Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0004176

Physical Location & Seq

Genomic Coordinates Reverse (-)

36234576 .. 36241214

Gene Structure 6639 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000008748

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3645 bp

ATGCATCCGAAGCGATCGGATGGGTCGCCGGCGGCATTACGGAGCAGCGGGCGGATCAGCCGGCGTCCGAAAACTTACTCGCGAAGCATGTTCTATTACAACACGAGTACCAGTAGCATGATTCACAAACGCAGAAGGAAGGCCAAGACCAAGAACCGACCTGCCGCCTCCAGAATTGCTAAGATGATGCGCGATGGCTCCACTCGTTCCCAAAGATCGCCCCACCCCACTTCTCCCACTAAGCTCAATACAAACTTACCCCGCCGCTCCGAAAGAAGGAGAACTCTGTCTGTAAAGAATCGAGATTATGCATCAGACAGTTCCGATGGTGATGAAGACATGATGAAACCCTCTACATGTAAATCTATCAAGAATCGGGGTGCATATCAAGATGAATTATCACCTTCCAAGCACAAAAAGATGGTGGAGAGACCAACACCTCGGCGTGAAGGATTGCGCCCTCGTCGCTCTAAGACTATTTCAAATGAGGATATACTTTTTGGATATGACGATCTTCCTGTTGATAGTTCGGAAGAGAAGGTTGACCAAGAAGAAACCGAAAATGGGCAGGATATTGAATACAATGATGCAGATGATGGTCAGAATGAGGGCGATGGTGAAGACATGGGTGATGATGATGGAGATGAAGATGGTGATGATGATGGTGATGACGAAGAGGGTGAAGAGGAACAAGATGGAAGAAGACGTTATGATCTTCGAAATCGTGCAGAAGTCCGCAGGTTCTCTATTGAAGAAGGTAAGCGGAGACCACGATCTCCTCGAAGAGTATTGCACCAAGGAATGGGGCCAAAAGTCAGTAGGGATGTAAGAAAGGGTGGATCACGAGTTCATAAGCGTCATCAAATCACAAGGACTGATGATTCTGATGACTCCCTTCTTGTGGATGAGCTCGACCAGGGCCCTGCAATTCCTTGGGGGAAAGGTGGGAGCAGATCTGGACCACCTTGGCTTTTTGGGGGACTAGACATGCATGGAACAACGACATGGGGTTTGAATGTTGCTGCCTCGGGTTGGGGTCACCAGGGTGATGCTTTTGCTACCTTGACTTCTGGAATTCAAACTGCTGGGCCAAGCTCAAAGGGAGGGGCAGACATACAACCTTTGCAGGTTGATGATAGTGTCAGTTTTGAGGATATAGGGGGACTTTCTCAATATATTGATGCTTTGAAGGAAATGGTTTTCTTTCCTTTACTGTACCCAGATTTCTTTGCGAGCTATCATATCACCCCACCAAGGGGAGTTTTGTTATGTGGTCCTCCTGGTACTGGGAAAACGTTAATTGCCAGAGCCTTAGCCTCTGCTGCATCCAAGGCTGGCCAGAAAGTGAGTTTTTACATGAGGAAGGGTGCAGATGTCCTAAGCAAGTGGGTTGGCGAGGCTGAAAGACAATTGAAACTTCTTTTTGAGGAAGCACAAAGAAATCAACCTTCAATCATATTCTTTGATGAAATAGATGGACTGGCTCCTGTAAGATCCAGCAAACAAGAGCAAATTCATAATTCTATTGTGTCTACATTGCTTGCTCTGATGGATGGTCTTGATTCCCGTGGACAAGTGGTTTTGATCGGAGCAACCAACCGAATTGATGCAATTGATGGAGCTTTGCGGCGCCCTGGTAGATTTGATCGTGAATTCAACTTTCCTTTGCCTGGCTGTGAGGCCCGTGCTGAAATTTTAGACATTCACAGCCGGAAATGGAAGCATCCTCCTTCAAATGAGTTAAAACTGGAGCTTGCAGCTAGCTGTGTTGGATATTGTGGTGCTGATTTAAAGGCTCTTTGCACTGAAGCTGCTATTCGTGCTTTCCGTGAAAAATATCCTCAGGTCTACACAAGTGATGATAAATTTGTAATTGATGTTGATTCAGTAAGGGTTGAAAAGTATCACTTTGTTGAAGCCATGTCAACAATCACCCCAGCTGCTCACAGAGGTGCTGTTGTGCACTCTAGGCCATTGTCTTTAGTAGTTGCACCATGCCTGCAGAGGCATCTCCAAAGAGCCATGAACTATATATCCGATATTTTCCCTCCGATTGCAGTGTCATCAGAATTGACCAAGCTTTCCATGCTCTCTTGTGGCTCTGCCATTCCTCTTGTTTATAGGCCTCGGCTTCTTCTATGTGGTGGTGAAGGTTCTGGCCTGGATCATCTTGGGCCTGCTATTTTACATGAACTCGAGAAGTTTCCTGTCCATTCTCTAGGACTTCCATCTCTTCTATCTGATCCTAGTGCAAAGACACCAGAGGAAGCACTGGTACATATATTTGGTGAAGCAAGGAGGACGACGCCATCAATTCTCTACTTGCCGCAGTTCAATTTGTGGTGGGAGACGGCACATGAGCAGCTCCGCGCAGTTTTGCTGACATTGCTAGAAGAATTTCCATCTGATTTACCTATATTACTACTTGCAACGTCATCAGTGCCACCTGCTGAAGTTGATGCTATGACCTCTTCAATATTCTCCGAACGTTCTGTCTATCAGGTGGATACGCCTTCTACTGAAGACAGATCTTTGTTTTTTGACCGTCTGATTGAAGCTGCCTTGTCAGTCTTGTTGGAGGGCACGACTAAAAGGTCTCAGGAATCAGTATCTGTTCCTGAACTTCCAAAGGCTCCAAAAGTGGCAAGCGGTCCAAAGGTGTCAGAGTTAAAAGCCAAGGTAGAAGCTGAGCAGCATGCTCTTCGCCGATTGCGGATGTGCCTCAGAGATGTTTGCAATAGAATTCTATACGATAAGAGGTTTAGTGCTTTTCACTATCCTGTCTTGGATGAGGATGCTCCAAACTATCGCTCAATTATCCAAAATCCTATGGATGTTGCAACTCTGCTGCAGCGTGTTGACTCTGGGCTGTATATTACATGTTCAGCATTCCTGCAAGATGTTGATCTTATAGTTTCTAATGCAAAGGCTTATAATGGAGATGATTACAATGGTGCTAGGATTGTTAGTAGAGGTTATGAGCTTCGAGATGCCGTACATGGAATGCTGTCACAGATGGACCCTGCACTGATTGCATATTGTGACAAGATTGCTGCTCAAGGTGGTCCAGAACAAATACCTGAAGATTTAGGGGTAGCTACTTTCCCATCAATGCCTGTTGTACAGCTGGGAACTGTCACTAGGGCGAGTGCTCGACTTCGTAATGTGCAGCCGGAGGTAAATCTGGATCATAGCTACGAGGCACACAAACGGCCGAAGAAGAATATTGATGCCGCACCTGCAGCTTCAACAGCAGAAGACAAGTCACAGCATCAAGGTTCTGTACCGTCCACGTCATCTCAGGAACCCGAGACGAATAACACAGATCTTGGAGTGCCGGAAACCTCTTCTGTTGTTGCCAATCAGCTTGAAACTTCTGAAGTAGTAGAAGTTTCTGGCCATGCTGATGCGAGTGGATCTGAAGACATCAAGATGTTAGATGGTGAGATTACAAACCAAATGGAGTCTGTCAAGCGGCTTTTTGTGGAGCGGACCAAAACTTATGACATTCCACAGCTTGAGAGGCTTTATGCAAGAATTATGAAGGGCATTTTCGACATCAAGGACAAAAGCGATATAGATGGCACCAAGCATTTAATTTTGAAGTATTTGTTGAAATTTGCGGAGGATGAAGCAAATTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003682 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010639 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031935 GO:0031936 GO:0033043 GO:0033044 GO:0034641 GO:0035510 GO:0040029 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045815 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0080090 GO:0080111 GO:0090304 GO:1901360 GO:1902275 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:2000112 GO:2001141 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Bromodomain

