Rh5DG534400

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002738
Physical Location & Seq
Forward (+)
82252282 .. 82255047
2766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG534400.1

Sequence Viewer

Length: 1191 bp
ATGCTTAGAAACAGACTATATGTGTTGCTTAGGCAGTTCTTGTATCCATCTGAAGAGGACTCGTGTAAGTTGGTAAGGTTCTTGGTGGAGAGGCTTTCGGAGTTGTCCGGGAGTGGAAGCAGGGGTGGTCTCAAGGGTGTTGAGGATATGGGGAGAGTGAAAGAAGATAGCTTTGAAAGCGATTTGGGAAACCACAGAAGCGGTGATGAAAGACTTGGACCGAAATGGGATGAATTCACATTGAATGGTGAAGAGCCTGAAGTATTGAATATCGAGGGTGAAGATGCCTCTGTTGGTTGTGATGCTAATCATATGCTGCGAAGATTGGATGAAATGAGTATTGACAATGTATCCAGCAGACCTGATGATCTTCATTCGTCAGAGGAGAAACAGCTCGAGGAGGAGGTGATGGCAAAGACTTCAGAATTGAAGCACCTTGAAGAAGAGTTGGAATTGTTAAAAGAAGCAGTAGATATAGCATTTGATGTCGATCATCCCGCTGAATTTTACCTCGAGAAACTTAATCAGCAAGTGGGTGCTCAAAGGCAACACCTAGGAGAATTGAAGTCACAGCAGGAAGTTGTCAGAAGGCCTTTGGAAGAGAAGAGGAGAACTCTTCAGGAGACTCTGTCTGCAGATAACCCAGATGCTCAGAAAAAACTCCAAAAGCTGAAACAGCTTGAAGTGGAAAAGGAATCCATGTTAGCTTCAATTCGAAAAAGGGAGGAGGAACTTTCTCAACTTACTATGGATCTAGAGAAGCAGCCCAAACTGACATCAAGGAGATCTTATATTGAAAGGGTCAAAGAGATCACAAAAAATAGTCGGAAGCAAGAAGCTGACATAGAGCAGATTTTAAAGGACACCAGGGAGCTTCAGTTAGAGAGTAATTCTATTCAAGAACGCCTCCATCGGACATATGCTGTTGTAGATGAGCTTGTTTTCAGGGAAGCAAAGAAAGATGCAGTGGGGAGGCAGGCTTATAGGCTCCTCACTAGCATCCACGAGAGCTTTGAACAAATTCGTGAGAAAATCCTGGCAACTGATAGATTACGTCGAGAAGCGACTGAACATGAAAAGAAGCTAGCAGCCATGGCTTCACGGAGTTTAAATGCAAACAAACTACAAGCAGATCTTGAGGTCATTAGGAGGGAGAATGAGTACCTAGAGAAATCTCTCGAGGATAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF812

Protein Analysis

396

Amino Acids

46.07

Weight (kDa)

5.22

Isoelectric Point (pI)

