MD11G1309600.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002648
Physical Location & Seq
Reverse (-)
42304789 .. 42306578
1790 bp
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UTR
Exon/CDS
Intron
MD11G1309600.v1.1.491

Sequence Viewer

Length: 378 bp
ATGAGTCGTGGGGCGGCAGCAGGGACGAAGGGAGGGAAGAAGAAGGGAGCTTCATTCGTGATAGACTGCGCGAAGCCCGTGGAGGATAAGATCATGGACATCGCCTCACTGGAGAAGTTCCTTCAGGAGCGCATCAAGGTCGGCGGCAAGGCCGGTGCTCTCGGCGACTCCGTCACCGTCTCTCGTGAGAAGAACAAGATAACCGTCACCTCCGACTCCAACTTCTCCAAAAGGTATCTTAAGTACTTGACGAAGAAGTACTTGAAGAAGCACAACGTGAGGGATTGGCTTCGCGTGATTGCATCCAACAAGGACCGCAATGTTTATGAATTGAGATACTTCAACATTGCCGAGAACGAGGGTGAGGAGGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22e

Protein Analysis

126

Amino Acids

14.06

Weight (kDa)

9.57

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22e PF01776 15 - 124 2.4e-47 Ribosomal L22e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 71, 294
AciI CCGC 3 cut(s) 14, 144, 316
AcuI CTGAAG 1 cut(s) 107
AdeI CACNNNGTG 1 cut(s) 277
AfaI GTAC 2 cut(s) 245, 260
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 2 cut(s) 265, 343
AluBI AGCT 1 cut(s) 50
AluI AGCT 1 cut(s) 50
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 1 cut(s) 184
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 1 cut(s) 17
AspLEI GCGC 2 cut(s) 71, 132
AspS9I GGNCC 1 cut(s) 313
AsuHPI GGTGA 3 cut(s) 166, 199, 374
AvaII GGWCC 1 cut(s) 313
BauI CACGAG 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 1 cut(s) 29
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BcoDI GTCTC 1 cut(s) 184
BfrI CTTAAG 1 cut(s) 239
BisI GCNGC 3 cut(s) 15, 18, 145
BlsI GCNGC 3 cut(s) 16, 19, 146
BmcAI AGTACT 2 cut(s) 245, 260
Bme18I GGWCC 1 cut(s) 313
BmgT120I GGNCC 1 cut(s) 313
BmsI GCATC 2 cut(s) 141, 311
BpmI CTGGAG 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 78
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 1 cut(s) 114
Bse3DI GCAATG 2 cut(s) 325, 345
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 1 cut(s) 302
BseMI GCAATG 2 cut(s) 325, 345
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 29
Bsh1236I CGCG 2 cut(s) 71, 294
BshFI GGCC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 184
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 1 cut(s) 37
BsnI GGCC 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 1 cut(s) 90
BspACI CCGC 3 cut(s) 14, 144, 316
BspANI GGCC 1 cut(s) 152
BspFNI CGCG 2 cut(s) 71, 294
BspTI CTTAAG 1 cut(s) 239
BsrDI GCAATG 2 cut(s) 325, 345
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 1 cut(s) 90
BssSI CACGAG 1 cut(s) 183
Bst2BI CACGAG 1 cut(s) 183
Bst4CI ACNGT 2 cut(s) 178, 205
BstAFI CTTAAG 1 cut(s) 239
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 1 cut(s) 302
BstFNI CGCG 2 cut(s) 71, 294
BstHHI GCGC 2 cut(s) 71, 132
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 1 cut(s) 90
BstUI CGCG 2 cut(s) 71, 294
BstV1I GCAGC 1 cut(s) 29
BsuRI GGCC 1 cut(s) 152
BtgI CCRYGG 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 1 cut(s) 302
BtsIMutI CAGTG 1 cut(s) 107
CfoI GCGC 2 cut(s) 71, 132
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 313
Csp6I GTAC 2 cut(s) 244, 259
