MD04G1095900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011460
Physical Location & Seq
Reverse (-)
17666641 .. 17670325
3685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1095900.v1.1.491

Sequence Viewer

Length: 1386 bp
ATGAGCGATCAGCATCTACGGCTGAACCACCTCCGCAGCACCTCCCAGCTCCTCCGACAAGCCACGACGTCGTTCGCTGCCAACCTCTTCACCTTCGTCTTCCTCTCCCTCCTCATCTTCTCCTTCCGCACGGTCGTCGAAAATGGGACCCACCTCCTCACCTCCTTCGTCGACCGCGACCCTTCTCTCAAATCCCTCCTCTCCCGCCTCGACCTCGCTGGCGCCGGCAATACCAACCACCGCTCTCCCAGATCTCCAGCCGAGTCCCCTTCTCCAATCACTCTCCGCCGCCGACACCGCCCAGTGCTTCACCTCACCCGCGTCGGAACCTTAGACGATGATTTCTTCTCCGGCGATGATGACGACGCTCGCTCCCTCTTCGGCCCCAATCGGATCGCCCCAATCCTCATTTTCTCCTCCTCCTCTCACTCCACCTCCAAATTAGGGTTTAATGGAACTTACGGGACTAGGGTTTCTGAAATTGTGCGCTCCAGCCTCACGTTCAAGGCCGAGAAATTCACCATTTCCAATGACAGATCTAGAGGTAGGGATGGCGACGGAGAAAGCAATGAAGGGGAGGAGAAAGGCGGCGACGAAGAAATGGACGATCGGGCGGTCGATTTGCAATTCTTGATCAACGGATTGGAACTGGGTCGCCGGGACGTGGCGACGTTGTTCTTCCTTGTGAGTTTCTTATCTGCAGCTTACGGTTGGGTAATTCTAGGGTTTCTTGTAACGTATTCATGGGTTCTTGGCATTGTTTTTACAACAGTTGTGAATGATCTGATCGGGCGGTTTACTTCGTTTTTTGGTCTGGTTTGGGATGGGTCCAGATTGGGAATCAAGAGGCTCTCTGGGTTCATTCTCATGAGGTGGGCGGTGAGAGATGCTCTCACTCAGCTTCTGGGATTGTGGTATTTCGGCGAAATTGAGGACCAGTACTCTTTTTTCAAGCTGTTTATTAGGTTAAAGTTGATGCCTTTTTCGGTTATGCCTCCCTGGATTCGAGGTTATGAGAAGGAGCTTACTGGGTTTTTGTTTGTGTGGTACTTGTTGGACACATTGGTGGCATTCGTATTTGCTGTCGATGCTTGGGTAGCTATAGTGGATTCGAGGAAGAGTGGGAGAGAAATTGTCAAGGAGGGCTGTTATTTAATGTTGTCAATGTTGAGCCAAGCTATCCAAATCAAGTGTTTGGAATCGGTACTTTGTGGGGGATCTGTAAGATGGGTTTTAACTCGAATTTGTGGAAGATCGTTTGCCAAGCTCTTTCAGTCGACTGTTGAAGTTTATTTTATGGTATCCTGGCTCATATTTTACTTTGCAGCTAGGGCGAGGTGTCGAGATGCTAGTTCTCAGGGGGAGAGGATTGGAGCACTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

462

Amino Acids

52.08

Weight (kDa)

8.75

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7813 PF25105 10 - 459 7.4e-199 Family of unknown function (DUF7813)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37035
fragaria_vesca FvH4_6g16100
malus_domestica MD04G1095900.v1.1 MD12G1115900.v1.1
prunus_persica Prupe.6G235000_v2.0.a1
pyrus_communis pycom04g09080 pycom12g11540 pycom15g07040
rosa_chinensis RchiOBHm_Chr3g0469941
rosa_laevigata RLG00000024311
rosa_multiflora Rmu_sc0008347.1_g000001
rosa_roxburghii Rroxscaffold_5G00354980 Rroxscaffold_6G00411350
rosa_rugosa Rorug03G0105600 Rorug05G0154500
rosa_samantha Rh3AG154500 Rh3BG178600 Rh3CG169200 Rh3DG196500
rosa_wichuraiana Rw3G014560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 243
AccI GTMKAC 2 cut(s) 171, 1277
AccII CGCG 2 cut(s) 177, 321
AclWI GGATC 2 cut(s) 401, 1225
AcsI RAATTY 2 cut(s) 515, 1242
