Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004176

Physical Location & Seq

Genomic Coordinates Reverse (-)

877245 .. 884741

Gene Structure 7497 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1007800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3672 bp

ATGCATAAGCGATCTGGTGGGACGGCCCCGGAGCCGGTGCGGACGAGCGGAAGGCTGAAGGGGCGGACGACGCCGTACGGGCGGGGCATATACTACTACACCACAAGCACTTCCAGGAAGATCATCCAAAAGCGGAGAAACCGGAAGAACAAGACCAAGACCCGAACCGCCGCTTCCCATATCGCTAAGATAATGCGCCATGGCGGCACTCCTCGTCCCCAAAGATCCACCACTCCTCTCCCCTCCAGCAACCTCAATGCAGATGCATCCAACCTCCGGCGCTCCACAAGAAAGAGAACGATGTCAGTAAGGAATCGGGGCGACTACATAACAGACGACAGTTCTGGAAGTGAGCAGGATGAAGATATAATGAAAGTCAGGAGTAAATCTATCAAGAATAAGGTTGATAATAGTTCGTTTAAAGATGAGTTGCCATCTCCCAAGCACAAAAAGATGGTGGAGACTAGGCATACACCTCGTCGCGAAGGATTGCGCCCTCGTCGGTTCAATTCCGCAAGAGAACAATTGCATTTGCAGTTTGATGATGAGCCGGGTACTTCAGATGAGAAGATTGATCAAGATGAAACTGAAAATGGGAATGACATTGAATACAATGATGCAGATGATGCTCAAAATGAAGCTGACGGTGAGGATGTTGGTGATGGTGAGGGTGATGATGATGGTGATGGTGATGGCGATGGCGATGGTGATGAAGACGGTGATGATGAGGAGGGTGATGAAGAACAAGATGGACGAAGACGATATGATCTTCGAAATCGTGCAGATGTCCGTAAGCTGAGACCAAGATCTCCTCGTAGAGTATTGCGTCAAGGAATGGGGACAAAGGTCGGTAGGGATGTGAGAAAGGGTGGATCACGAGTTCATAAGCGCCATCGTATGACAAGGACAGATGATTCTGATGACTCCCTTCTTGTCGACGAGCTAGACCAAGGACCAGCAATTCCCTGGGCCCGAGGTGGGAGCAGATCTGGACCACCTTGGCTCTTTGGGGGATTAGACATGCATGGAACAACAGCATGGGGATTGAATGTTGCTGCTTCAGGTTGGGGTCATCAGGGTGATGCTTTTGCCACCTTAACATCTGGAATTCAAACTGCTGGGCCAAGTTCAAAGGGAGGGGCAGACATACAACCTTTGCAGGTTGATGATAGTGTGAGTTTTGATGATATAGGTGGGCTTTCTGAATATATTGATGCTTTGAAGGAGATGGTTTTCTTTCCTCTGTTATATCCAGATTTCTTTGCAAGTTATCATATCACCCCACCAAGGGGGGTTTTGTTATGTGGTCCTCCTGGGACTGGGAAAACATTAATTGCCAGAGCGTTAGCCTGTGCTGCATCAAAGGCTGGCCAGAAGGTCAGCTTTTACATGCGGAAGGGTGCAGATGTGCTAAGTAAATGGGTGGGTGAGGCTGAAAGACAATTAAAACTACTTTTTGAGGAAGCACAAAGAAATCAACCTTCGATCATATTCTTTGATGAAATAGATGGACTTGCTCCTGTAAGGTCCAGCAAACAAGAGCAAATTCACAATTCTATTGTCTCAACTTTGCTTGCTTTGATGGATGGTCTTGATTCCCGTGGCCAAGTCGTTTTGATTGGAGCAACCAACCGGATCGATGCAATTGATGGAGCTCTGCGGCGCCCTGGTAGGTTTGACCGTGAATTCAACTTTCCTTTGCCTGGCTGTGAAGCACGTGCTGAAATTTTAGACATTCATACTCGGAAATGGAGGAGTCCTCCTTCAAGGGAGCTAAAATTGGAACTTGCAGCTAGTTGTGTTGGATATTGTGGTGCTGATTTAAAGGCTCTTTGCACTGAAGCTGCCATTCGTGCTTTCCGAGAAAAATATCCTCAGGTTTACACAAGTGATGATAAGTTTGTTATCGATGTTGATTCAGTAAACGTTGAGAAGTATCACTTTGTTGAAGCCATGTCAACAATTACTCCAGCTGCTCACAGAGGTGCTGTTGTGCACTCCAGGCCATTGTCTTCAGTAGTAGCACCATGCCTGCAAATGCATCTCCAAAAAGCCATGAAGTATATATCCGATATTTTCCCACCCCTTGCAGTTTCATCACAGTTGACCAAGCTTTCCATGCTGTCCTCTGGCTCTGCAATTCCTCTTGTCTACAGGCCTCGGCTTCTGCTATGTGGTGGTGAAGGTTCTGGCCTGGATCATCTTGGGCCTGCTGTTTTACATGAACTTGAGAAATTCCCTGTTCATTCTTTGGGACTTCCATCTCTTCTATCTGATCCCAGTGCAAAGACACCAGAGGAGGCACTGGTACATATATTTGGTGAGGCAAGGAGAACAACTCCATCAATTCTCTATTTGCCTCAGCTCAATATTTGGTGGGAAACAGCACATGAGCAGCTCCGAGCAGTTTTGCTGACGTTGTTAGAAGAATTTCCATCTGACTTGCCAATATTACTTCTTGCAACATCATCAGTGCCACCTGCTGAAGTTGACACTACAGCTTCTTCAATATTTTCTGAACGTTCTATCTATCAGTTGGGCAAACCATCTACTGAAGACAGATATTTGTTTTTTGACCGTCTCATCGAAGCTGCTTTATCAGTGTTGTTAGAGGAGATAACAAAAAAATCCCAGGAATCAGTATCTGTTCCAGAACTTCCCAAGGCCCCAAAAGTGGCAAGTGGTCCGAAGGTCTCGGAGTTAAAAGCCAAGGTAGAAGCTGAGCAGCATGCTCTTCGCCGATTGCGGATGTGCCTCAGAGATGTTTGCAACCGACTTTTATATGATAAGAGGTTTAATGCTTTCCATTATCCTGTCTCGGATGAGGATGCTCCAAACTATCGCTCAATTATTCAGAACCCTATGGATGTGGCTAAGCTGCTGCAATGCGTGGACTCTGGGCAGTACATTACATGTTCTGCATTCCTGCAAGACGTTGATCTTATTGTTTCCAACGCAAAGGCTTATAATGGAGATGATTACAATGGTGCTAGGATTGTCAGCAGAGCTTTTGAGCTTCGAGATGCAGTACATGGGATGCTGTCACAAATGGACCCAGCACTTCTTGCATATTGTGACAAGATTGCAGCTCAAGGTGGTCCAGAACATATTCCCGATGATTTAGGGGTATCCACTTTCCCCTCTGAACCTGTTGTACAGCTGGGAACTGTCACCAGGGCAAGTGCTCGACTTCGTAATGTCCAGGTGGATGTAAATCTGGATCCGAGCCATGAGTCTCAGAAAAGGCCGAAGAAGCATGTTGAGCCTGCACATGCAGATTCGACTCCAGAAGACAAGTCAGAGCATCAAGATTCAACACCGTTGAAGACATCTCAGGAACCGGAGACGAATGACTCAAATCCTGAAGTCCCAGAGTCCTCTTCTGCTGGTGGCAATCAGCTTGAGACTCCTGTAGAAGTTTCAGGCCATGCCGATATGATTGGATCCCAAGACACCGTCATGTCGGATGTCGAGGTCACAAACCAAATAGAGTCAGTCAGGCGGCTTCTTGTTGAAAGGACTGCAAACTATGACATTCCGCAACTCGAAAGGCTGTACACAAGAATTATGAAGGCCATTTTTGACATCAAGCACAAGGGTGACATGGATGGCACCAAGCCTTCAATTTTGAGGTACTTATTGAAATACGCAGAGGATGAAGCAAATTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003682 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010639 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031935 GO:0031936 GO:0033043 GO:0033044 GO:0034641 GO:0035510 GO:0040029 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045815 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0080090 GO:0080111 GO:0090304 GO:1901360 GO:1902275 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:2000112 GO:2001141 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Bromodomain

Protein Analysis

1224

Amino Acids

135.37

Weight (kDa)

5.88

Isoelectric Point (pI)

52.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IstB_IS21	PF01695	430 - 514	3.7e-07	IstB-like ATP binding protein
