pycom04g10800

Choline monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010600
Physical Location & Seq
Reverse (-)
13573838 .. 13574365
528 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g10800.1

Sequence Viewer

Length: 528 bp
ATGAGCGTGGTAGCTCAGTCCACCTCCAGCTTCTATTCGGCTTTCTCCACCAGAGGCTCCTCCTTTTCGTCAGTTTCCTCCTTTGGTGGATCAACCTTCGGGTTAGCTTTCTCATTGTGCTACGCCTCCAGACGGTTGACAAGTGTAACAATTTGCAAGTCTTTTTCCTCCACAGTCTTTGTCAACCTTGCGATTGCTTCGCTCATTTATGCCAACTGCTCTTCAATTGAAGTCACACCAGTAGTCATGACTTGCATGCCTACAGGATACAAGCTGCTGCTTAAGTCGGCATCGAAAGTCAACAACTCGGAGTACCTCCTCGGGCTTTCTTCCCTTGGTGCCCTTAGCGAGGCCAAGGTGATCACGAGCTTGTGCCTCAGGTGCTCTTGCTCATTCGGCAAAGTTGATGTAAGGTTGGAAGGCACGGCAGAGAGAGCTTTTGCCTTGCTTCGAGTTGTGACGCCCAAAGTGCCACCACTTGCGGCAATGATGTTTTTGTTCTTCGCGTTGGTTGCGGGAACAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 map00260
Pfam Domains
Protein Families
Rieske Ring_hydroxyl_A

Protein Analysis

176

Amino Acids

18.47

Weight (kDa)

