Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004661

Physical Location & Seq

Genomic Coordinates Forward (+)

5935540 .. 5937213

Gene Structure 1674 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1044100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 831 bp

ATGAATGCGAGTTTGATGGCCCTGAAGCAAGCAGGGGTGGAAGGAGTTATGGTGGATGTGTGGTGGGGATTGGTGGAGGAGGACGGACCTTCCAAGTACAACTGGGAAGGGTATGCCGAGCTTGTGCAGATGGTGCAAAAGCACGGCATGAAGATCCAAGTCGTCATGTCTTTTCATCAGTGCGGAGGAAATGTTGGAGACTCATGCAGCATTCCTTTACCTCCATGGGTGCTTGAAGAAGTCAGCAAGAACCCTGACCTTGTTTACACAGACAAATCAGGCAGGAGGAATCCCGAGTACATATCCTTGGGATGTGATTCATTGCCTGTTCTCGGAGGAAGAACTCCCATTCAGGTCTACACCGATTACATGAGGAGCTTCCACGACAGATTCATTGACTACTTGGGCGACGTTATTGTGGAAATTCAAGTAGGCATGGGTCCTTGTGGGGCGCTCAGATATCCAGCTTATCCAGAAAGCAACGGAACTTGGAGGTTTCCTGGAATCGGGGAATTCCAATGTTATGACAAGCTTCCTTGGAAGCATCAGCAGAGGCATTGGGGAAGAAGTGGACCACATGATTCCGGCCAGTACAATCAATTTCCTGAAGACACCGGATTTTTCAAAAGAGATGGAACATGGAACACTGAGCATGGACAGTTCTTCTTTGAATGGTACTCCGGAAAGCTACTAAGACACGGAGATAGAATATTGTCAGCTGCGAAAGGAGTATTCCAAGGATCTGGAGCTAAACTATCCGGAAAGATTGCTGGGATTCACTGGCACTACGGATCAAGATCACATGCGGCTGAATTAACTGCAGGCTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0001101 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016052 GO:0016160 GO:0016161 GO:0016787 GO:0016798 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071704 GO:1901575 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_14

Protein Analysis

277

Amino Acids

31.08

Weight (kDa)

6.25

Isoelectric Point (pI)

