Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010364

Physical Location & Seq

Genomic Coordinates Forward (+)

9963083 .. 9972748

Gene Structure 9666 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1072900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGCATTTCTGAGGACAGTGGCCACGACTAATGCCATAGCTGCTGCCTCTGCATTTCCTGCGGCCGTTGCCCTTTCTTCCTTCTCTTTTTCTTCCTCCTCTGTATCTTTTCGATTCCCAAAACCCCAAAACCTCTCCTTTCTCTCCTCCGCGCCTTCTCTGCTGAACCCAAATCCTCCTTTCCTCGCTACAAGCTTCGCCCGCTCCCCCACGGCTATCCAGATGGACGCGCCCACATCGGAGCAACAGCAATCCTTTCAGAGTGATGGCGCTTTGCCGGAGCTACTGACAGAGTACATGGTGGATATGAAATGTGATGGATGTGTCAAGTCTGTAAAGAGCAAGTTAGAAACTGTGGATGGAGTAAAAAGTGTAGAGGTAGACCTCAACAGTCAAGTCGTCAGGGTTCTGGGTTCAACGCCTTTGAAGACCATGACTGAAGCTTTGGAGCAGACTGGTCGAAAAGCAAGATTAATTGGGCAGGGGATTCCTGAAGATTTCTTGGTTTCTGCGGCTGTTTCTGAGTTCAAGGGTCCAAATATTTTTGGTGTGGTTCGTTTTGCTCAGGTGAATATGGAATTGGCTAGGATTGAGGCCAACTTTAGTGGATTAACACCTGGAAAACATGGTTGGTCGATCAACGAATTTGGTGATCTAACGGAAGGTGCTGCAAGCACTGGAAAAGTATTTAAGCCATCAAATGAAGCAAAGGAGCCGCTTGGTGACCTGGGAACACTGGTAAGTGATGAGAGTGGTAATGCCTTCTTCTCTGGGGTCAAAGAGAAGCTGAGAGTTTCGGATCTTATCGGGCGGTCAATAGCGATATATGAAACTGCCGATAAATCGGACACTGGTATCGCGGCTGCTGTGATTGCTAGAAGTGCTGGAGTCGGGGAGAACTACAAAAAACTTTGCACTTGCGATGGAACCACCATATGGGAATCAACTGGCAACGACTTCGTCGCCAGCGAGGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006873 GO:0006875 GO:0006878 GO:0008047 GO:0008150 GO:0008270 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016530 GO:0016531 GO:0016532 GO:0019725 GO:0030003 GO:0030234 GO:0042592 GO:0043085 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050790 GO:0050801 GO:0055065 GO:0055070 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0065009 GO:0098771 GO:0098772 GO:0140104

KEGG Pathways

Metabolic & Signaling

ko05014 map05014

Pfam Domains

Protein Families

HMA Sod_Cu

Protein Analysis

326

Amino Acids

34.38

Weight (kDa)

5.01

Isoelectric Point (pI)

