pycom02g19360

Choline monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010600
Physical Location & Seq
Forward (+)
17206996 .. 17207798
803 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g19360.2

Sequence Viewer

Length: 594 bp
ATGGCTACAGGATACGAGCTACTGCTTGAGTCGGCATCGGAAGTCAACGACTCAAAGTACTTCCTCGGGCTTTCTTCCCTTGGCGCCTTTAGCAAGGCCAAGATGGTCGCGGGCTCATGCCTCAGTTGCTCTTGCTCATTCGGCAAAGTTGATGCAGAGGTGGAAAGCACGGTAGAGAGAGCTATTGCCTTGCTTCGAGTTGTGGCACCCAAAATGCCACCATTTGCAGCAAGGATGTTCTTGTTCTTTGCGTTCGTTGCGGGAACAACTTTATCCTTTCTTGATGCCATGTGTGTTTCATGTGGATTTGAAGACAGAAATGAGAGGCAAAGAAGTCCCATAGGGCATGCCAAATTTCTTTGTTCACAACTTTCCTCTGATTTAGGTCGCGATGACTTGATCTCGAACGAACGATTGAACGATTGCTTCAAGATTTTGTTGATTAAGGGTCTTGGCGACTTGATCTTGAAAACGTTGTTACAAATTTTGAGCTTGCAACAAAGTCTTGAAGGAATCAGCACAAGTGCTTTCTTCAAAGACAGCTTTGACAACGTATTAGTCTTTAAAGATTTGGCAAATGCTCTCCTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 map00260
Pfam Domains
Protein Families
Rieske Ring_hydroxyl_A

Protein Analysis

198

Amino Acids

21.45

Weight (kDa)

