pycom12g11540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011460
Physical Location & Seq
Reverse (-)
13756968 .. 13758353
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11540.1

Sequence Viewer

Length: 1386 bp
ATGAGCGATCAGCATCCACGGTTGAACCACCTCCGCAGCACCTCTCAGCTCCTCCGACAAGCCACGACGTCGTTCGCCGGCAACCTCTTCACCTTCGTCTTCCTCTCCCTCCTCATCTTCTCCTTCCGGACGGTCGTCGAAAATGGGACCCACCTCCTCACAACCTTCGTCGACCGCGACCCTTCTCTCAAATCCCTCCTCTCCCGCCTCGACCTCGCTGGAGCCGGAAATCCCAACCACCGGTCTCCCCGATCTCAGGCCGAGTCCCCTTCTCCGATCACTCTCCGCCGCCGACACCGCCCAGTTCTCCACCTCACCCGGGTCGGAACCTTAGACGAGGATTTCTTCTCCGGTGATGACGACGACGCCCGATCCCTCTTCGGCTCCAATCGGATCGCCCCCATCCTCATTTTCTCCTCCTCCTCTCACTCCACCTCCAAATTAGGGTTTAATGGAACTTACGGGACTAGGGTTTCTGAGATCATGCGCTCCAGCCTCACGTTCAAGGCCGAGAAATTCACCTTATCCGATGATAGTGCTAGAGACAGAGATAGCGACGGAGATAGCAATGACCGAGAAGAGAAAGGGGGCGATGACGAAATGGACGATCGGGCTGTCGATTTGCAATTCTTCATCAAGGGATTGGAGCTAGGTCGCCAGGACGTGGCGACGTTGTTCTTCCTTGTGAGTTTCTTATCTGCAGCTTACGGTTGGGTAATTCTAGGGTTTCTTGTAACGTATTCATGGGTTCTTGGCATTGTTTTTACCACAGTTGTGAATGATCTGATCGGGCGGTTTACTTCGTTTATTGGTCTGGTTTGGGATGGGTCCAGATTCGGCATCAAGAGGCTCTCTGGGTTCATTCTCATGAGGTGGGCGGTGAGAGATGCTCTCACTCAGCTTCTGGGATTGTGGTATTTCGGCGAAATTGAGGACCAGTACTCTTTTTTCAAGCTATTTATCAGGTTAAAGTTGATGCCTTTTTCGGTTATGCCTCCCTGGATTCGAGGTTATGAGAAGGAGATTACTGGGTTTTTGTTTATGTGGTACTGGCTGGATACATTGGTGGCATTCGTATTTTCTGTCGATGCATGGGTTGCCATAGTGGATTCAAGGAAGAGTGGGAAAGAGATTGTCAAGGAAGGCTGTCATTTAATGTTGTCAATGTTGAGCCAAGCTATCCAAATTAAGTGTTTAGAATCTGTATTGTGTGGGGAATCTGTAAGATGGGCTTTAACTCGAATTTGTGGAAGATCGTTTGCCAAGCTCTTTCAGTCGACTGTGGAAGTTTACTTTATGGTTGCTTGGCTCATATTTTACTTTGCAGCGAGGGCGAGGAGTAGAGATGCTAGTTCTCAGGGGGAGAGGATTGGAGCCCTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

462

Amino Acids

52.29

Weight (kDa)

7.72

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7813 PF25105 10 - 459 1.2e-195 Family of unknown function (DUF7813)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37035
fragaria_vesca FvH4_6g16100
malus_domestica MD04G1095900.v1.1 MD12G1115900.v1.1
prunus_persica Prupe.6G235000_v2.0.a1
pyrus_communis pycom04g09080 pycom12g11540 pycom15g07040
rosa_chinensis RchiOBHm_Chr3g0469941
rosa_laevigata RLG00000024311
rosa_multiflora Rmu_sc0008347.1_g000001
rosa_roxburghii Rroxscaffold_5G00354980 Rroxscaffold_6G00411350
rosa_rugosa Rorug03G0105600 Rorug05G0154500
rosa_samantha Rh3AG154500 Rh3BG178600 Rh3CG169200 Rh3DG196500
rosa_wichuraiana Rw3G014560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 664
AccI GTMKAC 2 cut(s) 171, 1277
AccII CGCG 1 cut(s) 177
AccIII TCCGGA 1 cut(s) 126
AciI CCGC 8 cut(s) 34, 175, 205, 286, 289, 298, 793, 878
AclWI GGATC 2 cut(s) 366, 401
AcsI RAATTY 2 cut(s) 515, 1242
AcyI GRCGYC 2 cut(s) 68, 366
AfaI GTAC 2 cut(s) 941, 1049
