Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002215

Physical Location & Seq

Genomic Coordinates Forward (+)

6338435 .. 6340563

Gene Structure 2129 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1078100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1416 bp

ATGGCGGGAAAGGTTTCGAATTTCTCGGATCTGATTCAGCGAGTGGCGGCTTCTTGCTTGCTCCACCCACTCGCCGCCGGGCGCCACGATTCCTGCGATTCCCCCGTGCATCGACGCACCGGCGATGAGCATGACGAGGACGACGGAGATGAGGAAGACGAAGACGATGAAGTGATTGACGGTGGAGGCTTAGGGTTTAAGGCTGGGGAGGATCGGGGGTCGACAAATAAGGAGGTTGTGGAGATGGAGATGTCAATGCGGGAGGTTTTCGACGCCGTTGCGGCGATGAAGAAGGCGTACGTCAGCCTCCAGGAGGCCCACAACCCGTGGGACCCGGCGAAGCTCAGGGCGGCTGACGCTGCGGTGGTCGGTGAGCTGAGAAAAATCGGGGTGCTGAGAGAGAGGTTTCGAAGAAGGCATTTTACCGGCGGGGGAGAGCGAGTGATGGCGGGGAGTACGTTGAGAGAGGTGGTGGCGCCGTACGAAGCGGCAGTGGACGAGCTGAAGAGAGAGGTGAAGTGCAGAGAGGTCGAGATCCAGAACCTCAAGGAGAAGCTAAACGGCGTCGCTAACGGGTCCCACTCTAAGAAGGGCAGGTCCGTGTCCAGAAGAAAAGTCGGTTCCATCGGTCTGACTGAAGTTGCAGCGGCTCCAGCGCCGGAGCTGTTCGAGGCGACGATGAGCCAGGTGAGAGAGGCATCCAAGTCGTTCACGTCGCTGCTCCTCACCCTAATGCGCGCAGCCCACTGGGACATCGCCGCCGCAGTCCGGTCAATTGAAGCCGCCACCGCTACCACCGTCGGGACCAACACGACGTCTTCGACCATCGCTACCCAGAACGGCGACGCCAAGTACGCGCTGCAGTCCTACATTTCGCGGAAGCTCTTCCAGGGATTCGATCACGAGACGTTCTACATGGACGGTAGCCTCTCGTCGCTGCTCAACCCGGACCAGCACCGGCGCGACTGCTTCGCCCGGTACCGCGACTTCAAGGCCATGGACCCCACCGAGCTGCTCGGGATTCTGCCCACGTGTCAGTTCGGGAAGTTCTGCTCCGCCAAGTACCTGGCCGTCGTCCATCCCAAGATGGAGGAGTCGTTGTTTGGGCACTTGGAGCAGCGACGACATGTCGTAGAAGGGGGGCACCCGAGGAGCGAGTTTTACAGGGAATTTTTGGGGCTGGCGAAGGCGGTGTGGCTGCTGCACTTGCTGGCGTTTTCGCTTGACCCGCCGCCGAGTCAGTTCGAGGCGAGTCGGGGAGCGGAGTTTCATCCGGGTTATATGGAGAGTGTTGTGAGGTTTGCGGGCGGGCGAGTTCCGTTGGGTCAGGTTGTCGGGTTTCCGGTTTGTCCCGGGTTTAAGCTTGGGAATGGGTCGGTTATTAAGGCCAGGGTTTATCTGGTAAATAGGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009639 GO:0009959 GO:0050896

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF641

Protein Analysis

472

Amino Acids

51.87

Weight (kDa)

6.62

Isoelectric Point (pI)

