Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004661

Physical Location & Seq

Genomic Coordinates Reverse (-)

6854686 .. 6857425

Gene Structure 2740 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1056200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1641 bp

ATGACGTTAACTCTCCGTTCTTCAGCTTCGTTCATCAATCTTAAAGACCACAAGGGCCTGAAAACCTCCGATGACTCCCCCGGCACGATATGCTTTGCACAAATCAAGCCATCATGCCGTCTCCGGGCAAAGAGTTCAATCCAAGAAACACAGCTCTTGCATGAGAAGAACTTAATGACTATCTCCGATGGAAGGAGAGAGTTGCTCCACGCACTCCCAAATACTGCTCACAGCAGCACTGACAACAAGGTGCCTGTGTATGTGATGCTCCCGCTCGACACGGTGAGTCATGGAGGGCATTTGAATAAGCCGAGAGCAATGAATGCGAGTTTGATGGCCCTGAAGCAAGCAGGTGTTGAAGGAGTTATGGTGGATGCGTGGTGGGGATTGGTGGAGAAGGACGGACCTTCCAAGTACAATTGGGAAGGGTATGCTGAGCTTGTGCAGATGGTGCAAAAGCATGGCATGAAGATCCAAATCGTCATGTCTTTTCATCAGTGTGGAGGAAATGTTGGAGACTCATGCAGCATTCCTCTACCTCCATGGGTGCTTGAAGAAATCAGCAAGAATCCTGACCTTGTTTACACAGACAAATCAGGCAGGAGGAATCCTGAGTACATATCCTTGGGATGCGATTCATTGCCTGTTCTCAGAGGAAGAACTCCCATTCAGGTCTACACCGATTACATGAGGAGCTTCCGCGACAGATTCATTGACTACTTGGGCGATGTTATTGTGGAAATTCAAGTAGGCATGGGTCCTTGTGGGGAGCTCAGATATCCAGCTTATCCAGAAAGCAACGGAACTTGGAGGTTTCCTGGAATTGGAGAATTCCAATGCTATGACAAGTACATGAGAGCTTCCCTGGAAGCATCAGCAGAGGCACTGGGGAAGAGAGATTGGGGAAGAAGTGGACCACATGATTCCGGCCACTACAATCAATTTCCTGAAGACACAGGATTTTTCAAAAGAGATGGAACTTGGAACACTGAGTATGGACAGTTCTTCCTTGAATGGTACTCCGGAAAGCTACTAAGACACGGAGATAGAATACTGTCAGCTGCAAAAGGGGTATTCCAAGGATCTGGAGCTAAATTATCTGGAAAGATTGCTGGGATTCACTGGCACTACAGATCAAGATCACATGCGGCTGAATTAACTGCGGGCTACTACAACACTAGACACAGAGATGGTTACCTACCAACTGCCAAAATGTTCAGCAAAAACATGGTTGTATTAAACTTCACCTGCATGGAAATGAAAGATAGAGAACAGCCAGAACATGCAAATTGCTCACCAGAAGGTCTAGTGAGGCAGGTAAAGATGGCAACCAAGAGAGCTGGAATTGACCTAGCAGGAGAGAATGCACTGGAGAGGTATGACACTGGTGCGTTTGCACAAGTTTTAGCAACAAGCAGATCAGATTCCGGTAATGCCTTAAGTGCATTTACATATCTCCGAATGAACAAGAGATTGTTCGATGGGGATAACTGGAGGAACATGGTTGAATTTGTGAGGAGCATGGCAGAAGGTGGCCGGAACAGACGTCTATCAGAGTGTGACTCCACCGGAAGTGACCTCTTTGTTCGTTTTATCAAAGAAAAGAAAGTTGAGAAAACAAAAGAAACTCTTCTCGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016052 GO:0016160 GO:0016161 GO:0016787 GO:0016798 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_14

Protein Analysis

547

Amino Acids

61.46

Weight (kDa)

