MD03G1060300.v1.1

Transcription initiation factor TFIID subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007365
Physical Location & Seq
Reverse (-)
4866461 .. 4870057
3597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1060300.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGGCTGGAATGTACGGTCAAGAGGGCGATGGCGCCCCTCCTCCGTACGGATCTAGCGGCGGCGGTGGCTATGGTGGCTCTGGCGGGTACGGAGGCGGTTCTGGTGGTTATGGAGGTGGTGGTGGAGGCGGAAGCTATGGTGGAAGCGGCGGTGGTGGCTATGGAGGTAAGGGCGGCGATAGTTATGGAGGCGGTGGCGGACGTGGCGGTGGAGGTTATGGCGGCGGTGGCGGTCGAGGTGGAGGATATCAAGGAGATCGCGGTGGCGGCGGCAGAGGTGGCGACCGTGGTGGAGGCGGCGGCAGAGGCGGCCGCGGTGGCAGTGGAAGAGATGGCGATTGGATGTGCCCTAATCCAGGCTGTGGGAATTTGAACTTTGCAAGAAGAGTTGAGTGTAACAAGTGTGGCACTCCCTCTCCTTCGGGTGCCGCCGGTGGTGATCGTGGCAGTGGTGGTGGCGGCAATTATAACAGAGGAGGAACTGGTGGTGGTAACCGTGGTGGTAGGGGTGGTAACTATGATGGGGGTCGAAGTGGTAACTATGAAGGAGGTAAGGGTAGCAGTTATGACGGAGGCAGAGGCGGTAGTTATGAAAGTAGAGGCGGAGGTGGTAGTAGGGGTGGTTCTTATGGTGGTAGCCAAGGAAGAGATGATGGGGGGTACGGTCAGGTTCCTCCAAATGCACCACCGTCCTATGGCACAGCTGGAGGCAATTACCCATCTTACAATGCTAGTTATGGAACGGATGCAGTTCCGCCTCCTACAAGTTATACTGGTGGACCTGCATCATATCCCCCATCTTATGGTGGTCCTGCAGGCGGTTATGGTGGTGATGGTCCAGGTGATGCAAGGGGTGGTGCCCGAGGGGGCCCTCCTGCTAAATATGATGGTGGATACGGTGCTGGTGGTCGTGGTGGGTATGGAAGTGCTGCTACGGAAGCGCCATCTAAGGTGAAGCAGTGCGACCAGAATTGTGATGATACTTGTGACAATGCTAGAATCTACATATCAAACTTGCCACCAGATGTTACTGTTGACGAACTTCAGCAGCTCTTTGGAGGCATTGGACAAGTGGGAAGAATCAAACAGAAACGTGGCTATAAGGATCAATGGCCATATAATATCAAAATATACACAGATGAGTCTGGAAAAAACAAAGGCGACGCATGTTTAGCCTATGAAGATCCCTCTGCTGCACATTCAGCTGGTGGATTTTACAATGATTATGAGTTAAGGGGCTATAAGATCAGTGTTGCGATGGCGGAGAGGTCAGCTCCAAGGCCTTCGTTTGAACAAGGTGGTGGTGGTGGTGGTGGTAGAGGTGGATATGGGGGCGGTGACAGGCGCAACAACTATAGAGATGGTGGAGCGGATAGACATCAGCATGGTGGAAACCGTTCGCGTCCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
RRM_1 zf-RanBP

Protein Analysis

471

Amino Acids

45.64

Weight (kDa)

8.87

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_RanBP PF00641 110 - 141 8.6e-06 Zn-finger in Ran binding protein and others
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 468
Acc36I ACCTGC 1 cut(s) 790
AccB1I GGYRCC 3 cut(s) 32, 425, 857
AccB7I CCANNNNNTGG 2 cut(s) 362, 803
AccBSI CCGCTC 1 cut(s) 1370
AccII CGCG 3 cut(s) 261, 315, 1402
AclWI GGATC 3 cut(s) 58, 1113, 1178
AcoI YGGCCR 2 cut(s) 310, 1112
AcsI RAATTY 1 cut(s) 367
AcuI CTGAAG 1 cut(s) 1028
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 4 cut(s) 14, 47, 89, 662
AfiI CCNNNNNNNGG 6 cut(s) 47, 356, 362, 695, 803, 818
AgsI TTSAA 2 cut(s) 373, 1292
AjiI CACGTC 1 cut(s) 203
AjnI CCWGG 2 cut(s) 355, 838
AluBI AGCT 5 cut(s) 135, 704, 1051, 1205, 1274