43.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF641	PF04859	79 - 193	3.1e-32	Plant protein of unknown function (DUF641)
GIL1_IRKI_C	PF24994	415 - 466	2.9e-10	GIL1/IRKI C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002215)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58960 AT5G58960 AT5G58960
fragaria_vesca	FvH4_3g37820 FvH4_3g37850
malus_domestica	MD03G1078100.v1.1 MD11G1082800.v1.1
prunus_persica	Prupe.6G063100_v2.0.a1
pyrus_communis	pycom03g06240 pycom11g07090
rosa_chinensis	RchiOBHm_Chr5g0068001 RchiOBHm_Chr5g0068051 RchiOBHm_Chr5g0068071
rosa_laevigata	RLG00000035951 RLG00000035952
rosa_multiflora	Rmu_co8216896.1_g000001 Rmu_co8424771.1_g000001 Rmu_sc0000435.1_g000019 Rmu_sc0000435.1_g000025 Rmu_sc0000435.1_g000034
rosa_roxburghii	Rroxscaffold_1G00012550 Rroxscaffold_1G00012560 Rroxscaffold_1G00013280 Rroxscaffold_1G00013290
rosa_rugosa	Rorug05G0387200 Rorug05G0387600 Rorug05G0388100
rosa_samantha	Rh5AG446100 Rh5AG446300 Rh5AG446400 Rh5BG463600 Rh5BG464000 Rh5BG464300 Rh5CG485500 Rh5CG486000 Rh5CG486300 Rh5DG477600 Rh5DG478000 Rh5DG478300
rosa_wichuraiana	Rw5G041670 Rw5G041680 Rw5G041710 Rw5G041720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	818
Acc36I	ACCTGC	1 cut(s)	585
Acc65I	GGTACC	1 cut(s)	978
AccB1I	GGYRCC	4 cut(s)	81, 475, 978, 1143
AccBSI	CCGCTC	1 cut(s)	1262
AccI	GTMKAC	1 cut(s)	221
AccII	CGCG	5 cut(s)	738, 857, 877, 963, 984
AclWI	GGATC	3 cut(s)	36, 219, 529
AcoI	YGGCCR	1 cut(s)	1068
AcsI	RAATTY	2 cut(s)	19, 1169
AcuI	CTGAAG	2 cut(s)	524, 657
AcvI	CACGTG	1 cut(s)	1032
AcyI	GRCGYC	6 cut(s)	82, 273, 476, 564, 815, 846
AfaI	GTAC	6 cut(s)	299, 457, 482, 854, 980, 1064
AfiI	CCNNNNNNNGG	3 cut(s)	313, 768, 801
AflIII	ACRYGT	2 cut(s)	1031, 1126
AgsI	TTSAA	2 cut(s)	779, 991
AhdI	GACNNNNNGTC	1 cut(s)	1127
AjiI	CACGTC	1 cut(s)	714
AjnI	CCWGG	5 cut(s)	309, 684, 888, 1065, 1388
AluBI	AGCT	8 cut(s)	343, 376, 502, 556, 664, 883, 1012, 1363
AluI	AGCT	8 cut(s)	343, 376, 502, 556, 664, 883, 1012, 1363
Alw26I	GTCTC	1 cut(s)	899
AlwI	GGATC	3 cut(s)	36, 219, 529
Ama87I	CYCGRG	3 cut(s)	1016, 1147, 1352
AoxI	GGCC	4 cut(s)	315, 993, 1068, 1386
ApoI	RAATTY	2 cut(s)	19, 1169
Asp700I	GAANNNNTTC	2 cut(s)	13, 884
Asp718I	GGTACC	1 cut(s)	978
AspLEI	GCGC	7 cut(s)	84, 478, 658, 738, 740, 859, 963
