MD03G1132600.v1.1

Protein RETICULATA-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010785
Physical Location & Seq
Forward (+)
13181180 .. 13185703
4524 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1132600.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGTCAGTTTGTTCGCCACCATCGCATGCCCACCTCCCAAACGCCGTCGTTTCCAGCTCCGCCTCCGAAGGCAGAGTTCCTCTGCAATGTCTCCAAAACCCTAAATCCCATTCCGAACTCACAAACTCGTTAAAGCTGGTGGCTTCGCACCATTTTCCGTCTCGGAACCGCCGTTTCCATCTCAATTGCGCGGGATCAGAGGCTACCTCCGTCGCGGGAAAAAATGGTGATTTCACGGAGGCGAAAGCGATGGTTTTTGACCCGAAAATCGACGGTTCGGGCGGAGGAAATGACGGTGGTGATGGCGGCGATTCATTTGGTGGCGGAGGAAGAGGAGAAGGAGACGGCGGGGGTAAAGAGGATGGTGGAGAGGAAGAGGAGTTTGGGCCATTGCTGAAGTTTGATGAGGTGATGAAGGAGAGCGAAGCTCGGGGTGTGAAGCTCCCTCTAGACATGGTCGAGGCTGCAAAGACTACCGGGCTCCGGGAAGTGTTTCTTCTCCGCTACTTGGATTTGCAGGGGTCAGTTTGGCCATTGGGTTTTTTGATGAAGTACTGCTCAATGCTACGTAATCGGATGATGGCTGATCCTTCCTTTCTTTTCAAAGTTGGGACAGAGATAGTCATAGATTCTTGTTGTGCAACATTTGCAGAAGTTCAGAAAAGAGGAAAGGATTTCTGGGCTGAGTTTGAGTTGTATGCTGCTGATCTTTTGGTTGGAATAGTGGTTGACATTGCTTTGGTTGGCATGTTGGCACCCTATGCTCGGATTGGAAAGCCGTCAGTATCACAGGGTTTATTAGGAAGCGTGCAGAAGGCTTGTGCCGCTCTTCCTAGCAGCGTCTTTGAAGCCGAAAGGCCAGGATGTAAATTCACAGTTAAGCAGAGAACAGCCACATACTTCTACAAGGGTGTGTTGTACGGATCAGTTGGGTTTGTATGTGGTCTTATTGGACAAGGAATTGCAAATATGATCATGAATGCCAAAAGGAGCGTAAAAAAGTCAGAAGGGGACATACCCGTGCCTCCACTTGTGAAAAGTGCTGCTCTTTGGGGTGTGTTCCTTGCTGTCTCGTCCAACACTCGTTATCAGATCGTAAATGGTCTGGAAGGGCTGGTTGAAGCATCACCTTTGGCAAAGCGCGTTCCGCCTGTTGCAATGGCATTCACTATAGGTGTACGATTCGCCAACAACATATACGGTGGTATGCAATTTGTAGACTGGGCTAAATTGAGTGGAGTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RETICULATA-like

Protein Analysis

416

Amino Acids

44.24

Weight (kDa)

7.53

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RETICULATA-like PF11891 191 - 369 6.9e-66 Protein RETICULATA-related
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22790
fragaria_vesca FvH4_3g30080 FvH4_3g30080 FvH4_3g30080
malus_domestica MD03G1132600.v1.1 MD11G1154900.v1.1
prunus_persica Prupe.6G117100_v2.0.a1
pyrus_communis pycom11g12630 pycom11g12640
rosa_chinensis RchiOBHm_Chr5g0055771
rosa_laevigata RLG00000035009
rosa_multiflora Rmu_sc0007355.1_g000014 Rmu_sc0037521.1_g000001
rosa_roxburghii Rroxscaffold_1G00024560
rosa_rugosa Rorug05G0294800
rosa_samantha Rh5AG365700 Rh5BG376200 Rh5CG399300 Rh5DG389300
