Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0004176

Physical Location & Seq

Genomic Coordinates Forward (+)

26996766 .. 27004350

Gene Structure 7585 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG135400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2607 bp

ATGCCCAGAGAGGATCTATCGGAGATGCATCCGAAGCGATCGGATGGGTCGCCGGCGGCATTACGGAGCAGCGGGCGGATCAGCCGGCGTCCGAAAACTTACTCGCGAAGCATGTTCTATTACAACACGAGTACCAGTAGCATGATTCACAAACGCAGAAGGAAGGCCAAGACCAAGAACCGACCTGCCGCCTCCAGAATTGCTAAGATGATGCGCGATGGCTCCACTCGTTCCCAAAGATCGCCCCACCCCACTTCTCCCACTAAGCTCAATACAAACTTACCCCGCCGCTCCGAAAGAAGGAGAACTCTGTCGGTAAAGAATCCAGATTATGCATCAGACAGTTCCGATGGTGATGAAGACATGATGAAACCCTCTACATGTAAATCTATCAAGAATCGGGGTGCATATCAAGATGAATTATCACCTTCCAAGCACAAAAAGATGGTGGAGAGACCAACACCTCGGCGTGAAGGATTGCGCCCTCGTCGCTCTAAGACTATTTCAAATGAGGATATGCTTTTTGGATATGACGATCTTCCTGTTGATAGTTCGGAAGAGAAGGTTGACCAAGAAGAAACTGAAAATGGGCAGGATATTGAATACAATGATGCAGATGATGGTCAGATTGAGGGCGATGGTGAAGACATGGGTGACGATGATGGAGATGAAGATGGTGATGAAGATGGTGATGATGATGGTGATGATGAAGAGGGCGAAGAGGAACAAGATGGAAGAAGACGTTATGATCTTCGAAATCGTGCAGAAGTCCGCAGGTTCTCTATTGAAGAAGGTAAGCGGAGACCACGATCTCCTCGAAGAGTATTGCACCAGGGAATGGGGCCAAAGGTCAGTAGGGATGTAAGAAAGGGTGGATCACGAGTTCATAAGCGACATCGAATCACAAGGACTGATGATTCTGATGACTCCCTTCTTGTGGATGAGCTCGACCAAGGCCCTGCAATTCCTTGGGGGAAAGGTGGGAGCAGATCTGGACCACCTTGGCTTTTTGGGGGACTAGACATGCATGGAACAACGACATGGGGTTTGAATGTTGCTGCCTCGGGTTGGGGTCACCAGGGTGATGCTTTTGCTACCTTGACTTCTGGAATTCAAACTGCTGGGCCAAGCTCAAAGGGAGGGGCAGACATACAACCTTTGCAGGTTGATGATAGTGTCAGTTTTGAGGATATAGGGGGACTTTCTCAATATATTGATGCTTTGAAGGAAATGGTTTTCTTTCCTTTACTGTATCCAGATTTCTTTGCAAGCTATCATATCACCCCACCAAGGGGAGTTTTGTTATGTGGTCCTCCTGGTACTGGGAAAACATTAATTGCCAGAGCCTTAGCCTCTGCTGCATCCAAGGCTGGCCAGAAAGTGAGTTTTTACATGAGGAAGGGTGCAGATGTCCTAAGCAAGTGGGTTGGCGAGGCTGAAAGACAATTGAAACTTCTTTTTGAGGAAGCACAAAGAAATCAACCTTCAATCATATTCTTTGATGAAATAGATGGACTGGCTCCTGTAAGATCCAGCAAACAAGAGCAAATTCATAATTCTATTGTGTCTACATTGCTTGCTCTGATGGATGGTCTTGATTCCCGTGGACAAGTGGTTTTGATCGGAGCAACCAACCGAATTGATGCAATTGATGGAGCTTTGCGGCGCCCTGGTAGATTTGATCGTGAATTCAACTTTCCTTTGCCTGGCTGTGAGGCCCGTGCTGAAATTTTAGACATTCACAGTCGGAAATGGAAGCATCCTCCGTCAAATGAGTTAAAACTGGAGCTTGCAGCTAGCTGTGTTGGATATTGTGGTGCTGATTTAAAGGCTCTTTGCACTGAAGCTGCTATTCGTGCTTTCCGTGAAAAATATCCTCAGGTCTACACAAGTGATGATAAATTTGTAATTGATGTTGATTCAGTAAGGGTTGAAAAGTATCACTTTGTTGAAGCCATGTCAACAATCACCCCAGCTGCTCACAGAGGTGCTGTTGTGCACTCTAGGCCATTGTCTTTAGTAGTTGCACCATGCCTGCAGAGGCATCTCCAAAGAGCCATGAACTATATATCCGATATTTTCCCTCCGATTGCAGTGTCATCAGAATTGACCAAGCTTTCCATGCTCTCTTGTGGCTCTGCCATTCCTCTTGTTTATAGGCCTCGGCTTCTTCTATGTGGTGGTGAAGGTTCTGGCCTGGATCATCTTGGGCCTGCTATTTTACATGAACTCGAGAAGTTTCCTGTCCATTCTCTAGGACTTCCATCTCTTCTATCTGATCCTAGTGCAAAGACACCAGAGGAAGCACTGGTACATATATTTGGTGAAGCACGGAGGACAACGCCATCAATTCTCTACTTGCCACAGTTCAATCTGTGGTGGGAGACGGCACATGAGCAGCTCCGCGCAGTTTTGCTGACATTGCTAGAAGAATTTCCATCTGATTTACCTATATTACTACTTGCAACATCATCAGTGCCACCTGCTGAAGTTGATGCTATGACCTCTTCAATATTCTCCGAACGTTCTGTGTATGTTGTCCTCATCTTAAAACCTACCAAAATTTTTGAATTCAATTCCTGTGAGACTGATTTTATATGGGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003682 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010639 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031935 GO:0031936 GO:0033043 GO:0033044 GO:0034641 GO:0035510 GO:0040029 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045815 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0080090 GO:0080111 GO:0090304 GO:1901360 GO:1902275 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:2000112 GO:2001141 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Bromodomain

Protein Analysis

868

Amino Acids

96.56

Weight (kDa)

6.0

Isoelectric Point (pI)

62.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IstB_IS21	PF01695	431 - 515	2.4e-07	IstB-like ATP binding protein
AAA	PF00004	433 - 568	3e-39	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	596 - 632	1.2e-10	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05910
fragaria_vesca	FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720
malus_domestica	MD04G1007800.v1.1
prunus_persica	Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1
pyrus_communis	pycom04g03460
rosa_chinensis	RchiOBHm_Chr3g0496841 RchiOBHm_Chr4g0406861
rosa_laevigata	RLG00000008748 RLG00000022493
rosa_multiflora	Rmu_sc0000600.1_g000031 Rmu_sc0001438.1_g000025
rosa_roxburghii	Rroxscaffold_5G00351650 Rroxscaffold_6G00388620
rosa_rugosa	Rorug03G0288300 Rorug04G0075300
