Rorug05G0443900

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002738
Physical Location & Seq
Forward (+)
61510189 .. 61513299
3111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0443900.1

Sequence Viewer

Length: 3111 bp
ATGTCAACAGAGGAGGATGGGAAGAAGAAGTTTACCAAGAGAGACGAGCTCCTCAAAATCGAAAAAAATGTTCAAAGCTGGTGGGAAGAGGAGCGAATTTTCGAGGCTAAAGCTCGTAAAAACTCTCCTAAACCAGGAGAAAAGTTCTTTGGGACTTTCCCTTTCCCTTACATGAATGGCGTTCTCCATCTTGGGCATGCATTCTCACTTTCAAAGCTCGAGTTTGCCGCAGCTTATCACAGATTAAGAGGTGCCAATGTGCTCTTGCCTTTTGCTTTCCACTGCACTGGCATGCCCATCAAAGCGTCTGCCGATAAACTTGCAAGAGAGGTTCAAGAGTTTGGTTGTCCTCCAGTGTTTCCCATTAATGCAGAGCCGGGGAAACAGGAAGAAAAAAGAAAAGGTATGAGTGCAGGTTCACTTCCGGGTAAGAAGCACAAGGGAAAGAAGTCTAAGGCTGTGTTGAAATCAGCCGGAGAAGCATACCAATGGTTTATTATGCGCAAGGGTACTGACCTGCCTGACAGTGAGATAGAGAAGTTCCAAGACCCAAAACAGTGGTTGACATACTTTCCTCCGAAGGCAGTGAAAGACCTCAAGGATTTTGGCTTGGGCTGTGACTGGAGGCGCTCCTTTATAACCACAGACATGAACCCTTTCTATGACGCCCTTGTGAAGTGGCAGGTTAGGAAACTCAAATCCATGGGTAAGATTGTGAAAGATGTCCGGTATACAATATACTCTCCAAAAGATGGACAGCCATGTGCAGATCATGATAGGGCCAGCGGCGAAGGAGTTCAGCCACAAGAGTACACTGTGATTAAAATGGAGGTGGTGGCACCTTTTCCTTCAAAGTTGGGAGTGTTGGAGGGGAGGAATGTGTTCCTTGCTGCCACAATGTTGAGACCTGAGACAATCTATGGACAAACAAATGCATGGATTTTGCCTGATGGGAAGTATGGAGCCTTTGAAATCAATGAAGCAGATGTCTTTATTCTTACTAGGAGAGCGGCACTCAATCTTGCCTATCAGAAGTACTCTAGGGTCGTAGAGAAGCCTACTTGCTTGGTTGAAATAAATGGTAACGACTTGATAGGCCTTCCATTGAAGTCTCCACTTGCACCGAATCAGATAGTTTATGCACTTCCCATATTGACCATCCTAGCAGACAATGGTACTGGGATAGTGACCAGCTTGCCTAGTGATGCTCCTGAAATGATGGCCTGGCGCGATTTAAAATCAAAACCTGCTTTGAGGGAAAAATATGGTGTGAGGGAAGAATGGATCATGCCCTTTGAAGGAAGGAAAGTTGAGGAGGCTGAACCACTAACAAGGAGCAAACTTATAGAGGCCAGAGAAGCATTCATCTATAGTGAACCTGAGCAGCGAGTAGTGTCAAGGTCCGGTGACGAATGCATTGTGGCGCTTACAGATCAATGGTACATCACATATGGAGAAGCTGAATGGAAAAAATTGGCTGAGGAGTGCTTATCCGGCATGAGTTTGTATTCTGATGAGACAAGGCGTGGATTTGAACACACATTGGGGTGGTTGAATCAGTGGGCTTGTTCACGATCTTTCGGACTTGGGACTCGTATCCCATGGGATGAAGAATTCTTAGTTGAGTCTTTGTCGGATTCAACTATTTATATGGCTTATTACACCATTGCTCACTTCTTACACAATGGAAATATGTATGTTGGCAGCAACTCAGAAGTTAGACCTGAACATATGACTGATGAGGTTTGGGATTTCATTTTTTGTGAAGGTCCAAAACCTAAATATTCGGTATTGATACATCAATTCTCTTCAGACCGAAAGAAACAACAAAATACATCAATTCTTGATGAGATGAAGCAAGAGTTTCAGTATTGGTATCCCTTTGATCTTCGAGTGTCCGGAAAGGATTTGATTCAAAATCACCTAACCTTCTGTATATATAATCATGCAGCCATTATGGCCAAGAAGCACTGGCCGCGCGGGTTTAGGTGCAATGGGCACCTAATGCTCAATGCCGAAAAGATGTCCAAGGCCAAAGGGAACTTCAAGACACTTGCCGAGGCAATAAACGAATTTTCTGCTGATGCCACACGATTCTCTTTGGCTGATGCAGGGGATGGTGTTGAAAACGCAAACTTTGACTCTGAGACTGCAAATGCTGCAATCTTGCGGCTCAAGAAAGAGATGGACTGGATGAAAGAGGTCTTGAAACAAGAGCATCATTTGAGAACGGGTCCACCATCTACATTTGCTGACAGAGTCTTTGCAAATGAGATTAATATTGCTGTCAACAGAACTGAGAAGAATTACCAGGCTTACATGTTCCGAGAAGCGCTTAAGACCGGGGTGTATGATCTTCAAGCTGCAAGGGATGAGTATAGGTTTTCATGTGGCACTTTGGAGGGCATGAACCGTGATTTGGTTTGGCGTTTTATGGATGTGCAGACGCGTCTTATTACTCCAATATGTCCACACTACGCAGAACATGTCTGGAGAAAAATTTTGAACAAGGAAGGGTTTGTGGTAAAAGCAGGATGGCCTGTGGCTGATGCTCTAGATATAACCCTCCAAAGAGCCAATAAGTACTTGCAAGCGTCCATTCTTTTAATCAGAAAGCTGTTTCACAAGAAAAGTTCCAAGAAGGTGGCAGATGATCACAGGAAACAGCTCAGAGGCTTGATTTATGTAAACGAACAATTTGATGGGTGGCAGGCGAAGTGCTTGACGATACTCAAGAGTAACTTTGACTCCGATAATCGCAGTTTTTCTTCAGACATTGACAAGGTGATACAGGAGGCATTGCCTACAATTTGTGTTGGTGAGTTGAAAGATTTGAAGGAAAAGCTAAAAGAGTGTATGCCTTTCATAAAGTTGAAGAAGAATGAGGCAATTGCACTTGGGGCTCAGGCCTTGGAATTGAGGCTACCGTTTGGAGAGATTGAGTTCCTTCAAGACAACTTGGATTGGATAAAGAGACAAGTTGGTCTTGAAGAGGTTCAAGTTTTATCGGCCTCCAATCATGATGATAGAGCTTTAGCTGGTTCTCATGCTGAACAGATGGAGCAGAATCCTCCAGTCCCTGGAAGGCCAACTGTCATCTTCATTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF812

Protein Analysis

1036

Amino Acids

118.47

Weight (kDa)

7.95

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1 PF00133 24 - 107 2.4e-09 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1 PF00133 179 - 713 1.9e-15 tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g PF09334 619 - 718 3e-07 tRNA synthetases class I (M)
tRNA-synt_1e PF01406 626 - 703 6.2e-07 tRNA synthetases class I (C) catalytic domain
Anticodon_1 PF08264 752 - 883 6.2e-15 Anticodon-binding domain of tRNA ligase
RBD_LARS1 PF24810 897 - 975 3.3e-07 Leucine--tRNA ligase, RagD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
AasI GACNNNNNNGTC 1 cut(s) 2982
Acc16I TGCGCA 1 cut(s) 503
Acc36I ACCTGC 4 cut(s) 404, 525, 673, 1255
AccB1I GGYRCC 3 cut(s) 251, 838, 1998
AccB7I CCANNNNNTGG 2 cut(s) 752, 3080
AccBSI CCGCTC 1 cut(s) 1010
AccI GTMKAC 1 cut(s) 731
AccII CGCG 4 cut(s) 1230, 1978, 1980, 2449
AccIII TCCGGA 1 cut(s) 1898
AciI CCGC 6 cut(s) 228, 786, 1010, 1976, 1980, 2170
AclWI GGATC 1 cut(s) 1292
AcoI YGGCCR 2 cut(s) 1959, 1973
AcsI RAATTY 4 cut(s) 96, 1613, 2072, 2499
AcuI CTGAAG 2 cut(s) 1794, 2754
AcyI GRCGYC 1 cut(s) 666
AfaI GTAC 6 cut(s) 511, 812, 1037, 1177, 1442, 2585
AfeI AGCGCT 1 cut(s) 2334