AfiI CCNNNNNNNGG 5 cut(s) 240, 256, 319, 444, 664
AgeI ACCGGT 1 cut(s) 240
AgsI TTSAA 4 cut(s) 25, 505, 952, 1113
AjiI CACGTC 1 cut(s) 664
AjnI CCWGG 2 cut(s) 657, 998
AleI CACNNNNGTG 1 cut(s) 773
AluBI AGCT 7 cut(s) 49, 649, 704, 901, 955, 1178, 1267
AluI AGCT 7 cut(s) 49, 649, 704, 901, 955, 1178, 1267
Alw26I GTCTC 2 cut(s) 249, 537
AlwI GGATC 2 cut(s) 366, 401
AlwNI CAGNNNCTG 1 cut(s) 904
Ama87I CYCGRG 1 cut(s) 318
Aor13HI TCCGGA 1 cut(s) 126
AoxI GGCC 2 cut(s) 258, 507
ApeKI GCWGC 3 cut(s) 36, 701, 1325
ApoI RAATTY 2 cut(s) 515, 1242
AsiGI ACCGGT 1 cut(s) 240
AspLEI GCGC 1 cut(s) 489
AspS9I GGNCC 3 cut(s) 147, 828, 934
AsuC2I CCSGG 2 cut(s) 319, 320
AsuHPI GGTGA 5 cut(s) 82, 307, 365, 511, 892
AvaI CYCGRG 1 cut(s) 318
AvaII GGWCC 3 cut(s) 147, 828, 934
BaeI ACNNNNGTAYC 2 cut(s) 1050, 1083
BanII GRGCYC 1 cut(s) 1378
BbsI GAAGAC 1 cut(s) 91
BbvI GCAGC 3 cut(s) 48, 713, 1337
BccI CCATC 3 cut(s) 410, 818, 1221
BcgI CGANNNNNNTGC 2 cut(s) 518, 552
BciT130I CCWGG 2 cut(s) 659, 1000
BciVI GTATCC 1 cut(s) 1051
BcnI CCSGG 2 cut(s) 319, 320
BcoDI GTCTC 2 cut(s) 249, 537
BfaI CTAG 6 cut(s) 468, 540, 650, 722, 1350, 1379
BfmI CTRYAG 1 cut(s) 699
BfuI GTATCC 1 cut(s) 1051
BisI GCNGC 4 cut(s) 37, 289, 702, 1326
BlsI GCNGC 4 cut(s) 38, 290, 703, 1327
BmcAI AGTACT 1 cut(s) 941
Bme1390I CCNGG 4 cut(s) 319, 320, 659, 1000
Bme18I GGWCC 3 cut(s) 147, 828, 934
BmeT110I CYCGRG 1 cut(s) 318
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 3 cut(s) 147, 828, 934
BmiI GGNNCC 7 cut(s) 148, 149, 223, 328, 385, 829, 1375
BmrFI CCNGG 4 cut(s) 319, 320, 659, 1000
BmrI ACTGGG 2 cut(s) 296, 1038
BmsI GCATC 6 cut(s) 22, 849, 877, 966, 1078, 1336
BmuI ACTGGG 2 cut(s) 296, 1038
BoxI GACNNNNGTC 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 91
BplI GAGNNNNNCTC 4 cut(s) 874, 906, 876, 908
BpmI CTGGAG 2 cut(s) 240, 475
BpuMI CCSGG 2 cut(s) 319, 320
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 2 cut(s) 68, 366
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 3 cut(s) 17, 318, 998
BsaWI WCCGGW 3 cut(s) 126, 240, 350
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 5 cut(s) 240, 256, 319, 444, 664
Bse118I RCCGGY 2 cut(s) 77, 240
Bse1I ACTGG 4 cut(s) 302, 937, 1033, 1055
Bse3DI GCAATG 1 cut(s) 574
Bse8I GATNNNNATC 1 cut(s) 12
BseAI TCCGGA 1 cut(s) 126
BseBI CCWGG 2 cut(s) 659, 1000
BseDI CCNNGG 3 cut(s) 17, 318, 998
BseGI GGATG 3 cut(s) 13, 402, 829
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 5 cut(s) 240, 256, 319, 444, 664
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 5 cut(s) 59, 269, 468, 911, 1370
BseNI ACTGG 4 cut(s) 302, 937, 1033, 1055
BseRI GAGGAG 8 cut(s) 41, 101, 146, 188, 406, 409, 412, 1351
