pycom13g27810

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010600
Physical Location & Seq
Forward (+)
23926728 .. 23927547
820 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g27810.2

Sequence Viewer

Length: 777 bp
ATGCAAGGAAACGGGAATGCGTGGCTCCTGCTCCATAGGTTCCCCAGTATATGTTGTCTTATCATCCTTTATGTTTCTTTTAGGCTTCCCATTGTTGCGGCCGTGGTGATTTCTTCTTTGTTCCACCTTTGGCTGTATGACTCGTGGTCTTGGCTTCCTTGTTTTTTTGTAGGTTACCAATATCCATCCTTCTTCATCATCGGAGGTACTGTGTCGCTTGAGCATTCACGGGAATGGTCAGGTGTCGCTTGGAGAGGCACGGGATCGAACGATCCAAACGCAATTGTAGTGGTATGCGTTGCAGCCGTGTCCTCAAGGTCGAGCACAATTTGCCCTTGTTGTGCTAGCTTCATAATGAGTTCTTTAAGAACGAAGCACTTGCCAACAGGATGGTTCATAATACGATGGTACTTGCGGTACCTAGGATCGTCTATGCGATTCATTTCTTCAGGACGCTTGCATTCGGGTAGCTCAATCACCTTTTTTTCCAGCAAGTCATCTAACATAGCAGCCATGTCAGAGTCAGGAAAATGGTACGTTTTCTGTTCCAACTCTCTCAAGGTGTTCTTGTATCTGTATTGGGTGCGAGAAGGCTCACCTTTCTTTATCTCCTTCGCTTTCTTTTTGGAGGAGATCTTGATGGGCGCTGAGGAGGTCTTAATGGGGGTAGTGTTGACGGTGAAAGCTTCCTTAGCGGGCTTCTTCCCAATCTTGTCTACATTTGGGGTGAATACTTTATCCTTCTTGAAGTTAGTAATCGGCTCATTTTTCCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

28.89

Weight (kDa)