AfiI CCNNNNNNNGG 5 cut(s) 134, 752, 1298, 2419, 3080
AflII CTTAAG 1 cut(s) 2336
AflIII ACRYGT 3 cut(s) 2319, 2447, 2485
AjnI CCWGG 4 cut(s) 133, 1223, 2310, 3079
AjuI GAANNNNNNNTTGG 6 cut(s) 132, 164, 2027, 2059, 2402, 2434
Alw21I GWGCWC 2 cut(s) 51, 264
Alw26I GTCTC 7 cut(s) 36, 898, 905, 1116, 1511, 2141, 2968
AlwI GGATC 1 cut(s) 1292
AlwNI CAGNNNCTG 1 cut(s) 3080
Ama87I CYCGRG 1 cut(s) 218
Aor13HI TCCGGA 1 cut(s) 1898
Aor51HI AGCGCT 1 cut(s) 2334
ApeKI GCWGC 7 cut(s) 230, 890, 1384, 1704, 1949, 2159, 2363
ApoI RAATTY 4 cut(s) 96, 1613, 2072, 2499
ArsI GACNNNNNNTTYG 2 cut(s) 2246, 2278
AseI ATTAAT 2 cut(s) 366, 2277
Asp700I GAANNNNTTC 4 cut(s) 656, 795, 881, 2994
AspLEI GCGC 6 cut(s) 504, 630, 1230, 1426, 1980, 2335
AspS9I GGNCC 4 cut(s) 780, 1401, 1769, 2234
AsuC2I CCSGG 3 cut(s) 378, 426, 2344
AsuHPI GGTGA 4 cut(s) 1418, 1913, 2797, 2831
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 3 cut(s) 1401, 1769, 2234
BaeGI GKGCMC 1 cut(s) 2001
BalI TGGCCA 1 cut(s) 1961
BanI GGYRCC 3 cut(s) 251, 838, 1998
BanII GRGCYC 2 cut(s) 51, 2905
Bbv12I GWGCWC 2 cut(s) 51, 264
BbvCI CCTCAGC 1 cut(s) 1479
BbvI GCAGC 7 cut(s) 242, 877, 1396, 1716, 1961, 2146, 2350
BcgI CGANNNNNNTGC 4 cut(s) 302, 336, 2060, 2094
BciT130I CCWGG 4 cut(s) 135, 1225, 2312, 3081
BciVI GTATCC 2 cut(s) 1607, 1887
BclI TGATCA 1 cut(s) 2653
BcnI CCSGG 3 cut(s) 378, 426, 2344
BcoDI GTCTC 7 cut(s) 36, 898, 905, 1116, 1511, 2141, 2968
BfaI CTAG 5 cut(s) 1002, 1041, 1163, 1200, 2555
BfmI CTRYAG 1 cut(s) 1369
BfoI RGCGCY 3 cut(s) 631, 1427, 2336
BfrI CTTAAG 1 cut(s) 2336
BfuAI ACCTGC 4 cut(s) 404, 525, 673, 1255
BfuI GTATCC 2 cut(s) 1607, 1887
BglI GCCNNNNNGGC 1 cut(s) 1958
BmcAI AGTACT 2 cut(s) 1037, 2585
Bme1390I CCNGG 7 cut(s) 135, 378, 426, 1225, 2312, 2344, 3081
Bme18I GGWCC 3 cut(s) 1401, 1769, 2234
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 4 cut(s) 780, 1401, 1769, 2234
BmiI GGNNCC 5 cut(s) 253, 840, 964, 2000, 2235
BmrFI CCNGG 7 cut(s) 135, 378, 426, 1225, 2312, 2344, 3081
BmrI ACTGGG 1 cut(s) 1188
BmsI GCATC 5 cut(s) 1195, 2074, 2098, 2227, 2539
BmuI ACTGGG 1 cut(s) 1188
BplI GAGNNNNNCTC 4 cut(s) 33, 65, 999, 1031
BpmI CTGGAG 4 cut(s) 336, 643, 2512, 3057
Bpu10I CCTNAGC 3 cut(s) 1380, 1479, 2904
BpuEI CTTGAG 3 cut(s) 581, 2159, 2717
BpuMI CCSGG 3 cut(s) 378, 426, 2344
BsaHI GRCGYC 1 cut(s) 666
BsaI GGTCTC 1 cut(s) 898