rosa_samantha	Rh3AG332000 Rh3BG368600 Rh3CG364900 Rh3DG368000 Rh4AG141500 Rh4BG138300 Rh4CG148200 Rh4DG135400
rosa_wichuraiana	Rw3G029090 Rw4G011520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2491
Acc36I	ACCTGC	4 cut(s)	193, 765, 1153, 2491
AccB1I	GGYRCC	1 cut(s)	1665
AccB7I	CCANNNNNTGG	1 cut(s)	838
AccBSI	CCGCTC	1 cut(s)	291
AccI	GTMKAC	2 cut(s)	1568, 1884
AccII	CGCG	3 cut(s)	106, 216, 2406
AclI	AACGTT	1 cut(s)	2524
AclWI	GGATC	6 cut(s)	21, 86, 883, 1524, 2208, 2273
AcoI	YGGCCR	1 cut(s)	1372
AcsI	RAATTY	8 cut(s)	1110, 1548, 1688, 1728, 1901, 2432, 2562, 2570
AcuI	CTGAAG	2 cut(s)	1863, 2508
AcyI	GRCGYC	2 cut(s)	88, 1666
AfaI	GTAC	3 cut(s)	133, 1321, 2313
AfiI	CCNNNNNNNGG	9 cut(s)	300, 838, 937, 1068, 1290, 1291, 1292, 1322, 1706
AflIII	ACRYGT	1 cut(s)	380
AjnI	CCWGG	6 cut(s)	831, 1077, 1315, 1669, 1705, 2196
AleI	CACNNNNGTG	2 cut(s)	1080, 1986
AloI	GAACNNNNNNTCC	4 cut(s)	867, 899, 2054, 2086
Alw21I	GWGCWC	2 cut(s)	948, 2001
Alw26I	GTCTC	4 cut(s)	448, 796, 2378, 2579
Alw44I	GTGCAC	1 cut(s)	1997
AlwI	GGATC	6 cut(s)	21, 86, 883, 1524, 2208, 2273
Ama87I	CYCGRG	2 cut(s)	1063, 2231
ApaLI	GTGCAC	1 cut(s)	1997
ApeKI	GCWGC	7 cut(s)	69, 1058, 1358, 1793, 1847, 1976, 2398
ApoI	RAATTY	8 cut(s)	1110, 1548, 1688, 1728, 1901, 2432, 2562, 2570
ArsI	GACNNNNNNTTYG	2 cut(s)	1030, 1062
AseI	ATTAAT	1 cut(s)	1334
Asp700I	GAANNNNTTC	1 cut(s)	2433
AspLEI	GCGC	4 cut(s)	216, 483, 1668, 2408
AspS9I	GGNCC	7 cut(s)	842, 956, 995, 1124, 1310, 1717, 2210
AsuII	TTCGAA	1 cut(s)	754
AsuNHI	GCTAGC	1 cut(s)	1796
AvaI	CYCGRG	2 cut(s)	1063, 2231
AvaII	GGWCC	2 cut(s)	995, 1310
AxyI	CCTNAGG	1 cut(s)	1878
BaeGI	GKGCMC	1 cut(s)	2001
BaeI	ACNNNNGTAYC	2 cut(s)	115, 148
BalI	TGGCCA	1 cut(s)	1374
BanI	GGYRCC	1 cut(s)	1665
BanII	GRGCYC	1 cut(s)	948
BarI	GAAGNNNNNNTAC	2 cut(s)	2295, 2327
BauI	CACGAG	2 cut(s)	127, 879
BbsI	GAAGAC	3 cut(s)	366, 651, 745
Bbv12I	GWGCWC	2 cut(s)	948, 2001
BbvI	GCAGC	7 cut(s)	81, 1045, 1345, 1805, 1834, 1963, 2410
BceAI	ACGGC	1 cut(s)	2403
BciT130I	CCWGG	6 cut(s)	833, 1079, 1317, 1671, 1707, 2198
BciVI	GTATCC	1 cut(s)	1263
BcoDI	GTCTC	4 cut(s)	448, 796, 2378, 2579
BfaI	CTAG	7 cut(s)	1019, 1797, 2004, 2255, 2283, 2426, 2605
BfmI	CTRYAG	1 cut(s)	2036
BfoI	RGCGCY	1 cut(s)	1669
