pycom04g09080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011460
Physical Location & Seq
Reverse (-)
11358721 .. 11360106
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g09080.1

Sequence Viewer

Length: 1386 bp
ATGAGCGATCAGCATCCACGGCTGAACCACCTCCGCAGCACCTCCCAGCTCCTCCGACAAGCCACGACGTCGTTCGCTGGCAACCTTTTCACCTTCGTCTTCCTCTCCCTCCTCATCTTCTCCTTCCGCACGGTCGTCGAAAATGGGACCCACCTTCTCACTTCCTTCGTCGACCGCGACCCTTCTCTCAAATCCCTCCTCTCCCGCCTTGACCTCGCTGGCGCCGGCAATACCAACCACCGCTCTCCCAGATCTCCAGCCGAGACCCCTTCTCCAATCACTCTCCGCCGGCGACACCGTCCATTTCTTCACCTCACCCGCGTCGGAACCTTAGACGACGATTTCTTCTCCGGCGATGACGACGACGCTCGCTCCCTCTTCGGCCCCAATCGGATCGATCCAAAGCTCATTTTCTCCTCCTCCTCTCACTCCACCTCCAAATTAGGGTTTAATGGAACTTACGGGACTAGGGTTTCTGAAATCGTGCGCTCCAGCCTCACGTTCAAGGCCGGGAAATTCACCATTTCCGATGACAGAGCTAGAGGCAGAGATGGCGACGGAGAAAGCAATGAAGGGGAGGAGAAAGGCGGCGATGAAGAAATGGACGATCGGGCCGTCGATTTGCAATTCTTGATCAACGGATTGGAGCTGGGTCGCCGGGACGTGGCGACGTTGTTCTTCCTTGTGAGTTTCTTATCTGCAGCTTACGGTTGGGTAATTCTAGGGTTTCTTGTAACGTATTCATGGGTTCTTGGCATTGTTTTTACAACAGTTGTGAATGATCTGATCGGGCGGTTTACTTCGTTTTTTGGTCTGGTTTGGGATGGGTCCAGATTGGGCATCAAGAGGCTCTCTGGGTTCATTCTCATGAGGTGGGCGGTGAGAGATGCTCTCACTCAGCTTCTGGGATTGTGGTATTTCGGTGAAATTGAGGACCAGTACTCTTTTTTCAAGCTGTTTATTAGGTTAAAGTTGATGCCTTTTTCGGTTATGCCTCCCTGGATTCGAGGTTATGAGAAGGAGCTTACTGGGTTTTTGTTTGTGTGGTACTTGTTGGATACATTGGTGGCATTCGTATTTGCTGTCGATGCTTGGGTTGCTATAGTGGATTCGAGGAAGAGTGGGAGAGAAATTGTCAAGGAAGGCTGTTATTTAATGTTGTCAATGTTGAGCCAAGCTATCCAAATCAAGTGTTTGGAATCTGTACTTTGTGGGGGATCTGTAAGATGGGTTTTAACTCGAATTTGTGGAAGATCGTTTGCCAAGCTCTTTCAGTCGACTGTTGAAGTTTATTTTATGGTATCCTGGCTCATATTTTACTTTGCAGCTAGCGCGAGGTGTCGAGATGCTAGTTCTCAGGGGGAGAGGATTGGAGCACTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

462

Amino Acids

52.02

Weight (kDa)

8.53

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7813 PF25105 10 - 459 4.8e-199 Family of unknown function (DUF7813)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37035
fragaria_vesca FvH4_6g16100
malus_domestica MD04G1095900.v1.1 MD12G1115900.v1.1
prunus_persica Prupe.6G235000_v2.0.a1
pyrus_communis pycom04g09080 pycom12g11540 pycom15g07040
rosa_chinensis RchiOBHm_Chr3g0469941
rosa_laevigata RLG00000024311
rosa_multiflora Rmu_sc0008347.1_g000001
rosa_roxburghii Rroxscaffold_5G00354980 Rroxscaffold_6G00411350
rosa_rugosa Rorug03G0105600 Rorug05G0154500
rosa_samantha Rh3AG154500 Rh3BG178600 Rh3CG169200 Rh3DG196500
rosa_wichuraiana Rw3G014560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 243
AccI GTMKAC 2 cut(s) 171, 1277
AccII CGCG 3 cut(s) 177, 321, 1334
AclWI GGATC 3 cut(s) 392, 401, 1225
AcsI RAATTY 2 cut(s) 515, 1242
AcyI GRCGYC 2 cut(s) 68, 222