BsaJI CCNNGG 8 cut(s) 377, 702, 1601, 2028, 2058, 2343, 2910, 3079
BsaWI WCCGGW 3 cut(s) 726, 1403, 1898
BsaXI ACNNNNNCTCC 2 cut(s) 2732, 2762
Bsc4I CCNNNNNNNGG 5 cut(s) 134, 752, 1298, 2419, 3080
Bse1I ACTGG 7 cut(s) 292, 353, 626, 1183, 1976, 2195, 3074
Bse3DI GCAATG 3 cut(s) 1665, 1999, 2798
BseAI TCCGGA 1 cut(s) 1898
BseBI CCWGG 4 cut(s) 135, 1225, 2312, 3081
BseDI CCNNGG 8 cut(s) 377, 702, 1601, 2028, 2058, 2343, 2910, 3079
BseGI GGATG 8 cut(s) 22, 1158, 1612, 2122, 2199, 2377, 2443, 2540
BseLI CCNNNNNNNGG 5 cut(s) 134, 752, 1298, 2419, 3080
BseMI GCAATG 3 cut(s) 1665, 1999, 2798
BseMII CTCAG 8 cut(s) 900, 1371, 1470, 1725, 2136, 2289, 2683, 2918
BseNI ACTGG 7 cut(s) 292, 353, 626, 1183, 1976, 2195, 3074
BseRI GAGGAG 5 cut(s) 26, 41, 104, 1328, 1496
BseSI GKGCMC 1 cut(s) 2001
BseXI GCAGC 7 cut(s) 242, 877, 1396, 1716, 1961, 2146, 2350
BsgI GTGCAG 4 cut(s) 268, 432, 786, 2462
Bsh1236I CGCG 4 cut(s) 1230, 1978, 1980, 2449
BshNI GGYRCC 3 cut(s) 251, 838, 1998
BsiHKAI GWGCWC 2 cut(s) 51, 264
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 8 cut(s) 377, 425, 474, 727, 1404, 1494, 1899, 2343
BslFI GGGAC 3 cut(s) 166, 1603, 3062
BslI CCNNNNNNNGG 5 cut(s) 134, 752, 1298, 2419, 3080
BsmAI GTCTC 7 cut(s) 36, 898, 905, 1116, 1511, 2141, 2968
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 3 cut(s) 166, 1603, 3062
BsmI GAATGC 3 cut(s) 200, 1361, 1418
Bso31I GGTCTC 1 cut(s) 898
BsoBI CYCGRG 1 cut(s) 218
Bsp1286I GDGCHC 4 cut(s) 51, 264, 2001, 2905
Bsp13I TCCGGA 1 cut(s) 1898
Bsp143I GATC 7 cut(s) 769, 1284, 1432, 1574, 1885, 2353, 2653
Bsp19I CCATGG 2 cut(s) 702, 1601
BspACI CCGC 6 cut(s) 228, 786, 1010, 1976, 1980, 2170
BspCNI CTCAG 8 cut(s) 901, 1372, 1471, 1724, 2137, 2290, 2682, 2917
BspEI TCCGGA 1 cut(s) 1898
BspFNI CGCG 4 cut(s) 1230, 1978, 1980, 2449
BspHI TCATGA 2 cut(s) 772, 3019
BspLI GGNNCC 5 cut(s) 253, 840, 964, 2000, 2235
BspMI ACCTGC 4 cut(s) 404, 525, 673, 1255
BspPI GGATC 1 cut(s) 1292
BspT107I GGYRCC 3 cut(s) 251, 838, 1998
BspTI CTTAAG 1 cut(s) 2336
BspTNI GGTCTC 1 cut(s) 898
BsrBI CCGCTC 1 cut(s) 1010
BsrDI GCAATG 3 cut(s) 1665, 1999, 2798
BsrI ACTGG 7 cut(s) 292, 353, 626, 1183, 1976, 2195, 3074
BssECI CCNNGG 8 cut(s) 377, 702, 1601, 2028, 2058, 2343, 2910, 3079
BssMI GATC 7 cut(s) 769, 1284, 1432, 1574, 1885, 2353, 2653
