MD06G1054500.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007755
Physical Location & Seq
Forward (+)
7951694 .. 7954558
2865 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1054500.v1.1.491

Sequence Viewer

Length: 876 bp
ATGGCGACAGCGCCAGTGAAGTCGCCGCTGCACAACTTCCCATTCACATTCTTGAAATGGGGGACTAAGAACGCCACTGCCAACAACCACAACCGCAACCGCCGACCCGCCTCCTCCGAACCGGCCTCCGACCCAGACTCCGAACCAGACCGCTATAACCACGTCGGGTCCTCCCGCGCCGATCGCTGCCGATTATCGTTGATCCCGTGCTCGGAAAACGAGCGTCGCAGGAGCGAGGAGCGGGAGAGCGATCAAGAAGAGGAGGAAGCTGAGGTGCTACTTCAGAAGCCATGGAATTTGAGGCCCAGAAGAGCTCTGGCGACGGCGTCGTTTCAAAAGGCGAGTGGCCCTAACGCTGCGGCTGCTGCAAACCGGGAAGCGCAGGAACCGGAGGGGCCAAACCGGAGTCAGAGCGAAATGATGCAGCAGCAGCAGCCGAAGTCGATGAGGTTGAGGGGTCTGGCGGAGGGGCAGAGTGTGGAGAAGAAGAAGAAGGAGAAGAGCAAGTTCTGGATCGCTTTGTCGAAGGAGGAGATTGAAGAGGACGTGTTCGTGATGACCGGGTCGAGGCCCCCCAGAAGGCCCAAGAAGCGGCCCAAGAATGTTCAGAAACAACTCGATAGTATTTTTCCTGGGTTGTGGCTGCTTGGTGTCACTGCAGATGCTTATAAGTCTGCAGATTCTCCATGTAAGGTAGATTTGTATGGTGACTTGAAGATTGTGATTTTCTCGAAACTGCGTAAATGGAAGTTGCTGCTGCACCTGTGGAGTGGAAGCCTCAATTTTTTGAGGATGGAAGTCAGCTTAGCTGCTTCTTCAAGTATCAAGAAATTGGTACAAAATATTCTGCAGAGTGTAACAGGTTTCACATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

292

Amino Acids

32.91

Weight (kDa)

9.77

Isoelectric Point (pI)

