Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0002215

Physical Location & Seq

Genomic Coordinates Reverse (-)

76707309 .. 76709853

Gene Structure 2545 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG446400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGGCGGCAAAGGTTTCGAATTTCTCGGATTTGGTTCAGAGGGTGGCCGCTTCTTGCTTGCTGCACCCACTTGGCGCCGGCAGGAACGGTGACTTCGCCGTGAATGGCGCCGGAGATGAGGATGATTTCAGTGAGCTCGAGTTGGATGAGCCGGAGGAGGAAGTGAGAGAAGAGGAGGAGATTGGTGGTGGAGGAGGGTTTAGGGTTAGGGAGGAGGGGTCAAGGAAGGAGGTAATGGAGATGGAGATGGCAATGTGGGAAGTGTTTGACGCCGTTTCAGCAATGAAGAAGGCCTACGCTGGCCTGCAGAAGGCGCATAGCCCTTGGGACCCGGAGAAGATAAGGGTGGCCGACATTGCGGTGGTGGGGGAGCTGAGGAAGCTTGGAGTGTTGAGGGAGAGGTTTAGGAGGAGATGTTCTGGCGGTGGAGGTCATAGAGGTGGAAGAGCGGTGGCTGGGGGTACACTGAGGGAGGTGGTGGCACCATATGAGGCGACCGTGGAGGAGCTGAAGAAGGAGCTGAAGGCTAAGGAAGTAGAGGTTGATAACCTCAAGGAAAAGCTCAGTAGTGTAGTTAATGGCTCCAACGGGAATAATGGCAAGAAGGGCAGATCTATCTCAAGGAGGAAGGTAGGTTGCAGTCAACTTCAAGTTGTAGCTGCTCCAGCACCGGATCTGTTTGAGGCAACAATGAGCCAAGTGAAAGAGGCATCAAAGTCCTTCACTTTATTGCTCCTCTCTCTCATGCGTGCCGCCCATTGGGACATTGCCGCAGCTGTACGTTCCATTGAAGCTGCCACTGTCACTGCCATTACCACAACAGCTATTAACACATCCTCCATTCTCACAACGACCCAGAATGGCAGTGCCAAGTATGCTCTCCAGTCCTACATTTCCAGCAAAATGTTCCAAGGCTTTGATCACGAGACATTCTACATGGACGGTAGCCTCTCCTCGCTTCTCAACCCAGATCAGTACCGCCGTGATTGCTTCACTCAATACCGTGACATGAAGGCCATGGATCCTGCCGAGCTTCTTGGAATATTGCCCACGTGTCACTTTGGCAAGTTCTGCTCAAACAGGTACTTGGCCATAGTTCATCCCAAGATGGAAGAGTCATTGTTCGGAGACTTGGATCAGCGACGCCAGGTCTTAGATGGAAACCACCCCAGGAGTGAGTTTTACAGTCAGTTCTTGGGGATGGCTAAAGCAGTCTGGTTACTGCACTTGCTGGCCTTCTCTCTTGACCCAACACCTAGTCAATTTGAGGCTACTAGAGGTGCTGAGTTTCATCCACACTACATGGAAAGTGTTGTCAAGTTTTCGGGTGGGCGGGTTCCTTCGGGTCAGGTTGTCGGGTTTCCTGTAAGTCCTGGGTTCAAGCTTGGAAATGGGTCTATTATCAAGGCCAGAGTTTACCTAGTTTCGAGGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009639 GO:0009959 GO:0050896

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF641

Protein Analysis

478

Amino Acids

52.51

Weight (kDa)

6.45

Isoelectric Point (pI)