AspS9I	GGNCC	7 cut(s)	316, 331, 576, 597, 804, 949, 1000
AsuC2I	CCSGG	7 cut(s)	79, 335, 947, 976, 1275, 1353, 1354
AsuHPI	GGTGA	4 cut(s)	383, 526, 700, 718
AsuII	TTCGAA	2 cut(s)	17, 409
AvaI	CYCGRG	3 cut(s)	1016, 1147, 1352
AvaII	GGWCC	6 cut(s)	331, 576, 597, 804, 949, 1000
BaeGI	GKGCMC	2 cut(s)	1110, 1146
BanI	GGYRCC	4 cut(s)	81, 475, 978, 1143
BarI	GAAGNNNNNNTAC	6 cut(s)	281, 313, 406, 438, 971, 1003
BauI	CACGAG	1 cut(s)	902
BbrPI	CACGTG	1 cut(s)	1032
BbsI	GAAGAC	3 cut(s)	162, 168, 810
BccI	CCATC	6 cut(s)	238, 439, 632, 833, 1081, 1086
BceAI	ACGGC	5 cut(s)	260, 463, 577, 856, 1055
BcgI	CGANNNNNNTGC	6 cut(s)	260, 294, 511, 545, 687, 721
BciT130I	CCWGG	5 cut(s)	311, 686, 890, 1067, 1390
BcnI	CCSGG	7 cut(s)	79, 335, 947, 976, 1275, 1353, 1354
BcoDI	GTCTC	1 cut(s)	899
BfmI	CTRYAG	1 cut(s)	860
BfoI	RGCGCY	3 cut(s)	85, 479, 659
BfuAI	ACCTGC	1 cut(s)	585
BglI	GCCNNNNNGGC	1 cut(s)	281
Bme18I	GGWCC	6 cut(s)	331, 576, 597, 804, 949, 1000
BmeRI	GACNNNNNGTC	1 cut(s)	1127
BmeT110I	CYCGRG	3 cut(s)	1016, 1147, 1352
BmgBI	CACGTC	1 cut(s)	714
BmgT120I	GGNCC	7 cut(s)	316, 331, 576, 597, 804, 949, 1000
BmrI	ACTGGG	1 cut(s)	757
BmsI	GCATC	2 cut(s)	118, 707
BmuI	ACTGGG	1 cut(s)	757
BpiI	GAAGAC	3 cut(s)	162, 168, 810
BpmI	CTGGAG	2 cut(s)	293, 636
Bpu10I	CCTNAGC	2 cut(s)	190, 344
Bpu14I	TTCGAA	2 cut(s)	17, 409
BpuEI	CTTGAG	1 cut(s)	530
BpuMI	CCSGG	7 cut(s)	79, 335, 947, 976, 1275, 1353, 1354
BsaAI	YACGTR	1 cut(s)	1032
BsaHI	GRCGYC	6 cut(s)	82, 273, 476, 564, 815, 846
BsaJI	CCNNGG	6 cut(s)	326, 889, 996, 1148, 1352, 1389
BsaWI	WCCGGW	2 cut(s)	768, 1342
BsaXI	ACNNNNNCTCC	6 cut(s)	426, 456, 1082, 1085, 1112, 1115
Bsc4I	CCNNNNNNNGG	3 cut(s)	313, 768, 801
Bse118I	RCCGGY	3 cut(s)	119, 425, 957
Bse1I	ACTGG	1 cut(s)	752
BseBI	CCWGG	5 cut(s)	311, 686, 890, 1067, 1390
BseDI	CCNNGG	6 cut(s)	326, 889, 996, 1148, 1352, 1389
BseGI	GGATG	3 cut(s)	698, 1078, 1270
BseLI	CCNNNNNNNGG	3 cut(s)	313, 768, 801
BseMII	CTCAG	3 cut(s)	358, 368, 386
BseNI	ACTGG	1 cut(s)	752
BsePI	GCGCGC	1 cut(s)	736
BseRI	GAGGAG	3 cut(s)	713, 1106, 1165
BseSI	GKGCMC	2 cut(s)	1110, 1146
BseYI	CCCAGC	1 cut(s)	203
BsgI	GTGCAG	2 cut(s)	541, 1187
Bsh1236I	CGCG	5 cut(s)	738, 857, 877, 963, 984
BshFI	GGCC	4 cut(s)	317, 995, 1070, 1388
BshNI	GGYRCC	4 cut(s)	81, 475, 978, 1143