AluI AGCT 5 cut(s) 135, 704, 1051, 1205, 1274
AlwI GGATC 3 cut(s) 58, 1113, 1178
Ama87I CYCGRG 1 cut(s) 861
AoxI GGCC 4 cut(s) 310, 868, 1112, 1280
ApaI GGGCCC 1 cut(s) 872
ApeKI GCWGC 3 cut(s) 929, 1048, 1193
ApoI RAATTY 1 cut(s) 367
Asp700I GAANNNNTTC 1 cut(s) 1396
AspLEI GCGC 3 cut(s) 35, 943, 1347
AspS9I GGNCC 5 cut(s) 779, 809, 836, 868, 869
AsuHPI GGTGA 5 cut(s) 449, 842, 854, 964, 1349
AvaI CYCGRG 1 cut(s) 861
AvaII GGWCC 3 cut(s) 779, 809, 836
BaeGI GKGCMC 3 cut(s) 350, 862, 872
BalI TGGCCA 1 cut(s) 1114
BanI GGYRCC 3 cut(s) 32, 425, 857
BanII GRGCYC 1 cut(s) 872
BarI GAAGNNNNNNTAC 2 cut(s) 916, 948
BbvI GCAGC 3 cut(s) 916, 1060, 1180
BciT130I CCWGG 2 cut(s) 357, 840
BciVI GTATCC 1 cut(s) 887
BfaI CTAG 3 cut(s) 54, 732, 996
BfmI CTRYAG 2 cut(s) 813, 1354
BfoI RGCGCY 2 cut(s) 36, 944
BfuAI ACCTGC 1 cut(s) 790
BfuI GTATCC 1 cut(s) 887
BglI GCCNNNNNGGC 1 cut(s) 318
Bme1390I CCNGG 2 cut(s) 357, 840
Bme18I GGWCC 3 cut(s) 779, 809, 836
BmeT110I CYCGRG 1 cut(s) 861
BmgBI CACGTC 1 cut(s) 203
BmgT120I GGNCC 5 cut(s) 779, 809, 836, 868, 869
BmiI GGNNCC 6 cut(s) 34, 427, 672, 859, 869, 870
BmrFI CCNGG 2 cut(s) 357, 840
BmsI GCATC 3 cut(s) 736, 794, 835
BplI GAGNNNNNCTC 2 cut(s) 1258, 1290
BpmI CTGGAG 1 cut(s) 726
BsaBI GATNNNNATC 1 cut(s) 1377
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 6 cut(s) 286, 313, 496, 640, 862, 1277
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bsc4I CCNNNNNNNGG 6 cut(s) 47, 356, 362, 695, 803, 818
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 487, 778
Bse8I GATNNNNATC 1 cut(s) 1377
BseBI CCWGG 2 cut(s) 357, 840
BseDI CCNNGG 6 cut(s) 286, 313, 496, 640, 862, 1277
BseGI GGATG 2 cut(s) 348, 751
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 6 cut(s) 47, 356, 362, 695, 803, 818
BseNI ACTGG 2 cut(s) 487, 778
BseRI GAGGAG 2 cut(s) 30, 489
BseSI GKGCMC 3 cut(s) 350, 862, 872
BseX3I CGGCCG 1 cut(s) 310
BseXI GCAGC 3 cut(s) 916, 1060, 1180
BsgI GTGCAG 1 cut(s) 1179
Bsh1236I CGCG 3 cut(s) 261, 315, 1402
Bsh1285I CGRYCG 3 cut(s) 235, 286, 313
BshFI GGCC 4 cut(s) 312, 870, 1114, 1282
BshNI GGYRCC 3 cut(s) 32, 425, 857
BsiEI CGRYCG 3 cut(s) 235, 286, 313
BsiHKCI CYCGRG 1 cut(s) 861
BsiSI CCGG 1 cut(s) 432
BsiWI CGTACG 1 cut(s) 45
BslI CCNNNNNNNGG 6 cut(s) 47, 356, 362, 695, 803, 818
BsnI GGCC 4 cut(s) 312, 870, 1114, 1282
BsoBI CYCGRG 1 cut(s) 861
Bsp120I GGGCCC 1 cut(s) 868
Bsp1286I GDGCHC 3 cut(s) 350, 862, 872
Bsp143I GATC 6 cut(s) 50, 256, 439, 1105, 1183, 1245
BspANI GGCC 4 cut(s) 312, 870, 1114, 1282
BspFNI CGCG 3 cut(s) 261, 315, 1402
BspLI GGNNCC 6 cut(s) 34, 427, 672, 859, 869, 870
