MD03G1176200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010600
Physical Location & Seq
Reverse (-)
24155620 .. 24156045
426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1176200.v1.1.491

Sequence Viewer

Length: 426 bp
ATGATGAGCTCTTTAAGGACGAAGCATTTACCCACAGGATGGCTCACGATTCGATGGTACTTGCAATATTTAGGATCGTTTATGCGATTTATTTCTTCAGGCCGCTTGCATTCGGGTAGCTCAATTACCTTCTTTTCTAGCAAGTCGTCTAACATTGCATCCATGTCTGAGTCAGGAAAAAGGTATACCTTCTGCTCCAATTCCCTCAAGGTGCTTTTGTACCTTTCTTGGGTGCGAGGAGGCTCACTTTTCTTTATCTCCTTTGCTTTGGTTTTGGAGGAGATCTTGATAGGCGCGGAGGCGGTCTTGACGGGCGCAGTGCTGACAGTGAACGCTTCCTTGAGGGGTTTCTTCCCAGTCTTGTCTACATTTGGGGAGAACACCTTATCCTTCTTGAAGTCGGTGATCGGCTCTTTCTTCCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

15.61

Weight (kDa)

10.09

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 39
AccI GTMKAC 2 cut(s) 185, 365
AccII CGCG 1 cut(s) 296
AciI CCGC 3 cut(s) 103, 296, 302
AclWI GGATC 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 2 cut(s) 59, 221
AfiI CCNNNNNNNGG 2 cut(s) 39, 229
AgsI TTSAA 1 cut(s) 397
AloI GAACNNNNNNTCC 2 cut(s) 371, 403
AluBI AGCT 2 cut(s) 9, 120
AluI AGCT 2 cut(s) 9, 120
Alw21I GWGCWC 1 cut(s) 11
AlwI GGATC 1 cut(s) 82
AoxI GGCC 1 cut(s) 100
AspLEI GCGC 2 cut(s) 296, 317
AsuHPI GGTGA 1 cut(s) 415
BanII GRGCYC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 11
BccI CCATC 2 cut(s) 33, 48
BfaI CTAG 1 cut(s) 138
BglII AGATCT 1 cut(s) 282
BisI GCNGC 1 cut(s) 103
BlsI GCNGC 1 cut(s) 104
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 1 cut(s) 167
BmuI ACTGGG 1 cut(s) 350
BpuEI CTTGAG 2 cut(s) 191, 361
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 229
Bse1I ACTGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 153
BseGI GGATG 2 cut(s) 44, 158
BseLI CCNNNNNNNGG 2 cut(s) 39, 229
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 159
BseNI ACTGG 1 cut(s) 356
BseRI GAGGAG 2 cut(s) 252, 293
Bsh1236I CGCG 1 cut(s) 296
BshFI GGCC 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 11
BslI CCNNNNNNNGG 2 cut(s) 39, 229
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 3 cut(s) 74, 282, 405
BspACI CCGC 3 cut(s) 103, 296, 302
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 1 cut(s) 160
BspFNI CGCG 1 cut(s) 296
BspPI GGATC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 153
BsrI ACTGG 1 cut(s) 356
BssMI GATC 3 cut(s) 74, 282, 405
BssNAI GTATAC 1 cut(s) 186
Bst1107I GTATAC 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 328
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 1 cut(s) 168
BstF5I GGATG 2 cut(s) 44, 158
BstFNI CGCG 1 cut(s) 296
BstHHI GCGC 2 cut(s) 296, 317
BstKTI GATC 3 cut(s) 77, 285, 408
BstMBI GATC 3 cut(s) 74, 282, 405
BstUI CGCG 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
BstZ17I GTATAC 1 cut(s) 186
BsuRI GGCC 1 cut(s) 102
BtsCI GGATG 2 cut(s) 44, 158
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 2 cut(s) 324, 333
Cac8I GCNNGC 1 cut(s) 107
