pycom13g19210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010600
Physical Location & Seq
Reverse (-)
15225452 .. 15225898
447 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g19210.1

Sequence Viewer

Length: 447 bp
ATGATGAGTTCTTTGAGAACGAAGCACTTGCCAACAGGATGGCTCACAATACGATGGTACTTGCAGTACCTAGGATCGTTTGTGCGATTCATTTCTTCAGGACGCTTGCATTCGGGTAGCTCAATCACCTTTTTTTCCAGCAAGTCATCTAACATGGCAGCCATGTCAGAGTCAGGAAAAGGGTACGTTTTCTGTTCCAACTCTCTCAAGGTGCTCTTGTATCTGTCTTGGGTGCGAGAAGGCTCACTTGTCTTTATCTCCTTCGCTTTCTTCTTGGAGGAGATCTTGATGGGCGCTGAGGAGGTCTTGATGGGCGCTGAGGAGGTCTTGATGGGGGTAGTGTTGACGGTGAAAGCTTCCTTAGCGGGCTTCTTCCCGGTCTTGTCTACATTTGGGGCGAATACTTTCTCCTTATTGAAGTTAGTAATTGGCTCCTTTTTCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.34

Weight (kDa)

9.21

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 386
AciI CCGC 1 cut(s) 365
AclWI GGATC 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 3 cut(s) 59, 68, 185
AgsI TTSAA 1 cut(s) 418
AluBI AGCT 2 cut(s) 120, 356
AluI AGCT 2 cut(s) 120, 356
Alw21I GWGCWC 1 cut(s) 216
AlwI GGATC 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 404
AspA2I CCTAGG 1 cut(s) 70
AspLEI GCGC 2 cut(s) 296, 317
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 2 cut(s) 118, 361
AvrII CCTAGG 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 216
BbvCI CCTCAGC 2 cut(s) 297, 318
BbvI GCAGC 1 cut(s) 170
BccI CCATC 5 cut(s) 33, 48, 283, 304, 325
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BcnI CCSGG 1 cut(s) 377
BfaI CTAG 1 cut(s) 71
BfoI RGCGCY 2 cut(s) 297, 318
BglII AGATCT 1 cut(s) 282
BisI GCNGC 1 cut(s) 159
BlnI CCTAGG 1 cut(s) 70
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 377
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 1 cut(s) 377
Bpu10I CCTNAGC 3 cut(s) 297, 318, 361
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 70
BseGI GGATG 1 cut(s) 44
BseMII CTCAG 2 cut(s) 288, 309
BseRI GAGGAG 3 cut(s) 293, 314, 335
BseXI GCAGC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 1 cut(s) 377
BsmI GAATGC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 74, 282
BspACI CCGC 1 cut(s) 365
BspCNI CTCAG 2 cut(s) 289, 310
BspLI GGNNCC 1 cut(s) 433
BspPI GGATC 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 2 cut(s) 74, 282
BssT1I CCWWGG 1 cut(s) 70
Bst4CI ACNGT 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 107, 367
BstDEI CTNAG 3 cut(s) 297, 318, 361
BstF5I GGATG 1 cut(s) 44
BstH2I RGCGCY 2 cut(s) 297, 318
BstHHI GCGC 2 cut(s) 296, 317
BstKTI GATC 2 cut(s) 77, 285
BstMBI GATC 2 cut(s) 74, 282
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 282
BstXI CCANNNNNNTGG 1 cut(s) 39
BstYI RGATCY 1 cut(s) 282
BtsCI GGATG 1 cut(s) 44
Cac8I GCNNGC 2 cut(s) 107, 367
CfoI GCGC 2 cut(s) 296, 317
CseI GACGC 1 cut(s) 111
Csp6I GTAC 3 cut(s) 58, 67, 184
CviAII CATG 3 cut(s) 154, 163, 444
CviJI RGCY 7 cut(s) 43, 120, 161, 243, 356, 369, 432
CviKI_1 RGCY 7 cut(s) 43, 120, 161, 243, 356, 369, 432
CviQI GTAC 3 cut(s) 58, 67, 184
DdeI CTNAG 3 cut(s) 297, 318, 361
DpnI GATC 2 cut(s) 76, 284
DpnII GATC 2 cut(s) 74, 282
Eco130I CCWWGG 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 3 cut(s) 157, 166, 447
FaiI YATR 3 cut(s) 155, 164, 445
FalI AAGNNNNNCTT 4 cut(s) 200, 232, 231, 263
FatI CATG 3 cut(s) 153, 162, 443
FauI CCCGC 1 cut(s) 358
FblI GTMKAC 1 cut(s) 386
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 1 cut(s) 51
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 71
GlaI GCGC 2 cut(s) 295, 316
GluI GCNGC 1 cut(s) 159
HaeII RGCGCY 2 cut(s) 297, 318
HapII CCGG 1 cut(s) 377
HgaI GACGC 1 cut(s) 111
HhaI GCGC 2 cut(s) 296, 317
Hin1II CATG 3 cut(s) 157, 166, 447
Hin6I GCGC 2 cut(s) 294, 315
HinP1I GCGC 2 cut(s) 294, 315
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 2 cut(s) 87, 170
HpaII CCGG 1 cut(s) 377
HphI GGTGA 2 cut(s) 118, 361
Hpy166II GTNNAC 2 cut(s) 345, 387
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 5 cut(s) 99, 174, 286, 307, 328
Hpy8I GTNNAC 2 cut(s) 345, 387
HpyAV CCTTC 2 cut(s) 233, 271
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 64, 109
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 3 cut(s) 297, 318, 361
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 3 cut(s) 157, 166, 447
HspAI GCGC 2 cut(s) 294, 315
Kzo9I GATC 2 cut(s) 74, 282
LmnI GCTCC 1 cut(s) 437
LpnPI CCDG 5 cut(s) 21, 84, 151, 159, 390
Lsp1109I GCAGC 1 cut(s) 170
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 186
MalI GATC 2 cut(s) 76, 284
MboI GATC 2 cut(s) 74, 282
MboII GAAGA 3 cut(s) 87, 262, 364
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 1 cut(s) 426
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 5 cut(s) 271, 292, 295, 313, 316
MroXI GAANNNNTTC 1 cut(s) 404
MspI CCGG 1 cut(s) 377
MspR9I CCNGG 1 cut(s) 377
Mva1269I GAATGC 1 cut(s) 109
MwoI GCNNNNNNNGC 1 cut(s) 362
NciI CCSGG 1 cut(s) 377
NdeII GATC 2 cut(s) 74, 282
NlaIII CATG 3 cut(s) 157, 166, 447
NlaIV GGNNCC 1 cut(s) 433
PctI GAATGC 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 404
PfeI GAWTC 1 cut(s) 87
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PspN4I GGNNCC 1 cut(s) 433
PsuI RGATCY 1 cut(s) 282
RsaI GTAC 3 cut(s) 59, 68, 185
RsaNI GTAC 3 cut(s) 58, 67, 184
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 2 cut(s) 74, 282
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 1 cut(s) 216
SetI ASST 8 cut(s) 72, 122, 131, 189, 213, 306, 327, 358
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 1 cut(s) 426
SsiI CCGC 1 cut(s) 365
SspMI CTAG 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 375
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 1 cut(s) 189
TasI AATT 1 cut(s) 426
TfiI GAWTC 1 cut(s) 87
TseI GCWGC 1 cut(s) 158
TspDTI ATGAA 1 cut(s) 79
XmaJI CCTAGG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 386
XmnI GAANNNNTTC 1 cut(s) 404
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.