AAA	PF00004	432 - 567	1.4e-38	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	595 - 631	1.8e-10	AAA+ lid domain
Bromodomain	PF00439	922 - 993	4.8e-13	Bromodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05910
fragaria_vesca	FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720
malus_domestica	MD04G1007800.v1.1
prunus_persica	Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1
pyrus_communis	pycom04g03460
rosa_chinensis	RchiOBHm_Chr3g0496841 RchiOBHm_Chr4g0406861
rosa_laevigata	RLG00000008748 RLG00000022493
rosa_multiflora	Rmu_sc0000600.1_g000031 Rmu_sc0001438.1_g000025
rosa_roxburghii	Rroxscaffold_5G00351650 Rroxscaffold_6G00388620
rosa_rugosa	Rorug03G0288300 Rorug04G0075300
rosa_samantha	Rh3AG332000 Rh3BG368600 Rh3CG364900 Rh3DG368000 Rh4AG141500 Rh4BG138300 Rh4CG148200 Rh4DG135400
rosa_wichuraiana	Rw3G029090 Rw4G011520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2967
AarI	CACCTGC	1 cut(s)	2488
Acc36I	ACCTGC	2 cut(s)	1150, 2488
AccB1I	GGYRCC	2 cut(s)	1662, 3611
AccBSI	CCGCTC	1 cut(s)	48
AccI	GTMKAC	2 cut(s)	936, 2151
AccII	CGCG	1 cut(s)	483
AclI	AACGTT	2 cut(s)	1926, 2521
AclWI	GGATC	9 cut(s)	219, 880, 1643, 2205, 2270, 3215, 3228, 3438, 3451
AcoI	YGGCCR	2 cut(s)	1369, 1603
AcsI	RAATTY	7 cut(s)	1107, 1545, 1685, 1725, 2234, 2429, 3664
AcuI	CTGAAG	8 cut(s)	77, 543, 1044, 1860, 1998, 2505, 2574, 3384
AcvI	CACGTG	1 cut(s)	1718
AcyI	GRCGYC	2 cut(s)	71, 1663
AfaI	GTAC	8 cut(s)	77, 556, 2310, 2906, 3030, 3156, 3557, 3635
AfiI	CCNNNNNNNGG	9 cut(s)	34, 276, 978, 1287, 1288, 1289, 1319, 1703, 2674
AflIII	ACRYGT	1 cut(s)	2912
AleI	CACNNNNGTG	2 cut(s)	1983, 3597
AloI	GAACNNNNNNTCC	2 cut(s)	864, 896
Alw21I	GWGCWC	3 cut(s)	1657, 1998, 3187
Alw26I	GTCTC	9 cut(s)	455, 793, 1567, 2585, 2698, 2821, 3240, 3338, 3398
Alw44I	GTGCAC	1 cut(s)	1994
AlwI	GGATC	9 cut(s)	219, 880, 1643, 2205, 2270, 3215, 3228, 3438, 3451
AlwNI	CAGNNNCTG	1 cut(s)	2645
Ama87I	CYCGRG	1 cut(s)	972
ApaI	GGGCCC	1 cut(s)	973
ApaLI	GTGCAC	1 cut(s)	1994
ApoI	RAATTY	7 cut(s)	1107, 1545, 1685, 1725, 2234, 2429, 3664
AseI	ATTAAT	1 cut(s)	1331
Asp700I	GAANNNNTTC	2 cut(s)	2430, 3108
AspLEI	GCGC	5 cut(s)	198, 282, 495, 891, 1665
AsuC2I	CCSGG	2 cut(s)	29, 552
AsuII	TTCGAA	1 cut(s)	772
AvaI	CYCGRG	1 cut(s)	972
AvaII	GGWCC	7 cut(s)	953, 992, 1307, 1527, 2684, 3052, 3098
AxyI	CCTNAGG	1 cut(s)	1875
BaeGI	GKGCMC	2 cut(s)	973, 1998
BalI	TGGCCA	2 cut(s)	1371, 1605
BamHI	GGATCC	2 cut(s)	3220, 3443
BanI	GGYRCC	2 cut(s)	1662, 3611
