Prupe.6G235000_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0011460
Physical Location & Seq
Reverse (-)
23625383 .. 23627450
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G235000.1

Sequence Viewer

Length: 1386 bp
ATGAGCGATCAGCATCCACGGCTGAACCACCTGCGCAGCACCGCCCAAGTCCTGAGACAAGCGACGACGTCGTTTGCCGGCAACCTCTTCACCTTCTTCTTCCTCTCTCTCCTCATCTTCTCCTTCCGTACGGTCGTCGAAAATGGGACCTACCTCCTCACATCCTTTATCGACCGCGACCCTTCTCTCAAGTCCCTCCTCTCCCGCCTCGATCTCGCCGGCGCTGCCAGCCCCAACCACCGCTCTTCCCGATCTCCGGCGGATTCCCCTTCTCCGGTCACCCTCCGCCGCCGCCACCGTCCATTTCTCCACCTCACCCGAGTCGGAACCCTAGACGACGACTTCTTCTCCGGCGACGACGGCGACACTCGCTCCCAATTCGGCCCCAATCGCATCGCCCCTATCCTCATTTTCTCCTCCGCCTCTCACTCCACCTCCAAATTAGGGTTTAATGGAACTTACGGAACTAGGGTTTCTGAAATTGTCCGCTCCGGTCTCACGTTCAAAGCCGATAAATTCACCATTTCCGATGACAGAGCTGGAGAGGGAGATAGGGAAGGCAATAGCAGTGAAGGAGAGGAGAAAGGCGGTGATGAGGAATTGGATGATCGGGTCGTCGATTTGCAATTCTTCATCAAGGGATTGGAGCTGGGTCGCCGAGATGTGGCGACGTTGTTCTTTCTTGTGAGTTTCTTATCTGCAGCTTACGGTTGGGTAATTCTTGGGTTTCTTATTACGTATTCATGGGTTCTTGGCGTTGTGTTTACCACAGTTGTGAATGATCTGATCGGGAGGATTACTTCGTTTGTTGGTCTGGTTTGGGATGGGTCTAGACTGGGCGTCAAGAGGCTCTCTGGGTTCATTCTCATGAGGTGGGCGGTGAGAGATGCTCTCACTCAGCTTCTCGGCTTGTGGTATTTCGGGGAAATTGAGGACCAGTACTCATTTTTCAAACTTTTCATCAGGTTAAAGTTGATGCCTTTTTCGGTTATGCCTCCGTGGATTCGAGGTTATGAGAAGGAGATTTCTGGGTTTCTGTTAATGTGGTTCGTGTTGGATACATTGGTGGCATTCGTATTCGCTGTCGATGCTTGGGTTGCCATAGTGGATTCGAGGAAGAATGGAAGGGAGATTGTCAAGGAAGGATGTTATTTGATGCTGTCAATGTTGCGCCAAGCTATCCAGATTAAGTGTTTGGAAGCTATATTTTGTGGGTCATCTGTAAGATGGATTGTAGCTCGAATTTGTGGAAAATCTTTTGCCAAGCTTTTTCAGGCAACTGTGGAAGTCTACTTTATGGTGGCTTGGCTGATATTTTACTTTGCAGCGAGGGCGAGGTGTAGGGATGCTAGTTCTCAGGGGGATAGGATCGGAGCTCTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

462

Amino Acids

51.93

Weight (kDa)

8.89

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37035
fragaria_vesca FvH4_6g16100
malus_domestica MD04G1095900.v1.1 MD12G1115900.v1.1
prunus_persica Prupe.6G235000_v2.0.a1
pyrus_communis pycom04g09080 pycom12g11540 pycom15g07040
rosa_chinensis RchiOBHm_Chr3g0469941
rosa_laevigata RLG00000024311
rosa_multiflora Rmu_sc0008347.1_g000001
rosa_roxburghii Rroxscaffold_5G00354980 Rroxscaffold_6G00411350
rosa_rugosa Rorug03G0105600 Rorug05G0154500
rosa_samantha Rh3AG154500 Rh3BG178600 Rh3CG169200 Rh3DG196500
