Rroxscaffold_1G00031040

Tryptophan/tyrosine permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004761
Physical Location & Seq
Forward (+)
43348743 .. 43359576
10834 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00031040.1

Sequence Viewer

Length: 1566 bp
ATGCACAATTCCAAACTCCTTGCCGATACCATGGCGAAAGCGGGACACCATGCCTCGGCTCAAAGATGGTCTCGGTCTCTTCCCGGGTTTGCCACTCAAGCTTTGCTTCATGATACCTTGAATTTTGAAAAGGCAATGGCATTTCTGGCTTCTCCTCCTCCTCCTCCTCCTCCTTGTTGTTGCGTTGGTTCAAGATTGGTGCAAAAGCAAATTCACGCTCTTCCACTCGCACTCCCACTCCCACTTGATCATAAACCACTTTATCTCCCAAATCCAAATCCAAGAAAATTTGCGAAATGCCTGTCGCAGAAGAAACAATCCGAAGAGGCTGAGTTGGAAAGGCTGTTTTCCAACCTCAACCAAGCCACATTGAAGAGAGAACCCCCAAGTCTATCCAGTGCTGTTTTCCTAGTTGCAGGGACTACGGTGGGTGCTGGTATCCTCGCAATACCTGCAGTGACCCAAGAATCTGGATTTCTGGCCTCTGCAATAACCTGCATTTTTTGTTGGGCATACATGGTTGTAACGGGTCTGCTAATAGCCGAAGTTAATGTAAATACTATGTCTCAACTCGGTTCTGGCAGTGTATCACTGGTATCCATGGCTATGAGGACTGTTGGTAATGTGGGAGTTCAAATTGTTTGTTGGACGTACATTTTTATACATTACGCACTTCTTGTTGCCTATGTTGCTCGTTCTTCAGAAATTTTGACAAATTTTGTTGGCATTCCCTTATGGGAAAGTGCAACATTGTTTTCATTGGTTTTCGGAGGCATTTGCTACTTTGGAAGTCCGCAGTTCATTGGTGCGGTTAATGGGGTTCTGGTAATTGGAATCATCATGTCTTTTGCTGCTCTTGTGGCAGTTGCAAGTGAATACCTACAATTGAGTGCACTTTTGGAAGCCAACTTCGAAGCTGTTCCCATGAGCATACCGATAATTGTTTTGTCATTTGTATACCAGAATGTAGTGCCTGTTCTTTGTACAGATCTTGAAGGAGACCTATCAAAAGTAAGGACTTCAATTGTTCTAGGCACAAGTATACCACTTATTTTATTTCTAGTCTGGAATGCTGTGATTCTAGGAACCATCAGAAATGTTGAAATGGGCTCTGGTAAAATCATGGATCCATTACAACTATTGCAATCAACAAATGCTGCTGTTGGACCTATAGTTGAGGTGTTCTCAATTTTTGCTATCGCGACGTCATATATTGGATTTGTTTTGGGTCTCTGTGATTTCCTTTCTGATTTGCTGAAACTCCCGGCTGGTCAGAGCAGGCCTGTGCTGCCGTACCTTTTAACGTTGATTCCACCAGTTGTGCTGTCATTGCTAGACCCAGATATATTCTTTAAAGCCTTGGACTTTGCTGGAACTTATGGAGTGTTGGTGCTGTTCGGAATTGTTCCAGCTGCAATGTCTTGGTCCGATAGGTACTCAAGTTCATCCACAGCTGTGAAATTGCCGCAGTTGGTCCCAGGAGGAAGGCTTACTCTTTCGCTTGTGGTAGGAGGTGCTGGATATGTCGTTTTCTCAGAAATTATTGACAAGTTGAGCAACCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trp_Tyr_perm

Protein Analysis

521

Amino Acids

55.7

Weight (kDa)

6.65

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trp_Tyr_perm PF03222 129 - 505 4.9e-73 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1208
Acc36I ACCTGC 2 cut(s) 460, 503
AccI GTMKAC 2 cut(s) 957, 1042
