pycom420g00120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0010600
Physical Location & Seq
Reverse (-)
111424 .. 112172
749 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom420g00120.2

Sequence Viewer

Length: 477 bp
ATGATGAGTTCTTTAAGAACGAAGCACTTGCCAACAGGATGGCTCACAATACGATGGTACTTGCAGTACCTAGGATCGTTTGTGCGATTCATTTCTTCAGGACGCTTGCATTCGGGTAGCTCAATCACTTTTTTTTCCAGCAAGTCATCTAACATGGCAGCCATGTCAGAGTCAGGAAAAGGGTACGTTTTCTGTTCCAACTCTCTCAAGGTGCTCTTGTATCTGTCTTGGGTGCGAGAAGGCTCACTTGTCTTTATCTTCTTCGCTTTCTTCTTGGAGGAGATCTTGATGGGCGCTGAGGAGGTTTTGATGGGCGCGGAGGAGGTCTTGATGGGGGTAGTGTTGACGGTGAAAGCTGCCTTAGCGGGCTTCTTCCCGGTCTTGTCTACATTTGGGGCGAATACTTTATCCTTATTGAAGTTTGTAATCGGCTCCTTTTTCCCTGCTGTAGAAACGGTTGAGGAATTCCCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.54

Weight (kDa)

6.73

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 386
AccII CGCG 1 cut(s) 317
AciI CCGC 2 cut(s) 317, 365
AclWI GGATC 1 cut(s) 82
AcsI RAATTY 1 cut(s) 464
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 3 cut(s) 59, 68, 185
AgsI TTSAA 1 cut(s) 418
AluBI AGCT 2 cut(s) 120, 356
AluI AGCT 2 cut(s) 120, 356
Alw21I GWGCWC 1 cut(s) 216
AlwI GGATC 1 cut(s) 82
ApeKI GCWGC 2 cut(s) 158, 356
ApoI RAATTY 1 cut(s) 464
AspA2I CCTAGG 1 cut(s) 70
AspLEI GCGC 2 cut(s) 296, 317
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 361
AvrII CCTAGG 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 216
BbvCI CCTCAGC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 170, 343
BccI CCATC 5 cut(s) 33, 48, 283, 304, 325
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BcnI CCSGG 1 cut(s) 377
BfaI CTAG 2 cut(s) 71, 475
BfmI CTRYAG 1 cut(s) 447
BfoI RGCGCY 1 cut(s) 297
BglII AGATCT 1 cut(s) 282
BisI GCNGC 2 cut(s) 159, 357
BlnI CCTAGG 1 cut(s) 70
BlsI GCNGC 2 cut(s) 160, 358
Bme1390I CCNGG 1 cut(s) 377
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 1 cut(s) 377
Bpu10I CCTNAGC 2 cut(s) 297, 361
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 70
BseGI GGATG 1 cut(s) 44
BseMII CTCAG 1 cut(s) 288
BseRI GAGGAG 3 cut(s) 293, 314, 335
BseXI GCAGC 2 cut(s) 170, 343
Bsh1236I CGCG 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 1 cut(s) 377
BsmI GAATGC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 74, 282
BspACI CCGC 2 cut(s) 317, 365
BspCNI CTCAG 1 cut(s) 289
BspFNI CGCG 1 cut(s) 317
BspLI GGNNCC 1 cut(s) 433
BspPI GGATC 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 2 cut(s) 74, 282
BssT1I CCWWGG 1 cut(s) 70
Bst4CI ACNGT 2 cut(s) 349, 457
BstC8I GCNNGC 2 cut(s) 107, 367
BstDEI CTNAG 2 cut(s) 297, 361
BstF5I GGATG 1 cut(s) 44
BstFNI CGCG 1 cut(s) 317
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 2 cut(s) 296, 317
BstKTI GATC 2 cut(s) 77, 285
BstMBI GATC 2 cut(s) 74, 282
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 375
BstSFI CTRYAG 1 cut(s) 447
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 2 cut(s) 170, 343
BstX2I RGATCY 1 cut(s) 282
BstXI CCANNNNNNTGG 1 cut(s) 39
BstYI RGATCY 1 cut(s) 282
BtsCI GGATG 1 cut(s) 44
Cac8I GCNNGC 2 cut(s) 107, 367
CfoI GCGC 2 cut(s) 296, 317
CseI GACGC 1 cut(s) 111
Csp6I GTAC 3 cut(s) 58, 67, 184
CviAII CATG 2 cut(s) 154, 163
CviJI RGCY 7 cut(s) 43, 120, 161, 243, 356, 369, 432