32.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	100 - 155	1.8e-12	Heavy-metal-associated domain
Sod_Cu	PF00080	183 - 287	6.4e-18	Copper/zinc superoxide dismutase (SODC)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010364)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12520 AT1G12520 AT1G12520
fragaria_vesca	FvH4_5g01090
malus_domestica	MD00G1069800.v1.1 MD04G1072900.v1.1
prunus_persica	Prupe.5G084900_v2.0.a1 Prupe.5G084900_v2.0.a1
pyrus_communis	pycom04g06600 pycom06g06690
rosa_chinensis	RchiOBHm_Chr7g0200231
rosa_laevigata	RLG00000003742
rosa_multiflora	Rmu_sc0019284.1_g000001 Rmu_sc0025955.1_g000002
rosa_roxburghii	Rroxscaffold_3G00256210
rosa_rugosa	Rorug07G0058600
rosa_samantha	Rh7AG184100 Rh7BG186800 Rh7CG194500 Rh7DG188200
rosa_wichuraiana	Rw7G016110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	936
AccBSI	CCGCTC	1 cut(s)	204
AccI	GTMKAC	1 cut(s)	381
AccII	CGCG	3 cut(s)	152, 230, 860
AciI	CCGC	7 cut(s)	62, 150, 202, 512, 716, 811, 860
AclWI	GGATC	1 cut(s)	807
AcoI	YGGCCR	2 cut(s)	21, 63
AcsI	RAATTY	1 cut(s)	644
AcuI	CTGAAG	2 cut(s)	459, 513
AfaI	GTAC	1 cut(s)	296
AfiI	CCNNNNNNNGG	1 cut(s)	936
AgsI	TTSAA	3 cut(s)	417, 427, 529
AjnI	CCWGG	2 cut(s)	616, 726
AjuI	GAANNNNNNNTTGG	4 cut(s)	563, 595, 613, 645
AluBI	AGCT	5 cut(s)	41, 195, 283, 443, 787
AluI	AGCT	5 cut(s)	41, 195, 283, 443, 787
AlwI	GGATC	1 cut(s)	807
AoxI	GGCC	3 cut(s)	21, 63, 594
ApeKI	GCWGC	4 cut(s)	41, 44, 668, 863
ApoI	RAATTY	1 cut(s)	644
ArsI	GACNNNNNNTTYG	2 cut(s)	427, 459
AseI	ATTAAT	1 cut(s)	473
AspLEI	GCGC	3 cut(s)	154, 232, 272
AspS9I	GGNCC	1 cut(s)	533
AsuHPI	GGTGA	3 cut(s)	580, 662, 734
AvaII	GGWCC	1 cut(s)	533
BaeI	ACNNNNGTAYC	2 cut(s)	838, 871
BalI	TGGCCA	1 cut(s)	23
BbsI	GAAGAC	1 cut(s)	434
BbvI	GCAGC	4 cut(s)	28, 31, 655, 850
BccI	CCATC	6 cut(s)	217, 260, 311, 353, 703, 917
BceAI	ACGGC	2 cut(s)	50, 228
BcgI	CGANNNNNNTGC	4 cut(s)	440, 474, 940, 974
BciT130I	CCWGG	2 cut(s)	618, 728
BfaI	CTAG	2 cut(s)	585, 876
BfoI	RGCGCY	1 cut(s)	273
BisI	GCNGC	8 cut(s)	42, 45, 63, 513, 669, 716, 861, 864
BlsI	GCNGC	8 cut(s)	43, 46, 64, 514, 670, 717, 862, 865
Bme1390I	CCNGG	2 cut(s)	618, 728
Bme18I	GGWCC	1 cut(s)	533
BmgT120I	GGNCC	1 cut(s)	533
