Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011072

Physical Location & Seq

Genomic Coordinates Forward (+)

3243115 .. 3247969

Gene Structure 4855 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1028700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 834 bp

ATGAGAGGGCTACGAAGATTGCTTGGACGAAGTCTTTCGTCTCACAGAAGATGCCAACCTCCTCGGCATCGCAATGGCACCCAGTTCATCTCTCGCCGAACCTATTTCCTATCTGCTTATTCAGCTCCCACGACGTCGTACCTGGCTCACAACAACTCCCCCTTTTCCGACGCATCTCCTTTCAATTCTCTGAAATGGGGCATTCTCGGCGGGCAGAAGAGAAGCATGTTTATCCAAACTCAATCCACACCTAACCCTGCATCTCTAATGTTTTATCCTGGGAAGCCCGTTATGGAAGTTGGCAGTGCAGACTTCCCGAATGCTCGTTCGGCCATGAATTCACCACTTGCCAAAGCCCTTTATGGAATTGATGGAGTCACTCGAGTGTTTTATGGATCAGATTTCGTTACTGTAACAAAATCTGAAGATACTTCTTGGGATCTATTGAAGCCTGAAATCTTTGCAGCAATCATGGATTTTTATTCTTCTGGGCAGCCACTCTTTCTAGACGAAAAAGCTGCATCAGCAATGGACACGGCTATTCAAGAAGATGATTCAGAAATCGTTGCAATGATAAAAGAATTGTTGGAGACTCGCATCCGACCAGCAGTGCAAGATGATGGTGGGGACATTGAGTATAGGGGATTTGATCCAGAAACTGGAACAGTGAAATTGAGAATGCAAGGAGCGTGTAGTGGCTGCCCAAGCTCATCAGTCACCCTGAAATCTGGAATTGAGAATATGCTCATGCACTATGTACCTGAGGTCAAAAGTGTAGAGGAAGAGCTAGATCCTGATGATGAAAATGCAGCTCTAGCAGGTCAGATGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0005198 GO:0005488 GO:0005507 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008144 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nfu_N NifU

Protein Analysis

278

Amino Acids

30.56

Weight (kDa)

5.25

Isoelectric Point (pI)