rosa_wichuraiana Rw3G014560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 39
AatII GACGTC 1 cut(s) 71
Acc16I TGCGCA 1 cut(s) 35
Acc36I ACCTGC 1 cut(s) 39
AccBSI CCGCTC 2 cut(s) 243, 489
AccI GTMKAC 1 cut(s) 1290
AccII CGCG 1 cut(s) 177
AclWI GGATC 1 cut(s) 1376
AcsI RAATTY 2 cut(s) 515, 1242
AcyI GRCGYC 2 cut(s) 68, 840
AfaI GTAC 2 cut(s) 130, 941
AfiI CCNNNNNNNGG 4 cut(s) 256, 274, 444, 664
AgsI TTSAA 2 cut(s) 505, 952
AhdI GACNNNNNGTC 1 cut(s) 839
AleI CACNNNNGTG 1 cut(s) 773
AluBI AGCT 9 cut(s) 539, 649, 704, 901, 1178, 1202, 1238, 1267, 1376
AluI AGCT 9 cut(s) 539, 649, 704, 901, 1178, 1202, 1238, 1267, 1376
Alw21I GWGCWC 1 cut(s) 1378
Alw26I GTCTC 2 cut(s) 49, 500
AlwI GGATC 1 cut(s) 1376
Ama87I CYCGRG 1 cut(s) 318
AoxI GGCC 1 cut(s) 382
ApeKI GCWGC 4 cut(s) 36, 224, 701, 1325
ApoI RAATTY 2 cut(s) 515, 1242
AspLEI GCGC 3 cut(s) 36, 224, 1173
AspS9I GGNCC 3 cut(s) 147, 383, 934
AsuHPI GGTGA 6 cut(s) 82, 271, 307, 511, 602, 892
AvaI CYCGRG 1 cut(s) 318
AvaII GGWCC 2 cut(s) 147, 934
BaeI ACNNNNGTAYC 2 cut(s) 1050, 1083
BanII GRGCYC 1 cut(s) 1378
Bbv12I GWGCWC 1 cut(s) 1378
BbvI GCAGC 4 cut(s) 48, 211, 713, 1337
BccI CCATC 2 cut(s) 818, 1221
BceAI ACGGC 2 cut(s) 35, 376
BciVI GTATCC 1 cut(s) 1051
BcoDI GTCTC 2 cut(s) 49, 500
BfaI CTAG 5 cut(s) 332, 468, 831, 1350, 1379
BfmI CTRYAG 1 cut(s) 699
BfoI RGCGCY 1 cut(s) 225
BfuAI ACCTGC 1 cut(s) 39
BfuI GTATCC 1 cut(s) 1051
BisI GCNGC 6 cut(s) 37, 225, 289, 292, 702, 1326
BlsI GCNGC 6 cut(s) 38, 226, 290, 293, 703, 1327
BmcAI AGTACT 1 cut(s) 941
Bme18I GGWCC 2 cut(s) 147, 934
BmeRI GACNNNNNGTC 1 cut(s) 839
BmeT110I CYCGRG 1 cut(s) 318
BmgT120I GGNCC 3 cut(s) 147, 383, 934
BmiI GGNNCC 3 cut(s) 148, 328, 385
BmrI ACTGGG 1 cut(s) 845
BmsI GCATC 7 cut(s) 22, 402, 877, 966, 1078, 1146, 1336
BmuI ACTGGG 1 cut(s) 845
BplI GAGNNNNNCTC 4 cut(s) 874, 906, 876, 908
BpmI CTGGAG 1 cut(s) 561
BpuEI CTTGAG 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 738
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 2 cut(s) 68, 840
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 2 cut(s) 17, 998
BsaWI WCCGGW 2 cut(s) 274, 491
BsaXI ACNNNNNCTCC 6 cut(s) 332, 356, 362, 386, 419, 449
Bsc4I CCNNNNNNNGG 4 cut(s) 256, 274, 444, 664
Bse118I RCCGGY 2 cut(s) 77, 218
Bse1I ACTGG 2 cut(s) 840, 937
