MD11G1063400.v1.1

Transcription initiation factor TFIID subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007365
Physical Location & Seq
Reverse (-)
5490996 .. 5494788
3793 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1063400.v1.1.491

Sequence Viewer

Length: 1398 bp
ATGGCTGGAATCTACGGTCAAGGGGGCGATGGTGCCCCTCCGACATACGGATCTAGCGGCGGCGGCGGCGGCTATGGTGGTTCTGGCGGATACGGAGGCGGTTCTGGTGGTTATGGAGGTGGTGGTGGAGGCGGGAGCTATGGTGGAAGCGGTGGCGGTGGATATGGAGGTAAGGGCGGCGATGGAGGTGGTTACGGTGGTGGACGGGGCGGTGGCGGTCGAGGTGGAGGATATCAAGGCGACCGTGGTGGTGGAGGCAGAGGTGGCGACCGTGGTGGTGGCGGCAGAGGCGGCCGCGGTGGCAGTGGAAGAGATGGTGATTGGCTGTGTCCTAATCCAGGCTGTGGGAATTCGAACTTTGCAAGAAGAGTTGAGTGTAATAAGTGTGGCACGCCCTCTCCTTCGGGTGCCGGTGGTGATCGTGGCAGTGGTGGTGGTTATAACAGAGGAGGAAGTGGTGGGGGCTACGGTGGCGGTAACCGTGGTGGTAGGGGTGGTAATTATGATGGGGGTCGAAGTGGTAACTATGAAGGAGGTAAAAGTAGCAGTTATGATGGAGGCAGAGGCGGTAGTTATGACAGTAGAGGCGGTGGTGGTAGTAGAGGTGGTTCTTATGGTGGTAGCCAAGGAAGAGATGATGGTGGGTATGGTCAGGTTCCTCCAAGTGCTCCACCGTCCTATGGTGCAGCTGGAGGCAATTACGCACCATCTTACAATGCTGGTTATGGAACAGATGCAGTTCCTCCTCCTACAAGCTATACTGGTGGACCTGGATCGTATCCCCCATCTTACGGTGGTCCTGCAGGCGGTTATGGTGGTGATGGTCTAGGTGATGCAAGGGGTGGTGGGCGTGGGGGCCCTCCAGCTAAATATGATGGTGGATACAGTGCTGGTGGTCGTGGTGGATATGGCAGTGGTGCTACAGAAGCCGCAGCTAAGGTGAAGCAGTGCGACCAGAATTGTGATGATACTTGTGACAATGCTAGAATCTACATATCAAACTTACCACCAGATGTTACCGTTGACGAACTTCAGCAGCTCTTTGGAGGCATTGGACAAGTGGGAAGAATCAAGCAGAAACGTGGCTATAAGGATCAATGGCCATATAATATCAAAATATACACAGACGAGTCTGGAAAAAACAAAGGCGACGCATGTTTAGCCTATGAAGATCCCTCTGCTGCACATTCAGCTGGTGGATTTTACAATGATTATGACTTAAGGGGCTATAAGATCAGTGTTACAATGGCGGAGAGATCAGCTCCAAGGCCTTCGTTTGAACAAGGCAGTGGTGGTGGTGGTAGAGGTGGATATGGTGGCGGTGACAGGCGCAACAGTTATAGAGACGGTGGAGCTGATAGACATCAACACGGCGGAAACCGTTCGCGTCCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
RRM_1 zf-RanBP

Protein Analysis

466

Amino Acids

45.03

Weight (kDa)