55.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nfu_N	PF08712	78 - 164	7.7e-31	Scaffold protein Nfu/NifU N terminal
NifU	PF01106	192 - 260	1.4e-29	NifU-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011072)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51390 AT3G20970 AT3G20970
fragaria_vesca	FvH4_1g23510 FvH4_1g23510
malus_domestica	MD04G1028700.v1.1
prunus_persica	Prupe.2G079800_v2.0.a1
pyrus_communis	pycom04g02500
rosa_chinensis	RchiOBHm_Chr2g0118911
rosa_laevigata	RLG00000018435
rosa_multiflora	Rmu_sc0001653.1_g000031 Rmu_sc0003492.1_g000006
rosa_roxburghii	Rroxscaffold_2G00124590 Rroxscaffold_2G00144600
rosa_rugosa	Rorug02G0219500
rosa_samantha	Rh2AG276800 Rh2BG288500 Rh2DG283800 Rh2DG302900
rosa_wichuraiana	Rw2G022050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	137
Acc36I	ACCTGC	1 cut(s)	809
AccB1I	GGYRCC	1 cut(s)	77
AccB7I	CCANNNNNTGG	1 cut(s)	659
AciI	CCGC	1 cut(s)	210
AclWI	GGATC	4 cut(s)	403, 447, 644, 785
AcoI	YGGCCR	1 cut(s)	330
AcsI	RAATTY	1 cut(s)	337
AcuI	CTGAAG	1 cut(s)	444
AcyI	GRCGYC	1 cut(s)	134
AfaI	GTAC	2 cut(s)	140, 759
AfiI	CCNNNNNNNGG	1 cut(s)	659
AgsI	TTSAA	3 cut(s)	184, 448, 545
AjnI	CCWGG	2 cut(s)	141, 277
AleI	CACNNNNGTG	1 cut(s)	383
AluBI	AGCT	5 cut(s)	125, 518, 708, 787, 812
AluI	AGCT	5 cut(s)	125, 518, 708, 787, 812
Alw26I	GTCTC	2 cut(s)	45, 584
AlwI	GGATC	4 cut(s)	403, 447, 644, 785
AlwNI	CAGNNNCTG	1 cut(s)	659
Ama87I	CYCGRG	1 cut(s)	381
AoxI	GGCC	1 cut(s)	330
ApeKI	GCWGC	5 cut(s)	464, 493, 518, 699, 809
ApoI	RAATTY	1 cut(s)	337
Asp700I	GAANNNNTTC	1 cut(s)	34
AsuHPI	GGTGA	2 cut(s)	333, 709
AvaI	CYCGRG	1 cut(s)	381
AxyI	CCTNAGG	1 cut(s)	762
BanI	GGYRCC	1 cut(s)	77
BbvI	GCAGC	5 cut(s)	476, 505, 505, 686, 821
BccI	CCATC	3 cut(s)	365, 614, 820
BceAI	ACGGC	1 cut(s)	552
BcgI	CGANNNNNNTGC	2 cut(s)	500, 534
BciT130I	CCWGG	2 cut(s)	143, 279
BcoDI	GTCTC	2 cut(s)	45, 584
BfaI	CTAG	3 cut(s)	506, 788, 815
BfuAI	ACCTGC	1 cut(s)	809
BisI	GCNGC	5 cut(s)	465, 494, 519, 700, 810
BlsI	GCNGC	5 cut(s)	466, 495, 520, 701, 811
Bme1390I	CCNGG	2 cut(s)	143, 279
BmeT110I	CYCGRG	1 cut(s)	381
BmiI	GGNNCC	1 cut(s)	79
BmrFI	CCNGG	2 cut(s)	143, 279
BmrI	ACTGGG	1 cut(s)	76
BmsI	GCATC	6 cut(s)	41, 76, 182, 269, 530, 606
BmuI	ACTGGG	1 cut(s)	76
BsaHI	GRCGYC	1 cut(s)	134
BsaJI	CCNNGG	2 cut(s)	62, 278
BsaXI	ACNNNNNCTCC	2 cut(s)	140, 170
Bsc4I	CCNNNNNNNGG	1 cut(s)	659
Bse1I	ACTGG	2 cut(s)	82, 664
Bse21I	CCTNAGG	1 cut(s)	762
Bse3DI	GCAATG	3 cut(s)	79, 534, 576
BseBI	CCWGG	2 cut(s)	143, 279
BseDI	CCNNGG	2 cut(s)	62, 278
BseGI	GGATG	1 cut(s)	597
BseLI	CCNNNNNNNGG	1 cut(s)	659
BseMI	GCAATG	3 cut(s)	79, 534, 576
BseMII	CTCAG	1 cut(s)	753
BseNI	ACTGG	2 cut(s)	82, 664
BseRI	GAGGAG	1 cut(s)	51
BseXI	GCAGC	5 cut(s)	476, 505, 505, 686, 821
BsgI	GTGCAG	1 cut(s)	327
BshFI	GGCC	1 cut(s)	332
BshNI	GGYRCC	1 cut(s)	77
BsiHKCI	CYCGRG	1 cut(s)	381
BslFI	GGGAC	1 cut(s)	641