Protein Analysis

1215

Amino Acids

134.61

Weight (kDa)

5.92

Isoelectric Point (pI)

58.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IstB_IS21	PF01695	419 - 503	3.6e-07	IstB-like ATP binding protein
AAA	PF00004	421 - 556	4.8e-39	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	584 - 620	1.8e-10	AAA+ lid domain
Bromodomain	PF00439	912 - 982	2e-13	Bromodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004176)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05910
fragaria_vesca	FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720 FvH4_4g10720
malus_domestica	MD04G1007800.v1.1
prunus_persica	Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1 Prupe.1G191800_v2.0.a1
pyrus_communis	pycom04g03460
rosa_chinensis	RchiOBHm_Chr3g0496841 RchiOBHm_Chr4g0406861
rosa_laevigata	RLG00000008748 RLG00000022493
rosa_multiflora	Rmu_sc0000600.1_g000031 Rmu_sc0001438.1_g000025
rosa_roxburghii	Rroxscaffold_5G00351650 Rroxscaffold_6G00388620
rosa_rugosa	Rorug03G0288300 Rorug04G0075300
rosa_samantha	Rh3AG332000 Rh3BG368600 Rh3CG364900 Rh3DG368000 Rh4AG141500 Rh4BG138300 Rh4CG148200 Rh4DG135400
rosa_wichuraiana	Rw3G029090 Rw4G011520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2934
AarI	CACCTGC	2 cut(s)	2455, 3247
AasI	GACNNNNNNGTC	1 cut(s)	2593
Acc36I	ACCTGC	5 cut(s)	169, 729, 1117, 2455, 3247
AccB1I	GGYRCC	2 cut(s)	1629, 3584
AccB7I	CCANNNNNTGG	1 cut(s)	802
AccBSI	CCGCTC	2 cut(s)	267, 3490
AccI	GTMKAC	2 cut(s)	1532, 1848
AccII	CGCG	3 cut(s)	82, 192, 2370
AclI	AACGTT	2 cut(s)	1295, 2488
AclWI	GGATC	7 cut(s)	62, 847, 1488, 2172, 2237, 3195, 3424
AcoI	YGGCCR	3 cut(s)	1336, 3212, 3397
AcsI	RAATTY	9 cut(s)	1074, 1512, 1652, 1692, 1865, 2396, 2742, 3617, 3637
AcuI	CTGAAG	6 cut(s)	1827, 2472, 2541, 3102, 3399, 3441
AcyI	GRCGYC	3 cut(s)	64, 1630, 2306
AfaI	GTAC	7 cut(s)	109, 1217, 1285, 2277, 2997, 3123, 3285
AflIII	ACRYGT	2 cut(s)	356, 2879
AjiI	CACGTC	1 cut(s)	3294
AjnI	CCWGG	6 cut(s)	915, 1041, 1279, 1633, 1669, 2160
AleI	CACNNNNGTG	2 cut(s)	1044, 1950
AloI	GAACNNNNNNTCC	4 cut(s)	831, 863, 2018, 2050
Alw21I	GWGCWC	3 cut(s)	912, 1965, 3154
Alw26I	GTCTC	5 cut(s)	424, 760, 2342, 2601, 3305
Alw44I	GTGCAC	1 cut(s)	1961
AlwI	GGATC	7 cut(s)	62, 847, 1488, 2172, 2237, 3195, 3424
AlwNI	CAGNNNCTG	1 cut(s)	2612
Ama87I	CYCGRG	3 cut(s)	1027, 2195, 3308
ApaI	GGGCCC	1 cut(s)	923
ApaLI	GTGCAC	1 cut(s)	1961
ApoI	RAATTY	9 cut(s)	1074, 1512, 1652, 1692, 1865, 2396, 2742, 3617, 3637
ArsI	GACNNNNNNTTYG	4 cut(s)	994, 1026, 2478, 2510
Asp700I	GAANNNNTTC	1 cut(s)	2397
AspLEI	GCGC	4 cut(s)	192, 459, 1632, 2372
