Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0007048

Physical Location & Seq

Genomic Coordinates Reverse (-)

3758491 .. 3763450

Gene Structure 4960 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1033300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGAAAGCTATTTTCACAGCACCACACACCACCTCACTATTCCGCATAGCCACCAACCGCTTCAAAATCCCAGTCCCTTCCTTCATCCGAAGCACCATGTCTGATTCCGATCACCAATCCCTCCCTCCTTTTTCCTCCCCAAGAATGGTCGTCAAGAAGGTCCTCGCCAAGACCCAGCACGAAGGCGATGGTGCTGTTGCCAGGAGAGCCATTGGAAGCCGCGAGTTGAGGAACCTGGATCCTTTTCTGATGCTGGATCACTTTTCAGTGTCCCCTCCTGCTGGATTTCCTGACCACCCACACAGAGGTTTCGAGACTGTCACATACATGTTACAGGGAGCAATCACTCACCAAGACTTTGCAGGGCACAGGGGTACAATCCGGACTGGGGATGTGCAGTGGATGACGGCAGGCAGGGGAATAGTTCATTCAGAAATGCCTGAAGGAGAAGGACCCAACACTGGTTTGCAGCTTTGGATCAATTTAGCCCAGAAGGACAAAATGATTGAGCCAAGATACCAAGAACTCCTAAGCAAGGACATAACTAGGGCAGAGAAAGACGGGGTCGACGTCCGAGTTGTCGCCGGAGAAGCATTGGGAGTCAGCTCCCCAGTTTACACCAGAACACCAGCAATGTTTCTAGATTTCGTCTTAAAACCGAGAGCTCAACTTCATCACTGCATACCGGAAGCCTGGACTTCATTTGTGTACGTAATCGAAGGAGAAGGGGTGTTTGGATCAACTCATTCGTCAGCTCATCACGTCTTGGTTTTGGGTCCGGGAGACGGCCTAAGCGTGTGCAACAAGTCATCGGAGCCGTTGCGGTTTTTGTTGGCGGCAGGGCAACCCTTAAATGAACCAGTGGTTTCGCATGGCCCTTTTGTGATGAACACACAGGAGGAAATCTACCAAACTATTCAGGACTATCACCATGGCATGAATGGGTTTGAAATGGCCAAGACTTGGAGATCTCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004857 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009637 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009785 GO:0009892 GO:0009987 GO:0010033 GO:0010466 GO:0010605 GO:0016020 GO:0019222 GO:0023052 GO:0030162 GO:0030234 GO:0030414 GO:0030522 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0032870 GO:0033993 GO:0042221 GO:0042742 GO:0043086 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0051704 GO:0051707 GO:0051716 GO:0052547 GO:0060255 GO:0061134 GO:0065007 GO:0065009 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071478 GO:0071482 GO:0071483 GO:0071495 GO:0071944 GO:0080090 GO:0097305 GO:0097306 GO:0098542 GO:0098772 GO:0104004 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pirin Pirin_C

Protein Analysis

326

Amino Acids

36.06

Weight (kDa)

6.79

Isoelectric Point (pI)

