MD04G1061000.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007755
Physical Location & Seq
Forward (+)
8046050 .. 8047641
1592 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1061000.v1.1.491

Sequence Viewer

Length: 696 bp
ATGGCGACAGCGCCAGTGAAGCCGCCGCTGCACAACTTCCCGCTCACATTCTTGAAATGGGGGACTAAGAACGCCACCGCCAACAACCACAACCGCTACCGCCGACCCGCCTCCGCTGAACCAGCCTCCGAGCCCGACTCCGAATCCGACCGCCACAACCGGGTCGGGTCCTCCCGGGCCGATCGCCGCCGATTATCGCTGATCTCGTGCTCGGAAAACAAGCGCCGCAGGAGCGAGGAGAGGGAGAGCGATCAAGAGGAGGAGGAAGCTGAGGTGCTACTTCAGAAGCCCTGGAATTTGAGGCCCAGGAGACCTCCCGCGACGGCGTCGTTTCAAAAGGCGAGTGGCCCTAACGCCGCGGTTGGTGCGAACCGGGAAGGGCAGGAACCTGAAGGGCCGAACAGAAGTCAGAGCGAGATGATGCAGCAGCAGCAGCCGAAGTCGATGAGGTTGAGGGGTTTGGCGGAGGGGCAAAGTGTGGAGAAGAAGAAGGAGAAGAGCAAGTTCTGGATCGCTTTGTCGAAGGAGGAGATTGAAGAGGACGTGTTCGTGATGACCGGGTCGAGGCCCTCCAGAAGGCCCAAGAAGCGACCCAAGAATGTTCAGAAGCAGCTCGATAGTATCTTTCCTGGGTTGTGGCTGGTTGGTGTTACTGCTGATGCTTATAAGTCTGCTGATTCTCCATGTAAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

232

Amino Acids

26.2

Weight (kDa)

9.96

Isoelectric Point (pI)