AccII CGCG 1 cut(s) 1202
AciI CCGC 4 cut(s) 41, 794, 809, 1466
AclI AACGTT 1 cut(s) 1304
AclWI GGATC 2 cut(s) 1121, 1134
AcsI RAATTY 5 cut(s) 121, 210, 287, 705, 715
AcuI CTGAAG 1 cut(s) 684
AcyI GRCGYC 1 cut(s) 1205
AfaI GTAC 4 cut(s) 653, 985, 1295, 1436
AfiI CCNNNNNNNGG 1 cut(s) 55
AgsI TTSAA 8 cut(s) 121, 128, 192, 373, 635, 995, 1023, 1103
AjnI CCWGG 1 cut(s) 1477
AleI CACNNNNGTG 1 cut(s) 1454
AluBI AGCT 4 cut(s) 101, 917, 1412, 1454
AluI AGCT 4 cut(s) 101, 917, 1412, 1454
Alw21I GWGCWC 1 cut(s) 895
Alw26I GTCTC 5 cut(s) 75, 81, 570, 993, 1235
Alw44I GTGCAC 1 cut(s) 891
AlwI GGATC 2 cut(s) 1121, 1134
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 2 cut(s) 480, 1280
ApaLI GTGCAC 1 cut(s) 891
ApeKI GCWGC 4 cut(s) 851, 1157, 1288, 1412
ApoI RAATTY 5 cut(s) 121, 210, 287, 705, 715
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
Asp700I GAANNNNTTC 1 cut(s) 918
AspS9I GGNCC 3 cut(s) 1166, 1425, 1474
AsuC2I CCSGG 3 cut(s) 84, 85, 1265
AsuII TTCGAA 1 cut(s) 912
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 3 cut(s) 1166, 1425, 1474
BaeGI GKGCMC 1 cut(s) 895
BaeI ACNNNNGTAYC 2 cut(s) 1426, 1459
BamHI GGATCC 1 cut(s) 1126
BanII GRGCYC 1 cut(s) 1112
Bbv12I GWGCWC 1 cut(s) 895
BbvI GCAGC 4 cut(s) 838, 1144, 1275, 1399
BccI CCATC 2 cut(s) 60, 1097
BceAI ACGGC 1 cut(s) 1276
BciT130I CCWGG 1 cut(s) 1479
BciVI GTATCC 2 cut(s) 449, 607
BclI TGATCA 1 cut(s) 247
BcnI CCSGG 3 cut(s) 84, 85, 1265
BcoDI GTCTC 5 cut(s) 75, 81, 570, 993, 1235
BfaI CTAG 5 cut(s) 410, 1031, 1061, 1082, 1334
BfmI CTRYAG 2 cut(s) 453, 1170
BfuAI ACCTGC 2 cut(s) 460, 503
BfuI GTATCC 2 cut(s) 449, 607
BglII AGATCT 1 cut(s) 988
BisI GCNGC 5 cut(s) 852, 1158, 1289, 1413, 1466
BlsI GCNGC 5 cut(s) 853, 1159, 1290, 1414, 1467
Bme1390I CCNGG 4 cut(s) 84, 85, 1265, 1479
Bme18I GGWCC 3 cut(s) 1166, 1425, 1474
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 3 cut(s) 1166, 1425, 1474
BmiI GGNNCC 3 cut(s) 1087, 1128, 1476
BmrFI CCNGG 4 cut(s) 84, 85, 1265, 1479
BplI GAGNNNNNCTC 2 cut(s) 1169, 1201
Bpu14I TTCGAA 1 cut(s) 912
BpuEI CTTGAG 2 cut(s) 81, 1423
BpuMI CCSGG 3 cut(s) 84, 85, 1265
BsaHI GRCGYC 1 cut(s) 1205
BsaI GGTCTC 4 cut(s) 75, 81, 993, 1235