AsuII	TTCGAA	1 cut(s)	718
AsuNHI	GCTAGC	1 cut(s)	1760
AvaI	CYCGRG	3 cut(s)	1027, 2195, 3308
AvaII	GGWCC	6 cut(s)	959, 1274, 2651, 3019, 3065, 3492
AxyI	CCTNAGG	1 cut(s)	1842
BaeGI	GKGCMC	3 cut(s)	923, 1965, 2585
BaeI	ACNNNNGTAYC	2 cut(s)	91, 124
BalI	TGGCCA	2 cut(s)	1338, 3399
BanI	GGYRCC	2 cut(s)	1629, 3584
BanII	GRGCYC	2 cut(s)	912, 923
BarI	GAAGNNNNNNTAC	2 cut(s)	2259, 2291
BauI	CACGAG	2 cut(s)	103, 843
BbsI	GAAGAC	6 cut(s)	342, 627, 709, 2529, 3264, 3429
Bbv12I	GWGCWC	3 cut(s)	912, 1965, 3154
BceAI	ACGGC	3 cut(s)	2367, 2978, 3227
BcgI	CGANNNNNNTGC	2 cut(s)	2684, 2718
BciT130I	CCWGG	6 cut(s)	917, 1043, 1281, 1635, 1671, 2162
BciVI	GTATCC	1 cut(s)	2500
BcoDI	GTCTC	5 cut(s)	424, 760, 2342, 2601, 3305
BfaI	CTAG	8 cut(s)	983, 1761, 1968, 2219, 2247, 2390, 2958, 3141
BfmI	CTRYAG	3 cut(s)	2000, 2849, 3240
BfoI	RGCGCY	1 cut(s)	1633
BfuAI	ACCTGC	5 cut(s)	169, 729, 1117, 2455, 3247
BfuI	GTATCC	1 cut(s)	2500
BglII	AGATCT	3 cut(s)	953, 2528, 3325
BlpI	GCTNAGC	1 cut(s)	2688
Bme1390I	CCNGG	6 cut(s)	917, 1043, 1281, 1635, 1671, 2162
Bme18I	GGWCC	6 cut(s)	959, 1274, 2651, 3019, 3065, 3492
BmeT110I	CYCGRG	3 cut(s)	1027, 2195, 3308
BmgBI	CACGTC	1 cut(s)	3294
BmiI	GGNNCC	9 cut(s)	199, 807, 921, 1485, 1631, 2634, 3021, 3306, 3586
BmrFI	CCNGG	6 cut(s)	917, 1043, 1281, 1635, 1671, 2162
BmrI	ACTGGG	1 cut(s)	1296
BmtI	GCTAGC	1 cut(s)	1764
BmuI	ACTGGG	1 cut(s)	1296
BpiI	GAAGAC	6 cut(s)	342, 627, 709, 2529, 3264, 3429
BpmI	CTGGAG	2 cut(s)	154, 1769
Bpu10I	CCTNAGC	2 cut(s)	1312, 1379
Bpu1102I	GCTNAGC	1 cut(s)	2688
Bpu14I	TTCGAA	1 cut(s)	718
BpuEI	CTTGAG	2 cut(s)	3042, 3539
BsaBI	GATNNNNATC	1 cut(s)	2904
BsaHI	GRCGYC	3 cut(s)	64, 1630, 2306
BsaI	GGTCTC	3 cut(s)	424, 760, 2601
Bse118I	RCCGGY	2 cut(s)	28, 60
Bse1I	ACTGG	5 cut(s)	111, 1291, 1485, 1752, 2277
Bse21I	CCTNAGG	1 cut(s)	1842
Bse3DI	GCAATG	2 cut(s)	1535, 2384
Bse8I	GATNNNNATC	1 cut(s)	2904
BseBI	CCWGG	6 cut(s)	917, 1043, 1281, 1635, 1671, 2162
BseJI	GATNNNNATC	1 cut(s)	2904
BseMI	GCAATG	2 cut(s)	1535, 2384
BseMII	CTCAG	5 cut(s)	1856, 2612, 2679, 2737, 3314
BseNI	ACTGG	5 cut(s)	111, 1291, 1485, 1752, 2277
BseRI	GAGGAG	1 cut(s)	768
BseSI	GKGCMC	3 cut(s)	923, 1965, 2585
BseX3I	CGGCCG	1 cut(s)	3212
BseYI	CCCAGC	3 cut(s)	1085, 1936, 3127
BsgI	GTGCAG	4 cut(s)	747, 1389, 3009, 3188
Bsh1236I	CGCG	3 cut(s)	82, 192, 2370
Bsh1285I	CGRYCG	2 cut(s)	17, 3215
BshNI	GGYRCC	2 cut(s)	1629, 3584
BsiEI	CGRYCG	2 cut(s)	17, 3215
BsiHKAI	GWGCWC	3 cut(s)	912, 1965, 3154
BsiHKCI	CYCGRG	3 cut(s)	1027, 2195, 3308
BsiSI	CCGG	5 cut(s)	29, 61, 1711, 3173, 3338
BslFI	GGGAC	2 cut(s)	993, 1176