BanII	GRGCYC	2 cut(s)	973, 1657
BauI	CACGAG	1 cut(s)	876
BbrPI	CACGTG	1 cut(s)	1718
BbsI	GAAGAC	6 cut(s)	720, 763, 2004, 2562, 3297, 3332
Bbv12I	GWGCWC	3 cut(s)	1657, 1998, 3187
BbvCI	CCTCAGC	1 cut(s)	2361
BceAI	ACGGC	2 cut(s)	39, 58
BcgI	CGANNNNNNTGC	4 cut(s)	458, 492, 2717, 2751
BciVI	GTATCC	1 cut(s)	3139
BclI	TGATCA	1 cut(s)	574
BcnI	CCSGG	2 cut(s)	29, 552
BcoDI	GTCTC	9 cut(s)	455, 793, 1567, 2585, 2698, 2821, 3240, 3338, 3398
BfaI	CTAG	4 cut(s)	465, 944, 1794, 2991
BfmI	CTRYAG	3 cut(s)	2152, 2496, 3411
BfoI	RGCGCY	3 cut(s)	283, 892, 1666
BfuAI	ACCTGC	2 cut(s)	1150, 2488
BfuI	GTATCC	1 cut(s)	3139
BglI	GCCNNNNNGGC	2 cut(s)	79, 204
BglII	AGATCT	2 cut(s)	806, 986
BlpI	GCTNAGC	2 cut(s)	2721, 2874
Bme18I	GGWCC	7 cut(s)	953, 992, 1307, 1527, 2684, 3052, 3098
BmeT110I	CYCGRG	1 cut(s)	972
BmrI	ACTGGG	2 cut(s)	1329, 2274
BmuI	ACTGGG	2 cut(s)	1329, 2274
BoxI	GACNNNNGTC	1 cut(s)	845
BpiI	GAAGAC	6 cut(s)	720, 763, 2004, 2562, 3297, 3332
BplI	GAGNNNNNCTC	4 cut(s)	1744, 1776, 2323, 2355
BpmI	CTGGAG	4 cut(s)	229, 1953, 1984, 3270
Bpu10I	CCTNAGC	1 cut(s)	2361
Bpu1102I	GCTNAGC	2 cut(s)	2721, 2874
Bpu14I	TTCGAA	1 cut(s)	772
BpuEI	CTTGAG	3 cut(s)	2249, 3075, 3422
BpuMI	CCSGG	2 cut(s)	29, 552
Bsa29I	ATCGAT	2 cut(s)	1638, 1908
BsaAI	YACGTR	1 cut(s)	1718
BsaBI	GATNNNNATC	1 cut(s)	3213
BsaHI	GRCGYC	2 cut(s)	71, 1663
BsaI	GGTCTC	2 cut(s)	793, 2698
BsaWI	WCCGGW	3 cut(s)	141, 1632, 3340
BsaXI	ACNNNNNCTCC	2 cut(s)	1951, 1981
Bsc4I	CCNNNNNNNGG	9 cut(s)	34, 276, 978, 1287, 1288, 1289, 1319, 1703, 2674
Bse118I	RCCGGY	1 cut(s)	34
Bse1I	ACTGG	3 cut(s)	1324, 2280, 2310
Bse21I	CCTNAGG	1 cut(s)	1875
Bse3DI	GCAATG	1 cut(s)	2891
Bse8I	GATNNNNATC	1 cut(s)	3213
BseCI	ATCGAT	2 cut(s)	1638, 1908
BseJI	GATNNNNATC	1 cut(s)	3213
BseLI	CCNNNNNNNGG	9 cut(s)	34, 276, 978, 1287, 1288, 1289, 1319, 1703, 2674
BseMI	GCAATG	1 cut(s)	2891
BseMII	CTCAG	7 cut(s)	788, 1889, 2375, 2712, 2770, 3251, 3347
BseNI	ACTGG	3 cut(s)	1324, 2280, 2310
BseRI	GAGGAG	7 cut(s)	201, 225, 743, 801, 1768, 2312, 2627
BseSI	GKGCMC	2 cut(s)	973, 1998
BseYI	CCCAGC	3 cut(s)	1118, 3055, 3160
BsgI	GTGCAG	3 cut(s)	801, 1422, 3252
Bsh1236I	CGCG	1 cut(s)	483
BshNI	GGYRCC	2 cut(s)	1662, 3611
BshVI	ATCGAT	2 cut(s)	1638, 1908
BsiHKAI	GWGCWC	3 cut(s)	1657, 1998, 3187
BsiHKCI	CYCGRG	1 cut(s)	972
BsiSI	CCGG	7 cut(s)	29, 35, 142, 277, 551, 1633, 3341
BsiWI	CGTACG	1 cut(s)	75
BslFI	GGGAC	6 cut(s)	34, 201, 853, 1330, 2268, 3353