62.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC22_CC PF05667 127 - 364 2.8e-50 CCDC22 protein coiled-coil region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 201, 498
AclWI GGATC 1 cut(s) 759
AcsI RAATTY 3 cut(s) 233, 503, 1020
AcuI CTGAAG 5 cut(s) 72, 279, 405, 602, 860
AfaI GTAC 1 cut(s) 1163
AjnI CCWGG 2 cut(s) 866, 1035
Alw21I GWGCWC 1 cut(s) 541
Alw26I GTCTC 2 cut(s) 134, 617
AlwI GGATC 1 cut(s) 759
Ama87I CYCGRG 3 cut(s) 395, 512, 1178
AoxI GGCC 1 cut(s) 590
ApeKI GCWGC 3 cut(s) 316, 763, 1088
ApoI RAATTY 3 cut(s) 233, 503, 1020
Asp700I GAANNNNTTC 1 cut(s) 1020
AspA2I CCTAGG 1 cut(s) 553
AspS9I GGNCC 1 cut(s) 218
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 4 cut(s) 215, 260, 290, 418
AsuII TTCGAA 1 cut(s) 715
AsuNHI GCTAGC 1 cut(s) 1084
AvaI CYCGRG 3 cut(s) 395, 512, 1178
AvaII GGWCC 1 cut(s) 218
AvrII CCTAGG 1 cut(s) 553
BauI CACGAG 2 cut(s) 61, 1004
Bbv12I GWGCWC 1 cut(s) 541
BbvI GCAGC 3 cut(s) 303, 775, 1100
BccI CCATC 3 cut(s) 55, 403, 918
BciT130I CCWGG 2 cut(s) 868, 1037
BciVI GTATCC 2 cut(s) 54, 361
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 134, 617
BfaI CTAG 5 cut(s) 554, 755, 996, 1085, 1166
BfmI CTRYAG 1 cut(s) 633
BfuI GTATCC 2 cut(s) 54, 361
BglII AGATCT 2 cut(s) 785, 1132
BisI GCNGC 3 cut(s) 317, 764, 1089
BlnI CCTAGG 1 cut(s) 553
BlsI GCNGC 3 cut(s) 318, 765, 1090
Bme1390I CCNGG 3 cut(s) 109, 868, 1037
Bme18I GGWCC 1 cut(s) 218
BmeT110I CYCGRG 3 cut(s) 395, 512, 1178
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 989
BmrFI CCNGG 3 cut(s) 109, 868, 1037
BmsI GCATC 5 cut(s) 274, 292, 637, 952, 1008
BmtI GCTAGC 1 cut(s) 1088
BplI GAGNNNNNCTC 2 cut(s) 598, 630
Bpu10I CCTNAGC 1 cut(s) 29
Bpu14I TTCGAA 1 cut(s) 715
BpuEI CTTGAG 2 cut(s) 116, 1157
BpuMI CCSGG 1 cut(s) 109
BsaBI GATNNNNATC 2 cut(s) 306, 489
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 3 cut(s) 553, 867, 1092
BsaXI ACNNNNNCTCC 6 cut(s) 614, 644, 863, 893, 1145, 1175
Bse8I GATNNNNATC 2 cut(s) 306, 489
BseBI CCWGG 2 cut(s) 868, 1037
BseDI CCNNGG 3 cut(s) 553, 867, 1092
BseGI GGATG 4 cut(s) 235, 334, 493, 999
BseJI GATNNNNATC 2 cut(s) 306, 489
BseMII CTCAG 1 cut(s) 665
BseRI GAGGAG 6 cut(s) 398, 413, 416, 622, 740, 980
BseXI GCAGC 3 cut(s) 303, 775, 1100
BshFI GGCC 1 cut(s) 592
BsiHKAI GWGCWC 1 cut(s) 541
BsiHKCI CYCGRG 3 cut(s) 395, 512, 1178
BsiSI CCGG 1 cut(s) 108
BsmAI GTCTC 2 cut(s) 134, 617
BsnI GGCC 1 cut(s) 592
Bso31I GGTCTC 1 cut(s) 134
BsoBI CYCGRG 3 cut(s) 395, 512, 1178
Bsp119I TTCGAA 1 cut(s) 715
Bsp1286I GDGCHC 1 cut(s) 541
Bsp143I GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
Bsp19I CCATGG 1 cut(s) 1092
BspACI CCGC 2 cut(s) 201, 498
BspANI GGCC 1 cut(s) 592
BspCNI CTCAG 1 cut(s) 664
BspLI GGNNCC 1 cut(s) 989
BspMAI CTGCAG 1 cut(s) 637
BspOI GCTAGC 1 cut(s) 1088
BspPI GGATC 1 cut(s) 759
BspQI GCTCTTC 1 cut(s) 246
BspT104I TTCGAA 1 cut(s) 715
BspTNI GGTCTC 1 cut(s) 134
BssECI CCNNGG 3 cut(s) 553, 867, 1092
BssMI GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