8.8

Isoelectric Point (pI)

40.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_14	PF01373	87 - 504	1.3e-215	Glycosyl hydrolase family 14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17090
fragaria_vesca	FvH4_5g20800
malus_domestica	MD04G1056200.v1.1 MD06G1043900.v1.1 MD06G1044100.v1.1
prunus_persica	Prupe.5G051000_v2.0.a1
pyrus_communis	pycom04g04940 pycom06g03710 pycom06g03720
rosa_chinensis	RchiOBHm_Chr0c22g0500651 RchiOBHm_Chr7g0206601 RchiOBHm_Chr7g0207461 RchiOBHm_Chr7g0207721
rosa_laevigata	RLG00000003292
rosa_multiflora	Rmu_sc0007645.1_g000004 Rmu_sc0008509.1_g000004 Rmu_sc0008756.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251200 Rroxscaffold_3G00251330
rosa_rugosa	Rorug06G0115800 Rorug07G0099200
rosa_samantha	Rh7AG231100 Rh7AG231400 Rh7AG232400 Rh7BG227800 Rh7CG246000 Rh7DG238300 Rh7DG238600
rosa_wichuraiana	Rw0G021940 Rw7G019620 Rw7G019900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	341, 1256
AatII	GACGTC	1 cut(s)	1549
Acc36I	ACCTGC	3 cut(s)	341, 1256, 1306
AccB1I	GGYRCC	1 cut(s)	250
AccBSI	CCGCTC	1 cut(s)	274
AccI	GTMKAC	1 cut(s)	675
AccII	CGCG	1 cut(s)	702
AccIII	TCCGGA	1 cut(s)	1022
AciI	CCGC	4 cut(s)	272, 700, 1148, 1163
AclWI	GGATC	2 cut(s)	466, 1090
AcoI	YGGCCR	2 cut(s)	928, 1534
AcsI	RAATTY	3 cut(s)	741, 830, 1508
AcuI	CTGAAG	3 cut(s)	6, 362, 969
AcyI	GRCGYC	1 cut(s)	1546
AfaI	GTAC	4 cut(s)	416, 617, 851, 1019
AfiI	CCNNNNNNNGG	3 cut(s)	124, 192, 824
AflII	CTTAAG	1 cut(s)	1438
AgsI	TTSAA	8 cut(s)	138, 304, 359, 554, 746, 967, 1013, 1508
AjnI	CCWGG	2 cut(s)	817, 864
Alw21I	GWGCWC	1 cut(s)	774
Alw26I	GTCTC	2 cut(s)	125, 510
AlwI	GGATC	2 cut(s)	466, 1090
Aor13HI	TCCGGA	1 cut(s)	1022
AoxI	GGCC	4 cut(s)	55, 336, 928, 1534
ApeKI	GCWGC	3 cut(s)	234, 525, 1061
ApoI	RAATTY	3 cut(s)	741, 830, 1508
Asp700I	GAANNNNTTC	1 cut(s)	1629
AspS9I	GGNCC	5 cut(s)	55, 337, 404, 758, 914
AsuC2I	CCSGG	2 cut(s)	81, 125
AsuHPI	GGTGA	3 cut(s)	295, 1237, 1287
AvaII	GGWCC	3 cut(s)	404, 758, 914
BanI	GGYRCC	1 cut(s)	250
BanII	GRGCYC	1 cut(s)	774
