pycom11g04170

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010600
Physical Location & Seq
Reverse (-)
3266672 .. 3267139
468 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g04170.1

Sequence Viewer

Length: 468 bp
ATGATGAGTTCTTTGAGAACGAAGCACTTGCCAACAGGATGGCTCACAATACGATGGTACTTGCAGTACCTAGGATCGTTTGTGCGATTCATTTCTTCAGGACGCTTGCATTCGGGTAGCTCAATCACCTTTTTTTCCAGCAAGTCATCTAACATGGCAGCCATGTCAGAGTCAGGAAAAGGGTACGTTTTCTGTTCCAACTCTCTCAAGGTGCTCTTGTATCTGTCTTGGGTGCGAGAAGGCTCACTTGTCTTTATCTCCTTCGCTTTCTTCTTGGAGGAGATCTTGATGGGCGCTGAGGAGGTCTTGATGGGCGCTGAGGAGGTCTTGATGGGCGCTGAAGAGGTCTTGATGGGGGTAGTGTTGACGGTGAAAGCTTCCTTAGCTGGCTTCTTCCCGGTCTTGTCTATATTTGGGGCGAATACTTTCTCCTTATTGAAGTTAGTAATTGGCTCCTTTTTCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

17.08

Weight (kDa)

7.88

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 82
AcuI CTGAAG 2 cut(s) 81, 360
AfaI GTAC 3 cut(s) 59, 68, 185
AgsI TTSAA 1 cut(s) 439
AluBI AGCT 3 cut(s) 120, 377, 386
AluI AGCT 3 cut(s) 120, 377, 386
Alw21I GWGCWC 1 cut(s) 216
AlwI GGATC 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 425
AspA2I CCTAGG 1 cut(s) 70
AspLEI GCGC 3 cut(s) 296, 317, 338
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 2 cut(s) 118, 382
AvrII CCTAGG 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 216
BbvCI CCTCAGC 2 cut(s) 297, 318
BbvI GCAGC 1 cut(s) 170
BccI CCATC 6 cut(s) 33, 48, 283, 304, 325, 346
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BcnI CCSGG 1 cut(s) 398
BfaI CTAG 1 cut(s) 71
BfoI RGCGCY 3 cut(s) 297, 318, 339
BglII AGATCT 1 cut(s) 282
BisI GCNGC 1 cut(s) 159
BlnI CCTAGG 1 cut(s) 70
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 454
BmrFI CCNGG 1 cut(s) 398
Bpu10I CCTNAGC 3 cut(s) 297, 318, 382
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 70
BseGI GGATG 1 cut(s) 44
BseMII CTCAG 2 cut(s) 288, 309
BseRI GAGGAG 3 cut(s) 293, 314, 335
BseXI GCAGC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 1 cut(s) 398
BsmI GAATGC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 74, 282
BspCNI CTCAG 2 cut(s) 289, 310
BspLI GGNNCC 1 cut(s) 454
BspPI GGATC 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 2 cut(s) 74, 282
BssT1I CCWWGG 1 cut(s) 70
Bst4CI ACNGT 1 cut(s) 370
Bst6I CTCTTC 1 cut(s) 336
BstC8I GCNNGC 2 cut(s) 107, 388
BstDEI CTNAG 3 cut(s) 297, 318, 382
BstF5I GGATG 1 cut(s) 44
BstH2I RGCGCY 3 cut(s) 297, 318, 339
BstHHI GCGC 3 cut(s) 296, 317, 338
BstKTI GATC 2 cut(s) 77, 285
BstMBI GATC 2 cut(s) 74, 282
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstSCI CCNGG 1 cut(s) 396
BstV1I GCAGC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 282
BstXI CCANNNNNNTGG 1 cut(s) 39
BstYI RGATCY 1 cut(s) 282
BtsCI GGATG 1 cut(s) 44
Cac8I GCNNGC 2 cut(s) 107, 388
CfoI GCGC 3 cut(s) 296, 317, 338
CseI GACGC 1 cut(s) 111
Csp6I GTAC 3 cut(s) 58, 67, 184
CviAII CATG 3 cut(s) 154, 163, 465
CviJI RGCY 8 cut(s) 43, 120, 161, 243, 377, 386, 390, 453