31.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_14	PF01373	1 - 276	3.3e-136	Glycosyl hydrolase family 14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17090
fragaria_vesca	FvH4_5g20800
malus_domestica	MD04G1056200.v1.1 MD06G1043900.v1.1 MD06G1044100.v1.1
prunus_persica	Prupe.5G051000_v2.0.a1
pyrus_communis	pycom04g04940 pycom06g03710 pycom06g03720
rosa_chinensis	RchiOBHm_Chr0c22g0500651 RchiOBHm_Chr7g0206601 RchiOBHm_Chr7g0207461 RchiOBHm_Chr7g0207721
rosa_laevigata	RLG00000003292
rosa_multiflora	Rmu_sc0007645.1_g000004 Rmu_sc0008509.1_g000004 Rmu_sc0008756.1_g000004
rosa_roxburghii	Rroxscaffold_3G00251200 Rroxscaffold_3G00251330
rosa_rugosa	Rorug06G0115800 Rorug07G0099200
rosa_samantha	Rh7AG231100 Rh7AG231400 Rh7AG232400 Rh7BG227800 Rh7CG246000 Rh7DG238300 Rh7DG238600
rosa_wichuraiana	Rw0G021940 Rw7G019620 Rw7G019900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	357
AccIII	TCCGGA	2 cut(s)	680, 758
AciI	CCGC	2 cut(s)	183, 806
AclWI	GGATC	3 cut(s)	148, 748, 799
AcoI	YGGCCR	1 cut(s)	586
AcsI	RAATTY	2 cut(s)	423, 512
AcuI	CTGAAG	2 cut(s)	44, 627
AfaI	GTAC	4 cut(s)	98, 299, 593, 677
AfiI	CCNNNNNNNGG	2 cut(s)	332, 506
AgsI	TTSAA	4 cut(s)	236, 428, 625, 671
AjnI	CCWGG	1 cut(s)	499
AluBI	AGCT	7 cut(s)	121, 378, 467, 532, 688, 719, 749
AluI	AGCT	7 cut(s)	121, 378, 467, 532, 688, 719, 749
Alw26I	GTCTC	1 cut(s)	192
AlwI	GGATC	3 cut(s)	148, 748, 799
Ama87I	CYCGRG	1 cut(s)	293
Aor13HI	TCCGGA	2 cut(s)	680, 758
AoxI	GGCC	2 cut(s)	18, 586
ApeKI	GCWGC	2 cut(s)	207, 719
ApoI	RAATTY	2 cut(s)	423, 512
AspLEI	GCGC	1 cut(s)	454
AspS9I	GGNCC	4 cut(s)	19, 86, 440, 572
AvaI	CYCGRG	1 cut(s)	293
AvaII	GGWCC	3 cut(s)	86, 440, 572
BbsI	GAAGAC	1 cut(s)	615
BbvI	GCAGC	2 cut(s)	219, 706
BccI	CCATC	3 cut(s)	10, 124, 626
BceAI	ACGGC	1 cut(s)	160
BciT130I	CCWGG	1 cut(s)	501
BcoDI	GTCTC	1 cut(s)	192
BfmI	CTRYAG	1 cut(s)	819
BfoI	RGCGCY	1 cut(s)	455
BisI	GCNGC	3 cut(s)	208, 720, 807
BlsI	GCNGC	3 cut(s)	209, 721, 808
Bme1390I	CCNGG	1 cut(s)	501
Bme18I	GGWCC	3 cut(s)	86, 440, 572
BmeT110I	CYCGRG	1 cut(s)	293
BmgT120I	GGNCC	4 cut(s)	19, 86, 440, 572
BmiI	GGNNCC	1 cut(s)	441
BmrFI	CCNGG	1 cut(s)	501
BmrI	ACTGGG	1 cut(s)	112
BmsI	GCATC	1 cut(s)	553
BmuI	ACTGGG	1 cut(s)	112
BpiI	GAAGAC	1 cut(s)	615
BpmI	CTGGAG	1 cut(s)	765
BsaBI	GATNNNNATC	1 cut(s)	796
BsaJI	CCNNGG	4 cut(s)	224, 306, 536, 736
BsaWI	WCCGGW	3 cut(s)	614, 680, 758
BsaXI	ACNNNNNCTCC	4 cut(s)	36, 66, 177, 207
Bsc4I	CCNNNNNNNGG	2 cut(s)	332, 506
Bse1I	ACTGG	3 cut(s)	107, 589, 785
Bse3DI	GCAATG	1 cut(s)	320
Bse8I	GATNNNNATC	1 cut(s)	796
BseAI	TCCGGA	2 cut(s)	680, 758
BseBI	CCWGG	1 cut(s)	501
BseDI	CCNNGG	4 cut(s)	224, 306, 536, 736
BseGI	GGATG	2 cut(s)	61, 317
BseJI	GATNNNNATC	1 cut(s)	796
BseLI	CCNNNNNNNGG	2 cut(s)	332, 506
BseMI	GCAATG	1 cut(s)	320
BseMII	CTCAG	2 cut(s)	469, 639
BseNI	ACTGG	3 cut(s)	107, 589, 785
BseRI	GAGGAG	2 cut(s)	92, 388