BspMAI CTGCAG 1 cut(s) 817
BspMI ACCTGC 1 cut(s) 790
BspPI GGATC 3 cut(s) 58, 1113, 1178
BspT107I GGYRCC 3 cut(s) 32, 425, 857
BsrBI CCGCTC 1 cut(s) 1370
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 2 cut(s) 487, 778
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 6 cut(s) 286, 313, 496, 640, 862, 1277
BssMI GATC 6 cut(s) 50, 256, 439, 1105, 1183, 1245
BssNI GRCGYC 1 cut(s) 33
BssT1I CCWWGG 2 cut(s) 640, 1277
Bst2UI CCWGG 2 cut(s) 357, 840
Bst4CI ACNGT 8 cut(s) 17, 287, 497, 665, 690, 899, 1033, 1397
Bst6I CTCTTC 3 cut(s) 322, 379, 640
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 1 cut(s) 817
BstDEI CTNAG 1 cut(s) 948
BstDSI CCRYGG 3 cut(s) 286, 313, 496
BstEII GGTNACC 1 cut(s) 491
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 348, 751
BstFNI CGCG 3 cut(s) 261, 315, 1402
BstH2I RGCGCY 2 cut(s) 36, 944
BstHHI GCGC 3 cut(s) 35, 943, 1347
BstKTI GATC 6 cut(s) 53, 259, 442, 1108, 1186, 1248
BstMBI GATC 6 cut(s) 50, 256, 439, 1105, 1183, 1245
BstMCI CGRYCG 3 cut(s) 235, 286, 313
BstNI CCWGG 2 cut(s) 357, 840
BstNSI RCATGY 1 cut(s) 1170
BstPI GGTNACC 1 cut(s) 491
BstSCI CCNGG 2 cut(s) 355, 838
BstSFI CTRYAG 2 cut(s) 813, 1354
BstSLI GKGCMC 3 cut(s) 350, 862, 872
BstUI CGCG 3 cut(s) 261, 315, 1402
BstV1I GCAGC 3 cut(s) 916, 1060, 1180
BstX2I RGATCY 2 cut(s) 50, 1183
BstYI RGATCY 2 cut(s) 50, 1183
BstZI CGGCCG 1 cut(s) 310
BsuI GTATCC 1 cut(s) 887
BsuRI GGCC 4 cut(s) 312, 870, 1114, 1282
BtgI CCRYGG 3 cut(s) 286, 313, 496
BtgZI GCGATG 2 cut(s) 42, 1271
BtrI CACGTC 1 cut(s) 203
BtsCI GGATG 2 cut(s) 348, 751
BtsI GCAGTG 3 cut(s) 328, 454, 965
BtsIMutI CAGTG 4 cut(s) 328, 454, 965, 1255
BveI ACCTGC 1 cut(s) 790
Cac8I GCNNGC 1 cut(s) 817
CciNI GCGGCCGC 1 cut(s) 310
CfoI GCGC 3 cut(s) 35, 943, 1347
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 5 cut(s) 779, 809, 836, 868, 869
Cfr42I CCGCGG 1 cut(s) 316
CseI GACGC 2 cut(s) 1172, 1391
Csp6I GTAC 4 cut(s) 13, 46, 88, 661
CviAII CATG 2 cut(s) 1167, 1385
CviQI GTAC 4 cut(s) 13, 46, 88, 661
DdeI CTNAG 1 cut(s) 948
DinI GGCGCC 1 cut(s) 34
DpnI GATC 6 cut(s) 52, 258, 441, 1107, 1185, 1247
DpnII GATC 6 cut(s) 50, 256, 439, 1105, 1183, 1245
EaeI YGGCCR 2 cut(s) 310, 1112
EagI CGGCCG 1 cut(s) 310
Eam1104I CTCTTC 3 cut(s) 322, 379, 640
EarI CTCTTC 3 cut(s) 322, 379, 640
EciI GGCGGA 5 cut(s) 144, 213, 618, 744, 1277
EclXI CGGCCG 1 cut(s) 310
Eco130I CCWWGG 2 cut(s) 640, 1277
Eco147I AGGCCT 1 cut(s) 1282
Eco24I GRGCYC 1 cut(s) 872
Eco32I GATATC 1 cut(s) 248
Eco47I GGWCC 3 cut(s) 779, 809, 836
Eco52I CGGCCG 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 1028