BauI	CACGAG	1 cut(s)	1634
BbsI	GAAGAC	1 cut(s)	957
Bbv12I	GWGCWC	1 cut(s)	774
BbvI	GCAGC	3 cut(s)	246, 537, 1048
BccI	CCATC	8 cut(s)	118, 182, 328, 442, 968, 1184, 1318, 1475
BceAI	ACGGC	1 cut(s)	102
BciT130I	CCWGG	2 cut(s)	819, 866
BcnI	CCSGG	2 cut(s)	81, 125
BcoDI	GTCTC	2 cut(s)	125, 510
BfaI	CTAG	3 cut(s)	1179, 1307, 1352
BfmI	CTRYAG	1 cut(s)	1129
BfrI	CTTAAG	1 cut(s)	1438
BfuAI	ACCTGC	3 cut(s)	341, 1256, 1306
BisI	GCNGC	4 cut(s)	235, 526, 1062, 1149
BlpI	GCTNAGC	1 cut(s)	435
BlsI	GCNGC	4 cut(s)	236, 527, 1063, 1150
Bme1390I	CCNGG	4 cut(s)	81, 125, 819, 866
Bme18I	GGWCC	3 cut(s)	404, 758, 914
BmgT120I	GGNCC	5 cut(s)	55, 337, 404, 758, 914
BmiI	GGNNCC	2 cut(s)	252, 759
BmrFI	CCNGG	4 cut(s)	81, 125, 819, 866
BmrI	ACTGGG	1 cut(s)	896
BmsI	GCATC	4 cut(s)	255, 364, 620, 881
BmuI	ACTGGG	1 cut(s)	896
BpiI	GAAGAC	1 cut(s)	957
BplI	GAGNNNNNCTC	4 cut(s)	189, 221, 1547, 1579
BpmI	CTGGAG	3 cut(s)	1107, 1391, 1513
Bpu1102I	GCTNAGC	1 cut(s)	435
BpuMI	CCSGG	2 cut(s)	81, 125
BsaBI	GATNNNNATC	2 cut(s)	476, 1138
BsaHI	GRCGYC	1 cut(s)	1546
BsaJI	CCNNGG	5 cut(s)	79, 542, 624, 864, 1078
BsaWI	WCCGGW	3 cut(s)	1022, 1427, 1568
BsaXI	ACNNNNNCTCC	4 cut(s)	354, 384, 495, 525
Bsc4I	CCNNNNNNNGG	3 cut(s)	124, 192, 824
Bse1I	ACTGG	5 cut(s)	891, 1127, 1374, 1390, 1496
Bse3DI	GCAATG	2 cut(s)	324, 638
Bse8I	GATNNNNATC	2 cut(s)	476, 1138
BseAI	TCCGGA	1 cut(s)	1022
BseBI	CCWGG	2 cut(s)	819, 866
BseDI	CCNNGG	5 cut(s)	79, 542, 624, 864, 1078
BseGI	GGATG	2 cut(s)	379, 635
BseJI	GATNNNNATC	2 cut(s)	476, 1138
BseLI	CCNNNNNNNGG	3 cut(s)	124, 192, 824
BseMI	GCAATG	2 cut(s)	324, 638
BseMII	CTCAG	5 cut(s)	426, 603, 664, 787, 981
BseNI	ACTGG	5 cut(s)	891, 1127, 1374, 1390, 1496
BseRI	GAGGAG	2 cut(s)	706, 1531
BseXI	GCAGC	3 cut(s)	246, 537, 1048
BseYI	CCCAGC	1 cut(s)	1112
BsgI	GTGCAG	1 cut(s)	464
Bsh1236I	CGCG	1 cut(s)	702
BshFI	GGCC	4 cut(s)	57, 338, 930, 1536
BshNI	GGYRCC	1 cut(s)	250
BsiHKAI	GWGCWC	1 cut(s)	774
BsiSI	CCGG	7 cut(s)	81, 124, 927, 1023, 1428, 1537, 1569