Bse8I GATNNNNATC 1 cut(s) 12
BseDI CCNNGG 2 cut(s) 17, 998
BseGI GGATG 6 cut(s) 13, 161, 610, 829, 1151, 1351
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 4 cut(s) 256, 274, 444, 664
BseMII CTCAG 3 cut(s) 44, 911, 1370
BseNI ACTGG 2 cut(s) 840, 937
BseRI GAGGAG 5 cut(s) 101, 146, 188, 406, 593
BseXI GCAGC 4 cut(s) 48, 211, 713, 1337
BseYI CCCAGC 1 cut(s) 649
Bsh1236I CGCG 1 cut(s) 177
Bsh1285I CGRYCG 2 cut(s) 135, 175
BshFI GGCC 1 cut(s) 384
BsiEI CGRYCG 2 cut(s) 135, 175
BsiHKAI GWGCWC 1 cut(s) 1378
BsiHKCI CYCGRG 1 cut(s) 318
BsiSI CCGG 6 cut(s) 78, 219, 257, 275, 351, 492
BsiWI CGTACG 1 cut(s) 128
BslFI GGGAC 2 cut(s) 160, 178
BslI CCNNNNNNNGG 4 cut(s) 256, 274, 444, 664
BsmAI GTCTC 2 cut(s) 49, 500
BsmFI GGGAC 2 cut(s) 160, 178
BsmI GAATGC 1 cut(s) 1070
BsnI GGCC 1 cut(s) 384
Bso31I GGTCTC 1 cut(s) 500
BsoBI CYCGRG 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 1378
Bsp143I GATC 7 cut(s) 7, 211, 251, 607, 781, 786, 1368
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 3 cut(s) 45, 910, 1369
BspFNI CGCG 1 cut(s) 177
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 3 cut(s) 148, 328, 385
BspMAI CTGCAG 1 cut(s) 703
BspMI ACCTGC 1 cut(s) 39
BspPI GGATC 1 cut(s) 1376
BspQI GCTCTTC 1 cut(s) 250
BspTNI GGTCTC 1 cut(s) 500
BsrBI CCGCTC 2 cut(s) 243, 489
BsrFI RCCGGY 2 cut(s) 77, 218
BsrI ACTGG 2 cut(s) 840, 937
BssAI RCCGGY 2 cut(s) 77, 218
BssECI CCNNGG 2 cut(s) 17, 998
BssMI GATC 7 cut(s) 7, 211, 251, 607, 781, 786, 1368
BssNI GRCGYC 2 cut(s) 68, 840
Bst4CI ACNGT 5 cut(s) 133, 299, 710, 772, 1282
Bst6I CTCTTC 2 cut(s) 92, 250
BstACI GRCGYC 2 cut(s) 68, 840
BstBAI YACGTR 1 cut(s) 738
BstC8I GCNNGC 3 cut(s) 79, 220, 229
BstDEI CTNAG 3 cut(s) 53, 897, 1356
BstDSI CCRYGG 2 cut(s) 17, 998
BstEII GGTNACC 1 cut(s) 277
BstF5I GGATG 6 cut(s) 13, 161, 610, 829, 1151, 1351
BstFNI CGCG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 3 cut(s) 36, 224, 1173
BstKTI GATC 7 cut(s) 10, 214, 254, 610, 784, 789, 1371
BstMAI GTCTC 2 cut(s) 49, 500
BstMBI GATC 7 cut(s) 7, 211, 251, 607, 781, 786, 1368
BstMCI CGRYCG 2 cut(s) 135, 175
BstMWI GCNNNNNNNGC 9 cut(s) 19, 224, 228, 360, 369, 390, 1088, 1097, 1331
BstPI GGTNACC 1 cut(s) 277
BstSFI CTRYAG 1 cut(s) 699
BstSNI TACGTA 1 cut(s) 738
