Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0004176

Physical Location & Seq

Genomic Coordinates Forward (+)

28135682 .. 28142648

Gene Structure 6967 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00351650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3492 bp

ATGCCCAGAGAGGATCTATCGGAGATGCATCCGAAACGATCGGATGGGTCGCCGGCGGCATTACGGAGCAGCGGGCGGATCAGCCGGCGTCCGAAAACTTACTCGCGAAGCATGTTCTATTACAACACGAGTACCAGTAGCATGATTCACAAACGCAGAAGGAAGGCCAAGACCAAGAACCGACCTGCCGCCTCCAGAATTGCTAAGATGATGCGCGATGGCTCCACTCGTTCCCAAAGATCGCCCCACCCCACTTCTCCCACTAAGCTCAATACAAACTTACCCCGCCGCTCCGAAAGAAGGAGAACTCTGTCTGTAAAGAATCCAGATTATGCATCAGACAGTTCCGATGGTGATGAAGACATGATGAAACCCTCTACATGTAAATCTATCAAGAATCGGGGTGCATATCAAGATGAATTATCACCTTCCAAGCACAAAAAGATGGTGGAGAGACCAACACCTCGGCGTGAAGGATTGCGCCCTCGTCGCTCTAAGACTATTTCAAATGAGGATATACTTTTTGGATATGACGATCTTCCTGTTGATAGTTCGGAAGAGAAGGTTGACCAAGAAGAAACCGAAAATGGGCAGGATATTGAATACAATGATGCAGATGATGGTCAGAATGAGGGCGATGGTGAAGACATGGGTGATGATGATGGCGATGAAGATGGTGATGAAGATGGTGATGATGATGGTGATGACGAAGAGGGCGAAGAGGAACAAGATGGAAGAAGACGTTATGATCTTCGAAATCGTGCAGAAGTCCGCAGGTTCTCTATTGAAGAAGGTAAGCGGAGACCACGATCTCCTCGAAGAGTATTGCACCAAGGAATGGGGCCAAAGGTCGGTAGGGATGTAAGAAAGGGTGGATCACGAGTTCATAAGCGTCATCGAATCACAAGGACTGATGATTCTGATGACTCCCTTCTTGTGGATGAGCTCGACCAAGGCCCTGCAATTCCCTGGGGGAAAGGTGGGAGCAGATCTGGACCCCCTTGGCTTTTTGGGGGACTAGACATGCATGGAACAACGACATGGGGTTTGAATGTTGCTGCCTCGGGTTGGGGTCACCAGGGTGATGCTTTTGCTACCTTGACTTCTGGAATTCAAACTGCTGGGCCAAGCTCAAAGGGAGGGGCAGACATACAACCTTTGCAGGTTGATGATAGTGTCAGTTTTGAGGATATAGGGGGACTTTCTCAATATATTGATGCTTTGAAGGAAATGGTTTTCTTTCCTTTACTGTATCCAGATTTCTTTGCAAGCTATCATATCACCCCACCAAGGGGAGTTTTGTTGTGTGGTCCTCCTGGTACTGGGAAAACGTTAATTGCCAGAGCCTTAGCCTCTGCTGCATCCAAGGCTGGCCAGAAAGTGAGTTTTTACATGAGGAAGGGTGCAGATGTCCTAAGCAAGTGGGTTGGCGAGGCTGAAAGACAATTGAAACTTCTTTTTGAGGAAGCACAAAGAAATCAACCTTCAATCATATTCTTTGATGAAATAGATGGACTGGCTCCTGTAAGATCCAGCAAACAAGAGCAAATTCATAATTCTATTGTGTCTACATTGCTTGCTCTGATGGATGGTCTTGATTCCCGTGGACAAGTGGTTTTGATCGGAGCAACCAACCGAATTGATGCAATTGATGGAGCTTTGCGGCGCCCTGGTAGATTTGATCGTGAATTCAACTTTCCTTTGCCTGGCTGTGAGGCCCGTGCTGAAATTTTAGACATTCACAGTCGGAAATGGAAGCATCCTCCTTCAAATGAGTTAAAACTGGAGCTTGCAGCTAGCTGTGTTGGATATTGTGGTGCTGATTTAAAGGCTCTTTGCACTGAAGCTGCTATTCGTGCTTTCCGTGAAAAATATCCTCAGGTCTACACAAGTGATGATAAATTTGTAATTGATGTTGATTCAGTAAGGGTTGAAAAGTATCACTTTGTTGAAGCCATGTCAACAATCACCCCAGCTGCTCACAGAGGTGCTGTTGTGCACTCTAGGCCATTGTCTTTAGTAGTCGCACCATGCCTGCAGAGGCATCTCCAAAGAGCCATGAACTATATATCCGATATTTTCCCTCCGATTGCAGTGTCATCAGAATTGACCAAGCTTTCCATGCTCTCTTGTGGTTCTGCCATTCCTCTTGTTTATAGGCCTCGGCTTCTTCTATGTGGTGGTGAAGGTTCTGGCCTGGCACATGAGCAGCTCCGCGCAGTTTTGCTGACATTGCTAGAAGAATTTCCATCTGATTTACCTATATTACTACTTGCAACATCATCAGTGCCACCTGCTGAAGTTGATGCTATGACCTCTTCAATATTCTCCGAACGTTCTGTCTATCAGGTGGATACGCCTTCTACTGAAGACAGATCTTTGTTTTTTGACCGTCTGATTGAAGCTGCCTTGTCAGTCTTGTTGGAGGGCACAACTAAAAGGTCTCAGGAATCAGTATCTGTTCCTGAACTTCCAAAGGCTCCAAAAGTGGCAAGCGGTCCAAAGGTGTCAGAGTTAAAAGCCAAGGTAGAAGCTGAGCAGCATGCTCTTCGCCGATTGCGGATGTGCCTCAGAGATGTTTGCAATAGAATTCTATACGATAAGAGGTTTAGTGCTTTTCACTATCCTGTCTTGGATGAGGATGCTCCAAACTATCGCTCAATTATCCAAAATCCTATGGATGTTGCAACTCTGCTGCAGCGTGTTGACTCTGGGCTGTATATTACATGTTCAGCATTCCTGCAAGATGTTGATCTTATAGTTTCTAATGCAAAGGCTTATAATGGAGATGATTACAATGGTGCTAGGATTGTTAGTAGAGGTTATGAGCTTCGAGATGCAGTACATGGAATGCTGTCACAGATGGACCCTGCACTGATTGCATATTGTGACAAGATTGCTGCTCAAGGTGGTCCAGAGCAAATACCTGAAGATTTAGGGGTAGCTACTTTCCCATCAATACCTGTCGTACAGCTGGGAACTGTCACTAGGGCGAGTGCTCGACTTCGTAATGTGCAGCTGGAGGTAAATCTGGATCATAGCTACGAGGCACACAAACGGCCGAAGAAGAATATTGATGCCGCACCTGCAGCTTCAACAGCAGAAGACAAGTCACAGCATCAAGGTTCTGTACCGTCCACGTCATCTCAGGAACCCGAGATGAATAACACAGATCTTGGAGTGCCAGAAACTTCCTCTGTTGTTCTCAATCAGCTTGAAACTTCTGAAGTAGTAGAAGTTTCTGGCCACGCTGATGCGAGTGGATCTGAAGACATCAAGATGTTAGATGGTGAGATTACAAACCAAATGGAGTCTGTCAAGCGGCTTTTTGTGGAGCGGACAAAAACTTATGACATTCCACAGCTTGAGAGGCTTTATGCAAGAATTATGAAGGGCATTTTCGACATCAAGGACAAAAGCGATATAGATGGCACCAAGCATTTAATTTTGAAGTATTTGTTGAAATTTGCAGAGGATGAAGCAAATTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003682 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010639 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031935 GO:0031936 GO:0033043 GO:0033044 GO:0034641 GO:0035510 GO:0040029 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045815 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0080090 GO:0080111 GO:0090304 GO:1901360 GO:1902275 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:2000112 GO:2001141 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Bromodomain

Protein Analysis

1163

Amino Acids

128.9

Weight (kDa)

5.79

Isoelectric Point (pI)

56.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IstB_IS21	PF01695	431 - 515	3.4e-07	IstB-like ATP binding protein
AAA	PF00004	433 - 568	4.6e-39	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	596 - 632	1.7e-10	AAA+ lid domain
Bromodomain	PF00439	861 - 931	1.9e-13	Bromodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05910