BsmAI	GTCTC	5 cut(s)	424, 760, 2342, 2601, 3305
BsmBI	CGTCTC	2 cut(s)	2342, 3305
BsmFI	GGGAC	2 cut(s)	993, 1176
BsmI	GAATGC	2 cut(s)	2888, 3009
Bso31I	GGTCTC	3 cut(s)	424, 760, 2601
BsoBI	CYCGRG	3 cut(s)	1027, 2195, 3308
Bsp119I	TTCGAA	1 cut(s)	718
Bsp120I	GGGCCC	1 cut(s)	919
Bsp1286I	GDGCHC	5 cut(s)	912, 923, 1965, 2585, 3154
Bsp1407I	TGTACA	1 cut(s)	3121
Bsp1720I	GCTNAGC	1 cut(s)	2688
Bsp68I	TCGCGA	1 cut(s)	82
BspCNI	CTCAG	5 cut(s)	1855, 2611, 2680, 2736, 3313
BspFNI	CGCG	3 cut(s)	82, 192, 2370
BspLI	GGNNCC	9 cut(s)	199, 807, 921, 1485, 1631, 2634, 3021, 3306, 3586
BspMAI	CTGCAG	3 cut(s)	2004, 2853, 3244
BspMI	ACCTGC	5 cut(s)	169, 729, 1117, 2455, 3247
BspOI	GCTAGC	1 cut(s)	1764
BspPI	GGATC	7 cut(s)	62, 847, 1488, 2172, 2237, 3195, 3424
BspQI	GCTCTTC	1 cut(s)	2706
BspT104I	TTCGAA	1 cut(s)	718
BspT107I	GGYRCC	2 cut(s)	1629, 3584
BspTNI	GGTCTC	3 cut(s)	424, 760, 2601
BsrBI	CCGCTC	2 cut(s)	267, 3490
BsrDI	GCAATG	2 cut(s)	1535, 2384
BsrFI	RCCGGY	2 cut(s)	28, 60
BsrGI	TGTACA	1 cut(s)	3121
BsrI	ACTGG	5 cut(s)	111, 1291, 1485, 1752, 2277
BssAI	RCCGGY	2 cut(s)	28, 60
BssNI	GRCGYC	3 cut(s)	64, 1630, 2306
BssSI	CACGAG	2 cut(s)	103, 843
BssT1I	CCWWGG	6 cut(s)	796, 932, 965, 1253, 1329, 2676
Bst2BI	CACGAG	2 cut(s)	103, 843
Bst2UI	CCWGG	6 cut(s)	917, 1043, 1281, 1635, 1671, 2162
Bst4CI	ACNGT	5 cut(s)	320, 1215, 2546, 3136, 3288
Bst6I	CTCTTC	8 cut(s)	528, 669, 678, 778, 2238, 2476, 2706, 3352
BstACI	GRCGYC	3 cut(s)	64, 1630, 2306
BstAPI	GCANNNNNTGC	1 cut(s)	3032
BstAUI	TGTACA	1 cut(s)	3121
BstBI	TTCGAA	1 cut(s)	718
BstDSI	CCRYGG	1 cut(s)	1567
BstEII	GGTNACC	1 cut(s)	1037
BstFNI	CGCG	3 cut(s)	82, 192, 2370
BstH2I	RGCGCY	1 cut(s)	1633
BstHHI	GCGC	4 cut(s)	192, 459, 1632, 2372
BstMAI	GTCTC	5 cut(s)	424, 760, 2342, 2601, 3305
BstMCI	CGRYCG	2 cut(s)	17, 3215
BstNI	CCWGG	6 cut(s)	917, 1043, 1281, 1635, 1671, 2162
BstNSI	RCATGY	5 cut(s)	91, 360, 991, 2699, 2883
BstPI	GGTNACC	1 cut(s)	1037
BstSCI	CCNGG	6 cut(s)	915, 1041, 1279, 1633, 1669, 2160
BstSFI	CTRYAG	3 cut(s)	2000, 2849, 3240
BstSLI	GKGCMC	3 cut(s)	923, 1965, 2585
BstUI	CGCG	3 cut(s)	82, 192, 2370
BstV2I	GAAGAC	6 cut(s)	342, 627, 709, 2529, 3264, 3429
BstX2I	RGATCY	5 cut(s)	953, 1493, 2528, 3325, 3416
BstYI	RGATCY	5 cut(s)	953, 1493, 2528, 3325, 3416
BstZI	CGGCCG	1 cut(s)	3212
Bsu36I	CCTNAGG	1 cut(s)	1842
BsuI	GTATCC	1 cut(s)	2500
BtgI	CCRYGG	1 cut(s)	1567
BtgZI	GCGATG	2 cut(s)	207, 627
BtrI	CACGTC	1 cut(s)	3294
BtsI	GCAGTG	1 cut(s)	2064
BtsIMutI	CAGTG	5 cut(s)	1803, 2064, 2270, 2445, 3026
BtuMI	TCGCGA	1 cut(s)	82
BveI	ACCTGC	5 cut(s)	169, 729, 1117, 2455, 3247