AfaI GTAC 3 cut(s) 941, 1049, 1206
AfiI CCNNNNNNNGG 2 cut(s) 444, 664
AgsI TTSAA 3 cut(s) 505, 952, 1286
AjiI CACGTC 1 cut(s) 664
AjnI CCWGG 2 cut(s) 998, 1304
Alw21I GWGCWC 1 cut(s) 1378
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 3 cut(s) 392, 401, 1225
AlwNI CAGNNNCTG 1 cut(s) 904
AoxI GGCC 3 cut(s) 382, 507, 612
ApeKI GCWGC 3 cut(s) 36, 701, 1325
ApoI RAATTY 2 cut(s) 515, 1242
AspLEI GCGC 3 cut(s) 224, 489, 1334
AspS9I GGNCC 5 cut(s) 147, 383, 612, 828, 934
AsuC2I CCSGG 2 cut(s) 511, 659
AsuHPI GGTGA 6 cut(s) 82, 302, 307, 511, 892, 935
AsuNHI GCTAGC 1 cut(s) 1328
AvaII GGWCC 3 cut(s) 147, 828, 934
BaeI ACNNNNGTAYC 2 cut(s) 1050, 1083
BanI GGYRCC 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 91
Bbv12I GWGCWC 1 cut(s) 1378
BbvI GCAGC 3 cut(s) 48, 713, 1337
BccI CCATC 3 cut(s) 545, 818, 1221
BceAI ACGGC 2 cut(s) 35, 599
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 2 cut(s) 1000, 1306
BciVI GTATCC 2 cut(s) 1051, 1312
BclI TGATCA 1 cut(s) 633
BcnI CCSGG 2 cut(s) 511, 659
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 6 cut(s) 468, 540, 722, 1329, 1350, 1379
BfmI CTRYAG 2 cut(s) 699, 1101
BfoI RGCGCY 1 cut(s) 225
BfuI GTATCC 2 cut(s) 1051, 1312
BglII AGATCT 1 cut(s) 251
BisI GCNGC 4 cut(s) 37, 589, 702, 1326
BlsI GCNGC 4 cut(s) 38, 590, 703, 1327
BmcAI AGTACT 1 cut(s) 941
Bme1390I CCNGG 4 cut(s) 511, 659, 1000, 1306
Bme18I GGWCC 3 cut(s) 147, 828, 934
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 5 cut(s) 147, 383, 612, 828, 934
BmiI GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 829
BmrFI CCNGG 4 cut(s) 511, 659, 1000, 1306
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 6 cut(s) 22, 849, 877, 966, 1078, 1336
BmtI GCTAGC 1 cut(s) 1332
BmuI ACTGGG 1 cut(s) 1038
BpiI GAAGAC 1 cut(s) 91
BplI GAGNNNNNCTC 4 cut(s) 874, 906, 876, 908
BpmI CTGGAG 2 cut(s) 240, 475
BpuMI CCSGG 2 cut(s) 511, 659
Bsa29I ATCGAT 1 cut(s) 396
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 2 cut(s) 68, 222
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 17, 998
BsaXI ACNNNNNCTCC 6 cut(s) 256, 286, 356, 386, 419, 449
Bsc4I CCNNNNNNNGG 2 cut(s) 444, 664
Bse118I RCCGGY 2 cut(s) 224, 288
Bse1I ACTGG 2 cut(s) 937, 1033
Bse3DI GCAATG 1 cut(s) 574
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 1000, 1306
BseCI ATCGAT 1 cut(s) 396
BseDI CCNNGG 2 cut(s) 17, 998
BseGI GGATG 2 cut(s) 13, 829
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 444, 664
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 2 cut(s) 911, 1370
BseNI ACTGG 2 cut(s) 937, 1033
BseRI GAGGAG 7 cut(s) 41, 101, 188, 406, 409, 412, 593
