Prupe.8G261700_v2.0.a1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0002648
Physical Location & Seq
Forward (+)
22084706 .. 22086196
1491 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G261700.1

Sequence Viewer

Length: 375 bp
ATGAGTCGTGGGGCGGCAGCTGGGACGAAGGGGAAGAAGAAGGGGGCTTCCTTTGTGATAGACTGCGCGAAGCCAGTGGAGGATAAGATCATGGACATCGCCTCACTGGAAAAGTTCCTCCAGGAGCGCATCAAGGTCGGCGGCAAGGCCGGTGCTCTCGGTGACGCCGTCACTGTCTCACGTGAGAAGAACAAGATCACCGTCACCTCTGACTCCAACTTCTCTAAGAGGTATCTTAAATACTTGACCAAGAAGTACTTGAAGAAGCACAATGTGAGGGATTGGCTTCGCGTAATTGCTTCCAACAAGGACCGCGGTGTTTATGAGTTGAGATACTTCAACATTGCTGAGAACGAGGGAGAGGAGGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22e

Protein Analysis

125

Amino Acids

13.93

Weight (kDa)

9.57

Isoelectric Point (pI)

30.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 68, 291, 315
AciI CCGC 4 cut(s) 14, 141, 313, 315
AcvI CACGTG 1 cut(s) 182
AcyI GRCGYC 1 cut(s) 165
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 1 cut(s) 257
AgsI TTSAA 2 cut(s) 262, 340
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 1 cut(s) 20
AluI AGCT 1 cut(s) 20
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 1 cut(s) 181
AoxI GGCC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 17
AspLEI GCGC 2 cut(s) 68, 129
AspS9I GGNCC 1 cut(s) 310
AsuHPI GGTGA 3 cut(s) 173, 190, 196
AvaII GGWCC 1 cut(s) 310
BbrPI CACGTG 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 157
BbvI GCAGC 1 cut(s) 29
BceAI ACGGC 1 cut(s) 152
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 181
BisI GCNGC 3 cut(s) 15, 18, 142
BlsI GCNGC 3 cut(s) 16, 19, 143
BmcAI AGTACT 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 310
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 1 cut(s) 138
BpmI CTGGAG 1 cut(s) 104
BsaAI YACGTR 1 cut(s) 182
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 313
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 2 cut(s) 74, 111
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 313
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 2 cut(s) 74, 111
BseXI GCAGC 1 cut(s) 29
BseYI CCCAGC 1 cut(s) 20
Bsh1236I CGCG 3 cut(s) 68, 291, 315
BshFI GGCC 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 157
BsiSI CCGG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 181
BsmFI GGGAC 1 cut(s) 37
BsnI GGCC 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 157
Bsp143I GATC 2 cut(s) 87, 195
BspACI CCGC 4 cut(s) 14, 141, 313, 315
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 1 cut(s) 340
BspFNI CGCG 3 cut(s) 68, 291, 315
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 2 cut(s) 74, 111
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 2 cut(s) 87, 195
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 2 cut(s) 175, 202
BstACI GRCGYC 1 cut(s) 165
BstBAI YACGTR 1 cut(s) 182
BstDEI CTNAG 2 cut(s) 225, 348
BstDSI CCRYGG 1 cut(s) 313
BstFNI CGCG 3 cut(s) 68, 291, 315
BstHHI GCGC 2 cut(s) 68, 129
BstKTI GATC 2 cut(s) 90, 198
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 2 cut(s) 87, 195
BstNI CCWGG 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 120
BstUI CGCG 3 cut(s) 68, 291, 315
BstV1I GCAGC 1 cut(s) 29
BsuRI GGCC 1 cut(s) 149
BtgI CCRYGG 1 cut(s) 313
BtgZI GCGATG 1 cut(s) 82
BtsIMutI CAGTG 3 cut(s) 81, 104, 171
CfoI GCGC 2 cut(s) 68, 129
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 310
Cfr42I CCGCGG 1 cut(s) 316
CseI GACGC 1 cut(s) 173
Csp6I GTAC 1 cut(s) 256
CviAII CATG 1 cut(s) 91
CviJI RGCY 5 cut(s) 20, 47, 73, 149, 286
CviKI_1 RGCY 5 cut(s) 20, 47, 73, 149, 286
