pycom04g06970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010600
Physical Location & Seq
Forward (+)
7230938 .. 7231397
460 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g06970.2

Sequence Viewer

Length: 408 bp
ATGATGAGTTCTTTGAGAACGAAGCACTTGCCAACAGGATGGCTCACAACACAATGGTACTTGCAGTACCTAGGATCGTTTGTGCGATTCATTTCTTCAGGACGCTTGCATTCGGGTAGCTCAATCACCTTTTTTTCCAGCAAGTCATCTAACATGGCAGCCATGTCAGAGTCAGGAAAAGGGTACGTTTTCTGTTCCAACTCTCTCAAGGTGCTCTTGTATCTATCTTGGGTGCGAGAAGGCTCACTTGTCTTTATATCCTTCGTCTTGATGGGCGCTGAGGATGTCTTGATGGGGGTAGTGTTGACGGTGAAAGCTTCCTTAGCGGGCTTCTTCCCGGTCTTGTCTACATTTGGGGCGAATACTTTATCCTTATTGAAGTTAGTAATCGGCTCTTTTTTCCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

14.77

Weight (kDa)

9.84

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 347
AciI CCGC 1 cut(s) 326
AclWI GGATC 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 3 cut(s) 59, 68, 185
AgsI TTSAA 1 cut(s) 379
AluBI AGCT 2 cut(s) 120, 317
AluI AGCT 2 cut(s) 120, 317
Alw21I GWGCWC 1 cut(s) 216
AlwI GGATC 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 158
AspA2I CCTAGG 1 cut(s) 70
AspLEI GCGC 1 cut(s) 278
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 2 cut(s) 118, 322
AvrII CCTAGG 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 216
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 1 cut(s) 170
BccI CCATC 3 cut(s) 33, 265, 286
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BcnI CCSGG 1 cut(s) 338
BfaI CTAG 1 cut(s) 71
BfoI RGCGCY 1 cut(s) 279
BisI GCNGC 1 cut(s) 159
BlnI CCTAGG 1 cut(s) 70
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 338
BmrFI CCNGG 1 cut(s) 338
Bpu10I CCTNAGC 2 cut(s) 279, 322
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 338
BsaJI CCNNGG 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 70
BseGI GGATG 2 cut(s) 44, 289
BseMII CTCAG 1 cut(s) 270
BseXI GCAGC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 1 cut(s) 338
BsmI GAATGC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 1 cut(s) 74
BspACI CCGC 1 cut(s) 326
BspCNI CTCAG 1 cut(s) 271
BspPI GGATC 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 1 cut(s) 74
BssT1I CCWWGG 1 cut(s) 70
Bst4CI ACNGT 1 cut(s) 310
BstC8I GCNNGC 2 cut(s) 107, 328
BstDEI CTNAG 2 cut(s) 279, 322
BstF5I GGATG 2 cut(s) 44, 289
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 1 cut(s) 77
BstMBI GATC 1 cut(s) 74
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 170
BstXI CCANNNNNNTGG 1 cut(s) 39
BtsCI GGATG 2 cut(s) 44, 289
Cac8I GCNNGC 2 cut(s) 107, 328
CfoI GCGC 1 cut(s) 278
CseI GACGC 1 cut(s) 111
Csp6I GTAC 3 cut(s) 58, 67, 184
CviAII CATG 2 cut(s) 154, 163
CviJI RGCY 7 cut(s) 43, 120, 161, 243, 317, 330, 393
CviKI_1 RGCY 7 cut(s) 43, 120, 161, 243, 317, 330, 393
CviQI GTAC 3 cut(s) 58, 67, 184
DdeI CTNAG 2 cut(s) 279, 322
DpnI GATC 1 cut(s) 76
DpnII GATC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 70
FaeI CATG 2 cut(s) 157, 166
FaiI YATR 3 cut(s) 155, 164, 257
FalI AAGNNNNNCTT 4 cut(s) 200, 232, 231, 263
FatI CATG 2 cut(s) 153, 162
FauI CCCGC 1 cut(s) 319
FblI GTMKAC 1 cut(s) 347
Fnu4HI GCNGC 1 cut(s) 159
FokI GGATG 2 cut(s) 51, 296
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 71
GlaI GCGC 1 cut(s) 277
GluI GCNGC 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 279
HapII CCGG 1 cut(s) 338
HgaI GACGC 1 cut(s) 111
HhaI GCGC 1 cut(s) 278
Hin1II CATG 2 cut(s) 157, 166
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 2 cut(s) 87, 170
HpaII CCGG 1 cut(s) 338
HphI GGTGA 2 cut(s) 118, 322
Hpy166II GTNNAC 2 cut(s) 306, 348
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 4 cut(s) 99, 174, 268, 289
Hpy8I GTNNAC 2 cut(s) 306, 348
HpyAV CCTTC 2 cut(s) 233, 271
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 2 cut(s) 64, 109
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 2 cut(s) 279, 322
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 2 cut(s) 157, 166
HspAI GCGC 1 cut(s) 276
Kzo9I GATC 1 cut(s) 74
LpnPI CCDG 5 cut(s) 21, 84, 151, 159, 351
Lsp1109I GCAGC 1 cut(s) 170
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 186
MalI GATC 1 cut(s) 76
MboI GATC 1 cut(s) 74
MboII GAAGA 2 cut(s) 87, 325
MhlI GDGCHC 1 cut(s) 216
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 1 cut(s) 274
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 1 cut(s) 338
Mva1269I GAATGC 1 cut(s) 109
MwoI GCNNNNNNNGC 1 cut(s) 323
NciI CCSGG 1 cut(s) 338
NdeII GATC 1 cut(s) 74
NlaIII CATG 2 cut(s) 157, 166
PctI GAATGC 1 cut(s) 109
PfeI GAWTC 1 cut(s) 87
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
RsaI GTAC 3 cut(s) 59, 68, 185
RsaNI GTAC 3 cut(s) 58, 67, 184
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 1 cut(s) 74
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 338
SduI GDGCHC 1 cut(s) 216
SetI ASST 6 cut(s) 72, 122, 131, 189, 213, 319
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
SsiI CCGC 1 cut(s) 326
SspMI CTAG 1 cut(s) 71
StyD4I CCNGG 1 cut(s) 336
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 1 cut(s) 189
TfiI GAWTC 1 cut(s) 87
TseI GCWGC 1 cut(s) 158
TspDTI ATGAA 1 cut(s) 79
XmaJI CCTAGG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 347
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.