RchiOBHm_Chr7g0207461

Beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004661
Physical Location & Seq
Reverse (-)
24788338 .. 24788881
544 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18565

Sequence Viewer

Length: 408 bp
ATGAATGCGAGTTTGATGGCTTTGAAGAATGCAGGAGTAGAAGGAGTTATGGTGGATGCTTGGTGGGGATTGGTCGAGAAAGACGGACCTTCCAAGTACCACTGGGAAGGGTATGCAGAGCTTGTGAACTTGGTCCAAAAGCATGGCTTGAAGATTCAAGTTGTCATGTATTCTTTCCATCAGTGTGGAGGAAATGTTGGTGACTCATGCAGTATTCCACTACCTCCATGGTTGCTTGAAGAGATAAGCAAGAACCCTGACCTTGTGTACACAGACAAATCTGGTAGGAGGAATCCTGAGTACATATCCTTGGGGTGTGATTCATCGCCTGTTTTCGGAGGAAGAACGCCCATCCAGGTTTATACCGACTACATGCAGAGCTTCCAAGACAGATTCAGAGATTACTAG

Protein Analysis

135

Amino Acids

15.24

Weight (kDa)

5.26

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 1 - 135 4.9e-71 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 3 cut(s) 98, 269, 302
AfiI CCNNNNNNNGG 1 cut(s) 335
AgsI TTSAA 4 cut(s) 25, 151, 158, 239
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 2 cut(s) 121, 381
AluI AGCT 2 cut(s) 121, 381
AspS9I GGNCC 2 cut(s) 86, 133
AsuHPI GGTGA 1 cut(s) 212
AvaII GGWCC 2 cut(s) 86, 133
BccI CCATC 3 cut(s) 10, 186, 359
BciT130I CCWGG 1 cut(s) 356
BfaI CTAG 1 cut(s) 406
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 2 cut(s) 86, 133
BmgT120I GGNCC 2 cut(s) 86, 133
BmrFI CCNGG 1 cut(s) 356
BmrI ACTGGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 46
BmuI ACTGGG 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 227, 309
BsaXI ACNNNNNCTCC 4 cut(s) 36, 66, 180, 210
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse1I ACTGG 1 cut(s) 107
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 2 cut(s) 227, 309
BseGI GGATG 2 cut(s) 61, 351
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 1 cut(s) 107
BslI CCNNNNNNNGG 1 cut(s) 335
BsmI GAATGC 2 cut(s) 10, 34
Bsp1407I TGTACA 1 cut(s) 267
Bsp19I CCATGG 1 cut(s) 227
BspCNI CTCAG 1 cut(s) 289
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 1 cut(s) 107
BssECI CCNNGG 2 cut(s) 227, 309
BssT1I CCWWGG 2 cut(s) 227, 309
Bst2UI CCWGG 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 297
BstDSI CCRYGG 1 cut(s) 227
BstF5I GGATG 2 cut(s) 61, 351
BstNI CCWGG 1 cut(s) 356
BstNSI RCATGY 1 cut(s) 376
BstSCI CCNGG 1 cut(s) 354
BstXI CCANNNNNNTGG 2 cut(s) 143, 185
BtgI CCRYGG 1 cut(s) 227
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 2 cut(s) 61, 351
BtsIMutI CAGTG 2 cut(s) 100, 188
Cfr13I GGNCC 2 cut(s) 86, 133
Csp6I GTAC 3 cut(s) 97, 268, 301
CviAII CATG 5 cut(s) 143, 166, 207, 228, 373
CviJI RGCY 4 cut(s) 20, 121, 147, 381
CviKI_1 RGCY 4 cut(s) 20, 121, 147, 381
CviQI GTAC 3 cut(s) 97, 268, 301
DdeI CTNAG 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco130I CCWWGG 2 cut(s) 227, 309
Eco47I GGWCC 2 cut(s) 86, 133
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 2 cut(s) 227, 309
ErhI CCWWGG 2 cut(s) 227, 309
FaeI CATG 5 cut(s) 146, 169, 210, 231, 376
FaiI YATR 9 cut(s) 50, 114, 144, 167, 208, 229, 305, 363, 374
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FatI CATG 5 cut(s) 142, 165, 206, 227, 372
FokI GGATG 2 cut(s) 68, 338
FspBI CTAG 1 cut(s) 406
Hin1II CATG 5 cut(s) 146, 169, 210, 231, 376
HinfI GANTC 5 cut(s) 154, 203, 292, 320, 393
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 3 cut(s) 127, 268, 270
Hpy188I TCNGA 2 cut(s) 338, 398
Hpy188III TCNNGA 2 cut(s) 76, 296
Hpy8I GTNNAC 3 cut(s) 127, 268, 270
HpyAV CCTTC 3 cut(s) 35, 99, 101
HpyCH4V TGCA 4 cut(s) 32, 116, 210, 376
HpyF3I CTNAG 1 cut(s) 297
Hsp92II CATG 5 cut(s) 146, 169, 210, 231, 376
LpnPI CCDG 8 cut(s) 18, 88, 267, 270, 309, 341, 342, 368
LweI GCATC 1 cut(s) 46
MaeI CTAG 1 cut(s) 406
MaeIII GTNAC 1 cut(s) 200
MboII GAAGA 4 cut(s) 37, 163, 251, 354
MlyI GAGTC 1 cut(s) 197
MnlI CCTC 4 cut(s) 182, 234, 282, 332
MslI CAYNNNNRTG 1 cut(s) 183
MspR9I CCNGG 1 cut(s) 356
Mva1269I GAATGC 2 cut(s) 10, 34
MvaI CCWGG 1 cut(s) 356
NcoI CCATGG 1 cut(s) 227
NlaIII CATG 5 cut(s) 146, 169, 210, 231, 376
NmuCI GTSAC 1 cut(s) 200
NspI RCATGY 1 cut(s) 376
PctI GAATGC 2 cut(s) 10, 34
PfeI GAWTC 4 cut(s) 154, 292, 320, 393
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspPI GGNCC 2 cut(s) 86, 133
RsaI GTAC 3 cut(s) 98, 269, 302
RsaNI GTAC 3 cut(s) 97, 268, 301
RseI CAYNNNNRTG 1 cut(s) 183
Sau96I GGNCC 2 cut(s) 86, 133
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 356
SetI ASST 6 cut(s) 91, 123, 226, 264, 360, 383
SfaNI GCATC 1 cut(s) 46
SinI GGWCC 2 cut(s) 86, 133
SmiMI CAYNNNNRTG 1 cut(s) 183
SspMI CTAG 1 cut(s) 406
StyD4I CCNGG 1 cut(s) 354
StyI CCWWGG 2 cut(s) 227, 309
TaqI TCGA 1 cut(s) 75
TatI WGTACW 2 cut(s) 267, 300
TfiI GAWTC 4 cut(s) 154, 292, 320, 393
TscAI CASTG 2 cut(s) 107, 188
TseFI GTSAC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 2 cut(s) 17, 312
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 2 cut(s) 107, 188
VpaK11BI GGWCC 2 cut(s) 86, 133
XceI RCATGY 1 cut(s) 376
XcmI CCANNNNNNNNNTGG 1 cut(s) 225
XspI CTAG 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.