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 4 cut(s) 48, 211, 713, 1337
BsuI GTATCC 1 cut(s) 1051
BsuRI GGCC 1 cut(s) 384
BtgI CCRYGG 2 cut(s) 17, 998
BtgZI GCGATG 1 cut(s) 379
BtsCI GGATG 6 cut(s) 13, 161, 610, 829, 1151, 1351
BtsI GCAGTG 1 cut(s) 574
BtsIMutI CAGTG 1 cut(s) 574
BveI ACCTGC 1 cut(s) 39
Cac8I GCNNGC 3 cut(s) 79, 220, 229
CciI TCATGA 1 cut(s) 867
CfoI GCGC 3 cut(s) 36, 224, 1173
Cfr10I RCCGGY 2 cut(s) 77, 218
Cfr13I GGNCC 3 cut(s) 147, 383, 934
CseI GACGC 1 cut(s) 829
Csp6I GTAC 2 cut(s) 129, 940
CviAII CATG 2 cut(s) 744, 868
CviQI GTAC 2 cut(s) 129, 940
DdeI CTNAG 3 cut(s) 53, 897, 1356
DpnI GATC 7 cut(s) 9, 213, 253, 609, 783, 788, 1370
DpnII GATC 7 cut(s) 7, 211, 251, 607, 781, 786, 1368
DriI GACNNNNNGTC 1 cut(s) 839
Eam1104I CTCTTC 2 cut(s) 92, 250
Eam1105I GACNNNNNGTC 1 cut(s) 839
EarI CTCTTC 2 cut(s) 92, 250
EciI GGCGGA 3 cut(s) 275, 275, 409
Ecl136II GAGCTC 1 cut(s) 1376
Eco105I TACGTA 1 cut(s) 738
Eco24I GRGCYC 1 cut(s) 1378
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 2 cut(s) 147, 934
Eco53kI GAGCTC 1 cut(s) 1376
Eco88I CYCGRG 1 cut(s) 318
Eco91I GGTNACC 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 1376
EcoO109I RGGNCCY 1 cut(s) 147
EcoO65I GGTNACC 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 1378
FaeI CATG 2 cut(s) 747, 871
FaiI YATR 7 cut(s) 745, 869, 992, 1014, 1103, 1205, 1298
FaqI GGGAC 2 cut(s) 160, 178
FatI CATG 2 cut(s) 743, 867
FauI CCCGC 1 cut(s) 212
FblI GTMKAC 1 cut(s) 1290
Fnu4HI GCNGC 6 cut(s) 37, 225, 289, 292, 702, 1326
FokI GGATG 5 cut(s) 148, 617, 836, 1158, 1358
FriOI GRGCYC 1 cut(s) 1378
Fsp4HI GCNGC 6 cut(s) 37, 225, 289, 292, 702, 1326
FspBI CTAG 5 cut(s) 332, 468, 831, 1350, 1379
FspI TGCGCA 1 cut(s) 35
GlaI GCGC 3 cut(s) 35, 223, 1172
GluI GCNGC 6 cut(s) 37, 225, 289, 292, 702, 1326
GsaI CCCAGC 1 cut(s) 653
GsuI CTGGAG 1 cut(s) 561
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 1 cut(s) 384
HapII CCGG 6 cut(s) 78, 219, 257, 275, 351, 492
HgaI GACGC 1 cut(s) 829
HhaI GCGC 3 cut(s) 36, 224, 1173
Hin1I GRCGYC 2 cut(s) 68, 840
Hin1II CATG 2 cut(s) 747, 871
Hin6I GCGC 3 cut(s) 34, 222, 1171
HinP1I GCGC 3 cut(s) 34, 222, 1171
HindIII AAGCTT 1 cut(s) 1265
HinfI GANTC 4 cut(s) 263, 321, 1003, 1109