AcyI GRCGYC 2 cut(s) 68, 222
AfaI GTAC 3 cut(s) 941, 1049, 1206
AfiI CCNNNNNNNGG 2 cut(s) 444, 664
AgsI TTSAA 3 cut(s) 505, 952, 1286
AjiI CACGTC 1 cut(s) 664
AjnI CCWGG 2 cut(s) 998, 1304
AleI CACNNNNGTG 1 cut(s) 1064
AluBI AGCT 9 cut(s) 49, 704, 901, 955, 1024, 1100, 1178, 1267, 1328
AluI AGCT 9 cut(s) 49, 704, 901, 955, 1024, 1100, 1178, 1267, 1328
Alw21I GWGCWC 1 cut(s) 1378
AlwI GGATC 2 cut(s) 401, 1225
AlwNI CAGNNNCTG 1 cut(s) 904
AoxI GGCC 2 cut(s) 382, 507
ApeKI GCWGC 4 cut(s) 36, 77, 701, 1325
ApoI RAATTY 2 cut(s) 515, 1242
AspLEI GCGC 2 cut(s) 224, 489
AspS9I GGNCC 4 cut(s) 147, 383, 828, 934
AsuC2I CCSGG 1 cut(s) 659
AsuHPI GGTGA 6 cut(s) 82, 151, 302, 307, 511, 892
AvaII GGWCC 3 cut(s) 147, 828, 934
BanI GGYRCC 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 91
Bbv12I GWGCWC 1 cut(s) 1378
BbvI GCAGC 4 cut(s) 48, 64, 713, 1337
BccI CCATC 3 cut(s) 545, 818, 1221
BceAI ACGGC 1 cut(s) 35
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 2 cut(s) 1000, 1306
BciVI GTATCC 1 cut(s) 1312
BclI TGATCA 1 cut(s) 633
BcnI CCSGG 1 cut(s) 659
BfaI CTAG 6 cut(s) 468, 540, 722, 1329, 1350, 1379
BfmI CTRYAG 2 cut(s) 699, 1101
BfoI RGCGCY 1 cut(s) 225
BfuI GTATCC 1 cut(s) 1312
BglII AGATCT 2 cut(s) 251, 536
BisI GCNGC 6 cut(s) 37, 78, 289, 589, 702, 1326
BlsI GCNGC 6 cut(s) 38, 79, 290, 590, 703, 1327
BmcAI AGTACT 1 cut(s) 941
Bme1390I CCNGG 3 cut(s) 659, 1000, 1306
Bme18I GGWCC 3 cut(s) 147, 828, 934
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 4 cut(s) 147, 383, 828, 934
BmiI GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 829
BmrFI CCNGG 3 cut(s) 659, 1000, 1306
BmrI ACTGGG 3 cut(s) 296, 659, 1038
BmsI GCATC 5 cut(s) 22, 877, 966, 1078, 1336
BmuI ACTGGG 3 cut(s) 296, 659, 1038
BpiI GAAGAC 1 cut(s) 91
BplI GAGNNNNNCTC 4 cut(s) 874, 906, 876, 908
BpmI CTGGAG 2 cut(s) 240, 475
BpuMI CCSGG 1 cut(s) 659
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 2 cut(s) 68, 222
BsaJI CCNNGG 1 cut(s) 998
BsaXI ACNNNNNCTCC 4 cut(s) 356, 386, 419, 449
Bsc4I CCNNNNNNNGG 2 cut(s) 444, 664
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 4 cut(s) 302, 654, 937, 1033
Bse3DI GCAATG 1 cut(s) 574
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 1000, 1306
BseDI CCNNGG 1 cut(s) 998
BseGI GGATG 2 cut(s) 556, 829
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 444, 664
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 2 cut(s) 911, 1370
BseNI ACTGG 4 cut(s) 302, 654, 937, 1033
BseRI GAGGAG 8 cut(s) 41, 101, 146, 188, 406, 409, 412, 593
BseXI GCAGC 4 cut(s) 48, 64, 713, 1337
BseYI CCCAGC 1 cut(s) 45