BsiHKCI	CYCGRG	3 cut(s)	1016, 1147, 1352
BsiWI	CGTACG	2 cut(s)	297, 480
BslFI	GGGAC	5 cut(s)	344, 562, 764, 817, 1335
BslI	CCNNNNNNNGG	3 cut(s)	313, 768, 801
BsmAI	GTCTC	1 cut(s)	899
BsmBI	CGTCTC	1 cut(s)	899
BsmFI	GGGAC	5 cut(s)	344, 562, 764, 817, 1335
BsnI	GGCC	4 cut(s)	317, 995, 1070, 1388
BsoBI	CYCGRG	3 cut(s)	1016, 1147, 1352
Bsp119I	TTCGAA	2 cut(s)	17, 409
Bsp1286I	GDGCHC	2 cut(s)	1110, 1146
Bsp143I	GATC	4 cut(s)	28, 211, 534, 898
Bsp19I	CCATGG	1 cut(s)	996
BspANI	GGCC	4 cut(s)	317, 995, 1070, 1388
BspCNI	CTCAG	3 cut(s)	357, 369, 387
BspFNI	CGCG	5 cut(s)	738, 857, 877, 963, 984
BspMAI	CTGCAG	1 cut(s)	864
BspMI	ACCTGC	1 cut(s)	585
BspPI	GGATC	3 cut(s)	36, 219, 529
BspQI	GCTCTTC	1 cut(s)	890
BspT104I	TTCGAA	2 cut(s)	17, 409
BspT107I	GGYRCC	4 cut(s)	81, 475, 978, 1143
BsrBI	CCGCTC	1 cut(s)	1262
BsrFI	RCCGGY	3 cut(s)	119, 425, 957
BsrI	ACTGG	1 cut(s)	752
BssAI	RCCGGY	3 cut(s)	119, 425, 957
BssECI	CCNNGG	6 cut(s)	326, 889, 996, 1148, 1352, 1389
BssHII	GCGCGC	1 cut(s)	736
BssMI	GATC	4 cut(s)	28, 211, 534, 898
BssNI	GRCGYC	6 cut(s)	82, 273, 476, 564, 815, 846
BssSI	CACGAG	1 cut(s)	902
BssT1I	CCWWGG	1 cut(s)	996
Bst2BI	CACGAG	1 cut(s)	902
Bst2UI	CCWGG	5 cut(s)	311, 686, 890, 1067, 1390
Bst4CI	ACNGT	3 cut(s)	182, 799, 923
Bst6I	CTCTTC	2 cut(s)	500, 890
BstACI	GRCGYC	6 cut(s)	82, 273, 476, 564, 815, 846
BstBAI	YACGTR	1 cut(s)	1032
BstBI	TTCGAA	2 cut(s)	17, 409
BstC8I	GCNNGC	6 cut(s)	59, 738, 1182, 1212, 1306, 1310
BstDEI	CTNAG	5 cut(s)	190, 344, 377, 395, 585
BstDSI	CCRYGG	2 cut(s)	326, 996
BstENI	CCTNNNNNAGG	1 cut(s)	311
BstF5I	GGATG	3 cut(s)	698, 1078, 1270
BstFNI	CGCG	5 cut(s)	738, 857, 877, 963, 984
BstH2I	RGCGCY	3 cut(s)	85, 479, 659
BstHHI	GCGC	7 cut(s)	84, 478, 658, 738, 740, 859, 963
BstKTI	GATC	4 cut(s)	31, 214, 537, 901
BstMAI	GTCTC	1 cut(s)	899
BstMBI	GATC	4 cut(s)	28, 211, 534, 898
BstMWI	GCNNNNNNNGC	9 cut(s)	281, 356, 359, 653, 788, 854, 1114, 1207, 1228
BstNI	CCWGG	5 cut(s)	311, 686, 890, 1067, 1390
BstNSI	RCATGY	1 cut(s)	1130
BstSFI	CTRYAG	1 cut(s)	860
BstSLI	GKGCMC	2 cut(s)	1110, 1146
BstUI	CGCG	5 cut(s)	738, 857, 877, 963, 984
BstV2I	GAAGAC	3 cut(s)	162, 168, 810
BstX2I	RGATCY	2 cut(s)	28, 534
BstXI	CCANNNNNNTGG	1 cut(s)	1066
BstYI	RGATCY	2 cut(s)	28, 534
BsuRI	GGCC	4 cut(s)	317, 995, 1070, 1388