BsaJI CCNNGG 6 cut(s) 30, 54, 83, 600, 1359, 1477
BsaXI ACNNNNNCTCC 8 cut(s) 216, 222, 246, 249, 252, 279, 1374, 1404
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 396, 597, 1316
Bse3DI GCAATG 3 cut(s) 141, 1328, 1422
BseBI CCWGG 1 cut(s) 1479
BseDI CCNNGG 6 cut(s) 30, 54, 83, 600, 1359, 1477
BseGI GGATG 1 cut(s) 1445
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 3 cut(s) 141, 1328, 1422
BseMII CTCAG 2 cut(s) 321, 1548
BseNI ACTGG 3 cut(s) 396, 597, 1316
BseRI GAGGAG 6 cut(s) 144, 147, 150, 153, 156, 159
BseSI GKGCMC 1 cut(s) 895
BseXI GCAGC 4 cut(s) 838, 1144, 1275, 1399
Bsh1236I CGCG 1 cut(s) 1202
BshFI GGCC 2 cut(s) 482, 1282
BsiHKAI GWGCWC 1 cut(s) 895
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 2 cut(s) 84, 1265
BslFI GGGAC 3 cut(s) 57, 433, 1460
BslI CCNNNNNNNGG 1 cut(s) 55
BsmAI GTCTC 5 cut(s) 75, 81, 570, 993, 1235
BsmFI GGGAC 3 cut(s) 57, 433, 1460
BsmI GAATGC 2 cut(s) 726, 1075
BsnI GGCC 2 cut(s) 482, 1282
Bso31I GGTCTC 4 cut(s) 75, 81, 993, 1235
BsoBI CYCGRG 1 cut(s) 83
Bsp119I TTCGAA 1 cut(s) 912
Bsp1286I GDGCHC 2 cut(s) 895, 1112
Bsp1407I TGTACA 1 cut(s) 983
Bsp143I GATC 3 cut(s) 247, 988, 1126
Bsp19I CCATGG 2 cut(s) 30, 600
Bsp68I TCGCGA 1 cut(s) 1202
BspACI CCGC 4 cut(s) 41, 794, 809, 1466
BspANI GGCC 2 cut(s) 482, 1282
BspCNI CTCAG 2 cut(s) 322, 1547
BspFNI CGCG 1 cut(s) 1202
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 3 cut(s) 1087, 1128, 1476
BspMAI CTGCAG 1 cut(s) 457
BspMI ACCTGC 2 cut(s) 460, 503
BspPI GGATC 2 cut(s) 1121, 1134
BspQI GCTCTTC 1 cut(s) 225
BspT104I TTCGAA 1 cut(s) 912
BspTNI GGTCTC 4 cut(s) 75, 81, 993, 1235
BsrDI GCAATG 3 cut(s) 141, 1328, 1422
BsrGI TGTACA 1 cut(s) 983
BsrI ACTGG 3 cut(s) 396, 597, 1316
BssECI CCNNGG 6 cut(s) 30, 54, 83, 600, 1359, 1477
BssMI GATC 3 cut(s) 247, 988, 1126
BssNAI GTATAC 2 cut(s) 958, 1043
BssNI GRCGYC 1 cut(s) 1205
BssT1I CCWWGG 3 cut(s) 30, 600, 1359
Bst1107I GTATAC 2 cut(s) 958, 1043
Bst2UI CCWGG 1 cut(s) 1479
Bst4CI ACNGT 2 cut(s) 427, 616
Bst6I CTCTTC 4 cut(s) 84, 225, 318, 368
BstACI GRCGYC 1 cut(s) 1205
BstAPI GCANNNNNTGC 1 cut(s) 452
BstAUI TGTACA 1 cut(s) 983
BstBI TTCGAA 1 cut(s) 912
BstC8I GCNNGC 1 cut(s) 1280
BstDEI CTNAG 2 cut(s) 330, 1534