BssNAI GTATAC 1 cut(s) 732
BssNI GRCGYC 1 cut(s) 666
BssT1I CCWWGG 4 cut(s) 702, 1601, 2028, 2910
Bst1107I GTATAC 1 cut(s) 732
Bst2UI CCWGG 4 cut(s) 135, 1225, 2312, 3081
Bst4CI ACNGT 6 cut(s) 527, 558, 817, 2414, 2928, 3094
Bst6I CTCTTC 3 cut(s) 81, 1813, 2985
BstACI GRCGYC 1 cut(s) 666
BstAFI CTTAAG 1 cut(s) 2336
BstAPI GCANNNNNTGC 2 cut(s) 2005, 2159
BstC8I GCNNGC 6 cut(s) 198, 293, 784, 1196, 2592, 2712
BstDSI CCRYGG 2 cut(s) 702, 1601
BstENI CCTNNNNNAGG 1 cut(s) 132
BstF5I GGATG 8 cut(s) 22, 1158, 1612, 2122, 2199, 2377, 2443, 2540
BstFNI CGCG 4 cut(s) 1230, 1978, 1980, 2449
BstH2I RGCGCY 3 cut(s) 631, 1427, 2336
BstHHI GCGC 6 cut(s) 504, 630, 1230, 1426, 1980, 2335
BstKTI GATC 7 cut(s) 772, 1287, 1435, 1577, 1888, 2356, 2656
BstMAI GTCTC 7 cut(s) 36, 898, 905, 1116, 1511, 2141, 2968
BstMBI GATC 7 cut(s) 769, 1284, 1432, 1574, 1885, 2353, 2653
BstMWI GCNNNNNNNGC 8 cut(s) 479, 1358, 1494, 1958, 1975, 2005, 2159, 2900
BstNI CCWGG 4 cut(s) 135, 1225, 2312, 3081
BstNSI RCATGY 4 cut(s) 200, 295, 2323, 2489
BstSCI CCNGG 7 cut(s) 133, 376, 424, 1223, 2310, 2342, 3079
BstSFI CTRYAG 1 cut(s) 1369
BstSLI GKGCMC 1 cut(s) 2001
BstUI CGCG 4 cut(s) 1230, 1978, 1980, 2449
BstV1I GCAGC 7 cut(s) 242, 877, 1396, 1716, 1961, 2146, 2350
BstXI CCANNNNNNTGG 3 cut(s) 287, 558, 2644
BstZ17I GTATAC 1 cut(s) 732
BsuI GTATCC 2 cut(s) 1607, 1887
BtgI CCRYGG 2 cut(s) 702, 1601
BtsCI GGATG 8 cut(s) 22, 1158, 1612, 2122, 2199, 2377, 2443, 2540
BtsI GCAGTG 2 cut(s) 280, 591
BtsIMutI CAGTG 9 cut(s) 280, 285, 360, 532, 563, 591, 813, 1565, 1969
BveI ACCTGC 4 cut(s) 404, 525, 673, 1255
Cac8I GCNNGC 6 cut(s) 198, 293, 784, 1196, 2592, 2712
CaiI CAGNNNCTG 1 cut(s) 3080
CciI TCATGA 2 cut(s) 772, 3019
CfoI GCGC 6 cut(s) 504, 630, 1230, 1426, 1980, 2335
Cfr13I GGNCC 4 cut(s) 780, 1401, 1769, 2234
CseI GACGC 5 cut(s) 294, 674, 2438, 2455, 2583
Csp6I GTAC 6 cut(s) 510, 811, 1036, 1176, 1441, 2584
CspCI CAANNNNNGTGG 2 cut(s) 62, 97
CviQI GTAC 6 cut(s) 510, 811, 1036, 1176, 1441, 2584
DpnI GATC 7 cut(s) 771, 1286, 1434, 1576, 1887, 2355, 2655
DpnII GATC 7 cut(s) 769, 1284, 1432, 1574, 1885, 2353, 2653
DraI TTTAAA 1 cut(s) 1236
DrdI GACNNNNNNGTC 1 cut(s) 2982
DseDI GACNNNNNNGTC 1 cut(s) 2982
EaeI YGGCCR 2 cut(s) 1959, 1973
Eam1104I CTCTTC 3 cut(s) 81, 1813, 2985
EarI CTCTTC 3 cut(s) 81, 1813, 2985