BseXI	GCAGC	2 cut(s)	219, 706
BseYI	CCCAGC	1 cut(s)	770
BsgI	GTGCAG	1 cut(s)	146
BshFI	GGCC	2 cut(s)	20, 588
BsiHKCI	CYCGRG	1 cut(s)	293
BsiSI	CCGG	4 cut(s)	585, 615, 681, 759
BslI	CCNNNNNNNGG	2 cut(s)	332, 506
BsmAI	GTCTC	1 cut(s)	192
BsmI	GAATGC	2 cut(s)	10, 210
BsnI	GGCC	2 cut(s)	20, 588
BsoBI	CYCGRG	1 cut(s)	293
Bsp13I	TCCGGA	2 cut(s)	680, 758
Bsp143I	GATC	4 cut(s)	153, 740, 791, 797
Bsp19I	CCATGG	1 cut(s)	224
BspACI	CCGC	2 cut(s)	183, 806
BspANI	GGCC	2 cut(s)	20, 588
BspCNI	CTCAG	2 cut(s)	468, 640
BspEI	TCCGGA	2 cut(s)	680, 758
BspLI	GGNNCC	1 cut(s)	441
BspMAI	CTGCAG	1 cut(s)	823
BspPI	GGATC	3 cut(s)	148, 748, 799
BsrDI	GCAATG	1 cut(s)	320
BsrI	ACTGG	3 cut(s)	107, 589, 785
BssECI	CCNNGG	4 cut(s)	224, 306, 536, 736
BssMI	GATC	4 cut(s)	153, 740, 791, 797
BssT1I	CCWWGG	4 cut(s)	224, 306, 536, 736
Bst2UI	CCWGG	1 cut(s)	501
Bst4CI	ACNGT	1 cut(s)	660
BstAPI	GCANNNNNTGC	1 cut(s)	133
BstC8I	GCNNGC	2 cut(s)	30, 823
BstDEI	CTNAG	3 cut(s)	455, 648, 692
BstDSI	CCRYGG	1 cut(s)	224
BstF5I	GGATG	2 cut(s)	61, 317
BstH2I	RGCGCY	1 cut(s)	455
BstHHI	GCGC	1 cut(s)	454
BstKTI	GATC	4 cut(s)	156, 743, 794, 800
BstMAI	GTCTC	1 cut(s)	192
BstMBI	GATC	4 cut(s)	153, 740, 791, 797
BstMWI	GCNNNNNNNGC	1 cut(s)	133
BstNI	CCWGG	1 cut(s)	501
BstNSI	RCATGY	1 cut(s)	806
BstSCI	CCNGG	1 cut(s)	499
BstSFI	CTRYAG	1 cut(s)	819
BstV1I	GCAGC	2 cut(s)	219, 706
BstV2I	GAAGAC	1 cut(s)	615
BstX2I	RGATCY	2 cut(s)	153, 740
BstXI	CCANNNNNNTGG	1 cut(s)	743
BstYI	RGATCY	2 cut(s)	153, 740
BsuRI	GGCC	2 cut(s)	20, 588
BtgI	CCRYGG	1 cut(s)	224
BtsCI	GGATG	2 cut(s)	61, 317
BtsIMutI	CAGTG	3 cut(s)	185, 645, 778
Cac8I	GCNNGC	2 cut(s)	30, 823
CfoI	GCGC	1 cut(s)	454
Cfr13I	GGNCC	4 cut(s)	19, 86, 440, 572
Csp6I	GTAC	4 cut(s)	97, 298, 592, 676
CviQI	GTAC	4 cut(s)	97, 298, 592, 676
DdeI	CTNAG	3 cut(s)	455, 648, 692
DpnI	GATC	4 cut(s)	155, 742, 793, 799
DpnII	GATC	4 cut(s)	153, 740, 791, 797
EaeI	YGGCCR	1 cut(s)	586
Eco130I	CCWWGG	4 cut(s)	224, 306, 536, 736
Eco32I	GATATC	1 cut(s)	461
Eco47I	GGWCC	3 cut(s)	86, 440, 572
Eco57I	CTGAAG	2 cut(s)	44, 627
Eco88I	CYCGRG	1 cut(s)	293
EcoO109I	RGGNCCY	1 cut(s)	440
EcoRI	GAATTC	1 cut(s)	512
EcoRII	CCWGG	1 cut(s)	499
EcoRV	GATATC	1 cut(s)	461
EcoT14I	CCWWGG	4 cut(s)	224, 306, 536, 736
ErhI	CCWWGG	4 cut(s)	224, 306, 536, 736
FblI	GTMKAC	1 cut(s)	357
Fnu4HI	GCNGC	3 cut(s)	208, 720, 807
FokI	GGATG	2 cut(s)	68, 324
Fsp4HI	GCNGC	3 cut(s)	208, 720, 807
GlaI	GCGC	1 cut(s)	453
GluI	GCNGC	3 cut(s)	208, 720, 807
GsaI	CCCAGC	1 cut(s)	774
GsuI	CTGGAG	1 cut(s)	765
HaeII	RGCGCY	1 cut(s)	455
HaeIII	GGCC	2 cut(s)	20, 588
HapII	CCGG	4 cut(s)	585, 615, 681, 759
HhaI	GCGC	1 cut(s)	454
Hin6I	GCGC	1 cut(s)	452
HinP1I	GCGC	1 cut(s)	452
HindIII	AAGCTT	1 cut(s)	530
HinfI	GANTC	7 cut(s)	200, 289, 317, 390, 504, 581, 775
HpaII	CCGG	4 cut(s)	585, 615, 681, 759
Hpy166II	GTNNAC	3 cut(s)	265, 358, 572
Hpy188I	TCNGA	2 cut(s)	335, 458