BstDSI CCRYGG 2 cut(s) 30, 600
BstF5I GGATG 1 cut(s) 1445
BstFNI CGCG 1 cut(s) 1202
BstKTI GATC 3 cut(s) 250, 991, 1129
BstMAI GTCTC 5 cut(s) 75, 81, 570, 993, 1235
BstMBI GATC 3 cut(s) 247, 988, 1126
BstMWI GCNNNNNNNGC 7 cut(s) 98, 146, 452, 689, 860, 1288, 1330
BstNI CCWGG 1 cut(s) 1479
BstSCI CCNGG 4 cut(s) 82, 83, 1263, 1477
BstSFI CTRYAG 2 cut(s) 453, 1170
BstSLI GKGCMC 1 cut(s) 895
BstUI CGCG 1 cut(s) 1202
BstV1I GCAGC 4 cut(s) 838, 1144, 1275, 1399
BstX2I RGATCY 2 cut(s) 988, 1126
BstXI CCANNNNNNTGG 1 cut(s) 470
BstYI RGATCY 2 cut(s) 988, 1126
BstZ17I GTATAC 2 cut(s) 958, 1043
BsuI GTATCC 2 cut(s) 449, 607
BsuRI GGCC 2 cut(s) 482, 1282
BtgI CCRYGG 2 cut(s) 30, 600
BtsCI GGATG 1 cut(s) 1445
BtsI GCAGTG 2 cut(s) 462, 589
BtsIMutI CAGTG 4 cut(s) 403, 462, 589, 590
BtuMI TCGCGA 1 cut(s) 1202
BveI ACCTGC 2 cut(s) 460, 503
Cac8I GCNNGC 1 cut(s) 1280
CciI TCATGA 1 cut(s) 109
Cfr13I GGNCC 3 cut(s) 1166, 1425, 1474
Cfr9I CCCGGG 1 cut(s) 83
Csp6I GTAC 4 cut(s) 652, 984, 1294, 1435
CviAII CATG 8 cut(s) 31, 50, 110, 517, 601, 841, 925, 1123
CviQI GTAC 4 cut(s) 652, 984, 1294, 1435
DdeI CTNAG 2 cut(s) 330, 1534
DpnI GATC 3 cut(s) 249, 990, 1128
DpnII GATC 3 cut(s) 247, 988, 1126
DraI TTTAAA 1 cut(s) 1354
Eam1104I CTCTTC 4 cut(s) 84, 225, 318, 368
EarI CTCTTC 4 cut(s) 84, 225, 318, 368
Eco130I CCWWGG 3 cut(s) 30, 600, 1359
Eco147I AGGCCT 1 cut(s) 1282
Eco24I GRGCYC 1 cut(s) 1112
Eco31I GGTCTC 4 cut(s) 75, 81, 993, 1235
Eco47I GGWCC 3 cut(s) 1166, 1425, 1474
Eco57I CTGAAG 1 cut(s) 684
Eco88I CYCGRG 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 1477
EcoT14I CCWWGG 3 cut(s) 30, 600, 1359
EcoT38I GRGCYC 1 cut(s) 1112
ErhI CCWWGG 3 cut(s) 30, 600, 1359
FaeI CATG 8 cut(s) 34, 53, 113, 520, 604, 844, 928, 1126
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FaqI GGGAC 3 cut(s) 57, 433, 1460
FatI CATG 8 cut(s) 30, 49, 109, 516, 600, 840, 924, 1122
FauI CCCGC 1 cut(s) 34
FbaI TGATCA 1 cut(s) 247
FblI GTMKAC 2 cut(s) 957, 1042
Fnu4HI GCNGC 5 cut(s) 852, 1158, 1289, 1413, 1466
FokI GGATG 1 cut(s) 1432
FriOI GRGCYC 1 cut(s) 1112
Fsp4HI GCNGC 5 cut(s) 852, 1158, 1289, 1413, 1466
FspBI CTAG 5 cut(s) 410, 1031, 1061, 1082, 1334
