAGTTGAAGCTGCAAGGGATCTATTTTGCTCTTTATCATCAAAAGGTCTTCATCCTAGTGTTAAGACATATACTACAATGATTAGTGGATTTTGTTACGATGGCCGAATTAGTGAAGCAGAAACATTGCTTAGAGAAATGGAAGAGAGAGGCTGTTCTCCAAATGATGTTACCTACAACACATTTATCCGAGGGCTTATCAATAACAATGAGACATCAAGGGCGATGGGTCTTATTCATCAAATGGTGCAGGGGGTTTCTCTGCAGATGCATCAACAATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF812

Protein Analysis

393

Amino Acids

44.02

Weight (kDa)

6.02

Isoelectric Point (pI)

27.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 52 - 85 7.4e-06 PPR repeat family
PPR_1 PF12854 70 - 101 1.7e-10 PPR repeat
PPR_1 PF12854 106 - 137 1.9e-07 PPR repeat
PPR_2 PF13041 108 - 136 7.2e-06 PPR repeat family
PPR_long PF17177 121 - 243 5.5e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 138 - 187 1.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 139 - 169 1.5e-10 PPR repeat
PPR_2 PF13041 140 - 189 1.5e-17 PPR repeat family
PPR PF01535 143 - 172 2.3e-06 PPR repeat
PPR_1 PF12854 173 - 203 6e-07 PPR repeat
PPR_2 PF13041 183 - 223 1.1e-09 PPR repeat family
TPR_24 PF23276 196 - 307 1.7e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 207 - 239 1.7e-10 PPR repeat
PPR_2 PF13041 210 - 261 1.6e-13 PPR repeat family
PPR PF01535 213 - 240 1.4e-06 PPR repeat
PPR_3 PF13812 238 - 294 4.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 243 - 276 1.3e-06 PPR repeat
PPR_2 PF13041 247 - 284 2.8e-07 PPR repeat family
TPR_24 PF23276 267 - 376 8.3e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 282 - 330 1.2e-16 PPR repeat family
PPR_3 PF13812 310 - 362 2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 313 - 346 5.2e-12 PPR repeat
PPR_2 PF13041 317 - 365 3.8e-17 PPR repeat family
PPR PF01535 321 - 350 4.4e-09 PPR repeat
PPR_1 PF12854 348 - 381 1.2e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 426
AclWI GGATC 1 cut(s) 921
AcoI YGGCCR 1 cut(s) 997
AcsI RAATTY 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 423
AfaI GTAC 1 cut(s) 205
AfiI CCNNNNNNNGG 1 cut(s) 364
AgsI TTSAA 9 cut(s) 70, 112, 125, 284, 391, 602, 614, 626, 902
AluBI AGCT 5 cut(s) 278, 462, 482, 890, 905
AluI AGCT 5 cut(s) 278, 462, 482, 890, 905
Alw21I GWGCWC 1 cut(s) 534
Alw26I GTCTC 2 cut(s) 726, 1100
Alw44I GTGCAC 1 cut(s) 530
AlwI GGATC 1 cut(s) 921
AoxI GGCC 3 cut(s) 466, 772, 997
ApaLI GTGCAC 1 cut(s) 530
ApeKI GCWGC 2 cut(s) 459, 905
ApoI RAATTY 1 cut(s) 177
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 3 cut(s) 127, 532, 689
AvaII GGWCC 1 cut(s) 20
BaeGI GKGCMC 1 cut(s) 534
BbsI GAAGAC 2 cut(s) 694, 935
Bbv12I GWGCWC 1 cut(s) 534
BbvI GCAGC 2 cut(s) 471, 892
BccI CCATC 8 cut(s) 187, 292, 568, 653, 764, 817, 989, 1114
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciVI GTATCC 2 cut(s) 361, 656
BclI TGATCA 1 cut(s) 144
BcoDI GTCTC 2 cut(s) 726, 1100
BfaI CTAG 1 cut(s) 951
BfmI CTRYAG 1 cut(s) 1157
BfuI GTATCC 2 cut(s) 361, 656
BglII AGATCT 1 cut(s) 742
