Rh5CG545600

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002738
Physical Location & Seq
Forward (+)
76101975 .. 76104758
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG545600.1

Sequence Viewer

Length: 1191 bp
ATGCTTAGAAACAGACTATATGTGTTGCTTAGGCAGTTCTTGTATCCATCTGAAGAGGACTCGTGTAAGTTGGTAAGGTTCTTGGTGGAGAGGCTTTCGGAGTCGTCCGGGAGTGGAAGCAGGGGCGGTGTCGAGGGTGTTGAGGATGTGGAGAGAGTGAAAGAAGATAGCTTTGAAAGCAATGTGGGGAACCACAGAAGCGGTGATGACAGACTTGGACCGAAATGGGATGAATTCACATTGAATGGTGAAGAGTCTGAAGTATTGAACATCGAGGGTGAAGATGCCTCTGTTGGTTGTGATGCTAATCATATGCTGCGAAGATTGGATGAAATGAGTATTGACAATGTATCCAGCAGACCTGATGATCTTCATTCGTCAGAGGAGAAACAGCTTGAGGAGGAGGTGATGGCAAAGACTTCAGAATTGAAGCACCTTGAAGAAGAGTTGGAATTGTTAAAAGAAGCAGTAGATATAGCATTTGATGTCGATCATCCCGCTGAATTTTACCTTGAGAAACTTAATCAGCAAGTGGATGCTCAAAGGCAACACCTTGGAGAATTGAAGTCACAGCAGGAAGTTGTCAGAAGGCCTTTGGAAGAGAAGAGGAGAACTCTTCTGGAGACTCTGTCTGCAGATAACCCAGATGCTAAGAAAAAGCTCCAAAAGCTGAAACAGCTTGAAGTGGAAAAGGAATCCATGTTAGCTTCAATTCGAAAAAGGGAGGAGGAACTGTCTCAACTTACTATGGATCTAGAGAAGCAGCCCAAACTGGCATCAAGGAGATCTTATATTGAAAGGGTCAAAGAGATCACAAAAAATAGTCGGAAGCAAGAAGCTGACATAGAGCAGATTTTAAAGGACACCAGGGAGCTTCAGTTAGAGAGTAATTCTATTCAAGAACGCCTCCATCGGACATATGCTGTTGTAGATGAGCTTGTTTTCAGGGAAGCAAAGAAAGATGCAGTAGGGAGGCAGGCTTATAGGCTCCTCACTAGCATCCACGAGAGCTTTGAACAAATTCGTGAGAAAATCCTGGCAACTGATAGATTACGTCGAGAAGCGACTGAACATGAAAAGAAGCTAGCAGCCATGGCTTCACGGAGCTTAAATGCAGACAAACTACAAGCAGATCTTGAGGTCATTAGGAGAGAGAATGAGTACCTAGAGAAATCTCTCCAGGATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF812

Protein Analysis

396

Amino Acids

46.04

Weight (kDa)

5.14

Isoelectric Point (pI)