42.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pirin	PF02678	67 - 160	2.7e-33	Pirin
Pirin_C	PF05726	214 - 314	4.8e-30	Pirin C-terminal cupin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007048)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43120 AT2G43120 AT3G59220 AT3G59260
fragaria_vesca	FvH4_4g11770 FvH4_4g11770 FvH4_4g11770 FvH4_4g11770
malus_domestica	MD04G1033300.v1.1
prunus_persica	Prupe.1G181100_v2.0.a1
pyrus_communis	pycom04g02740 pycom04g02780
rosa_chinensis	RchiOBHm_Chr4g0409021
rosa_laevigata	RLG00000008595
rosa_multiflora	Rmu_sc0000121.1_g000003 Rmu_sc0006766.1_g000003 Rmu_sc0017774.1_g000001 Rmu_sc0022473.1_g000002 Rmu_sc0026065.1_g000003
rosa_roxburghii	Rroxscaffold_5G00353500
rosa_rugosa	Rorug04G0089800
rosa_samantha	Rh4AG153500 Rh4BG151600 Rh4CG162000 Rh4DG146900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	573
AccB7I	CCANNNNNTGG	1 cut(s)	963
AccI	GTMKAC	1 cut(s)	567
AccII	CGCG	1 cut(s)	222
AccIII	TCCGGA	1 cut(s)	381
AciI	CCGC	5 cut(s)	43, 58, 220, 823, 836
AclWI	GGATC	5 cut(s)	233, 246, 264, 485, 745
AcoI	YGGCCR	1 cut(s)	954
AcuI	CTGAAG	1 cut(s)	462
AcyI	GRCGYC	1 cut(s)	570
AfaI	GTAC	2 cut(s)	376, 710
AfiI	CCNNNNNNNGG	8 cut(s)	145, 281, 305, 388, 461, 535, 785, 963
AflIII	ACRYGT	1 cut(s)	327
AgsI	TTSAA	2 cut(s)	64, 950
AjiI	CACGTC	1 cut(s)	763
AjnI	CCWGG	3 cut(s)	200, 234, 692
AjuI	GAANNNNNNNTTGG	2 cut(s)	717, 749
AluBI	AGCT	5 cut(s)	8, 472, 606, 665, 755
AluI	AGCT	5 cut(s)	8, 472, 606, 665, 755
Alw21I	GWGCWC	1 cut(s)	667
Alw26I	GTCTC	2 cut(s)	308, 777
AlwI	GGATC	5 cut(s)	233, 246, 264, 485, 745
Aor13HI	TCCGGA	1 cut(s)	381
AoxI	GGCC	3 cut(s)	787, 874, 954
ApeKI	GCWGC	1 cut(s)	469
ArsI	GACNNNNNNTTYG	2 cut(s)	57, 89
AspS9I	GGNCC	4 cut(s)	160, 452, 776, 875
AsuC2I	CCSGG	1 cut(s)	780
AsuHPI	GGTGA	3 cut(s)	104, 341, 920
AvaII	GGWCC	3 cut(s)	160, 452, 776
BaeGI	GKGCMC	1 cut(s)	369
BalI	TGGCCA	1 cut(s)	956
BamHI	GGATCC	1 cut(s)	238
BanII	GRGCYC	1 cut(s)	667
Bbv12I	GWGCWC	1 cut(s)	667
BbvI	GCAGC	1 cut(s)	481
BccI	CCATC	1 cut(s)	182
BceAI	ACGGC	3 cut(s)	423, 802, 802
BciT130I	CCWGG	3 cut(s)	202, 236, 694
BcnI	CCSGG	1 cut(s)	780
