MD04G1078200.v1.1

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004031
Physical Location & Seq
Forward (+)
10724967 .. 10728580
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1078200.v1.1.491

Sequence Viewer

Length: 894 bp
ATGCTGCCCTACCAGACCATCGAGGAGGCCTCGGCGGCTTTGGGCCGAAACCTCACCTTCGCCGAGACACTGTGGTTCAACTACTCTGCGTCCAAGTCCGATTACGTCCTCTACTGCCACAACCTTCTCTTCCTCTTCGCCATCTTCTCCCTCGTCCCTCTCCCTCTGGTTTTCATGGAGGTTCTCAGATGGGGCGGCCTCGACCGCTACAAGATACAGCCCAAAGTCCGATTGCCCTTCTCTGACATGTTGAAATGCTACAAGGACGTTATGTGGATGTTCTTTTTCATCGTCGGCCCTCTCCAGCTCGTCTCTTACCCTTCAATCCAGATGATCGGGATTCGAACAGGATTGCCATTGCCGTCTGGATGGGAGATGCTTTCCCAGTTGTTAGTTTATTTCTTGGTAGAAGATTATACCAACTACTGGATCCACAGATTTTTGCATAACAAATGGGGGTACGAGAAAATCCATCGAGTTCACCACGAGTACACTGCTCCAATTGGATTTGCAGCGCCATATGCGCATTGGGCTGAGATTTTGATCCTCGGCATCCCATCTTTTCTTGGTCCGGCCATGGTTCCTGGCCACATGATCACATTCTGGTCGTGGATAGCATTGCGGCAGATTGAGGCCATAGAAACACACAGCGGTTATGACTTCCCCTGGACTCCCACAAAGCATATCCCATTTTATGGTGGTGCTGAGTATCACGATTACCATCATTATGTTGGAGGACAAAGCCAGAGTAATTTTGCTTCAGTGTTCACCTATTGTGATTACATTTATGGAACAGACAAGGGATATCGGTACCAGAAGAAGATCCTTAAGAAGTTGAACGAGGAACCTGCTGGAGTCCAAAATGGAGGCTCCATAGATTTTAAAACTGATTAG

Protein Analysis

298

Amino Acids

34.58

Weight (kDa)