62.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC22_CC PF05667 127 - 364 5.8e-50 CCDC22 protein coiled-coil region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 126, 201, 498
AclWI GGATC 1 cut(s) 759
AcsI RAATTY 3 cut(s) 233, 503, 1020
AcuI CTGAAG 4 cut(s) 72, 279, 405, 860
AfaI GTAC 1 cut(s) 1163
AjnI CCWGG 3 cut(s) 866, 1035, 1179
Alw26I GTCTC 2 cut(s) 617, 741
AlwI GGATC 1 cut(s) 759
AoxI GGCC 1 cut(s) 590
ApeKI GCWGC 3 cut(s) 316, 763, 1088
ApoI RAATTY 3 cut(s) 233, 503, 1020
Asp700I GAANNNNTTC 1 cut(s) 1020
AspS9I GGNCC 1 cut(s) 218
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 4 cut(s) 215, 260, 290, 418
AsuII TTCGAA 1 cut(s) 715
AsuNHI GCTAGC 1 cut(s) 1084
AvaII GGWCC 1 cut(s) 218
BauI CACGAG 2 cut(s) 61, 1004
BbvI GCAGC 3 cut(s) 303, 775, 1100
BccI CCATC 3 cut(s) 55, 403, 918
BciT130I CCWGG 3 cut(s) 868, 1037, 1181
BciVI GTATCC 2 cut(s) 54, 361
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 617, 741
BfaI CTAG 4 cut(s) 755, 996, 1085, 1166
BfmI CTRYAG 1 cut(s) 633
BfuI GTATCC 2 cut(s) 54, 361
BglII AGATCT 2 cut(s) 785, 1132
BisI GCNGC 3 cut(s) 317, 764, 1089
BlsI GCNGC 3 cut(s) 318, 765, 1090
Bme1390I CCNGG 4 cut(s) 109, 868, 1037, 1181
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 2 cut(s) 191, 989
BmrFI CCNGG 4 cut(s) 109, 868, 1037, 1181
BmsI GCATC 7 cut(s) 274, 292, 526, 637, 785, 952, 1008
BmtI GCTAGC 1 cut(s) 1088
BplI GAGNNNNNCTC 2 cut(s) 598, 630
BpmI CTGGAG 2 cut(s) 641, 1163
Bpu10I CCTNAGC 1 cut(s) 29
Bpu14I TTCGAA 1 cut(s) 715
BpuEI CTTGAG 3 cut(s) 416, 533, 1157
BpuMI CCSGG 1 cut(s) 109
BsaBI GATNNNNATC 2 cut(s) 306, 489
BsaJI CCNNGG 3 cut(s) 553, 867, 1092
BsaXI ACNNNNNCTCC 6 cut(s) 614, 644, 719, 749, 863, 893
Bse1I ACTGG 1 cut(s) 777
Bse3DI GCAATG 1 cut(s) 187
Bse8I GATNNNNATC 2 cut(s) 306, 489
BseBI CCWGG 3 cut(s) 868, 1037, 1181
BseDI CCNNGG 3 cut(s) 553, 867, 1092
BseGI GGATG 6 cut(s) 151, 235, 334, 493, 541, 999
BseJI GATNNNNATC 2 cut(s) 306, 489
BseMI GCAATG 1 cut(s) 187
BseNI ACTGG 1 cut(s) 777
BseRI GAGGAG 6 cut(s) 398, 413, 416, 622, 740, 980
BseXI GCAGC 3 cut(s) 303, 775, 1100
BshFI GGCC 1 cut(s) 592
BsiSI CCGG 1 cut(s) 108
BsmAI GTCTC 2 cut(s) 617, 741
BsnI GGCC 1 cut(s) 592
Bsp119I TTCGAA 1 cut(s) 715
Bsp143I GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
Bsp19I CCATGG 1 cut(s) 1092
BspACI CCGC 3 cut(s) 126, 201, 498
BspANI GGCC 1 cut(s) 592
BspLI GGNNCC 2 cut(s) 191, 989
BspMAI CTGCAG 1 cut(s) 637
BspOI GCTAGC 1 cut(s) 1088
BspPI GGATC 1 cut(s) 759
BspT104I TTCGAA 1 cut(s) 715
BsrDI GCAATG 1 cut(s) 187
BsrI ACTGG 1 cut(s) 777
BssECI CCNNGG 3 cut(s) 553, 867, 1092
BssMI GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
BssSI CACGAG 2 cut(s) 61, 1004
BssT1I CCWWGG 2 cut(s) 553, 1092
Bst2BI CACGAG 2 cut(s) 61, 1004
