MD03G1288500.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002648
Physical Location & Seq
Forward (+)
36720453 .. 36722074
1622 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1288500.v1.1.491

Sequence Viewer

Length: 378 bp
ATGAGTCGTGGGGCGGCAGCGGGGACGAAGGGAGGGAAGAAGAAGGGAGCTTCGTTCACGATAGACTGCGCGAAGCCGGTGGAGGATAAGATCATGGACATCGCCTCACTGGAGAAGTTCCTCCAGGAGCGCATCAAGGTCGGCGGCAAGGCAGGTGCTCTCGGTGACTCCGTCACCGTCTCCCGCGAGAAGAACAAGATCACCGTCACCTCCGACTCCAACTTTTCCAAAAGGTATCTTAAATACTTGACGAAGAAGTACTTGAAGAAGCACAACGTGAGGGATTGGCTTCGCGTGATTGCATCCAACAAGGACCGCAATGTTTATGAATTGAGATACTTCAACATTGCTGAGAACGAGGGTGAGGAGGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22e

Protein Analysis

126

Amino Acids

14.07

Weight (kDa)

9.57

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22e PF01776 15 - 124 1.4e-47 Ribosomal L22e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 143
AccII CGCG 3 cut(s) 71, 186, 294
AciI CCGC 5 cut(s) 14, 20, 144, 184, 316
AdeI CACNNNGTG 1 cut(s) 277
AfaI GTAC 1 cut(s) 260
AgsI TTSAA 2 cut(s) 265, 343
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 1 cut(s) 50
AluI AGCT 1 cut(s) 50
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 1 cut(s) 184
ApeKI GCWGC 1 cut(s) 17
AspLEI GCGC 2 cut(s) 71, 132
AspS9I GGNCC 1 cut(s) 313
AsuHPI GGTGA 5 cut(s) 166, 176, 193, 199, 374
AvaII GGWCC 1 cut(s) 313
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 1 cut(s) 29
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciT130I CCWGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 184
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 3 cut(s) 15, 18, 145
BlsI GCNGC 3 cut(s) 16, 19, 146
BmcAI AGTACT 1 cut(s) 260
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 313
BmgT120I GGNCC 1 cut(s) 313
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 141, 311
BpmI CTGGAG 2 cut(s) 107, 131
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 114
Bse3DI GCAATG 2 cut(s) 325, 345
BseBI CCWGG 1 cut(s) 125
BseGI GGATG 1 cut(s) 302
BseMI GCAATG 2 cut(s) 325, 345
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 29
Bsh1236I CGCG 3 cut(s) 71, 186, 294
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 184
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 2 cut(s) 90, 198
BspACI CCGC 5 cut(s) 14, 20, 144, 184, 316
BspCNI CTCAG 1 cut(s) 343
BspFNI CGCG 3 cut(s) 71, 186, 294
BspMI ACCTGC 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 325, 345
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 76
BssMI GATC 2 cut(s) 90, 198
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 178, 205
BstDEI CTNAG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 302
BstFNI CGCG 3 cut(s) 71, 186, 294
BstHHI GCGC 2 cut(s) 71, 132
BstKTI GATC 2 cut(s) 93, 201
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 2 cut(s) 90, 198
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstUI CGCG 3 cut(s) 71, 186, 294
BstV1I GCAGC 1 cut(s) 29
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 1 cut(s) 302
BtsIMutI CAGTG 1 cut(s) 107
BveI ACCTGC 1 cut(s) 143
CfoI GCGC 2 cut(s) 71, 132
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 313
Csp6I GTAC 1 cut(s) 259
CviAII CATG 1 cut(s) 94
CviJI RGCY 3 cut(s) 50, 76, 289
CviKI_1 RGCY 3 cut(s) 50, 76, 289
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 1 cut(s) 351
DpnI GATC 2 cut(s) 92, 200
DpnII GATC 2 cut(s) 90, 198
DraIII CACNNNGTG 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 1 cut(s) 97
FaiI YATR 2 cut(s) 95, 327
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 1 cut(s) 37
FatI CATG 1 cut(s) 93
FauI CCCGC 2 cut(s) 13, 191
Fnu4HI GCNGC 3 cut(s) 15, 18, 145
FokI GGATG 1 cut(s) 289
Fsp4HI GCNGC 3 cut(s) 15, 18, 145
GlaI GCGC 2 cut(s) 70, 131
GluI GCNGC 3 cut(s) 15, 18, 145
GsuI CTGGAG 2 cut(s) 107, 131
HapII CCGG 1 cut(s) 77
HhaI GCGC 2 cut(s) 71, 132
Hin1II CATG 1 cut(s) 97
Hin6I GCGC 2 cut(s) 69, 130
HinP1I GCGC 2 cut(s) 69, 130
HinfI GANTC 3 cut(s) 4, 167, 215
HpaII CCGG 1 cut(s) 77
HphI GGTGA 5 cut(s) 166, 176, 193, 199, 374
Hpy166II GTNNAC 1 cut(s) 57
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 1 cut(s) 58
Hpy8I GTNNAC 1 cut(s) 57
HpyAV CCTTC 2 cut(s) 22, 37
HpyCH4III ACNGT 2 cut(s) 178, 205
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 1 cut(s) 302
HpyF3I CTNAG 1 cut(s) 351
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 1 cut(s) 97
HspAI GCGC 2 cut(s) 69, 130
Kzo9I GATC 2 cut(s) 90, 198
LmnI GCTCC 2 cut(s) 47, 127
LpnPI CCDG 5 cut(s) 90, 95, 110, 137, 138
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 141, 311
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 3 cut(s) 164, 172, 205
MalI GATC 2 cut(s) 92, 200
MboI GATC 2 cut(s) 90, 198
MboII GAAGA 5 cut(s) 49, 52, 202, 265, 277
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 1 cut(s) 329
MlyI GAGTC 3 cut(s) 13, 161, 209
MmeI TCCRAC 3 cut(s) 237, 243, 330
MnlI CCTC 9 cut(s) 26, 76, 115, 131, 220, 273, 352, 358, 361
MseI TTAA 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 3 cut(s) 71, 186, 294
NdeII GATC 2 cut(s) 90, 198
NlaIII CATG 1 cut(s) 97
NmuCI GTSAC 3 cut(s) 164, 172, 205
PaqCI CACCTGC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PflFI GACNNNGTC 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 123
PkrI GCNGC 3 cut(s) 16, 19, 146
PleI GAGTC 3 cut(s) 12, 161, 209
PpsI GAGTC 3 cut(s) 12, 161, 209
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspPI GGNCC 1 cut(s) 313
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 3 cut(s) 15, 18, 145
Sau3AI GATC 2 cut(s) 90, 198
Sau96I GGNCC 1 cut(s) 313
ScaI AGTACT 1 cut(s) 260
SchI GAGTC 3 cut(s) 13, 161, 209
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 160
SetI ASST 6 cut(s) 52, 141, 157, 212, 236, 279
SfaNI GCATC 2 cut(s) 141, 311
SinI GGWCC 1 cut(s) 313
Sse9I AATT 1 cut(s) 329
SsiI CCGC 5 cut(s) 14, 20, 144, 184, 316
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 178, 205
TaiI ACGT 1 cut(s) 279
TasI AATT 1 cut(s) 329
TatI WGTACW 1 cut(s) 258
TauI GCSGC 2 cut(s) 17, 147
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 3 cut(s) 164, 172, 205
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 3 cut(s) 164, 172, 205
TspDTI ATGAA 1 cut(s) 342
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 1 cut(s) 114
Tth111I GACNNNGTC 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 313
ZrmI AGTACT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.