MD07G1008300.v1.1

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004031
Physical Location & Seq
Forward (+)
778091 .. 780172
2082 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1008300.v1.1.491

Sequence Viewer

Length: 846 bp
ATGTTGCCTTATCGGAATATTGAAGAAGCAGAAGAAGCACTTAGTAGGGCTCTAAGTTTTGCAGAGAAGCTATGGTTCAAATACTCAGCTAAAAAACCAGATCACATCCTCCATTACCATAACATTATCTTCCTTATGTTGTTTTACACCTTGGCTCCCCTGCCGTACGTATTCATCAGCCTCGGCGGGTTCAAGAACATGGACAAGTACAAAATTCAGCCAAAAGTGAAGGACTCCTTCTTCAACATGTTCAAGTGCTACAAAGATGTCATGCAAACCTTCCTTCTTGTTGTTGGTCCTCTGCAAGTGATTTCATCCTCCACAGTCCAGTGGATAGGGATACGGACAAGTCTGCCATTGCCGTACTTGTCGCAAGTATTTGTGCAAATTGTGGTGTATTTCATTGTAGAGGATTTCTCACATTATTGGATCCATCGGATGCTCCACTGTAAATGGGCCTACGAGAAGATCCACCGGGTCCACCATGAATATACTGCTCCCATTGGGCTTGCAGCACCATACGCTCATTGGGCCGAGATTTTGATTCTGGGCCTTCCATCATTCCTCGGCCCAGCTTTGGTTCCTTGTCACATTACCACCTACTGGTTATGGTTCGTTTTGCGGCAGTTGGAAGCAATCGAAACTCATAGCGGGTACAACTTCCCTTGGAGTCCCACAAAATTTATCCCATTTTATGGAGGTGCAAAGTATCATGACTACCATCACTATGTCGGAGGACGTAGCAAAAGCAACTTTGCATCAGTTTTCACCTACTGCGATTACATCTACGGGACCCACAAGGGATACCAGTCTCACAAACAAGTACTTGAGAAGGCAAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
FA_hydroxylase

Protein Analysis

282

Amino Acids

33.04

Weight (kDa)

