MD03G1163200.v1.1

Tryptophan/tyrosine permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004761
Physical Location & Seq
Forward (+)
20310444 .. 20314543
4100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1163200.v1.1.491

Sequence Viewer

Length: 855 bp
ATGGCAGCAGCTTTCTGTCGTTCTGTTTCTTGGGTTGTTAGTCCTCCGTGGTGGTGGTCGTCGCCGTCGTCAAGTTTGTCGTTGCATTATTCTGGTCATCGTCCACTCCCCCTTGCACATAGTTATTCGACATCATATACAAAGACGAGGAGGAGGAGCAAGAGGAGGAGCGATTACTCAAGTCGAGGTGAAAGAAGAAATGTAGTTATCAAATGCCTCTCAAAGGAGAGTGAGACGGAGGCGGAATTTGAGTTGCAGCGGCGTCTCTTTTCCAACCTCAACCAAACCACATTGAAGAGAGAACCTGGAAGTCTAGCCAGCGCTATTTTGCTAGTCGCAGGGACAACGGTGGGTGCCGGCATCCTTGCAATTCCTGCAGTCACACTAGAATCTGGATTTCTGGCTTCTGCGCTTACATGCATCCTTTGTTGGTTATACATGGTTGCAACAGGACTGCTAATAGCCCAAGTCAATGTAAACACTATGTGCGAATTGGGTTCCGGCAGTGTATCACTGGTATCAATGGCTATGAGGACCATGGGGAGTGTGGGAGTTCAAATTGTTTGCAAATTACTGATCTCAACGTGCATACTTTGGGTTTTTGTTTTGTTTGGTTTTGTCCTGAATACTCTGGTTTCTGTAACTGTAGTCCAAGCTGGAGGGAGAAGATCAACTTGGACTGATAGATCATTGGACATAGACATACTCGATTATTTCAACATAGAAATGACTTTCATTTTCTTGTTTTCATTTAATTTTTTGTTCCTTATGCTTTTTATGACTCTCTGGGACAACCTCGAAGACTTGGACCAATGGATAAGCCCATGCCCATGCCAAACTAATTGCACCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trp_Tyr_perm

Protein Analysis

285

Amino Acids

31.71

Weight (kDa)

8.55

Isoelectric Point (pI)