rosa_wichuraiana Rw5G034300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 754
AccBSI CCGCTC 1 cut(s) 827
AccI GTMKAC 1 cut(s) 1218
AccII CGCG 3 cut(s) 191, 215, 1143
AclWI GGATC 3 cut(s) 202, 581, 931
AcoI YGGCCR 1 cut(s) 530
AcsI RAATTY 1 cut(s) 869
AcuI CTGAAG 1 cut(s) 416
AfaI GTAC 3 cut(s) 554, 920, 1179
AfiI CCNNNNNNNGG 3 cut(s) 483, 508, 765
AgsI TTSAA 3 cut(s) 604, 848, 1121
AjnI CCWGG 1 cut(s) 859
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
AluBI AGCT 4 cut(s) 57, 136, 428, 442
AluI AGCT 4 cut(s) 57, 136, 428, 442
Alw26I GTCTC 4 cut(s) 95, 165, 336, 1075
AlwI GGATC 3 cut(s) 202, 581, 931
Ama87I CYCGRG 1 cut(s) 429
AoxI GGCC 3 cut(s) 386, 530, 857
ApeKI GCWGC 4 cut(s) 464, 701, 837, 1043
ApoI RAATTY 1 cut(s) 869
Asp700I GAANNNNTTC 1 cut(s) 492
AspLEI GCGC 2 cut(s) 191, 1143
AspS9I GGNCC 1 cut(s) 386
AsuC2I CCSGG 2 cut(s) 478, 485
AsuHPI GGTGA 4 cut(s) 239, 311, 421, 1119
AvaI CYCGRG 1 cut(s) 429
BalI TGGCCA 1 cut(s) 532
BanI GGYRCC 1 cut(s) 754
BanII GRGCYC 1 cut(s) 483
BarI GAAGNNNNNNTAC 2 cut(s) 999, 1031
BbvI GCAGC 4 cut(s) 451, 688, 849, 1030
BccI CCATC 6 cut(s) 28, 186, 244, 296, 356, 574
BceAI ACGGC 4 cut(s) 29, 156, 361, 763
BciT130I CCWGG 1 cut(s) 861
BclI TGATCA 1 cut(s) 972
BcnI CCSGG 2 cut(s) 478, 485
BcoDI GTCTC 4 cut(s) 95, 165, 336, 1075
BfaI CTAG 2 cut(s) 449, 834
BfmI CTRYAG 1 cut(s) 1170
BisI GCNGC 6 cut(s) 307, 465, 702, 825, 838, 1044
BlsI GCNGC 6 cut(s) 308, 466, 703, 826, 839, 1045
BmcAI AGTACT 1 cut(s) 554
Bme1390I CCNGG 3 cut(s) 478, 485, 861
BmeT110I CYCGRG 1 cut(s) 429
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 3 cut(s) 167, 482, 756
BmrFI CCNGG 3 cut(s) 478, 485, 861
BmrI ACTGGG 1 cut(s) 1231
BmsI GCATC 1 cut(s) 1133
BmuI ACTGGG 1 cut(s) 1231
BplI GAGNNNNNCTC 4 cut(s) 191, 223, 412, 444
BpuMI CCSGG 2 cut(s) 478, 485
BsaAI YACGTR 1 cut(s) 569
Bsc4I CCNNNNNNNGG 3 cut(s) 483, 508, 765
Bse1I ACTGG 1 cut(s) 1226
Bse3DI GCAATG 4 cut(s) 92, 389, 732, 1164
BseBI CCWGG 1 cut(s) 861
BseGI GGATG 3 cut(s) 367, 582, 869
BseLI CCNNNNNNNGG 3 cut(s) 483, 508, 765
BseMI GCAATG 4 cut(s) 92, 389, 732, 1164
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 1 cut(s) 1226
BseRI GAGGAG 2 cut(s) 348, 392
BseXI GCAGC 4 cut(s) 451, 688, 849, 1030
BsgI GTGCAG 1 cut(s) 830
Bsh1236I CGCG 3 cut(s) 191, 215, 1143