BcoDI	GTCTC	2 cut(s)	308, 777
BfaI	CTAG	2 cut(s)	546, 641
BglII	AGATCT	1 cut(s)	968
BisI	GCNGC	3 cut(s)	220, 470, 837
BlsI	GCNGC	3 cut(s)	221, 471, 838
Bme1390I	CCNGG	4 cut(s)	202, 236, 694, 780
Bme18I	GGWCC	3 cut(s)	160, 452, 776
BmgBI	CACGTC	1 cut(s)	763
BmgT120I	GGNCC	4 cut(s)	160, 452, 776, 875
BmiI	GGNNCC	5 cut(s)	233, 240, 454, 777, 816
BmrFI	CCNGG	4 cut(s)	202, 236, 694, 780
BmrI	ACTGGG	3 cut(s)	65, 396, 605
BmsI	GCATC	1 cut(s)	240
BmuI	ACTGGG	3 cut(s)	65, 396, 605
Bpu10I	CCTNAGC	2 cut(s)	530, 791
BpuMI	CCSGG	1 cut(s)	780
BsaAI	YACGTR	1 cut(s)	712
BsaBI	GATNNNNATC	1 cut(s)	108
BsaHI	GRCGYC	1 cut(s)	570
BsaJI	CCNNGG	1 cut(s)	931
BsaWI	WCCGGW	2 cut(s)	381, 685
BsaXI	ACNNNNNCTCC	2 cut(s)	714, 744
Bsc4I	CCNNNNNNNGG	8 cut(s)	145, 281, 305, 388, 461, 535, 785, 963
Bse1I	ACTGG	5 cut(s)	71, 391, 466, 611, 860
Bse3DI	GCAATG	1 cut(s)	639
Bse8I	GATNNNNATC	1 cut(s)	108
BseAI	TCCGGA	1 cut(s)	381
BseBI	CCWGG	3 cut(s)	202, 236, 694
BseDI	CCNNGG	1 cut(s)	931
BseGI	GGATG	3 cut(s)	84, 397, 408
BseJI	GATNNNNATC	1 cut(s)	108
BseLI	CCNNNNNNNGG	8 cut(s)	145, 281, 305, 388, 461, 535, 785, 963
BseMI	GCAATG	1 cut(s)	639
BseNI	ACTGG	5 cut(s)	71, 391, 466, 611, 860
BseSI	GKGCMC	1 cut(s)	369
BseXI	GCAGC	1 cut(s)	481
BseYI	CCCAGC	1 cut(s)	174
BsgI	GTGCAG	1 cut(s)	416
Bsh1236I	CGCG	1 cut(s)	222
BshFI	GGCC	3 cut(s)	789, 876, 956
BsiHKAI	GWGCWC	1 cut(s)	667
BsiSI	CCGG	4 cut(s)	382, 585, 686, 779
BslFI	GGGAC	2 cut(s)	59, 256
BslI	CCNNNNNNNGG	8 cut(s)	145, 281, 305, 388, 461, 535, 785, 963
BsmAI	GTCTC	2 cut(s)	308, 777
BsmBI	CGTCTC	1 cut(s)	777
BsmFI	GGGAC	2 cut(s)	59, 256
BsnI	GGCC	3 cut(s)	789, 876, 956
Bsp1286I	GDGCHC	2 cut(s)	369, 667
Bsp13I	TCCGGA	1 cut(s)	381
Bsp143I	GATC	6 cut(s)	109, 238, 256, 477, 737, 968
Bsp19I	CCATGG	1 cut(s)	931
BspACI	CCGC	5 cut(s)	43, 58, 220, 823, 836
BspANI	GGCC	3 cut(s)	789, 876, 956
BspEI	TCCGGA	1 cut(s)	381
BspFNI	CGCG	1 cut(s)	222
BspLI	GGNNCC	5 cut(s)	233, 240, 454, 777, 816
BspPI	GGATC	5 cut(s)	233, 246, 264, 485, 745
BsrDI	GCAATG	1 cut(s)	639
BsrI	ACTGG	5 cut(s)	71, 391, 466, 611, 860
BssECI	CCNNGG	1 cut(s)	931
BssMI	GATC	6 cut(s)	109, 238, 256, 477, 737, 968