BfuAI	ACCTGC	4 cut(s)	193, 765, 1153, 2491
BfuI	GTATCC	1 cut(s)	1263
BglII	AGATCT	1 cut(s)	989
Bme1390I	CCNGG	6 cut(s)	833, 1079, 1317, 1671, 1707, 2198
Bme18I	GGWCC	2 cut(s)	995, 1310
BmeT110I	CYCGRG	2 cut(s)	1063, 2231
BmgT120I	GGNCC	7 cut(s)	842, 956, 995, 1124, 1310, 1717, 2210
BmiI	GGNNCC	4 cut(s)	223, 843, 1521, 1667
BmrFI	CCNGG	6 cut(s)	833, 1079, 1317, 1671, 1707, 2198
BmrI	ACTGGG	1 cut(s)	1332
BmtI	GCTAGC	1 cut(s)	1800
BmuI	ACTGGG	1 cut(s)	1332
BpiI	GAAGAC	3 cut(s)	366, 651, 745
BpmI	CTGGAG	2 cut(s)	178, 1805
Bpu10I	CCTNAGC	2 cut(s)	1348, 1415
Bpu14I	TTCGAA	1 cut(s)	754
BsaHI	GRCGYC	2 cut(s)	88, 1666
BsaI	GGTCTC	2 cut(s)	448, 796
Bsc4I	CCNNNNNNNGG	9 cut(s)	300, 838, 937, 1068, 1290, 1291, 1292, 1322, 1706
Bse118I	RCCGGY	2 cut(s)	52, 84
Bse1I	ACTGG	5 cut(s)	135, 1327, 1521, 1788, 2313
Bse21I	CCTNAGG	1 cut(s)	1878
Bse3DI	GCAATG	2 cut(s)	1571, 2420
BseBI	CCWGG	6 cut(s)	833, 1079, 1317, 1671, 1707, 2198
BseGI	GGATG	7 cut(s)	28, 49, 865, 946, 1361, 1594, 1759
BseLI	CCNNNNNNNGG	9 cut(s)	300, 838, 937, 1068, 1290, 1291, 1292, 1322, 1706
BseMI	GCAATG	2 cut(s)	1571, 2420
BseMII	CTCAG	1 cut(s)	1892
BseNI	ACTGG	5 cut(s)	135, 1327, 1521, 1788, 2313
BseRI	GAGGAG	1 cut(s)	804
BseSI	GKGCMC	1 cut(s)	2001
BseXI	GCAGC	7 cut(s)	81, 1045, 1345, 1805, 1834, 1963, 2410
BseYI	CCCAGC	2 cut(s)	1121, 1972
BsgI	GTGCAG	2 cut(s)	783, 1425
Bsh1236I	CGCG	3 cut(s)	106, 216, 2406
Bsh1285I	CGRYCG	1 cut(s)	41
BshNI	GGYRCC	1 cut(s)	1665
BsiEI	CGRYCG	1 cut(s)	41
BsiHKAI	GWGCWC	2 cut(s)	948, 2001
BsiHKCI	CYCGRG	2 cut(s)	1063, 2231
BsiSI	CCGG	2 cut(s)	53, 85
BslFI	GGGAC	2 cut(s)	1029, 1212
BslI	CCNNNNNNNGG	9 cut(s)	300, 838, 937, 1068, 1290, 1291, 1292, 1322, 1706
BsmAI	GTCTC	4 cut(s)	448, 796, 2378, 2579
BsmBI	CGTCTC	1 cut(s)	2378
BsmFI	GGGAC	2 cut(s)	1029, 1212
Bso31I	GGTCTC	2 cut(s)	448, 796
BsoBI	CYCGRG	2 cut(s)	1063, 2231
Bsp119I	TTCGAA	1 cut(s)	754
Bsp1286I	GDGCHC	2 cut(s)	948, 2001
Bsp68I	TCGCGA	1 cut(s)	106
BspCNI	CTCAG	1 cut(s)	1891
BspFNI	CGCG	3 cut(s)	106, 216, 2406
BspLI	GGNNCC	4 cut(s)	223, 843, 1521, 1667
BspMAI	CTGCAG	1 cut(s)	2040
BspMI	ACCTGC	4 cut(s)	193, 765, 1153, 2491
BspOI	GCTAGC	1 cut(s)	1800
BspPI	GGATC	6 cut(s)	21, 86, 883, 1524, 2208, 2273