Bst2UI CCWGG 3 cut(s) 868, 1037, 1181
Bst4CI ACNGT 1 cut(s) 735
Bst6I CTCTTC 6 cut(s) 48, 246, 438, 594, 599, 621
BstBI TTCGAA 1 cut(s) 715
BstC8I GCNNGC 2 cut(s) 978, 1086
BstDEI CTNAG 3 cut(s) 5, 29, 651
BstDSI CCRYGG 1 cut(s) 1092
BstF5I GGATG 6 cut(s) 151, 235, 334, 493, 541, 999
BstKTI GATC 6 cut(s) 370, 493, 754, 788, 813, 1135
BstMAI GTCTC 2 cut(s) 617, 741
BstMBI GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
BstMWI GCNNNNNNNGC 4 cut(s) 177, 667, 676, 1094
BstNI CCWGG 3 cut(s) 868, 1037, 1181
BstSCI CCNGG 4 cut(s) 107, 866, 1035, 1179
BstSFI CTRYAG 1 cut(s) 633
BstV1I GCAGC 3 cut(s) 303, 775, 1100
BstX2I RGATCY 3 cut(s) 751, 785, 1132
BstYI RGATCY 3 cut(s) 751, 785, 1132
BsuI GTATCC 2 cut(s) 54, 361
BsuRI GGCC 1 cut(s) 592
BtgI CCRYGG 1 cut(s) 1092
BtsCI GGATG 6 cut(s) 151, 235, 334, 493, 541, 999
Cac8I GCNNGC 2 cut(s) 978, 1086
Cfr13I GGNCC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 1162
CviAII CATG 3 cut(s) 700, 1073, 1093
CviQI GTAC 1 cut(s) 1162
DdeI CTNAG 3 cut(s) 5, 29, 651
DpnI GATC 6 cut(s) 369, 492, 753, 787, 812, 1134
DpnII GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
DraI TTTAAA 1 cut(s) 858
Eam1104I CTCTTC 6 cut(s) 48, 246, 438, 594, 599, 621
EarI CTCTTC 6 cut(s) 48, 246, 438, 594, 599, 621
Eco130I CCWWGG 2 cut(s) 553, 1092
Eco147I AGGCCT 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 218
Eco57I CTGAAG 4 cut(s) 72, 279, 405, 860
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 3 cut(s) 866, 1035, 1179
EcoT14I CCWWGG 2 cut(s) 553, 1092
ErhI CCWWGG 2 cut(s) 553, 1092
FaeI CATG 3 cut(s) 703, 1076, 1096
FalI AAGNNNNNCTT 4 cut(s) 772, 804, 1119, 1151
FatI CATG 3 cut(s) 699, 1072, 1092
FauI CCCGC 1 cut(s) 505
FauNDI CATATG 2 cut(s) 312, 919
Fnu4HI GCNGC 3 cut(s) 317, 764, 1089
FokI GGATG 6 cut(s) 158, 242, 341, 480, 548, 986
Fsp4HI GCNGC 3 cut(s) 317, 764, 1089
FspBI CTAG 4 cut(s) 755, 996, 1085, 1166
GluI GCNGC 3 cut(s) 317, 764, 1089
GsuI CTGGAG 2 cut(s) 641, 1163
HaeIII GGCC 1 cut(s) 592
HapII CCGG 1 cut(s) 108
Hin1II CATG 3 cut(s) 703, 1076, 1096
HinfI GANTC 5 cut(s) 59, 101, 254, 625, 695
HpaII CCGG 1 cut(s) 108
HphI GGTGA 4 cut(s) 215, 260, 290, 418
Hpy188I TCNGA 8 cut(s) 52, 100, 259, 382, 424, 587, 828, 915
Hpy188III TCNNGA 6 cut(s) 620, 755, 899, 1025, 1058, 1136
Hpy99I CGWCG 1 cut(s) 1059
HpyAV CCTTC 1 cut(s) 582
HpyCH4III ACNGT 1 cut(s) 735
HpyCH4IV ACGT 1 cut(s) 1054
HpyCH4V TGCA 3 cut(s) 635, 965, 1115
HpyF10VI GCNNNNNNNGC 4 cut(s) 177, 667, 676, 1094
HpyF3I CTNAG 3 cut(s) 5, 29, 651
HpySE526I ACGT 1 cut(s) 1054
Hsp92II CATG 3 cut(s) 703, 1076, 1096
Kzo9I GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
LmnI GCTCC 4 cut(s) 666, 871, 993, 1104
Lsp1109I GCAGC 3 cut(s) 303, 775, 1100
LweI GCATC 7 cut(s) 274, 292, 526, 637, 785, 952, 1008
MaeI CTAG 4 cut(s) 755, 996, 1085, 1166