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 4 cut(s) 702, 1601, 2028, 2910
Eco147I AGGCCT 2 cut(s) 1098, 2909
Eco24I GRGCYC 2 cut(s) 51, 2905
Eco31I GGTCTC 1 cut(s) 898
Eco47I GGWCC 3 cut(s) 1401, 1769, 2234
Eco47III AGCGCT 1 cut(s) 2334
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 2 cut(s) 1794, 2754
Eco88I CYCGRG 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 49
EcoNI CCTNNNNNAGG 1 cut(s) 132
EcoRI GAATTC 1 cut(s) 1613
EcoRII CCWGG 4 cut(s) 133, 1223, 2310, 3079
EcoT14I CCWWGG 4 cut(s) 702, 1601, 2028, 2910
EcoT22I ATGCAT 3 cut(s) 202, 937, 1418
EcoT38I GRGCYC 2 cut(s) 51, 2905
ErhI CCWWGG 4 cut(s) 702, 1601, 2028, 2910
Esp3I CGTCTC 1 cut(s) 36
FalI AAGNNNNNCTT 4 cut(s) 2726, 2758, 2970, 3002
FaqI GGGAC 3 cut(s) 166, 1603, 3062
FauI CCCGC 1 cut(s) 1973
FauNDI CATATG 2 cut(s) 1450, 1731
FbaI TGATCA 1 cut(s) 2653
FblI GTMKAC 1 cut(s) 731
FokI GGATG 8 cut(s) 29, 1145, 1619, 2129, 2206, 2384, 2450, 2547
FriOI GRGCYC 2 cut(s) 51, 2905
FspBI CTAG 5 cut(s) 1002, 1041, 1163, 1200, 2555
FspI TGCGCA 1 cut(s) 503
GlaI GCGC 6 cut(s) 503, 629, 1229, 1425, 1979, 2334
GsuI CTGGAG 4 cut(s) 336, 643, 2512, 3057
HaeII RGCGCY 3 cut(s) 631, 1427, 2336
HapII CCGG 8 cut(s) 377, 425, 474, 727, 1404, 1494, 1899, 2343
HgaI GACGC 5 cut(s) 294, 674, 2438, 2455, 2583
HhaI GCGC 6 cut(s) 504, 630, 1230, 1426, 1980, 2335
Hin1I GRCGYC 1 cut(s) 666
Hin6I GCGC 6 cut(s) 502, 628, 1228, 1424, 1978, 2333
HinP1I GCGC 6 cut(s) 502, 628, 1228, 1424, 1978, 2333
HincII GTYRAC 3 cut(s) 6, 564, 2290
HindII GTYRAC 3 cut(s) 6, 564, 2290
HpaII CCGG 8 cut(s) 377, 425, 474, 727, 1404, 1494, 1899, 2343
HphI GGTGA 4 cut(s) 1418, 1913, 2797, 2831
HpyCH4III ACNGT 6 cut(s) 527, 558, 817, 2414, 2928, 3094
HpyF10VI GCNNNNNNNGC 8 cut(s) 479, 1358, 1494, 1958, 1975, 2005, 2159, 2900
Hsp92I GRCGYC 1 cut(s) 666
HspAI GCGC 6 cut(s) 502, 628, 1228, 1424, 1978, 2333
Kpn2I TCCGGA 1 cut(s) 1898
Ksp22I TGATCA 1 cut(s) 2653
Kzo9I GATC 7 cut(s) 769, 1284, 1432, 1574, 1885, 2353, 2653
LmnI GCTCC 7 cut(s) 54, 91, 635, 962, 1213, 1335, 3061
Lsp1109I GCAGC 7 cut(s) 242, 877, 1396, 1716, 1961, 2146, 2350
LweI GCATC 5 cut(s) 1195, 2074, 2098, 2227, 2539
MaeI CTAG 5 cut(s) 1002, 1041, 1163, 1200, 2555
MaeIII GTNAC 5 cut(s) 617, 1082, 1186, 1406, 2738
MalI GATC 7 cut(s) 771, 1286, 1434, 1576, 1887, 2355, 2655
MbiI CCGCTC 1 cut(s) 1010