BspT104I	TTCGAA	1 cut(s)	754
BspT107I	GGYRCC	1 cut(s)	1665
BspTNI	GGTCTC	2 cut(s)	448, 796
BsrBI	CCGCTC	1 cut(s)	291
BsrDI	GCAATG	2 cut(s)	1571, 2420
BsrFI	RCCGGY	2 cut(s)	52, 84
BsrI	ACTGG	5 cut(s)	135, 1327, 1521, 1788, 2313
BssAI	RCCGGY	2 cut(s)	52, 84
BssNI	GRCGYC	2 cut(s)	88, 1666
BssSI	CACGAG	2 cut(s)	127, 879
BssT1I	CCWWGG	5 cut(s)	952, 968, 1001, 1289, 1365
Bst2BI	CACGAG	2 cut(s)	127, 879
Bst2UI	CCWGG	6 cut(s)	833, 1079, 1317, 1671, 1707, 2198
Bst4CI	ACNGT	4 cut(s)	344, 1251, 1745, 2367
Bst6I	CTCTTC	6 cut(s)	552, 705, 714, 814, 2274, 2512
BstACI	GRCGYC	2 cut(s)	88, 1666
BstBI	TTCGAA	1 cut(s)	754
BstDEI	CTNAG	6 cut(s)	204, 264, 495, 1348, 1415, 1878
BstDSI	CCRYGG	1 cut(s)	1603
BstEII	GGTNACC	1 cut(s)	1073
BstF5I	GGATG	7 cut(s)	28, 49, 865, 946, 1361, 1594, 1759
BstFNI	CGCG	3 cut(s)	106, 216, 2406
BstH2I	RGCGCY	1 cut(s)	1669
BstHHI	GCGC	4 cut(s)	216, 483, 1668, 2408
BstMAI	GTCTC	4 cut(s)	448, 796, 2378, 2579
BstMCI	CGRYCG	1 cut(s)	41
BstMWI	GCNNNNNNNGC	8 cut(s)	34, 489, 1358, 1367, 1856, 2005, 2122, 2422
BstNI	CCWGG	6 cut(s)	833, 1079, 1317, 1671, 1707, 2198
BstNSI	RCATGY	3 cut(s)	115, 384, 1027
BstPI	GGTNACC	1 cut(s)	1073
BstSCI	CCNGG	6 cut(s)	831, 1077, 1315, 1669, 1705, 2196
BstSFI	CTRYAG	1 cut(s)	2036
BstSLI	GKGCMC	1 cut(s)	2001
BstUI	CGCG	3 cut(s)	106, 216, 2406
BstV1I	GCAGC	7 cut(s)	81, 1045, 1345, 1805, 1834, 1963, 2410
BstV2I	GAAGAC	3 cut(s)	366, 651, 745
BstX2I	RGATCY	3 cut(s)	13, 989, 1529
BstYI	RGATCY	3 cut(s)	13, 989, 1529
Bsu36I	CCTNAGG	1 cut(s)	1878
BsuI	GTATCC	1 cut(s)	1263
BtgI	CCRYGG	1 cut(s)	1603
BtgZI	GCGATG	2 cut(s)	231, 651
BtsCI	GGATG	7 cut(s)	28, 49, 865, 946, 1361, 1594, 1759
BtsI	GCAGTG	1 cut(s)	2100
BtsIMutI	CAGTG	4 cut(s)	1839, 2100, 2306, 2481
BtuMI	TCGCGA	1 cut(s)	106
BveI	ACCTGC	4 cut(s)	193, 765, 1153, 2491
CfoI	GCGC	4 cut(s)	216, 483, 1668, 2408
Cfr10I	RCCGGY	2 cut(s)	52, 84
Cfr13I	GGNCC	7 cut(s)	842, 956, 995, 1124, 1310, 1717, 2210
CseI	GACGC	1 cut(s)	77
Csp6I	GTAC	3 cut(s)	132, 1320, 2312
CviQI	GTAC	3 cut(s)	132, 1320, 2312
DdeI	CTNAG	6 cut(s)	204, 264, 495, 1348, 1415, 1878
DinI	GGCGCC	1 cut(s)	1667
DraI	TTTAAA	1 cut(s)	1827
EaeI	YGGCCR	1 cut(s)	1372
Eam1104I	CTCTTC	6 cut(s)	552, 705, 714, 814, 2274, 2512