fragaria_vesca	FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720
malus_domestica	MD04G1007800.v1.1
prunus_persica	Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1
pyrus_communis	pycom04g03460
rosa_chinensis	RchiOBHm_Chr3g0496841 RchiOBHm_Chr4g0406861
rosa_laevigata	RLG00000008748 RLG00000022493
rosa_multiflora	Rmu_sc0000600.1_g000031 Rmu_sc0001438.1_g000025
rosa_roxburghii	Rroxscaffold_5G00351650 Rroxscaffold_6G00388620
rosa_rugosa	Rorug03G0288300 Rorug04G0075300
rosa_samantha	Rh3AG332000 Rh3BG368600 Rh3CG364900 Rh3DG368000 Rh4AG141500 Rh4BG138300 Rh4CG148200 Rh4DG135400
rosa_wichuraiana	Rw3G029090 Rw4G011520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2781
AarI	CACCTGC	2 cut(s)	2302, 3094
Acc36I	ACCTGC	5 cut(s)	193, 765, 1153, 2302, 3094
AccB1I	GGYRCC	2 cut(s)	1665, 3431
AccB7I	CCANNNNNTGG	1 cut(s)	838
AccBSI	CCGCTC	2 cut(s)	291, 3337
AccI	GTMKAC	2 cut(s)	1568, 1884
AccII	CGCG	3 cut(s)	106, 216, 2217
AclI	AACGTT	2 cut(s)	1331, 2335
AclWI	GGATC	6 cut(s)	21, 86, 883, 1524, 3042, 3271
AcoI	YGGCCR	3 cut(s)	1372, 3059, 3244
AcsI	RAATTY	9 cut(s)	1110, 1548, 1688, 1728, 1901, 2243, 2589, 3464, 3484
AcuI	CTGAAG	6 cut(s)	1863, 2319, 2388, 2949, 3246, 3288
AcyI	GRCGYC	2 cut(s)	88, 1666
AfaI	GTAC	5 cut(s)	133, 1321, 2844, 2970, 3132
AflIII	ACRYGT	2 cut(s)	380, 2726
AjiI	CACGTC	1 cut(s)	3141
AjnI	CCWGG	6 cut(s)	968, 1077, 1315, 1669, 1705, 2196
AleI	CACNNNNGTG	2 cut(s)	1080, 1986
AloI	GAACNNNNNNTCC	4 cut(s)	867, 899, 2054, 2086
Alw21I	GWGCWC	3 cut(s)	948, 2001, 3001
Alw26I	GTCTC	3 cut(s)	448, 796, 2448
Alw44I	GTGCAC	1 cut(s)	1997
AlwI	GGATC	6 cut(s)	21, 86, 883, 1524, 3042, 3271
AlwNI	CAGNNNCTG	1 cut(s)	2459
Ama87I	CYCGRG	2 cut(s)	1063, 3155
ApaLI	GTGCAC	1 cut(s)	1997
ApoI	RAATTY	9 cut(s)	1110, 1548, 1688, 1728, 1901, 2243, 2589, 3464, 3484
ArsI	GACNNNNNNTTYG	4 cut(s)	1030, 1062, 2325, 2357
Asp700I	GAANNNNTTC	1 cut(s)	2244
AspLEI	GCGC	4 cut(s)	216, 483, 1668, 2219
AspS9I	GGNCC	9 cut(s)	842, 956, 995, 1124, 1310, 1717, 2498, 2866, 2912
AsuII	TTCGAA	1 cut(s)	754
AsuNHI	GCTAGC	1 cut(s)	1796
AvaI	CYCGRG	2 cut(s)	1063, 3155
AvaII	GGWCC	5 cut(s)	995, 1310, 2498, 2866, 2912
AxyI	CCTNAGG	1 cut(s)	1878
BaeGI	GKGCMC	2 cut(s)	2001, 2432
BaeI	ACNNNNGTAYC	2 cut(s)	115, 148
BalI	TGGCCA	2 cut(s)	1374, 3246
BanI	GGYRCC	2 cut(s)	1665, 3431
BanII	GRGCYC	1 cut(s)	948
BauI	CACGAG	2 cut(s)	127, 879