8.95

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_RanBP PF00641 104 - 135 3.5e-07 Zn-finger in Ran binding protein and others
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 441
AccB1I GGYRCC 2 cut(s) 32, 407
AccB7I CCANNNNNTGG 1 cut(s) 344
AccII CGCG 2 cut(s) 297, 1387
AclWI GGATC 4 cut(s) 58, 781, 1101, 1166
AcoI YGGCCR 2 cut(s) 292, 1100
AcsI RAATTY 1 cut(s) 349
AcuI CTGAAG 1 cut(s) 1016
AfiI CCNNNNNNNGG 6 cut(s) 47, 338, 344, 680, 791, 806
AflII CTTAAG 1 cut(s) 1219
AgsI TTSAA 1 cut(s) 1280
AjnI CCWGG 2 cut(s) 337, 769
AluBI AGCT 9 cut(s) 138, 689, 756, 866, 935, 1039, 1193, 1262, 1355
AluI AGCT 9 cut(s) 138, 689, 756, 866, 935, 1039, 1193, 1262, 1355
Alw21I GWGCWC 1 cut(s) 670
Alw26I GTCTC 1 cut(s) 1338
AlwI GGATC 4 cut(s) 58, 781, 1101, 1166
AoxI GGCC 4 cut(s) 292, 856, 1100, 1268
ApaI GGGCCC 1 cut(s) 860
ApeKI GCWGC 4 cut(s) 686, 932, 1036, 1181
ApoI RAATTY 1 cut(s) 349
Asp700I GAANNNNTTC 1 cut(s) 1381
AspLEI GCGC 1 cut(s) 1332
AspS9I GGNCC 4 cut(s) 767, 797, 856, 857
AsuHPI GGTGA 6 cut(s) 329, 428, 830, 842, 952, 1334
AsuII TTCGAA 1 cut(s) 353
AvaII GGWCC 2 cut(s) 767, 797
BaeGI GKGCMC 2 cut(s) 37, 860
BalI TGGCCA 1 cut(s) 1102
BanI GGYRCC 2 cut(s) 32, 407
BanII GRGCYC 1 cut(s) 860
Bbv12I GWGCWC 1 cut(s) 670
BbvI GCAGC 4 cut(s) 698, 944, 1048, 1168
BceAI ACGGC 1 cut(s) 1387
BciT130I CCWGG 2 cut(s) 339, 771
BciVI GTATCC 3 cut(s) 83, 789, 875
BcoDI GTCTC 1 cut(s) 1338
BfaI CTAG 3 cut(s) 54, 827, 984
BfmI CTRYAG 2 cut(s) 801, 921
BfrI CTTAAG 1 cut(s) 1219
BfuI GTATCC 3 cut(s) 83, 789, 875
BglI GCCNNNNNGGC 1 cut(s) 300
Bme1390I CCNGG 2 cut(s) 339, 771
Bme18I GGWCC 2 cut(s) 767, 797
BmgT120I GGNCC 4 cut(s) 767, 797, 856, 857
BmiI GGNNCC 5 cut(s) 34, 409, 657, 857, 858
BmrFI CCNGG 2 cut(s) 339, 771
BmsI GCATC 2 cut(s) 724, 823
BplI GAGNNNNNCTC 2 cut(s) 1246, 1278
BpmI CTGGAG 2 cut(s) 711, 846
Bpu10I CCTNAGC 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 1362
BsaJI CCNNGG 6 cut(s) 244, 271, 295, 481, 625, 1265
BsaXI ACNNNNNCTCC 4 cut(s) 127, 157, 382, 412
Bsc4I CCNNNNNNNGG 6 cut(s) 47, 338, 344, 680, 791, 806
Bse118I RCCGGY 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 766
Bse8I GATNNNNATC 1 cut(s) 1362
BseBI CCWGG 2 cut(s) 339, 771
BseDI CCNNGG 6 cut(s) 244, 271, 295, 481, 625, 1265
BseJI GATNNNNATC 1 cut(s) 1362
BseLI CCNNNNNNNGG 6 cut(s) 47, 338, 344, 680, 791, 806
BseNI ACTGG 1 cut(s) 766
BseRI GAGGAG 2 cut(s) 462, 735
BseSI GKGCMC 2 cut(s) 37, 860
BseX3I CGGCCG 1 cut(s) 292
BseXI GCAGC 4 cut(s) 698, 944, 1048, 1168