45.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF641	PF04859	77 - 194	4.5e-34	Plant protein of unknown function (DUF641)
GIL1_IRKI_C	PF24994	422 - 473	2.2e-10	GIL1/IRKI C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002215)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58960 AT5G58960 AT5G58960
fragaria_vesca	FvH4_3g37820 FvH4_3g37850
malus_domestica	MD03G1078100.v1.1 MD11G1082800.v1.1
prunus_persica	Prupe.6G063100_v2.0.a1
pyrus_communis	pycom03g06240 pycom11g07090
rosa_chinensis	RchiOBHm_Chr5g0068001 RchiOBHm_Chr5g0068051 RchiOBHm_Chr5g0068071
rosa_laevigata	RLG00000035951 RLG00000035952
rosa_multiflora	Rmu_co8216896.1_g000001 Rmu_co8424771.1_g000001 Rmu_sc0000435.1_g000019 Rmu_sc0000435.1_g000025 Rmu_sc0000435.1_g000034
rosa_roxburghii	Rroxscaffold_1G00012550 Rroxscaffold_1G00012560 Rroxscaffold_1G00013280 Rroxscaffold_1G00013290
rosa_rugosa	Rorug05G0387200 Rorug05G0387600 Rorug05G0388100
rosa_samantha	Rh5AG446100 Rh5AG446300 Rh5AG446400 Rh5BG463600 Rh5BG464000 Rh5BG464300 Rh5CG485500 Rh5CG486000 Rh5CG486300 Rh5DG477600 Rh5DG478000 Rh5DG478300
rosa_wichuraiana	Rw5G041670 Rw5G041680 Rw5G041710 Rw5G041720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	74, 107, 481
AccBSI	CCGCTC	1 cut(s)	449
AciI	CCGC	9 cut(s)	5, 48, 359, 423, 449, 753, 771, 979, 1333
AclWI	GGATC	4 cut(s)	681, 1016, 1029, 1143
AcoI	YGGCCR	3 cut(s)	45, 348, 1089
AcsI	RAATTY	1 cut(s)	19
AcuI	CTGAAG	2 cut(s)	530, 542
AcvI	CACGTG	1 cut(s)	1053
AcyI	GRCGYC	4 cut(s)	75, 108, 270, 1144
AfaI	GTAC	4 cut(s)	463, 780, 977, 1085
AfiI	CCNNNNNNNGG	2 cut(s)	310, 759
AflIII	ACRYGT	1 cut(s)	1052
AgsI	TTSAA	3 cut(s)	650, 791, 1381
AhdI	GACNNNNNGTC	1 cut(s)	1148
AjnI	CCWGG	3 cut(s)	1146, 1169, 1372
AloI	GAACNNNNNNTCC	2 cut(s)	400, 432
Alw21I	GWGCWC	1 cut(s)	138
Alw26I	GTCTC	2 cut(s)	920, 1122
AlwI	GGATC	4 cut(s)	681, 1016, 1029, 1143
Ama87I	CYCGRG	1 cut(s)	137
AoxI	GGCC	8 cut(s)	45, 291, 301, 348, 1014, 1089, 1233, 1407
ApeKI	GCWGC	4 cut(s)	61, 659, 773, 794
ApoI	RAATTY	1 cut(s)	19
AspLEI	GCGC	3 cut(s)	77, 110, 316
AspS9I	GGNCC	1 cut(s)	328
AsuC2I	CCSGG	1 cut(s)	332
AsuHPI	GGTGA	1 cut(s)	101
AsuII	TTCGAA	1 cut(s)	17
AvaI	CYCGRG	1 cut(s)	137
AvaII	GGWCC	1 cut(s)	328
BalI	TGGCCA	1 cut(s)	1091
BamHI	GGATCC	1 cut(s)	1021
BanI	GGYRCC	3 cut(s)	74, 107, 481
BanII	GRGCYC	1 cut(s)	138