9.08

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 417
AccB1I GGYRCC 1 cut(s) 417
AccI GTMKAC 1 cut(s) 716
AciI CCGC 3 cut(s) 98, 415, 695
AclWI GGATC 3 cut(s) 266, 271, 433
AcoI YGGCCR 1 cut(s) 99
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 4 cut(s) 208, 410, 419, 536
AgsI TTSAA 1 cut(s) 748
AhdI GACNNNNNGTC 1 cut(s) 145
AluBI AGCT 3 cut(s) 348, 471, 686
AluI AGCT 3 cut(s) 348, 471, 686
Alw21I GWGCWC 1 cut(s) 326
AlwI GGATC 3 cut(s) 266, 271, 433
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 2 cut(s) 302, 509
Asp718I GGTACC 1 cut(s) 417
AspA2I CCTAGG 1 cut(s) 421
AspLEI GCGC 1 cut(s) 647
AsuHPI GGTGA 5 cut(s) 118, 469, 588, 691, 739
AsuNHI GCTAGC 1 cut(s) 344
AvrII CCTAGG 1 cut(s) 421
BanI GGYRCC 1 cut(s) 417
BauI CACGAG 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 326
BbvCI CCTCAGC 1 cut(s) 648
BbvI GCAGC 2 cut(s) 314, 521
BccI CCATC 4 cut(s) 193, 384, 399, 634
BceAI ACGGC 2 cut(s) 86, 290
BcgI CGANNNNNNTGC 2 cut(s) 361, 395
BfaI CTAG 2 cut(s) 345, 422
BfoI RGCGCY 1 cut(s) 648
BglII AGATCT 1 cut(s) 633
BisI GCNGC 3 cut(s) 99, 303, 510
BlnI CCTAGG 1 cut(s) 421
BlsI GCNGC 3 cut(s) 100, 304, 511
BmeRI GACNNNNNGTC 1 cut(s) 145
BmiI GGNNCC 3 cut(s) 26, 41, 419
BmrI ACTGGG 1 cut(s) 39
BmtI GCTAGC 1 cut(s) 348
BmuI ACTGGG 1 cut(s) 39
Bpu10I CCTNAGC 2 cut(s) 648, 691
BpuEI CTTGAG 3 cut(s) 239, 298, 542
BsaJI CCNNGG 2 cut(s) 102, 421
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bse1I ACTGG 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 102, 421
BseGI GGATG 3 cut(s) 63, 185, 395
BseMII CTCAG 1 cut(s) 639
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 2 cut(s) 644, 665
BseX3I CGGCCG 1 cut(s) 99
BseXI GCAGC 2 cut(s) 314, 521
Bsh1285I CGRYCG 1 cut(s) 102
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 417
BsiEI CGRYCG 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 326
BsmI GAATGC 3 cut(s) 22, 223, 460
BsnI GGCC 1 cut(s) 101
Bsp1286I GDGCHC 1 cut(s) 326
Bsp143I GATC 4 cut(s) 263, 271, 425, 633
BspACI CCGC 3 cut(s) 98, 415, 695
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 1 cut(s) 640
BspLI GGNNCC 3 cut(s) 26, 41, 419
BspOI GCTAGC 1 cut(s) 348
BspPI GGATC 3 cut(s) 266, 271, 433
BspT107I GGYRCC 1 cut(s) 417
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 102, 421
BssMI GATC 4 cut(s) 263, 271, 425, 633
BssSI CACGAG 1 cut(s) 142
BssT1I CCWWGG 1 cut(s) 421
Bst2BI CACGAG 1 cut(s) 142
Bst4CI ACNGT 2 cut(s) 211, 679
BstAPI GCANNNNNTGC 1 cut(s) 330
BstC8I GCNNGC 3 cut(s) 346, 458, 697
BstDEI CTNAG 2 cut(s) 648, 691
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 1 cut(s) 173
BstF5I GGATG 3 cut(s) 63, 185, 395
BstH2I RGCGCY 1 cut(s) 648
BstHHI GCGC 1 cut(s) 647
BstKTI GATC 4 cut(s) 266, 274, 428, 636
BstMBI GATC 4 cut(s) 263, 271, 425, 633
BstMCI CGRYCG 1 cut(s) 102
BstMWI GCNNNNNNNGC 2 cut(s) 330, 692
BstPI GGTNACC 1 cut(s) 173
BstV1I GCAGC 2 cut(s) 314, 521
BstX2I RGATCY 1 cut(s) 633
BstXI CCANNNNNNTGG 1 cut(s) 390
BstYI RGATCY 1 cut(s) 633
BstZI CGGCCG 1 cut(s) 99
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 63, 185, 395
Cac8I GCNNGC 3 cut(s) 346, 458, 697
CfoI GCGC 1 cut(s) 647
CseI GACGC 1 cut(s) 462
Csp6I GTAC 4 cut(s) 207, 409, 418, 535
CspCI CAANNNNNGTGG 2 cut(s) 270, 305
CviAII CATG 1 cut(s) 514
CviQI GTAC 4 cut(s) 207, 409, 418, 535
DdeI CTNAG 2 cut(s) 648, 691
DpnI GATC 4 cut(s) 265, 273, 427, 635
DpnII GATC 4 cut(s) 263, 271, 425, 633
DriI GACNNNNNGTC 1 cut(s) 145
EaeI YGGCCR 1 cut(s) 99