BshFI GGCC 3 cut(s) 388, 532, 859
BshNI GGYRCC 1 cut(s) 754
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 2 cut(s) 477, 484
BslFI GGGAC 2 cut(s) 625, 1025
BslI CCNNNNNNNGG 3 cut(s) 483, 508, 765
BsmAI GTCTC 4 cut(s) 95, 165, 336, 1075
BsmBI CGTCTC 2 cut(s) 165, 336
BsmFI GGGAC 2 cut(s) 625, 1025
BsmI GAATGC 2 cut(s) 985, 1163
BsnI GGCC 3 cut(s) 388, 532, 859
BsoBI CYCGRG 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 483
Bsp143I GATC 6 cut(s) 194, 586, 706, 923, 972, 1092
BspANI GGCC 3 cut(s) 388, 532, 859
BspCNI CTCAG 1 cut(s) 676
BspFNI CGCG 3 cut(s) 191, 215, 1143
BspHI TCATGA 1 cut(s) 975
BspLI GGNNCC 3 cut(s) 167, 482, 756
BspPI GGATC 3 cut(s) 202, 581, 931
BspQI GCTCTTC 1 cut(s) 834
BspT107I GGYRCC 1 cut(s) 754
BsrBI CCGCTC 1 cut(s) 827
BsrDI GCAATG 4 cut(s) 92, 389, 732, 1164
BsrI ACTGG 1 cut(s) 1226
BssMI GATC 6 cut(s) 194, 586, 706, 923, 972, 1092
Bst2UI CCWGG 1 cut(s) 861
Bst4CI ACNGT 4 cut(s) 275, 296, 877, 1202
Bst6I CTCTTC 3 cut(s) 325, 369, 834
BstAPI GCANNNNNTGC 2 cut(s) 647, 761
BstBAI YACGTR 1 cut(s) 569
BstC8I GCNNGC 2 cut(s) 27, 809
BstDEI CTNAG 1 cut(s) 684
BstF5I GGATG 3 cut(s) 367, 582, 869
BstFNI CGCG 3 cut(s) 191, 215, 1143
BstHHI GCGC 2 cut(s) 191, 1143
BstKTI GATC 6 cut(s) 197, 589, 709, 926, 975, 1095
BstMAI GTCTC 4 cut(s) 95, 165, 336, 1075
BstMBI GATC 6 cut(s) 194, 586, 706, 923, 972, 1092
BstMWI GCNNNNNNNGC 5 cut(s) 22, 647, 761, 824, 1147
BstNI CCWGG 1 cut(s) 861
BstNSI RCATGY 2 cut(s) 29, 751
BstSCI CCNGG 3 cut(s) 476, 483, 859
BstSFI CTRYAG 1 cut(s) 1170
BstSNI TACGTA 1 cut(s) 569
BstUI CGCG 3 cut(s) 191, 215, 1143
BstV1I GCAGC 4 cut(s) 451, 688, 849, 1030
BsuRI GGCC 3 cut(s) 388, 532, 859
BtgZI GCGATG 2 cut(s) 6, 263
BtsCI GGATG 3 cut(s) 367, 582, 869
Cac8I GCNNGC 2 cut(s) 27, 809
CciI TCATGA 1 cut(s) 975
CfoI GCGC 2 cut(s) 191, 1143
Cfr13I GGNCC 1 cut(s) 386
CseI GACGC 1 cut(s) 829
Csp6I GTAC 3 cut(s) 553, 919, 1178
CviAII CATG 4 cut(s) 26, 454, 748, 976
CviQI GTAC 3 cut(s) 553, 919, 1178
DdeI CTNAG 1 cut(s) 684
DpnI GATC 6 cut(s) 196, 588, 708, 925, 974, 1094
DpnII GATC 6 cut(s) 194, 586, 706, 923, 972, 1092
EaeI YGGCCR 1 cut(s) 530
Eam1104I CTCTTC 3 cut(s) 325, 369, 834
EarI CTCTTC 3 cut(s) 325, 369, 834
EciI GGCGGA 4 cut(s) 49, 297, 339, 1137
Eco105I TACGTA 1 cut(s) 569
