MD04G1060200.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003639
Physical Location & Seq
Reverse (-)
7808879 .. 7809927
1049 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1060200.v1.1.491

Sequence Viewer

Length: 594 bp
ATGGCTCGAAAGAACGATGGGTCAGCAAAAAGGGTTATGAACAAAGGAGCATGGACAGCGGAGGAAGATAGAAAACTTGCAGAATATACTGAAATTCATGGTGCAAAGAGGTGGAAGACAGTTGCTTCAATAGCAGGTTTGAATCGTTGCGGGAAGAGTTGCAGGTTGAGATGGTTGAACTATTTGAGACCCAATATTAAGAGAGGCAACATTTCTGATGATGAAGAGGACTTGATAATTAGACTTCATAAACTACTAGGAAACAGGTGGTCCTTGATTGCCGGGAGACTTCCAGGCCGAACAGACAATGAAATAAAGAACTACTGGAATTCTCATTTGAGCAAAAAAATGAGTCAGAAGGAGAAAAAACCGCAGACTTCAACAGCCCAAGACACGACGACGAAGTCTGCTACTGCTACTGCTACTAAACCTTCCCATAAAGCATCAGACGATTGCGCTGATACGAACTTTGATGTGAATGAGTTCTTTGACTTCTCTGCCGAAGGTTCTTATGGGTTGGAGTGGGTGAACAAGTTTCTTGAGCTTGATGAAAACACCACGGCTAAACAAGAAAAGGTCAGCAGCATTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

198

Amino Acids

22.36

Weight (kDa)