Eco88I CYCGRG 1 cut(s) 861
Eco91I GGTNACC 1 cut(s) 491
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoO109I RGGNCCY 2 cut(s) 868, 869
EcoO65I GGTNACC 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 355, 838
EcoRV GATATC 1 cut(s) 248
EcoT14I CCWWGG 2 cut(s) 640, 1277
EcoT38I GRGCYC 1 cut(s) 872
EgeI GGCGCC 1 cut(s) 34
EheI GGCGCC 1 cut(s) 34
ErhI CCWWGG 2 cut(s) 640, 1277
FaeI CATG 2 cut(s) 1170, 1388
FatI CATG 2 cut(s) 1166, 1384
FauI CCCGC 1 cut(s) 77
FokI GGATG 2 cut(s) 355, 758
FriOI GRGCYC 1 cut(s) 872
FspBI CTAG 3 cut(s) 54, 732, 996
GlaI GCGC 3 cut(s) 34, 942, 1346
GsuI CTGGAG 1 cut(s) 726
HaeII RGCGCY 2 cut(s) 36, 944
HaeIII GGCC 4 cut(s) 312, 870, 1114, 1282
HapII CCGG 1 cut(s) 432
HgaI GACGC 2 cut(s) 1172, 1391
HhaI GCGC 3 cut(s) 35, 943, 1347
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 2 cut(s) 1170, 1388
Hin6I GCGC 3 cut(s) 33, 941, 1345
HinP1I GCGC 3 cut(s) 33, 941, 1345
HincII GTYRAC 1 cut(s) 1036
HindII GTYRAC 1 cut(s) 1036
HinfI GANTC 3 cut(s) 999, 1080, 1142
HpaII CCGG 1 cut(s) 432
HphI GGTGA 5 cut(s) 449, 842, 854, 964, 1349
Hpy166II GTNNAC 2 cut(s) 779, 1036
Hpy188III TCNNGA 2 cut(s) 20, 1146
Hpy8I GTNNAC 2 cut(s) 779, 1036
Hpy99I CGWCG 1 cut(s) 1166
HpyAV CCTTC 3 cut(s) 429, 539, 1293
HpyCH4III ACNGT 8 cut(s) 17, 287, 497, 665, 690, 899, 1033, 1397
HpyCH4IV ACGT 2 cut(s) 202, 1093
HpyCH4V TGCA 7 cut(s) 380, 683, 749, 785, 815, 848, 1196
HpyF3I CTNAG 1 cut(s) 948
HpySE526I ACGT 2 cut(s) 202, 1093
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 2 cut(s) 1170, 1388
HspAI GCGC 3 cut(s) 33, 941, 1345
KasI GGCGCC 1 cut(s) 32
KspI CCGCGG 1 cut(s) 316
Kzo9I GATC 6 cut(s) 50, 256, 439, 1105, 1183, 1245
LmnI GCTCC 2 cut(s) 1279, 1367
Lsp1109I GCAGC 3 cut(s) 916, 1060, 1180
LweI GCATC 3 cut(s) 736, 794, 835
MaeI CTAG 3 cut(s) 54, 732, 996
MaeII ACGT 2 cut(s) 202, 1093
MaeIII GTNAC 7 cut(s) 395, 491, 512, 536, 986, 1027, 1337
MalI GATC 6 cut(s) 52, 258, 441, 1107, 1185, 1247
MbiI CCGCTC 1 cut(s) 1370
MboI GATC 6 cut(s) 50, 256, 439, 1105, 1183, 1245
MboII GAAGA 5 cut(s) 339, 396, 657, 1089, 1193
MflI RGATCY 2 cut(s) 50, 1183
MhlI GDGCHC 3 cut(s) 350, 862, 872
MlsI TGGCCA 1 cut(s) 1114
MluCI AATT 4 cut(s) 367, 463, 712, 970
MluNI TGGCCA 1 cut(s) 1114
Mly113I GGCGCC 1 cut(s) 33
MlyI GAGTC 1 cut(s) 1151
Mox20I TGGCCA 1 cut(s) 1114
MroXI GAANNNNTTC 1 cut(s) 1396
MscI TGGCCA 1 cut(s) 1114
MseI TTAA 1 cut(s) 1232
MslI CAYNNNNRTG 1 cut(s) 1383
Msp20I TGGCCA 1 cut(s) 1114
MspA1I CMGCKG 3 cut(s) 315, 704, 1205
MspI CCGG 1 cut(s) 432
MspR9I CCNGG 2 cut(s) 357, 840