BarI	GAAGNNNNNNTAC	2 cut(s)	278, 310
BauI	CACGAG	1 cut(s)	923
BbrPI	CACGTG	1 cut(s)	1053
Bbv12I	GWGCWC	1 cut(s)	138
BbvCI	CCTCAGC	1 cut(s)	374
BbvI	GCAGC	4 cut(s)	48, 646, 781, 785
BccI	CCATC	5 cut(s)	235, 241, 1102, 1151, 1195
BceAI	ACGGC	3 cut(s)	83, 257, 966
BcgI	CGANNNNNNTGC	1 cut(s)	31
BciT130I	CCWGG	3 cut(s)	1148, 1171, 1374
BclI	TGATCA	1 cut(s)	919
BcnI	CCSGG	1 cut(s)	332
BcoDI	GTCTC	2 cut(s)	920, 1122
BfaI	CTAG	3 cut(s)	1257, 1275, 1421
BfmI	CTRYAG	1 cut(s)	305
BfoI	RGCGCY	2 cut(s)	78, 111
BglII	AGATCT	1 cut(s)	611
BisI	GCNGC	8 cut(s)	6, 48, 62, 660, 753, 771, 774, 795
BlsI	GCNGC	8 cut(s)	7, 49, 63, 661, 754, 772, 775, 796
Bme1390I	CCNGG	4 cut(s)	332, 1148, 1171, 1374
Bme18I	GGWCC	1 cut(s)	328
BmeRI	GACNNNNNGTC	1 cut(s)	1148
BmeT110I	CYCGRG	1 cut(s)	137
BmgT120I	GGNCC	1 cut(s)	328
BmiI	GGNNCC	8 cut(s)	76, 109, 329, 330, 483, 583, 1023, 1338
BmrFI	CCNGG	4 cut(s)	332, 1148, 1171, 1374
BmsI	GCATC	1 cut(s)	719
BpmI	CTGGAG	2 cut(s)	648, 866
Bpu10I	CCTNAGC	2 cut(s)	374, 528
Bpu14I	TTCGAA	1 cut(s)	17
BpuEI	CTTGAG	2 cut(s)	536, 604
BpuMI	CCSGG	1 cut(s)	332
BsaAI	YACGTR	1 cut(s)	1053
BsaHI	GRCGYC	4 cut(s)	75, 108, 270, 1144
BsaJI	CCNNGG	6 cut(s)	323, 498, 910, 1017, 1169, 1373
BsaWI	WCCGGW	1 cut(s)	670
BsaXI	ACNNNNNCTCC	6 cut(s)	203, 233, 400, 430, 821, 851
Bsc4I	CCNNNNNNNGG	2 cut(s)	310, 759
Bse118I	RCCGGY	1 cut(s)	77
Bse1I	ACTGG	1 cut(s)	883
Bse3DI	GCAATG	4 cut(s)	258, 288, 354, 765
BseBI	CCWGG	3 cut(s)	1148, 1171, 1374
BseDI	CCNNGG	6 cut(s)	323, 498, 910, 1017, 1169, 1373
BseGI	GGATG	6 cut(s)	127, 151, 833, 1099, 1206, 1291
BseLI	CCNNNNNNNGG	2 cut(s)	310, 759
BseMI	GCAATG	4 cut(s)	258, 288, 354, 765
BseMII	CTCAG	4 cut(s)	365, 458, 577, 1275
BseNI	ACTGG	1 cut(s)	883
BseRI	GAGGAG	9 cut(s)	170, 188, 191, 207, 227, 424, 518, 725, 943
BseXI	GCAGC	4 cut(s)	48, 646, 781, 785
BseYI	CCCAGC	1 cut(s)	455
BsgI	GTGCAG	2 cut(s)	47, 1208
Bsh1285I	CGRYCG	1 cut(s)	498
BshFI	GGCC	8 cut(s)	47, 293, 303, 350, 1016, 1091, 1235, 1409
BshNI	GGYRCC	3 cut(s)	74, 107, 481
BsiEI	CGRYCG	1 cut(s)	498
BsiHKAI	GWGCWC	1 cut(s)	138
BsiHKCI	CYCGRG	1 cut(s)	137
BsiSI	CCGG	5 cut(s)	78, 111, 152, 332, 671
BslFI	GGGAC	2 cut(s)	341, 776
BslI	CCNNNNNNNGG	2 cut(s)	310, 759
BsmAI	GTCTC	2 cut(s)	920, 1122
BsmFI	GGGAC	2 cut(s)	341, 776
BsnI	GGCC	8 cut(s)	47, 293, 303, 350, 1016, 1091, 1235, 1409
BsoBI	CYCGRG	1 cut(s)	137