Hpy188III	TCNNGA	7 cut(s)	293, 473, 605, 681, 744, 759, 795
Hpy8I	GTNNAC	3 cut(s)	265, 358, 572
Hpy99I	CGWCG	1 cut(s)	413
HpyAV	CCTTC	3 cut(s)	35, 99, 101
HpyCH4III	ACNGT	1 cut(s)	660
HpyCH4IV	ACGT	1 cut(s)	411
HpyCH4V	TGCA	4 cut(s)	127, 136, 207, 821
HpyF10VI	GCNNNNNNNGC	1 cut(s)	133
HpyF3I	CTNAG	3 cut(s)	455, 648, 692
HpySE526I	ACGT	1 cut(s)	411
HspAI	GCGC	1 cut(s)	452
Kpn2I	TCCGGA	2 cut(s)	680, 758
Kzo9I	GATC	4 cut(s)	153, 740, 791, 797
LmnI	GCTCC	2 cut(s)	375, 746
Lsp1109I	GCAGC	2 cut(s)	219, 706
LweI	GCATC	1 cut(s)	553
MaeII	ACGT	1 cut(s)	411
MalI	GATC	4 cut(s)	155, 742, 793, 799
MboI	GATC	4 cut(s)	153, 740, 791, 797
MboII	GAAGA	6 cut(s)	163, 248, 351, 576, 620, 655
MflI	RGATCY	2 cut(s)	153, 740
MluCI	AATT	4 cut(s)	423, 512, 599, 812
MlyI	GAGTC	1 cut(s)	194
MmeI	TCCRAC	1 cut(s)	175
MnlI	CCTC	9 cut(s)	70, 73, 179, 231, 279, 329, 366, 486, 546
MroI	TCCGGA	2 cut(s)	680, 758
MseI	TTAA	1 cut(s)	815
MspA1I	CMGCKG	1 cut(s)	719
MspI	CCGG	4 cut(s)	585, 615, 681, 759
MspR9I	CCNGG	1 cut(s)	501
Mva1269I	GAATGC	2 cut(s)	10, 210
MvaI	CCWGG	1 cut(s)	501
MwoI	GCNNNNNNNGC	1 cut(s)	133
NcoI	CCATGG	1 cut(s)	224
NdeII	GATC	4 cut(s)	153, 740, 791, 797
NlaIV	GGNNCC	1 cut(s)	441
NmeAIII	GCCGAG	1 cut(s)	142
NspI	RCATGY	1 cut(s)	806
PctI	GAATGC	2 cut(s)	10, 210
PfeI	GAWTC	6 cut(s)	289, 317, 390, 504, 581, 775
PfoI	TCCNGGA	1 cut(s)	499
PkrI	GCNGC	3 cut(s)	209, 721, 808
PleI	GAGTC	1 cut(s)	194
PpsI	GAGTC	1 cut(s)	194
PpuMI	RGGWCCY	1 cut(s)	440
Psp5II	RGGWCCY	1 cut(s)	440
Psp6I	CCWGG	1 cut(s)	499
PspFI	CCCAGC	1 cut(s)	770
PspGI	CCWGG	1 cut(s)	499
PspN4I	GGNNCC	1 cut(s)	441
PspPI	GGNCC	4 cut(s)	19, 86, 440, 572
PspPPI	RGGWCCY	1 cut(s)	440
PstI	CTGCAG	1 cut(s)	823
PsuI	RGATCY	2 cut(s)	153, 740
PvuII	CAGCTG	1 cut(s)	719
RsaI	GTAC	4 cut(s)	98, 299, 593, 677
RsaNI	GTAC	4 cut(s)	97, 298, 592, 676
SaqAI	TTAA	1 cut(s)	815
SatI	GCNGC	3 cut(s)	208, 720, 807
Sau3AI	GATC	4 cut(s)	153, 740, 791, 797
Sau96I	GGNCC	4 cut(s)	19, 86, 440, 572
SchI	GAGTC	1 cut(s)	194
ScrFI	CCNGG	1 cut(s)	501
SfaNI	GCATC	1 cut(s)	553
SfcI	CTRYAG	1 cut(s)	819
SinI	GGWCC	3 cut(s)	86, 440, 572
Sse9I	AATT	4 cut(s)	423, 512, 599, 812
SsiI	CCGC	2 cut(s)	183, 806
SspI	AATATT	1 cut(s)	711
StyD4I	CCNGG	1 cut(s)	499
StyI	CCWWGG	4 cut(s)	224, 306, 536, 736
TaaI	ACNGT	1 cut(s)	660
TaiI	ACGT	1 cut(s)	414
TasI	AATT	4 cut(s)	423, 512, 599, 812
TatI	WGTACW	3 cut(s)	96, 297, 591
TauI	GCSGC	1 cut(s)	809
TfiI	GAWTC	6 cut(s)	289, 317, 390, 504, 581, 775
Tru1I	TTAA	1 cut(s)	815
Tru9I	TTAA	1 cut(s)	815
TscAI	CASTG	3 cut(s)	185, 652, 785
TseI	GCWGC	2 cut(s)	207, 719
TspDTI	ATGAA	5 cut(s)	17, 164, 164, 309, 382
TspGWI	ACGGA	4 cut(s)	99, 498, 714, 804
TspRI	CASTG	3 cut(s)	185, 652, 785
VpaK11BI	GGWCC	3 cut(s)	86, 440, 572
XapI	RAATTY	2 cut(s)	423, 512
XceI	RCATGY	1 cut(s)	806
XmiI	GTMKAC	1 cut(s)	357

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.