6.66

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 131 - 265 5.4e-27 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 525
Acc36I ACCTGC 1 cut(s) 856
Acc65I GGTACC 1 cut(s) 810
AccB1I GGYRCC 1 cut(s) 810
AccB7I CCANNNNNTGG 2 cut(s) 426, 695
AciI CCGC 5 cut(s) 35, 195, 205, 622, 651
AclWI GGATC 4 cut(s) 424, 437, 538, 817
AcoI YGGCCR 2 cut(s) 573, 586
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 3 cut(s) 461, 491, 812
AfiI CCNNNNNNNGG 2 cut(s) 426, 695
AflII CTTAAG 1 cut(s) 827
AflIII ACRYGT 1 cut(s) 246
AgsI TTSAA 4 cut(s) 79, 253, 324, 838
AjnI CCWGG 2 cut(s) 583, 665
AluBI AGCT 1 cut(s) 307
AluI AGCT 1 cut(s) 307
Alw26I GTCTC 2 cut(s) 59, 316
AlwI GGATC 4 cut(s) 424, 437, 538, 817
AoxI GGCC 7 cut(s) 27, 43, 196, 295, 573, 586, 633
ApeKI GCWGC 2 cut(s) 4, 512
Asp718I GGTACC 1 cut(s) 810
AspLEI GCGC 2 cut(s) 517, 526
AspS9I GGNCC 3 cut(s) 43, 296, 569
AsuHPI GGTGA 3 cut(s) 46, 473, 760
AsuII TTCGAA 1 cut(s) 343
AvaII GGWCC 1 cut(s) 569
BalI TGGCCA 1 cut(s) 588
BamHI GGATCC 1 cut(s) 429
BanI GGYRCC 1 cut(s) 810
BarI GAAGNNNNNNTAC 2 cut(s) 742, 774
BauI CACGAG 1 cut(s) 485
BbvI GCAGC 1 cut(s) 524
BccI CCATC 7 cut(s) 26, 149, 183, 363, 480, 565, 729
BceAI ACGGC 1 cut(s) 346
BcgI CGANNNNNNTGC 4 cut(s) 476, 510, 830, 864
BciT130I CCWGG 2 cut(s) 585, 667
BclI TGATCA 1 cut(s) 594
BcoDI GTCTC 2 cut(s) 59, 316
BfoI RGCGCY 1 cut(s) 518
BfrI CTTAAG 1 cut(s) 827
BfuAI ACCTGC 1 cut(s) 856
BglI GCCNNNNNGGC 1 cut(s) 35
BisI GCNGC 5 cut(s) 5, 36, 196, 513, 623
BlsI GCNGC 5 cut(s) 6, 37, 197, 514, 624
Bme1390I CCNGG 2 cut(s) 585, 667
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 3 cut(s) 43, 296, 569
BmiI GGNNCC 5 cut(s) 431, 582, 812, 846, 871
BmrFI CCNGG 2 cut(s) 585, 667
BmrI ACTGGG 1 cut(s) 379
BmsI GCATC 2 cut(s) 366, 561
BmuI ACTGGG 1 cut(s) 379
BplI GAGNNNNNCTC 2 cut(s) 14, 46
BpmI CTGGAG 2 cut(s) 287, 873
Bpu14I TTCGAA 1 cut(s) 343
BsaBI GATNNNNATC 2 cut(s) 542, 720
BsaJI CCNNGG 4 cut(s) 30, 547, 576, 665
Bsc4I CCNNNNNNNGG 2 cut(s) 426, 695
Bse1I ACTGG 2 cut(s) 385, 431
Bse3DI GCAATG 2 cut(s) 356, 617
Bse8I GATNNNNATC 2 cut(s) 542, 720
BseBI CCWGG 2 cut(s) 585, 667
BseDI CCNNGG 4 cut(s) 30, 547, 576, 665
BseGI GGATG 3 cut(s) 282, 374, 552
BseJI GATNNNNATC 2 cut(s) 542, 720
BseLI CCNNNNNNNGG 2 cut(s) 426, 695
BseMI GCAATG 2 cut(s) 356, 617
BseMII CTCAG 3 cut(s) 199, 525, 696
BseNI ACTGG 2 cut(s) 385, 431
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 1 cut(s) 524
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 7 cut(s) 29, 45, 198, 297, 575, 588, 635
BshNI GGYRCC 1 cut(s) 810
BsiEI CGRYCG 1 cut(s) 205
BsiSI CCGG 1 cut(s) 572
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 2 cut(s) 426, 695
BsmAI GTCTC 2 cut(s) 59, 316
BsmBI CGTCTC 1 cut(s) 316
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 7 cut(s) 29, 45, 198, 297, 575, 588, 635
Bsp119I TTCGAA 1 cut(s) 343
Bsp143I GATC 5 cut(s) 333, 429, 543, 594, 822
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 5 cut(s) 35, 195, 205, 622, 651
BspANI GGCC 7 cut(s) 29, 45, 198, 297, 575, 588, 635
BspCNI CTCAG 3 cut(s) 198, 526, 697
BspLI GGNNCC 5 cut(s) 431, 582, 812, 846, 871