9.28

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 15 - 62 3.2e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 18 - 77 1.3e-14 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 68 - 113 7.8e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 72 - 121 6.3e-09 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 403
Acc36I ACCTGC 2 cut(s) 125, 153
AciI CCGC 3 cut(s) 59, 150, 371
AcsI RAATTY 2 cut(s) 93, 328
AgsI TTSAA 4 cut(s) 129, 142, 178, 381
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 1 cut(s) 544
AluI AGCT 1 cut(s) 544
Alw26I GTCTC 2 cut(s) 181, 280
AoxI GGCC 1 cut(s) 295
ApeKI GCWGC 1 cut(s) 582
ApoI RAATTY 2 cut(s) 93, 328
Asp700I GAANNNNTTC 1 cut(s) 482
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 1 cut(s) 270
AsuC2I CCSGG 1 cut(s) 283
AsuHPI GGTGA 1 cut(s) 538
AvaII GGWCC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 122
BccI CCATC 2 cut(s) 11, 165
BceAI ACGGC 1 cut(s) 576
BciT130I CCWGG 1 cut(s) 294
BcnI CCSGG 1 cut(s) 283
BcoDI GTCTC 2 cut(s) 181, 280
BfaI CTAG 1 cut(s) 257
BfuAI ACCTGC 2 cut(s) 125, 153
BisI GCNGC 1 cut(s) 583
BlsI GCNGC 1 cut(s) 584
Bme1390I CCNGG 2 cut(s) 283, 294
Bme18I GGWCC 1 cut(s) 270
BmgT120I GGNCC 1 cut(s) 270
BmrFI CCNGG 2 cut(s) 283, 294
BmsI GCATC 1 cut(s) 452
BpiI GAAGAC 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 560
BpuMI CCSGG 1 cut(s) 283
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 1 cut(s) 558
Bse1I ACTGG 1 cut(s) 329
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 558
BseNI ACTGG 1 cut(s) 329
BshFI GGCC 1 cut(s) 297
BsiSI CCGG 1 cut(s) 282
BsmAI GTCTC 2 cut(s) 181, 280
BsnI GGCC 1 cut(s) 297
Bso31I GGTCTC 1 cut(s) 181
BspACI CCGC 3 cut(s) 59, 150, 371
BspANI GGCC 1 cut(s) 297
BspMI ACCTGC 2 cut(s) 125, 153
BspTNI GGTCTC 1 cut(s) 181
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 1 cut(s) 558
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 1 cut(s) 121
Bst6I CTCTTC 2 cut(s) 149, 219
BstDSI CCRYGG 1 cut(s) 558
BstHHI GCGC 1 cut(s) 458
BstMAI GTCTC 2 cut(s) 181, 280
BstMWI GCNNNNNNNGC 2 cut(s) 56, 131
BstNI CCWGG 1 cut(s) 294
BstSCI CCNGG 2 cut(s) 281, 292
BstV2I GAAGAC 1 cut(s) 122
BsuRI GGCC 1 cut(s) 297
BtgI CCRYGG 1 cut(s) 558
BveI ACCTGC 2 cut(s) 125, 153
CfoI GCGC 1 cut(s) 458
Cfr13I GGNCC 1 cut(s) 270
CviAII CATG 2 cut(s) 51, 98
CviJI RGCY 5 cut(s) 5, 297, 386, 544, 563
CviKI_1 RGCY 5 cut(s) 5, 297, 386, 544, 563
DrdI GACNNNNNNGTC 1 cut(s) 403
DseDI GACNNNNNNGTC 1 cut(s) 403
Eam1104I CTCTTC 2 cut(s) 149, 219
EarI CTCTTC 2 cut(s) 149, 219
Eco31I GGTCTC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 270
EcoRI GAATTC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 292
FaeI CATG 2 cut(s) 54, 101
FaiI YATR 7 cut(s) 38, 52, 87, 99, 249, 438, 513
FatI CATG 2 cut(s) 50, 97
FauI CCCGC 1 cut(s) 143
Fnu4HI GCNGC 1 cut(s) 583
Fsp4HI GCNGC 1 cut(s) 583
FspBI CTAG 1 cut(s) 257
GlaI GCGC 1 cut(s) 457
GluI GCNGC 1 cut(s) 583
HaeIII GGCC 1 cut(s) 297
HapII CCGG 1 cut(s) 282
HhaI GCGC 1 cut(s) 458
Hin1II CATG 2 cut(s) 54, 101
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HinfI GANTC 2 cut(s) 142, 352
HpaII CCGG 1 cut(s) 282
HphI GGTGA 1 cut(s) 538
Hpy166II GTNNAC 1 cut(s) 529
Hpy188I TCNGA 3 cut(s) 217, 357, 448
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 1 cut(s) 529
Hpy99I CGWCG 2 cut(s) 400, 403
HpyAV CCTTC 3 cut(s) 352, 441, 497
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4V TGCA 3 cut(s) 80, 104, 162
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 131
Hsp92II CATG 2 cut(s) 54, 101
HspAI GCGC 1 cut(s) 456
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 7 cut(s) 120, 148, 250, 279, 295, 306, 310
LweI GCATC 1 cut(s) 452
MaeI CTAG 1 cut(s) 257
MboII GAAGA 4 cut(s) 77, 127, 166, 236
MluCI AATT 3 cut(s) 93, 237, 328
MlyI GAGTC 1 cut(s) 361
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 4 cut(s) 55, 102, 197, 220
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 2 cut(s) 283, 294
MvaI CCWGG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 56, 131
NciI CCSGG 1 cut(s) 283
NlaIII CATG 2 cut(s) 54, 101
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 1 cut(s) 142
PflFI GACNNNGTC 1 cut(s) 403
PkrI GCNGC 1 cut(s) 584
PleI GAGTC 1 cut(s) 360
PpsI GAGTC 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 292
PspPI GGNCC 1 cut(s) 270
PsyI GACNNNGTC 1 cut(s) 403
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 1 cut(s) 583
Sau96I GGNCC 1 cut(s) 270
SchI GAGTC 1 cut(s) 361
ScrFI CCNGG 2 cut(s) 283, 294
SetI ASST 8 cut(s) 113, 139, 167, 269, 433, 508, 546, 579
SfaNI GCATC 1 cut(s) 452
SinI GGWCC 1 cut(s) 270
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 3 cut(s) 93, 237, 328
SsiI CCGC 3 cut(s) 59, 150, 371
SspI AATATT 1 cut(s) 196
SspMI CTAG 1 cut(s) 257
StyD4I CCNGG 2 cut(s) 281, 292
TaaI ACNGT 1 cut(s) 121
TaqI TCGA 1 cut(s) 7
TasI AATT 3 cut(s) 93, 237, 328
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TseI GCWGC 1 cut(s) 582
TspDTI ATGAA 6 cut(s) 53, 86, 236, 237, 324, 564
Tth111I GACNNNGTC 1 cut(s) 403
VpaK11BI GGWCC 1 cut(s) 270
XapI RAATTY 2 cut(s) 93, 328
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.