BseXI GCAGC 3 cut(s) 48, 713, 1337
BseYI CCCAGC 2 cut(s) 45, 649
Bsh1236I CGCG 3 cut(s) 177, 321, 1334
Bsh1285I CGRYCG 3 cut(s) 135, 175, 610
BshFI GGCC 3 cut(s) 384, 509, 614
BshNI GGYRCC 1 cut(s) 221
BshVI ATCGAT 1 cut(s) 396
BsiEI CGRYCG 3 cut(s) 135, 175, 610
BsiHKAI GWGCWC 1 cut(s) 1378
BsiSI CCGG 5 cut(s) 225, 289, 351, 510, 658
BslFI GGGAC 3 cut(s) 160, 478, 674
BslI CCNNNNNNNGG 2 cut(s) 444, 664
BsmAI GTCTC 1 cut(s) 257
BsmFI GGGAC 3 cut(s) 160, 478, 674
BsmI GAATGC 1 cut(s) 1070
BsnI GGCC 3 cut(s) 384, 509, 614
Bso31I GGTCTC 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 1378
BspANI GGCC 3 cut(s) 384, 509, 614
BspCNI CTCAG 2 cut(s) 910, 1369
BspDI ATCGAT 1 cut(s) 396
BspFNI CGCG 3 cut(s) 177, 321, 1334
BspHI TCATGA 1 cut(s) 867
BspLI GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 829
BspMAI CTGCAG 1 cut(s) 703
BspOI GCTAGC 1 cut(s) 1332
BspPI GGATC 3 cut(s) 392, 401, 1225
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 257
BsrBI CCGCTC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 574
BsrFI RCCGGY 2 cut(s) 224, 288
BsrI ACTGG 2 cut(s) 937, 1033
BssAI RCCGGY 2 cut(s) 224, 288
BssECI CCNNGG 2 cut(s) 17, 998
BssNI GRCGYC 2 cut(s) 68, 222
Bst2UI CCWGG 2 cut(s) 1000, 1306
Bst4CI ACNGT 5 cut(s) 133, 299, 710, 772, 1282
Bst6I CTCTTC 2 cut(s) 383, 1112
BstACI GRCGYC 2 cut(s) 68, 222
BstC8I GCNNGC 6 cut(s) 79, 220, 226, 290, 370, 1330
BstDEI CTNAG 3 cut(s) 331, 897, 1356
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 2 cut(s) 13, 829
BstFNI CGCG 3 cut(s) 177, 321, 1334
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 3 cut(s) 224, 489, 1334
BstMAI GTCTC 1 cut(s) 257
BstMCI CGRYCG 3 cut(s) 135, 175, 610
BstMWI GCNNNNNNNGC 5 cut(s) 19, 552, 1088, 1097, 1331
BstNI CCWGG 2 cut(s) 1000, 1306
BstSCI CCNGG 4 cut(s) 509, 657, 998, 1304
BstSFI CTRYAG 2 cut(s) 699, 1101
BstUI CGCG 3 cut(s) 177, 321, 1334
BstV1I GCAGC 3 cut(s) 48, 713, 1337
BstV2I GAAGAC 1 cut(s) 91
BstX2I RGATCY 2 cut(s) 251, 1217
BstYI RGATCY 2 cut(s) 251, 1217
Bsu15I ATCGAT 1 cut(s) 396
BsuI GTATCC 2 cut(s) 1051, 1312
BsuRI GGCC 3 cut(s) 384, 509, 614
BsuTUI ATCGAT 1 cut(s) 396
BtgI CCRYGG 1 cut(s) 17
BtgZI GCGATG 2 cut(s) 369, 606
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 2 cut(s) 13, 829
Cac8I GCNNGC 6 cut(s) 79, 220, 226, 290, 370, 1330
CaiI CAGNNNCTG 1 cut(s) 904
CciI TCATGA 1 cut(s) 867
CfoI GCGC 3 cut(s) 224, 489, 1334
Cfr10I RCCGGY 2 cut(s) 224, 288
Cfr13I GGNCC 5 cut(s) 147, 383, 612, 828, 934
ClaI ATCGAT 1 cut(s) 396
CseI GACGC 2 cut(s) 310, 374
Csp6I GTAC 3 cut(s) 940, 1048, 1205
CviAII CATG 2 cut(s) 744, 868
CviQI GTAC 3 cut(s) 940, 1048, 1205