BtgI	CCRYGG	2 cut(s)	326, 996
BtgZI	GCGATG	4 cut(s)	138, 299, 739, 811
BtrI	CACGTC	1 cut(s)	714
BtsCI	GGATG	3 cut(s)	698, 1078, 1270
BtsI	GCAGTG	1 cut(s)	498
BtsIMutI	CAGTG	2 cut(s)	498, 745
BveI	ACCTGC	1 cut(s)	585
Cac8I	GCNNGC	6 cut(s)	59, 738, 1182, 1212, 1306, 1310
CfoI	GCGC	7 cut(s)	84, 478, 658, 738, 740, 859, 963
Cfr10I	RCCGGY	3 cut(s)	119, 425, 957
Cfr13I	GGNCC	7 cut(s)	316, 331, 576, 597, 804, 949, 1000
Cfr9I	CCCGGG	1 cut(s)	1352
CseI	GACGC	5 cut(s)	123, 281, 365, 553, 854
Csp6I	GTAC	6 cut(s)	298, 456, 481, 853, 979, 1063
CviAII	CATG	4 cut(s)	131, 916, 997, 1127
CviQI	GTAC	6 cut(s)	298, 456, 481, 853, 979, 1063
DdeI	CTNAG	5 cut(s)	190, 344, 377, 395, 585
DinI	GGCGCC	2 cut(s)	83, 477
DpnI	GATC	4 cut(s)	30, 213, 536, 900
DpnII	GATC	4 cut(s)	28, 211, 534, 898
DriI	GACNNNNNGTC	1 cut(s)	1127
EaeI	YGGCCR	1 cut(s)	1068
Eam1104I	CTCTTC	2 cut(s)	500, 890
Eam1105I	GACNNNNNGTC	1 cut(s)	1127
EarI	CTCTTC	2 cut(s)	500, 890
EciI	GGCGGA	1 cut(s)	1045
Eco130I	CCWWGG	1 cut(s)	996
Eco47I	GGWCC	6 cut(s)	331, 576, 597, 804, 949, 1000
Eco57I	CTGAAG	2 cut(s)	524, 657
Eco72I	CACGTG	1 cut(s)	1032
Eco88I	CYCGRG	3 cut(s)	1016, 1147, 1352
EcoNI	CCTNNNNNAGG	1 cut(s)	311
EcoO109I	RGGNCCY	2 cut(s)	331, 576
EcoRII	CCWGG	5 cut(s)	309, 684, 888, 1065, 1388
EcoT14I	CCWWGG	1 cut(s)	996
EgeI	GGCGCC	2 cut(s)	83, 477
EheI	GGCGCC	2 cut(s)	83, 477
ErhI	CCWWGG	1 cut(s)	996
Esp3I	CGTCTC	1 cut(s)	899
FaeI	CATG	4 cut(s)	134, 919, 1000, 1130
FaiI	YATR	6 cut(s)	132, 917, 998, 1128, 1281, 1283
FaqI	GGGAC	5 cut(s)	344, 562, 764, 817, 1335
FatI	CATG	4 cut(s)	130, 915, 996, 1126
FauI	CCCGC	6 cut(s)	252, 422, 442, 1236, 1297, 1301
FblI	GTMKAC	1 cut(s)	221
FokI	GGATG	3 cut(s)	685, 1065, 1257
GlaI	GCGC	7 cut(s)	83, 477, 657, 737, 739, 858, 962
GsaI	CCCAGC	1 cut(s)	207
GsuI	CTGGAG	2 cut(s)	293, 636
HaeII	RGCGCY	3 cut(s)	85, 479, 659
HaeIII	GGCC	4 cut(s)	317, 995, 1070, 1388
HgaI	GACGC	5 cut(s)	123, 281, 365, 553, 854
HhaI	GCGC	7 cut(s)	84, 478, 658, 738, 740, 859, 963
Hin1I	GRCGYC	6 cut(s)	82, 273, 476, 564, 815, 846
Hin1II	CATG	4 cut(s)	134, 919, 1000, 1130
Hin6I	GCGC	7 cut(s)	82, 476, 656, 736, 738, 857, 961
HinP1I	GCGC	7 cut(s)	82, 476, 656, 736, 738, 857, 961
HincII	GTYRAC	1 cut(s)	222
HindII	GTYRAC	1 cut(s)	222