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 2 cut(s) 225, 348
DpnI GATC 2 cut(s) 89, 197
DpnII GATC 2 cut(s) 87, 195
DraIII CACNNNGTG 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 310
Eco72I CACGTG 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 120
FaeI CATG 1 cut(s) 94
FaiI YATR 2 cut(s) 92, 324
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 1 cut(s) 37
FatI CATG 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 15, 18, 142
Fsp4HI GCNGC 3 cut(s) 15, 18, 142
GlaI GCGC 2 cut(s) 67, 128
GluI GCNGC 3 cut(s) 15, 18, 142
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 1 cut(s) 104
HaeIII GGCC 1 cut(s) 149
HapII CCGG 1 cut(s) 150
HgaI GACGC 1 cut(s) 173
HhaI GCGC 2 cut(s) 68, 129
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 2 cut(s) 66, 127
HinP1I GCGC 2 cut(s) 66, 127
HinfI GANTC 2 cut(s) 4, 212
HpaII CCGG 1 cut(s) 150
HphI GGTGA 3 cut(s) 173, 190, 196
Hpy188I TCNGA 1 cut(s) 211
HpyAV CCTTC 2 cut(s) 22, 34
HpyCH4III ACNGT 2 cut(s) 175, 202
HpyCH4IV ACGT 1 cut(s) 181
HpyF3I CTNAG 2 cut(s) 225, 348
HpySE526I ACGT 1 cut(s) 181
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 2 cut(s) 66, 127
KspI CCGCGG 1 cut(s) 316
Kzo9I GATC 2 cut(s) 87, 195
LmnI GCTCC 1 cut(s) 124
LpnPI CCDG 6 cut(s) 6, 87, 92, 107, 134, 163
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 1 cut(s) 138
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 3 cut(s) 161, 169, 202
MalI GATC 2 cut(s) 89, 197
MboI GATC 2 cut(s) 87, 195
MboII GAAGA 4 cut(s) 46, 49, 199, 274
MhlI GDGCHC 1 cut(s) 157
MluCI AATT 1 cut(s) 294
MlyI GAGTC 2 cut(s) 13, 206
MmeI TCCRAC 2 cut(s) 240, 327
MnlI CCTC 9 cut(s) 73, 112, 128, 217, 222, 270, 349, 355, 358
MseI TTAA 1 cut(s) 237
MspA1I CMGCKG 2 cut(s) 20, 315
MspI CCGG 1 cut(s) 150
MspR9I CCNGG 1 cut(s) 122
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 3 cut(s) 68, 291, 315
NdeII GATC 2 cut(s) 87, 195
NlaIII CATG 1 cut(s) 94
NmuCI GTSAC 3 cut(s) 161, 169, 202
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PflFI GACNNNGTC 1 cut(s) 167
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 3 cut(s) 16, 19, 143
PleI GAGTC 2 cut(s) 12, 206
PmaCI CACGTG 1 cut(s) 182
PmlI CACGTG 1 cut(s) 182
PpsI GAGTC 2 cut(s) 12, 206
Ppu21I YACGTR 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 120
PspCI CACGTG 1 cut(s) 182
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 1 cut(s) 120
PspPI GGNCC 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 167
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SacII CCGCGG 1 cut(s) 316
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 3 cut(s) 15, 18, 142
Sau3AI GATC 2 cut(s) 87, 195
Sau96I GGNCC 1 cut(s) 310
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 2 cut(s) 13, 206
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 1 cut(s) 157
SetI ASST 5 cut(s) 22, 138, 184, 209, 233
SfaNI GCATC 1 cut(s) 138
Sfr303I CCGCGG 1 cut(s) 316
SgrBI CCGCGG 1 cut(s) 316
SinI GGWCC 1 cut(s) 310
Sse9I AATT 1 cut(s) 294
SsiI CCGC 4 cut(s) 14, 141, 313, 315
StyD4I CCNGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 175, 202
TaiI ACGT 1 cut(s) 184
TasI AATT 1 cut(s) 294
TatI WGTACW 1 cut(s) 255
TauI GCSGC 2 cut(s) 17, 144
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 3 cut(s) 81, 111, 178
TseFI GTSAC 3 cut(s) 161, 169, 202
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 3 cut(s) 161, 169, 202
TspRI CASTG 3 cut(s) 81, 111, 178
Tth111I GACNNNGTC 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 310
ZrmI AGTACT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.