BslI	CCNNNNNNNGG	1 cut(s)	659
BsmAI	GTCTC	2 cut(s)	45, 584
BsmBI	CGTCTC	1 cut(s)	45
BsmFI	GGGAC	1 cut(s)	641
BsmI	GAATGC	3 cut(s)	201, 325, 684
BsnI	GGCC	1 cut(s)	332
BsoBI	CYCGRG	1 cut(s)	381
Bsp143I	GATC	4 cut(s)	395, 439, 649, 790
BspACI	CCGC	1 cut(s)	210
BspANI	GGCC	1 cut(s)	332
BspCNI	CTCAG	1 cut(s)	754
BspLI	GGNNCC	1 cut(s)	79
BspMI	ACCTGC	1 cut(s)	809
BspPI	GGATC	4 cut(s)	403, 447, 644, 785
BspQI	GCTCTTC	1 cut(s)	777
BspT107I	GGYRCC	1 cut(s)	77
BsrDI	GCAATG	3 cut(s)	79, 534, 576
BsrI	ACTGG	2 cut(s)	82, 664
BssECI	CCNNGG	2 cut(s)	62, 278
BssMI	GATC	4 cut(s)	395, 439, 649, 790
BssNI	GRCGYC	1 cut(s)	134
Bst2UI	CCWGG	2 cut(s)	143, 279
Bst4CI	ACNGT	2 cut(s)	412, 667
Bst6I	CTCTTC	2 cut(s)	212, 777
BstACI	GRCGYC	1 cut(s)	134
BstC8I	GCNNGC	1 cut(s)	212
BstDEI	CTNAG	1 cut(s)	762
BstF5I	GGATG	1 cut(s)	597
BstKTI	GATC	4 cut(s)	398, 442, 652, 793
BstMAI	GTCTC	2 cut(s)	45, 584
BstMBI	GATC	4 cut(s)	395, 439, 649, 790
BstMWI	GCNNNNNNNGC	6 cut(s)	122, 207, 329, 524, 705, 815
BstNI	CCWGG	2 cut(s)	143, 279
BstNSI	RCATGY	1 cut(s)	229
BstSCI	CCNGG	2 cut(s)	141, 277
BstV1I	GCAGC	5 cut(s)	476, 505, 505, 686, 821
BstX2I	RGATCY	2 cut(s)	439, 790
BstYI	RGATCY	2 cut(s)	439, 790
Bsu36I	CCTNAGG	1 cut(s)	762
BsuRI	GGCC	1 cut(s)	332
BtgZI	GCGATG	1 cut(s)	53
BtsCI	GGATG	1 cut(s)	597
BtsI	GCAGTG	2 cut(s)	310, 615
BtsIMutI	CAGTG	3 cut(s)	310, 615, 672
BveI	ACCTGC	1 cut(s)	809
Cac8I	GCNNGC	1 cut(s)	212
CaiI	CAGNNNCTG	1 cut(s)	659
CseI	GACGC	1 cut(s)	179
Csp6I	GTAC	2 cut(s)	139, 758
CviAII	CATG	4 cut(s)	226, 334, 472, 748
CviQI	GTAC	2 cut(s)	139, 758
DdeI	CTNAG	1 cut(s)	762
DpnI	GATC	4 cut(s)	397, 441, 651, 792
DpnII	GATC	4 cut(s)	395, 439, 649, 790
EaeI	YGGCCR	1 cut(s)	330
Eam1104I	CTCTTC	2 cut(s)	212, 777
EarI	CTCTTC	2 cut(s)	212, 777
Eco57I	CTGAAG	1 cut(s)	444
Eco81I	CCTNAGG	1 cut(s)	762
Eco88I	CYCGRG	1 cut(s)	381
EcoRI	GAATTC	1 cut(s)	337
EcoRII	CCWGG	2 cut(s)	141, 277
Esp3I	CGTCTC	1 cut(s)	45
FaeI	CATG	4 cut(s)	229, 337, 475, 751
FaqI	GGGAC	1 cut(s)	641
FatI	CATG	4 cut(s)	225, 333, 471, 747
FauI	CCCGC	1 cut(s)	203
Fnu4HI	GCNGC	5 cut(s)	465, 494, 519, 700, 810
FokI	GGATG	1 cut(s)	584
Fsp4HI	GCNGC	5 cut(s)	465, 494, 519, 700, 810
FspBI	CTAG	3 cut(s)	506, 788, 815
GluI	GCNGC	5 cut(s)	465, 494, 519, 700, 810
HaeIII	GGCC	1 cut(s)	332
HgaI	GACGC	1 cut(s)	179
Hin1I	GRCGYC	1 cut(s)	134
Hin1II	CATG	4 cut(s)	229, 337, 475, 751
HinfI	GANTC	3 cut(s)	375, 554, 592
HphI	GGTGA	2 cut(s)	333, 709
Hpy188I	TCNGA	7 cut(s)	169, 192, 400, 424, 559, 602, 825
Hpy188III	TCNNGA	6 cut(s)	316, 506, 545, 653, 729, 794
Hpy99I	CGWCG	3 cut(s)	136, 139, 173
HpyCH4III	ACNGT	2 cut(s)	412, 667
HpyCH4IV	ACGT	1 cut(s)	134
HpyCH4V	TGCA	9 cut(s)	260, 308, 464, 521, 569, 613, 682, 751, 809
HpyF10VI	GCNNNNNNNGC	6 cut(s)	122, 207, 329, 524, 705, 815
HpyF3I	CTNAG	1 cut(s)	762
HpySE526I	ACGT	1 cut(s)	134
Hsp92I	GRCGYC	1 cut(s)	134