BslI	CCNNNNNNNGG	3 cut(s)	124, 192, 824
BsmAI	GTCTC	2 cut(s)	125, 510
BsmBI	CGTCTC	1 cut(s)	125
BsmI	GAATGC	3 cut(s)	328, 528, 1369
BsnI	GGCC	4 cut(s)	57, 338, 930, 1536
Bsp1286I	GDGCHC	1 cut(s)	774
Bsp13I	TCCGGA	1 cut(s)	1022
Bsp143I	GATC	5 cut(s)	471, 1082, 1133, 1139, 1418
Bsp1720I	GCTNAGC	1 cut(s)	435
Bsp19I	CCATGG	1 cut(s)	542
BspACI	CCGC	4 cut(s)	272, 700, 1148, 1163
BspANI	GGCC	4 cut(s)	57, 338, 930, 1536
BspCNI	CTCAG	5 cut(s)	427, 604, 663, 786, 982
BspEI	TCCGGA	1 cut(s)	1022
BspFNI	CGCG	1 cut(s)	702
BspLI	GGNNCC	2 cut(s)	252, 759
BspMI	ACCTGC	3 cut(s)	341, 1256, 1306
BspPI	GGATC	2 cut(s)	466, 1090
BspT107I	GGYRCC	1 cut(s)	250
BspTI	CTTAAG	1 cut(s)	1438
BsrBI	CCGCTC	1 cut(s)	274
BsrDI	GCAATG	2 cut(s)	324, 638
BsrI	ACTGG	5 cut(s)	891, 1127, 1374, 1390, 1496
BssECI	CCNNGG	5 cut(s)	79, 542, 624, 864, 1078
BssMI	GATC	5 cut(s)	471, 1082, 1133, 1139, 1418
BssNI	GRCGYC	1 cut(s)	1546
BssSI	CACGAG	1 cut(s)	1634
BssT1I	CCWWGG	3 cut(s)	542, 624, 1078
Bst2BI	CACGAG	1 cut(s)	1634
Bst2UI	CCWGG	2 cut(s)	819, 866
Bst4CI	ACNGT	3 cut(s)	283, 1002, 1056
Bst6I	CTCTTC	2 cut(s)	887, 1635
BstACI	GRCGYC	1 cut(s)	1546
BstAFI	CTTAAG	1 cut(s)	1438
BstAPI	GCANNNNNTGC	3 cut(s)	90, 323, 451
BstC8I	GCNNGC	2 cut(s)	348, 1165
BstDEI	CTNAG	6 cut(s)	435, 612, 650, 773, 990, 1034
BstDSI	CCRYGG	1 cut(s)	542
BstEII	GGTNACC	1 cut(s)	1193
BstF5I	GGATG	2 cut(s)	379, 635
BstFNI	CGCG	1 cut(s)	702
BstKTI	GATC	5 cut(s)	474, 1085, 1136, 1142, 1421
BstMAI	GTCTC	2 cut(s)	125, 510
BstMBI	GATC	5 cut(s)	471, 1082, 1133, 1139, 1418
BstMWI	GCNNNNNNNGC	4 cut(s)	90, 323, 451, 1442
BstNI	CCWGG	2 cut(s)	819, 866
BstNSI	RCATGY	2 cut(s)	1148, 1286
BstPI	GGTNACC	1 cut(s)	1193
BstSCI	CCNGG	4 cut(s)	79, 123, 817, 864
BstSFI	CTRYAG	1 cut(s)	1129
BstUI	CGCG	1 cut(s)	702
BstV1I	GCAGC	3 cut(s)	246, 537, 1048
BstV2I	GAAGAC	1 cut(s)	957
BstX2I	RGATCY	2 cut(s)	471, 1082
BstXI	CCANNNNNNTGG	1 cut(s)	1085
BstYI	RGATCY	2 cut(s)	471, 1082
BsuRI	GGCC	4 cut(s)	57, 338, 930, 1536
BtgI	CCRYGG	1 cut(s)	542
BtgZI	GCGATG	1 cut(s)	741