9.1

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 131 - 265 5.9e-28 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 603, 695
AciI CCGC 3 cut(s) 186, 622, 651
AclWI GGATC 3 cut(s) 424, 437, 463
AcsI RAATTY 2 cut(s) 213, 680
AfaI GTAC 5 cut(s) 167, 209, 365, 656, 825
AfiI CCNNNNNNNGG 3 cut(s) 577, 603, 695
AflIII ACRYGT 1 cut(s) 246
AgsI TTSAA 5 cut(s) 23, 79, 193, 244, 253
AluBI AGCT 3 cut(s) 70, 89, 575
AluI AGCT 3 cut(s) 70, 89, 575
Alw26I GTCTC 1 cut(s) 816
AlwI GGATC 3 cut(s) 424, 437, 463
AoxI GGCC 4 cut(s) 456, 531, 550, 568
ApeKI GCWGC 1 cut(s) 512
ApoI RAATTY 2 cut(s) 213, 680
AspS9I GGNCC 7 cut(s) 296, 456, 478, 531, 550, 569, 792
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 1 cut(s) 760
AvaII GGWCC 3 cut(s) 296, 478, 792
BamHI GGATCC 1 cut(s) 429
BanII GRGCYC 1 cut(s) 52
BbvI GCAGC 1 cut(s) 524
BccI CCATC 3 cut(s) 441, 565, 729
BceAI ACGGC 2 cut(s) 148, 346
BciVI GTATCC 2 cut(s) 333, 797
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 1 cut(s) 816
BfuI GTATCC 2 cut(s) 333, 797
BisI GCNGC 2 cut(s) 513, 623
BlsI GCNGC 2 cut(s) 514, 624
BmcAI AGTACT 1 cut(s) 825
Bme1390I CCNGG 1 cut(s) 476
Bme18I GGWCC 3 cut(s) 296, 478, 792
BmgT120I GGNCC 7 cut(s) 296, 456, 478, 531, 550, 569, 792
BmiI GGNNCC 6 cut(s) 156, 431, 479, 582, 793, 794
BmrFI CCNGG 1 cut(s) 476
BmsI GCATC 2 cut(s) 429, 767
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpuMI CCSGG 1 cut(s) 476
BsaAI YACGTR 1 cut(s) 169
BsaJI CCNNGG 4 cut(s) 150, 181, 565, 665
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 3 cut(s) 577, 603, 695
Bse1I ACTGG 3 cut(s) 328, 608, 808
Bse3DI GCAATG 1 cut(s) 356
BseDI CCNNGG 4 cut(s) 150, 181, 565, 665
BseGI GGATG 3 cut(s) 105, 314, 444
BseLI CCNNNNNNNGG 3 cut(s) 577, 603, 695
BseMI GCAATG 1 cut(s) 356
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 3 cut(s) 328, 608, 808
BseXI GCAGC 1 cut(s) 524
BseYI CCCAGC 1 cut(s) 571
BshFI GGCC 4 cut(s) 458, 533, 552, 570
BsiSI CCGG 1 cut(s) 475
BsiWI CGTACG 1 cut(s) 165
BslFI GGGAC 2 cut(s) 657, 805
BslI CCNNNNNNNGG 3 cut(s) 577, 603, 695
BsmAI GTCTC 1 cut(s) 816
BsmFI GGGAC 2 cut(s) 657, 805
BsnI GGCC 4 cut(s) 458, 533, 552, 570
Bsp1286I GDGCHC 1 cut(s) 52
Bsp143I GATC 3 cut(s) 100, 429, 468
BspACI CCGC 3 cut(s) 186, 622, 651
BspANI GGCC 4 cut(s) 458, 533, 552, 570
BspCNI CTCAG 1 cut(s) 98
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 6 cut(s) 156, 431, 479, 582, 793, 794
BspPI GGATC 3 cut(s) 424, 437, 463
BsrDI GCAATG 1 cut(s) 356
BsrI ACTGG 3 cut(s) 328, 608, 808
BssECI CCNNGG 4 cut(s) 150, 181, 565, 665
BssMI GATC 3 cut(s) 100, 429, 468
BssT1I CCWWGG 2 cut(s) 150, 665
Bst4CI ACNGT 3 cut(s) 325, 449, 843
BstBAI YACGTR 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 3 cut(s) 41, 53, 85
BstF5I GGATG 3 cut(s) 105, 314, 444
BstKTI GATC 3 cut(s) 103, 432, 471
BstMAI GTCTC 1 cut(s) 816
BstMBI GATC 3 cut(s) 100, 429, 468
BstMWI GCNNNNNNNGC 3 cut(s) 35, 521, 530
BstNSI RCATGY 1 cut(s) 250
BstSCI CCNGG 1 cut(s) 474
BstSNI TACGTA 1 cut(s) 169
BstV1I GCAGC 1 cut(s) 524
BstX2I RGATCY 2 cut(s) 429, 468
BstYI RGATCY 2 cut(s) 429, 468
BsuI GTATCC 2 cut(s) 333, 797
BsuRI GGCC 4 cut(s) 458, 533, 552, 570
BtsCI GGATG 3 cut(s) 105, 314, 444
BtsIMutI CAGTG 2 cut(s) 335, 445
Cac8I GCNNGC 1 cut(s) 510
CciI TCATGA 1 cut(s) 712
Cfr13I GGNCC 7 cut(s) 296, 456, 478, 531, 550, 569, 792
Csp6I GTAC 5 cut(s) 166, 208, 364, 655, 824
CviAII CATG 5 cut(s) 199, 247, 271, 485, 713
CviQI GTAC 5 cut(s) 166, 208, 364, 655, 824
DdeI CTNAG 3 cut(s) 41, 53, 85
DpnI GATC 3 cut(s) 102, 431, 470
DpnII GATC 3 cut(s) 100, 429, 468
Eco105I TACGTA 1 cut(s) 169
Eco130I CCWWGG 2 cut(s) 150, 665
Eco24I GRGCYC 1 cut(s) 52
Eco47I GGWCC 3 cut(s) 296, 478, 792
EcoO109I RGGNCCY 1 cut(s) 792
EcoT14I CCWWGG 2 cut(s) 150, 665
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 150, 665
FaeI CATG 5 cut(s) 202, 250, 274, 488, 716