55.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trp_Tyr_perm PF03222 104 - 192 2.3e-10 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 353
AciI CCGC 2 cut(s) 242, 259
AcsI RAATTY 1 cut(s) 245
AcyI GRCGYC 1 cut(s) 262
AdeI CACNNNGTG 1 cut(s) 486
AfeI AGCGCT 1 cut(s) 322
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 3 cut(s) 295, 557, 718
AjnI CCWGG 1 cut(s) 304
AjuI GAANNNNNNNTTGG 2 cut(s) 658, 690
AluBI AGCT 2 cut(s) 11, 656
AluI AGCT 2 cut(s) 11, 656
Alw26I GTCTC 2 cut(s) 227, 269
Aor51HI AGCGCT 1 cut(s) 322
ApeKI GCWGC 3 cut(s) 5, 8, 256
ApoI RAATTY 1 cut(s) 245
AspLEI GCGC 2 cut(s) 323, 412
AspS9I GGNCC 2 cut(s) 534, 808
AsuHPI GGTGA 1 cut(s) 200
AvaII GGWCC 2 cut(s) 534, 808
BanI GGYRCC 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 807
BbvI GCAGC 3 cut(s) 17, 20, 268
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 1 cut(s) 306
BcoDI GTCTC 2 cut(s) 227, 269
BfaI CTAG 3 cut(s) 314, 332, 386
BfmI CTRYAG 2 cut(s) 375, 645
BfoI RGCGCY 1 cut(s) 324
BisI GCNGC 4 cut(s) 6, 9, 257, 260
BlsI GCNGC 4 cut(s) 7, 10, 258, 261
Bme1390I CCNGG 1 cut(s) 306
Bme18I GGWCC 2 cut(s) 534, 808
BmgT120I GGNCC 2 cut(s) 534, 808
BmiI GGNNCC 2 cut(s) 355, 499
BmrFI CCNGG 1 cut(s) 306
BmsI GCATC 2 cut(s) 369, 429
BpiI GAAGAC 1 cut(s) 807
BpmI CTGGAG 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 163
BsaHI GRCGYC 1 cut(s) 262
BsaJI CCNNGG 2 cut(s) 47, 537
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 2 cut(s) 47, 537
BseGI GGATG 2 cut(s) 360, 420
BseLI CCNNNNNNNGG 1 cut(s) 223
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 5 cut(s) 163, 166, 169, 178, 181
BseXI GCAGC 3 cut(s) 17, 20, 268
BshNI GGYRCC 1 cut(s) 353
BsiSI CCGG 2 cut(s) 357, 501
BslFI GGGAC 2 cut(s) 355, 803
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 2 cut(s) 227, 269
BsmBI CGTCTC 2 cut(s) 227, 269
BsmFI GGGAC 2 cut(s) 355, 803
Bsp143I GATC 3 cut(s) 576, 668, 686
Bsp19I CCATGG 1 cut(s) 537
BspACI CCGC 2 cut(s) 242, 259
BspLI GGNNCC 2 cut(s) 355, 499
BspMAI CTGCAG 1 cut(s) 379
BspT107I GGYRCC 1 cut(s) 353
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 1 cut(s) 519
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 2 cut(s) 47, 537
BssMI GATC 3 cut(s) 576, 668, 686
BssNI GRCGYC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 2 cut(s) 349, 646
Bst6I CTCTTC 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 262
BstAPI GCANNNNNTGC 1 cut(s) 374
BstC8I GCNNGC 2 cut(s) 319, 358
BstDSI CCRYGG 2 cut(s) 47, 537
BstENI CCTNNNNNAGG 1 cut(s) 221
BstF5I GGATG 2 cut(s) 360, 420
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 2 cut(s) 323, 412
BstKTI GATC 3 cut(s) 579, 671, 689
BstMAI GTCTC 2 cut(s) 227, 269
BstMBI GATC 3 cut(s) 576, 668, 686
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 1 cut(s) 420
BstSCI CCNGG 1 cut(s) 304
BstSFI CTRYAG 2 cut(s) 375, 645
BstV1I GCAGC 3 cut(s) 17, 20, 268
BstV2I GAAGAC 1 cut(s) 807
BtgI CCRYGG 2 cut(s) 47, 537
BtsCI GGATG 2 cut(s) 360, 420
BtsI GCAGTG 1 cut(s) 511
BtsIMutI CAGTG 2 cut(s) 511, 512
Cac8I GCNNGC 2 cut(s) 319, 358
CfoI GCGC 2 cut(s) 323, 412
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 2 cut(s) 534, 808
CseI GACGC 1 cut(s) 251
CviAII CATG 5 cut(s) 417, 439, 538, 825, 831
CviJI RGCY 7 cut(s) 11, 317, 404, 464, 527, 656, 822
CviKI_1 RGCY 7 cut(s) 11, 317, 404, 464, 527, 656, 822
DpnI GATC 3 cut(s) 578, 670, 688
DpnII GATC 3 cut(s) 576, 668, 686
DraIII CACNNNGTG 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
EciI GGCGGA 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 537
Eco47I GGWCC 2 cut(s) 534, 808
Eco47III AGCGCT 1 cut(s) 322
EcoNI CCTNNNNNAGG 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 537
EcoT22I ATGCAT 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 537