CviKI_1 RGCY 7 cut(s) 43, 120, 161, 243, 356, 369, 432
CviQI GTAC 3 cut(s) 58, 67, 184
DdeI CTNAG 2 cut(s) 297, 361
DpnI GATC 2 cut(s) 76, 284
DpnII GATC 2 cut(s) 74, 282
Eco130I CCWWGG 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 81
EcoRI GAATTC 1 cut(s) 464
EcoT14I CCWWGG 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 2 cut(s) 157, 166
FaiI YATR 2 cut(s) 155, 164
FalI AAGNNNNNCTT 4 cut(s) 200, 232, 231, 263
FatI CATG 2 cut(s) 153, 162
FauI CCCGC 1 cut(s) 358
FblI GTMKAC 1 cut(s) 386
Fnu4HI GCNGC 2 cut(s) 159, 357
FokI GGATG 1 cut(s) 51
Fsp4HI GCNGC 2 cut(s) 159, 357
FspBI CTAG 2 cut(s) 71, 475
GlaI GCGC 2 cut(s) 295, 316
GluI GCNGC 2 cut(s) 159, 357
HaeII RGCGCY 1 cut(s) 297
HapII CCGG 1 cut(s) 377
HgaI GACGC 1 cut(s) 111
HhaI GCGC 2 cut(s) 296, 317
Hin1II CATG 2 cut(s) 157, 166
Hin6I GCGC 2 cut(s) 294, 315
HinP1I GCGC 2 cut(s) 294, 315
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HinfI GANTC 2 cut(s) 87, 170
HpaII CCGG 1 cut(s) 377
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 2 cut(s) 345, 387
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 4 cut(s) 99, 174, 286, 328
Hpy8I GTNNAC 2 cut(s) 345, 387
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 2 cut(s) 349, 457
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 64, 109
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 2 cut(s) 297, 361
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 2 cut(s) 157, 166
HspAI GCGC 2 cut(s) 294, 315
Kzo9I GATC 2 cut(s) 74, 282
LmnI GCTCC 1 cut(s) 437
LpnPI CCDG 6 cut(s) 21, 84, 151, 159, 390, 456
Lsp1109I GCAGC 2 cut(s) 170, 343
MaeI CTAG 2 cut(s) 71, 475
MaeII ACGT 1 cut(s) 186
MalI GATC 2 cut(s) 76, 284
MboI GATC 2 cut(s) 74, 282
MboII GAAGA 5 cut(s) 87, 250, 253, 262, 364
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 1 cut(s) 464
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 6 cut(s) 271, 292, 295, 313, 316, 454
MseI TTAA 1 cut(s) 14
MspI CCGG 1 cut(s) 377
MspR9I CCNGG 1 cut(s) 377
Mva1269I GAATGC 1 cut(s) 109
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 1 cut(s) 362
NciI CCSGG 1 cut(s) 377
NdeII GATC 2 cut(s) 74, 282
NlaIII CATG 2 cut(s) 157, 166
NlaIV GGNNCC 1 cut(s) 433
PctI GAATGC 1 cut(s) 109
PfeI GAWTC 1 cut(s) 87
PkrI GCNGC 2 cut(s) 160, 358
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PspN4I GGNNCC 1 cut(s) 433
PsuI RGATCY 1 cut(s) 282
RsaI GTAC 3 cut(s) 59, 68, 185
RsaNI GTAC 3 cut(s) 58, 67, 184
SaqAI TTAA 1 cut(s) 14
SatI GCNGC 2 cut(s) 159, 357
Sau3AI GATC 2 cut(s) 74, 282
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 1 cut(s) 216
SetI ASST 7 cut(s) 72, 122, 189, 213, 306, 327, 358
SfcI CTRYAG 1 cut(s) 447
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 1 cut(s) 464
SsiI CCGC 2 cut(s) 317, 365
SspMI CTAG 2 cut(s) 71, 475
StyD4I CCNGG 1 cut(s) 375
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 2 cut(s) 349, 457
TaiI ACGT 1 cut(s) 189
TasI AATT 1 cut(s) 464
TfiI GAWTC 1 cut(s) 87
Tru1I TTAA 1 cut(s) 14
Tru9I TTAA 1 cut(s) 14
TseI GCWGC 2 cut(s) 158, 356
TspDTI ATGAA 1 cut(s) 79
XapI RAATTY 1 cut(s) 464
XmaJI CCTAGG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 386
XspI CTAG 2 cut(s) 71, 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.