HindIII	AAGCTT	1 cut(s)	1361
HinfI	GANTC	8 cut(s)	34, 89, 98, 894, 1021, 1094, 1237, 1252
HphI	GGTGA	4 cut(s)	383, 526, 700, 718
Hpy166II	GTNNAC	3 cut(s)	222, 496, 711
Hpy188I	TCNGA	3 cut(s)	28, 33, 633
Hpy188III	TCNNGA	7 cut(s)	532, 538, 606, 802, 902, 1018, 1042
Hpy8I	GTNNAC	3 cut(s)	222, 496, 711
HpyAV	CCTTC	5 cut(s)	286, 408, 583, 1130, 1180
HpyCH4III	ACNGT	3 cut(s)	182, 799, 923
HpyCH4IV	ACGT	6 cut(s)	300, 458, 713, 815, 908, 1031
HpyCH4V	TGCA	5 cut(s)	109, 522, 644, 862, 1204
HpyF10VI	GCNNNNNNNGC	9 cut(s)	281, 356, 359, 653, 788, 854, 1114, 1207, 1228
HpyF3I	CTNAG	5 cut(s)	190, 344, 377, 395, 585
HpySE526I	ACGT	6 cut(s)	300, 458, 713, 815, 908, 1031
Hsp92I	GRCGYC	6 cut(s)	82, 273, 476, 564, 815, 846
Hsp92II	CATG	4 cut(s)	134, 919, 1000, 1130
HspAI	GCGC	7 cut(s)	82, 476, 656, 736, 738, 857, 961
KasI	GGCGCC	2 cut(s)	81, 475
KflI	GGGWCCC	2 cut(s)	331, 576
KpnI	GGTACC	1 cut(s)	982
Kzo9I	GATC	4 cut(s)	28, 211, 534, 898
LguI	GCTCTTC	1 cut(s)	890
LmnI	GCTCC	8 cut(s)	66, 655, 661, 726, 1058, 1114, 1152, 1259
LweI	GCATC	2 cut(s)	118, 707
MaeII	ACGT	6 cut(s)	300, 458, 713, 815, 908, 1031
MalI	GATC	4 cut(s)	30, 213, 536, 900
MbiI	CCGCTC	1 cut(s)	1262
MboI	GATC	4 cut(s)	28, 211, 534, 898
MboII	GAAGA	8 cut(s)	167, 173, 301, 423, 517, 621, 810, 877
MfeI	CAATTG	1 cut(s)	774
MflI	RGATCY	2 cut(s)	28, 534
MhlI	GDGCHC	2 cut(s)	1110, 1146
MluCI	AATT	4 cut(s)	19, 774, 1169, 1411
Mly113I	GGCGCC	2 cut(s)	82, 476
MlyI	GAGTC	3 cut(s)	1103, 1246, 1261
MroXI	GAANNNNTTC	2 cut(s)	13, 884
MseI	TTAA	4 cut(s)	198, 1359, 1383, 1414
MslI	CAYNNNNRTG	1 cut(s)	731
MspA1I	CMGCKG	1 cut(s)	647
MunI	CAATTG	1 cut(s)	774
MvaI	CCWGG	5 cut(s)	311, 686, 890, 1067, 1390
MvnI	CGCG	5 cut(s)	738, 857, 877, 963, 984
MwoI	GCNNNNNNNGC	9 cut(s)	281, 356, 359, 653, 788, 854, 1114, 1207, 1228
NarI	GGCGCC	2 cut(s)	82, 476
NciI	CCSGG	7 cut(s)	79, 335, 947, 976, 1275, 1353, 1354
NcoI	CCATGG	1 cut(s)	996
NdeII	GATC	4 cut(s)	28, 211, 534, 898
NlaIII	CATG	4 cut(s)	134, 919, 1000, 1130
NmeAIII	GCCGAG	1 cut(s)	1260
NspI	RCATGY	1 cut(s)	1130
NspV	TTCGAA	2 cut(s)	17, 409
PauI	GCGCGC	1 cut(s)	736
PciI	ACATGT	1 cut(s)	1126
PciSI	GCTCTTC	1 cut(s)	890
PcsI	WCGNNNNNNNCGW	2 cut(s)	93, 818
PdmI	GAANNNNTTC	2 cut(s)	13, 884
PfeI	GAWTC	5 cut(s)	34, 89, 98, 894, 1021
Pfl23II	CGTACG	2 cut(s)	297, 480