BbsI	GAAGAC	6 cut(s)	366, 651, 745, 2376, 3111, 3276
Bbv12I	GWGCWC	3 cut(s)	948, 2001, 3001
BceAI	ACGGC	1 cut(s)	3074
BcgI	CGANNNNNNTGC	2 cut(s)	2531, 2565
BciT130I	CCWGG	6 cut(s)	970, 1079, 1317, 1671, 1707, 2198
BciVI	GTATCC	2 cut(s)	1263, 2347
BcoDI	GTCTC	3 cut(s)	448, 796, 2448
BfaI	CTAG	6 cut(s)	1019, 1797, 2004, 2237, 2805, 2988
BfmI	CTRYAG	3 cut(s)	2036, 2696, 3087
BfoI	RGCGCY	1 cut(s)	1669
BfuAI	ACCTGC	5 cut(s)	193, 765, 1153, 2302, 3094
BfuI	GTATCC	2 cut(s)	1263, 2347
BglII	AGATCT	3 cut(s)	989, 2375, 3172
BlpI	GCTNAGC	1 cut(s)	2535
Bme1390I	CCNGG	6 cut(s)	970, 1079, 1317, 1671, 1707, 2198
Bme18I	GGWCC	5 cut(s)	995, 1310, 2498, 2866, 2912
BmeT110I	CYCGRG	2 cut(s)	1063, 3155
BmgBI	CACGTC	1 cut(s)	3141
BmgT120I	GGNCC	9 cut(s)	842, 956, 995, 1124, 1310, 1717, 2498, 2866, 2912
BmiI	GGNNCC	9 cut(s)	223, 843, 997, 1521, 1667, 2481, 2868, 3153, 3433
BmrFI	CCNGG	6 cut(s)	970, 1079, 1317, 1671, 1707, 2198
BmrI	ACTGGG	1 cut(s)	1332
BmtI	GCTAGC	1 cut(s)	1800
BmuI	ACTGGG	1 cut(s)	1332
BpiI	GAAGAC	6 cut(s)	366, 651, 745, 2376, 3111, 3276
BpmI	CTGGAG	3 cut(s)	178, 1805, 3041
Bpu10I	CCTNAGC	2 cut(s)	1348, 1415
Bpu1102I	GCTNAGC	1 cut(s)	2535
Bpu14I	TTCGAA	1 cut(s)	754
BpuEI	CTTGAG	2 cut(s)	2889, 3386
BsaBI	GATNNNNATC	1 cut(s)	2751
BsaHI	GRCGYC	2 cut(s)	88, 1666
BsaI	GGTCTC	3 cut(s)	448, 796, 2448
Bse118I	RCCGGY	2 cut(s)	52, 84
Bse1I	ACTGG	4 cut(s)	135, 1327, 1521, 1788
Bse21I	CCTNAGG	1 cut(s)	1878
Bse3DI	GCAATG	2 cut(s)	1571, 2231
Bse8I	GATNNNNATC	1 cut(s)	2751
BseBI	CCWGG	6 cut(s)	970, 1079, 1317, 1671, 1707, 2198
BseJI	GATNNNNATC	1 cut(s)	2751
BseMI	GCAATG	2 cut(s)	1571, 2231
BseMII	CTCAG	5 cut(s)	1892, 2459, 2526, 2584, 3161
BseNI	ACTGG	4 cut(s)	135, 1327, 1521, 1788
BseRI	GAGGAG	1 cut(s)	804
BseSI	GKGCMC	2 cut(s)	2001, 2432
BseX3I	CGGCCG	1 cut(s)	3059
BseYI	CCCAGC	3 cut(s)	1121, 1972, 2974
BsgI	GTGCAG	4 cut(s)	783, 1425, 2856, 3035
Bsh1236I	CGCG	3 cut(s)	106, 216, 2217
Bsh1285I	CGRYCG	2 cut(s)	41, 3062
BshNI	GGYRCC	2 cut(s)	1665, 3431
BsiEI	CGRYCG	2 cut(s)	41, 3062
BsiHKAI	GWGCWC	3 cut(s)	948, 2001, 3001
BsiHKCI	CYCGRG	2 cut(s)	1063, 3155
BsiSI	CCGG	2 cut(s)	53, 85
BslFI	GGGAC	2 cut(s)	1029, 1212
BsmAI	GTCTC	3 cut(s)	448, 796, 2448
BsmFI	GGGAC	2 cut(s)	1029, 1212
BsmI	GAATGC	2 cut(s)	2735, 2856
Bso31I	GGTCTC	3 cut(s)	448, 796, 2448
BsoBI	CYCGRG	2 cut(s)	1063, 3155
Bsp119I	TTCGAA	1 cut(s)	754
Bsp1286I	GDGCHC	4 cut(s)	948, 2001, 2432, 3001
Bsp1720I	GCTNAGC	1 cut(s)	2535