EarI	CTCTTC	6 cut(s)	552, 705, 714, 814, 2274, 2512
EciI	GGCGGA	1 cut(s)	91
Ecl136II	GAGCTC	1 cut(s)	946
Eco130I	CCWWGG	5 cut(s)	952, 968, 1001, 1289, 1365
Eco147I	AGGCCT	1 cut(s)	2161
Eco24I	GRGCYC	1 cut(s)	948
Eco31I	GGTCTC	2 cut(s)	448, 796
Eco47I	GGWCC	2 cut(s)	995, 1310
Eco53kI	GAGCTC	1 cut(s)	946
Eco57I	CTGAAG	2 cut(s)	1863, 2508
Eco81I	CCTNAGG	1 cut(s)	1878
Eco88I	CYCGRG	2 cut(s)	1063, 2231
Eco91I	GGTNACC	1 cut(s)	1073
EcoICRI	GAGCTC	1 cut(s)	946
EcoO109I	RGGNCCY	1 cut(s)	956
EcoO65I	GGTNACC	1 cut(s)	1073
EcoRI	GAATTC	3 cut(s)	1110, 1688, 2570
EcoRII	CCWGG	6 cut(s)	831, 1077, 1315, 1669, 1705, 2196
EcoT14I	CCWWGG	5 cut(s)	952, 968, 1001, 1289, 1365
EcoT22I	ATGCAT	3 cut(s)	30, 337, 1029
EcoT38I	GRGCYC	1 cut(s)	948
EgeI	GGCGCC	1 cut(s)	1667
EheI	GGCGCC	1 cut(s)	1667
ErhI	CCWWGG	5 cut(s)	952, 968, 1001, 1289, 1365
Esp3I	CGTCTC	1 cut(s)	2378
FalI	AAGNNNNNCTT	2 cut(s)	1928, 1960
FaqI	GGGAC	2 cut(s)	1029, 1212
FauI	CCCGC	2 cut(s)	65, 293
FblI	GTMKAC	2 cut(s)	1568, 1884
FokI	GGATG	7 cut(s)	15, 56, 872, 953, 1348, 1601, 1746
FriOI	GRGCYC	1 cut(s)	948
FspBI	CTAG	7 cut(s)	1019, 1797, 2004, 2255, 2283, 2426, 2605
GlaI	GCGC	4 cut(s)	215, 482, 1667, 2407
GsaI	CCCAGC	2 cut(s)	1125, 1976
GsuI	CTGGAG	2 cut(s)	178, 1805
HaeII	RGCGCY	1 cut(s)	1669
HapII	CCGG	2 cut(s)	53, 85
HgaI	GACGC	1 cut(s)	77
HhaI	GCGC	4 cut(s)	216, 483, 1668, 2408
Hin1I	GRCGYC	2 cut(s)	88, 1666
Hin6I	GCGC	4 cut(s)	214, 481, 1666, 2406
HinP1I	GCGC	4 cut(s)	214, 481, 1666, 2406
HincII	GTYRAC	2 cut(s)	568, 1962
HindII	GTYRAC	2 cut(s)	568, 1962
HindIII	AAGCTT	1 cut(s)	2114
HinfI	GANTC	8 cut(s)	145, 322, 397, 900, 917, 926, 1598, 1919
HpaII	CCGG	2 cut(s)	53, 85
Hpy166II	GTNNAC	6 cut(s)	568, 1569, 1607, 1885, 1962, 1999
Hpy8I	GTNNAC	6 cut(s)	568, 1569, 1607, 1885, 1962, 1999
Hpy99I	CGWCG	1 cut(s)	492
HpyCH4III	ACNGT	4 cut(s)	344, 1251, 1745, 2367
HpyCH4IV	ACGT	2 cut(s)	742, 2524
HpyF10VI	GCNNNNNNNGC	8 cut(s)	34, 489, 1358, 1367, 1856, 2005, 2122, 2422
HpyF3I	CTNAG	6 cut(s)	204, 264, 495, 1348, 1415, 1878
HpySE526I	ACGT	2 cut(s)	742, 2524
Hsp92I	GRCGYC	2 cut(s)	88, 1666
HspAI	GCGC	4 cut(s)	214, 481, 1666, 2406
KasI	GGCGCC	1 cut(s)	1665
KroI	GCCGGC	2 cut(s)	52, 84