BspMI ACCTGC 1 cut(s) 856
BspPI GGATC 4 cut(s) 424, 437, 538, 817
BspT104I TTCGAA 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 810
BspTI CTTAAG 1 cut(s) 827
BsrDI GCAATG 2 cut(s) 356, 617
BsrI ACTGG 2 cut(s) 385, 431
BssECI CCNNGG 4 cut(s) 30, 547, 576, 665
BssMI GATC 5 cut(s) 333, 429, 543, 594, 822
BssSI CACGAG 1 cut(s) 485
BssT1I CCWWGG 1 cut(s) 576
Bst2BI CACGAG 1 cut(s) 485
Bst2UI CCWGG 2 cut(s) 585, 667
Bst4CI ACNGT 1 cut(s) 72
Bst6I CTCTTC 2 cut(s) 134, 140
BstAFI CTTAAG 1 cut(s) 827
BstBI TTCGAA 1 cut(s) 343
BstDEI CTNAG 3 cut(s) 185, 534, 705
BstDSI CCRYGG 1 cut(s) 576
BstF5I GGATG 3 cut(s) 282, 374, 552
BstH2I RGCGCY 1 cut(s) 518
BstHHI GCGC 2 cut(s) 517, 526
BstKTI GATC 5 cut(s) 336, 432, 546, 597, 825
BstMAI GTCTC 2 cut(s) 59, 316
BstMBI GATC 5 cut(s) 333, 429, 543, 594, 822
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 5 cut(s) 35, 204, 521, 523, 530
BstNI CCWGG 2 cut(s) 585, 667
BstNSI RCATGY 1 cut(s) 250
BstSCI CCNGG 2 cut(s) 583, 665
BstV1I GCAGC 1 cut(s) 524
BstX2I RGATCY 2 cut(s) 429, 822
BstYI RGATCY 2 cut(s) 429, 822
BsuRI GGCC 7 cut(s) 29, 45, 198, 297, 575, 588, 635
BtgI CCRYGG 1 cut(s) 576
BtsCI GGATG 3 cut(s) 282, 374, 552
BtsI GCAGTG 1 cut(s) 492
BtsIMutI CAGTG 3 cut(s) 68, 492, 768
BveI ACCTGC 1 cut(s) 856
CfoI GCGC 2 cut(s) 517, 526
Cfr13I GGNCC 3 cut(s) 43, 296, 569
CseI GACGC 1 cut(s) 78
Csp6I GTAC 3 cut(s) 460, 490, 811
CviAII CATG 4 cut(s) 175, 247, 577, 592
CviQI GTAC 3 cut(s) 460, 490, 811
DdeI CTNAG 3 cut(s) 185, 534, 705
DpnI GATC 5 cut(s) 335, 431, 545, 596, 824
DpnII GATC 5 cut(s) 333, 429, 543, 594, 822
DraI TTTAAA 1 cut(s) 883
EaeI YGGCCR 2 cut(s) 573, 586
Eam1104I CTCTTC 2 cut(s) 134, 140
EarI CTCTTC 2 cut(s) 134, 140
Eco130I CCWWGG 1 cut(s) 576
Eco147I AGGCCT 1 cut(s) 29
Eco32I GATATC 1 cut(s) 806
Eco47I GGWCC 1 cut(s) 569
Eco57I CTGAAG 1 cut(s) 744
EcoRII CCWGG 2 cut(s) 583, 665
EcoRV GATATC 1 cut(s) 806
EcoT14I CCWWGG 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 576
Esp3I CGTCTC 1 cut(s) 316
FaeI CATG 4 cut(s) 178, 250, 580, 595
FaqI GGGAC 1 cut(s) 140
FatI CATG 4 cut(s) 174, 246, 576, 591
FauNDI CATATG 1 cut(s) 520
FbaI TGATCA 1 cut(s) 594
Fnu4HI GCNGC 5 cut(s) 5, 36, 196, 513, 623
FokI GGATG 3 cut(s) 289, 381, 539
Fsp4HI GCNGC 5 cut(s) 5, 36, 196, 513, 623
FspAI RTGCGCAY 1 cut(s) 525
FspI TGCGCA 1 cut(s) 525
GlaI GCGC 2 cut(s) 516, 525
GluI GCNGC 5 cut(s) 5, 36, 196, 513, 623
GsuI CTGGAG 2 cut(s) 287, 873
HaeII RGCGCY 1 cut(s) 518
HaeIII GGCC 7 cut(s) 29, 45, 198, 297, 575, 588, 635
HapII CCGG 1 cut(s) 572
HgaI GACGC 1 cut(s) 78
HhaI GCGC 2 cut(s) 517, 526
Hin1II CATG 4 cut(s) 178, 250, 580, 595
Hin6I GCGC 2 cut(s) 515, 524
HinP1I GCGC 2 cut(s) 515, 524
HinfI GANTC 3 cut(s) 340, 670, 855
HpaII CCGG 1 cut(s) 572
HphI GGTGA 3 cut(s) 46, 473, 760
Hpy166II GTNNAC 3 cut(s) 481, 492, 768
Hpy188I TCNGA 4 cut(s) 100, 188, 230, 244
Hpy188III TCNNGA 4 cut(s) 328, 337, 366, 713
Hpy8I GTNNAC 3 cut(s) 481, 492, 768
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 4 cut(s) 67, 134, 247, 330
HpyCH4III ACNGT 1 cut(s) 72
HpyCH4IV ACGT 2 cut(s) 105, 267
HpyCH4V TGCA 2 cut(s) 445, 512