90.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 173 - 222 2e-28 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AccBSI CCGCTC 1 cut(s) 43
AccII CGCG 2 cut(s) 320, 359
AclWI GGATC 1 cut(s) 518
AcsI RAATTY 1 cut(s) 295
AcuI CTGAAG 2 cut(s) 266, 411
AcyI GRCGYC 1 cut(s) 326
AfiI CCNNNNNNNGG 3 cut(s) 160, 564, 576
AflIII ACRYGT 1 cut(s) 543
AgsI TTSAA 3 cut(s) 55, 335, 536
AjiI CACGTC 1 cut(s) 544
AjnI CCWGG 3 cut(s) 290, 305, 628
AluBI AGCT 2 cut(s) 269, 613
AluI AGCT 2 cut(s) 269, 613
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 304
AlwI GGATC 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 6 cut(s) 177, 302, 346, 395, 566, 578
ApeKI GCWGC 6 cut(s) 28, 424, 427, 430, 433, 610
ApoI RAATTY 1 cut(s) 295
AspLEI GCGC 2 cut(s) 13, 225
AspS9I GGNCC 7 cut(s) 168, 177, 303, 347, 395, 567, 579
AsuC2I CCSGG 5 cut(s) 161, 175, 176, 374, 559
AvaI CYCGRG 1 cut(s) 174
AvaII GGWCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 135
BauI CACGAG 1 cut(s) 205
Bbv12I GWGCWC 1 cut(s) 212
BbvCI CCTCAGC 1 cut(s) 270
BbvI GCAGC 6 cut(s) 15, 436, 439, 442, 445, 622
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 3 cut(s) 292, 307, 630
BcnI CCSGG 5 cut(s) 161, 175, 176, 374, 559
BcoDI GTCTC 1 cut(s) 304
BfoI RGCGCY 2 cut(s) 14, 226
Bme1390I CCNGG 8 cut(s) 161, 175, 176, 292, 307, 374, 559, 630
Bme18I GGWCC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 174
BmgBI CACGTC 1 cut(s) 544
BmgT120I GGNCC 7 cut(s) 168, 177, 303, 347, 395, 567, 579
BmiI GGNNCC 2 cut(s) 169, 387
BmrFI CCNGG 8 cut(s) 161, 175, 176, 292, 307, 374, 559, 630
BmsI GCATC 2 cut(s) 411, 649
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpmI CTGGAG 1 cut(s) 556
Bpu10I CCTNAGC 1 cut(s) 270
BpuMI CCSGG 5 cut(s) 161, 175, 176, 374, 559
BsaHI GRCGYC 1 cut(s) 326
BsaI GGTCTC 1 cut(s) 304
BsaJI CCNNGG 5 cut(s) 174, 290, 305, 357, 629
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 564, 576
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 3 cut(s) 292, 307, 630
BseDI CCNNGG 5 cut(s) 174, 290, 305, 357, 629
BseLI CCNNNNNNNGG 3 cut(s) 160, 564, 576
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 4 cut(s) 251, 272, 275, 542
BseXI GCAGC 6 cut(s) 15, 436, 439, 442, 445, 622
BsgI GTGCAG 1 cut(s) 14
Bsh1236I CGCG 2 cut(s) 320, 359
Bsh1285I CGRYCG 2 cut(s) 151, 184
BshFI GGCC 6 cut(s) 179, 304, 348, 397, 568, 580
BsiEI CGRYCG 2 cut(s) 151, 184
BsiHKAI GWGCWC 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 4 cut(s) 160, 175, 373, 558
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 3 cut(s) 160, 564, 576
BsmAI GTCTC 1 cut(s) 304
BsmFI GGGAC 1 cut(s) 76
BsnI GGCC 6 cut(s) 179, 304, 348, 397, 568, 580
Bso31I GGTCTC 1 cut(s) 304
BsoBI CYCGRG 1 cut(s) 174
Bsp1286I GDGCHC 2 cut(s) 135, 212
Bsp143I GATC 4 cut(s) 181, 201, 250, 510
BspANI GGCC 6 cut(s) 179, 304, 348, 397, 568, 580
BspCNI CTCAG 1 cut(s) 262
BspFNI CGCG 2 cut(s) 320, 359
BspLI GGNNCC 2 cut(s) 169, 387
BspPI GGATC 1 cut(s) 518
BspQI GCTCTTC 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 304
BsrBI CCGCTC 1 cut(s) 43
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 5 cut(s) 174, 290, 305, 357, 629
BssMI GATC 4 cut(s) 181, 201, 250, 510
BssNI GRCGYC 1 cut(s) 326
BssSI CACGAG 1 cut(s) 205
Bst2BI CACGAG 1 cut(s) 205
Bst2UI CCWGG 3 cut(s) 292, 307, 630
Bst6I CTCTTC 2 cut(s) 491, 531
BstACI GRCGYC 1 cut(s) 326
BstDEI CTNAG 2 cut(s) 66, 270
BstDSI CCRYGG 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 574
BstFNI CGCG 2 cut(s) 320, 359
BstH2I RGCGCY 2 cut(s) 14, 226
BstHHI GCGC 2 cut(s) 13, 225
BstKTI GATC 4 cut(s) 184, 204, 253, 513
BstMAI GTCTC 1 cut(s) 304
BstMBI GATC 4 cut(s) 181, 201, 250, 510
BstMCI CGRYCG 2 cut(s) 151, 184
BstMWI GCNNNNNNNGC 8 cut(s) 19, 28, 122, 231, 365, 430, 433, 586
BstNI CCWGG 3 cut(s) 292, 307, 630
BstSCI CCNGG 8 cut(s) 159, 173, 174, 290, 305, 372, 557, 628
BstUI CGCG 2 cut(s) 320, 359
BstV1I GCAGC 6 cut(s) 15, 436, 439, 442, 445, 622
BsuRI GGCC 6 cut(s) 179, 304, 348, 397, 568, 580
BtgI CCRYGG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 21
CfoI GCGC 2 cut(s) 13, 225