Bsp68I	TCGCGA	1 cut(s)	106
BspCNI	CTCAG	5 cut(s)	1891, 2458, 2527, 2583, 3160
BspFNI	CGCG	3 cut(s)	106, 216, 2217
BspLI	GGNNCC	9 cut(s)	223, 843, 997, 1521, 1667, 2481, 2868, 3153, 3433
BspMAI	CTGCAG	3 cut(s)	2040, 2700, 3091
BspMI	ACCTGC	5 cut(s)	193, 765, 1153, 2302, 3094
BspOI	GCTAGC	1 cut(s)	1800
BspPI	GGATC	6 cut(s)	21, 86, 883, 1524, 3042, 3271
BspQI	GCTCTTC	1 cut(s)	2553
BspT104I	TTCGAA	1 cut(s)	754
BspT107I	GGYRCC	2 cut(s)	1665, 3431
BspTNI	GGTCTC	3 cut(s)	448, 796, 2448
BsrBI	CCGCTC	2 cut(s)	291, 3337
BsrDI	GCAATG	2 cut(s)	1571, 2231
BsrFI	RCCGGY	2 cut(s)	52, 84
BsrI	ACTGG	4 cut(s)	135, 1327, 1521, 1788
BssAI	RCCGGY	2 cut(s)	52, 84
BssNI	GRCGYC	2 cut(s)	88, 1666
BssSI	CACGAG	2 cut(s)	127, 879
BssT1I	CCWWGG	6 cut(s)	832, 952, 1001, 1289, 1365, 2523
Bst2BI	CACGAG	2 cut(s)	127, 879
Bst2UI	CCWGG	6 cut(s)	970, 1079, 1317, 1671, 1707, 2198
Bst4CI	ACNGT	6 cut(s)	344, 1251, 1745, 2393, 2983, 3135
Bst6I	CTCTTC	6 cut(s)	552, 705, 714, 814, 2323, 2553
BstACI	GRCGYC	2 cut(s)	88, 1666
BstAPI	GCANNNNNTGC	1 cut(s)	2879
BstBI	TTCGAA	1 cut(s)	754
BstDSI	CCRYGG	1 cut(s)	1603
BstEII	GGTNACC	1 cut(s)	1073
BstFNI	CGCG	3 cut(s)	106, 216, 2217
BstH2I	RGCGCY	1 cut(s)	1669
BstHHI	GCGC	4 cut(s)	216, 483, 1668, 2219
BstMAI	GTCTC	3 cut(s)	448, 796, 2448
BstMCI	CGRYCG	2 cut(s)	41, 3062
BstNI	CCWGG	6 cut(s)	970, 1079, 1317, 1671, 1707, 2198
BstNSI	RCATGY	5 cut(s)	115, 384, 1027, 2546, 2730
BstPI	GGTNACC	1 cut(s)	1073
BstSCI	CCNGG	6 cut(s)	968, 1077, 1315, 1669, 1705, 2196
BstSFI	CTRYAG	3 cut(s)	2036, 2696, 3087
BstSLI	GKGCMC	2 cut(s)	2001, 2432
BstUI	CGCG	3 cut(s)	106, 216, 2217
BstV2I	GAAGAC	6 cut(s)	366, 651, 745, 2376, 3111, 3276
BstX2I	RGATCY	6 cut(s)	13, 989, 1529, 2375, 3172, 3263
BstYI	RGATCY	6 cut(s)	13, 989, 1529, 2375, 3172, 3263
BstZI	CGGCCG	1 cut(s)	3059
Bsu36I	CCTNAGG	1 cut(s)	1878
BsuI	GTATCC	2 cut(s)	1263, 2347
BtgI	CCRYGG	1 cut(s)	1603
BtgZI	GCGATG	3 cut(s)	231, 651, 681
BtrI	CACGTC	1 cut(s)	3141
BtsI	GCAGTG	1 cut(s)	2100
BtsIMutI	CAGTG	4 cut(s)	1839, 2100, 2292, 2873
BtuMI	TCGCGA	1 cut(s)	106
BveI	ACCTGC	5 cut(s)	193, 765, 1153, 2302, 3094
CaiI	CAGNNNCTG	1 cut(s)	2459
CfoI	GCGC	4 cut(s)	216, 483, 1668, 2219
Cfr10I	RCCGGY	2 cut(s)	52, 84
Cfr13I	GGNCC	9 cut(s)	842, 956, 995, 1124, 1310, 1717, 2498, 2866, 2912
CseI	GACGC	2 cut(s)	77, 881
Csp6I	GTAC	5 cut(s)	132, 1320, 2843, 2969, 3131
CviQI	GTAC	5 cut(s)	132, 1320, 2843, 2969, 3131