KroNI	GCCGGC	2 cut(s)	54, 86
LmnI	GCTCC	9 cut(s)	66, 227, 296, 984, 1525, 1625, 1655, 1786, 2406
Lsp1109I	GCAGC	7 cut(s)	81, 1045, 1345, 1805, 1834, 1963, 2410
MaeI	CTAG	7 cut(s)	1019, 1797, 2004, 2255, 2283, 2426, 2605
MaeII	ACGT	2 cut(s)	742, 2524
MaeIII	GTNAC	2 cut(s)	653, 1073
MbiI	CCGCTC	1 cut(s)	291
MfeI	CAATTG	2 cut(s)	1445, 1647
MflI	RGATCY	3 cut(s)	13, 989, 1529
MhlI	GDGCHC	2 cut(s)	948, 2001
MlsI	TGGCCA	1 cut(s)	1374
MluNI	TGGCCA	1 cut(s)	1374
Mly113I	GGCGCC	1 cut(s)	1666
MlyI	GAGTC	1 cut(s)	920
MmeI	TCCRAC	2 cut(s)	1727, 1786
Mox20I	TGGCCA	1 cut(s)	1374
Mph1103I	ATGCAT	3 cut(s)	30, 337, 1029
MreI	CGCCGGCG	1 cut(s)	52
MroNI	GCCGGC	2 cut(s)	52, 84
MroXI	GAANNNNTTC	1 cut(s)	2433
MscI	TGGCCA	1 cut(s)	1374
MseI	TTAA	4 cut(s)	1334, 1778, 1826, 2549
MslI	CAYNNNNRTG	2 cut(s)	1080, 1986
Msp20I	TGGCCA	1 cut(s)	1374
MspA1I	CMGCKG	2 cut(s)	72, 1976
MspI	CCGG	2 cut(s)	53, 85
MspR9I	CCNGG	6 cut(s)	833, 1079, 1317, 1671, 1707, 2198
MunI	CAATTG	2 cut(s)	1445, 1647
MvaI	CCWGG	6 cut(s)	833, 1079, 1317, 1671, 1707, 2198
MvnI	CGCG	3 cut(s)	106, 216, 2406
MwoI	GCNNNNNNNGC	8 cut(s)	34, 489, 1358, 1367, 1856, 2005, 2122, 2422
NaeI	GCCGGC	2 cut(s)	54, 86
NarI	GGCGCC	1 cut(s)	1666
NgoMIV	GCCGGC	2 cut(s)	52, 84
NheI	GCTAGC	1 cut(s)	1796
NlaIV	GGNNCC	4 cut(s)	223, 843, 1521, 1667
NmeAIII	GCCGAG	2 cut(s)	445, 2143
NmuCI	GTSAC	2 cut(s)	653, 1073
NruI	TCGCGA	1 cut(s)	106
NsiI	ATGCAT	3 cut(s)	30, 337, 1029
NspI	RCATGY	3 cut(s)	115, 384, 1027
NspV	TTCGAA	1 cut(s)	754
OliI	CACNNNNGTG	2 cut(s)	1080, 1986
PaeR7I	CTCGAG	1 cut(s)	2231
PaqCI	CACCTGC	1 cut(s)	2491
PceI	AGGCCT	1 cut(s)	2161
PciI	ACATGT	1 cut(s)	380
PcsI	WCGNNNNNNNCGW	2 cut(s)	814, 1861
PdiI	GCCGGC	2 cut(s)	54, 86
PdmI	GAANNNNTTC	1 cut(s)	2433
PfeI	GAWTC	7 cut(s)	145, 322, 397, 900, 917, 1598, 1919
PflMI	CCANNNNNTGG	1 cut(s)	838
Ple19I	CGATCG	1 cut(s)	41
PleI	GAGTC	1 cut(s)	920
PluTI	GGCGCC	1 cut(s)	1669
PpsI	GAGTC	1 cut(s)	920
PscI	ACATGT	1 cut(s)	380
PshBI	ATTAAT	1 cut(s)	1334
Psp124BI	GAGCTC	1 cut(s)	948
Psp1406I	AACGTT	1 cut(s)	2524
Psp6I	CCWGG	6 cut(s)	831, 1077, 1315, 1669, 1705, 2196
PspEI	GGTNACC	1 cut(s)	1073
PspFI	CCCAGC	2 cut(s)	1121, 1972
PspGI	CCWGG	6 cut(s)	831, 1077, 1315, 1669, 1705, 2196
