Rh5DG477600

Plant protein of unknown function (DUF641)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002215
Physical Location & Seq
Reverse (-)
75429374 .. 75430105
732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG477600.1

Sequence Viewer

Length: 732 bp
ATGAGCGGAGTTAGAGAGGCATTAGACACCTTCACTATAGTTCTCCTCTCTCTCATGCGTGCGGCTCACTGGGATATCGCTGCGGCCGTACGCTCCATTGAAGCCGCCACTGCCACAGCTACGAGGATCAGCGCTTCCTCTGTAATCCAAACTCAACGTGCCAACGCCAAGTTCGCTCTTCAGTCCTACGTTTCCCGAAAAGTCTTTCAAGGCTTCGACCACGAGAGCTTCTACATGGATAACGGCCTCTCCTCAGTTCTCAGCCCAGACCAGTATCGCCGGGACTGCTTCTCCGAATATTGTGACATGAAAAGCATGGACTCCACAGAGCTTCTCAATATCCAGCCCTCGTGTAACTTTGGCAAGTTTTGCTCCAACAAGTACTTGGCTATTGTTCATCCCAAGATGGAAGAGTCATTCTTCGGAGACTTGGAGCAGAGACAACAAGTTGTAGAAGGCAGGCATCCTAGGAGTCAGTTTTACATGCAGTTCTTGGAGTTGGCTAAGGCGATGTGGTTGCTTCACTTGCTGGCGTTCTCACTTGACCCTGCACCAAGTCAGTTTGAGGCTGGCAGAGGAGCTGATTTTCATCCAGACTACATGGAGGGTGTTATAAAGTCTCCAGGACAAGTTCCTTCGGGTCAAGTTGTTGGGTTTCCCTTAATTCCAGGGTTCAAGCTTGGCAATGGGTCCATTATTAAGGCCAGAGTTTACCTTGTATCCAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009639 GO:0009959 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF641

Protein Analysis

243

Amino Acids

27.16

Weight (kDa)