BseXI GCAGC 3 cut(s) 48, 713, 1337
Bsh1236I CGCG 1 cut(s) 177
Bsh1285I CGRYCG 3 cut(s) 135, 175, 610
BshFI GGCC 2 cut(s) 260, 509
BshTI ACCGGT 1 cut(s) 240
BsiEI CGRYCG 3 cut(s) 135, 175, 610
BsiHKCI CYCGRG 1 cut(s) 318
BsiSI CCGG 6 cut(s) 78, 127, 225, 241, 319, 351
BslFI GGGAC 3 cut(s) 160, 250, 478
BslI CCNNNNNNNGG 5 cut(s) 240, 256, 319, 444, 664
BsmAI GTCTC 2 cut(s) 249, 537
BsmFI GGGAC 3 cut(s) 160, 250, 478
BsmI GAATGC 1 cut(s) 1070
BsnI GGCC 2 cut(s) 260, 509
Bso31I GGTCTC 1 cut(s) 249
BsoBI CYCGRG 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 1378
Bsp13I TCCGGA 1 cut(s) 126
BspACI CCGC 8 cut(s) 34, 175, 205, 286, 289, 298, 793, 878
BspANI GGCC 2 cut(s) 260, 509
BspCNI CTCAG 5 cut(s) 58, 268, 469, 910, 1369
BspEI TCCGGA 1 cut(s) 126
BspFNI CGCG 1 cut(s) 177
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 7 cut(s) 148, 149, 223, 328, 385, 829, 1375
BspMAI CTGCAG 1 cut(s) 703
BspPI GGATC 2 cut(s) 366, 401
BspTNI GGTCTC 1 cut(s) 249
BsrDI GCAATG 1 cut(s) 574
BsrFI RCCGGY 2 cut(s) 77, 240
BsrI ACTGG 4 cut(s) 302, 937, 1033, 1055
BssAI RCCGGY 2 cut(s) 77, 240
BssECI CCNNGG 3 cut(s) 17, 318, 998
BssNI GRCGYC 2 cut(s) 68, 366
Bst2UI CCWGG 2 cut(s) 659, 1000
Bst4CI ACNGT 5 cut(s) 21, 133, 710, 772, 1282
Bst6I CTCTTC 4 cut(s) 92, 383, 573, 1112
BstACI GRCGYC 2 cut(s) 68, 366
BstAPI GCANNNNNTGC 1 cut(s) 1097
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 6 cut(s) 45, 255, 331, 477, 897, 1356
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 3 cut(s) 13, 402, 829
BstFNI CGCG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 489
BstMAI GTCTC 2 cut(s) 249, 537
BstMCI CGRYCG 3 cut(s) 135, 175, 610
BstMWI GCNNNNNNNGC 3 cut(s) 297, 1097, 1331
BstNI CCWGG 2 cut(s) 659, 1000
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 4 cut(s) 317, 318, 657, 998
BstSFI CTRYAG 1 cut(s) 699
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 3 cut(s) 48, 713, 1337
BstV2I GAAGAC 1 cut(s) 91
BsuI GTATCC 1 cut(s) 1051
BsuRI GGCC 2 cut(s) 260, 509
BtgI CCRYGG 1 cut(s) 17
BtgZI GCGATG 1 cut(s) 606
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 3 cut(s) 13, 402, 829
Cac8I GCNNGC 1 cut(s) 79
CaiI CAGNNNCTG 1 cut(s) 904
CciI TCATGA 1 cut(s) 867
CfoI GCGC 1 cut(s) 489
Cfr10I RCCGGY 2 cut(s) 77, 240
Cfr13I GGNCC 3 cut(s) 147, 828, 934
Cfr9I CCCGGG 1 cut(s) 318
CseI GACGC 1 cut(s) 374
Csp6I GTAC 2 cut(s) 940, 1048
CspAI ACCGGT 1 cut(s) 240
CviAII CATG 4 cut(s) 484, 744, 868, 1092
CviQI GTAC 2 cut(s) 940, 1048
DdeI CTNAG 6 cut(s) 45, 255, 331, 477, 897, 1356
Eam1104I CTCTTC 4 cut(s) 92, 383, 573, 1112
EarI CTCTTC 4 cut(s) 92, 383, 573, 1112
EciI GGCGGA 1 cut(s) 275
Eco24I GRGCYC 1 cut(s) 1378
Eco31I GGTCTC 1 cut(s) 249