DdeI CTNAG 3 cut(s) 331, 897, 1356
DinI GGCGCC 1 cut(s) 223
Eam1104I CTCTTC 2 cut(s) 383, 1112
EarI CTCTTC 2 cut(s) 383, 1112
EciI GGCGGA 1 cut(s) 275
Eco31I GGTCTC 1 cut(s) 257
Eco47I GGWCC 3 cut(s) 147, 828, 934
EcoO109I RGGNCCY 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 998, 1304
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
FaeI CATG 2 cut(s) 747, 871
FaiI YATR 7 cut(s) 745, 869, 992, 1014, 1103, 1298, 1313
FaqI GGGAC 3 cut(s) 160, 478, 674
FatI CATG 2 cut(s) 743, 867
FauI CCCGC 2 cut(s) 212, 326
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 2 cut(s) 171, 1277
Fnu4HI GCNGC 4 cut(s) 37, 589, 702, 1326
FokI GGATG 1 cut(s) 836
Fsp4HI GCNGC 4 cut(s) 37, 589, 702, 1326
FspBI CTAG 6 cut(s) 468, 540, 722, 1329, 1350, 1379
GlaI GCGC 3 cut(s) 223, 488, 1333
GluI GCNGC 4 cut(s) 37, 589, 702, 1326
GsaI CCCAGC 2 cut(s) 49, 653
GsuI CTGGAG 2 cut(s) 240, 475
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 3 cut(s) 384, 509, 614
HapII CCGG 5 cut(s) 225, 289, 351, 510, 658
HgaI GACGC 2 cut(s) 310, 374
HhaI GCGC 3 cut(s) 224, 489, 1334
Hin1I GRCGYC 2 cut(s) 68, 222
Hin1II CATG 2 cut(s) 747, 871
Hin6I GCGC 3 cut(s) 222, 487, 1332
HinP1I GCGC 3 cut(s) 222, 487, 1332
HincII GTYRAC 2 cut(s) 172, 1278
HindII GTYRAC 2 cut(s) 172, 1278
HinfI GANTC 3 cut(s) 1003, 1109, 1199
HpaII CCGG 5 cut(s) 225, 289, 351, 510, 658
HphI GGTGA 6 cut(s) 82, 302, 307, 511, 892, 935
Hpy166II GTNNAC 3 cut(s) 172, 798, 1278
Hpy188I TCNGA 6 cut(s) 56, 326, 393, 478, 529, 786
Hpy188III TCNNGA 5 cut(s) 631, 831, 844, 868, 1343
Hpy8I GTNNAC 3 cut(s) 172, 798, 1278
HpyAV CCTTC 9 cut(s) 103, 133, 164, 175, 192, 279, 566, 1012, 1136
HpyCH4III ACNGT 5 cut(s) 133, 299, 710, 772, 1282
HpyCH4IV ACGT 5 cut(s) 68, 500, 663, 671, 737
HpyCH4V TGCA 3 cut(s) 625, 701, 1325
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 552, 1088, 1097, 1331
HpyF3I CTNAG 3 cut(s) 331, 897, 1356
HpySE526I ACGT 5 cut(s) 68, 500, 663, 671, 737
Hsp92I GRCGYC 2 cut(s) 68, 222
Hsp92II CATG 2 cut(s) 747, 871
HspAI GCGC 3 cut(s) 222, 487, 1332
KasI GGCGCC 1 cut(s) 221
KflI GGGWCCC 1 cut(s) 147
KroI GCCGGC 2 cut(s) 224, 288
KroNI GCCGGC 2 cut(s) 226, 290
Ksp22I TGATCA 1 cut(s) 633
LmnI GCTCC 6 cut(s) 54, 377, 494, 646, 1021, 1373
Lsp1109I GCAGC 3 cut(s) 48, 713, 1337
LweI GCATC 6 cut(s) 22, 849, 877, 966, 1078, 1336
MaeI CTAG 6 cut(s) 468, 540, 722, 1329, 1350, 1379
MaeII ACGT 5 cut(s) 68, 500, 663, 671, 737
MaeIII GTNAC 1 cut(s) 733
MbiI CCGCTC 1 cut(s) 243
MboII GAAGA 9 cut(s) 91, 109, 299, 337, 370, 608, 670, 1129, 1263
MflI RGATCY 2 cut(s) 251, 1217
MhlI GDGCHC 1 cut(s) 1378