DinI	GGCGCC	1 cut(s)	1667
DraI	TTTAAA	1 cut(s)	1827
EaeI	YGGCCR	3 cut(s)	1372, 3059, 3244
EagI	CGGCCG	1 cut(s)	3059
Eam1104I	CTCTTC	6 cut(s)	552, 705, 714, 814, 2323, 2553
EarI	CTCTTC	6 cut(s)	552, 705, 714, 814, 2323, 2553
EciI	GGCGGA	1 cut(s)	91
Ecl136II	GAGCTC	1 cut(s)	946
EclXI	CGGCCG	1 cut(s)	3059
Eco130I	CCWWGG	6 cut(s)	832, 952, 1001, 1289, 1365, 2523
Eco147I	AGGCCT	1 cut(s)	2161
Eco24I	GRGCYC	1 cut(s)	948
Eco31I	GGTCTC	3 cut(s)	448, 796, 2448
Eco47I	GGWCC	5 cut(s)	995, 1310, 2498, 2866, 2912
Eco52I	CGGCCG	1 cut(s)	3059
Eco53kI	GAGCTC	1 cut(s)	946
Eco57I	CTGAAG	6 cut(s)	1863, 2319, 2388, 2949, 3246, 3288
Eco81I	CCTNAGG	1 cut(s)	1878
Eco88I	CYCGRG	2 cut(s)	1063, 3155
Eco91I	GGTNACC	1 cut(s)	1073
EcoICRI	GAGCTC	1 cut(s)	946
EcoO109I	RGGNCCY	1 cut(s)	956
EcoO65I	GGTNACC	1 cut(s)	1073
EcoRI	GAATTC	3 cut(s)	1110, 1688, 2589
EcoRII	CCWGG	6 cut(s)	968, 1077, 1315, 1669, 1705, 2196
EcoT14I	CCWWGG	6 cut(s)	832, 952, 1001, 1289, 1365, 2523
EcoT22I	ATGCAT	3 cut(s)	30, 337, 1029
EcoT38I	GRGCYC	1 cut(s)	948
EgeI	GGCGCC	1 cut(s)	1667
EheI	GGCGCC	1 cut(s)	1667
ErhI	CCWWGG	6 cut(s)	832, 952, 1001, 1289, 1365, 2523
FalI	AAGNNNNNCTT	2 cut(s)	1928, 1960
FaqI	GGGAC	2 cut(s)	1029, 1212
FauI	CCCGC	2 cut(s)	65, 293
FblI	GTMKAC	2 cut(s)	1568, 1884
FriOI	GRGCYC	1 cut(s)	948
FspBI	CTAG	6 cut(s)	1019, 1797, 2004, 2237, 2805, 2988
GlaI	GCGC	4 cut(s)	215, 482, 1667, 2218
GsaI	CCCAGC	3 cut(s)	1125, 1976, 2978
GsuI	CTGGAG	3 cut(s)	178, 1805, 3041
HaeII	RGCGCY	1 cut(s)	1669
HapII	CCGG	2 cut(s)	53, 85
HgaI	GACGC	2 cut(s)	77, 881
HhaI	GCGC	4 cut(s)	216, 483, 1668, 2219
Hin1I	GRCGYC	2 cut(s)	88, 1666
Hin6I	GCGC	4 cut(s)	214, 481, 1666, 2217
HinP1I	GCGC	4 cut(s)	214, 481, 1666, 2217
HincII	GTYRAC	3 cut(s)	568, 1962, 2707
HindII	GTYRAC	3 cut(s)	568, 1962, 2707
HindIII	AAGCTT	1 cut(s)	2114
HpaII	CCGG	2 cut(s)	53, 85
Hpy166II	GTNNAC	8 cut(s)	568, 1569, 1607, 1885, 1962, 1999, 2707, 3138
Hpy8I	GTNNAC	8 cut(s)	568, 1569, 1607, 1885, 1962, 1999, 2707, 3138
Hpy99I	CGWCG	1 cut(s)	492
HpyCH4III	ACNGT	6 cut(s)	344, 1251, 1745, 2393, 2983, 3135
HpyCH4IV	ACGT	4 cut(s)	742, 1331, 2335, 3140
HpySE526I	ACGT	4 cut(s)	742, 1331, 2335, 3140
Hsp92I	GRCGYC	2 cut(s)	88, 1666
HspAI	GCGC	4 cut(s)	214, 481, 1666, 2217
KasI	GGCGCC	1 cut(s)	1665
KroI	GCCGGC	2 cut(s)	52, 84
KroNI	GCCGGC	2 cut(s)	54, 86
LguI	GCTCTTC	1 cut(s)	2553
MaeI	CTAG	6 cut(s)	1019, 1797, 2004, 2237, 2805, 2988