Cfr13I GGNCC 7 cut(s) 168, 177, 303, 347, 395, 567, 579
Cfr42I CCGCGG 1 cut(s) 360
Cfr9I CCCGGG 1 cut(s) 174
CseI GACGC 1 cut(s) 315
CviAII CATG 1 cut(s) 684
DdeI CTNAG 2 cut(s) 66, 270
DpnI GATC 4 cut(s) 183, 203, 252, 512
DpnII GATC 4 cut(s) 181, 201, 250, 510
Eam1104I CTCTTC 2 cut(s) 491, 531
EarI CTCTTC 2 cut(s) 491, 531
EciI GGCGGA 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 168
Eco57I CTGAAG 2 cut(s) 266, 411
Eco88I CYCGRG 1 cut(s) 174
EcoNI CCTNNNNNAGG 1 cut(s) 574
EcoO109I RGGNCCY 2 cut(s) 168, 567
EcoRII CCWGG 3 cut(s) 290, 305, 628
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 1 cut(s) 687
FaiI YATR 2 cut(s) 666, 685
FaqI GGGAC 1 cut(s) 76
FatI CATG 1 cut(s) 683
FauI CCCGC 3 cut(s) 48, 115, 325
FriOI GRGCYC 1 cut(s) 135
GlaI GCGC 2 cut(s) 12, 224
GsuI CTGGAG 1 cut(s) 556
HaeII RGCGCY 2 cut(s) 14, 226
HaeIII GGCC 6 cut(s) 179, 304, 348, 397, 568, 580
HapII CCGG 4 cut(s) 160, 175, 373, 558
HgaI GACGC 1 cut(s) 315
HhaI GCGC 2 cut(s) 13, 225
Hin1I GRCGYC 1 cut(s) 326
Hin1II CATG 1 cut(s) 687
Hin6I GCGC 2 cut(s) 11, 223
HinP1I GCGC 2 cut(s) 11, 223
HinfI GANTC 3 cut(s) 137, 143, 677
HpaII CCGG 4 cut(s) 160, 175, 373, 558
Hpy188I TCNGA 7 cut(s) 130, 142, 148, 214, 285, 411, 606
Hpy188III TCNNGA 5 cut(s) 52, 254, 508, 550, 573
Hpy99I CGWCG 2 cut(s) 325, 331
HpyAV CCTTC 5 cut(s) 371, 386, 484, 517, 570
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 2 cut(s) 31, 424
HpyF10VI GCNNNNNNNGC 8 cut(s) 19, 28, 122, 231, 365, 430, 433, 586
HpyF3I CTNAG 2 cut(s) 66, 270
HpySE526I ACGT 1 cut(s) 543
Hsp92I GRCGYC 1 cut(s) 326
Hsp92II CATG 1 cut(s) 687
HspAI GCGC 2 cut(s) 11, 223
KspI CCGCGG 1 cut(s) 360
Kzo9I GATC 4 cut(s) 181, 201, 250, 510
LguI GCTCTTC 1 cut(s) 491
LmnI GCTCC 1 cut(s) 231
Lsp1109I GCAGC 6 cut(s) 15, 436, 439, 442, 445, 622
LweI GCATC 2 cut(s) 411, 649
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 1 cut(s) 649
MalI GATC 4 cut(s) 183, 203, 252, 512
MbiI CCGCTC 1 cut(s) 43
MboI GATC 4 cut(s) 181, 201, 250, 510
MboII GAAGA 4 cut(s) 496, 499, 508, 548
MhlI GDGCHC 2 cut(s) 135, 212
MluCI AATT 1 cut(s) 295
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 171
MspA1I CMGCKG 3 cut(s) 28, 116, 359
MspI CCGG 4 cut(s) 160, 175, 373, 558
MspR9I CCNGG 8 cut(s) 161, 175, 176, 292, 307, 374, 559, 630
MvaI CCWGG 3 cut(s) 292, 307, 630
MvnI CGCG 2 cut(s) 320, 359
MwoI GCNNNNNNNGC 8 cut(s) 19, 28, 122, 231, 365, 430, 433, 586
NciI CCSGG 5 cut(s) 161, 175, 176, 374, 559
NdeII GATC 4 cut(s) 181, 201, 250, 510
NlaIII CATG 1 cut(s) 687
NlaIV GGNNCC 2 cut(s) 169, 387
PciSI GCTCTTC 1 cut(s) 491
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PfeI GAWTC 2 cut(s) 143, 677
PflFI GACNNNGTC 2 cut(s) 325, 559
Ple19I CGATCG 1 cut(s) 184
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PpuMI RGGWCCY 1 cut(s) 168
PsiI TTATAA 1 cut(s) 666
Psp5II RGGWCCY 1 cut(s) 168
Psp6I CCWGG 3 cut(s) 290, 305, 628
PspGI CCWGG 3 cut(s) 290, 305, 628
PspN4I GGNNCC 2 cut(s) 169, 387
PspPI GGNCC 7 cut(s) 168, 177, 303, 347, 395, 567, 579
PspPPI RGGWCCY 1 cut(s) 168
PsyI GACNNNGTC 2 cut(s) 325, 559
PvuI CGATCG 1 cut(s) 184
SacII CCGCGG 1 cut(s) 360
SapI GCTCTTC 1 cut(s) 491
Sau3AI GATC 4 cut(s) 181, 201, 250, 510
Sau96I GGNCC 7 cut(s) 168, 177, 303, 347, 395, 567, 579
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 8 cut(s) 161, 175, 176, 292, 307, 374, 559, 630
SduI GDGCHC 2 cut(s) 135, 212
SetI ASST 8 cut(s) 271, 276, 316, 391, 452, 546, 615, 695
SfaNI GCATC 2 cut(s) 411, 649
Sfr303I CCGCGG 1 cut(s) 360
SgrBI CCGCGG 1 cut(s) 360
SinI GGWCC 1 cut(s) 168
SmaI CCCGGG 1 cut(s) 176
Sse9I AATT 1 cut(s) 295
StyD4I CCNGG 8 cut(s) 159, 173, 174, 290, 305, 372, 557, 628
TaiI ACGT 1 cut(s) 546
TaqI TCGA 4 cut(s) 443, 521, 563, 615
TasI AATT 1 cut(s) 295
TauI GCSGC 5 cut(s) 25, 28, 189, 228, 359
TfiI GAWTC 2 cut(s) 143, 677
TscAI CASTG 1 cut(s) 21
TseI GCWGC 6 cut(s) 28, 424, 427, 430, 433, 610
TspMI CCCGGG 1 cut(s) 174
TspRI CASTG 1 cut(s) 21
Tth111I GACNNNGTC 2 cut(s) 325, 559
VpaK11BI GGWCC 1 cut(s) 168
XagI CCTNNNNNAGG 1 cut(s) 574
XapI RAATTY 1 cut(s) 295
XmaI CCCGGG 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.