BssNI	GRCGYC	1 cut(s)	570
BssT1I	CCWWGG	1 cut(s)	931
Bst2UI	CCWGG	3 cut(s)	202, 236, 694
Bst4CI	ACNGT	1 cut(s)	319
BstACI	GRCGYC	1 cut(s)	570
BstBAI	YACGTR	1 cut(s)	712
BstC8I	GCNNGC	1 cut(s)	412
BstDEI	CTNAG	2 cut(s)	530, 791
BstDSI	CCRYGG	1 cut(s)	931
BstENI	CCTNNNNNAGG	1 cut(s)	533
BstF5I	GGATG	3 cut(s)	84, 397, 408
BstFNI	CGCG	1 cut(s)	222
BstKTI	GATC	6 cut(s)	112, 241, 259, 480, 740, 971
BstMAI	GTCTC	2 cut(s)	308, 777
BstMBI	GATC	6 cut(s)	109, 238, 256, 477, 737, 968
BstMWI	GCNNNNNNNGC	2 cut(s)	206, 590
BstNI	CCWGG	3 cut(s)	202, 236, 694
BstNSI	RCATGY	1 cut(s)	331
BstSCI	CCNGG	4 cut(s)	200, 234, 692, 778
BstSLI	GKGCMC	1 cut(s)	369
BstSNI	TACGTA	1 cut(s)	712
BstUI	CGCG	1 cut(s)	222
BstV1I	GCAGC	1 cut(s)	481
BstX2I	RGATCY	2 cut(s)	238, 968
BstYI	RGATCY	2 cut(s)	238, 968
BsuRI	GGCC	3 cut(s)	789, 876, 956
BtgI	CCRYGG	1 cut(s)	931
BtgZI	GCGATG	1 cut(s)	201
BtrI	CACGTC	1 cut(s)	763
BtsCI	GGATG	3 cut(s)	84, 397, 408
BtsI	GCAGTG	2 cut(s)	404, 676
BtsIMutI	CAGTG	5 cut(s)	273, 404, 459, 676, 867
Cac8I	GCNNGC	1 cut(s)	412
Cfr13I	GGNCC	4 cut(s)	160, 452, 776, 875
Csp6I	GTAC	2 cut(s)	375, 709
CviAII	CATG	5 cut(s)	97, 328, 872, 932, 937
CviQI	GTAC	2 cut(s)	375, 709
DdeI	CTNAG	2 cut(s)	530, 791
DpnI	GATC	6 cut(s)	111, 240, 258, 479, 739, 970
DpnII	GATC	6 cut(s)	109, 238, 256, 477, 737, 968
EaeI	YGGCCR	1 cut(s)	954
Ecl136II	GAGCTC	1 cut(s)	665
Eco105I	TACGTA	1 cut(s)	712
Eco130I	CCWWGG	1 cut(s)	931
Eco24I	GRGCYC	1 cut(s)	667
Eco47I	GGWCC	3 cut(s)	160, 452, 776
Eco53kI	GAGCTC	1 cut(s)	665
Eco57I	CTGAAG	1 cut(s)	462
EcoICRI	GAGCTC	1 cut(s)	665
EcoNI	CCTNNNNNAGG	1 cut(s)	533
EcoO109I	RGGNCCY	2 cut(s)	160, 452
EcoRII	CCWGG	3 cut(s)	200, 234, 692
EcoT14I	CCWWGG	1 cut(s)	931
EcoT38I	GRGCYC	1 cut(s)	667
ErhI	CCWWGG	1 cut(s)	931
Esp3I	CGTCTC	1 cut(s)	777
FaeI	CATG	5 cut(s)	100, 331, 875, 935, 940
FaiI	YATR	9 cut(s)	47, 98, 325, 329, 542, 683, 873, 933, 938
FaqI	GGGAC	2 cut(s)	59, 256
FatI	CATG	5 cut(s)	96, 327, 871, 931, 936
FblI	GTMKAC	1 cut(s)	567
Fnu4HI	GCNGC	3 cut(s)	220, 470, 837
FokI	GGATG	3 cut(s)	71, 404, 415
FriOI	GRGCYC	1 cut(s)	667
Fsp4HI	GCNGC	3 cut(s)	220, 470, 837
FspBI	CTAG	2 cut(s)	546, 641