Esp3I CGTCTC 2 cut(s) 227, 269
FaeI CATG 5 cut(s) 420, 442, 541, 828, 834
FalI AAGNNNNNCTT 2 cut(s) 658, 690
FaqI GGGAC 2 cut(s) 355, 803
FatI CATG 5 cut(s) 416, 438, 537, 824, 830
Fnu4HI GCNGC 4 cut(s) 6, 9, 257, 260
FokI GGATG 2 cut(s) 347, 407
Fsp4HI GCNGC 4 cut(s) 6, 9, 257, 260
FspBI CTAG 3 cut(s) 314, 332, 386
GlaI GCGC 2 cut(s) 322, 411
GluI GCNGC 4 cut(s) 6, 9, 257, 260
GsuI CTGGAG 1 cut(s) 678
HaeII RGCGCY 1 cut(s) 324
HapII CCGG 2 cut(s) 357, 501
HgaI GACGC 1 cut(s) 251
HhaI GCGC 2 cut(s) 323, 412
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 5 cut(s) 420, 442, 541, 828, 834
Hin6I GCGC 2 cut(s) 321, 410
HinP1I GCGC 2 cut(s) 321, 410
HinfI GANTC 2 cut(s) 389, 781
HpaII CCGG 2 cut(s) 357, 501
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 104, 478
Hpy188III TCNNGA 2 cut(s) 393, 622
Hpy8I GTNNAC 2 cut(s) 104, 478
Hpy99I CGWCG 2 cut(s) 64, 70
HpyCH4III ACNGT 2 cut(s) 349, 646
HpyCH4IV ACGT 1 cut(s) 584
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpySE526I ACGT 1 cut(s) 584
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 5 cut(s) 420, 442, 541, 828, 834
HspAI GCGC 2 cut(s) 321, 410
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Kzo9I GATC 3 cut(s) 576, 668, 686
LmnI GCTCC 2 cut(s) 156, 168
Lsp1109I GCAGC 3 cut(s) 17, 20, 268
LweI GCATC 2 cut(s) 369, 429
MaeI CTAG 3 cut(s) 314, 332, 386
MaeII ACGT 1 cut(s) 584
MaeIII GTNAC 2 cut(s) 379, 640
MalI GATC 3 cut(s) 578, 670, 688
MboI GATC 3 cut(s) 576, 668, 686
MboII GAAGA 4 cut(s) 207, 307, 678, 812
MluCI AATT 7 cut(s) 245, 369, 491, 558, 569, 754, 841
MlyI GAGTC 1 cut(s) 775
MmeI TCCRAC 1 cut(s) 297
Mph1103I ATGCAT 1 cut(s) 422
MroNI GCCGGC 1 cut(s) 356
MseI TTAA 1 cut(s) 753
MslI CAYNNNNRTG 2 cut(s) 725, 829
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 2 cut(s) 357, 501
MspR9I CCNGG 1 cut(s) 306
MvaI CCWGG 1 cut(s) 306
MwoI GCNNNNNNNGC 1 cut(s) 374
NaeI GCCGGC 1 cut(s) 358
NcoI CCATGG 1 cut(s) 537
NdeII GATC 3 cut(s) 576, 668, 686
NgoMIV GCCGGC 1 cut(s) 356
NlaIII CATG 5 cut(s) 420, 442, 541, 828, 834
NlaIV GGNNCC 2 cut(s) 355, 499
NmuCI GTSAC 1 cut(s) 379
NsiI ATGCAT 1 cut(s) 422
NspI RCATGY 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 1 cut(s) 389
PkrI GCNGC 4 cut(s) 7, 10, 258, 261
PleI GAGTC 1 cut(s) 775
PpsI GAGTC 1 cut(s) 775
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 2 cut(s) 355, 499
PspPI GGNCC 2 cut(s) 534, 808
PstI CTGCAG 1 cut(s) 379
RseI CAYNNNNRTG 2 cut(s) 725, 829
SaqAI TTAA 1 cut(s) 753
SatI GCNGC 4 cut(s) 6, 9, 257, 260
Sau3AI GATC 3 cut(s) 576, 668, 686
Sau96I GGNCC 2 cut(s) 534, 808
SchI GAGTC 1 cut(s) 775
ScrFI CCNGG 1 cut(s) 306
SetI ASST 7 cut(s) 13, 190, 279, 307, 587, 658, 798
SfaNI GCATC 2 cut(s) 369, 429
SfcI CTRYAG 2 cut(s) 375, 645
SinI GGWCC 2 cut(s) 534, 808
SmiMI CAYNNNNRTG 2 cut(s) 725, 829
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
Sse9I AATT 7 cut(s) 245, 369, 491, 558, 569, 754, 841
SsiI CCGC 2 cut(s) 242, 259
SspMI CTAG 3 cut(s) 314, 332, 386
StyD4I CCNGG 1 cut(s) 304
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 2 cut(s) 349, 646
TaiI ACGT 1 cut(s) 587
TaqI TCGA 4 cut(s) 128, 184, 708, 798
TasI AATT 7 cut(s) 245, 369, 491, 558, 569, 754, 841
TauI GCSGC 1 cut(s) 262
TfiI GAWTC 1 cut(s) 389
Tru1I TTAA 1 cut(s) 753
Tru9I TTAA 1 cut(s) 753
TscAI CASTG 2 cut(s) 511, 519
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 3 cut(s) 5, 8, 256
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 2 cut(s) 724, 738
TspGWI ACGGA 2 cut(s) 36, 251
TspRI CASTG 2 cut(s) 511, 519
VpaK11BI GGWCC 2 cut(s) 534, 808
XagI CCTNNNNNAGG 1 cut(s) 221
XapI RAATTY 1 cut(s) 245
XceI RCATGY 1 cut(s) 420
XcmI CCANNNNNNNNNTGG 1 cut(s) 544
XspI CTAG 3 cut(s) 314, 332, 386
Zsp2I ATGCAT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.