MvaI CCWGG 2 cut(s) 357, 840
MvnI CGCG 3 cut(s) 261, 315, 1402
NarI GGCGCC 1 cut(s) 33
NdeII GATC 6 cut(s) 50, 256, 439, 1105, 1183, 1245
NlaIII CATG 2 cut(s) 1170, 1388
NlaIV GGNNCC 6 cut(s) 34, 427, 672, 859, 869, 870
NmuCI GTSAC 2 cut(s) 986, 1337
NotI GCGGCCGC 1 cut(s) 310
NspI RCATGY 1 cut(s) 1170
PceI AGGCCT 1 cut(s) 1282
PdmI GAANNNNTTC 1 cut(s) 1396
PfeI GAWTC 2 cut(s) 999, 1080
Pfl23II CGTACG 1 cut(s) 45
PflMI CCANNNNNTGG 2 cut(s) 362, 803
PleI GAGTC 1 cut(s) 1150
PluTI GGCGCC 1 cut(s) 36
PpsI GAGTC 1 cut(s) 1150
PsiI TTATAA 1 cut(s) 468
Psp6I CCWGG 2 cut(s) 355, 838
PspEI GGTNACC 1 cut(s) 491
PspGI CCWGG 2 cut(s) 355, 838
PspLI CGTACG 1 cut(s) 45
PspN4I GGNNCC 6 cut(s) 34, 427, 672, 859, 869, 870
PspOMI GGGCCC 1 cut(s) 868
PspPI GGNCC 5 cut(s) 779, 809, 836, 868, 869
PsrI GAACNNNNNNTAC 2 cut(s) 607, 639
PstI CTGCAG 1 cut(s) 817
PsuI RGATCY 2 cut(s) 50, 1183
PvuII CAGCTG 2 cut(s) 704, 1205
RsaI GTAC 4 cut(s) 14, 47, 89, 662
RsaNI GTAC 4 cut(s) 13, 46, 88, 661
RseI CAYNNNNRTG 1 cut(s) 1383
SacII CCGCGG 1 cut(s) 316
SaqAI TTAA 1 cut(s) 1232
Sau3AI GATC 6 cut(s) 50, 256, 439, 1105, 1183, 1245
Sau96I GGNCC 5 cut(s) 779, 809, 836, 868, 869
SbfI CCTGCAGG 1 cut(s) 817
SchI GAGTC 1 cut(s) 1151
ScrFI CCNGG 2 cut(s) 357, 840
SdaI CCTGCAGG 1 cut(s) 817
SduI GDGCHC 3 cut(s) 350, 862, 872
SfaNI GCATC 3 cut(s) 736, 794, 835
SfcI CTRYAG 2 cut(s) 813, 1354
SfoI GGCGCC 1 cut(s) 34
Sfr303I CCGCGG 1 cut(s) 316
SgrAI CRCCGGYG 1 cut(s) 431
SgrBI CCGCGG 1 cut(s) 316
SinI GGWCC 3 cut(s) 779, 809, 836
SmiMI CAYNNNNRTG 1 cut(s) 1383
Sse8387I CCTGCAGG 1 cut(s) 817
Sse9I AATT 4 cut(s) 367, 463, 712, 970
SseBI AGGCCT 1 cut(s) 1282
SspDI GGCGCC 1 cut(s) 32
SspMI CTAG 3 cut(s) 54, 732, 996
StuI AGGCCT 1 cut(s) 1282
StyD4I CCNGG 2 cut(s) 355, 838
StyI CCWWGG 2 cut(s) 640, 1277
TaaI ACNGT 8 cut(s) 17, 287, 497, 665, 690, 899, 1033, 1397
TaiI ACGT 2 cut(s) 205, 1096
TaqI TCGA 2 cut(s) 235, 529
TasI AATT 4 cut(s) 367, 463, 712, 970
TfiI GAWTC 2 cut(s) 999, 1080
Tru1I TTAA 1 cut(s) 1232
Tru9I TTAA 1 cut(s) 1232
TscAI CASTG 4 cut(s) 328, 454, 965, 1255
TseFI GTSAC 2 cut(s) 986, 1337
TseI GCWGC 3 cut(s) 929, 1048, 1193
Tsp45I GTSAC 2 cut(s) 986, 1337
TspDTI ATGAA 3 cut(s) 558, 606, 1194
TspGWI ACGGA 6 cut(s) 33, 63, 105, 585, 758, 950
TspRI CASTG 4 cut(s) 328, 454, 965, 1255
Van91I CCANNNNNTGG 2 cut(s) 362, 803
VpaK11BI GGWCC 3 cut(s) 779, 809, 836
XagI CCTNNNNNAGG 1 cut(s) 354
XapI RAATTY 1 cut(s) 367
XceI RCATGY 1 cut(s) 1170
XmnI GAANNNNTTC 1 cut(s) 1396
XspI CTAG 3 cut(s) 54, 732, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.