MD03G1163300.v1.1

Tryptophan/tyrosine permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004761
Physical Location & Seq
Forward (+)
20314545 .. 20318994
4450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1163300.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGGAAGGATATCGTTTCACAAGAGCATGTTGCCTTTCTAGAAAGTTGAAAATAGTTTGTGGCAATGGAGATATGGTGTGGTTTTGTAGGGACTTATGGGTTGGGGAATTAGTGTTGAAAGATCAGTTTGCCAGGTTCTATAATTTGTCAAATTACAACAGTTTAGCGTGGTGCAAATTGATAACAAACTCTACCGGATTGAAAGGTGTGGAGGCTATAATCTGCAGAGTTTTTCAGAGAGAAGGATATGCGTCCTATGCTTCGTACAGAAGGATATGGCTTGAATTGCAAGAGAGATACGTAAGGGAACCAACAGAGAATTGGTCATACATTTTTATACATTACGCACTTCTTGTTGCCTATGTAGCTCGTTCTTCAGAAATTTTGACAAATTTTCTCGGAATTCCCTTATGGGAAAGTGCAACCATGTTTTCAGTTCTGTTGGGAGGCATATGCTACTTCGGAAGTCAACGCTTCATTGGTGCGGTTAATGGGGTTCTGGTATCTGGAATCATCATATCTTTTGCTGCTCTTGTAGCAGTTGCAGGTGGATACCTACAATGGGATGAGCTTTTGAAGGCCAACTTTCAAGCTGTTCCCATGAGTATACCCATAATCGTTCTGTCGTTTGTTTACCAGAATGTAGTGCCTGTTCTTTGCACGGATCTTGAAGGAGACCTGTCAAAAGTAAGGACTGCAATTGTTATAGGTACAAGTATACCACTTCTTTTATTTCTAGTCTGGAATGCTGTGATTCTAGGAACCATCACAAATGTCGACATGAGCTCAGATAAGATCATGGATCCATTACAACAGTTGCAAGCTACCAATGAAGTTGTTGGACCTATTGTTGAGGTGTTCTCCCTTTTTGCTATTGCAACGTCGTACATTGGATTTGTTTTGGGCCTCTCTGATTTCCTTGCCGACTTGCTGAAACTCCCGGGTGGCCAAAGCCGGTCTCTGCCATACCTAATGACATTGGCTCCACCGGTTGTGCTGTCATTGCTAGACCCAGAAATATTCTTCAAAGCCTTGGACTTTGCTGGAACTTATGGAGTGCTGGTGCTGTTCGGAATTGTTCCAGCTGTAATGTCTTGGTCCGATAGGTACTCGTCAAGTTCATCATCCGTTGGTATGAAGTTGCCACAGTTGGTTCCAGGAGGAAGGCTTACTCTTTCACTTGTGGTTGGAGGTGCTGGATGTGTCATTTTCTCTGAAATTCTTGATAAGTTGGGCCACCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trp_Tyr_perm

Protein Analysis

415

Amino Acids

45.58

Weight (kDa)

5.83

Isoelectric Point (pI)

31.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trp_Tyr_perm PF03222 107 - 399 5.8e-52 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 536
Acc36I ACCTGC 1 cut(s) 536
AccI GTMKAC 3 cut(s) 607, 718, 777
AciI CCGC 1 cut(s) 485
AclWI GGATC 3 cut(s) 672, 797, 810
AcoI YGGCCR 1 cut(s) 946
AcsI RAATTY 4 cut(s) 381, 391, 402, 1218
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 4 cut(s) 266, 712, 887, 1109
AfiI CCNNNNNNNGG 1 cut(s) 562
AgeI ACCGGT 1 cut(s) 988
AgsI TTSAA 8 cut(s) 49, 118, 202, 284, 577, 590, 671, 1027
AjnI CCWGG 2 cut(s) 131, 1156
AluBI AGCT 6 cut(s) 368, 571, 593, 786, 824, 1085
AluI AGCT 6 cut(s) 368, 571, 593, 786, 824, 1085
Alw21I GWGCWC 1 cut(s) 788
Alw26I GTCTC 2 cut(s) 669, 963
AlwI GGATC 3 cut(s) 672, 797, 810
Ama87I CYCGRG 1 cut(s) 940
AoxI GGCC 4 cut(s) 579, 904, 946, 1234
ApeKI GCWGC 1 cut(s) 527
ApoI RAATTY 4 cut(s) 381, 391, 402, 1218
AsiGI ACCGGT 1 cut(s) 988
AspS9I GGNCC 4 cut(s) 842, 904, 1098, 1234
AsuC2I CCSGG 2 cut(s) 941, 942
AvaI CYCGRG 1 cut(s) 940
AvaII GGWCC 2 cut(s) 842, 1098
BalI TGGCCA 1 cut(s) 948
BamHI GGATCC 1 cut(s) 802
BanII GRGCYC 1 cut(s) 788
Bbv12I GWGCWC 1 cut(s) 788
BbvI GCAGC 1 cut(s) 514
BccI CCATC 1 cut(s) 773
BciT130I CCWGG 2 cut(s) 133, 1158
BciVI GTATCC 1 cut(s) 545
BcnI CCSGG 2 cut(s) 941, 942
BcoDI GTCTC 2 cut(s) 669, 963
BfaI CTAG 4 cut(s) 39, 737, 758, 1007
BfmI CTRYAG 1 cut(s) 223
BfuAI ACCTGC 1 cut(s) 536
BfuI GTATCC 1 cut(s) 545
BisI GCNGC 1 cut(s) 528
BlsI GCNGC 1 cut(s) 529
Bme1390I CCNGG 4 cut(s) 133, 941, 942, 1158
Bme18I GGWCC 2 cut(s) 842, 1098
BmeT110I CYCGRG 1 cut(s) 940
BmgT120I GGNCC 4 cut(s) 842, 904, 1098, 1234
BmiI GGNNCC 5 cut(s) 309, 763, 804, 984, 1155
BmrFI CCNGG 4 cut(s) 133, 941, 942, 1158
BplI GAGNNNNNCTC 2 cut(s) 845, 877
BpuMI CCSGG 2 cut(s) 941, 942
BsaAI YACGTR 1 cut(s) 301
BsaI GGTCTC 2 cut(s) 669, 963
BsaJI CCNNGG 2 cut(s) 940, 1032
BsaWI WCCGGW 2 cut(s) 194, 988
BsaXI ACNNNNNCTCC 6 cut(s) 60, 90, 967, 997, 1047, 1077
Bsc4I CCNNNNNNNGG 1 cut(s) 562
Bse118I RCCGGY 2 cut(s) 954, 988
Bse3DI GCAATG 2 cut(s) 70, 1001
BseBI CCWGG 2 cut(s) 133, 1158
BseDI CCNNGG 2 cut(s) 940, 1032
BseGI GGATG 3 cut(s) 571, 1124, 1205
BseLI CCNNNNNNNGG 1 cut(s) 562
BseMI GCAATG 2 cut(s) 70, 1001
BseMII CTCAG 1 cut(s) 801
BseXI GCAGC 1 cut(s) 514
BshFI GGCC 4 cut(s) 581, 906, 948, 1236
BshTI ACCGGT 1 cut(s) 988
BsiHKAI GWGCWC 1 cut(s) 788
BsiHKCI CYCGRG 1 cut(s) 940
BsiSI CCGG 4 cut(s) 195, 941, 955, 989
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 562
BsmAI GTCTC 2 cut(s) 669, 963
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 1 cut(s) 751
BsnI GGCC 4 cut(s) 581, 906, 948, 1236
Bso31I GGTCTC 2 cut(s) 669, 963
BsoBI CYCGRG 1 cut(s) 940
Bsp1286I GDGCHC 1 cut(s) 788
Bsp143I GATC 4 cut(s) 121, 664, 795, 802
BspACI CCGC 1 cut(s) 485
BspANI GGCC 4 cut(s) 581, 906, 948, 1236
BspCNI CTCAG 1 cut(s) 800
BspLI GGNNCC 5 cut(s) 309, 763, 804, 984, 1155
BspMAI CTGCAG 1 cut(s) 227
BspMI ACCTGC 1 cut(s) 536
BspPI GGATC 3 cut(s) 672, 797, 810
BspTNI GGTCTC 2 cut(s) 669, 963