BslI	CCNNNNNNNGG	9 cut(s)	34, 276, 978, 1287, 1288, 1289, 1319, 1703, 2674
BsmAI	GTCTC	9 cut(s)	455, 793, 1567, 2585, 2698, 2821, 3240, 3338, 3398
BsmBI	CGTCTC	2 cut(s)	2585, 3338
BsmFI	GGGAC	6 cut(s)	34, 201, 853, 1330, 2268, 3353
BsmI	GAATGC	1 cut(s)	2921
Bso31I	GGTCTC	2 cut(s)	793, 2698
BsoBI	CYCGRG	1 cut(s)	972
Bsp119I	TTCGAA	1 cut(s)	772
Bsp120I	GGGCCC	1 cut(s)	969
Bsp1286I	GDGCHC	4 cut(s)	973, 1657, 1998, 3187
Bsp1407I	TGTACA	2 cut(s)	3154, 3555
Bsp1720I	GCTNAGC	2 cut(s)	2721, 2874
Bsp19I	CCATGG	1 cut(s)	199
Bsp68I	TCGCGA	1 cut(s)	483
BspCNI	CTCAG	7 cut(s)	789, 1888, 2374, 2713, 2769, 3250, 3346
BspDI	ATCGAT	2 cut(s)	1638, 1908
BspFNI	CGCG	1 cut(s)	483
BspMI	ACCTGC	2 cut(s)	1150, 2488
BspPI	GGATC	9 cut(s)	219, 880, 1643, 2205, 2270, 3215, 3228, 3438, 3451
BspQI	GCTCTTC	1 cut(s)	2739
BspT104I	TTCGAA	1 cut(s)	772
BspT107I	GGYRCC	2 cut(s)	1662, 3611
BspTNI	GGTCTC	2 cut(s)	793, 2698
BsrBI	CCGCTC	1 cut(s)	48
BsrDI	GCAATG	1 cut(s)	2891
BsrFI	RCCGGY	1 cut(s)	34
BsrGI	TGTACA	2 cut(s)	3154, 3555
BsrI	ACTGG	3 cut(s)	1324, 2280, 2310
BssAI	RCCGGY	1 cut(s)	34
BssNI	GRCGYC	2 cut(s)	71, 1663
BssSI	CACGAG	1 cut(s)	876
BssT1I	CCWWGG	6 cut(s)	199, 949, 998, 1286, 2661, 2709
Bst2BI	CACGAG	1 cut(s)	876
Bst4CI	ACNGT	9 cut(s)	341, 647, 719, 1682, 2103, 2579, 3169, 3321, 3457
Bst6I	CTCTTC	3 cut(s)	2271, 2739, 3385
BstACI	GRCGYC	2 cut(s)	71, 1663
BstAPI	GCANNNNNTGC	2 cut(s)	626, 3065
BstAUI	TGTACA	2 cut(s)	3154, 3555
BstBAI	YACGTR	1 cut(s)	1718
BstBI	TTCGAA	1 cut(s)	772
BstC8I	GCNNGC	6 cut(s)	1369, 1575, 2033, 2211, 2730, 3267
BstDSI	CCRYGG	2 cut(s)	199, 1600
BstFNI	CGCG	1 cut(s)	483
BstH2I	RGCGCY	3 cut(s)	283, 892, 1666
BstHHI	GCGC	5 cut(s)	198, 282, 495, 891, 1665
BstMAI	GTCTC	9 cut(s)	455, 793, 1567, 2585, 2698, 2821, 3240, 3338, 3398
BstNSI	RCATGY	6 cut(s)	1024, 1393, 2732, 2916, 3260, 3275
BstPAI	GACNNNNGTC	1 cut(s)	845
BstSFI	CTRYAG	3 cut(s)	2152, 2496, 3411
BstSLI	GKGCMC	2 cut(s)	973, 1998
BstUI	CGCG	1 cut(s)	483
BstV2I	GAAGAC	6 cut(s)	720, 763, 2004, 2562, 3297, 3332
BstX2I	RGATCY	5 cut(s)	224, 806, 986, 3220, 3443
BstYI	RGATCY	5 cut(s)	224, 806, 986, 3220, 3443
Bsu15I	ATCGAT	2 cut(s)	1638, 1908
Bsu36I	CCTNAGG	1 cut(s)	1875
BsuI	GTATCC	1 cut(s)	3139
BsuTUI	ATCGAT	2 cut(s)	1638, 1908
BtgI	CCRYGG	2 cut(s)	199, 1600
BtgZI	GCGATG	2 cut(s)	711, 717
BtsIMutI	CAGTG	5 cut(s)	1836, 2287, 2303, 2478, 2607
BtuMI	TCGCGA	1 cut(s)	483