68.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 174 - 223 1.4e-28 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AccBSI CCGCTC 1 cut(s) 241
AccII CGCG 1 cut(s) 177
AclWI GGATC 2 cut(s) 196, 521
AcsI RAATTY 1 cut(s) 295
AcuI CTGAAG 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 326
AfaI GTAC 1 cut(s) 837
AfiI CCNNNNNNNGG 4 cut(s) 211, 567, 579, 591
AflIII ACRYGT 1 cut(s) 546
AgsI TTSAA 5 cut(s) 55, 335, 539, 715, 819
AjiI CACGTC 2 cut(s) 163, 547
AjnI CCWGG 1 cut(s) 631
AluBI AGCT 4 cut(s) 269, 314, 804, 809
AluI AGCT 4 cut(s) 269, 314, 804, 809
Alw21I GWGCWC 2 cut(s) 212, 316
AlwI GGATC 2 cut(s) 196, 521
AoxI GGCC 7 cut(s) 123, 302, 346, 395, 569, 581, 593
ApoI RAATTY 1 cut(s) 295
AspLEI GCGC 3 cut(s) 13, 179, 382
AspS9I GGNCC 7 cut(s) 168, 303, 347, 395, 570, 582, 594
AsuC2I CCSGG 2 cut(s) 374, 562
AsuHPI GGTGA 1 cut(s) 719
AvaII GGWCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 316
Bbv12I GWGCWC 2 cut(s) 212, 316
BbvCI CCTCAGC 1 cut(s) 270
BccI CCATC 1 cut(s) 787
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 1 cut(s) 633
BcnI CCSGG 2 cut(s) 374, 562
BfmI CTRYAG 3 cut(s) 657, 675, 848
BfoI RGCGCY 1 cut(s) 14
BlpI GCTNAGC 1 cut(s) 805
Bme1390I CCNGG 3 cut(s) 374, 562, 633
Bme18I GGWCC 1 cut(s) 168
BmgBI CACGTC 2 cut(s) 163, 547
BmgT120I GGNCC 7 cut(s) 168, 303, 347, 395, 570, 582, 594
BmiI GGNNCC 4 cut(s) 169, 387, 396, 572
BmrFI CCNGG 3 cut(s) 374, 562, 633
BmsI GCATC 2 cut(s) 411, 652
Bpu10I CCTNAGC 1 cut(s) 270
Bpu1102I GCTNAGC 1 cut(s) 805
BpuMI CCSGG 2 cut(s) 374, 562
BsaHI GRCGYC 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 290, 632
BsaWI WCCGGW 2 cut(s) 388, 402
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 4 cut(s) 211, 567, 579, 591
Bse118I RCCGGY 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 633
BseDI CCNNGG 2 cut(s) 290, 632
BseGI GGATG 1 cut(s) 798
BseLI CCNNNNNNNGG 4 cut(s) 211, 567, 579, 591
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 4 cut(s) 103, 251, 275, 545
BsgI GTGCAG 2 cut(s) 14, 743
Bsh1236I CGCG 1 cut(s) 177
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 7 cut(s) 125, 304, 348, 397, 571, 583, 595
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 2 cut(s) 212, 316
BsiSI CCGG 5 cut(s) 122, 373, 389, 403, 561
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 4 cut(s) 211, 567, 579, 591
BsmFI GGGAC 1 cut(s) 76
BsnI GGCC 7 cut(s) 125, 304, 348, 397, 571, 583, 595
Bsp1286I GDGCHC 2 cut(s) 212, 316
Bsp143I GATC 4 cut(s) 181, 201, 250, 513
Bsp1720I GCTNAGC 1 cut(s) 805
Bsp19I CCATGG 1 cut(s) 290
BspANI GGCC 7 cut(s) 125, 304, 348, 397, 571, 583, 595
BspCNI CTCAG 1 cut(s) 262
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 4 cut(s) 169, 387, 396, 572
BspMAI CTGCAG 3 cut(s) 661, 679, 852
BspPI GGATC 2 cut(s) 196, 521
BspQI GCTCTTC 2 cut(s) 304, 494
BsrBI CCGCTC 1 cut(s) 241
BsrFI RCCGGY 1 cut(s) 121
BsrI ACTGG 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 121
BssECI CCNNGG 2 cut(s) 290, 632
BssMI GATC 4 cut(s) 181, 201, 250, 513
BssNI GRCGYC 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 633
Bst6I CTCTTC 4 cut(s) 252, 304, 494, 534
BstACI GRCGYC 1 cut(s) 326
BstDEI CTNAG 3 cut(s) 66, 270, 805
BstDSI CCRYGG 1 cut(s) 290
BstF5I GGATG 1 cut(s) 798
BstFNI CGCG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 14
BstHHI GCGC 3 cut(s) 13, 179, 382
BstKTI GATC 4 cut(s) 184, 204, 253, 516
BstMBI GATC 4 cut(s) 181, 201, 250, 513
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 6 cut(s) 183, 362, 365, 430, 433, 589
BstNI CCWGG 1 cut(s) 633
BstSCI CCNGG 3 cut(s) 372, 560, 631
BstSFI CTRYAG 3 cut(s) 657, 675, 848
BstUI CGCG 1 cut(s) 177
BsuRI GGCC 7 cut(s) 125, 304, 348, 397, 571, 583, 595
BtgI CCRYGG 1 cut(s) 290
BtrI CACGTC 2 cut(s) 163, 547
BtsCI GGATG 1 cut(s) 798
BtsI GCAGTG 2 cut(s) 75, 654
BtsIMutI CAGTG 3 cut(s) 21, 75, 654
CfoI GCGC 3 cut(s) 13, 179, 382
Cfr10I RCCGGY 1 cut(s) 121
Cfr13I GGNCC 7 cut(s) 168, 303, 347, 395, 570, 582, 594
CseI GACGC 2 cut(s) 212, 315
Csp6I GTAC 1 cut(s) 836
CviAII CATG 2 cut(s) 291, 687
CviQI GTAC 1 cut(s) 836
DdeI CTNAG 3 cut(s) 66, 270, 805
DpnI GATC 4 cut(s) 183, 203, 252, 515
DpnII GATC 4 cut(s) 181, 201, 250, 513