BmiI	GGNNCC	3 cut(s)	534, 714, 928
BmrFI	CCNGG	2 cut(s)	618, 728
BpiI	GAAGAC	1 cut(s)	434
BpmI	CTGGAG	1 cut(s)	906
Bpu10I	CCTNAGC	1 cut(s)	564
BsaJI	CCNNGG	2 cut(s)	210, 727
Bsc4I	CCNNNNNNNGG	1 cut(s)	936
Bse1I	ACTGG	5 cut(s)	460, 682, 741, 856, 952
BseBI	CCWGG	2 cut(s)	618, 728
BseDI	CCNNGG	2 cut(s)	210, 727
BseGI	GGATG	2 cut(s)	326, 364
BseLI	CCNNNNNNNGG	1 cut(s)	936
BseMII	CTCAG	3 cut(s)	513, 578, 779
BseNI	ACTGG	5 cut(s)	460, 682, 741, 856, 952
BseRI	GAGGAG	2 cut(s)	88, 136
BseX3I	CGGCCG	1 cut(s)	63
BseXI	GCAGC	4 cut(s)	28, 31, 655, 850
Bsh1236I	CGCG	3 cut(s)	152, 230, 860
Bsh1285I	CGRYCG	1 cut(s)	66
BshFI	GGCC	3 cut(s)	23, 65, 596
BsiEI	CGRYCG	1 cut(s)	66
BsiSI	CCGG	1 cut(s)	278
BslI	CCNNNNNNNGG	1 cut(s)	936
BsnI	GGCC	3 cut(s)	23, 65, 596
Bsp143I	GATC	3 cut(s)	636, 652, 799
BspACI	CCGC	7 cut(s)	62, 150, 202, 512, 716, 811, 860
BspANI	GGCC	3 cut(s)	23, 65, 596
BspCNI	CTCAG	3 cut(s)	514, 577, 780
BspFNI	CGCG	3 cut(s)	152, 230, 860
BspLI	GGNNCC	3 cut(s)	534, 714, 928
BspPI	GGATC	1 cut(s)	807
BsrBI	CCGCTC	1 cut(s)	204
BsrI	ACTGG	5 cut(s)	460, 682, 741, 856, 952
BssECI	CCNNGG	2 cut(s)	210, 727
BssMI	GATC	3 cut(s)	636, 652, 799
Bst2UI	CCWGG	2 cut(s)	618, 728
Bst4CI	ACNGT	3 cut(s)	19, 355, 392
BstAPI	GCANNNNNTGC	1 cut(s)	59
BstC8I	GCNNGC	3 cut(s)	202, 673, 967
BstDEI	CTNAG	4 cut(s)	11, 522, 564, 788
BstDSI	CCRYGG	1 cut(s)	210
BstEII	GGTNACC	1 cut(s)	722
BstF5I	GGATG	2 cut(s)	326, 364
BstFNI	CGCG	3 cut(s)	152, 230, 860
BstH2I	RGCGCY	1 cut(s)	273
BstHHI	GCGC	3 cut(s)	154, 232, 272
BstKTI	GATC	3 cut(s)	639, 655, 802
BstMBI	GATC	3 cut(s)	636, 652, 799
BstMCI	CGRYCG	1 cut(s)	66
BstMWI	GCNNNNNNNGC	8 cut(s)	41, 50, 59, 68, 160, 201, 872, 881
BstNI	CCWGG	2 cut(s)	618, 728
BstPI	GGTNACC	1 cut(s)	722
BstSCI	CCNGG	2 cut(s)	616, 726
BstUI	CGCG	3 cut(s)	152, 230, 860
BstV1I	GCAGC	4 cut(s)	28, 31, 655, 850
BstV2I	GAAGAC	1 cut(s)	434
BstX2I	RGATCY	1 cut(s)	799
BstYI	RGATCY	1 cut(s)	799
BstZI	CGGCCG	1 cut(s)	63
BsuRI	GGCC	3 cut(s)	23, 65, 596
BtgI	CCRYGG	1 cut(s)	210
BtgZI	GCGATG	1 cut(s)	936
BtsCI	GGATG	2 cut(s)	326, 364
BtsIMutI	CAGTG	4 cut(s)	24, 675, 734, 849
Cac8I	GCNNGC	3 cut(s)	202, 673, 967
CfoI	GCGC	3 cut(s)	154, 232, 272