Eco47I GGWCC 3 cut(s) 147, 828, 934
Eco88I CYCGRG 1 cut(s) 318
EcoO109I RGGNCCY 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 657, 998
EcoT22I ATGCAT 1 cut(s) 1093
EcoT38I GRGCYC 1 cut(s) 1378
FaeI CATG 4 cut(s) 487, 747, 871, 1095
FalI AAGNNNNNCTT 2 cut(s) 1216, 1248
FaqI GGGAC 3 cut(s) 160, 250, 478
FatI CATG 4 cut(s) 483, 743, 867, 1091
FauI CCCGC 1 cut(s) 212
FblI GTMKAC 2 cut(s) 171, 1277
Fnu4HI GCNGC 4 cut(s) 37, 289, 702, 1326
FokI GGATG 2 cut(s) 389, 836
FriOI GRGCYC 1 cut(s) 1378
Fsp4HI GCNGC 4 cut(s) 37, 289, 702, 1326
FspBI CTAG 6 cut(s) 468, 540, 650, 722, 1350, 1379
GlaI GCGC 1 cut(s) 488
GluI GCNGC 4 cut(s) 37, 289, 702, 1326
GsuI CTGGAG 2 cut(s) 240, 475
HaeIII GGCC 2 cut(s) 260, 509
HapII CCGG 6 cut(s) 78, 127, 225, 241, 319, 351
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 489
Hin1I GRCGYC 2 cut(s) 68, 366
Hin1II CATG 4 cut(s) 487, 747, 871, 1095
Hin6I GCGC 1 cut(s) 487
HinP1I GCGC 1 cut(s) 487
HincII GTYRAC 2 cut(s) 172, 1278
HindII GTYRAC 2 cut(s) 172, 1278
HinfI GANTC 6 cut(s) 263, 834, 1003, 1109, 1199, 1217
HpaII CCGG 6 cut(s) 78, 127, 225, 241, 319, 351
HphI GGTGA 5 cut(s) 82, 307, 365, 511, 892
Hpy166II GTNNAC 4 cut(s) 172, 798, 1278, 1291
Hpy188I TCNGA 7 cut(s) 56, 276, 326, 393, 478, 529, 786
Hpy188III TCNNGA 4 cut(s) 127, 831, 844, 868
Hpy8I GTNNAC 4 cut(s) 172, 798, 1278, 1291
Hpy99I CGWCG 8 cut(s) 70, 73, 140, 173, 365, 368, 560, 673
HpyAV CCTTC 7 cut(s) 103, 133, 175, 192, 279, 1012, 1136
HpyCH4III ACNGT 5 cut(s) 21, 133, 710, 772, 1282
HpyCH4IV ACGT 5 cut(s) 68, 500, 663, 671, 737
HpyCH4V TGCA 4 cut(s) 625, 701, 1091, 1325
HpyF10VI GCNNNNNNNGC 3 cut(s) 297, 1097, 1331
HpyF3I CTNAG 6 cut(s) 45, 255, 331, 477, 897, 1356
HpySE526I ACGT 5 cut(s) 68, 500, 663, 671, 737
Hsp92I GRCGYC 2 cut(s) 68, 366
Hsp92II CATG 4 cut(s) 487, 747, 871, 1095
HspAI GCGC 1 cut(s) 487
KflI GGGWCCC 1 cut(s) 147
Kpn2I TCCGGA 1 cut(s) 126
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
LmnI GCTCC 6 cut(s) 54, 221, 389, 494, 646, 1373
Lsp1109I GCAGC 3 cut(s) 48, 713, 1337
LweI GCATC 6 cut(s) 22, 849, 877, 966, 1078, 1336
MaeI CTAG 6 cut(s) 468, 540, 650, 722, 1350, 1379
MaeII ACGT 5 cut(s) 68, 500, 663, 671, 737
MaeIII GTNAC 1 cut(s) 733
MhlI GDGCHC 1 cut(s) 1378
MluCI AATT 7 cut(s) 440, 515, 626, 717, 927, 1185, 1242
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 2 cut(s) 79, 304
Mph1103I ATGCAT 1 cut(s) 1093
MroI TCCGGA 1 cut(s) 126
MroNI GCCGGC 1 cut(s) 77
MseI TTAA 5 cut(s) 450, 968, 1154, 1188, 1235
MslI CAYNNNNRTG 2 cut(s) 773, 866
MspI CCGG 6 cut(s) 78, 127, 225, 241, 319, 351
MspR9I CCNGG 4 cut(s) 319, 320, 659, 1000
Mva1269I GAATGC 1 cut(s) 1070
MvaI CCWGG 2 cut(s) 659, 1000