BisI GCNGC 2 cut(s) 460, 906
BlsI GCNGC 2 cut(s) 461, 907
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 20
BmsI GCATC 5 cut(s) 264, 373, 850, 1152, 1174
BpiI GAAGAC 2 cut(s) 694, 935
BpmI CTGGAG 1 cut(s) 294
Bpu10I CCTNAGC 2 cut(s) 290, 463
BsaHI GRCGYC 1 cut(s) 423
BsaJI CCNNGG 2 cut(s) 165, 1084
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 1 cut(s) 364
Bse1I ACTGG 2 cut(s) 469, 771
Bse3DI GCAATG 1 cut(s) 1019
BseDI CCNNGG 2 cut(s) 165, 1084
BseGI GGATG 6 cut(s) 303, 579, 664, 775, 841, 946
BseLI CCNNNNNNNGG 1 cut(s) 364
BseMI GCAATG 1 cut(s) 1019
BseMII CTCAG 1 cut(s) 304
BseNI ACTGG 2 cut(s) 469, 771
BseRI GAGGAG 1 cut(s) 25
BseSI GKGCMC 1 cut(s) 534
BseXI GCAGC 2 cut(s) 471, 892
BsgI GTGCAG 2 cut(s) 633, 1163
BshFI GGCC 3 cut(s) 468, 774, 999
BsiHKAI GWGCWC 1 cut(s) 534
BslI CCNNNNNNNGG 1 cut(s) 364
BsmAI GTCTC 2 cut(s) 726, 1100
BsnI GGCC 3 cut(s) 468, 774, 999
Bsp1286I GDGCHC 1 cut(s) 534
Bsp143I GATC 4 cut(s) 144, 706, 742, 913
BspANI GGCC 3 cut(s) 468, 774, 999
BspCNI CTCAG 1 cut(s) 303
BspMAI CTGCAG 1 cut(s) 1161
BspPI GGATC 1 cut(s) 921
BsrDI GCAATG 1 cut(s) 1019
BsrI ACTGG 2 cut(s) 469, 771
BssECI CCNNGG 2 cut(s) 165, 1084
BssMI GATC 4 cut(s) 144, 706, 742, 913
BssNI GRCGYC 1 cut(s) 423
Bst4CI ACNGT 4 cut(s) 346, 544, 637, 668
Bst6I CTCTTC 2 cut(s) 401, 1032
BstACI GRCGYC 1 cut(s) 423
BstDEI CTNAG 4 cut(s) 290, 463, 815, 1025
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 6 cut(s) 303, 579, 664, 775, 841, 946
BstKTI GATC 4 cut(s) 147, 709, 745, 916
BstMAI GTCTC 2 cut(s) 726, 1100
BstMBI GATC 4 cut(s) 144, 706, 742, 913
BstMWI GCNNNNNNNGC 2 cut(s) 261, 465
BstSFI CTRYAG 1 cut(s) 1157
BstSLI GKGCMC 1 cut(s) 534
BstV1I GCAGC 2 cut(s) 471, 892
BstV2I GAAGAC 2 cut(s) 694, 935
BstX2I RGATCY 2 cut(s) 742, 913
BstYI RGATCY 2 cut(s) 742, 913
BsuI GTATCC 2 cut(s) 361, 656
BsuRI GGCC 3 cut(s) 468, 774, 999
BtgZI GCGATG 1 cut(s) 1133
BtsCI GGATG 6 cut(s) 303, 579, 664, 775, 841, 946
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 2 cut(s) 476, 645
Cfr13I GGNCC 1 cut(s) 20
Csp6I GTAC 1 cut(s) 204
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 2 cut(s) 449, 871
CviQI GTAC 1 cut(s) 204
DdeI CTNAG 4 cut(s) 290, 463, 815, 1025
DpnI GATC 4 cut(s) 146, 708, 744, 915
DpnII GATC 4 cut(s) 144, 706, 742, 913
EaeI YGGCCR 1 cut(s) 997
Eam1104I CTCTTC 2 cut(s) 401, 1032
EarI CTCTTC 2 cut(s) 401, 1032
Eco47I GGWCC 1 cut(s) 20
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoT22I ATGCAT 1 cut(s) 1167
FaeI CATG 2 cut(s) 452, 874
FatI CATG 2 cut(s) 448, 870
FauNDI CATATG 1 cut(s) 75
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 2 cut(s) 460, 906
FokI GGATG 6 cut(s) 310, 586, 671, 782, 828, 933
Fsp4HI GCNGC 2 cut(s) 460, 906
FspBI CTAG 1 cut(s) 951
GluI GCNGC 2 cut(s) 460, 906
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 3 cut(s) 468, 774, 999