Bsh1236I CGCG 2 cut(s) 177, 321
Bsh1285I CGRYCG 4 cut(s) 135, 175, 610, 618
BshFI GGCC 2 cut(s) 384, 509
BshNI GGYRCC 1 cut(s) 221
BsiEI CGRYCG 4 cut(s) 135, 175, 610, 618
BsiHKAI GWGCWC 1 cut(s) 1378
BsiSI CCGG 3 cut(s) 225, 351, 658
BslFI GGGAC 4 cut(s) 160, 250, 478, 674
BslI CCNNNNNNNGG 2 cut(s) 444, 664
BsmFI GGGAC 4 cut(s) 160, 250, 478, 674
BsmI GAATGC 1 cut(s) 1070
BsnI GGCC 2 cut(s) 384, 509
Bsp1286I GDGCHC 1 cut(s) 1378
BspANI GGCC 2 cut(s) 384, 509
BspCNI CTCAG 2 cut(s) 910, 1369
BspFNI CGCG 2 cut(s) 177, 321
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 829
BspMAI CTGCAG 1 cut(s) 703
BspPI GGATC 2 cut(s) 401, 1225
BspT107I GGYRCC 1 cut(s) 221
BsrBI CCGCTC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 574
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 4 cut(s) 302, 654, 937, 1033
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 998
BssNI GRCGYC 2 cut(s) 68, 222
Bst2UI CCWGG 2 cut(s) 1000, 1306
Bst4CI ACNGT 4 cut(s) 133, 710, 772, 1282
Bst6I CTCTTC 3 cut(s) 92, 383, 1112
BstACI GRCGYC 2 cut(s) 68, 222
BstC8I GCNNGC 3 cut(s) 220, 226, 370
BstDEI CTNAG 3 cut(s) 331, 897, 1356
BstF5I GGATG 2 cut(s) 556, 829
BstFNI CGCG 2 cut(s) 177, 321
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 2 cut(s) 224, 489
BstMCI CGRYCG 4 cut(s) 135, 175, 610, 618
BstMWI GCNNNNNNNGC 5 cut(s) 19, 297, 1088, 1097, 1331
BstNI CCWGG 2 cut(s) 1000, 1306
BstSCI CCNGG 3 cut(s) 657, 998, 1304
BstSFI CTRYAG 2 cut(s) 699, 1101
BstUI CGCG 2 cut(s) 177, 321
BstV1I GCAGC 4 cut(s) 48, 64, 713, 1337
BstV2I GAAGAC 1 cut(s) 91
BstX2I RGATCY 3 cut(s) 251, 536, 1217
BstYI RGATCY 3 cut(s) 251, 536, 1217
BsuI GTATCC 1 cut(s) 1312
BsuRI GGCC 2 cut(s) 384, 509
BtgZI GCGATG 1 cut(s) 369
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 2 cut(s) 556, 829
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 3 cut(s) 220, 226, 370
CaiI CAGNNNCTG 1 cut(s) 904
CciI TCATGA 1 cut(s) 867
CfoI GCGC 2 cut(s) 224, 489
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 4 cut(s) 147, 383, 828, 934
CseI GACGC 2 cut(s) 310, 374
Csp6I GTAC 3 cut(s) 940, 1048, 1205
CviAII CATG 2 cut(s) 744, 868
CviQI GTAC 3 cut(s) 940, 1048, 1205
DdeI CTNAG 3 cut(s) 331, 897, 1356
DinI GGCGCC 1 cut(s) 223
Eam1104I CTCTTC 3 cut(s) 92, 383, 1112
EarI CTCTTC 3 cut(s) 92, 383, 1112
EciI GGCGGA 1 cut(s) 275
Eco47I GGWCC 3 cut(s) 147, 828, 934
EcoO109I RGGNCCY 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 998, 1304
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
FaeI CATG 2 cut(s) 747, 871
FaiI YATR 7 cut(s) 745, 869, 992, 1014, 1103, 1298, 1313