HpaII CCGG 6 cut(s) 78, 219, 257, 275, 351, 492
HphI GGTGA 6 cut(s) 82, 271, 307, 511, 602, 892
Hpy166II GTNNAC 2 cut(s) 765, 1291
Hpy188I TCNGA 5 cut(s) 326, 478, 529, 786, 1373
Hpy188III TCNNGA 8 cut(s) 52, 249, 790, 831, 844, 868, 1183, 1379
Hpy8I GTNNAC 2 cut(s) 765, 1291
Hpy99I CGWCG 9 cut(s) 67, 70, 73, 140, 341, 359, 362, 620, 673
HpyAV CCTTC 9 cut(s) 103, 133, 192, 279, 551, 566, 1012, 1119, 1136
HpyCH4III ACNGT 5 cut(s) 133, 299, 710, 772, 1282
HpyCH4IV ACGT 4 cut(s) 68, 500, 671, 737
HpyCH4V TGCA 3 cut(s) 625, 701, 1325
HpyF10VI GCNNNNNNNGC 9 cut(s) 19, 224, 228, 360, 369, 390, 1088, 1097, 1331
HpyF3I CTNAG 3 cut(s) 53, 897, 1356
HpySE526I ACGT 4 cut(s) 68, 500, 671, 737
Hsp92I GRCGYC 2 cut(s) 68, 840
Hsp92II CATG 2 cut(s) 747, 871
HspAI GCGC 3 cut(s) 34, 222, 1171
KroI GCCGGC 2 cut(s) 77, 218
KroNI GCCGGC 2 cut(s) 79, 220
Kzo9I GATC 7 cut(s) 7, 211, 251, 607, 781, 786, 1368
LguI GCTCTTC 1 cut(s) 250
LmnI GCTCC 4 cut(s) 377, 494, 646, 1373
Lsp1109I GCAGC 4 cut(s) 48, 211, 713, 1337
LweI GCATC 7 cut(s) 22, 402, 877, 966, 1078, 1146, 1336
MaeI CTAG 5 cut(s) 332, 468, 831, 1350, 1379
MaeII ACGT 4 cut(s) 68, 500, 671, 737
MaeIII GTNAC 1 cut(s) 277
MalI GATC 7 cut(s) 9, 213, 253, 609, 783, 788, 1370
MbiI CCGCTC 2 cut(s) 243, 489
MboI GATC 7 cut(s) 7, 211, 251, 607, 781, 786, 1368
MboII GAAGA 8 cut(s) 79, 88, 91, 109, 237, 337, 622, 1129
MhlI GDGCHC 1 cut(s) 1378
MluCI AATT 9 cut(s) 377, 440, 480, 515, 599, 626, 717, 927, 1242
MlyI GAGTC 1 cut(s) 330
MmeI TCCRAC 2 cut(s) 304, 1035
MreI CGCCGGCG 1 cut(s) 218
MroNI GCCGGC 2 cut(s) 77, 218
MseI TTAA 4 cut(s) 450, 968, 1040, 1188
MslI CAYNNNNRTG 2 cut(s) 773, 866
MspI CCGG 6 cut(s) 78, 219, 257, 275, 351, 492
Mva1269I GAATGC 1 cut(s) 1070
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 9 cut(s) 19, 224, 228, 360, 369, 390, 1088, 1097, 1331
NaeI GCCGGC 2 cut(s) 79, 220
NdeII GATC 7 cut(s) 7, 211, 251, 607, 781, 786, 1368
NgoMIV GCCGGC 2 cut(s) 77, 218
NlaIII CATG 2 cut(s) 747, 871
NlaIV GGNNCC 3 cut(s) 148, 328, 385
NmeAIII GCCGAG 2 cut(s) 683, 885
NmuCI GTSAC 1 cut(s) 277
NsbI TGCGCA 1 cut(s) 35
OliI CACNNNNGTG 1 cut(s) 773
PagI TCATGA 1 cut(s) 867
PaqCI CACCTGC 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 250
PctI GAATGC 1 cut(s) 1070
PdiI GCCGGC 2 cut(s) 79, 220