BveI	ACCTGC	2 cut(s)	1150, 2488
Cac8I	GCNNGC	6 cut(s)	1369, 1575, 2033, 2211, 2730, 3267
CaiI	CAGNNNCTG	1 cut(s)	2645
CfoI	GCGC	5 cut(s)	198, 282, 495, 891, 1665
Cfr10I	RCCGGY	1 cut(s)	34
ClaI	ATCGAT	2 cut(s)	1638, 1908
CseI	GACGC	2 cut(s)	79, 815
Csp6I	GTAC	8 cut(s)	76, 555, 2309, 2905, 3029, 3155, 3556, 3634
CviQI	GTAC	8 cut(s)	76, 555, 2309, 2905, 3029, 3155, 3556, 3634
DinI	GGCGCC	1 cut(s)	1664
DraI	TTTAAA	2 cut(s)	421, 1824
EaeI	YGGCCR	2 cut(s)	1369, 1603
Eam1104I	CTCTTC	3 cut(s)	2271, 2739, 3385
EarI	CTCTTC	3 cut(s)	2271, 2739, 3385
EciI	GGCGGA	1 cut(s)	79
Ecl136II	GAGCTC	1 cut(s)	1655
Eco130I	CCWWGG	6 cut(s)	199, 949, 998, 1286, 2661, 2709
Eco147I	AGGCCT	1 cut(s)	2158
Eco24I	GRGCYC	2 cut(s)	973, 1657
Eco31I	GGTCTC	2 cut(s)	793, 2698
Eco47I	GGWCC	7 cut(s)	953, 992, 1307, 1527, 2684, 3052, 3098
Eco53kI	GAGCTC	1 cut(s)	1655
Eco57I	CTGAAG	8 cut(s)	77, 543, 1044, 1860, 1998, 2505, 2574, 3384
Eco72I	CACGTG	1 cut(s)	1718
Eco81I	CCTNAGG	1 cut(s)	1875
Eco88I	CYCGRG	1 cut(s)	972
EcoICRI	GAGCTC	1 cut(s)	1655
EcoO109I	RGGNCCY	1 cut(s)	2665
EcoRI	GAATTC	2 cut(s)	1107, 1685
EcoT14I	CCWWGG	6 cut(s)	199, 949, 998, 1286, 2661, 2709
EcoT22I	ATGCAT	4 cut(s)	6, 268, 1026, 2043
EcoT38I	GRGCYC	2 cut(s)	973, 1657
EgeI	GGCGCC	1 cut(s)	1664
EheI	GGCGCC	1 cut(s)	1664
ErhI	CCWWGG	6 cut(s)	199, 949, 998, 1286, 2661, 2709
Esp3I	CGTCTC	2 cut(s)	2585, 3338
FalI	AAGNNNNNCTT	4 cut(s)	1367, 1399, 1925, 1957
FaqI	GGGAC	6 cut(s)	34, 201, 853, 1330, 2268, 3353
FauI	CCCGC	1 cut(s)	75
FbaI	TGATCA	1 cut(s)	574
FblI	GTMKAC	2 cut(s)	936, 2151
FriOI	GRGCYC	2 cut(s)	973, 1657
FspBI	CTAG	4 cut(s)	465, 944, 1794, 2991
GlaI	GCGC	5 cut(s)	197, 281, 494, 890, 1664
GsaI	CCCAGC	3 cut(s)	1122, 3059, 3164
GsuI	CTGGAG	4 cut(s)	229, 1953, 1984, 3270
HaeII	RGCGCY	3 cut(s)	283, 892, 1666
HapII	CCGG	7 cut(s)	29, 35, 142, 277, 551, 1633, 3341
HgaI	GACGC	2 cut(s)	79, 815
HhaI	GCGC	5 cut(s)	198, 282, 495, 891, 1665
Hin1I	GRCGYC	2 cut(s)	71, 1663
Hin6I	GCGC	5 cut(s)	196, 280, 493, 889, 1663
HinP1I	GCGC	5 cut(s)	196, 280, 493, 889, 1663
HincII	GTYRAC	4 cut(s)	937, 1959, 2106, 2491
HindII	GTYRAC	4 cut(s)	937, 1959, 2106, 2491
HindIII	AAGCTT	1 cut(s)	2111
HpaII	CCGG	7 cut(s)	29, 35, 142, 277, 551, 1633, 3341
Hpy99I	CGWCG	4 cut(s)	73, 483, 504, 941
HpyCH4III	ACNGT	9 cut(s)	341, 647, 719, 1682, 2103, 2579, 3169, 3321, 3457
HpyCH4IV	ACGT	5 cut(s)	1717, 1926, 2417, 2521, 2934
HpySE526I	ACGT	5 cut(s)	1717, 1926, 2417, 2521, 2934