BsgI GTGCAG 2 cut(s) 705, 1167
Bsh1236I CGCG 2 cut(s) 297, 1387
Bsh1285I CGRYCG 4 cut(s) 220, 244, 271, 295
BshFI GGCC 4 cut(s) 294, 858, 1102, 1270
BshNI GGYRCC 2 cut(s) 32, 407
BsiEI CGRYCG 4 cut(s) 220, 244, 271, 295
BsiHKAI GWGCWC 1 cut(s) 670
BsiSI CCGG 1 cut(s) 411
BslI CCNNNNNNNGG 6 cut(s) 47, 338, 344, 680, 791, 806
BsmAI GTCTC 1 cut(s) 1338
BsmBI CGTCTC 1 cut(s) 1338
BsnI GGCC 4 cut(s) 294, 858, 1102, 1270
Bsp119I TTCGAA 1 cut(s) 353
Bsp120I GGGCCC 1 cut(s) 856
Bsp1286I GDGCHC 3 cut(s) 37, 670, 860
Bsp143I GATC 7 cut(s) 50, 418, 773, 1093, 1171, 1233, 1256
BspANI GGCC 4 cut(s) 294, 858, 1102, 1270
BspFNI CGCG 2 cut(s) 297, 1387
BspLI GGNNCC 5 cut(s) 34, 409, 657, 857, 858
BspMAI CTGCAG 1 cut(s) 805
BspPI GGATC 4 cut(s) 58, 781, 1101, 1166
BspT104I TTCGAA 1 cut(s) 353
BspT107I GGYRCC 2 cut(s) 32, 407
BspTI CTTAAG 1 cut(s) 1219
BsrFI RCCGGY 1 cut(s) 410
BsrI ACTGG 1 cut(s) 766
BssAI RCCGGY 1 cut(s) 410
BssECI CCNNGG 6 cut(s) 244, 271, 295, 481, 625, 1265
BssMI GATC 7 cut(s) 50, 418, 773, 1093, 1171, 1233, 1256
BssT1I CCWWGG 2 cut(s) 625, 1265
Bst2UI CCWGG 2 cut(s) 339, 771
Bst6I CTCTTC 3 cut(s) 304, 361, 625
BstAFI CTTAAG 1 cut(s) 1219
BstBI TTCGAA 1 cut(s) 353
BstC8I GCNNGC 2 cut(s) 392, 805
BstDEI CTNAG 1 cut(s) 936
BstDSI CCRYGG 4 cut(s) 244, 271, 295, 481
BstEII GGTNACC 1 cut(s) 476
BstENI CCTNNNNNAGG 1 cut(s) 336
BstFNI CGCG 2 cut(s) 297, 1387
BstHHI GCGC 1 cut(s) 1332
BstKTI GATC 7 cut(s) 53, 421, 776, 1096, 1174, 1236, 1259
BstMAI GTCTC 1 cut(s) 1338
BstMBI GATC 7 cut(s) 50, 418, 773, 1093, 1171, 1233, 1256
BstMCI CGRYCG 4 cut(s) 220, 244, 271, 295
BstNI CCWGG 2 cut(s) 339, 771
BstNSI RCATGY 1 cut(s) 1158
BstPI GGTNACC 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 337, 769
BstSFI CTRYAG 2 cut(s) 801, 921
BstSLI GKGCMC 2 cut(s) 37, 860
BstUI CGCG 2 cut(s) 297, 1387
BstV1I GCAGC 4 cut(s) 698, 944, 1048, 1168
BstX2I RGATCY 2 cut(s) 50, 1171
BstYI RGATCY 2 cut(s) 50, 1171
BstZI CGGCCG 1 cut(s) 292
BsuI GTATCC 3 cut(s) 83, 789, 875
BsuRI GGCC 4 cut(s) 294, 858, 1102, 1270
BtgI CCRYGG 4 cut(s) 244, 271, 295, 481
BtgZI GCGATG 2 cut(s) 42, 195
BtsI GCAGTG 5 cut(s) 310, 433, 919, 953, 1294
BtsIMutI CAGTG 7 cut(s) 310, 433, 892, 919, 953, 1243, 1294
Cac8I GCNNGC 2 cut(s) 392, 805
CciNI GCGGCCGC 1 cut(s) 292
CfoI GCGC 1 cut(s) 1332
Cfr10I RCCGGY 1 cut(s) 410
Cfr13I GGNCC 4 cut(s) 767, 797, 856, 857
Cfr42I CCGCGG 1 cut(s) 298
CseI GACGC 2 cut(s) 1160, 1376
CviAII CATG 1 cut(s) 1155
DdeI CTNAG 1 cut(s) 936
DpnI GATC 7 cut(s) 52, 420, 775, 1095, 1173, 1235, 1258