PspN4I	GGNNCC	4 cut(s)	223, 843, 1521, 1667
PspPI	GGNCC	7 cut(s)	842, 956, 995, 1124, 1310, 1717, 2210
PstI	CTGCAG	1 cut(s)	2040
PsuI	RGATCY	3 cut(s)	13, 989, 1529
PvuI	CGATCG	1 cut(s)	41
PvuII	CAGCTG	1 cut(s)	1976
RruI	TCGCGA	1 cut(s)	106
RsaI	GTAC	3 cut(s)	133, 1321, 2313
RsaNI	GTAC	3 cut(s)	132, 1320, 2312
RseI	CAYNNNNRTG	2 cut(s)	1080, 1986
SacI	GAGCTC	1 cut(s)	948
SaqAI	TTAA	4 cut(s)	1334, 1778, 1826, 2549
Sau96I	GGNCC	7 cut(s)	842, 956, 995, 1124, 1310, 1717, 2210
SchI	GAGTC	1 cut(s)	920
ScrFI	CCNGG	6 cut(s)	833, 1079, 1317, 1671, 1707, 2198
SduI	GDGCHC	2 cut(s)	948, 2001
SfcI	CTRYAG	1 cut(s)	2036
SfoI	GGCGCC	1 cut(s)	1667
Sfr274I	CTCGAG	1 cut(s)	2231
SfuI	TTCGAA	1 cut(s)	754
SgrAI	CRCCGGYG	1 cut(s)	52
SinI	GGWCC	2 cut(s)	995, 1310
SlaI	CTCGAG	1 cut(s)	2231
SmiMI	CAYNNNNRTG	2 cut(s)	1080, 1986
SmlI	CTYRAG	1 cut(s)	2231
SmoI	CTYRAG	1 cut(s)	2231
SseBI	AGGCCT	1 cut(s)	2161
SspDI	GGCGCC	1 cut(s)	1665
SspI	AATATT	1 cut(s)	2514
SspMI	CTAG	7 cut(s)	1019, 1797, 2004, 2255, 2283, 2426, 2605
SstI	GAGCTC	1 cut(s)	948
StuI	AGGCCT	1 cut(s)	2161
StyD4I	CCNGG	6 cut(s)	831, 1077, 1315, 1669, 1705, 2196
StyI	CCWWGG	5 cut(s)	952, 968, 1001, 1289, 1365
TaaI	ACNGT	4 cut(s)	344, 1251, 1745, 2367
TaiI	ACGT	2 cut(s)	745, 2527
TaqI	TCGA	5 cut(s)	754, 817, 898, 948, 2232
TauI	GCSGC	4 cut(s)	59, 191, 291, 1666
TfiI	GAWTC	7 cut(s)	145, 322, 397, 900, 917, 1598, 1919
Tru1I	TTAA	4 cut(s)	1334, 1778, 1826, 2549
Tru9I	TTAA	4 cut(s)	1334, 1778, 1826, 2549
TscAI	CASTG	4 cut(s)	1846, 2100, 2313, 2481
TseFI	GTSAC	2 cut(s)	653, 1073
TseI	GCWGC	7 cut(s)	69, 1058, 1358, 1793, 1847, 1976, 2398
Tsp45I	GTSAC	2 cut(s)	653, 1073
TspGWI	ACGGA	4 cut(s)	79, 1755, 1853, 2347
TspRI	CASTG	4 cut(s)	1846, 2100, 2313, 2481
Van91I	CCANNNNNTGG	1 cut(s)	838
VneI	GTGCAC	1 cut(s)	1997
VpaK11BI	GGWCC	2 cut(s)	995, 1310
VspI	ATTAAT	1 cut(s)	1334
XapI	RAATTY	8 cut(s)	1110, 1548, 1688, 1728, 1901, 2432, 2562, 2570
XceI	RCATGY	3 cut(s)	115, 384, 1027
XhoI	CTCGAG	1 cut(s)	2231
XmiI	GTMKAC	2 cut(s)	1568, 1884
XmnI	GAANNNNTTC	1 cut(s)	2433
XspI	CTAG	7 cut(s)	1019, 1797, 2004, 2255, 2283, 2426, 2605
Zsp2I	ATGCAT	3 cut(s)	30, 337, 1029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.