Hsp92I	GRCGYC	2 cut(s)	71, 1663
HspAI	GCGC	5 cut(s)	196, 280, 493, 889, 1663
KasI	GGCGCC	1 cut(s)	1662
Ksp22I	TGATCA	1 cut(s)	574
LguI	GCTCTTC	1 cut(s)	2739
LmnI	GCTCC	9 cut(s)	31, 287, 981, 1522, 1622, 1652, 1771, 2403, 2836
MaeI	CTAG	4 cut(s)	465, 944, 1794, 2991
MaeII	ACGT	5 cut(s)	1717, 1926, 2417, 2521, 2934
MaeIII	GTNAC	5 cut(s)	3042, 3074, 3169, 3475, 3599
MbiI	CCGCTC	1 cut(s)	48
MfeI	CAATTG	2 cut(s)	524, 1644
MflI	RGATCY	5 cut(s)	224, 806, 986, 3220, 3443
MhlI	GDGCHC	4 cut(s)	973, 1657, 1998, 3187
MlsI	TGGCCA	2 cut(s)	1371, 1605
MluNI	TGGCCA	2 cut(s)	1371, 1605
Mly113I	GGCGCC	1 cut(s)	1663
MlyI	GAGTC	9 cut(s)	917, 1765, 2888, 3242, 3277, 3347, 3383, 3400, 3500
MmeI	TCCRAC	4 cut(s)	294, 1783, 2976, 3444
Mox20I	TGGCCA	2 cut(s)	1371, 1605
Mph1103I	ATGCAT	4 cut(s)	6, 268, 1026, 2043
MroXI	GAANNNNTTC	2 cut(s)	2430, 3108
MscI	TGGCCA	2 cut(s)	1371, 1605
MseI	TTAA	7 cut(s)	420, 1097, 1331, 1445, 1823, 2702, 2796
MslI	CAYNNNNRTG	4 cut(s)	1077, 1983, 3458, 3597
Msp20I	TGGCCA	2 cut(s)	1371, 1605
MspA1I	CMGCKG	2 cut(s)	1973, 3160
MspI	CCGG	7 cut(s)	29, 35, 142, 277, 551, 1633, 3341
MunI	CAATTG	2 cut(s)	524, 1644
Mva1269I	GAATGC	1 cut(s)	2921
MvnI	CGCG	1 cut(s)	483
NarI	GGCGCC	1 cut(s)	1663
NciI	CCSGG	2 cut(s)	29, 552
NcoI	CCATGG	1 cut(s)	199
NmeAIII	GCCGAG	1 cut(s)	2140
NmuCI	GTSAC	5 cut(s)	3042, 3074, 3169, 3475, 3599
NruI	TCGCGA	1 cut(s)	483
NsiI	ATGCAT	4 cut(s)	6, 268, 1026, 2043
NspI	RCATGY	6 cut(s)	1024, 1393, 2732, 2916, 3260, 3275
NspV	TTCGAA	1 cut(s)	772
OliI	CACNNNNGTG	2 cut(s)	1983, 3597
PaeI	GCATGC	1 cut(s)	2732
PaqCI	CACCTGC	1 cut(s)	2488
PasI	CCCWGGG	1 cut(s)	966
PceI	AGGCCT	1 cut(s)	2158
PciI	ACATGT	1 cut(s)	2912
PciSI	GCTCTTC	1 cut(s)	2739
PcsI	WCGNNNNNNNCGW	1 cut(s)	1858
PctI	GAATGC	1 cut(s)	2921
PdmI	GAANNNNTTC	2 cut(s)	2430, 3108
PfeI	GAWTC	7 cut(s)	313, 914, 1595, 1916, 2636, 3278, 3311
Pfl23II	CGTACG	1 cut(s)	75
PflFI	GACNNNGTC	1 cut(s)	3455
PfoI	TCCNGGA	1 cut(s)	113
PleI	GAGTC	9 cut(s)	917, 1764, 2888, 3241, 3277, 3347, 3382, 3400, 3499
PluTI	GGCGCC	1 cut(s)	1666
PmaCI	CACGTG	1 cut(s)	1718
PmlI	CACGTG	1 cut(s)	1718
PpsI	GAGTC	9 cut(s)	917, 1764, 2888, 3241, 3277, 3347, 3382, 3400, 3499
Ppu21I	YACGTR	1 cut(s)	1718
PscI	ACATGT	1 cut(s)	2912
PshAI	GACNNNNGTC	1 cut(s)	845
PshBI	ATTAAT	1 cut(s)	1331
PsiI	TTATAA	1 cut(s)	2967
Psp124BI	GAGCTC	1 cut(s)	1657
Psp1406I	AACGTT	2 cut(s)	1926, 2521
PspCI	CACGTG	1 cut(s)	1718