DpnII GATC 7 cut(s) 50, 418, 773, 1093, 1171, 1233, 1256
EaeI YGGCCR 2 cut(s) 292, 1100
EagI CGGCCG 1 cut(s) 292
Eam1104I CTCTTC 3 cut(s) 304, 361, 625
EarI CTCTTC 3 cut(s) 304, 361, 625
EciI GGCGGA 3 cut(s) 102, 1265, 1389
EclXI CGGCCG 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 625, 1265
Eco147I AGGCCT 1 cut(s) 1270
Eco24I GRGCYC 1 cut(s) 860
Eco32I GATATC 1 cut(s) 233
Eco47I GGWCC 2 cut(s) 767, 797
Eco52I CGGCCG 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 1016
Eco91I GGTNACC 1 cut(s) 476
EcoNI CCTNNNNNAGG 1 cut(s) 336
EcoO109I RGGNCCY 2 cut(s) 856, 857
EcoO65I GGTNACC 1 cut(s) 476
EcoRI GAATTC 1 cut(s) 349
EcoRII CCWGG 2 cut(s) 337, 769
EcoRV GATATC 1 cut(s) 233
EcoT14I CCWWGG 2 cut(s) 625, 1265
EcoT38I GRGCYC 1 cut(s) 860
ErhI CCWWGG 2 cut(s) 625, 1265
Esp3I CGTCTC 1 cut(s) 1338
FaeI CATG 1 cut(s) 1158
FatI CATG 1 cut(s) 1154
FauI CCCGC 1 cut(s) 125
FriOI GRGCYC 1 cut(s) 860
FspBI CTAG 3 cut(s) 54, 827, 984
GlaI GCGC 1 cut(s) 1331
GsuI CTGGAG 2 cut(s) 711, 846
HaeIII GGCC 4 cut(s) 294, 858, 1102, 1270
HapII CCGG 1 cut(s) 411
HgaI GACGC 2 cut(s) 1160, 1376
HhaI GCGC 1 cut(s) 1332
Hin1II CATG 1 cut(s) 1158
Hin6I GCGC 1 cut(s) 1330
HinP1I GCGC 1 cut(s) 1330
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HinfI GANTC 4 cut(s) 9, 987, 1068, 1130
HpaII CCGG 1 cut(s) 411
HphI GGTGA 6 cut(s) 329, 428, 830, 842, 952, 1334
Hpy166II GTNNAC 3 cut(s) 203, 767, 1024
Hpy188I TCNGA 1 cut(s) 42
Hpy188III TCNNGA 1 cut(s) 1134
Hpy8I GTNNAC 3 cut(s) 203, 767, 1024
Hpy99I CGWCG 1 cut(s) 1154
HpyAV CCTTC 3 cut(s) 411, 524, 1281
HpyCH4IV ACGT 1 cut(s) 1081
HpyCH4V TGCA 6 cut(s) 362, 686, 737, 803, 836, 1184
HpyF3I CTNAG 1 cut(s) 936
HpySE526I ACGT 1 cut(s) 1081
Hsp92II CATG 1 cut(s) 1158
HspAI GCGC 1 cut(s) 1330
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 7 cut(s) 50, 418, 773, 1093, 1171, 1233, 1256
LmnI GCTCC 4 cut(s) 135, 673, 1267, 1352
Lsp1109I GCAGC 4 cut(s) 698, 944, 1048, 1168
LweI GCATC 2 cut(s) 724, 823
MaeI CTAG 3 cut(s) 54, 827, 984
MaeII ACGT 1 cut(s) 1081
MaeIII GTNAC 7 cut(s) 191, 476, 521, 974, 1015, 1240, 1322
MalI GATC 7 cut(s) 52, 420, 775, 1095, 1173, 1235, 1258
MboI GATC 7 cut(s) 50, 418, 773, 1093, 1171, 1233, 1256
MboII GAAGA 5 cut(s) 321, 378, 642, 1077, 1181
MflI RGATCY 2 cut(s) 50, 1171
MhlI GDGCHC 3 cut(s) 37, 670, 860
MlsI TGGCCA 1 cut(s) 1102
MluCI AATT 4 cut(s) 349, 499, 697, 958
MluNI TGGCCA 1 cut(s) 1102
MlyI GAGTC 1 cut(s) 1139
MmeI TCCRAC 1 cut(s) 65
Mox20I TGGCCA 1 cut(s) 1102
MroXI GAANNNNTTC 1 cut(s) 1381