CaiI	CAGNNNCTG	1 cut(s)	2612
CfoI	GCGC	4 cut(s)	192, 459, 1632, 2372
Cfr10I	RCCGGY	2 cut(s)	28, 60
CseI	GACGC	3 cut(s)	53, 845, 2314
Csp6I	GTAC	7 cut(s)	108, 1216, 1284, 2276, 2996, 3122, 3284
CviQI	GTAC	7 cut(s)	108, 1216, 1284, 2276, 2996, 3122, 3284
DinI	GGCGCC	1 cut(s)	1631
DraI	TTTAAA	1 cut(s)	1791
DrdI	GACNNNNNNGTC	1 cut(s)	2593
DseDI	GACNNNNNNGTC	1 cut(s)	2593
EaeI	YGGCCR	3 cut(s)	1336, 3212, 3397
EagI	CGGCCG	1 cut(s)	3212
Eam1104I	CTCTTC	8 cut(s)	528, 669, 678, 778, 2238, 2476, 2706, 3352
EarI	CTCTTC	8 cut(s)	528, 669, 678, 778, 2238, 2476, 2706, 3352
EciI	GGCGGA	1 cut(s)	67
Ecl136II	GAGCTC	1 cut(s)	910
EclXI	CGGCCG	1 cut(s)	3212
Eco130I	CCWWGG	6 cut(s)	796, 932, 965, 1253, 1329, 2676
Eco147I	AGGCCT	1 cut(s)	2125
Eco24I	GRGCYC	2 cut(s)	912, 923
Eco31I	GGTCTC	3 cut(s)	424, 760, 2601
Eco47I	GGWCC	6 cut(s)	959, 1274, 2651, 3019, 3065, 3492
Eco52I	CGGCCG	1 cut(s)	3212
Eco53kI	GAGCTC	1 cut(s)	910
Eco57I	CTGAAG	6 cut(s)	1827, 2472, 2541, 3102, 3399, 3441
Eco81I	CCTNAGG	1 cut(s)	1842
Eco88I	CYCGRG	3 cut(s)	1027, 2195, 3308
Eco91I	GGTNACC	1 cut(s)	1037
EcoICRI	GAGCTC	1 cut(s)	910
EcoO109I	RGGNCCY	2 cut(s)	919, 920
EcoO65I	GGTNACC	1 cut(s)	1037
EcoRI	GAATTC	3 cut(s)	1074, 1652, 2742
EcoRII	CCWGG	6 cut(s)	915, 1041, 1279, 1633, 1669, 2160
EcoT14I	CCWWGG	6 cut(s)	796, 932, 965, 1253, 1329, 2676
EcoT22I	ATGCAT	3 cut(s)	6, 313, 993
EcoT38I	GRGCYC	2 cut(s)	912, 923
EgeI	GGCGCC	1 cut(s)	1631
EheI	GGCGCC	1 cut(s)	1631
ErhI	CCWWGG	6 cut(s)	796, 932, 965, 1253, 1329, 2676
Esp3I	CGTCTC	2 cut(s)	2342, 3305
FalI	AAGNNNNNCTT	2 cut(s)	1892, 1924
FaqI	GGGAC	2 cut(s)	993, 1176
FauI	CCCGC	2 cut(s)	41, 269
FblI	GTMKAC	2 cut(s)	1532, 1848
FriOI	GRGCYC	2 cut(s)	912, 923
FspBI	CTAG	8 cut(s)	983, 1761, 1968, 2219, 2247, 2390, 2958, 3141
GlaI	GCGC	4 cut(s)	191, 458, 1631, 2371
GsaI	CCCAGC	3 cut(s)	1089, 1940, 3131
GsuI	CTGGAG	2 cut(s)	154, 1769
HaeII	RGCGCY	1 cut(s)	1633
HapII	CCGG	5 cut(s)	29, 61, 1711, 3173, 3338
HgaI	GACGC	3 cut(s)	53, 845, 2314
HhaI	GCGC	4 cut(s)	192, 459, 1632, 2372
Hin1I	GRCGYC	3 cut(s)	64, 1630, 2306
Hin6I	GCGC	4 cut(s)	190, 457, 1630, 2370
HinP1I	GCGC	4 cut(s)	190, 457, 1630, 2370
HincII	GTYRAC	3 cut(s)	544, 1926, 2860
HindII	GTYRAC	3 cut(s)	544, 1926, 2860
HindIII	AAGCTT	1 cut(s)	2078
HpaII	CCGG	5 cut(s)	29, 61, 1711, 3173, 3338
Hpy166II	GTNNAC	8 cut(s)	544, 1533, 1571, 1849, 1926, 1963, 2860, 3291
Hpy8I	GTNNAC	8 cut(s)	544, 1533, 1571, 1849, 1926, 1963, 2860, 3291
Hpy99I	CGWCG	2 cut(s)	468, 2308
HpyCH4III	ACNGT	5 cut(s)	320, 1215, 2546, 3136, 3288
HpyCH4IV	ACGT	5 cut(s)	706, 1295, 2432, 2488, 3293