GluI GCNGC 5 cut(s) 852, 1158, 1289, 1413, 1466
HaeIII GGCC 2 cut(s) 482, 1282
HapII CCGG 2 cut(s) 84, 1265
Hin1I GRCGYC 1 cut(s) 1205
Hin1II CATG 8 cut(s) 34, 53, 113, 520, 604, 844, 928, 1126
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 4 cut(s) 467, 834, 1078, 1309
HpaII CCGG 2 cut(s) 84, 1265
Hpy166II GTNNAC 3 cut(s) 893, 958, 1043
Hpy188I TCNGA 9 cut(s) 322, 703, 770, 1094, 1249, 1275, 1400, 1429, 1537
Hpy188III TCNNGA 6 cut(s) 110, 192, 471, 992, 1066, 1201
Hpy8I GTNNAC 3 cut(s) 893, 958, 1043
Hpy99I CGWCG 1 cut(s) 1207
HpyAV CCTTC 2 cut(s) 989, 1479
HpyCH4III ACNGT 2 cut(s) 427, 616
HpyCH4IV ACGT 3 cut(s) 650, 1205, 1304
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 146, 452, 689, 860, 1288, 1330
HpyF3I CTNAG 2 cut(s) 330, 1534
HpySE526I ACGT 3 cut(s) 650, 1205, 1304
Hsp92I GRCGYC 1 cut(s) 1205
Hsp92II CATG 8 cut(s) 34, 53, 113, 520, 604, 844, 928, 1126
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 3 cut(s) 247, 988, 1126
LguI GCTCTTC 1 cut(s) 225
Lsp1109I GCAGC 4 cut(s) 838, 1144, 1275, 1399
MaeI CTAG 5 cut(s) 410, 1031, 1061, 1082, 1334
MaeII ACGT 3 cut(s) 650, 1205, 1304
MaeIII GTNAC 2 cut(s) 457, 523
MalI GATC 3 cut(s) 249, 990, 1128
MboI GATC 3 cut(s) 247, 988, 1126
MboII GAAGA 6 cut(s) 71, 212, 322, 335, 385, 690
MfeI CAATTG 2 cut(s) 884, 1023
MflI RGATCY 2 cut(s) 988, 1126
MhlI GDGCHC 2 cut(s) 895, 1112
MmeI TCCRAC 4 cut(s) 315, 375, 626, 1144
MroXI GAANNNNTTC 1 cut(s) 918
MseI TTAA 4 cut(s) 549, 813, 1301, 1353
MslI CAYNNNNRTG 2 cut(s) 605, 1454
MspA1I CMGCKG 2 cut(s) 1412, 1454
MspI CCGG 2 cut(s) 84, 1265
MspR9I CCNGG 4 cut(s) 84, 85, 1265, 1479
MunI CAATTG 2 cut(s) 884, 1023
Mva1269I GAATGC 2 cut(s) 726, 1075
MvaI CCWGG 1 cut(s) 1479
MvnI CGCG 1 cut(s) 1202
MwoI GCNNNNNNNGC 7 cut(s) 98, 146, 452, 689, 860, 1288, 1330
NciI CCSGG 3 cut(s) 84, 85, 1265
NcoI CCATGG 2 cut(s) 30, 600
NdeII GATC 3 cut(s) 247, 988, 1126
NlaIII CATG 8 cut(s) 34, 53, 113, 520, 604, 844, 928, 1126
NlaIV GGNNCC 3 cut(s) 1087, 1128, 1476
NmeAIII GCCGAG 1 cut(s) 35
NmuCI GTSAC 1 cut(s) 457
NruI TCGCGA 1 cut(s) 1202
NspV TTCGAA 1 cut(s) 912
OliI CACNNNNGTG 1 cut(s) 1454
PagI TCATGA 1 cut(s) 109
PceI AGGCCT 1 cut(s) 1282