EagI CGGCCG 1 cut(s) 99
Eam1105I GACNNNNNGTC 1 cut(s) 145
EclXI CGGCCG 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 421
Eco52I CGGCCG 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 432
Eco91I GGTNACC 1 cut(s) 173
EcoO65I GGTNACC 1 cut(s) 173
EcoT14I CCWWGG 1 cut(s) 421
ErhI CCWWGG 1 cut(s) 421
FaeI CATG 1 cut(s) 517
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FatI CATG 1 cut(s) 513
FauI CCCGC 1 cut(s) 688
FblI GTMKAC 1 cut(s) 716
Fnu4HI GCNGC 3 cut(s) 99, 303, 510
FokI GGATG 3 cut(s) 50, 172, 402
Fsp4HI GCNGC 3 cut(s) 99, 303, 510
FspBI CTAG 2 cut(s) 345, 422
GlaI GCGC 1 cut(s) 646
GluI GCNGC 3 cut(s) 99, 303, 510
HaeII RGCGCY 1 cut(s) 648
HaeIII GGCC 1 cut(s) 101
HgaI GACGC 1 cut(s) 462
HhaI GCGC 1 cut(s) 647
Hin1II CATG 1 cut(s) 517
Hin6I GCGC 1 cut(s) 645
HinP1I GCGC 1 cut(s) 645
HincII GTYRAC 1 cut(s) 675
HindII GTYRAC 1 cut(s) 675
HindIII AAGCTT 1 cut(s) 684
HinfI GANTC 3 cut(s) 140, 438, 521
HphI GGTGA 5 cut(s) 118, 469, 588, 691, 739
Hpy166II GTNNAC 2 cut(s) 675, 717
Hpy188I TCNGA 2 cut(s) 203, 520
Hpy188III TCNNGA 4 cut(s) 450, 525, 637, 745
Hpy8I GTNNAC 2 cut(s) 675, 717
HpyAV CCTTC 4 cut(s) 199, 584, 622, 751
HpyCH4III ACNGT 2 cut(s) 211, 679
HpyCH4IV ACGT 1 cut(s) 537
HpyCH4V TGCA 3 cut(s) 4, 302, 460
HpyF10VI GCNNNNNNNGC 2 cut(s) 330, 692
HpyF3I CTNAG 2 cut(s) 648, 691
HpySE526I ACGT 1 cut(s) 537
Hsp92II CATG 1 cut(s) 517
HspAI GCGC 1 cut(s) 645
KpnI GGTACC 1 cut(s) 421
Kzo9I GATC 4 cut(s) 263, 271, 425, 633
LmnI GCTCC 2 cut(s) 30, 36
LpnPI CCDG 7 cut(s) 41, 58, 225, 372, 435, 502, 510
Lsp1109I GCAGC 2 cut(s) 314, 521
MaeI CTAG 2 cut(s) 345, 422
MaeII ACGT 1 cut(s) 537
MaeIII GTNAC 1 cut(s) 173
MalI GATC 4 cut(s) 265, 273, 427, 635
MboI GATC 4 cut(s) 263, 271, 425, 633
MboII GAAGA 4 cut(s) 105, 184, 438, 694
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 1 cut(s) 633
MhlI GDGCHC 1 cut(s) 326
MluCI AATT 2 cut(s) 282, 327
MlyI GAGTC 2 cut(s) 134, 530
MmeI TCCRAC 1 cut(s) 573
MnlI CCTC 6 cut(s) 197, 248, 322, 622, 643, 646
MseI TTAA 2 cut(s) 365, 659
MslI CAYNNNNRTG 1 cut(s) 232
MunI CAATTG 1 cut(s) 282
Mva1269I GAATGC 3 cut(s) 22, 223, 460
MwoI GCNNNNNNNGC 2 cut(s) 330, 692
NdeII GATC 4 cut(s) 263, 271, 425, 633
NheI GCTAGC 1 cut(s) 344
NlaIII CATG 1 cut(s) 517
NlaIV GGNNCC 3 cut(s) 26, 41, 419
PctI GAATGC 3 cut(s) 22, 223, 460
PfeI GAWTC 1 cut(s) 438
PkrI GCNGC 3 cut(s) 100, 304, 511
PleI GAGTC 2 cut(s) 134, 529
PpsI GAGTC 2 cut(s) 134, 529
PspEI GGTNACC 1 cut(s) 173
PspN4I GGNNCC 3 cut(s) 26, 41, 419
PsuI RGATCY 1 cut(s) 633
RsaI GTAC 4 cut(s) 208, 410, 419, 536
RsaNI GTAC 4 cut(s) 207, 409, 418, 535
RseI CAYNNNNRTG 1 cut(s) 232
SaqAI TTAA 2 cut(s) 365, 659
SatI GCNGC 3 cut(s) 99, 303, 510
Sau3AI GATC 4 cut(s) 263, 271, 425, 633
SchI GAGTC 2 cut(s) 134, 530
SduI GDGCHC 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 232
SmlI CTYRAG 3 cut(s) 218, 313, 557
SmoI CTYRAG 3 cut(s) 218, 313, 557
Sse9I AATT 2 cut(s) 282, 327
SsiI CCGC 3 cut(s) 98, 415, 695
SspMI CTAG 2 cut(s) 345, 422
StyI CCWWGG 1 cut(s) 421
TaaI ACNGT 2 cut(s) 211, 679
TaiI ACGT 1 cut(s) 540
TaqI TCGA 2 cut(s) 266, 320
TasI AATT 2 cut(s) 282, 327
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 1 cut(s) 438
Tru1I TTAA 2 cut(s) 365, 659
Tru9I TTAA 2 cut(s) 365, 659
TseI GCWGC 2 cut(s) 302, 509
TspDTI ATGAA 4 cut(s) 184, 340, 385, 430
XmaJI CCTAGG 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 716
XspI CTAG 2 cut(s) 345, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.