BssSI CACGAG 2 cut(s) 61, 1004
BssT1I CCWWGG 2 cut(s) 553, 1092
Bst2BI CACGAG 2 cut(s) 61, 1004
Bst2UI CCWGG 2 cut(s) 868, 1037
Bst6I CTCTTC 6 cut(s) 48, 246, 438, 594, 599, 621
BstBI TTCGAA 1 cut(s) 715
BstC8I GCNNGC 2 cut(s) 978, 1086
BstDEI CTNAG 3 cut(s) 5, 29, 651
BstDSI CCRYGG 1 cut(s) 1092
BstF5I GGATG 4 cut(s) 235, 334, 493, 999
BstKTI GATC 6 cut(s) 370, 493, 754, 788, 813, 1135
BstMAI GTCTC 2 cut(s) 134, 617
BstMBI GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
BstMWI GCNNNNNNNGC 3 cut(s) 177, 676, 1094
BstNI CCWGG 2 cut(s) 868, 1037
BstSCI CCNGG 3 cut(s) 107, 866, 1035
BstSFI CTRYAG 1 cut(s) 633
BstV1I GCAGC 3 cut(s) 303, 775, 1100
BstX2I RGATCY 3 cut(s) 751, 785, 1132
BstYI RGATCY 3 cut(s) 751, 785, 1132
BsuI GTATCC 2 cut(s) 54, 361
BsuRI GGCC 1 cut(s) 592
BtgI CCRYGG 1 cut(s) 1092
BtsCI GGATG 4 cut(s) 235, 334, 493, 999
BtsI GCAGTG 1 cut(s) 972
BtsIMutI CAGTG 1 cut(s) 972
Cac8I GCNNGC 2 cut(s) 978, 1086
Cfr13I GGNCC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 1162
CviAII CATG 3 cut(s) 700, 1073, 1093
CviQI GTAC 1 cut(s) 1162
DdeI CTNAG 3 cut(s) 5, 29, 651
DpnI GATC 6 cut(s) 369, 492, 753, 787, 812, 1134
DpnII GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
DraI TTTAAA 2 cut(s) 858, 1110
Eam1104I CTCTTC 6 cut(s) 48, 246, 438, 594, 599, 621
EarI CTCTTC 6 cut(s) 48, 246, 438, 594, 599, 621
Eco130I CCWWGG 2 cut(s) 553, 1092
Eco147I AGGCCT 1 cut(s) 592
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 218
Eco57I CTGAAG 5 cut(s) 72, 279, 405, 602, 860
Eco88I CYCGRG 3 cut(s) 395, 512, 1178
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 866, 1035
EcoT14I CCWWGG 2 cut(s) 553, 1092
ErhI CCWWGG 2 cut(s) 553, 1092
FaeI CATG 3 cut(s) 703, 1076, 1096
FalI AAGNNNNNCTT 4 cut(s) 772, 804, 1119, 1151
FatI CATG 3 cut(s) 699, 1072, 1092
FauI CCCGC 1 cut(s) 505
FauNDI CATATG 2 cut(s) 312, 919
Fnu4HI GCNGC 3 cut(s) 317, 764, 1089
FokI GGATG 4 cut(s) 242, 341, 480, 986
Fsp4HI GCNGC 3 cut(s) 317, 764, 1089
FspBI CTAG 5 cut(s) 554, 755, 996, 1085, 1166
GluI GCNGC 3 cut(s) 317, 764, 1089
HaeIII GGCC 1 cut(s) 592
HapII CCGG 1 cut(s) 108
Hin1II CATG 3 cut(s) 703, 1076, 1096
HinfI GANTC 3 cut(s) 59, 625, 695
HpaII CCGG 1 cut(s) 108
HphI GGTGA 4 cut(s) 215, 260, 290, 418
Hpy188I TCNGA 8 cut(s) 52, 100, 382, 424, 587, 654, 828, 915
Hpy188III TCNNGA 8 cut(s) 514, 620, 755, 899, 1025, 1058, 1136, 1178
Hpy99I CGWCG 1 cut(s) 1059
HpyAV CCTTC 1 cut(s) 582
HpyCH4IV ACGT 1 cut(s) 1054
HpyCH4V TGCA 3 cut(s) 635, 965, 1115
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 676, 1094
HpyF3I CTNAG 3 cut(s) 5, 29, 651
HpySE526I ACGT 1 cut(s) 1054
Hsp92II CATG 3 cut(s) 703, 1076, 1096
Kzo9I GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 2 cut(s) 871, 993
Lsp1109I GCAGC 3 cut(s) 303, 775, 1100
LweI GCATC 5 cut(s) 274, 292, 637, 952, 1008
MaeI CTAG 5 cut(s) 554, 755, 996, 1085, 1166
MaeII ACGT 1 cut(s) 1054
MaeIII GTNAC 1 cut(s) 567
MalI GATC 6 cut(s) 369, 492, 753, 787, 812, 1134