BtsCI	GGATG	2 cut(s)	379, 635
BtsIMutI	CAGTG	7 cut(s)	237, 503, 884, 987, 1120, 1367, 1383
BveI	ACCTGC	3 cut(s)	341, 1256, 1306
Cac8I	GCNNGC	2 cut(s)	348, 1165
Cfr13I	GGNCC	5 cut(s)	55, 337, 404, 758, 914
Csp6I	GTAC	4 cut(s)	415, 616, 850, 1018
CviQI	GTAC	4 cut(s)	415, 616, 850, 1018
DdeI	CTNAG	6 cut(s)	435, 612, 650, 773, 990, 1034
DpnI	GATC	5 cut(s)	473, 1084, 1135, 1141, 1420
DpnII	GATC	5 cut(s)	471, 1082, 1133, 1139, 1418
EaeI	YGGCCR	2 cut(s)	928, 1534
Eam1104I	CTCTTC	2 cut(s)	887, 1635
EarI	CTCTTC	2 cut(s)	887, 1635
Ecl136II	GAGCTC	1 cut(s)	772
Eco130I	CCWWGG	3 cut(s)	542, 624, 1078
Eco24I	GRGCYC	1 cut(s)	774
Eco32I	GATATC	1 cut(s)	779
Eco47I	GGWCC	3 cut(s)	404, 758, 914
Eco53kI	GAGCTC	1 cut(s)	772
Eco57I	CTGAAG	3 cut(s)	6, 362, 969
Eco91I	GGTNACC	1 cut(s)	1193
EcoICRI	GAGCTC	1 cut(s)	772
EcoO109I	RGGNCCY	2 cut(s)	55, 758
EcoO65I	GGTNACC	1 cut(s)	1193
EcoRI	GAATTC	1 cut(s)	830
EcoRII	CCWGG	2 cut(s)	817, 864
EcoRV	GATATC	1 cut(s)	779
EcoT14I	CCWWGG	3 cut(s)	542, 624, 1078
EcoT38I	GRGCYC	1 cut(s)	774
ErhI	CCWWGG	3 cut(s)	542, 624, 1078
Esp3I	CGTCTC	1 cut(s)	125
FalI	AAGNNNNNCTT	1 cut(s)	1614
FauI	CCCGC	2 cut(s)	279, 1156
FblI	GTMKAC	1 cut(s)	675
Fnu4HI	GCNGC	4 cut(s)	235, 526, 1062, 1149
FokI	GGATG	2 cut(s)	386, 642
FriOI	GRGCYC	1 cut(s)	774
Fsp4HI	GCNGC	4 cut(s)	235, 526, 1062, 1149
FspBI	CTAG	3 cut(s)	1179, 1307, 1352
GluI	GCNGC	4 cut(s)	235, 526, 1062, 1149
GsaI	CCCAGC	1 cut(s)	1116
GsuI	CTGGAG	3 cut(s)	1107, 1391, 1513
HaeIII	GGCC	4 cut(s)	57, 338, 930, 1536
HapII	CCGG	7 cut(s)	81, 124, 927, 1023, 1428, 1537, 1569
Hin1I	GRCGYC	1 cut(s)	1546
HincII	GTYRAC	1 cut(s)	9
HindII	GTYRAC	1 cut(s)	9
HpaI	GTTAAC	1 cut(s)	9
HpaII	CCGG	7 cut(s)	81, 124, 927, 1023, 1428, 1537, 1569
HphI	GGTGA	3 cut(s)	295, 1237, 1287
Hpy166II	GTNNAC	4 cut(s)	9, 583, 676, 914
Hpy188I	TCNGA	7 cut(s)	70, 187, 653, 776, 1423, 1460, 1555
Hpy188III	TCNNGA	8 cut(s)	572, 611, 791, 947, 1023, 1086, 1101, 1137
Hpy8I	GTNNAC	4 cut(s)	9, 583, 676, 914
HpyAV	CCTTC	7 cut(s)	186, 353, 391, 417, 419, 1295, 1523