FaqI GGGAC 4 cut(s) 160, 250, 478, 674
FatI CATG 2 cut(s) 743, 867
FauI CCCGC 2 cut(s) 212, 326
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 2 cut(s) 171, 1277
Fnu4HI GCNGC 6 cut(s) 37, 78, 289, 589, 702, 1326
FokI GGATG 2 cut(s) 563, 836
Fsp4HI GCNGC 6 cut(s) 37, 78, 289, 589, 702, 1326
FspBI CTAG 6 cut(s) 468, 540, 722, 1329, 1350, 1379
GlaI GCGC 2 cut(s) 223, 488
GluI GCNGC 6 cut(s) 37, 78, 289, 589, 702, 1326
GsaI CCCAGC 1 cut(s) 49
GsuI CTGGAG 2 cut(s) 240, 475
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 2 cut(s) 384, 509
HapII CCGG 3 cut(s) 225, 351, 658
HgaI GACGC 2 cut(s) 310, 374
HhaI GCGC 2 cut(s) 224, 489
Hin1I GRCGYC 2 cut(s) 68, 222
Hin1II CATG 2 cut(s) 747, 871
Hin6I GCGC 2 cut(s) 222, 487
HinP1I GCGC 2 cut(s) 222, 487
HincII GTYRAC 2 cut(s) 172, 1278
HindII GTYRAC 2 cut(s) 172, 1278
HinfI GANTC 5 cut(s) 263, 840, 1003, 1109, 1199
HpaII CCGG 3 cut(s) 225, 351, 658
HphI GGTGA 6 cut(s) 82, 151, 302, 307, 511, 892
Hpy166II GTNNAC 3 cut(s) 172, 798, 1278
Hpy188I TCNGA 5 cut(s) 56, 326, 393, 478, 786
Hpy188III TCNNGA 6 cut(s) 540, 631, 831, 844, 868, 1343
Hpy8I GTNNAC 3 cut(s) 172, 798, 1278
Hpy99I CGWCG 9 cut(s) 70, 73, 140, 173, 326, 368, 560, 596, 673
HpyAV CCTTC 7 cut(s) 103, 133, 175, 192, 279, 566, 1012
HpyCH4III ACNGT 4 cut(s) 133, 710, 772, 1282
HpyCH4IV ACGT 5 cut(s) 68, 500, 663, 671, 737
HpyCH4V TGCA 3 cut(s) 625, 701, 1325
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 297, 1088, 1097, 1331
HpyF3I CTNAG 3 cut(s) 331, 897, 1356
HpySE526I ACGT 5 cut(s) 68, 500, 663, 671, 737
Hsp92I GRCGYC 2 cut(s) 68, 222
Hsp92II CATG 2 cut(s) 747, 871
HspAI GCGC 2 cut(s) 222, 487
KasI GGCGCC 1 cut(s) 221
KflI GGGWCCC 1 cut(s) 147
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 633
LmnI GCTCC 5 cut(s) 54, 377, 494, 1021, 1373
Lsp1109I GCAGC 4 cut(s) 48, 64, 713, 1337
LweI GCATC 5 cut(s) 22, 877, 966, 1078, 1336
MaeI CTAG 6 cut(s) 468, 540, 722, 1329, 1350, 1379
MaeII ACGT 5 cut(s) 68, 500, 663, 671, 737
MaeIII GTNAC 1 cut(s) 733
MbiI CCGCTC 1 cut(s) 243
MboII GAAGA 9 cut(s) 79, 91, 109, 337, 370, 608, 670, 1129, 1263
MflI RGATCY 3 cut(s) 251, 536, 1217
MhlI GDGCHC 1 cut(s) 1378
MluCI AATT 8 cut(s) 440, 480, 515, 626, 717, 927, 1131, 1242
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 3 cut(s) 79, 304, 1035
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 4 cut(s) 450, 968, 1154, 1235
MslI CAYNNNNRTG 2 cut(s) 866, 1064
MspI CCGG 3 cut(s) 225, 351, 658
MspR9I CCNGG 3 cut(s) 659, 1000, 1306
Mva1269I GAATGC 1 cut(s) 1070
MvaI CCWGG 2 cut(s) 1000, 1306