FalI AAGNNNNNCTT 4 cut(s) 24, 56, 221, 253
FaqI GGGAC 2 cut(s) 657, 805
FatI CATG 5 cut(s) 198, 246, 270, 484, 712
FauI CCCGC 2 cut(s) 179, 644
Fnu4HI GCNGC 2 cut(s) 513, 623
FokI GGATG 3 cut(s) 92, 301, 451
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 2 cut(s) 513, 623
GluI GCNGC 2 cut(s) 513, 623
GsaI CCCAGC 1 cut(s) 575
HaeIII GGCC 4 cut(s) 458, 533, 552, 570
HapII CCGG 1 cut(s) 475
Hin1II CATG 5 cut(s) 202, 250, 274, 488, 716
HinfI GANTC 3 cut(s) 233, 544, 670
HpaII CCGG 1 cut(s) 475
HphI GGTGA 1 cut(s) 760
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 3 cut(s) 15, 438, 734
Hpy188III TCNNGA 2 cut(s) 193, 713
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 6 cut(s) 223, 247, 289, 293, 563, 826
HpyCH4III ACNGT 3 cut(s) 325, 449, 843
HpyCH4IV ACGT 2 cut(s) 168, 739
HpyCH4V TGCA 7 cut(s) 62, 274, 304, 385, 512, 704, 758
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 521, 530
HpyF3I CTNAG 3 cut(s) 41, 53, 85
HpySE526I ACGT 2 cut(s) 168, 739
Hsp92II CATG 5 cut(s) 202, 250, 274, 488, 716
KflI GGGWCCC 1 cut(s) 792
Kzo9I GATC 3 cut(s) 100, 429, 468
LmnI GCTCC 3 cut(s) 160, 447, 502
LpnPI CCDG 8 cut(s) 111, 173, 341, 488, 533, 585, 589, 821
Lsp1109I GCAGC 1 cut(s) 524
LweI GCATC 2 cut(s) 429, 767
MaeII ACGT 2 cut(s) 168, 739
MaeIII GTNAC 1 cut(s) 587
MalI GATC 3 cut(s) 102, 431, 470
MboI GATC 3 cut(s) 100, 429, 468
MboII GAAGA 5 cut(s) 35, 44, 121, 232, 478
MflI RGATCY 2 cut(s) 429, 468
MhlI GDGCHC 1 cut(s) 52
MluCI AATT 3 cut(s) 213, 387, 680
MlyI GAGTC 2 cut(s) 227, 679
MmeI TCCRAC 2 cut(s) 609, 712
MnlI CCTC 8 cut(s) 119, 191, 309, 328, 403, 575, 692, 728
MslI CAYNNNNRTG 1 cut(s) 726
MspI CCGG 1 cut(s) 475
MspR9I CCNGG 1 cut(s) 476
MwoI GCNNNNNNNGC 3 cut(s) 35, 521, 530
NciI CCSGG 1 cut(s) 476
NdeII GATC 3 cut(s) 100, 429, 468
NlaIII CATG 5 cut(s) 202, 250, 274, 488, 716
NlaIV GGNNCC 6 cut(s) 156, 431, 479, 582, 793, 794
NmeAIII GCCGAG 3 cut(s) 162, 546, 559
NmuCI GTSAC 1 cut(s) 587
NspI RCATGY 1 cut(s) 250
PagI TCATGA 1 cut(s) 712
PciI ACATGT 1 cut(s) 246
PfeI GAWTC 1 cut(s) 544
Pfl23II CGTACG 1 cut(s) 165
PflMI CCANNNNNTGG 2 cut(s) 603, 695
PkrI GCNGC 2 cut(s) 514, 624
PleI GAGTC 2 cut(s) 227, 678
PpsI GAGTC 2 cut(s) 227, 678
Ppu21I YACGTR 1 cut(s) 169
PpuMI RGGWCCY 1 cut(s) 792
PscI ACATGT 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 792
PspFI CCCAGC 1 cut(s) 571
PspLI CGTACG 1 cut(s) 165
PspN4I GGNNCC 6 cut(s) 156, 431, 479, 582, 793, 794
PspPI GGNCC 7 cut(s) 296, 456, 478, 531, 550, 569, 792
PspPPI RGGWCCY 1 cut(s) 792
PsuI RGATCY 2 cut(s) 429, 468
RsaI GTAC 5 cut(s) 167, 209, 365, 656, 825
RsaNI GTAC 5 cut(s) 166, 208, 364, 655, 824
RseI CAYNNNNRTG 1 cut(s) 726
SatI GCNGC 2 cut(s) 513, 623
Sau3AI GATC 3 cut(s) 100, 429, 468
Sau96I GGNCC 7 cut(s) 296, 456, 478, 531, 550, 569, 792
ScaI AGTACT 1 cut(s) 825
SchI GAGTC 2 cut(s) 227, 679
ScrFI CCNGG 1 cut(s) 476
SduI GDGCHC 1 cut(s) 52
SfaNI GCATC 2 cut(s) 429, 767
SinI GGWCC 3 cut(s) 296, 478, 792
SmiMI CAYNNNNRTG 1 cut(s) 726
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
SnaBI TACGTA 1 cut(s) 169
Sse9I AATT 3 cut(s) 213, 387, 680
SsiI CCGC 3 cut(s) 186, 622, 651
SspI AATATT 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 474
StyI CCWWGG 2 cut(s) 150, 665
TaaI ACNGT 3 cut(s) 325, 449, 843
TaiI ACGT 2 cut(s) 171, 742
TaqI TCGA 1 cut(s) 639
TasI AATT 3 cut(s) 213, 387, 680
TatI WGTACW 2 cut(s) 207, 823
TauI GCSGC 1 cut(s) 625
TfiI GAWTC 1 cut(s) 544
TscAI CASTG 2 cut(s) 335, 452
TseFI GTSAC 1 cut(s) 587
TseI GCWGC 1 cut(s) 512
Tsp45I GTSAC 1 cut(s) 587
TspDTI ATGAA 4 cut(s) 163, 303, 391, 501
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 2 cut(s) 335, 452
Van91I CCANNNNNTGG 2 cut(s) 603, 695
VpaK11BI GGWCC 3 cut(s) 296, 478, 792
XapI RAATTY 2 cut(s) 213, 680
XceI RCATGY 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 1 cut(s) 525
ZrmI AGTACT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.