HpySE526I	ACGT	5 cut(s)	706, 1295, 2432, 2488, 3293
Hsp92I	GRCGYC	3 cut(s)	64, 1630, 2306
HspAI	GCGC	4 cut(s)	190, 457, 1630, 2370
KasI	GGCGCC	1 cut(s)	1629
KroI	GCCGGC	2 cut(s)	28, 60
KroNI	GCCGGC	2 cut(s)	30, 62
LguI	GCTCTTC	1 cut(s)	2706
MaeI	CTAG	8 cut(s)	983, 1761, 1968, 2219, 2247, 2390, 2958, 3141
MaeII	ACGT	5 cut(s)	706, 1295, 2432, 2488, 3293
MaeIII	GTNAC	5 cut(s)	1037, 3009, 3041, 3136, 3264
MbiI	CCGCTC	2 cut(s)	267, 3490
MfeI	CAATTG	2 cut(s)	1409, 1611
MflI	RGATCY	5 cut(s)	953, 1493, 2528, 3325, 3416
MhlI	GDGCHC	5 cut(s)	912, 923, 1965, 2585, 3154
MlsI	TGGCCA	2 cut(s)	1338, 3399
MluNI	TGGCCA	2 cut(s)	1338, 3399
Mly113I	GGCGCC	1 cut(s)	1630
MlyI	GAGTC	3 cut(s)	884, 2855, 3473
MmeI	TCCRAC	2 cut(s)	1750, 2556
Mox20I	TGGCCA	2 cut(s)	1338, 3399
Mph1103I	ATGCAT	3 cut(s)	6, 313, 993
MreI	CGCCGGCG	1 cut(s)	28
MroNI	GCCGGC	2 cut(s)	28, 60
MroXI	GAANNNNTTC	1 cut(s)	2397
MscI	TGGCCA	2 cut(s)	1338, 3399
MseI	TTAA	5 cut(s)	1298, 1742, 1790, 2669, 3596
MslI	CAYNNNNRTG	4 cut(s)	1044, 1950, 3405, 3431
Msp20I	TGGCCA	2 cut(s)	1338, 3399
MspA1I	CMGCKG	3 cut(s)	48, 1940, 3127
MspI	CCGG	5 cut(s)	29, 61, 1711, 3173, 3338
MspR9I	CCNGG	6 cut(s)	917, 1043, 1281, 1635, 1671, 2162
MunI	CAATTG	2 cut(s)	1409, 1611
Mva1269I	GAATGC	2 cut(s)	2888, 3009
MvaI	CCWGG	6 cut(s)	917, 1043, 1281, 1635, 1671, 2162
MvnI	CGCG	3 cut(s)	82, 192, 2370
NaeI	GCCGGC	2 cut(s)	30, 62
NarI	GGCGCC	1 cut(s)	1630
NgoMIV	GCCGGC	2 cut(s)	28, 60
NheI	GCTAGC	1 cut(s)	1760
NlaIV	GGNNCC	9 cut(s)	199, 807, 921, 1485, 1631, 2634, 3021, 3306, 3586
NmeAIII	GCCGAG	2 cut(s)	421, 2107
NmuCI	GTSAC	5 cut(s)	1037, 3009, 3041, 3136, 3264
NruI	TCGCGA	1 cut(s)	82
NsiI	ATGCAT	3 cut(s)	6, 313, 993
NspI	RCATGY	5 cut(s)	91, 360, 991, 2699, 2883
NspV	TTCGAA	1 cut(s)	718
OliI	CACNNNNGTG	2 cut(s)	1044, 1950
PaeI	GCATGC	1 cut(s)	2699
PaeR7I	CTCGAG	1 cut(s)	2195
PaqCI	CACCTGC	2 cut(s)	2455, 3247
PceI	AGGCCT	1 cut(s)	2125
PciI	ACATGT	2 cut(s)	356, 2879
PciSI	GCTCTTC	1 cut(s)	2706
PcsI	WCGNNNNNNNCGW	2 cut(s)	778, 1825
PctI	GAATGC	2 cut(s)	2888, 3009
PdiI	GCCGGC	2 cut(s)	30, 62
PdmI	GAANNNNTTC	1 cut(s)	2397
PfeI	GAWTC	7 cut(s)	121, 298, 373, 881, 1562, 1883, 2603
PflMI	CCANNNNNTGG	1 cut(s)	802
Ple19I	CGATCG	1 cut(s)	17
PleI	GAGTC	3 cut(s)	884, 2855, 3472
PluTI	GGCGCC	1 cut(s)	1633
PpsI	GAGTC	3 cut(s)	884, 2855, 3472
PscI	ACATGT	2 cut(s)	356, 2879
PsiI	TTATAA	1 cut(s)	2934
Psp124BI	GAGCTC	1 cut(s)	912
Psp1406I	AACGTT	2 cut(s)	1295, 2488
Psp6I	CCWGG	6 cut(s)	915, 1041, 1279, 1633, 1669, 2160
PspEI	GGTNACC	1 cut(s)	1037
PspFI	CCCAGC	3 cut(s)	1085, 1936, 3127