PfoI	TCCNGGA	1 cut(s)	309
PleI	GAGTC	3 cut(s)	1102, 1245, 1260
PluTI	GGCGCC	2 cut(s)	85, 479
PmaCI	CACGTG	1 cut(s)	1032
PmlI	CACGTG	1 cut(s)	1032
PpsI	GAGTC	3 cut(s)	1102, 1245, 1260
Ppu21I	YACGTR	1 cut(s)	1032
PpuMI	RGGWCCY	2 cut(s)	331, 576
PscI	ACATGT	1 cut(s)	1126
Psp5II	RGGWCCY	2 cut(s)	331, 576
Psp6I	CCWGG	5 cut(s)	309, 684, 888, 1065, 1388
PspCI	CACGTG	1 cut(s)	1032
PspFI	CCCAGC	1 cut(s)	203
PspGI	CCWGG	5 cut(s)	309, 684, 888, 1065, 1388
PspLI	CGTACG	2 cut(s)	297, 480
PspPI	GGNCC	7 cut(s)	316, 331, 576, 597, 804, 949, 1000
PspPPI	RGGWCCY	2 cut(s)	331, 576
PstI	CTGCAG	1 cut(s)	864
PsuI	RGATCY	2 cut(s)	28, 534
PteI	GCGCGC	1 cut(s)	736
RsaI	GTAC	6 cut(s)	299, 457, 482, 854, 980, 1064
RsaNI	GTAC	6 cut(s)	298, 456, 481, 853, 979, 1063
RseI	CAYNNNNRTG	1 cut(s)	731
SalI	GTCGAC	1 cut(s)	220
SapI	GCTCTTC	1 cut(s)	890
SaqAI	TTAA	4 cut(s)	198, 1359, 1383, 1414
Sau3AI	GATC	4 cut(s)	28, 211, 534, 898
Sau96I	GGNCC	7 cut(s)	316, 331, 576, 597, 804, 949, 1000
SchI	GAGTC	3 cut(s)	1103, 1246, 1261
SduI	GDGCHC	2 cut(s)	1110, 1146
SfaNI	GCATC	2 cut(s)	118, 707
SfcI	CTRYAG	1 cut(s)	860
SfoI	GGCGCC	2 cut(s)	83, 477
SfuI	TTCGAA	2 cut(s)	17, 409
SgrAI	CRCCGGYG	2 cut(s)	119, 957
SinI	GGWCC	6 cut(s)	331, 576, 597, 804, 949, 1000
SmaI	CCCGGG	1 cut(s)	1354
SmiMI	CAYNNNNRTG	1 cut(s)	731
SmlI	CTYRAG	1 cut(s)	545
SmoI	CTYRAG	1 cut(s)	545
Sse9I	AATT	4 cut(s)	19, 774, 1169, 1411
SspDI	GGCGCC	2 cut(s)	81, 475
StyI	CCWWGG	1 cut(s)	996
TaaI	ACNGT	3 cut(s)	182, 799, 923
TaiI	ACGT	6 cut(s)	303, 461, 716, 818, 911, 1034
TaqII	GACCGA	1 cut(s)	617
TasI	AATT	4 cut(s)	19, 774, 1169, 1411
TfiI	GAWTC	5 cut(s)	34, 89, 98, 894, 1021
Tru1I	TTAA	4 cut(s)	198, 1359, 1383, 1414
Tru9I	TTAA	4 cut(s)	198, 1359, 1383, 1414
TscAI	CASTG	2 cut(s)	498, 752
TspDTI	ATGAA	3 cut(s)	183, 302, 1259
TspGWI	ACGGA	3 cut(s)	159, 589, 1308
TspMI	CCCGGG	1 cut(s)	1352
TspRI	CASTG	2 cut(s)	498, 752
VpaK11BI	GGWCC	6 cut(s)	331, 576, 597, 804, 949, 1000
XagI	CCTNNNNNAGG	1 cut(s)	311
XapI	RAATTY	2 cut(s)	19, 1169
XceI	RCATGY	1 cut(s)	1130
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1396
XmaI	CCCGGG	1 cut(s)	1352
XmiI	GTMKAC	1 cut(s)	221
XmnI	GAANNNNTTC	2 cut(s)	13, 884
ZraI	GACGTC	1 cut(s)	816

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.