MvnI CGCG 2 cut(s) 177, 321
MwoI GCNNNNNNNGC 5 cut(s) 19, 297, 1088, 1097, 1331
NaeI GCCGGC 1 cut(s) 226
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 1 cut(s) 659
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 2 cut(s) 747, 871
NlaIV GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 829
NmeAIII GCCGAG 2 cut(s) 286, 535
OliI CACNNNNGTG 1 cut(s) 1064
PagI TCATGA 1 cut(s) 867
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 1 cut(s) 1070
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 4 cut(s) 840, 1003, 1109, 1199
PkrI GCNGC 6 cut(s) 38, 79, 290, 590, 703, 1327
Ple19I CGATCG 1 cut(s) 610
PleI GAGTC 1 cut(s) 271
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 147
Psp6I CCWGG 2 cut(s) 998, 1304
PspFI CCCAGC 1 cut(s) 45
PspGI CCWGG 2 cut(s) 998, 1304
PspN4I GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 829
PspPI GGNCC 4 cut(s) 147, 383, 828, 934
PspPPI RGGWCCY 1 cut(s) 147
PstI CTGCAG 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 904
PsuI RGATCY 3 cut(s) 251, 536, 1217
PvuI CGATCG 1 cut(s) 610
RsaI GTAC 3 cut(s) 941, 1049, 1206
RsaNI GTAC 3 cut(s) 940, 1048, 1205
RseI CAYNNNNRTG 2 cut(s) 866, 1064
SalI GTCGAC 2 cut(s) 170, 1276
SaqAI TTAA 4 cut(s) 450, 968, 1154, 1235
SatI GCNGC 6 cut(s) 37, 78, 289, 589, 702, 1326
Sau96I GGNCC 4 cut(s) 147, 383, 828, 934
ScaI AGTACT 1 cut(s) 941
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 3 cut(s) 659, 1000, 1306
SduI GDGCHC 1 cut(s) 1378
SfaNI GCATC 5 cut(s) 22, 877, 966, 1078, 1336
SfcI CTRYAG 2 cut(s) 699, 1101
SfoI GGCGCC 1 cut(s) 223
SinI GGWCC 3 cut(s) 147, 828, 934
SmiMI CAYNNNNRTG 2 cut(s) 866, 1064
Sse9I AATT 8 cut(s) 440, 480, 515, 626, 717, 927, 1131, 1242
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 6 cut(s) 468, 540, 722, 1329, 1350, 1379
StyD4I CCNGG 3 cut(s) 657, 998, 1304
TaaI ACNGT 4 cut(s) 133, 710, 772, 1282
TaiI ACGT 5 cut(s) 71, 503, 666, 674, 740
TasI AATT 8 cut(s) 440, 480, 515, 626, 717, 927, 1131, 1242
TatI WGTACW 1 cut(s) 939
TauI GCSGC 2 cut(s) 291, 591
TfiI GAWTC 4 cut(s) 840, 1003, 1109, 1199
Tru1I TTAA 4 cut(s) 450, 968, 1154, 1235
Tru9I TTAA 4 cut(s) 450, 968, 1154, 1235
TscAI CASTG 1 cut(s) 309
TseI GCWGC 4 cut(s) 36, 77, 701, 1325
TspDTI ATGAA 3 cut(s) 585, 732, 850
TspGWI ACGGA 2 cut(s) 573, 654
TspRI CASTG 1 cut(s) 309
VpaK11BI GGWCC 3 cut(s) 147, 828, 934
XapI RAATTY 2 cut(s) 515, 1242
XbaI TCTAGA 1 cut(s) 539
XmiI GTMKAC 2 cut(s) 171, 1277
XspI CTAG 6 cut(s) 468, 540, 722, 1329, 1350, 1379
ZraI GACGTC 1 cut(s) 69
ZrmI AGTACT 1 cut(s) 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.