Eco24I GRGCYC 1 cut(s) 483
Eco57I CTGAAG 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 429
EcoRII CCWGG 1 cut(s) 859
EcoT38I GRGCYC 1 cut(s) 483
Esp3I CGTCTC 2 cut(s) 165, 336
FaeI CATG 4 cut(s) 29, 457, 751, 979
FalI AAGNNNNNCTT 2 cut(s) 480, 512
FaqI GGGAC 2 cut(s) 625, 1025
FatI CATG 4 cut(s) 25, 453, 747, 975
FauI CCCGC 3 cut(s) 184, 208, 341
FbaI TGATCA 1 cut(s) 972
FblI GTMKAC 1 cut(s) 1218
Fnu4HI GCNGC 6 cut(s) 307, 465, 702, 825, 838, 1044
FokI GGATG 3 cut(s) 374, 589, 876
FriOI GRGCYC 1 cut(s) 483
Fsp4HI GCNGC 6 cut(s) 307, 465, 702, 825, 838, 1044
FspBI CTAG 2 cut(s) 449, 834
GlaI GCGC 2 cut(s) 190, 1142
GluI GCNGC 6 cut(s) 307, 465, 702, 825, 838, 1044
HaeIII GGCC 3 cut(s) 388, 532, 859
HapII CCGG 2 cut(s) 477, 484
HgaI GACGC 1 cut(s) 829
HhaI GCGC 2 cut(s) 191, 1143
Hin1II CATG 4 cut(s) 29, 457, 751, 979
Hin6I GCGC 2 cut(s) 189, 1141
HinP1I GCGC 2 cut(s) 189, 1141
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HinfI GANTC 3 cut(s) 311, 629, 1182
HpaII CCGG 2 cut(s) 477, 484
HphI GGTGA 4 cut(s) 239, 311, 421, 1119
Hpy166II GTNNAC 3 cut(s) 730, 1178, 1219
Hpy188I TCNGA 9 cut(s) 67, 115, 165, 199, 576, 660, 768, 1006, 1092
Hpy188III TCNNGA 3 cut(s) 449, 976, 1106
Hpy8I GTNNAC 3 cut(s) 730, 1178, 1219
Hpy99I CGWCG 3 cut(s) 50, 215, 275
HpyAV CCTTC 7 cut(s) 62, 332, 409, 600, 808, 1001, 1103
HpyCH4III ACNGT 4 cut(s) 275, 296, 877, 1202
HpyCH4IV ACGT 1 cut(s) 568
HpyF10VI GCNNNNNNNGC 5 cut(s) 22, 647, 761, 824, 1147
HpyF3I CTNAG 1 cut(s) 684
HpySE526I ACGT 1 cut(s) 568
Hsp92II CATG 4 cut(s) 29, 457, 751, 979
HspAI GCGC 2 cut(s) 189, 1141
Ksp22I TGATCA 1 cut(s) 972
Kzo9I GATC 6 cut(s) 194, 586, 706, 923, 972, 1092
LguI GCTCTTC 1 cut(s) 834
LmnI GCTCC 4 cut(s) 62, 447, 486, 990
Lsp1109I GCAGC 4 cut(s) 451, 688, 849, 1030
LweI GCATC 1 cut(s) 1133
MaeI CTAG 2 cut(s) 449, 834
MaeII ACGT 1 cut(s) 568
MalI GATC 6 cut(s) 196, 588, 708, 925, 974, 1094
MbiI CCGCTC 1 cut(s) 827
MboI GATC 6 cut(s) 194, 586, 706, 923, 972, 1092
MboII GAAGA 4 cut(s) 342, 386, 488, 821
MfeI CAATTG 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 483
MlsI TGGCCA 1 cut(s) 532
MluCI AATT 5 cut(s) 184, 869, 960, 1211, 1229
MluNI TGGCCA 1 cut(s) 532
MmeI TCCRAC 2 cut(s) 697, 1101
Mox20I TGGCCA 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 492