PspFI	CCCAGC	3 cut(s)	1118, 3055, 3160
PspLI	CGTACG	1 cut(s)	75
PspOMI	GGGCCC	1 cut(s)	969
PsrI	GAACNNNNNNTAC	2 cut(s)	3138, 3170
PstNI	CAGNNNCTG	1 cut(s)	2645
PsuI	RGATCY	5 cut(s)	224, 806, 986, 3220, 3443
PsyI	GACNNNGTC	1 cut(s)	3455
PvuII	CAGCTG	2 cut(s)	1973, 3160
RruI	TCGCGA	1 cut(s)	483
RsaI	GTAC	8 cut(s)	77, 556, 2310, 2906, 3030, 3156, 3557, 3635
RsaNI	GTAC	8 cut(s)	76, 555, 2309, 2905, 3029, 3155, 3556, 3634
RseI	CAYNNNNRTG	4 cut(s)	1077, 1983, 3458, 3597
SacI	GAGCTC	1 cut(s)	1657
SalI	GTCGAC	1 cut(s)	935
SapI	GCTCTTC	1 cut(s)	2739
SaqAI	TTAA	7 cut(s)	420, 1097, 1331, 1445, 1823, 2702, 2796
SchI	GAGTC	9 cut(s)	917, 1765, 2888, 3242, 3277, 3347, 3383, 3400, 3500
SduI	GDGCHC	4 cut(s)	973, 1657, 1998, 3187
SfcI	CTRYAG	3 cut(s)	2152, 2496, 3411
SfoI	GGCGCC	1 cut(s)	1664
SfuI	TTCGAA	1 cut(s)	772
SinI	GGWCC	7 cut(s)	953, 992, 1307, 1527, 2684, 3052, 3098
SmiMI	CAYNNNNRTG	4 cut(s)	1077, 1983, 3458, 3597
SmlI	CTYRAG	3 cut(s)	2228, 3090, 3401
SmoI	CTYRAG	3 cut(s)	2228, 3090, 3401
SphI	GCATGC	1 cut(s)	2732
SseBI	AGGCCT	1 cut(s)	2158
SspDI	GGCGCC	1 cut(s)	1662
SspI	AATATT	3 cut(s)	2371, 2451, 2511
SspMI	CTAG	4 cut(s)	465, 944, 1794, 2991
SstI	GAGCTC	1 cut(s)	1657
StuI	AGGCCT	1 cut(s)	2158
StyI	CCWWGG	6 cut(s)	199, 949, 998, 1286, 2661, 2709
TaaI	ACNGT	9 cut(s)	341, 647, 719, 1682, 2103, 2579, 3169, 3321, 3457
TaiI	ACGT	5 cut(s)	1720, 1929, 2420, 2524, 2937
TatI	WGTACW	4 cut(s)	2904, 3028, 3154, 3555
TauI	GCSGC	4 cut(s)	173, 207, 1663, 3505
TfiI	GAWTC	7 cut(s)	313, 914, 1595, 1916, 2636, 3278, 3311
Tru1I	TTAA	7 cut(s)	420, 1097, 1331, 1445, 1823, 2702, 2796
Tru9I	TTAA	7 cut(s)	420, 1097, 1331, 1445, 1823, 2702, 2796
TscAI	CASTG	5 cut(s)	1843, 2287, 2310, 2478, 2607
TseFI	GTSAC	5 cut(s)	3042, 3074, 3169, 3475, 3599
Tsp45I	GTSAC	5 cut(s)	3042, 3074, 3169, 3475, 3599
TspGWI	ACGGA	1 cut(s)	779
TspRI	CASTG	5 cut(s)	1843, 2287, 2310, 2478, 2607
Tth111I	GACNNNGTC	1 cut(s)	3455
VneI	GTGCAC	1 cut(s)	1994
VpaK11BI	GGWCC	7 cut(s)	953, 992, 1307, 1527, 2684, 3052, 3098
VspI	ATTAAT	1 cut(s)	1331
XapI	RAATTY	7 cut(s)	1107, 1545, 1685, 1725, 2234, 2429, 3664
XceI	RCATGY	6 cut(s)	1024, 1393, 2732, 2916, 3260, 3275
XcmI	CCANNNNNNNNNTGG	1 cut(s)	963
XmiI	GTMKAC	2 cut(s)	936, 2151
XmnI	GAANNNNTTC	2 cut(s)	2430, 3108
XspI	CTAG	4 cut(s)	465, 944, 1794, 2991
Zsp2I	ATGCAT	4 cut(s)	6, 268, 1026, 2043

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.