CviAII CATG 1 cut(s) 94
CviJI RGCY 4 cut(s) 50, 76, 152, 289
CviKI_1 RGCY 4 cut(s) 50, 76, 152, 289
CviQI GTAC 2 cut(s) 244, 259
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
DraIII CACNNNGTG 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 107
Esp3I CGTCTC 1 cut(s) 184
FaeI CATG 1 cut(s) 97
FaiI YATR 2 cut(s) 95, 327
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 1 cut(s) 37
FatI CATG 1 cut(s) 93
Fnu4HI GCNGC 3 cut(s) 15, 18, 145
FokI GGATG 1 cut(s) 289
Fsp4HI GCNGC 3 cut(s) 15, 18, 145
GlaI GCGC 2 cut(s) 70, 131
GluI GCNGC 3 cut(s) 15, 18, 145
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 152
HapII CCGG 1 cut(s) 153
HhaI GCGC 2 cut(s) 71, 132
Hin1II CATG 1 cut(s) 97
Hin6I GCGC 2 cut(s) 69, 130
HinP1I GCGC 2 cut(s) 69, 130
HinfI GANTC 3 cut(s) 4, 167, 215
HpaII CCGG 1 cut(s) 153
HphI GGTGA 3 cut(s) 166, 199, 374
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 3 cut(s) 58, 125, 185
HpyAV CCTTC 3 cut(s) 22, 37, 131
HpyCH4III ACNGT 2 cut(s) 178, 205
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 1 cut(s) 302
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 1 cut(s) 97
HspAI GCGC 2 cut(s) 69, 130
Kzo9I GATC 1 cut(s) 90
LmnI GCTCC 2 cut(s) 47, 127
LpnPI CCDG 4 cut(s) 6, 95, 110, 166
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 141, 311
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 172, 205
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 5 cut(s) 49, 52, 202, 265, 277
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 1 cut(s) 329
MlyI GAGTC 3 cut(s) 13, 161, 209
MmeI TCCRAC 3 cut(s) 237, 243, 330
MnlI CCTC 8 cut(s) 26, 76, 115, 220, 273, 352, 358, 361
MseI TTAA 1 cut(s) 240
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 1 cut(s) 153
MvnI CGCG 2 cut(s) 71, 294
NdeII GATC 1 cut(s) 90
NlaIII CATG 1 cut(s) 97
NmeAIII GCCGAG 2 cut(s) 141, 376
NmuCI GTSAC 2 cut(s) 172, 205
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PflFI GACNNNGTC 1 cut(s) 170
PkrI GCNGC 3 cut(s) 16, 19, 146
PleI GAGTC 3 cut(s) 12, 161, 209
PpsI GAGTC 3 cut(s) 12, 161, 209
PspPI GGNCC 1 cut(s) 313
PsyI GACNNNGTC 1 cut(s) 170
RsaI GTAC 2 cut(s) 245, 260
RsaNI GTAC 2 cut(s) 244, 259
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 3 cut(s) 15, 18, 145
Sau3AI GATC 1 cut(s) 90
Sau96I GGNCC 1 cut(s) 313
ScaI AGTACT 2 cut(s) 245, 260
SchI GAGTC 3 cut(s) 13, 161, 209
SduI GDGCHC 1 cut(s) 160
SetI ASST 5 cut(s) 52, 141, 212, 236, 279
SfaNI GCATC 2 cut(s) 141, 311
SinI GGWCC 1 cut(s) 313
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 1 cut(s) 329
SsiI CCGC 3 cut(s) 14, 144, 316
TaaI ACNGT 2 cut(s) 178, 205
TaiI ACGT 1 cut(s) 279
TasI AATT 1 cut(s) 329
TatI WGTACW 2 cut(s) 243, 258
TauI GCSGC 2 cut(s) 17, 147
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 2 cut(s) 172, 205
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 2 cut(s) 172, 205
TspDTI ATGAA 2 cut(s) 42, 342
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 1 cut(s) 114
Tth111I GACNNNGTC 1 cut(s) 170
Vha464I CTTAAG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 313
ZrmI AGTACT 2 cut(s) 245, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.