MboI GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
MflI RGATCY 3 cut(s) 751, 785, 1132
MhlI GDGCHC 1 cut(s) 541
MluCI AATT 8 cut(s) 233, 425, 452, 503, 560, 711, 889, 1020
MlyI GAGTC 2 cut(s) 53, 619
MmeI TCCRAC 2 cut(s) 429, 806
MroXI GAANNNNTTC 1 cut(s) 1020
MseI TTAA 4 cut(s) 458, 522, 857, 1109
MspA1I CMGCKG 1 cut(s) 500
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 3 cut(s) 109, 868, 1037
MvaI CCWGG 2 cut(s) 868, 1037
MwoI GCNNNNNNNGC 3 cut(s) 177, 676, 1094
NciI CCSGG 1 cut(s) 109
NcoI CCATGG 1 cut(s) 1092
NdeI CATATG 2 cut(s) 312, 919
NdeII GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
NheI GCTAGC 1 cut(s) 1084
NlaIII CATG 3 cut(s) 703, 1076, 1096
NlaIV GGNNCC 1 cut(s) 989
NmuCI GTSAC 1 cut(s) 567
NspV TTCGAA 1 cut(s) 715
PaeR7I CTCGAG 3 cut(s) 395, 512, 1178
PceI AGGCCT 1 cut(s) 592
PciSI GCTCTTC 1 cut(s) 246
PdmI GAANNNNTTC 1 cut(s) 1020
PfeI GAWTC 1 cut(s) 695
PflFI GACNNNGTC 1 cut(s) 628
PfoI TCCNGGA 1 cut(s) 107
PkrI GCNGC 3 cut(s) 318, 765, 1090
PleI GAGTC 2 cut(s) 53, 619
PpsI GAGTC 2 cut(s) 53, 619
Psp6I CCWGG 2 cut(s) 866, 1035
PspGI CCWGG 2 cut(s) 866, 1035
PspN4I GGNNCC 1 cut(s) 989
PspPI GGNCC 1 cut(s) 218
PspXI VCTCGAGB 1 cut(s) 395
PstI CTGCAG 1 cut(s) 637
PsuI RGATCY 3 cut(s) 751, 785, 1132
PsyI GACNNNGTC 1 cut(s) 628
RsaI GTAC 1 cut(s) 1163
RsaNI GTAC 1 cut(s) 1162
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 4 cut(s) 458, 522, 857, 1109
SatI GCNGC 3 cut(s) 317, 764, 1089
Sau3AI GATC 6 cut(s) 367, 490, 751, 785, 810, 1132
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 2 cut(s) 53, 619
ScrFI CCNGG 3 cut(s) 109, 868, 1037
SduI GDGCHC 1 cut(s) 541
SfaNI GCATC 5 cut(s) 274, 292, 637, 952, 1008
SfcI CTRYAG 1 cut(s) 633
Sfr274I CTCGAG 3 cut(s) 395, 512, 1178
SfuI TTCGAA 1 cut(s) 715
SinI GGWCC 1 cut(s) 218
SlaI CTCGAG 3 cut(s) 395, 512, 1178
SmlI CTYRAG 5 cut(s) 131, 395, 512, 1136, 1178
SmoI CTYRAG 5 cut(s) 131, 395, 512, 1136, 1178
Sse9I AATT 8 cut(s) 233, 425, 452, 503, 560, 711, 889, 1020
SseBI AGGCCT 1 cut(s) 592
SsiI CCGC 2 cut(s) 201, 498
SspMI CTAG 5 cut(s) 554, 755, 996, 1085, 1166
StuI AGGCCT 1 cut(s) 592
StyD4I CCNGG 3 cut(s) 107, 866, 1035
StyI CCWWGG 2 cut(s) 553, 1092
TaiI ACGT 1 cut(s) 1057
TaqI TCGA 7 cut(s) 273, 396, 489, 513, 715, 1057, 1179
TaqII GACCGA 1 cut(s) 235
TasI AATT 8 cut(s) 233, 425, 452, 503, 560, 711, 889, 1020
TfiI GAWTC 1 cut(s) 695
Tru1I TTAA 4 cut(s) 458, 522, 857, 1109
Tru9I TTAA 4 cut(s) 458, 522, 857, 1109
TscAI CASTG 1 cut(s) 972
TseFI GTSAC 1 cut(s) 567
TseI GCWGC 3 cut(s) 316, 763, 1088
Tsp45I GTSAC 1 cut(s) 567
TspDTI ATGAA 5 cut(s) 222, 246, 345, 362, 1089
TspGWI ACGGA 1 cut(s) 1117
TspRI CASTG 1 cut(s) 972
Tth111I GACNNNGTC 1 cut(s) 628
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 3 cut(s) 233, 503, 1020
XbaI TCTAGA 1 cut(s) 754
XhoI CTCGAG 3 cut(s) 395, 512, 1178
XmaJI CCTAGG 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 1020
XspI CTAG 5 cut(s) 554, 755, 996, 1085, 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.