BsrDI GCAATG 2 cut(s) 70, 1001
BsrFI RCCGGY 2 cut(s) 954, 988
BssAI RCCGGY 2 cut(s) 954, 988
BssECI CCNNGG 2 cut(s) 940, 1032
BssMI GATC 4 cut(s) 121, 664, 795, 802
BssNAI GTATAC 2 cut(s) 608, 719
BssT1I CCWWGG 1 cut(s) 1032
Bst1107I GTATAC 2 cut(s) 608, 719
Bst2UI CCWGG 2 cut(s) 133, 1158
Bst4CI ACNGT 3 cut(s) 161, 816, 1149
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 822
BstDEI CTNAG 1 cut(s) 787
BstF5I GGATG 3 cut(s) 571, 1124, 1205
BstKTI GATC 4 cut(s) 124, 667, 798, 805
BstMAI GTCTC 2 cut(s) 669, 963
BstMBI GATC 4 cut(s) 121, 664, 795, 802
BstMWI GCNNNNNNNGC 5 cut(s) 257, 286, 365, 536, 1003
BstNI CCWGG 2 cut(s) 133, 1158
BstNSI RCATGY 1 cut(s) 30
BstSCI CCNGG 4 cut(s) 131, 939, 940, 1156
BstSFI CTRYAG 1 cut(s) 223
BstSNI TACGTA 1 cut(s) 301
BstV1I GCAGC 1 cut(s) 514
BstX2I RGATCY 2 cut(s) 664, 802
BstYI RGATCY 2 cut(s) 664, 802
BstZ17I GTATAC 2 cut(s) 608, 719
BsuI GTATCC 1 cut(s) 545
BsuRI GGCC 4 cut(s) 581, 906, 948, 1236
BtsCI GGATG 3 cut(s) 571, 1124, 1205
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 1 cut(s) 822
Cfr10I RCCGGY 2 cut(s) 954, 988
Cfr13I GGNCC 4 cut(s) 842, 904, 1098, 1234
Cfr9I CCCGGG 1 cut(s) 940
CseI GACGC 1 cut(s) 240
Csp6I GTAC 4 cut(s) 265, 711, 886, 1108
CspAI ACCGGT 1 cut(s) 988
CviAII CATG 5 cut(s) 27, 427, 601, 781, 799
CviQI GTAC 4 cut(s) 265, 711, 886, 1108
DdeI CTNAG 1 cut(s) 787
DpnI GATC 4 cut(s) 123, 666, 797, 804
DpnII GATC 4 cut(s) 121, 664, 795, 802
EaeI YGGCCR 1 cut(s) 946
Ecl136II GAGCTC 1 cut(s) 786
Eco105I TACGTA 1 cut(s) 301
Eco130I CCWWGG 1 cut(s) 1032
Eco24I GRGCYC 1 cut(s) 788
Eco31I GGTCTC 2 cut(s) 669, 963
Eco32I GATATC 1 cut(s) 11
Eco47I GGWCC 2 cut(s) 842, 1098
Eco53kI GAGCTC 1 cut(s) 786
Eco57I CTGAAG 1 cut(s) 360
Eco88I CYCGRG 1 cut(s) 940
EcoICRI GAGCTC 1 cut(s) 786
EcoRI GAATTC 1 cut(s) 402
EcoRII CCWGG 2 cut(s) 131, 1156
EcoRV GATATC 1 cut(s) 11
EcoT14I CCWWGG 1 cut(s) 1032
EcoT38I GRGCYC 1 cut(s) 788
ErhI CCWWGG 1 cut(s) 1032
FaeI CATG 5 cut(s) 30, 430, 604, 784, 802
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FaqI GGGAC 1 cut(s) 104
FatI CATG 5 cut(s) 26, 426, 600, 780, 798
FauNDI CATATG 1 cut(s) 452
FblI GTMKAC 3 cut(s) 607, 718, 777
Fnu4HI GCNGC 1 cut(s) 528
FokI GGATG 3 cut(s) 578, 1111, 1212
FriOI GRGCYC 1 cut(s) 788
Fsp4HI GCNGC 1 cut(s) 528
FspBI CTAG 4 cut(s) 39, 737, 758, 1007
GluI GCNGC 1 cut(s) 528
HaeIII GGCC 4 cut(s) 581, 906, 948, 1236
HapII CCGG 4 cut(s) 195, 941, 955, 989
HgaI GACGC 1 cut(s) 240
Hin1II CATG 5 cut(s) 30, 430, 604, 784, 802
HincII GTYRAC 2 cut(s) 470, 778
HindII GTYRAC 2 cut(s) 470, 778
HinfI GANTC 2 cut(s) 510, 754
HpaII CCGG 4 cut(s) 195, 941, 955, 989
Hpy166II GTNNAC 5 cut(s) 470, 608, 634, 719, 778
Hpy188I TCNGA 9 cut(s) 237, 379, 401, 464, 790, 913, 1073, 1102, 1216
Hpy188III TCNNGA 5 cut(s) 39, 507, 668, 742, 1223
Hpy8I GTNNAC 5 cut(s) 470, 608, 634, 719, 778
Hpy99I CGWCG 1 cut(s) 886
HpyAV CCTTC 5 cut(s) 236, 264, 571, 665, 1158
HpyCH4III ACNGT 3 cut(s) 161, 816, 1149
HpyCH4IV ACGT 2 cut(s) 300, 881