MaeII	ACGT	4 cut(s)	742, 1331, 2335, 3140
MaeIII	GTNAC	5 cut(s)	1073, 2856, 2888, 2983, 3111
MbiI	CCGCTC	2 cut(s)	291, 3337
MfeI	CAATTG	2 cut(s)	1445, 1647
MflI	RGATCY	6 cut(s)	13, 989, 1529, 2375, 3172, 3263
MhlI	GDGCHC	4 cut(s)	948, 2001, 2432, 3001
MlsI	TGGCCA	2 cut(s)	1374, 3246
MluNI	TGGCCA	2 cut(s)	1374, 3246
Mly113I	GGCGCC	1 cut(s)	1666
MlyI	GAGTC	3 cut(s)	920, 2702, 3320
MmeI	TCCRAC	3 cut(s)	1727, 1786, 2403
Mox20I	TGGCCA	2 cut(s)	1374, 3246
Mph1103I	ATGCAT	3 cut(s)	30, 337, 1029
MreI	CGCCGGCG	1 cut(s)	52
MroNI	GCCGGC	2 cut(s)	52, 84
MroXI	GAANNNNTTC	1 cut(s)	2244
MscI	TGGCCA	2 cut(s)	1374, 3246
MseI	TTAA	5 cut(s)	1334, 1778, 1826, 2516, 3443
MslI	CAYNNNNRTG	4 cut(s)	1080, 1986, 3252, 3278
Msp20I	TGGCCA	2 cut(s)	1374, 3246
MspA1I	CMGCKG	4 cut(s)	72, 1976, 2974, 3019
MspI	CCGG	2 cut(s)	53, 85
MspR9I	CCNGG	6 cut(s)	970, 1079, 1317, 1671, 1707, 2198
MunI	CAATTG	2 cut(s)	1445, 1647
Mva1269I	GAATGC	2 cut(s)	2735, 2856
MvaI	CCWGG	6 cut(s)	970, 1079, 1317, 1671, 1707, 2198
MvnI	CGCG	3 cut(s)	106, 216, 2217
NaeI	GCCGGC	2 cut(s)	54, 86
NarI	GGCGCC	1 cut(s)	1666
NgoMIV	GCCGGC	2 cut(s)	52, 84
NheI	GCTAGC	1 cut(s)	1796
NlaIV	GGNNCC	9 cut(s)	223, 843, 997, 1521, 1667, 2481, 2868, 3153, 3433
NmeAIII	GCCGAG	2 cut(s)	445, 2143
NmuCI	GTSAC	5 cut(s)	1073, 2856, 2888, 2983, 3111
NruI	TCGCGA	1 cut(s)	106
NsiI	ATGCAT	3 cut(s)	30, 337, 1029
NspI	RCATGY	5 cut(s)	115, 384, 1027, 2546, 2730
NspV	TTCGAA	1 cut(s)	754
OliI	CACNNNNGTG	2 cut(s)	1080, 1986
PaeI	GCATGC	1 cut(s)	2546
PaqCI	CACCTGC	2 cut(s)	2302, 3094
PasI	CCCWGGG	1 cut(s)	969
PceI	AGGCCT	1 cut(s)	2161
PciI	ACATGT	2 cut(s)	380, 2726
PciSI	GCTCTTC	1 cut(s)	2553
PcsI	WCGNNNNNNNCGW	3 cut(s)	714, 814, 1861
PctI	GAATGC	2 cut(s)	2735, 2856
PdiI	GCCGGC	2 cut(s)	54, 86
PdmI	GAANNNNTTC	1 cut(s)	2244
PfeI	GAWTC	8 cut(s)	145, 322, 397, 900, 917, 1598, 1919, 2450
PflMI	CCANNNNNTGG	1 cut(s)	838
Ple19I	CGATCG	1 cut(s)	41
PleI	GAGTC	3 cut(s)	920, 2702, 3319
PluTI	GGCGCC	1 cut(s)	1669
PpsI	GAGTC	3 cut(s)	920, 2702, 3319
PscI	ACATGT	2 cut(s)	380, 2726
PsiI	TTATAA	1 cut(s)	2781
Psp124BI	GAGCTC	1 cut(s)	948
Psp1406I	AACGTT	2 cut(s)	1331, 2335
Psp6I	CCWGG	6 cut(s)	968, 1077, 1315, 1669, 1705, 2196
PspEI	GGTNACC	1 cut(s)	1073
PspFI	CCCAGC	3 cut(s)	1121, 1972, 2974
PspGI	CCWGG	6 cut(s)	968, 1077, 1315, 1669, 1705, 2196
PspN4I	GGNNCC	9 cut(s)	223, 843, 997, 1521, 1667, 2481, 2868, 3153, 3433