GluI	GCNGC	3 cut(s)	220, 470, 837
GsaI	CCCAGC	1 cut(s)	178
HaeIII	GGCC	3 cut(s)	789, 876, 956
HapII	CCGG	4 cut(s)	382, 585, 686, 779
Hin1I	GRCGYC	1 cut(s)	570
Hin1II	CATG	5 cut(s)	100, 331, 875, 935, 940
HincII	GTYRAC	1 cut(s)	568
HindII	GTYRAC	1 cut(s)	568
HinfI	GANTC	2 cut(s)	104, 600
HpaII	CCGG	4 cut(s)	382, 585, 686, 779
HphI	GGTGA	3 cut(s)	104, 341, 920
Hpy166II	GTNNAC	3 cut(s)	568, 616, 709
Hpy188I	TCNGA	7 cut(s)	89, 103, 109, 249, 433, 575, 814
Hpy188III	TCNNGA	6 cut(s)	154, 290, 313, 382, 641, 920
Hpy8I	GTNNAC	3 cut(s)	568, 616, 709
Hpy99I	CGWCG	1 cut(s)	572
HpyAV	CCTTC	9 cut(s)	87, 91, 151, 176, 437, 443, 487, 713, 719
HpyCH4III	ACNGT	1 cut(s)	319
HpyCH4IV	ACGT	3 cut(s)	570, 711, 762
HpyCH4V	TGCA	5 cut(s)	362, 397, 469, 681, 801
HpyF10VI	GCNNNNNNNGC	2 cut(s)	206, 590
HpyF3I	CTNAG	2 cut(s)	530, 791
HpySE526I	ACGT	3 cut(s)	570, 711, 762
Hsp92I	GRCGYC	1 cut(s)	570
Hsp92II	CATG	5 cut(s)	100, 331, 875, 935, 940
Kpn2I	TCCGGA	1 cut(s)	381
Kzo9I	GATC	6 cut(s)	109, 238, 256, 477, 737, 968
LmnI	GCTCC	3 cut(s)	338, 611, 814
Lsp1109I	GCAGC	1 cut(s)	481
LweI	GCATC	1 cut(s)	240
MaeI	CTAG	2 cut(s)	546, 641
MaeII	ACGT	3 cut(s)	570, 711, 762
MaeIII	GTNAC	2 cut(s)	319, 330
MalI	GATC	6 cut(s)	111, 240, 258, 479, 739, 970
MboI	GATC	6 cut(s)	109, 238, 256, 477, 737, 968
MflI	RGATCY	2 cut(s)	238, 968
MhlI	GDGCHC	2 cut(s)	369, 667
MlsI	TGGCCA	1 cut(s)	956
MluCI	AATT	1 cut(s)	481
MluNI	TGGCCA	1 cut(s)	956
MlyI	GAGTC	1 cut(s)	609
MnlI	CCTC	9 cut(s)	43, 131, 135, 145, 173, 222, 285, 299, 892
Mox20I	TGGCCA	1 cut(s)	956
MroI	TCCGGA	1 cut(s)	381
MscI	TGGCCA	1 cut(s)	956
MseI	TTAA	2 cut(s)	653, 851
MslI	CAYNNNNRTG	1 cut(s)	326
Msp20I	TGGCCA	1 cut(s)	956
MspI	CCGG	4 cut(s)	382, 585, 686, 779
MspR9I	CCNGG	4 cut(s)	202, 236, 694, 780
MvaI	CCWGG	3 cut(s)	202, 236, 694
MvnI	CGCG	1 cut(s)	222
MwoI	GCNNNNNNNGC	2 cut(s)	206, 590
NciI	CCSGG	1 cut(s)	780
NcoI	CCATGG	1 cut(s)	931
NdeII	GATC	6 cut(s)	109, 238, 256, 477, 737, 968
NlaIII	CATG	5 cut(s)	100, 331, 875, 935, 940
NlaIV	GGNNCC	5 cut(s)	233, 240, 454, 777, 816
NmuCI	GTSAC	1 cut(s)	319
NspI	RCATGY	1 cut(s)	331
PciI	ACATGT	1 cut(s)	327