HpyCH4V TGCA 9 cut(s) 174, 225, 289, 422, 545, 660, 698, 820, 878
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 286, 365, 536, 1003
HpyF3I CTNAG 1 cut(s) 787
HpySE526I ACGT 2 cut(s) 300, 881
Hsp92II CATG 5 cut(s) 30, 430, 604, 784, 802
Kzo9I GATC 4 cut(s) 121, 664, 795, 802
LmnI GCTCC 1 cut(s) 988
Lsp1109I GCAGC 1 cut(s) 514
MaeI CTAG 4 cut(s) 39, 737, 758, 1007
MaeII ACGT 2 cut(s) 300, 881
MalI GATC 4 cut(s) 123, 666, 797, 804
MboI GATC 4 cut(s) 121, 664, 795, 802
MboII GAAGA 2 cut(s) 366, 1015
MfeI CAATTG 1 cut(s) 699
MflI RGATCY 2 cut(s) 664, 802
MhlI GDGCHC 1 cut(s) 788
MlsI TGGCCA 1 cut(s) 948
MluNI TGGCCA 1 cut(s) 948
MmeI TCCRAC 2 cut(s) 820, 1168
MnlI CCTC 6 cut(s) 205, 440, 847, 917, 1154, 1184
Mox20I TGGCCA 1 cut(s) 948
MscI TGGCCA 1 cut(s) 948
MseI TTAA 1 cut(s) 489
Msp20I TGGCCA 1 cut(s) 948
MspA1I CMGCKG 1 cut(s) 1085
MspI CCGG 4 cut(s) 195, 941, 955, 989
MspR9I CCNGG 4 cut(s) 133, 941, 942, 1158
MunI CAATTG 1 cut(s) 699
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 2 cut(s) 133, 1158
MwoI GCNNNNNNNGC 5 cut(s) 257, 286, 365, 536, 1003
NciI CCSGG 2 cut(s) 941, 942
NdeI CATATG 1 cut(s) 452
NdeII GATC 4 cut(s) 121, 664, 795, 802
NlaIII CATG 5 cut(s) 30, 430, 604, 784, 802
NlaIV GGNNCC 5 cut(s) 309, 763, 804, 984, 1155
NspI RCATGY 1 cut(s) 30
PaqCI CACCTGC 1 cut(s) 536
PctI GAATGC 1 cut(s) 751
PfeI GAWTC 2 cut(s) 510, 754
PfoI TCCNGGA 1 cut(s) 1156
PinAI ACCGGT 1 cut(s) 988
PkrI GCNGC 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 301
Psp124BI GAGCTC 1 cut(s) 788
Psp6I CCWGG 2 cut(s) 131, 1156
PspGI CCWGG 2 cut(s) 131, 1156
PspN4I GGNNCC 5 cut(s) 309, 763, 804, 984, 1155
PspPI GGNCC 4 cut(s) 842, 904, 1098, 1234
PsrI GAACNNNNNNTAC 2 cut(s) 636, 668
PstI CTGCAG 1 cut(s) 227
PsuI RGATCY 2 cut(s) 664, 802
PvuII CAGCTG 1 cut(s) 1085
RsaI GTAC 4 cut(s) 266, 712, 887, 1109
RsaNI GTAC 4 cut(s) 265, 711, 886, 1108
SacI GAGCTC 1 cut(s) 788
SalI GTCGAC 1 cut(s) 776
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 1 cut(s) 528
Sau3AI GATC 4 cut(s) 121, 664, 795, 802
Sau96I GGNCC 4 cut(s) 842, 904, 1098, 1234
ScrFI CCNGG 4 cut(s) 133, 941, 942, 1158
SduI GDGCHC 1 cut(s) 788
SfcI CTRYAG 1 cut(s) 223
SinI GGWCC 2 cut(s) 842, 1098
SmaI CCCGGG 1 cut(s) 942
SnaBI TACGTA 1 cut(s) 301
SsiI CCGC 1 cut(s) 485
SspI AATATT 1 cut(s) 1020
SspMI CTAG 4 cut(s) 39, 737, 758, 1007
SstI GAGCTC 1 cut(s) 788
StyD4I CCNGG 4 cut(s) 131, 939, 940, 1156
StyI CCWWGG 1 cut(s) 1032
TaaI ACNGT 3 cut(s) 161, 816, 1149
TaiI ACGT 2 cut(s) 303, 884
TaqI TCGA 1 cut(s) 777
TfiI GAWTC 2 cut(s) 510, 754
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseI GCWGC 1 cut(s) 527
TspDTI ATGAA 4 cut(s) 466, 846, 1110, 1151
TspGWI ACGGA 2 cut(s) 677, 1117
TspMI CCCGGG 1 cut(s) 940
VpaK11BI GGWCC 2 cut(s) 842, 1098
XapI RAATTY 4 cut(s) 381, 391, 402, 1218
XbaI TCTAGA 1 cut(s) 38
XceI RCATGY 1 cut(s) 30
XcmI CCANNNNNNNNNTGG 1 cut(s) 318
XmaI CCCGGG 1 cut(s) 940
XmiI GTMKAC 3 cut(s) 607, 718, 777
XspI CTAG 4 cut(s) 39, 737, 758, 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.