CviKI_1 RGCY 8 cut(s) 43, 120, 161, 243, 377, 386, 390, 453
CviQI GTAC 3 cut(s) 58, 67, 184
DdeI CTNAG 3 cut(s) 297, 318, 382
DpnI GATC 2 cut(s) 76, 284
DpnII GATC 2 cut(s) 74, 282
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 70
Eco57I CTGAAG 2 cut(s) 81, 360
EcoT14I CCWWGG 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 3 cut(s) 157, 166, 468
FaiI YATR 4 cut(s) 155, 164, 410, 466
FalI AAGNNNNNCTT 4 cut(s) 200, 232, 231, 263
FatI CATG 3 cut(s) 153, 162, 464
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 1 cut(s) 51
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 71
GlaI GCGC 3 cut(s) 295, 316, 337
GluI GCNGC 1 cut(s) 159
HaeII RGCGCY 3 cut(s) 297, 318, 339
HapII CCGG 1 cut(s) 398
HgaI GACGC 1 cut(s) 111
HhaI GCGC 3 cut(s) 296, 317, 338
Hin1II CATG 3 cut(s) 157, 166, 468
Hin6I GCGC 3 cut(s) 294, 315, 336
HinP1I GCGC 3 cut(s) 294, 315, 336
HincII GTYRAC 1 cut(s) 366
HindII GTYRAC 1 cut(s) 366
HindIII AAGCTT 1 cut(s) 375
HinfI GANTC 2 cut(s) 87, 170
HpaII CCGG 1 cut(s) 398
HphI GGTGA 2 cut(s) 118, 382
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 6 cut(s) 99, 174, 286, 307, 328, 349
Hpy8I GTNNAC 1 cut(s) 366
HpyAV CCTTC 2 cut(s) 233, 271
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 64, 109
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpyF3I CTNAG 3 cut(s) 297, 318, 382
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 3 cut(s) 157, 166, 468
HspAI GCGC 3 cut(s) 294, 315, 336
Kzo9I GATC 2 cut(s) 74, 282
LmnI GCTCC 1 cut(s) 458
LpnPI CCDG 6 cut(s) 21, 84, 151, 159, 372, 411
Lsp1109I GCAGC 1 cut(s) 170
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 186
MalI GATC 2 cut(s) 76, 284
MboI GATC 2 cut(s) 74, 282
MboII GAAGA 4 cut(s) 87, 262, 353, 385
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 1 cut(s) 447
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 6 cut(s) 271, 292, 295, 313, 316, 337
MroXI GAANNNNTTC 1 cut(s) 425
MspI CCGG 1 cut(s) 398
MspR9I CCNGG 1 cut(s) 398
Mva1269I GAATGC 1 cut(s) 109
MwoI GCNNNNNNNGC 1 cut(s) 383
NciI CCSGG 1 cut(s) 398
NdeII GATC 2 cut(s) 74, 282
NlaIII CATG 3 cut(s) 157, 166, 468
NlaIV GGNNCC 1 cut(s) 454
PctI GAATGC 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 1 cut(s) 87
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PspN4I GGNNCC 1 cut(s) 454
PsuI RGATCY 1 cut(s) 282
RsaI GTAC 3 cut(s) 59, 68, 185
RsaNI GTAC 3 cut(s) 58, 67, 184
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 2 cut(s) 74, 282
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 398
SduI GDGCHC 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 1 cut(s) 447
SspMI CTAG 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 396
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 1 cut(s) 189
TasI AATT 1 cut(s) 447
TfiI GAWTC 1 cut(s) 87
TseI GCWGC 1 cut(s) 158
TspDTI ATGAA 1 cut(s) 79
XmaJI CCTAGG 1 cut(s) 70
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.