HpyCH4III	ACNGT	3 cut(s)	283, 1002, 1056
HpyCH4IV	ACGT	2 cut(s)	5, 1546
HpyF10VI	GCNNNNNNNGC	4 cut(s)	90, 323, 451, 1442
HpyF3I	CTNAG	6 cut(s)	435, 612, 650, 773, 990, 1034
HpySE526I	ACGT	2 cut(s)	5, 1546
Hsp92I	GRCGYC	1 cut(s)	1546
Kpn2I	TCCGGA	1 cut(s)	1022
KspAI	GTTAAC	1 cut(s)	9
Kzo9I	GATC	5 cut(s)	471, 1082, 1133, 1139, 1418
LmnI	GCTCC	6 cut(s)	210, 273, 693, 769, 1088, 1518
Lsp1109I	GCAGC	3 cut(s)	246, 537, 1048
LweI	GCATC	4 cut(s)	255, 364, 620, 881
MaeI	CTAG	3 cut(s)	1179, 1307, 1352
MaeII	ACGT	2 cut(s)	5, 1546
MaeIII	GTNAC	3 cut(s)	1193, 1559, 1574
MalI	GATC	5 cut(s)	473, 1084, 1135, 1141, 1420
MbiI	CCGCTC	1 cut(s)	274
MboI	GATC	5 cut(s)	471, 1082, 1133, 1139, 1418
MfeI	CAATTG	1 cut(s)	418
MflI	RGATCY	2 cut(s)	471, 1082
MhlI	GDGCHC	1 cut(s)	774
MlyI	GAGTC	4 cut(s)	68, 295, 512, 1556
MmeI	TCCRAC	1 cut(s)	493
MroI	TCCGGA	1 cut(s)	1022
MroXI	GAANNNNTTC	1 cut(s)	1629
MseI	TTAA	6 cut(s)	8, 42, 173, 1157, 1238, 1439
MslI	CAYNNNNRTG	4 cut(s)	498, 1188, 1250, 1256
MspA1I	CMGCKG	1 cut(s)	1061
MspCI	CTTAAG	1 cut(s)	1438
MspI	CCGG	7 cut(s)	81, 124, 927, 1023, 1428, 1537, 1569
MspR9I	CCNGG	4 cut(s)	81, 125, 819, 866
MunI	CAATTG	1 cut(s)	418
Mva1269I	GAATGC	3 cut(s)	328, 528, 1369
MvaI	CCWGG	2 cut(s)	819, 866
MvnI	CGCG	1 cut(s)	702
MwoI	GCNNNNNNNGC	4 cut(s)	90, 323, 451, 1442
NciI	CCSGG	2 cut(s)	81, 125
NcoI	CCATGG	1 cut(s)	542
NdeII	GATC	5 cut(s)	471, 1082, 1133, 1139, 1418
NlaIV	GGNNCC	2 cut(s)	252, 759
NmeAIII	GCCGAG	1 cut(s)	336
NmuCI	GTSAC	2 cut(s)	1559, 1574
NspI	RCATGY	2 cut(s)	1148, 1286
PaqCI	CACCTGC	2 cut(s)	341, 1256
PctI	GAATGC	3 cut(s)	328, 528, 1369
PdmI	GAANNNNTTC	1 cut(s)	1629
PfeI	GAWTC	7 cut(s)	568, 607, 635, 708, 923, 1117, 1424
PfoI	TCCNGGA	1 cut(s)	817
PkrI	GCNGC	4 cut(s)	236, 527, 1063, 1150
PleI	GAGTC	4 cut(s)	68, 294, 512, 1556
PpsI	GAGTC	4 cut(s)	68, 294, 512, 1556
PpuMI	RGGWCCY	1 cut(s)	758
Psp124BI	GAGCTC	1 cut(s)	774
Psp5II	RGGWCCY	1 cut(s)	758
Psp6I	CCWGG	2 cut(s)	817, 864
PspEI	GGTNACC	1 cut(s)	1193
PspFI	CCCAGC	1 cut(s)	1112