MboI GATC 7 cut(s) 769, 1284, 1432, 1574, 1885, 2353, 2653
MfeI CAATTG 1 cut(s) 2889
MhlI GDGCHC 4 cut(s) 51, 264, 2001, 2905
MlsI TGGCCA 1 cut(s) 1961
MluI ACGCGT 1 cut(s) 2447
MluNI TGGCCA 1 cut(s) 1961
MlyI GAGTC 5 cut(s) 1585, 1634, 2135, 2268, 2741
MmeI TCCRAC 2 cut(s) 846, 1614
Mox20I TGGCCA 1 cut(s) 1961
Mph1103I ATGCAT 3 cut(s) 202, 937, 1418
MroI TCCGGA 1 cut(s) 1898
MroXI GAANNNNTTC 4 cut(s) 656, 795, 881, 2994
MscI TGGCCA 1 cut(s) 1961
MseI TTAA 7 cut(s) 245, 366, 822, 1235, 2277, 2337, 2606
MslI CAYNNNNRTG 4 cut(s) 290, 487, 647, 1546
Msp20I TGGCCA 1 cut(s) 1961
MspA1I CMGCKG 1 cut(s) 786
MspCI CTTAAG 1 cut(s) 2336
MspI CCGG 8 cut(s) 377, 425, 474, 727, 1404, 1494, 1899, 2343
MspR9I CCNGG 7 cut(s) 135, 378, 426, 1225, 2312, 2344, 3081
MunI CAATTG 1 cut(s) 2889
Mva1269I GAATGC 3 cut(s) 200, 1361, 1418
MvaI CCWGG 4 cut(s) 135, 1225, 2312, 3081
MvnI CGCG 4 cut(s) 1230, 1978, 1980, 2449
MwoI GCNNNNNNNGC 8 cut(s) 479, 1358, 1494, 1958, 1975, 2005, 2159, 2900
NciI CCSGG 3 cut(s) 378, 426, 2344
NcoI CCATGG 2 cut(s) 702, 1601
NdeI CATATG 2 cut(s) 1450, 1731
NdeII GATC 7 cut(s) 769, 1284, 1432, 1574, 1885, 2353, 2653
NlaIV GGNNCC 5 cut(s) 253, 840, 964, 2000, 2235
NmeAIII GCCGAG 1 cut(s) 2083
NmuCI GTSAC 3 cut(s) 617, 1186, 1406
NsbI TGCGCA 1 cut(s) 503
NsiI ATGCAT 3 cut(s) 202, 937, 1418
NspI RCATGY 4 cut(s) 200, 295, 2323, 2489
PaeI GCATGC 2 cut(s) 200, 295
PaeR7I CTCGAG 1 cut(s) 218
PagI TCATGA 2 cut(s) 772, 3019
PceI AGGCCT 2 cut(s) 1098, 2909
PciI ACATGT 2 cut(s) 2319, 2485
PctI GAATGC 3 cut(s) 200, 1361, 1418
PdmI GAANNNNTTC 4 cut(s) 656, 795, 881, 2994
PfeI GAWTC 6 cut(s) 1126, 1555, 1637, 1912, 2094, 3067
PflFI GACNNNGTC 1 cut(s) 2258
PflMI CCANNNNNTGG 2 cut(s) 752, 3080
PleI GAGTC 5 cut(s) 1585, 1633, 2135, 2267, 2741
PpsI GAGTC 5 cut(s) 1585, 1633, 2135, 2267, 2741
PscI ACATGT 2 cut(s) 2319, 2485
PshBI ATTAAT 2 cut(s) 366, 2277
PsiI TTATAA 1 cut(s) 638
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 4 cut(s) 133, 1223, 2310, 3079
PspGI CCWGG 4 cut(s) 133, 1223, 2310, 3079
PspN4I GGNNCC 5 cut(s) 253, 840, 964, 2000, 2235
PspPI GGNCC 4 cut(s) 780, 1401, 1769, 2234
PspXI VCTCGAGB 1 cut(s) 218
PstNI CAGNNNCTG 1 cut(s) 3080
PsyI GACNNNGTC 1 cut(s) 2258
RsaI GTAC 6 cut(s) 511, 812, 1037, 1177, 1442, 2585
RsaNI GTAC 6 cut(s) 510, 811, 1036, 1176, 1441, 2584