MaeII ACGT 1 cut(s) 1054
MaeIII GTNAC 1 cut(s) 567
MalI GATC 6 cut(s) 369, 492, 753, 787, 812, 1134
MboI GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
MflI RGATCY 3 cut(s) 751, 785, 1132
MluCI AATT 8 cut(s) 233, 425, 452, 503, 560, 711, 889, 1020
MlyI GAGTC 4 cut(s) 53, 110, 263, 619
MmeI TCCRAC 2 cut(s) 429, 806
MroXI GAANNNNTTC 1 cut(s) 1020
MseI TTAA 4 cut(s) 458, 522, 857, 1109
MspA1I CMGCKG 1 cut(s) 500
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 4 cut(s) 109, 868, 1037, 1181
MvaI CCWGG 3 cut(s) 868, 1037, 1181
MwoI GCNNNNNNNGC 4 cut(s) 177, 667, 676, 1094
NciI CCSGG 1 cut(s) 109
NcoI CCATGG 1 cut(s) 1092
NdeI CATATG 2 cut(s) 312, 919
NdeII GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
NheI GCTAGC 1 cut(s) 1084
NlaIII CATG 3 cut(s) 703, 1076, 1096
NlaIV GGNNCC 2 cut(s) 191, 989
NmuCI GTSAC 1 cut(s) 567
NspV TTCGAA 1 cut(s) 715
PceI AGGCCT 1 cut(s) 592
PdmI GAANNNNTTC 1 cut(s) 1020
PfeI GAWTC 1 cut(s) 695
PflFI GACNNNGTC 1 cut(s) 628
PfoI TCCNGGA 2 cut(s) 107, 1179
PkrI GCNGC 3 cut(s) 318, 765, 1090
PleI GAGTC 4 cut(s) 53, 109, 262, 619
PpsI GAGTC 4 cut(s) 53, 109, 262, 619
Psp6I CCWGG 3 cut(s) 866, 1035, 1179
PspGI CCWGG 3 cut(s) 866, 1035, 1179
PspN4I GGNNCC 2 cut(s) 191, 989
PspPI GGNCC 1 cut(s) 218
PstI CTGCAG 1 cut(s) 637
PsuI RGATCY 3 cut(s) 751, 785, 1132
PsyI GACNNNGTC 1 cut(s) 628
RsaI GTAC 1 cut(s) 1163
RsaNI GTAC 1 cut(s) 1162
SaqAI TTAA 4 cut(s) 458, 522, 857, 1109
SatI GCNGC 3 cut(s) 317, 764, 1089
Sau3AI GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 4 cut(s) 53, 110, 263, 619
ScrFI CCNGG 4 cut(s) 109, 868, 1037, 1181
SfaNI GCATC 7 cut(s) 274, 292, 526, 637, 785, 952, 1008
SfcI CTRYAG 1 cut(s) 633
SfuI TTCGAA 1 cut(s) 715
SinI GGWCC 1 cut(s) 218
SmlI CTYRAG 3 cut(s) 395, 512, 1136
SmoI CTYRAG 3 cut(s) 395, 512, 1136
Sse9I AATT 8 cut(s) 233, 425, 452, 503, 560, 711, 889, 1020
SseBI AGGCCT 1 cut(s) 592
SsiI CCGC 3 cut(s) 126, 201, 498
SspMI CTAG 4 cut(s) 755, 996, 1085, 1166
StuI AGGCCT 1 cut(s) 592
StyD4I CCNGG 4 cut(s) 107, 866, 1035, 1179
StyI CCWWGG 2 cut(s) 553, 1092
TaaI ACNGT 1 cut(s) 735
TaiI ACGT 1 cut(s) 1057
TaqI TCGA 5 cut(s) 132, 273, 489, 715, 1057
TaqII GACCGA 1 cut(s) 235
TasI AATT 8 cut(s) 233, 425, 452, 503, 560, 711, 889, 1020
TfiI GAWTC 1 cut(s) 695
Tru1I TTAA 4 cut(s) 458, 522, 857, 1109
Tru9I TTAA 4 cut(s) 458, 522, 857, 1109
TseFI GTSAC 1 cut(s) 567
TseI GCWGC 3 cut(s) 316, 763, 1088
Tsp45I GTSAC 1 cut(s) 567
TspDTI ATGAA 4 cut(s) 246, 345, 362, 1089
TspGWI ACGGA 1 cut(s) 1117
Tth111I GACNNNGTC 1 cut(s) 628
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 3 cut(s) 233, 503, 1020
XbaI TCTAGA 1 cut(s) 754
XmnI GAANNNNTTC 1 cut(s) 1020
XspI CTAG 4 cut(s) 755, 996, 1085, 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.