Bsp119I	TTCGAA	1 cut(s)	17
Bsp1286I	GDGCHC	1 cut(s)	138
Bsp143I	GATC	6 cut(s)	611, 673, 919, 970, 1021, 1135
Bsp19I	CCATGG	1 cut(s)	1017
BspACI	CCGC	9 cut(s)	5, 48, 359, 423, 449, 753, 771, 979, 1333
BspANI	GGCC	8 cut(s)	47, 293, 303, 350, 1016, 1091, 1235, 1409
BspCNI	CTCAG	4 cut(s)	366, 459, 576, 1276
BspLI	GGNNCC	8 cut(s)	76, 109, 329, 330, 483, 583, 1023, 1338
BspMAI	CTGCAG	1 cut(s)	309
BspPI	GGATC	4 cut(s)	681, 1016, 1029, 1143
BspQI	GCTCTTC	1 cut(s)	439
BspT104I	TTCGAA	1 cut(s)	17
BspT107I	GGYRCC	3 cut(s)	74, 107, 481
BsrBI	CCGCTC	1 cut(s)	449
BsrDI	GCAATG	4 cut(s)	258, 288, 354, 765
BsrFI	RCCGGY	1 cut(s)	77
BsrI	ACTGG	1 cut(s)	883
BssAI	RCCGGY	1 cut(s)	77
BssECI	CCNNGG	6 cut(s)	323, 498, 910, 1017, 1169, 1373
BssMI	GATC	6 cut(s)	611, 673, 919, 970, 1021, 1135
BssNI	GRCGYC	4 cut(s)	75, 108, 270, 1144
BssSI	CACGAG	1 cut(s)	923
BssT1I	CCWWGG	3 cut(s)	323, 910, 1017
Bst2BI	CACGAG	1 cut(s)	923
Bst2UI	CCWGG	3 cut(s)	1148, 1171, 1374
Bst4CI	ACNGT	6 cut(s)	89, 499, 802, 944, 1004, 1187
Bst6I	CTCTTC	3 cut(s)	165, 439, 1107
BstACI	GRCGYC	4 cut(s)	75, 108, 270, 1144
BstAPI	GCANNNNNTGC	1 cut(s)	1071
BstBAI	YACGTR	1 cut(s)	1053
BstBI	TTCGAA	1 cut(s)	17
BstC8I	GCNNGC	6 cut(s)	59, 79, 301, 305, 750, 1233
BstDEI	CTNAG	6 cut(s)	374, 467, 528, 563, 1153, 1284
BstDSI	CCRYGG	2 cut(s)	498, 1017
BstENI	CCTNNNNNAGG	1 cut(s)	308
BstF5I	GGATG	6 cut(s)	127, 151, 833, 1099, 1206, 1291
BstH2I	RGCGCY	2 cut(s)	78, 111
BstHHI	GCGC	3 cut(s)	77, 110, 316
BstKTI	GATC	6 cut(s)	614, 676, 922, 973, 1024, 1138
BstMAI	GTCTC	2 cut(s)	920, 1122
BstMBI	GATC	6 cut(s)	611, 673, 919, 970, 1021, 1135
BstMCI	CGRYCG	1 cut(s)	498
BstMWI	GCNNNNNNNGC	9 cut(s)	278, 313, 356, 379, 606, 665, 875, 987, 1071
BstNI	CCWGG	3 cut(s)	1148, 1171, 1374
BstSCI	CCNGG	4 cut(s)	330, 1146, 1169, 1372
BstSFI	CTRYAG	1 cut(s)	305
BstV1I	GCAGC	4 cut(s)	48, 646, 781, 785
BstX2I	RGATCY	3 cut(s)	611, 673, 1021
BstYI	RGATCY	3 cut(s)	611, 673, 1021
BsuRI	GGCC	8 cut(s)	47, 293, 303, 350, 1016, 1091, 1235, 1409
BtgI	CCRYGG	2 cut(s)	498, 1017
BtsCI	GGATG	6 cut(s)	127, 151, 833, 1099, 1206, 1291
BtsI	GCAGTG	2 cut(s)	804, 871
BtsIMutI	CAGTG	5 cut(s)	136, 464, 798, 804, 871
Cac8I	GCNNGC	6 cut(s)	59, 79, 301, 305, 750, 1233
CfoI	GCGC	3 cut(s)	77, 110, 316
Cfr10I	RCCGGY	1 cut(s)	77
Cfr13I	GGNCC	1 cut(s)	328
CseI	GACGC	2 cut(s)	278, 1152