MscI TGGCCA 1 cut(s) 1102
MseI TTAA 1 cut(s) 1220
Msp20I TGGCCA 1 cut(s) 1102
MspA1I CMGCKG 3 cut(s) 297, 689, 1193
MspCI CTTAAG 1 cut(s) 1219
MspI CCGG 1 cut(s) 411
MspR9I CCNGG 2 cut(s) 339, 771
MvaI CCWGG 2 cut(s) 339, 771
MvnI CGCG 2 cut(s) 297, 1387
NdeII GATC 7 cut(s) 50, 418, 773, 1093, 1171, 1233, 1256
NlaIII CATG 1 cut(s) 1158
NlaIV GGNNCC 5 cut(s) 34, 409, 657, 857, 858
NmuCI GTSAC 2 cut(s) 974, 1322
NotI GCGGCCGC 1 cut(s) 292
NspI RCATGY 1 cut(s) 1158
NspV TTCGAA 1 cut(s) 353
PceI AGGCCT 1 cut(s) 1270
PdmI GAANNNNTTC 1 cut(s) 1381
PfeI GAWTC 3 cut(s) 9, 987, 1068
PflMI CCANNNNNTGG 1 cut(s) 344
PleI GAGTC 1 cut(s) 1138
PpsI GAGTC 1 cut(s) 1138
PsiI TTATAA 1 cut(s) 441
Psp6I CCWGG 2 cut(s) 337, 769
PspEI GGTNACC 1 cut(s) 476
PspGI CCWGG 2 cut(s) 337, 769
PspN4I GGNNCC 5 cut(s) 34, 409, 657, 857, 858
PspOMI GGGCCC 1 cut(s) 856
PspPI GGNCC 4 cut(s) 767, 797, 856, 857
PsrI GAACNNNNNNTAC 2 cut(s) 592, 624
PstI CTGCAG 1 cut(s) 805
PsuI RGATCY 2 cut(s) 50, 1171
PvuII CAGCTG 2 cut(s) 689, 1193
SacII CCGCGG 1 cut(s) 298
SaqAI TTAA 1 cut(s) 1220
Sau3AI GATC 7 cut(s) 50, 418, 773, 1093, 1171, 1233, 1256
Sau96I GGNCC 4 cut(s) 767, 797, 856, 857
SbfI CCTGCAGG 1 cut(s) 805
SchI GAGTC 1 cut(s) 1139
ScrFI CCNGG 2 cut(s) 339, 771
SdaI CCTGCAGG 1 cut(s) 805
SduI GDGCHC 3 cut(s) 37, 670, 860
SfaNI GCATC 2 cut(s) 724, 823
SfcI CTRYAG 2 cut(s) 801, 921
Sfr303I CCGCGG 1 cut(s) 298
SfuI TTCGAA 1 cut(s) 353
SgrBI CCGCGG 1 cut(s) 298
SinI GGWCC 2 cut(s) 767, 797
SmlI CTYRAG 1 cut(s) 1219
SmoI CTYRAG 1 cut(s) 1219
Sse8387I CCTGCAGG 1 cut(s) 805
Sse9I AATT 4 cut(s) 349, 499, 697, 958
SseBI AGGCCT 1 cut(s) 1270
SspMI CTAG 3 cut(s) 54, 827, 984
StuI AGGCCT 1 cut(s) 1270
StyD4I CCNGG 2 cut(s) 337, 769
StyI CCWWGG 2 cut(s) 625, 1265
TaiI ACGT 1 cut(s) 1084
TaqI TCGA 3 cut(s) 220, 353, 514
TasI AATT 4 cut(s) 349, 499, 697, 958
TfiI GAWTC 3 cut(s) 9, 987, 1068
Tru1I TTAA 1 cut(s) 1220
Tru9I TTAA 1 cut(s) 1220
TscAI CASTG 7 cut(s) 310, 433, 892, 919, 953, 1243, 1294
TseFI GTSAC 2 cut(s) 974, 1322
TseI GCWGC 4 cut(s) 686, 932, 1036, 1181
Tsp45I GTSAC 2 cut(s) 974, 1322
TspDTI ATGAA 2 cut(s) 543, 1182
TspGWI ACGGA 2 cut(s) 63, 108
TspRI CASTG 7 cut(s) 310, 433, 892, 919, 953, 1243, 1294
Van91I CCANNNNNTGG 1 cut(s) 344
Vha464I CTTAAG 1 cut(s) 1219
VpaK11BI GGWCC 2 cut(s) 767, 797
XagI CCTNNNNNAGG 1 cut(s) 336
XapI RAATTY 1 cut(s) 349
XceI RCATGY 1 cut(s) 1158
XmnI GAANNNNTTC 1 cut(s) 1381
XspI CTAG 3 cut(s) 54, 827, 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.