9.34

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 338
AccII CGCG 1 cut(s) 506
AciI CCGC 2 cut(s) 482, 515
AclWI GGATC 1 cut(s) 97
AcyI GRCGYC 1 cut(s) 461
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 2 cut(s) 132, 349
AflII CTTAAG 1 cut(s) 281
AgsI TTSAA 2 cut(s) 225, 230
AluBI AGCT 6 cut(s) 14, 30, 107, 274, 369, 437
AluI AGCT 6 cut(s) 14, 30, 107, 274, 369, 437
Alw21I GWGCWC 1 cut(s) 386
AlwI GGATC 1 cut(s) 97
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 1 cut(s) 351
ApeKI GCWGC 2 cut(s) 274, 277
AsuHPI GGTGA 1 cut(s) 370
AvaI CYCGRG 1 cut(s) 320
AxyI CCTNAGG 1 cut(s) 377
BaeGI GKGCMC 1 cut(s) 343
BanI GGYRCC 1 cut(s) 338
BauI CACGAG 1 cut(s) 364
Bbv12I GWGCWC 1 cut(s) 386
BbvI GCAGC 2 cut(s) 261, 264
BceAI ACGGC 1 cut(s) 441
BciVI GTATCC 1 cut(s) 260
BclI TGATCA 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 261
BfrI CTTAAG 1 cut(s) 281
BfuI GTATCC 1 cut(s) 260
BisI GCNGC 3 cut(s) 275, 278, 483
BlsI GCNGC 3 cut(s) 276, 279, 484
BmeT110I CYCGRG 1 cut(s) 320
BmiI GGNNCC 2 cut(s) 58, 340
BmsI GCATC 1 cut(s) 299
BpmI CTGGAG 2 cut(s) 10, 112
Bpu10I CCTNAGC 1 cut(s) 344
BsaHI GRCGYC 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 319, 334, 354
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 349
Bse1I ACTGG 1 cut(s) 239
Bse21I CCTNAGG 1 cut(s) 377
Bse3DI GCAATG 1 cut(s) 492
BseDI CCNNGG 3 cut(s) 319, 334, 354
BseLI CCNNNNNNNGG 2 cut(s) 132, 349
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 2 cut(s) 29, 391
BseNI ACTGG 1 cut(s) 239
BseRI GAGGAG 2 cut(s) 49, 308
BseSI GKGCMC 1 cut(s) 343
BseXI GCAGC 2 cut(s) 261, 264
Bsh1236I CGCG 1 cut(s) 506
BshFI GGCC 1 cut(s) 353
BshNI GGYRCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 386
BsiHKCI CYCGRG 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 132, 349
BsnI GGCC 1 cut(s) 353
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 2 cut(s) 343, 386
Bsp143I GATC 2 cut(s) 89, 360
BspACI CCGC 2 cut(s) 482, 515
BspANI GGCC 1 cut(s) 353
BspCNI CTCAG 2 cut(s) 28, 390
BspFNI CGCG 1 cut(s) 506
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 58, 340
BspPI GGATC 1 cut(s) 97
BspQI GCTCTTC 1 cut(s) 226
BspT107I GGYRCC 1 cut(s) 338
BspTI CTTAAG 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 492
BsrI ACTGG 1 cut(s) 239
BssECI CCNNGG 3 cut(s) 319, 334, 354
BssMI GATC 2 cut(s) 89, 360
BssNI GRCGYC 1 cut(s) 461
BssSI CACGAG 1 cut(s) 364
BssT1I CCWWGG 2 cut(s) 334, 354
Bst2BI CACGAG 1 cut(s) 364
Bst4CI ACNGT 2 cut(s) 135, 175
Bst6I CTCTTC 1 cut(s) 226
BstACI GRCGYC 1 cut(s) 461
BstAFI CTTAAG 1 cut(s) 281
BstC8I GCNNGC 1 cut(s) 257
BstDEI CTNAG 3 cut(s) 15, 344, 377
BstENI CCTNNNNNAGG 1 cut(s) 347
BstFNI CGCG 1 cut(s) 506
BstKTI GATC 2 cut(s) 92, 363
BstMBI GATC 2 cut(s) 89, 360
BstMWI GCNNNNNNNGC 5 cut(s) 381, 396, 434, 469, 512
BstNSI RCATGY 1 cut(s) 259
BstSFI CTRYAG 1 cut(s) 261
BstSLI GKGCMC 1 cut(s) 343
BstUI CGCG 1 cut(s) 506
BstV1I GCAGC 2 cut(s) 261, 264
Bsu36I CCTNAGG 1 cut(s) 377
BsuI GTATCC 1 cut(s) 260
BsuRI GGCC 1 cut(s) 353
Cac8I GCNNGC 1 cut(s) 257
CciI TCATGA 1 cut(s) 246
CseI GACGC 1 cut(s) 469
Csp6I GTAC 1 cut(s) 313
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 2 cut(s) 247, 256
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 3 cut(s) 15, 344, 377
DpnI GATC 2 cut(s) 91, 362
DpnII GATC 2 cut(s) 89, 360
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Eco130I CCWWGG 2 cut(s) 334, 354
Eco81I CCTNAGG 1 cut(s) 377
Eco88I CYCGRG 1 cut(s) 320
EcoNI CCTNNNNNAGG 1 cut(s) 347
EcoT14I CCWWGG 2 cut(s) 334, 354
ErhI CCWWGG 2 cut(s) 334, 354
FaeI CATG 2 cut(s) 250, 259
FaiI YATR 3 cut(s) 210, 248, 257
FatI CATG 2 cut(s) 246, 255
FauI CCCGC 1 cut(s) 508
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 3 cut(s) 275, 278, 483
Fsp4HI GCNGC 3 cut(s) 275, 278, 483
GluI GCNGC 3 cut(s) 275, 278, 483
GsuI CTGGAG 2 cut(s) 10, 112
HaeIII GGCC 1 cut(s) 353
HgaI GACGC 1 cut(s) 469
Hin1I GRCGYC 1 cut(s) 461
Hin1II CATG 2 cut(s) 250, 259
HincII GTYRAC 3 cut(s) 138, 184, 301
HindII GTYRAC 3 cut(s) 138, 184, 301
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 4 cut(s) 21, 138, 184, 301
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 3 cut(s) 129, 247, 364
Hpy8I GTNNAC 4 cut(s) 21, 138, 184, 301
HpyAV CCTTC 2 cut(s) 106, 413
HpyCH4III ACNGT 2 cut(s) 135, 175
HpyCH4V TGCA 2 cut(s) 156, 255
HpyF10VI GCNNNNNNNGC 5 cut(s) 381, 396, 434, 469, 512
HpyF3I CTNAG 3 cut(s) 15, 344, 377
Hsp92I GRCGYC 1 cut(s) 461
Hsp92II CATG 2 cut(s) 250, 259
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 2 cut(s) 89, 360
LguI GCTCTTC 1 cut(s) 226
LmnI GCTCC 1 cut(s) 62
LpnPI CCDG 6 cut(s) 40, 64, 142, 249, 252, 364
Lsp1109I GCAGC 2 cut(s) 261, 264
LweI GCATC 1 cut(s) 299
MaeIII GTNAC 3 cut(s) 145, 232, 457
MalI GATC 2 cut(s) 91, 362
MboI GATC 2 cut(s) 89, 360
MboII GAAGA 3 cut(s) 213, 321, 493
MfeI CAATTG 1 cut(s) 225
MhlI GDGCHC 2 cut(s) 343, 386
MluCI AATT 2 cut(s) 150, 225
MmeI TCCRAC 1 cut(s) 396
MseI TTAA 1 cut(s) 282
MspCI CTTAAG 1 cut(s) 281
MunI CAATTG 1 cut(s) 225
MvnI CGCG 1 cut(s) 506
MwoI GCNNNNNNNGC 5 cut(s) 381, 396, 434, 469, 512
NdeII GATC 2 cut(s) 89, 360
NlaIII CATG 2 cut(s) 250, 259
NlaIV GGNNCC 2 cut(s) 58, 340
NmuCI GTSAC 2 cut(s) 232, 457
NspI RCATGY 1 cut(s) 259
PaeI GCATGC 1 cut(s) 259
PagI TCATGA 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 226
PkrI GCNGC 3 cut(s) 276, 279, 484
PspN4I GGNNCC 2 cut(s) 58, 340
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SapI GCTCTTC 1 cut(s) 226
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 3 cut(s) 275, 278, 483
Sau3AI GATC 2 cut(s) 89, 360
SduI GDGCHC 2 cut(s) 343, 386
SfaNI GCATC 1 cut(s) 299
SfcI CTRYAG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SphI GCATGC 1 cut(s) 259
Sse9I AATT 2 cut(s) 150, 225
SsiI CCGC 2 cut(s) 482, 515
StyI CCWWGG 2 cut(s) 334, 354
TaaI ACNGT 2 cut(s) 135, 175
TaqI TCGA 2 cut(s) 293, 451
TasI AATT 2 cut(s) 150, 225
TauI GCSGC 1 cut(s) 485
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TseFI GTSAC 2 cut(s) 232, 457
TseI GCWGC 2 cut(s) 274, 277
Tsp45I GTSAC 2 cut(s) 232, 457
Vha464I CTTAAG 1 cut(s) 281
XagI CCTNNNNNAGG 1 cut(s) 347
XceI RCATGY 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.