MscI TGGCCA 1 cut(s) 532
MseI TTAA 2 cut(s) 131, 879
MslI CAYNNNNRTG 1 cut(s) 1019
Msp20I TGGCCA 1 cut(s) 532
MspI CCGG 2 cut(s) 477, 484
MspR9I CCNGG 3 cut(s) 478, 485, 861
MunI CAATTG 1 cut(s) 184
Mva1269I GAATGC 2 cut(s) 985, 1163
MvaI CCWGG 1 cut(s) 861
MvnI CGCG 3 cut(s) 191, 215, 1143
MwoI GCNNNNNNNGC 5 cut(s) 22, 647, 761, 824, 1147
NciI CCSGG 2 cut(s) 478, 485
NdeII GATC 6 cut(s) 194, 586, 706, 923, 972, 1092
NlaIII CATG 4 cut(s) 29, 457, 751, 979
NlaIV GGNNCC 3 cut(s) 167, 482, 756
NspI RCATGY 2 cut(s) 29, 751
PaeI GCATGC 1 cut(s) 29
PagI TCATGA 1 cut(s) 975
PciSI GCTCTTC 1 cut(s) 834
PcsI WCGNNNNNNNCGW 1 cut(s) 169
PctI GAATGC 2 cut(s) 985, 1163
PdmI GAANNNNTTC 1 cut(s) 492
PfeI GAWTC 3 cut(s) 311, 629, 1182
PflFI GACNNNGTC 1 cut(s) 455
PfoI TCCNGGA 1 cut(s) 483
PkrI GCNGC 6 cut(s) 308, 466, 703, 826, 839, 1045
Ppu21I YACGTR 1 cut(s) 569
Psp6I CCWGG 1 cut(s) 859
PspGI CCWGG 1 cut(s) 859
PspN4I GGNNCC 3 cut(s) 167, 482, 756
PspPI GGNCC 1 cut(s) 386
PsyI GACNNNGTC 1 cut(s) 455
RsaI GTAC 3 cut(s) 554, 920, 1179
RsaNI GTAC 3 cut(s) 553, 919, 1178
RseI CAYNNNNRTG 1 cut(s) 1019
SapI GCTCTTC 1 cut(s) 834
SaqAI TTAA 2 cut(s) 131, 879
SatI GCNGC 6 cut(s) 307, 465, 702, 825, 838, 1044
Sau3AI GATC 6 cut(s) 194, 586, 706, 923, 972, 1092
Sau96I GGNCC 1 cut(s) 386
ScaI AGTACT 1 cut(s) 554
ScrFI CCNGG 3 cut(s) 478, 485, 861
SduI GDGCHC 1 cut(s) 483
SfaNI GCATC 1 cut(s) 1133
SfcI CTRYAG 1 cut(s) 1170
SmiMI CAYNNNNRTG 1 cut(s) 1019
SnaBI TACGTA 1 cut(s) 569
SphI GCATGC 1 cut(s) 29
Sse9I AATT 5 cut(s) 184, 869, 960, 1211, 1229
SspMI CTAG 2 cut(s) 449, 834
StyD4I CCNGG 3 cut(s) 476, 483, 859
TaaI ACNGT 4 cut(s) 275, 296, 877, 1202
TaiI ACGT 1 cut(s) 571
TaqI TCGA 2 cut(s) 270, 459
TasI AATT 5 cut(s) 184, 869, 960, 1211, 1229
TatI WGTACW 1 cut(s) 552
TauI GCSGC 2 cut(s) 309, 827
TfiI GAWTC 3 cut(s) 311, 629, 1182
Tru1I TTAA 2 cut(s) 131, 879
Tru9I TTAA 2 cut(s) 131, 879
TseI GCWGC 4 cut(s) 464, 701, 837, 1043
TspDTI ATGAA 4 cut(s) 303, 428, 563, 992
TspGWI ACGGA 4 cut(s) 147, 199, 251, 936
Tth111I GACNNNGTC 1 cut(s) 455
XapI RAATTY 1 cut(s) 869
XbaI TCTAGA 1 cut(s) 448
XceI RCATGY 2 cut(s) 29, 751
XmiI GTMKAC 1 cut(s) 1218
XmnI GAANNNNTTC 1 cut(s) 492
XspI CTAG 2 cut(s) 449, 834
ZrmI AGTACT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.