Csp6I	GTAC	4 cut(s)	462, 779, 976, 1084
CviAII	CATG	5 cut(s)	745, 937, 1009, 1018, 1303
CviQI	GTAC	4 cut(s)	462, 779, 976, 1084
DdeI	CTNAG	6 cut(s)	374, 467, 528, 563, 1153, 1284
DinI	GGCGCC	2 cut(s)	76, 109
DpnI	GATC	6 cut(s)	613, 675, 921, 972, 1023, 1137
DpnII	GATC	6 cut(s)	611, 673, 919, 970, 1021, 1135
DriI	GACNNNNNGTC	1 cut(s)	1148
EaeI	YGGCCR	3 cut(s)	45, 348, 1089
Eam1104I	CTCTTC	3 cut(s)	165, 439, 1107
Eam1105I	GACNNNNNGTC	1 cut(s)	1148
EarI	CTCTTC	3 cut(s)	165, 439, 1107
Ecl136II	GAGCTC	1 cut(s)	136
Eco130I	CCWWGG	3 cut(s)	323, 910, 1017
Eco147I	AGGCCT	1 cut(s)	293
Eco24I	GRGCYC	1 cut(s)	138
Eco47I	GGWCC	1 cut(s)	328
Eco53kI	GAGCTC	1 cut(s)	136
Eco57I	CTGAAG	2 cut(s)	530, 542
Eco72I	CACGTG	1 cut(s)	1053
Eco88I	CYCGRG	1 cut(s)	137
EcoICRI	GAGCTC	1 cut(s)	136
EcoNI	CCTNNNNNAGG	1 cut(s)	308
EcoO109I	RGGNCCY	1 cut(s)	328
EcoRII	CCWGG	3 cut(s)	1146, 1169, 1372
EcoT14I	CCWWGG	3 cut(s)	323, 910, 1017
EcoT38I	GRGCYC	1 cut(s)	138
EgeI	GGCGCC	2 cut(s)	76, 109
EheI	GGCGCC	2 cut(s)	76, 109
ErhI	CCWWGG	3 cut(s)	323, 910, 1017
FaeI	CATG	5 cut(s)	748, 940, 1012, 1021, 1306
FaqI	GGGAC	2 cut(s)	341, 776
FatI	CATG	5 cut(s)	744, 936, 1008, 1017, 1302
FauI	CCCGC	1 cut(s)	1326
FauNDI	CATATG	1 cut(s)	487
FbaI	TGATCA	1 cut(s)	919
Fnu4HI	GCNGC	8 cut(s)	6, 48, 62, 660, 753, 771, 774, 795
FokI	GGATG	6 cut(s)	134, 158, 820, 1086, 1213, 1278
FriOI	GRGCYC	1 cut(s)	138
Fsp4HI	GCNGC	8 cut(s)	6, 48, 62, 660, 753, 771, 774, 795
FspBI	CTAG	3 cut(s)	1257, 1275, 1421
GlaI	GCGC	3 cut(s)	76, 109, 315
GluI	GCNGC	8 cut(s)	6, 48, 62, 660, 753, 771, 774, 795
GsaI	CCCAGC	1 cut(s)	459
GsuI	CTGGAG	2 cut(s)	648, 866
HaeII	RGCGCY	2 cut(s)	78, 111
HaeIII	GGCC	8 cut(s)	47, 293, 303, 350, 1016, 1091, 1235, 1409
HapII	CCGG	5 cut(s)	78, 111, 152, 332, 671
HgaI	GACGC	2 cut(s)	278, 1152
HhaI	GCGC	3 cut(s)	77, 110, 316
Hin1I	GRCGYC	4 cut(s)	75, 108, 270, 1144
Hin1II	CATG	5 cut(s)	748, 940, 1012, 1021, 1306
Hin6I	GCGC	3 cut(s)	75, 108, 314
HinP1I	GCGC	3 cut(s)	75, 108, 314
HincII	GTYRAC	1 cut(s)	644
HindII	GTYRAC	1 cut(s)	644
HindIII	AAGCTT	2 cut(s)	380, 1382
HinfI	GANTC	1 cut(s)	1115
HpaII	CCGG	5 cut(s)	78, 111, 152, 332, 671
HphI	GGTGA	1 cut(s)	101
Hpy166II	GTNNAC	3 cut(s)	464, 644, 1417
Hpy188I	TCNGA	3 cut(s)	28, 39, 1127
Hpy188III	TCNNGA	2 cut(s)	923, 1244
Hpy8I	GTNNAC	3 cut(s)	464, 644, 1417
Hpy99I	CGWCG	1 cut(s)	1146