PciSI GCTCTTC 1 cut(s) 225
PctI GAATGC 2 cut(s) 726, 1075
PdmI GAANNNNTTC 1 cut(s) 918
PfeI GAWTC 4 cut(s) 467, 834, 1078, 1309
PkrI GCNGC 5 cut(s) 853, 1159, 1290, 1414, 1467
Psp1406I AACGTT 1 cut(s) 1304
Psp6I CCWGG 1 cut(s) 1477
PspGI CCWGG 1 cut(s) 1477
PspN4I GGNNCC 3 cut(s) 1087, 1128, 1476
PspPI GGNCC 3 cut(s) 1166, 1425, 1474
PsrI GAACNNNNNNTAC 2 cut(s) 960, 992
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 2 cut(s) 988, 1126
PvuII CAGCTG 2 cut(s) 1412, 1454
RruI TCGCGA 1 cut(s) 1202
RsaI GTAC 4 cut(s) 653, 985, 1295, 1436
RsaNI GTAC 4 cut(s) 652, 984, 1294, 1435
RseI CAYNNNNRTG 2 cut(s) 605, 1454
SapI GCTCTTC 1 cut(s) 225
SaqAI TTAA 4 cut(s) 549, 813, 1301, 1353
SatI GCNGC 5 cut(s) 852, 1158, 1289, 1413, 1466
Sau3AI GATC 3 cut(s) 247, 988, 1126
Sau96I GGNCC 3 cut(s) 1166, 1425, 1474
ScrFI CCNGG 4 cut(s) 84, 85, 1265, 1479
SduI GDGCHC 2 cut(s) 895, 1112
SfcI CTRYAG 2 cut(s) 453, 1170
SfuI TTCGAA 1 cut(s) 912
SinI GGWCC 3 cut(s) 1166, 1425, 1474
SmaI CCCGGG 1 cut(s) 85
SmiMI CAYNNNNRTG 2 cut(s) 605, 1454
SmlI CTYRAG 2 cut(s) 96, 1438
SmoI CTYRAG 2 cut(s) 96, 1438
SseBI AGGCCT 1 cut(s) 1282
SsiI CCGC 4 cut(s) 41, 794, 809, 1466
SspMI CTAG 5 cut(s) 410, 1031, 1061, 1082, 1334
StuI AGGCCT 1 cut(s) 1282
StyD4I CCNGG 4 cut(s) 82, 83, 1263, 1477
StyI CCWWGG 3 cut(s) 30, 600, 1359
TaaI ACNGT 2 cut(s) 427, 616
TaiI ACGT 3 cut(s) 653, 1208, 1307
TaqI TCGA 1 cut(s) 912
TaqII GACCGA 1 cut(s) 63
TatI WGTACW 1 cut(s) 983
TauI GCSGC 1 cut(s) 1468
TfiI GAWTC 4 cut(s) 467, 834, 1078, 1309
Tru1I TTAA 4 cut(s) 549, 813, 1301, 1353
Tru9I TTAA 4 cut(s) 549, 813, 1301, 1353
TscAI CASTG 4 cut(s) 403, 462, 589, 597
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 4 cut(s) 851, 1157, 1288, 1412
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 4 cut(s) 98, 747, 790, 1434
TspMI CCCGGG 1 cut(s) 83
TspRI CASTG 4 cut(s) 403, 462, 589, 597
VneI GTGCAC 1 cut(s) 891
VpaK11BI GGWCC 3 cut(s) 1166, 1425, 1474
XapI RAATTY 5 cut(s) 121, 210, 287, 705, 715
XmaI CCCGGG 1 cut(s) 83
XmiI GTMKAC 2 cut(s) 957, 1042
XmnI GAANNNNTTC 1 cut(s) 918
XspI CTAG 5 cut(s) 410, 1031, 1061, 1082, 1334
ZraI GACGTC 1 cut(s) 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.