Eam1104I CTCTTC 4 cut(s) 252, 304, 494, 534
EarI CTCTTC 4 cut(s) 252, 304, 494, 534
EciI GGCGGA 1 cut(s) 479
Ecl136II GAGCTC 1 cut(s) 314
Eco130I CCWWGG 1 cut(s) 290
Eco24I GRGCYC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 168
Eco53kI GAGCTC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 314
EcoO109I RGGNCCY 2 cut(s) 168, 570
EcoRII CCWGG 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 290
EcoT38I GRGCYC 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 2 cut(s) 294, 690
FaiI YATR 6 cut(s) 156, 292, 669, 688, 705, 874
FaqI GGGAC 1 cut(s) 76
FatI CATG 2 cut(s) 290, 686
FauI CCCGC 3 cut(s) 115, 182, 234
FokI GGATG 1 cut(s) 805
FriOI GRGCYC 1 cut(s) 316
GlaI GCGC 3 cut(s) 12, 178, 381
HaeII RGCGCY 1 cut(s) 14
HaeIII GGCC 7 cut(s) 125, 304, 348, 397, 571, 583, 595
HapII CCGG 5 cut(s) 122, 373, 389, 403, 561
HgaI GACGC 2 cut(s) 212, 315
HhaI GCGC 3 cut(s) 13, 179, 382
Hin1I GRCGYC 1 cut(s) 326
Hin1II CATG 2 cut(s) 294, 690
Hin6I GCGC 3 cut(s) 11, 177, 380
HinP1I GCGC 3 cut(s) 11, 177, 380
HinfI GANTC 3 cut(s) 137, 406, 680
HpaII CCGG 5 cut(s) 122, 373, 389, 403, 561
HphI GGTGA 1 cut(s) 719
Hpy188I TCNGA 7 cut(s) 118, 130, 142, 214, 285, 411, 609
Hpy188III TCNNGA 6 cut(s) 52, 254, 511, 553, 730, 826
Hpy99I CGWCG 4 cut(s) 167, 228, 325, 331
HpyAV CCTTC 3 cut(s) 487, 520, 573
HpyCH4IV ACGT 2 cut(s) 162, 546
HpyCH4V TGCA 7 cut(s) 31, 368, 424, 659, 677, 760, 850
HpyF10VI GCNNNNNNNGC 6 cut(s) 183, 362, 365, 430, 433, 589
HpyF3I CTNAG 3 cut(s) 66, 270, 805
HpySE526I ACGT 2 cut(s) 162, 546
Hsp92I GRCGYC 1 cut(s) 326
Hsp92II CATG 2 cut(s) 294, 690
HspAI GCGC 3 cut(s) 11, 177, 380
Kzo9I GATC 4 cut(s) 181, 201, 250, 513
LguI GCTCTTC 2 cut(s) 304, 494
LmnI GCTCC 2 cut(s) 231, 238
LweI GCATC 2 cut(s) 411, 652
MaeII ACGT 2 cut(s) 162, 546
MaeIII GTNAC 3 cut(s) 652, 707, 856
MalI GATC 4 cut(s) 183, 203, 252, 515
MbiI CCGCTC 1 cut(s) 241
MboI GATC 4 cut(s) 181, 201, 250, 513
MboII GAAGA 9 cut(s) 269, 321, 496, 499, 502, 511, 551, 727, 807
MhlI GDGCHC 2 cut(s) 212, 316
MluCI AATT 3 cut(s) 295, 781, 830
MlyI GAGTC 2 cut(s) 131, 415
MmeI TCCRAC 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 5 cut(s) 122, 373, 389, 403, 561
MspR9I CCNGG 3 cut(s) 374, 562, 633
MvaI CCWGG 1 cut(s) 633
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 6 cut(s) 183, 362, 365, 430, 433, 589
NciI CCSGG 2 cut(s) 374, 562
NcoI CCATGG 1 cut(s) 290
NdeII GATC 4 cut(s) 181, 201, 250, 513
NlaIII CATG 2 cut(s) 294, 690
NlaIV GGNNCC 4 cut(s) 169, 387, 396, 572
NmuCI GTSAC 2 cut(s) 652, 707
PciSI GCTCTTC 2 cut(s) 304, 494
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PfeI GAWTC 1 cut(s) 680
PflFI GACNNNGTC 2 cut(s) 325, 562
Ple19I CGATCG 1 cut(s) 184
PleI GAGTC 2 cut(s) 131, 414
PpsI GAGTC 2 cut(s) 131, 414
PpuMI RGGWCCY 1 cut(s) 168
PsiI TTATAA 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 316
Psp5II RGGWCCY 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 631
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 4 cut(s) 169, 387, 396, 572
PspPI GGNCC 7 cut(s) 168, 303, 347, 395, 570, 582, 594
PspPPI RGGWCCY 1 cut(s) 168
PstI CTGCAG 3 cut(s) 661, 679, 852
PsyI GACNNNGTC 2 cut(s) 325, 562
PvuI CGATCG 1 cut(s) 184
RsaI GTAC 1 cut(s) 837
RsaNI GTAC 1 cut(s) 836
SacI GAGCTC 1 cut(s) 316
SapI GCTCTTC 2 cut(s) 304, 494
Sau3AI GATC 4 cut(s) 181, 201, 250, 513
Sau96I GGNCC 7 cut(s) 168, 303, 347, 395, 570, 582, 594
SchI GAGTC 2 cut(s) 131, 415
ScrFI CCNGG 3 cut(s) 374, 562, 633
SduI GDGCHC 2 cut(s) 212, 316
SfaNI GCATC 2 cut(s) 411, 652
SfcI CTRYAG 3 cut(s) 657, 675, 848
SinI GGWCC 1 cut(s) 168
Sse9I AATT 3 cut(s) 295, 781, 830
SspI AATATT 1 cut(s) 844
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 3 cut(s) 372, 560, 631
StyI CCWWGG 1 cut(s) 290
TaiI ACGT 2 cut(s) 165, 549
TaqI TCGA 5 cut(s) 443, 524, 566, 618, 731
TasI AATT 3 cut(s) 295, 781, 830
TauI GCSGC 3 cut(s) 28, 362, 595
TfiI GAWTC 1 cut(s) 680
TscAI CASTG 3 cut(s) 21, 82, 661
TseFI GTSAC 2 cut(s) 652, 707
Tsp45I GTSAC 2 cut(s) 652, 707
TspRI CASTG 3 cut(s) 21, 82, 661
Tth111I GACNNNGTC 2 cut(s) 325, 562
VpaK11BI GGWCC 1 cut(s) 168
XapI RAATTY 1 cut(s) 295
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.