Cfr13I	GGNCC	1 cut(s)	533
CseI	GACGC	1 cut(s)	236
Csp6I	GTAC	1 cut(s)	295
CspCI	CAANNNNNGTGG	2 cut(s)	586, 621
CviAII	CATG	3 cut(s)	298, 433, 626
CviQI	GTAC	1 cut(s)	295
DdeI	CTNAG	4 cut(s)	11, 522, 564, 788
DpnI	GATC	3 cut(s)	638, 654, 801
DpnII	GATC	3 cut(s)	636, 652, 799
EaeI	YGGCCR	2 cut(s)	21, 63
EagI	CGGCCG	1 cut(s)	63
EclXI	CGGCCG	1 cut(s)	63
Eco47I	GGWCC	1 cut(s)	533
Eco52I	CGGCCG	1 cut(s)	63
Eco57I	CTGAAG	2 cut(s)	459, 513
Eco91I	GGTNACC	1 cut(s)	722
EcoO65I	GGTNACC	1 cut(s)	722
EcoRII	CCWGG	2 cut(s)	616, 726
FaeI	CATG	3 cut(s)	301, 436, 629
FatI	CATG	3 cut(s)	297, 432, 625
FauI	CCCGC	1 cut(s)	209
FauNDI	CATATG	1 cut(s)	935
FblI	GTMKAC	1 cut(s)	381
Fnu4HI	GCNGC	8 cut(s)	42, 45, 63, 513, 669, 716, 861, 864
FokI	GGATG	2 cut(s)	333, 371
Fsp4HI	GCNGC	8 cut(s)	42, 45, 63, 513, 669, 716, 861, 864
FspBI	CTAG	2 cut(s)	585, 876
GlaI	GCGC	3 cut(s)	153, 231, 271
GluI	GCNGC	8 cut(s)	42, 45, 63, 513, 669, 716, 861, 864
GsuI	CTGGAG	1 cut(s)	906
HaeII	RGCGCY	1 cut(s)	273
HaeIII	GGCC	3 cut(s)	23, 65, 596
HapII	CCGG	1 cut(s)	278
HgaI	GACGC	1 cut(s)	236
HhaI	GCGC	3 cut(s)	154, 232, 272
Hin1II	CATG	3 cut(s)	301, 436, 629
Hin6I	GCGC	3 cut(s)	152, 230, 270
HinP1I	GCGC	3 cut(s)	152, 230, 270
HindIII	AAGCTT	2 cut(s)	193, 441
HinfI	GANTC	4 cut(s)	114, 487, 888, 941
HpaII	CCGG	1 cut(s)	278
HphI	GGTGA	3 cut(s)	580, 662, 734
Hpy166II	GTNNAC	1 cut(s)	382
Hpy188I	TCNGA	7 cut(s)	12, 241, 261, 523, 799, 847, 977
Hpy188III	TCNNGA	2 cut(s)	220, 491
Hpy8I	GTNNAC	1 cut(s)	382
Hpy99I	CGWCG	1 cut(s)	965
HpyAV	CCTTC	4 cut(s)	91, 165, 656, 772
HpyCH4III	ACNGT	3 cut(s)	19, 355, 392
HpyCH4V	TGCA	3 cut(s)	53, 671, 915
HpyF10VI	GCNNNNNNNGC	8 cut(s)	41, 50, 59, 68, 160, 201, 872, 881
HpyF3I	CTNAG	4 cut(s)	11, 522, 564, 788
Hsp92II	CATG	3 cut(s)	301, 436, 629
HspAI	GCGC	3 cut(s)	152, 230, 270
Kzo9I	GATC	3 cut(s)	636, 652, 799
LmnI	GCTCC	5 cut(s)	209, 241, 280, 448, 712
Lsp1109I	GCAGC	4 cut(s)	28, 31, 655, 850
MaeI	CTAG	2 cut(s)	585, 876
MaeIII	GTNAC	1 cut(s)	722
MalI	GATC	3 cut(s)	638, 654, 801
MbiI	CCGCTC	1 cut(s)	204
MboI	GATC	3 cut(s)	636, 652, 799
MboII	GAAGA	5 cut(s)	69, 84, 439, 506, 757
MflI	RGATCY	1 cut(s)	799
MlsI	TGGCCA	1 cut(s)	23