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 2 cut(s) 452, 874
HinfI GANTC 3 cut(s) 15, 445, 610
HphI GGTGA 3 cut(s) 127, 532, 689
Hpy166II GTNNAC 1 cut(s) 532
Hpy188I TCNGA 3 cut(s) 185, 403, 1085
Hpy188III TCNNGA 6 cut(s) 23, 117, 292, 311, 740, 845
Hpy8I GTNNAC 1 cut(s) 532
HpyAV CCTTC 3 cut(s) 397, 547, 821
HpyCH4III ACNGT 4 cut(s) 346, 544, 637, 668
HpyCH4IV ACGT 2 cut(s) 423, 437
HpyCH4V TGCA 9 cut(s) 255, 459, 532, 650, 841, 908, 1144, 1159, 1165
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 465
HpyF3I CTNAG 4 cut(s) 290, 463, 815, 1025
HpySE526I ACGT 2 cut(s) 423, 437
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 2 cut(s) 452, 874
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 4 cut(s) 144, 706, 742, 913
LmnI GCTCC 1 cut(s) 275
Lsp1109I GCAGC 2 cut(s) 471, 892
LweI GCATC 5 cut(s) 264, 373, 850, 1152, 1174
MaeI CTAG 1 cut(s) 951
MaeII ACGT 2 cut(s) 423, 437
MaeIII GTNAC 8 cut(s) 101, 133, 223, 338, 637, 748, 989, 1063
MalI GATC 4 cut(s) 146, 708, 744, 915
MboI GATC 4 cut(s) 144, 706, 742, 913
MboII GAAGA 5 cut(s) 100, 388, 694, 935, 1049
MflI RGATCY 2 cut(s) 742, 913
MhlI GDGCHC 1 cut(s) 534
MluCI AATT 6 cut(s) 177, 325, 375, 836, 855, 1002
Mph1103I ATGCAT 1 cut(s) 1167
MseI TTAA 3 cut(s) 51, 494, 957
MslI CAYNNNNRTG 1 cut(s) 951
MwoI GCNNNNNNNGC 2 cut(s) 261, 465
NdeI CATATG 1 cut(s) 75
NdeII GATC 4 cut(s) 144, 706, 742, 913
NlaIII CATG 2 cut(s) 452, 874
NmeAIII GCCGAG 1 cut(s) 190
NmuCI GTSAC 3 cut(s) 101, 133, 748
NsiI ATGCAT 1 cut(s) 1167
PfeI GAWTC 3 cut(s) 15, 445, 610
PkrI GCNGC 2 cut(s) 461, 907
PspPI GGNCC 1 cut(s) 20
PstI CTGCAG 1 cut(s) 1161
PsuI RGATCY 2 cut(s) 742, 913
RsaI GTAC 1 cut(s) 205
RsaNI GTAC 1 cut(s) 204
RseI CAYNNNNRTG 1 cut(s) 951
SaqAI TTAA 3 cut(s) 51, 494, 957
SatI GCNGC 2 cut(s) 460, 906
Sau3AI GATC 4 cut(s) 144, 706, 742, 913
Sau96I GGNCC 1 cut(s) 20
SduI GDGCHC 1 cut(s) 534
SfaNI GCATC 5 cut(s) 264, 373, 850, 1152, 1174
SfcI CTRYAG 1 cut(s) 1157
SinI GGWCC 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 951
Sse9I AATT 6 cut(s) 177, 325, 375, 836, 855, 1002
SspI AATATT 1 cut(s) 865
SspMI CTAG 1 cut(s) 951
TaaI ACNGT 4 cut(s) 346, 544, 637, 668
TaiI ACGT 2 cut(s) 426, 440
TaqI TCGA 1 cut(s) 705
TaqII GACCGA 1 cut(s) 8
TasI AATT 6 cut(s) 177, 325, 375, 836, 855, 1002
TfiI GAWTC 3 cut(s) 15, 445, 610
Tru1I TTAA 3 cut(s) 51, 494, 957
Tru9I TTAA 3 cut(s) 51, 494, 957
TscAI CASTG 2 cut(s) 476, 652
TseFI GTSAC 3 cut(s) 101, 133, 748
TseI GCWGC 2 cut(s) 459, 905
Tsp45I GTSAC 3 cut(s) 101, 133, 748
TspDTI ATGAA 5 cut(s) 437, 513, 525, 935, 1121
TspRI CASTG 2 cut(s) 476, 652
VneI GTGCAC 1 cut(s) 530
VpaK11BI GGWCC 1 cut(s) 20
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 1 cut(s) 177
XspI CTAG 1 cut(s) 951
ZraI GACGTC 1 cut(s) 424
Zsp2I ATGCAT 1 cut(s) 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.