PspGI	CCWGG	2 cut(s)	817, 864
PspN4I	GGNNCC	2 cut(s)	252, 759
PspPI	GGNCC	5 cut(s)	55, 337, 404, 758, 914
PspPPI	RGGWCCY	1 cut(s)	758
PsrI	GAACNNNNNNTAC	2 cut(s)	986, 1018
PsuI	RGATCY	2 cut(s)	471, 1082
PvuII	CAGCTG	1 cut(s)	1061
RsaI	GTAC	4 cut(s)	416, 617, 851, 1019
RsaNI	GTAC	4 cut(s)	415, 616, 850, 1018
RseI	CAYNNNNRTG	4 cut(s)	498, 1188, 1250, 1256
SacI	GAGCTC	1 cut(s)	774
SaqAI	TTAA	6 cut(s)	8, 42, 173, 1157, 1238, 1439
SatI	GCNGC	4 cut(s)	235, 526, 1062, 1149
Sau3AI	GATC	5 cut(s)	471, 1082, 1133, 1139, 1418
Sau96I	GGNCC	5 cut(s)	55, 337, 404, 758, 914
SchI	GAGTC	4 cut(s)	68, 295, 512, 1556
ScrFI	CCNGG	4 cut(s)	81, 125, 819, 866
SduI	GDGCHC	1 cut(s)	774
SfaNI	GCATC	4 cut(s)	255, 364, 620, 881
SfcI	CTRYAG	1 cut(s)	1129
SinI	GGWCC	3 cut(s)	404, 758, 914
SmiMI	CAYNNNNRTG	4 cut(s)	498, 1188, 1250, 1256
SmlI	CTYRAG	1 cut(s)	1438
SmoI	CTYRAG	1 cut(s)	1438
SsiI	CCGC	4 cut(s)	272, 700, 1148, 1163
SspMI	CTAG	3 cut(s)	1179, 1307, 1352
SstI	GAGCTC	1 cut(s)	774
StyD4I	CCNGG	4 cut(s)	79, 123, 817, 864
StyI	CCWWGG	3 cut(s)	542, 624, 1078
TaaI	ACNGT	3 cut(s)	283, 1002, 1056
TaiI	ACGT	2 cut(s)	8, 1549
TaqI	TCGA	2 cut(s)	276, 1479
TatI	WGTACW	3 cut(s)	414, 615, 849
TauI	GCSGC	1 cut(s)	1151
TfiI	GAWTC	7 cut(s)	568, 607, 635, 708, 923, 1117, 1424
Tru1I	TTAA	6 cut(s)	8, 42, 173, 1157, 1238, 1439
Tru9I	TTAA	6 cut(s)	8, 42, 173, 1157, 1238, 1439
TscAI	CASTG	7 cut(s)	244, 503, 891, 994, 1127, 1374, 1390
TseFI	GTSAC	2 cut(s)	1559, 1574
TseI	GCWGC	3 cut(s)	234, 525, 1061
Tsp45I	GTSAC	2 cut(s)	1559, 1574
TspDTI	ATGAA	8 cut(s)	22, 335, 482, 482, 627, 700, 1274, 1478
TspGWI	ACGGA	4 cut(s)	5, 417, 816, 1056
TspRI	CASTG	7 cut(s)	244, 503, 891, 994, 1127, 1374, 1390
Vha464I	CTTAAG	1 cut(s)	1438
VpaK11BI	GGWCC	3 cut(s)	404, 758, 914
XapI	RAATTY	3 cut(s)	741, 830, 1508
XceI	RCATGY	2 cut(s)	1148, 1286
XmiI	GTMKAC	1 cut(s)	675
XmnI	GAANNNNTTC	1 cut(s)	1629
XspI	CTAG	3 cut(s)	1179, 1307, 1352
ZraI	GACGTC	1 cut(s)	1547

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.