RseI CAYNNNNRTG 4 cut(s) 290, 487, 647, 1546
SacI GAGCTC 1 cut(s) 51
SaqAI TTAA 7 cut(s) 245, 366, 822, 1235, 2277, 2337, 2606
Sau3AI GATC 7 cut(s) 769, 1284, 1432, 1574, 1885, 2353, 2653
Sau96I GGNCC 4 cut(s) 780, 1401, 1769, 2234
ScaI AGTACT 2 cut(s) 1037, 2585
SchI GAGTC 5 cut(s) 1585, 1634, 2135, 2268, 2741
ScrFI CCNGG 7 cut(s) 135, 378, 426, 1225, 2312, 2344, 3081
SduI GDGCHC 4 cut(s) 51, 264, 2001, 2905
SfaNI GCATC 5 cut(s) 1195, 2074, 2098, 2227, 2539
SfcI CTRYAG 1 cut(s) 1369
Sfr274I CTCGAG 1 cut(s) 218
SinI GGWCC 3 cut(s) 1401, 1769, 2234
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 4 cut(s) 290, 487, 647, 1546
SmlI CTYRAG 5 cut(s) 218, 596, 2174, 2336, 2732
SmoI CTYRAG 5 cut(s) 218, 596, 2174, 2336, 2732
SphI GCATGC 2 cut(s) 200, 295
SseBI AGGCCT 2 cut(s) 1098, 2909
SsiI CCGC 6 cut(s) 228, 786, 1010, 1976, 1980, 2170
SspI AATATT 2 cut(s) 1784, 2281
SspMI CTAG 5 cut(s) 1002, 1041, 1163, 1200, 2555
SstI GAGCTC 1 cut(s) 51
StuI AGGCCT 2 cut(s) 1098, 2909
StyD4I CCNGG 7 cut(s) 133, 376, 424, 1223, 2310, 2342, 3079
StyI CCWWGG 4 cut(s) 702, 1601, 2028, 2910
TaaI ACNGT 6 cut(s) 527, 558, 817, 2414, 2928, 3094
TaqI TCGA 4 cut(s) 60, 102, 219, 1891
TaqII GACCGA 1 cut(s) 1830
TatI WGTACW 3 cut(s) 810, 1035, 2583
TauI GCSGC 5 cut(s) 230, 789, 1013, 1978, 2173
TfiI GAWTC 6 cut(s) 1126, 1555, 1637, 1912, 2094, 3067
Tru1I TTAA 7 cut(s) 245, 366, 822, 1235, 2277, 2337, 2606
Tru9I TTAA 7 cut(s) 245, 366, 822, 1235, 2277, 2337, 2606
TscAI CASTG 9 cut(s) 287, 292, 360, 532, 563, 591, 820, 1565, 1976
TseFI GTSAC 3 cut(s) 617, 1186, 1406
TseI GCWGC 7 cut(s) 230, 890, 1384, 1704, 1949, 2159, 2363
Tsp45I GTSAC 3 cut(s) 617, 1186, 1406
TspRI CASTG 9 cut(s) 287, 292, 360, 532, 563, 591, 820, 1565, 1976
Tth111I GACNNNGTC 1 cut(s) 2258
Van91I CCANNNNNTGG 2 cut(s) 752, 3080
Vha464I CTTAAG 1 cut(s) 2336
VpaK11BI GGWCC 3 cut(s) 1401, 1769, 2234
VspI ATTAAT 2 cut(s) 366, 2277
XagI CCTNNNNNAGG 1 cut(s) 132
XapI RAATTY 4 cut(s) 96, 1613, 2072, 2499
XbaI TCTAGA 1 cut(s) 2554
XceI RCATGY 4 cut(s) 200, 295, 2323, 2489
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 731
XmnI GAANNNNTTC 4 cut(s) 656, 795, 881, 2994
XspI CTAG 5 cut(s) 1002, 1041, 1163, 1200, 2555
ZrmI AGTACT 2 cut(s) 1037, 2585
Zsp2I ATGCAT 3 cut(s) 202, 937, 1418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.