7.01

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIL1_IRKI_C PF24994 188 - 238 1.3e-07 GIL1/IRKI C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 614
AccBSI CCGCTC 1 cut(s) 6
AciI CCGC 4 cut(s) 6, 62, 83, 105
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 84
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 2 cut(s) 90, 383
AfeI AGCGCT 1 cut(s) 133
AgsI TTSAA 3 cut(s) 101, 209, 676
AjnI CCWGG 2 cut(s) 622, 667
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AluBI AGCT 5 cut(s) 119, 228, 331, 581, 679
AluI AGCT 5 cut(s) 119, 228, 331, 581, 679
Alw26I GTCTC 3 cut(s) 420, 433, 624
AlwI GGATC 1 cut(s) 134
Aor51HI AGCGCT 1 cut(s) 133
AoxI GGCC 3 cut(s) 84, 244, 702
ApeKI GCWGC 1 cut(s) 80
AspA2I CCTAGG 1 cut(s) 467
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 690
AsuC2I CCSGG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 690
AvrII CCTAGG 1 cut(s) 467
BauI CACGAG 2 cut(s) 221, 349
BbvI GCAGC 1 cut(s) 67
BccI CCATC 1 cut(s) 400
BceAI ACGGC 2 cut(s) 71, 259
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BciT130I CCWGG 2 cut(s) 624, 669
BciVI GTATCC 1 cut(s) 730
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 3 cut(s) 420, 433, 624
BfaI CTAG 1 cut(s) 468
BfmI CTRYAG 1 cut(s) 36
BfoI RGCGCY 1 cut(s) 135
BfuI GTATCC 1 cut(s) 730
BisI GCNGC 4 cut(s) 63, 81, 84, 105
BlnI CCTAGG 1 cut(s) 467
BlsI GCNGC 4 cut(s) 64, 82, 85, 106
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 3 cut(s) 281, 624, 669
Bme18I GGWCC 1 cut(s) 690
BmgT120I GGNCC 1 cut(s) 690
BmiI GGNNCC 1 cut(s) 691
BmrFI CCNGG 3 cut(s) 281, 624, 669
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 1 cut(s) 472
BmuI ACTGGG 1 cut(s) 79
BpmI CTGGAG 1 cut(s) 606
Bpu10I CCTNAGC 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 281
BsaBI GATNNNNATC 1 cut(s) 588
BsaJI CCNNGG 2 cut(s) 467, 668
BsaXI ACNNNNNCTCC 4 cut(s) 233, 263, 275, 305
Bse1I ACTGG 2 cut(s) 74, 271
Bse3DI GCAATG 1 cut(s) 691
Bse8I GATNNNNATC 1 cut(s) 588
BseBI CCWGG 2 cut(s) 624, 669
BseDI CCNNGG 2 cut(s) 467, 668
BseGI GGATG 3 cut(s) 397, 463, 589
BseJI GATNNNNATC 1 cut(s) 588
BseMI GCAATG 1 cut(s) 691
BseMII CTCAG 2 cut(s) 267, 274
BseNI ACTGG 2 cut(s) 74, 271
BseRI GAGGAG 3 cut(s) 35, 241, 591
BseX3I CGGCCG 1 cut(s) 84
BseXI GCAGC 1 cut(s) 67
BsgI GTGCAG 1 cut(s) 534
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 3 cut(s) 86, 246, 704
BsiEI CGRYCG 1 cut(s) 87
BsiSI CCGG 1 cut(s) 280
BsiWI CGTACG 1 cut(s) 88
BslFI GGGAC 1 cut(s) 296
BsmAI GTCTC 3 cut(s) 420, 433, 624
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 3 cut(s) 86, 246, 704
Bsp143I GATC 1 cut(s) 126
BspACI CCGC 4 cut(s) 6, 62, 83, 105
BspANI GGCC 3 cut(s) 86, 246, 704
BspCNI CTCAG 2 cut(s) 266, 273
BspLI GGNNCC 1 cut(s) 691
BspPI GGATC 1 cut(s) 134
BspQI GCTCTTC 1 cut(s) 183
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 691
BsrI ACTGG 2 cut(s) 74, 271
BssECI CCNNGG 2 cut(s) 467, 668
BssMI GATC 1 cut(s) 126
BssSI CACGAG 2 cut(s) 221, 349
BssT1I CCWWGG 1 cut(s) 467
Bst2BI CACGAG 2 cut(s) 221, 349
Bst2UI CCWGG 2 cut(s) 624, 669
Bst6I CTCTTC 2 cut(s) 183, 405
BstAPI GCANNNNNTGC 1 cut(s) 369
BstC8I GCNNGC 4 cut(s) 60, 461, 531, 571
BstDEI CTNAG 3 cut(s) 253, 260, 504
BstF5I GGATG 3 cut(s) 397, 463, 589
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 3 cut(s) 420, 433, 624
BstMBI GATC 1 cut(s) 126
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 5 cut(s) 110, 173, 285, 369, 526
BstNI CCWGG 2 cut(s) 624, 669
BstNSI RCATGY 1 cut(s) 487
BstSCI CCNGG 3 cut(s) 279, 622, 667
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 1 cut(s) 67
BstZI CGGCCG 1 cut(s) 84
BsuI GTATCC 1 cut(s) 730
BsuRI GGCC 3 cut(s) 86, 246, 704
BtgZI GCGATG 1 cut(s) 524
BtsCI GGATG 3 cut(s) 397, 463, 589
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 2 cut(s) 67, 108
Cac8I GCNNGC 4 cut(s) 60, 461, 531, 571
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 690
Csp6I GTAC 2 cut(s) 89, 382
CviAII CATG 6 cut(s) 55, 235, 307, 316, 484, 601
CviQI GTAC 2 cut(s) 89, 382