PfeI GAWTC 3 cut(s) 263, 1003, 1109
Pfl23II CGTACG 1 cut(s) 128
PflFI GACNNNGTC 1 cut(s) 67
PkrI GCNGC 6 cut(s) 38, 226, 290, 293, 703, 1327
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
Ppu21I YACGTR 1 cut(s) 738
PpuMI RGGWCCY 1 cut(s) 147
Psp124BI GAGCTC 1 cut(s) 1378
Psp5II RGGWCCY 1 cut(s) 147
PspEI GGTNACC 1 cut(s) 277
PspFI CCCAGC 1 cut(s) 649
PspLI CGTACG 1 cut(s) 128
PspN4I GGNNCC 3 cut(s) 148, 328, 385
PspPI GGNCC 3 cut(s) 147, 383, 934
PspPPI RGGWCCY 1 cut(s) 147
PstI CTGCAG 1 cut(s) 703
PsyI GACNNNGTC 1 cut(s) 67
RsaI GTAC 2 cut(s) 130, 941
RsaNI GTAC 2 cut(s) 129, 940
RseI CAYNNNNRTG 2 cut(s) 773, 866
SacI GAGCTC 1 cut(s) 1378
SapI GCTCTTC 1 cut(s) 250
SaqAI TTAA 4 cut(s) 450, 968, 1040, 1188
SatI GCNGC 6 cut(s) 37, 225, 289, 292, 702, 1326
Sau3AI GATC 7 cut(s) 7, 211, 251, 607, 781, 786, 1368
Sau96I GGNCC 3 cut(s) 147, 383, 934
ScaI AGTACT 1 cut(s) 941
SchI GAGTC 1 cut(s) 330
SduI GDGCHC 1 cut(s) 1378
SfaNI GCATC 7 cut(s) 22, 402, 877, 966, 1078, 1146, 1336
SfcI CTRYAG 1 cut(s) 699
SgrAI CRCCGGYG 1 cut(s) 218
SinI GGWCC 2 cut(s) 147, 934
SmiMI CAYNNNNRTG 2 cut(s) 773, 866
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SnaBI TACGTA 1 cut(s) 738
Sse9I AATT 9 cut(s) 377, 440, 480, 515, 599, 626, 717, 927, 1242
SspMI CTAG 5 cut(s) 332, 468, 831, 1350, 1379
SstI GAGCTC 1 cut(s) 1378
TaaI ACNGT 5 cut(s) 133, 299, 710, 772, 1282
TaiI ACGT 4 cut(s) 71, 503, 674, 740
TaqI TCGA 8 cut(s) 138, 171, 210, 618, 1006, 1086, 1112, 1240
TasI AATT 9 cut(s) 377, 440, 480, 515, 599, 626, 717, 927, 1242
TatI WGTACW 1 cut(s) 939
TauI GCSGC 2 cut(s) 291, 294
TfiI GAWTC 3 cut(s) 263, 1003, 1109
Tru1I TTAA 4 cut(s) 450, 968, 1040, 1188
Tru9I TTAA 4 cut(s) 450, 968, 1040, 1188
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 4 cut(s) 36, 224, 701, 1325
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 4 cut(s) 622, 732, 850, 949
TspGWI ACGGA 3 cut(s) 116, 477, 987
TspRI CASTG 1 cut(s) 574
Tth111I GACNNNGTC 1 cut(s) 67
VpaK11BI GGWCC 2 cut(s) 147, 934
XapI RAATTY 2 cut(s) 515, 1242
XbaI TCTAGA 2 cut(s) 830, 1378
XmiI GTMKAC 1 cut(s) 1290
XspI CTAG 5 cut(s) 332, 468, 831, 1350, 1379
ZraI GACGTC 1 cut(s) 69
ZrmI AGTACT 1 cut(s) 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.