PspGI	CCWGG	6 cut(s)	915, 1041, 1279, 1633, 1669, 2160
PspN4I	GGNNCC	9 cut(s)	199, 807, 921, 1485, 1631, 2634, 3021, 3306, 3586
PspOMI	GGGCCC	1 cut(s)	919
PstI	CTGCAG	3 cut(s)	2004, 2853, 3244
PstNI	CAGNNNCTG	1 cut(s)	2612
PsuI	RGATCY	5 cut(s)	953, 1493, 2528, 3325, 3416
PvuI	CGATCG	1 cut(s)	17
PvuII	CAGCTG	2 cut(s)	1940, 3127
RruI	TCGCGA	1 cut(s)	82
RsaI	GTAC	7 cut(s)	109, 1217, 1285, 2277, 2997, 3123, 3285
RsaNI	GTAC	7 cut(s)	108, 1216, 1284, 2276, 2996, 3122, 3284
RseI	CAYNNNNRTG	4 cut(s)	1044, 1950, 3405, 3431
SacI	GAGCTC	1 cut(s)	912
SapI	GCTCTTC	1 cut(s)	2706
SaqAI	TTAA	5 cut(s)	1298, 1742, 1790, 2669, 3596
SchI	GAGTC	3 cut(s)	884, 2855, 3473
ScrFI	CCNGG	6 cut(s)	917, 1043, 1281, 1635, 1671, 2162
SduI	GDGCHC	5 cut(s)	912, 923, 1965, 2585, 3154
SfcI	CTRYAG	3 cut(s)	2000, 2849, 3240
SfoI	GGCGCC	1 cut(s)	1631
Sfr274I	CTCGAG	1 cut(s)	2195
SfuI	TTCGAA	1 cut(s)	718
SgrAI	CRCCGGYG	1 cut(s)	28
SinI	GGWCC	6 cut(s)	959, 1274, 2651, 3019, 3065, 3492
SlaI	CTCGAG	1 cut(s)	2195
SmiMI	CAYNNNNRTG	4 cut(s)	1044, 1950, 3405, 3431
SmlI	CTYRAG	3 cut(s)	2195, 3057, 3518
SmoI	CTYRAG	3 cut(s)	2195, 3057, 3518
SphI	GCATGC	1 cut(s)	2699
SseBI	AGGCCT	1 cut(s)	2125
SspDI	GGCGCC	1 cut(s)	1629
SspI	AATATT	2 cut(s)	2478, 3226
SspMI	CTAG	8 cut(s)	983, 1761, 1968, 2219, 2247, 2390, 2958, 3141
SstI	GAGCTC	1 cut(s)	912
StuI	AGGCCT	1 cut(s)	2125
StyD4I	CCNGG	6 cut(s)	915, 1041, 1279, 1633, 1669, 2160
StyI	CCWWGG	6 cut(s)	796, 932, 965, 1253, 1329, 2676
TaaI	ACNGT	5 cut(s)	320, 1215, 2546, 3136, 3288
TaiI	ACGT	5 cut(s)	709, 1298, 2435, 2491, 3296
TaqI	TCGA	8 cut(s)	301, 718, 781, 912, 2196, 2986, 3154, 3555
TatI	WGTACW	1 cut(s)	3121
TauI	GCSGC	7 cut(s)	35, 167, 267, 1630, 2329, 3236, 3478
TfiI	GAWTC	7 cut(s)	121, 298, 373, 881, 1562, 1883, 2603
Tru1I	TTAA	5 cut(s)	1298, 1742, 1790, 2669, 3596
Tru9I	TTAA	5 cut(s)	1298, 1742, 1790, 2669, 3596
TscAI	CASTG	5 cut(s)	1810, 2064, 2277, 2445, 3033
TseFI	GTSAC	5 cut(s)	1037, 3009, 3041, 3136, 3264
Tsp45I	GTSAC	5 cut(s)	1037, 3009, 3041, 3136, 3264
TspGWI	ACGGA	2 cut(s)	55, 1817
TspRI	CASTG	5 cut(s)	1810, 2064, 2277, 2445, 3033
Van91I	CCANNNNNTGG	1 cut(s)	802
VneI	GTGCAC	1 cut(s)	1961
VpaK11BI	GGWCC	6 cut(s)	959, 1274, 2651, 3019, 3065, 3492
XapI	RAATTY	9 cut(s)	1074, 1512, 1652, 1692, 1865, 2396, 2742, 3617, 3637
XceI	RCATGY	5 cut(s)	91, 360, 991, 2699, 2883
XhoI	CTCGAG	1 cut(s)	2195
XmiI	GTMKAC	2 cut(s)	1532, 1848
XmnI	GAANNNNTTC	1 cut(s)	2397
XspI	CTAG	8 cut(s)	983, 1761, 1968, 2219, 2247, 2390, 2958, 3141
Zsp2I	ATGCAT	3 cut(s)	6, 313, 993

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.