HpyCH4III	ACNGT	6 cut(s)	89, 499, 802, 944, 1004, 1187
HpyCH4IV	ACGT	2 cut(s)	781, 1052
HpyCH4V	TGCA	4 cut(s)	64, 307, 639, 1225
HpyF10VI	GCNNNNNNNGC	9 cut(s)	278, 313, 356, 379, 606, 665, 875, 987, 1071
HpyF3I	CTNAG	6 cut(s)	374, 467, 528, 563, 1153, 1284
HpySE526I	ACGT	2 cut(s)	781, 1052
Hsp92I	GRCGYC	4 cut(s)	75, 108, 270, 1144
Hsp92II	CATG	5 cut(s)	748, 940, 1012, 1021, 1306
HspAI	GCGC	3 cut(s)	75, 108, 314
KasI	GGCGCC	2 cut(s)	74, 107
KflI	GGGWCCC	1 cut(s)	328
KroI	GCCGGC	1 cut(s)	77
KroNI	GCCGGC	1 cut(s)	79
Ksp22I	TGATCA	1 cut(s)	919
Kzo9I	GATC	6 cut(s)	611, 673, 919, 970, 1021, 1135
LguI	GCTCTTC	1 cut(s)	439
LmnI	GCTCC	6 cut(s)	370, 505, 517, 587, 667, 738
Lsp1109I	GCAGC	4 cut(s)	48, 646, 781, 785
LweI	GCATC	1 cut(s)	719
MaeI	CTAG	3 cut(s)	1257, 1275, 1421
MaeII	ACGT	2 cut(s)	781, 1052
MaeIII	GTNAC	5 cut(s)	89, 802, 1004, 1055, 1218
MalI	GATC	6 cut(s)	613, 675, 921, 972, 1023, 1137
MbiI	CCGCTC	1 cut(s)	449
MboI	GATC	6 cut(s)	611, 673, 919, 970, 1021, 1135
MboII	GAAGA	6 cut(s)	182, 298, 349, 456, 523, 1124
MflI	RGATCY	3 cut(s)	611, 673, 1021
MhlI	GDGCHC	1 cut(s)	138
MlsI	TGGCCA	1 cut(s)	1091
MluCI	AATT	2 cut(s)	19, 1262
MluNI	TGGCCA	1 cut(s)	1091
Mly113I	GGCGCC	2 cut(s)	75, 108
MlyI	GAGTC	1 cut(s)	1124
MmeI	TCCRAC	2 cut(s)	123, 609
Mox20I	TGGCCA	1 cut(s)	1091
MroNI	GCCGGC	1 cut(s)	77
MscI	TGGCCA	1 cut(s)	1091
MseI	TTAA	2 cut(s)	576, 828
MslI	CAYNNNNRTG	2 cut(s)	359, 438
Msp20I	TGGCCA	1 cut(s)	1091
MspA1I	CMGCKG	1 cut(s)	776
MspI	CCGG	5 cut(s)	78, 111, 152, 332, 671
MspR9I	CCNGG	4 cut(s)	332, 1148, 1171, 1374
MvaI	CCWGG	3 cut(s)	1148, 1171, 1374
MwoI	GCNNNNNNNGC	9 cut(s)	278, 313, 356, 379, 606, 665, 875, 987, 1071
NaeI	GCCGGC	1 cut(s)	79
NarI	GGCGCC	2 cut(s)	75, 108
NciI	CCSGG	1 cut(s)	332
NcoI	CCATGG	1 cut(s)	1017
NdeI	CATATG	1 cut(s)	487
NdeII	GATC	6 cut(s)	611, 673, 919, 970, 1021, 1135
NgoMIV	GCCGGC	1 cut(s)	77
NlaIII	CATG	5 cut(s)	748, 940, 1012, 1021, 1306
NlaIV	GGNNCC	8 cut(s)	76, 109, 329, 330, 483, 583, 1023, 1338
NmeAIII	GCCGAG	1 cut(s)	1054
NmuCI	GTSAC	4 cut(s)	89, 802, 1004, 1055
NspV	TTCGAA	1 cut(s)	17
PaeR7I	CTCGAG	1 cut(s)	137
PceI	AGGCCT	1 cut(s)	293
PciSI	GCTCTTC	1 cut(s)	439
PdiI	GCCGGC	1 cut(s)	79
PkrI	GCNGC	8 cut(s)	7, 49, 63, 661, 754, 772, 775, 796
PleI	GAGTC	1 cut(s)	1123
PluTI	GGCGCC	2 cut(s)	78, 111
PmaCI	CACGTG	1 cut(s)	1053