CfoI GCGC 2 cut(s) 296, 317
Csp6I GTAC 2 cut(s) 58, 220
CviAII CATG 1 cut(s) 163
CviJI RGCY 6 cut(s) 9, 43, 102, 120, 243, 411
CviKI_1 RGCY 6 cut(s) 9, 43, 102, 120, 243, 411
CviQI GTAC 2 cut(s) 58, 220
DdeI CTNAG 1 cut(s) 168
DpnI GATC 3 cut(s) 76, 284, 407
DpnII GATC 3 cut(s) 74, 282, 405
Ecl136II GAGCTC 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 11
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 11
FaeI CATG 1 cut(s) 166
FaiI YATR 3 cut(s) 83, 164, 186
FatI CATG 1 cut(s) 162
FblI GTMKAC 2 cut(s) 185, 365
Fnu4HI GCNGC 1 cut(s) 103
FokI GGATG 2 cut(s) 51, 145
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 103
FspBI CTAG 1 cut(s) 138
GlaI GCGC 2 cut(s) 295, 316
GluI GCNGC 1 cut(s) 103
HaeIII GGCC 1 cut(s) 102
HhaI GCGC 2 cut(s) 296, 317
Hin1II CATG 1 cut(s) 166
Hin6I GCGC 2 cut(s) 294, 315
HinP1I GCGC 2 cut(s) 294, 315
HinfI GANTC 2 cut(s) 49, 170
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 3 cut(s) 186, 331, 366
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 5 cut(s) 46, 174, 286, 307, 394
Hpy8I GTNNAC 3 cut(s) 186, 331, 366
HpyAV CCTTC 3 cut(s) 139, 199, 400
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4V TGCA 3 cut(s) 64, 109, 158
HpyF3I CTNAG 1 cut(s) 168
Hsp92II CATG 1 cut(s) 166
HspAI GCGC 2 cut(s) 294, 315
Kzo9I GATC 3 cut(s) 74, 282, 405
LmnI GCTCC 1 cut(s) 200
LpnPI CCDG 4 cut(s) 21, 84, 159, 369
LweI GCATC 1 cut(s) 167
MaeI CTAG 1 cut(s) 138
MalI GATC 3 cut(s) 76, 284, 407
MboI GATC 3 cut(s) 74, 282, 405
MboII GAAGA 3 cut(s) 87, 343, 409
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 2 cut(s) 123, 199
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 6 cut(s) 215, 230, 233, 271, 292, 336
MseI TTAA 1 cut(s) 14
Mva1269I GAATGC 1 cut(s) 109
MvnI CGCG 1 cut(s) 296
NdeII GATC 3 cut(s) 74, 282, 405
NlaIII CATG 1 cut(s) 166
PctI GAATGC 1 cut(s) 109
PfeI GAWTC 1 cut(s) 49
PflMI CCANNNNNTGG 1 cut(s) 39
PkrI GCNGC 1 cut(s) 104
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
Psp124BI GAGCTC 1 cut(s) 11
PsuI RGATCY 1 cut(s) 282
RsaI GTAC 2 cut(s) 59, 221
RsaNI GTAC 2 cut(s) 58, 220
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 1 cut(s) 103
Sau3AI GATC 3 cut(s) 74, 282, 405
SchI GAGTC 1 cut(s) 179
SduI GDGCHC 1 cut(s) 11
SetI ASST 8 cut(s) 11, 122, 131, 185, 191, 213, 225, 386
SfaNI GCATC 1 cut(s) 167
SmlI CTYRAG 2 cut(s) 206, 340
SmoI CTYRAG 2 cut(s) 206, 340
Sse9I AATT 2 cut(s) 123, 199
SsiI CCGC 3 cut(s) 103, 296, 302
SspI AATATT 1 cut(s) 68
SspMI CTAG 1 cut(s) 138
SstI GAGCTC 1 cut(s) 11
TaaI ACNGT 1 cut(s) 328
TaqI TCGA 1 cut(s) 52
TasI AATT 2 cut(s) 123, 199
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 1 cut(s) 49
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TscAI CASTG 2 cut(s) 324, 333
TspRI CASTG 2 cut(s) 324, 333
Van91I CCANNNNNTGG 1 cut(s) 39
XmiI GTMKAC 2 cut(s) 185, 365
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.