Hsp92II	CATG	4 cut(s)	229, 337, 475, 751
Kzo9I	GATC	4 cut(s)	395, 439, 649, 790
LguI	GCTCTTC	1 cut(s)	777
LmnI	GCTCC	2 cut(s)	130, 686
Lsp1109I	GCAGC	5 cut(s)	476, 505, 505, 686, 821
LweI	GCATC	6 cut(s)	41, 76, 182, 269, 530, 606
MaeI	CTAG	3 cut(s)	506, 788, 815
MaeII	ACGT	1 cut(s)	134
MaeIII	GTNAC	4 cut(s)	376, 406, 412, 715
MalI	GATC	4 cut(s)	397, 441, 651, 792
MboI	GATC	4 cut(s)	395, 439, 649, 790
MboII	GAAGA	7 cut(s)	27, 60, 229, 437, 477, 560, 794
MflI	RGATCY	2 cut(s)	439, 790
MluCI	AATT	6 cut(s)	184, 337, 366, 581, 671, 732
MlyI	GAGTC	2 cut(s)	384, 586
MmeI	TCCRAC	3 cut(s)	192, 567, 625
MnlI	CCTC	4 cut(s)	69, 72, 757, 772
MroXI	GAANNNNTTC	1 cut(s)	34
MslI	CAYNNNNRTG	2 cut(s)	72, 383
MspR9I	CCNGG	2 cut(s)	143, 279
Mva1269I	GAATGC	3 cut(s)	201, 325, 684
MvaI	CCWGG	2 cut(s)	143, 279
MwoI	GCNNNNNNNGC	6 cut(s)	122, 207, 329, 524, 705, 815
NdeII	GATC	4 cut(s)	395, 439, 649, 790
NlaIII	CATG	4 cut(s)	229, 337, 475, 751
NlaIV	GGNNCC	1 cut(s)	79
NmeAIII	GCCGAG	2 cut(s)	43, 186
NmuCI	GTSAC	2 cut(s)	376, 715
NspI	RCATGY	1 cut(s)	229
OliI	CACNNNNGTG	1 cut(s)	383
PaeR7I	CTCGAG	1 cut(s)	381
PciSI	GCTCTTC	1 cut(s)	777
PctI	GAATGC	3 cut(s)	201, 325, 684
PdmI	GAANNNNTTC	1 cut(s)	34
PfeI	GAWTC	1 cut(s)	554
PflFI	GACNNNGTC	1 cut(s)	30
PflMI	CCANNNNNTGG	1 cut(s)	659
PkrI	GCNGC	5 cut(s)	466, 495, 520, 701, 811
PleI	GAGTC	2 cut(s)	383, 586
PpsI	GAGTC	2 cut(s)	383, 586
Psp6I	CCWGG	2 cut(s)	141, 277
PspGI	CCWGG	2 cut(s)	141, 277
PspN4I	GGNNCC	1 cut(s)	79
PspXI	VCTCGAGB	1 cut(s)	381
PstNI	CAGNNNCTG	1 cut(s)	659
PsuI	RGATCY	2 cut(s)	439, 790
PsyI	GACNNNGTC	1 cut(s)	30
RsaI	GTAC	2 cut(s)	140, 759
RsaNI	GTAC	2 cut(s)	139, 758
RseI	CAYNNNNRTG	2 cut(s)	72, 383
SapI	GCTCTTC	1 cut(s)	777
SatI	GCNGC	5 cut(s)	465, 494, 519, 700, 810
Sau3AI	GATC	4 cut(s)	395, 439, 649, 790
SchI	GAGTC	2 cut(s)	384, 586
ScrFI	CCNGG	2 cut(s)	143, 279
SfaNI	GCATC	6 cut(s)	41, 76, 182, 269, 530, 606
Sfr274I	CTCGAG	1 cut(s)	381
SlaI	CTCGAG	1 cut(s)	381
SmiMI	CAYNNNNRTG	2 cut(s)	72, 383
SmlI	CTYRAG	1 cut(s)	381
SmoI	CTYRAG	1 cut(s)	381
Sse9I	AATT	6 cut(s)	184, 337, 366, 581, 671, 732
SsiI	CCGC	1 cut(s)	210
SspMI	CTAG	3 cut(s)	506, 788, 815
StyD4I	CCNGG	2 cut(s)	141, 277
TaaI	ACNGT	2 cut(s)	412, 667
TaiI	ACGT	1 cut(s)	137
TaqI	TCGA	1 cut(s)	382
TasI	AATT	6 cut(s)	184, 337, 366, 581, 671, 732
TfiI	GAWTC	1 cut(s)	554
TscAI	CASTG	3 cut(s)	310, 615, 672
TseFI	GTSAC	2 cut(s)	376, 715
TseI	GCWGC	5 cut(s)	464, 493, 518, 699, 809
Tsp45I	GTSAC	2 cut(s)	376, 715
TspDTI	ATGAA	3 cut(s)	76, 350, 816
TspRI	CASTG	3 cut(s)	310, 615, 672
Tth111I	GACNNNGTC	1 cut(s)	30
Van91I	CCANNNNNTGG	1 cut(s)	659
XapI	RAATTY	1 cut(s)	337
XbaI	TCTAGA	1 cut(s)	505
XceI	RCATGY	1 cut(s)	229
XhoI	CTCGAG	1 cut(s)	381
XmnI	GAANNNNTTC	1 cut(s)	34
XspI	CTAG	3 cut(s)	506, 788, 815
ZraI	GACGTC	1 cut(s)	135

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.