PspPI	GGNCC	9 cut(s)	842, 956, 995, 1124, 1310, 1717, 2498, 2866, 2912
PstI	CTGCAG	3 cut(s)	2040, 2700, 3091
PstNI	CAGNNNCTG	1 cut(s)	2459
PsuI	RGATCY	6 cut(s)	13, 989, 1529, 2375, 3172, 3263
PvuI	CGATCG	1 cut(s)	41
PvuII	CAGCTG	3 cut(s)	1976, 2974, 3019
RruI	TCGCGA	1 cut(s)	106
RsaI	GTAC	5 cut(s)	133, 1321, 2844, 2970, 3132
RsaNI	GTAC	5 cut(s)	132, 1320, 2843, 2969, 3131
RseI	CAYNNNNRTG	4 cut(s)	1080, 1986, 3252, 3278
SacI	GAGCTC	1 cut(s)	948
SapI	GCTCTTC	1 cut(s)	2553
SaqAI	TTAA	5 cut(s)	1334, 1778, 1826, 2516, 3443
Sau96I	GGNCC	9 cut(s)	842, 956, 995, 1124, 1310, 1717, 2498, 2866, 2912
SchI	GAGTC	3 cut(s)	920, 2702, 3320
ScrFI	CCNGG	6 cut(s)	970, 1079, 1317, 1671, 1707, 2198
SduI	GDGCHC	4 cut(s)	948, 2001, 2432, 3001
SfcI	CTRYAG	3 cut(s)	2036, 2696, 3087
SfoI	GGCGCC	1 cut(s)	1667
SfuI	TTCGAA	1 cut(s)	754
SgrAI	CRCCGGYG	1 cut(s)	52
SinI	GGWCC	5 cut(s)	995, 1310, 2498, 2866, 2912
SmiMI	CAYNNNNRTG	4 cut(s)	1080, 1986, 3252, 3278
SmlI	CTYRAG	2 cut(s)	2904, 3365
SmoI	CTYRAG	2 cut(s)	2904, 3365
SphI	GCATGC	1 cut(s)	2546
SseBI	AGGCCT	1 cut(s)	2161
SspDI	GGCGCC	1 cut(s)	1665
SspI	AATATT	2 cut(s)	2325, 3073
SspMI	CTAG	6 cut(s)	1019, 1797, 2004, 2237, 2805, 2988
SstI	GAGCTC	1 cut(s)	948
StuI	AGGCCT	1 cut(s)	2161
StyD4I	CCNGG	6 cut(s)	968, 1077, 1315, 1669, 1705, 2196
StyI	CCWWGG	6 cut(s)	832, 952, 1001, 1289, 1365, 2523
TaaI	ACNGT	6 cut(s)	344, 1251, 1745, 2393, 2983, 3135
TaiI	ACGT	4 cut(s)	745, 1334, 2338, 3143
TaqI	TCGA	7 cut(s)	754, 817, 898, 948, 2833, 3001, 3402
TatI	WGTACW	1 cut(s)	2842
TauI	GCSGC	6 cut(s)	59, 191, 291, 1666, 3083, 3325
TfiI	GAWTC	8 cut(s)	145, 322, 397, 900, 917, 1598, 1919, 2450
Tru1I	TTAA	5 cut(s)	1334, 1778, 1826, 2516, 3443
Tru9I	TTAA	5 cut(s)	1334, 1778, 1826, 2516, 3443
TscAI	CASTG	4 cut(s)	1846, 2100, 2292, 2880
TseFI	GTSAC	5 cut(s)	1073, 2856, 2888, 2983, 3111
Tsp45I	GTSAC	5 cut(s)	1073, 2856, 2888, 2983, 3111
TspGWI	ACGGA	2 cut(s)	79, 1853
TspRI	CASTG	4 cut(s)	1846, 2100, 2292, 2880
Van91I	CCANNNNNTGG	1 cut(s)	838
VneI	GTGCAC	1 cut(s)	1997
VpaK11BI	GGWCC	5 cut(s)	995, 1310, 2498, 2866, 2912
XapI	RAATTY	9 cut(s)	1110, 1548, 1688, 1728, 1901, 2243, 2589, 3464, 3484
XceI	RCATGY	5 cut(s)	115, 384, 1027, 2546, 2730
XmiI	GTMKAC	2 cut(s)	1568, 1884
XmnI	GAANNNNTTC	1 cut(s)	2244
XspI	CTAG	6 cut(s)	1019, 1797, 2004, 2237, 2805, 2988
Zsp2I	ATGCAT	3 cut(s)	30, 337, 1029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.