5.23

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 83, 205
AccII CGCG 2 cut(s) 110, 390
AciI CCGC 2 cut(s) 110, 260
AclI AACGTT 1 cut(s) 473
AcsI RAATTY 2 cut(s) 353, 483
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 1 cut(s) 59
AgsI TTSAA 6 cut(s) 311, 418, 430, 469, 509, 535
AluBI AGCT 4 cut(s) 19, 182, 492, 543
AluI AGCT 4 cut(s) 19, 182, 492, 543
Ama87I CYCGRG 1 cut(s) 65
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 227
ApoI RAATTY 2 cut(s) 353, 483
AspLEI GCGC 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 65
BanI GGYRCC 2 cut(s) 83, 205
BanII GRGCYC 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 318
BbvI GCAGC 1 cut(s) 239
BccI CCATC 1 cut(s) 97
BciVI GTATCC 1 cut(s) 5
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 87
BfuI GTATCC 1 cut(s) 5
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
BmcAI AGTACT 1 cut(s) 59
BmeT110I CYCGRG 1 cut(s) 65
BmiI GGNNCC 2 cut(s) 85, 207
BmsI GCATC 3 cut(s) 44, 142, 274
BpiI GAAGAC 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 64, 79
BseDI CCNNGG 2 cut(s) 64, 79
BseGI GGATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 136
BseXI GCAGC 1 cut(s) 239
Bsh1236I CGCG 2 cut(s) 110, 390
BshFI GGCC 1 cut(s) 98
BshNI GGYRCC 2 cut(s) 83, 205
BsiHKCI CYCGRG 1 cut(s) 65
BslFI GGGAC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 321
BsnI GGCC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 116
Bsp143I GATC 2 cut(s) 399, 462
Bsp68I TCGCGA 1 cut(s) 390
BspACI CCGC 2 cut(s) 110, 260
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 1 cut(s) 135
BspFNI CGCG 2 cut(s) 110, 390
BspLI GGNNCC 2 cut(s) 85, 207
BspT107I GGYRCC 2 cut(s) 83, 205
BssECI CCNNGG 2 cut(s) 64, 79
BssMI GATC 2 cut(s) 399, 462
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 79
Bst4CI ACNGT 1 cut(s) 172
BstACI GRCGYC 1 cut(s) 84
BstC8I GCNNGC 3 cut(s) 112, 348, 494
BstDEI CTNAG 1 cut(s) 122
BstF5I GGATG 1 cut(s) 240
BstFNI CGCG 2 cut(s) 110, 390
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 2 cut(s) 402, 465
BstMBI GATC 2 cut(s) 399, 462
BstMWI GCNNNNNNNGC 4 cut(s) 90, 126, 141, 257
BstNSI RCATGY 1 cut(s) 350
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 2 cut(s) 110, 390
BstV1I GCAGC 1 cut(s) 239
BstV2I GAAGAC 1 cut(s) 318
BsuI GTATCC 1 cut(s) 5
BsuRI GGCC 1 cut(s) 98
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 1 cut(s) 240
BtuMI TCGCGA 1 cut(s) 390
Cac8I GCNNGC 3 cut(s) 112, 348, 494
CfoI GCGC 1 cut(s) 86
Csp6I GTAC 1 cut(s) 58
CviAII CATG 4 cut(s) 117, 289, 300, 347
CviJI RGCY 8 cut(s) 5, 19, 70, 98, 114, 182, 492, 543
CviKI_1 RGCY 8 cut(s) 5, 19, 70, 98, 114, 182, 492, 543
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 1 cut(s) 122
DinI GGCGCC 1 cut(s) 85
DpnI GATC 2 cut(s) 401, 464
DpnII GATC 2 cut(s) 399, 462
DraI TTTAAA 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 116
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 79
FaeI CATG 4 cut(s) 120, 292, 303, 350
FaiI YATR 5 cut(s) 118, 290, 301, 341, 348
FaqI GGGAC 1 cut(s) 321
FatI CATG 4 cut(s) 116, 288, 299, 346
FauI CCCGC 2 cut(s) 103, 253
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 1 cut(s) 247
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 228
GlaI GCGC 1 cut(s) 85
GluI GCNGC 1 cut(s) 228
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 1 cut(s) 98
HhaI GCGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 4 cut(s) 120, 292, 303, 350
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 3 cut(s) 29, 50, 513
Hpy166II GTNNAC 2 cut(s) 46, 365
Hpy188I TCNGA 2 cut(s) 40, 379
Hpy188III TCNNGA 6 cut(s) 281, 389, 403, 430, 466, 506
Hpy8I GTNNAC 2 cut(s) 46, 365
HpyAV CCTTC 1 cut(s) 503
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 2 cut(s) 473, 552
HpyCH4V TGCA 3 cut(s) 155, 227, 496
HpyF10VI GCNNNNNNNGC 4 cut(s) 90, 126, 141, 257
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 2 cut(s) 473, 552
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 4 cut(s) 120, 292, 303, 350
HspAI GCGC 1 cut(s) 84
KasI GGCGCC 1 cut(s) 83
Kzo9I GATC 2 cut(s) 399, 462
Lsp1109I GCAGC 1 cut(s) 239
LweI GCATC 3 cut(s) 44, 142, 274
MaeII ACGT 2 cut(s) 473, 552
MaeIII GTNAC 1 cut(s) 477
MalI GATC 2 cut(s) 401, 464
MboI GATC 2 cut(s) 399, 462
MboII GAAGA 3 cut(s) 66, 323, 523
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 2 cut(s) 353, 483
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 2 cut(s) 38, 44
MnlI CCTC 5 cut(s) 74, 131, 151, 318, 385
MseI TTAA 2 cut(s) 444, 564
MvnI CGCG 2 cut(s) 110, 390
MwoI GCNNNNNNNGC 4 cut(s) 90, 126, 141, 257
NarI GGCGCC 1 cut(s) 84
NdeII GATC 2 cut(s) 399, 462
NlaIII CATG 4 cut(s) 120, 292, 303, 350
NlaIV GGNNCC 2 cut(s) 85, 207
NruI TCGCGA 1 cut(s) 390
NspI RCATGY 1 cut(s) 350
PaeI GCATGC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 513
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 2 cut(s) 37, 44
PluTI GGCGCC 1 cut(s) 87
PpsI GAGTC 2 cut(s) 37, 44
Psp1406I AACGTT 1 cut(s) 473
PspN4I GGNNCC 2 cut(s) 85, 207
RruI TCGCGA 1 cut(s) 390
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
SaqAI TTAA 2 cut(s) 444, 564
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 2 cut(s) 399, 462
ScaI AGTACT 1 cut(s) 59
SchI GAGTC 2 cut(s) 38, 44
SduI GDGCHC 1 cut(s) 116
SetI ASST 8 cut(s) 21, 162, 184, 388, 476, 494, 545, 555
SfaNI GCATC 3 cut(s) 44, 142, 274
SfcI CTRYAG 1 cut(s) 6
SfoI GGCGCC 1 cut(s) 85
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
SphI GCATGC 1 cut(s) 350
Sse9I AATT 2 cut(s) 353, 483
SsiI CCGC 2 cut(s) 110, 260
SspDI GGCGCC 1 cut(s) 83
StyI CCWWGG 1 cut(s) 79
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 2 cut(s) 476, 555
TaqI TCGA 2 cut(s) 196, 404
TasI AATT 2 cut(s) 353, 483
TatI WGTACW 1 cut(s) 57
TfiI GAWTC 1 cut(s) 513
Tru1I TTAA 2 cut(s) 444, 564
Tru9I TTAA 2 cut(s) 444, 564
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 1 cut(s) 288
XapI RAATTY 2 cut(s) 353, 483
XceI RCATGY 1 cut(s) 350
ZrmI AGTACT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.