DdeI CTNAG 3 cut(s) 253, 260, 504
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
EaeI YGGCCR 1 cut(s) 84
EagI CGGCCG 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 183, 405
EarI CTCTTC 2 cut(s) 183, 405
EclXI CGGCCG 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 467
Eco32I GATATC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 690
Eco47III AGCGCT 1 cut(s) 133
Eco52I CGGCCG 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 622, 667
EcoRV GATATC 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 467
ErhI CCWWGG 1 cut(s) 467
FaeI CATG 6 cut(s) 58, 238, 310, 319, 487, 604
FaiI YATR 8 cut(s) 38, 56, 236, 308, 317, 485, 602, 614
FaqI GGGAC 1 cut(s) 296
FatI CATG 6 cut(s) 54, 234, 306, 315, 483, 600
Fnu4HI GCNGC 4 cut(s) 63, 81, 84, 105
FokI GGATG 3 cut(s) 384, 450, 576
Fsp4HI GCNGC 4 cut(s) 63, 81, 84, 105
FspBI CTAG 1 cut(s) 468
GlaI GCGC 1 cut(s) 133
GluI GCNGC 4 cut(s) 63, 81, 84, 105
GsuI CTGGAG 1 cut(s) 606
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 3 cut(s) 86, 246, 704
HapII CCGG 1 cut(s) 280
HhaI GCGC 1 cut(s) 134
Hin1II CATG 6 cut(s) 58, 238, 310, 319, 487, 604
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 3 cut(s) 320, 413, 472
HpaII CCGG 1 cut(s) 280
Hpy166II GTNNAC 1 cut(s) 712
Hpy188I TCNGA 2 cut(s) 295, 425
Hpy188III TCNNGA 2 cut(s) 195, 593
Hpy8I GTNNAC 1 cut(s) 712
HpyAV CCTTC 3 cut(s) 40, 449, 645
HpyCH4IV ACGT 2 cut(s) 157, 189
HpyCH4V TGCA 2 cut(s) 487, 551
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 173, 285, 369, 526
HpyF3I CTNAG 3 cut(s) 253, 260, 504
HpySE526I ACGT 2 cut(s) 157, 189
Hsp92II CATG 6 cut(s) 58, 238, 310, 319, 487, 604
HspAI GCGC 1 cut(s) 132
Kzo9I GATC 1 cut(s) 126
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 4 cut(s) 98, 377, 433, 578
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 1 cut(s) 472
MaeI CTAG 1 cut(s) 468
MaeII ACGT 2 cut(s) 157, 189
MaeIII GTNAC 2 cut(s) 302, 353
MalI GATC 1 cut(s) 128
MbiI CCGCTC 1 cut(s) 6
MboI GATC 1 cut(s) 126
MboII GAAGA 3 cut(s) 170, 412, 422
MluCI AATT 2 cut(s) 663, 727
MlyI GAGTC 3 cut(s) 314, 422, 481
MmeI TCCRAC 1 cut(s) 399
MseI TTAA 2 cut(s) 662, 699
MspI CCGG 1 cut(s) 280
MspR9I CCNGG 3 cut(s) 281, 624, 669
MvaI CCWGG 2 cut(s) 624, 669
MwoI GCNNNNNNNGC 5 cut(s) 110, 173, 285, 369, 526
NciI CCSGG 1 cut(s) 281
NdeII GATC 1 cut(s) 126
NlaIII CATG 6 cut(s) 58, 238, 310, 319, 487, 604
NlaIV GGNNCC 1 cut(s) 691
NmuCI GTSAC 1 cut(s) 302
NspI RCATGY 1 cut(s) 487
PciSI GCTCTTC 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 84
Pfl23II CGTACG 1 cut(s) 88
PfoI TCCNGGA 1 cut(s) 622
PkrI GCNGC 4 cut(s) 64, 82, 85, 106
PleI GAGTC 3 cut(s) 314, 421, 480
PpsI GAGTC 3 cut(s) 314, 421, 480
PsiI TTATAA 1 cut(s) 614
Psp6I CCWGG 2 cut(s) 622, 667
PspGI CCWGG 2 cut(s) 622, 667
PspLI CGTACG 1 cut(s) 88
PspN4I GGNNCC 1 cut(s) 691
PspPI GGNCC 1 cut(s) 690
RsaI GTAC 2 cut(s) 90, 383
RsaNI GTAC 2 cut(s) 89, 382
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 2 cut(s) 662, 699
SatI GCNGC 4 cut(s) 63, 81, 84, 105
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 1 cut(s) 690
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 3 cut(s) 314, 422, 481
ScrFI CCNGG 3 cut(s) 281, 624, 669
SetI ASST 9 cut(s) 32, 121, 160, 192, 230, 333, 583, 681, 717
SfaNI GCATC 1 cut(s) 472
SfcI CTRYAG 1 cut(s) 36
SinI GGWCC 1 cut(s) 690
Sse9I AATT 2 cut(s) 663, 727
SsiI CCGC 4 cut(s) 6, 62, 83, 105
SspI AATATT 1 cut(s) 299
SspMI CTAG 1 cut(s) 468
StyD4I CCNGG 3 cut(s) 279, 622, 667
StyI CCWWGG 1 cut(s) 467
TaiI ACGT 2 cut(s) 160, 192
TaqI TCGA 1 cut(s) 216
TasI AATT 2 cut(s) 663, 727
TatI WGTACW 1 cut(s) 381
TauI GCSGC 3 cut(s) 65, 86, 107
Tru1I TTAA 2 cut(s) 662, 699
Tru9I TTAA 2 cut(s) 662, 699
TscAI CASTG 2 cut(s) 74, 115
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 3 cut(s) 323, 386, 578
TspRI CASTG 2 cut(s) 74, 115
VpaK11BI GGWCC 1 cut(s) 690
XceI RCATGY 1 cut(s) 487
XcmI CCANNNNNNNNNTGG 1 cut(s) 382
XmaJI CCTAGG 1 cut(s) 467
XspI CTAG 1 cut(s) 468
ZrmI AGTACT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.