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 3 cut(s) 297, 1097, 1331
NaeI GCCGGC 1 cut(s) 79
NciI CCSGG 2 cut(s) 319, 320
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 4 cut(s) 487, 747, 871, 1095
NlaIV GGNNCC 7 cut(s) 148, 149, 223, 328, 385, 829, 1375
NmeAIII GCCGAG 2 cut(s) 286, 535
NsiI ATGCAT 1 cut(s) 1093
OliI CACNNNNGTG 1 cut(s) 773
PagI TCATGA 1 cut(s) 867
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 603
PctI GAATGC 1 cut(s) 1070
PdiI GCCGGC 1 cut(s) 79
PfeI GAWTC 5 cut(s) 834, 1003, 1109, 1199, 1217
PflMI CCANNNNNTGG 1 cut(s) 664
PinAI ACCGGT 1 cut(s) 240
PkrI GCNGC 4 cut(s) 38, 290, 703, 1327
Ple19I CGATCG 1 cut(s) 610
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 147
PshAI GACNNNNGTC 1 cut(s) 134
Psp5II RGGWCCY 1 cut(s) 147
Psp6I CCWGG 2 cut(s) 657, 998
PspGI CCWGG 2 cut(s) 657, 998
PspN4I GGNNCC 7 cut(s) 148, 149, 223, 328, 385, 829, 1375
PspPI GGNCC 3 cut(s) 147, 828, 934
PspPPI RGGWCCY 1 cut(s) 147
PstI CTGCAG 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 904
PvuI CGATCG 1 cut(s) 610
RsaI GTAC 2 cut(s) 941, 1049
RsaNI GTAC 2 cut(s) 940, 1048
RseI CAYNNNNRTG 2 cut(s) 773, 866
SalI GTCGAC 2 cut(s) 170, 1276
SaqAI TTAA 5 cut(s) 450, 968, 1154, 1188, 1235
SatI GCNGC 4 cut(s) 37, 289, 702, 1326
Sau96I GGNCC 3 cut(s) 147, 828, 934
ScaI AGTACT 1 cut(s) 941
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 319, 320, 659, 1000
SduI GDGCHC 1 cut(s) 1378
SfaNI GCATC 6 cut(s) 22, 849, 877, 966, 1078, 1336
SfcI CTRYAG 1 cut(s) 699
SinI GGWCC 3 cut(s) 147, 828, 934
SmaI CCCGGG 1 cut(s) 320
SmiMI CAYNNNNRTG 2 cut(s) 773, 866
Sse9I AATT 7 cut(s) 440, 515, 626, 717, 927, 1185, 1242
SsiI CCGC 8 cut(s) 34, 175, 205, 286, 289, 298, 793, 878
SspMI CTAG 6 cut(s) 468, 540, 650, 722, 1350, 1379
StyD4I CCNGG 4 cut(s) 317, 318, 657, 998
TaaI ACNGT 5 cut(s) 21, 133, 710, 772, 1282
TaiI ACGT 5 cut(s) 71, 503, 666, 674, 740
TaqI TCGA 8 cut(s) 138, 171, 210, 618, 1006, 1086, 1240, 1277
TaqII GACCGA 1 cut(s) 588
TasI AATT 7 cut(s) 440, 515, 626, 717, 927, 1185, 1242
TatI WGTACW 1 cut(s) 939
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 5 cut(s) 834, 1003, 1109, 1199, 1217
Tru1I TTAA 5 cut(s) 450, 968, 1154, 1188, 1235
Tru9I TTAA 5 cut(s) 450, 968, 1154, 1188, 1235
TseI GCWGC 3 cut(s) 36, 701, 1325
TspDTI ATGAA 3 cut(s) 622, 732, 850
TspGWI ACGGA 1 cut(s) 573
TspMI CCCGGG 1 cut(s) 318
Van91I CCANNNNNTGG 1 cut(s) 664
VpaK11BI GGWCC 3 cut(s) 147, 828, 934
XapI RAATTY 2 cut(s) 515, 1242
XmaI CCCGGG 1 cut(s) 318
XmiI GTMKAC 2 cut(s) 171, 1277
XspI CTAG 6 cut(s) 468, 540, 650, 722, 1350, 1379
ZraI GACGTC 1 cut(s) 69
ZrmI AGTACT 1 cut(s) 941
Zsp2I ATGCAT 1 cut(s) 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.