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 204, 521, 523, 530
HpyF3I CTNAG 3 cut(s) 185, 534, 705
HpySE526I ACGT 2 cut(s) 105, 267
Hsp92II CATG 4 cut(s) 178, 250, 580, 595
HspAI GCGC 2 cut(s) 515, 524
KpnI GGTACC 1 cut(s) 814
Ksp22I TGATCA 1 cut(s) 594
Kzo9I GATC 5 cut(s) 333, 429, 543, 594, 822
LmnI GCTCC 2 cut(s) 502, 875
Lsp1109I GCAGC 1 cut(s) 524
LweI GCATC 2 cut(s) 366, 561
MaeII ACGT 2 cut(s) 105, 267
MalI GATC 5 cut(s) 335, 431, 545, 596, 824
MboI GATC 5 cut(s) 333, 429, 543, 594, 822
MboII GAAGA 6 cut(s) 121, 127, 136, 422, 829, 832
MfeI CAATTG 1 cut(s) 501
MflI RGATCY 2 cut(s) 429, 822
MlsI TGGCCA 1 cut(s) 588
MluCI AATT 2 cut(s) 501, 751
MluNI TGGCCA 1 cut(s) 588
MlyI GAGTC 2 cut(s) 664, 864
MmeI TCCRAC 1 cut(s) 712
Mox20I TGGCCA 1 cut(s) 588
MscI TGGCCA 1 cut(s) 588
MseI TTAA 2 cut(s) 828, 882
MslI CAYNNNNRTG 1 cut(s) 726
Msp20I TGGCCA 1 cut(s) 588
MspA1I CMGCKG 1 cut(s) 651
MspCI CTTAAG 1 cut(s) 827
MspI CCGG 1 cut(s) 572
MspR9I CCNGG 2 cut(s) 585, 667
MunI CAATTG 1 cut(s) 501
MvaI CCWGG 2 cut(s) 585, 667
MwoI GCNNNNNNNGC 5 cut(s) 35, 204, 521, 523, 530
NcoI CCATGG 1 cut(s) 576
NdeI CATATG 1 cut(s) 520
NdeII GATC 5 cut(s) 333, 429, 543, 594, 822
NlaIII CATG 4 cut(s) 178, 250, 580, 595
NlaIV GGNNCC 5 cut(s) 431, 582, 812, 846, 871
NmeAIII GCCGAG 3 cut(s) 11, 88, 528
NsbI TGCGCA 1 cut(s) 525
NspI RCATGY 1 cut(s) 250
NspV TTCGAA 1 cut(s) 343
PceI AGGCCT 1 cut(s) 29
PciI ACATGT 1 cut(s) 246
PfeI GAWTC 1 cut(s) 340
PflMI CCANNNNNTGG 2 cut(s) 426, 695
PkrI GCNGC 5 cut(s) 6, 37, 197, 514, 624
PleI GAGTC 2 cut(s) 664, 863
PpsI GAGTC 2 cut(s) 664, 863
PscI ACATGT 1 cut(s) 246
Psp6I CCWGG 2 cut(s) 583, 665
PspGI CCWGG 2 cut(s) 583, 665
PspN4I GGNNCC 5 cut(s) 431, 582, 812, 846, 871
PspPI GGNCC 3 cut(s) 43, 296, 569
PsuI RGATCY 2 cut(s) 429, 822
RsaI GTAC 3 cut(s) 461, 491, 812
RsaNI GTAC 3 cut(s) 460, 490, 811
RseI CAYNNNNRTG 1 cut(s) 726
SaqAI TTAA 2 cut(s) 828, 882
SatI GCNGC 5 cut(s) 5, 36, 196, 513, 623
Sau3AI GATC 5 cut(s) 333, 429, 543, 594, 822
Sau96I GGNCC 3 cut(s) 43, 296, 569
SchI GAGTC 2 cut(s) 664, 864
ScrFI CCNGG 2 cut(s) 585, 667
SetI ASST 9 cut(s) 54, 59, 108, 126, 183, 270, 309, 773, 850
SfaNI GCATC 2 cut(s) 366, 561
SfuI TTCGAA 1 cut(s) 343
SinI GGWCC 1 cut(s) 569
SmiMI CAYNNNNRTG 1 cut(s) 726
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
Sse9I AATT 2 cut(s) 501, 751
SseBI AGGCCT 1 cut(s) 29
SsiI CCGC 5 cut(s) 35, 195, 205, 622, 651
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 2 cut(s) 583, 665
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 1 cut(s) 72
TaiI ACGT 2 cut(s) 108, 270
TaqI TCGA 4 cut(s) 21, 201, 343, 475
TasI AATT 2 cut(s) 501, 751
TatI WGTACW 1 cut(s) 489
TauI GCSGC 3 cut(s) 38, 198, 625
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 2 cut(s) 828, 882
Tru9I TTAA 2 cut(s) 828, 882
TscAI CASTG 3 cut(s) 75, 499, 768
TseI GCWGC 2 cut(s) 4, 512
TspDTI ATGAA 2 cut(s) 163, 277
TspRI CASTG 3 cut(s) 75, 499, 768
Van91I CCANNNNNTGG 2 cut(s) 426, 695
Vha464I CTTAAG 1 cut(s) 827
VpaK11BI GGWCC 1 cut(s) 569
XceI RCATGY 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 2 cut(s) 525, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.