PcsI	WCGNNNNNNNCGW	2 cut(s)	567, 792
PfeI	GAWTC	1 cut(s)	104
PflFI	GACNNNGTC	1 cut(s)	563
PflMI	CCANNNNNTGG	1 cut(s)	963
PfoI	TCCNGGA	1 cut(s)	778
PkrI	GCNGC	3 cut(s)	221, 471, 838
PleI	GAGTC	1 cut(s)	608
PpsI	GAGTC	1 cut(s)	608
Ppu21I	YACGTR	1 cut(s)	712
PpuMI	RGGWCCY	2 cut(s)	160, 452
PscI	ACATGT	1 cut(s)	327
Psp124BI	GAGCTC	1 cut(s)	667
Psp5II	RGGWCCY	2 cut(s)	160, 452
Psp6I	CCWGG	3 cut(s)	200, 234, 692
PspFI	CCCAGC	1 cut(s)	174
PspGI	CCWGG	3 cut(s)	200, 234, 692
PspN4I	GGNNCC	5 cut(s)	233, 240, 454, 777, 816
PspPI	GGNCC	4 cut(s)	160, 452, 776, 875
PspPPI	RGGWCCY	2 cut(s)	160, 452
PsuI	RGATCY	2 cut(s)	238, 968
PsyI	GACNNNGTC	1 cut(s)	563
RsaI	GTAC	2 cut(s)	376, 710
RsaNI	GTAC	2 cut(s)	375, 709
RseI	CAYNNNNRTG	1 cut(s)	326
SacI	GAGCTC	1 cut(s)	667
SalI	GTCGAC	1 cut(s)	566
SaqAI	TTAA	2 cut(s)	653, 851
SatI	GCNGC	3 cut(s)	220, 470, 837
Sau3AI	GATC	6 cut(s)	109, 238, 256, 477, 737, 968
Sau96I	GGNCC	4 cut(s)	160, 452, 776, 875
SchI	GAGTC	1 cut(s)	609
ScrFI	CCNGG	4 cut(s)	202, 236, 694, 780
SduI	GDGCHC	2 cut(s)	369, 667
SfaNI	GCATC	1 cut(s)	240
SinI	GGWCC	3 cut(s)	160, 452, 776
SmiMI	CAYNNNNRTG	1 cut(s)	326
SnaBI	TACGTA	1 cut(s)	712
Sse9I	AATT	1 cut(s)	481
SsiI	CCGC	5 cut(s)	43, 58, 220, 823, 836
SspMI	CTAG	2 cut(s)	546, 641
SstI	GAGCTC	1 cut(s)	667
StyD4I	CCNGG	4 cut(s)	200, 234, 692, 778
StyI	CCWWGG	1 cut(s)	931
TaaI	ACNGT	1 cut(s)	319
TaiI	ACGT	3 cut(s)	573, 714, 765
TaqI	TCGA	3 cut(s)	312, 567, 717
TasI	AATT	1 cut(s)	481
TauI	GCSGC	2 cut(s)	222, 839
TfiI	GAWTC	1 cut(s)	104
Tru1I	TTAA	2 cut(s)	653, 851
Tru9I	TTAA	2 cut(s)	653, 851
TscAI	CASTG	5 cut(s)	273, 404, 466, 683, 867
TseFI	GTSAC	1 cut(s)	319
TseI	GCWGC	1 cut(s)	469
Tsp45I	GTSAC	1 cut(s)	319
TspDTI	ATGAA	8 cut(s)	17, 73, 416, 662, 690, 870, 902, 953
TspRI	CASTG	5 cut(s)	273, 404, 466, 683, 867
Tth111I	GACNNNGTC	1 cut(s)	563
Van91I	CCANNNNNTGG	1 cut(s)	963
VpaK11BI	GGWCC	3 cut(s)	160, 452, 776
XagI	CCTNNNNNAGG	1 cut(s)	533
XbaI	TCTAGA	1 cut(s)	640
XceI	RCATGY	1 cut(s)	331
XcmI	CCANNNNNNNNNTGG	1 cut(s)	938
XmiI	GTMKAC	1 cut(s)	567
XspI	CTAG	2 cut(s)	546, 641
ZraI	GACGTC	1 cut(s)	571

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.