PmlI	CACGTG	1 cut(s)	1053
PpsI	GAGTC	1 cut(s)	1123
Ppu21I	YACGTR	1 cut(s)	1053
PpuMI	RGGWCCY	1 cut(s)	328
Psp124BI	GAGCTC	1 cut(s)	138
Psp5II	RGGWCCY	1 cut(s)	328
Psp6I	CCWGG	3 cut(s)	1146, 1169, 1372
PspCI	CACGTG	1 cut(s)	1053
PspFI	CCCAGC	1 cut(s)	455
PspGI	CCWGG	3 cut(s)	1146, 1169, 1372
PspN4I	GGNNCC	8 cut(s)	76, 109, 329, 330, 483, 583, 1023, 1338
PspPI	GGNCC	1 cut(s)	328
PspPPI	RGGWCCY	1 cut(s)	328
PspXI	VCTCGAGB	1 cut(s)	137
PstI	CTGCAG	1 cut(s)	309
PsuI	RGATCY	3 cut(s)	611, 673, 1021
PvuII	CAGCTG	1 cut(s)	776
RsaI	GTAC	4 cut(s)	463, 780, 977, 1085
RsaNI	GTAC	4 cut(s)	462, 779, 976, 1084
RseI	CAYNNNNRTG	2 cut(s)	359, 438
SacI	GAGCTC	1 cut(s)	138
SapI	GCTCTTC	1 cut(s)	439
SaqAI	TTAA	2 cut(s)	576, 828
SatI	GCNGC	8 cut(s)	6, 48, 62, 660, 753, 771, 774, 795
Sau3AI	GATC	6 cut(s)	611, 673, 919, 970, 1021, 1135
Sau96I	GGNCC	1 cut(s)	328
SchI	GAGTC	1 cut(s)	1124
ScrFI	CCNGG	4 cut(s)	332, 1148, 1171, 1374
SduI	GDGCHC	1 cut(s)	138
SfaNI	GCATC	1 cut(s)	719
SfcI	CTRYAG	1 cut(s)	305
SfoI	GGCGCC	2 cut(s)	76, 109
Sfr274I	CTCGAG	1 cut(s)	137
SfuI	TTCGAA	1 cut(s)	17
SinI	GGWCC	1 cut(s)	328
SlaI	CTCGAG	1 cut(s)	137
SmiMI	CAYNNNNRTG	2 cut(s)	359, 438
SmlI	CTYRAG	3 cut(s)	137, 551, 619
SmoI	CTYRAG	3 cut(s)	137, 551, 619
Sse9I	AATT	2 cut(s)	19, 1262
SseBI	AGGCCT	1 cut(s)	293
SsiI	CCGC	9 cut(s)	5, 48, 359, 423, 449, 753, 771, 979, 1333
SspDI	GGCGCC	2 cut(s)	74, 107
SspI	AATATT	1 cut(s)	1044
SspMI	CTAG	3 cut(s)	1257, 1275, 1421
SstI	GAGCTC	1 cut(s)	138
StuI	AGGCCT	1 cut(s)	293
StyD4I	CCNGG	4 cut(s)	330, 1146, 1169, 1372
StyI	CCWWGG	3 cut(s)	323, 910, 1017
TaaI	ACNGT	6 cut(s)	89, 499, 802, 944, 1004, 1187
TaiI	ACGT	2 cut(s)	784, 1055
TaqI	TCGA	3 cut(s)	17, 138, 1427
TasI	AATT	2 cut(s)	19, 1262
TauI	GCSGC	4 cut(s)	8, 50, 755, 773
Tru1I	TTAA	2 cut(s)	576, 828
Tru9I	TTAA	2 cut(s)	576, 828
TscAI	CASTG	5 cut(s)	136, 471, 805, 811, 871
TseFI	GTSAC	4 cut(s)	89, 802, 1004, 1055
TseI	GCWGC	4 cut(s)	61, 659, 773, 794
Tsp45I	GTSAC	4 cut(s)	89, 802, 1004, 1055
TspDTI	ATGAA	4 cut(s)	299, 1025, 1088, 1280
TspRI	CASTG	5 cut(s)	136, 471, 805, 811, 871
VpaK11BI	GGWCC	1 cut(s)	328
XagI	CCTNNNNNAGG	1 cut(s)	308
XapI	RAATTY	1 cut(s)	19
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1154
XhoI	CTCGAG	1 cut(s)	137
XspI	CTAG	3 cut(s)	1257, 1275, 1421

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.