MluCI	AATT	3 cut(s)	474, 578, 644
MluNI	TGGCCA	1 cut(s)	23
MlyI	GAGTC	1 cut(s)	897
Mox20I	TGGCCA	1 cut(s)	23
MscI	TGGCCA	1 cut(s)	23
MseI	TTAA	3 cut(s)	473, 611, 690
Msp20I	TGGCCA	1 cut(s)	23
MspI	CCGG	1 cut(s)	278
MspR9I	CCNGG	2 cut(s)	618, 728
MvaI	CCWGG	2 cut(s)	618, 728
MvnI	CGCG	3 cut(s)	152, 230, 860
MwoI	GCNNNNNNNGC	8 cut(s)	41, 50, 59, 68, 160, 201, 872, 881
NdeI	CATATG	1 cut(s)	935
NdeII	GATC	3 cut(s)	636, 652, 799
NlaIII	CATG	3 cut(s)	301, 436, 629
NlaIV	GGNNCC	3 cut(s)	534, 714, 928
NmuCI	GTSAC	1 cut(s)	722
PcsI	WCGNNNNNNNCGW	1 cut(s)	966
PfeI	GAWTC	3 cut(s)	114, 487, 941
PflFI	GACNNNGTC	1 cut(s)	959
PflMI	CCANNNNNTGG	1 cut(s)	936
PkrI	GCNGC	8 cut(s)	43, 46, 64, 514, 670, 717, 862, 865
PleI	GAGTC	1 cut(s)	896
PpsI	GAGTC	1 cut(s)	896
PshBI	ATTAAT	1 cut(s)	473
Psp6I	CCWGG	2 cut(s)	616, 726
PspEI	GGTNACC	1 cut(s)	722
PspGI	CCWGG	2 cut(s)	616, 726
PspN4I	GGNNCC	3 cut(s)	534, 714, 928
PspPI	GGNCC	1 cut(s)	533
PsuI	RGATCY	1 cut(s)	799
PsyI	GACNNNGTC	1 cut(s)	959
RsaI	GTAC	1 cut(s)	296
RsaNI	GTAC	1 cut(s)	295
SaqAI	TTAA	3 cut(s)	473, 611, 690
SatI	GCNGC	8 cut(s)	42, 45, 63, 513, 669, 716, 861, 864
Sau3AI	GATC	3 cut(s)	636, 652, 799
Sau96I	GGNCC	1 cut(s)	533
SchI	GAGTC	1 cut(s)	897
ScrFI	CCNGG	2 cut(s)	618, 728
SinI	GGWCC	1 cut(s)	533
Sse9I	AATT	3 cut(s)	474, 578, 644
SsiI	CCGC	7 cut(s)	62, 150, 202, 512, 716, 811, 860
SspI	AATATT	1 cut(s)	541
SspMI	CTAG	2 cut(s)	585, 876
StyD4I	CCNGG	2 cut(s)	616, 726
TaaI	ACNGT	3 cut(s)	19, 355, 392
TaqI	TCGA	3 cut(s)	112, 460, 635
TasI	AATT	3 cut(s)	474, 578, 644
TatI	WGTACW	1 cut(s)	294
TauI	GCSGC	4 cut(s)	65, 515, 718, 863
TfiI	GAWTC	3 cut(s)	114, 487, 941
Tru1I	TTAA	3 cut(s)	473, 611, 690
Tru9I	TTAA	3 cut(s)	473, 611, 690
TscAI	CASTG	4 cut(s)	24, 682, 741, 856
TseFI	GTSAC	1 cut(s)	722
TseI	GCWGC	4 cut(s)	41, 44, 668, 863
Tsp45I	GTSAC	1 cut(s)	722
TspDTI	ATGAA	3 cut(s)	323, 717, 843
TspGWI	ACGGA	1 cut(s)	674
TspRI	CASTG	4 cut(s)	24, 682, 741, 856
Tth111I	GACNNNGTC	1 cut(s)	959
Van91I	CCANNNNNTGG	1 cut(s)	936
VpaK11BI	GGWCC	1 cut(s)	533
VspI	ATTAAT	1 cut(s)	473
XapI	RAATTY	1 cut(s)	644
XmiI	GTMKAC	1 cut(s)	381
XspI	CTAG	2 cut(s)	585, 876

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.