MluCI AATT 7 cut(s) 440, 515, 626, 717, 927, 1131, 1242
Mly113I GGCGCC 1 cut(s) 222
MmeI TCCRAC 3 cut(s) 79, 304, 1035
MreI CGCCGGCG 1 cut(s) 288
MroNI GCCGGC 2 cut(s) 224, 288
MseI TTAA 4 cut(s) 450, 968, 1154, 1235
MslI CAYNNNNRTG 1 cut(s) 866
MspI CCGG 5 cut(s) 225, 289, 351, 510, 658
MspR9I CCNGG 4 cut(s) 511, 659, 1000, 1306
Mva1269I GAATGC 1 cut(s) 1070
MvaI CCWGG 2 cut(s) 1000, 1306
MvnI CGCG 3 cut(s) 177, 321, 1334
MwoI GCNNNNNNNGC 5 cut(s) 19, 552, 1088, 1097, 1331
NaeI GCCGGC 2 cut(s) 226, 290
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 2 cut(s) 511, 659
NgoMIV GCCGGC 2 cut(s) 224, 288
NheI GCTAGC 1 cut(s) 1328
NlaIII CATG 2 cut(s) 747, 871
NlaIV GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 829
NmeAIII GCCGAG 1 cut(s) 286
PagI TCATGA 1 cut(s) 867
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 612
PctI GAATGC 1 cut(s) 1070
PdiI GCCGGC 2 cut(s) 226, 290
PfeI GAWTC 3 cut(s) 1003, 1109, 1199
PflFI GACNNNGTC 1 cut(s) 297
PkrI GCNGC 4 cut(s) 38, 590, 703, 1327
Ple19I CGATCG 1 cut(s) 610
PluTI GGCGCC 1 cut(s) 225
PpuMI RGGWCCY 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 147
Psp6I CCWGG 2 cut(s) 998, 1304
PspFI CCCAGC 2 cut(s) 45, 649
PspGI CCWGG 2 cut(s) 998, 1304
PspN4I GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 829
PspPI GGNCC 5 cut(s) 147, 383, 612, 828, 934
PspPPI RGGWCCY 1 cut(s) 147
PstI CTGCAG 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 904
PsuI RGATCY 2 cut(s) 251, 1217
PsyI GACNNNGTC 1 cut(s) 297
PvuI CGATCG 1 cut(s) 610
RsaI GTAC 3 cut(s) 941, 1049, 1206
RsaNI GTAC 3 cut(s) 940, 1048, 1205
RseI CAYNNNNRTG 1 cut(s) 866
SalI GTCGAC 2 cut(s) 170, 1276
SaqAI TTAA 4 cut(s) 450, 968, 1154, 1235
SatI GCNGC 4 cut(s) 37, 589, 702, 1326
Sau96I GGNCC 5 cut(s) 147, 383, 612, 828, 934
ScaI AGTACT 1 cut(s) 941
ScrFI CCNGG 4 cut(s) 511, 659, 1000, 1306
SduI GDGCHC 1 cut(s) 1378
SfaNI GCATC 6 cut(s) 22, 849, 877, 966, 1078, 1336
SfcI CTRYAG 2 cut(s) 699, 1101
SfoI GGCGCC 1 cut(s) 223
SgrAI CRCCGGYG 1 cut(s) 288
SinI GGWCC 3 cut(s) 147, 828, 934
SmiMI CAYNNNNRTG 1 cut(s) 866
Sse9I AATT 7 cut(s) 440, 515, 626, 717, 927, 1131, 1242
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 6 cut(s) 468, 540, 722, 1329, 1350, 1379
StyD4I CCNGG 4 cut(s) 509, 657, 998, 1304
TaaI ACNGT 5 cut(s) 133, 299, 710, 772, 1282
TaiI ACGT 5 cut(s) 71, 503, 666, 674, 740
TasI AATT 7 cut(s) 440, 515, 626, 717, 927, 1131, 1242
TatI WGTACW 2 cut(s) 939, 1204
TauI GCSGC 1 cut(s) 591
TfiI GAWTC 3 cut(s) 1003, 1109, 1199
Tru1I TTAA 4 cut(s) 450, 968, 1154, 1235
Tru9I TTAA 4 cut(s) 450, 968, 1154, 1235
TseI GCWGC 3 cut(s) 36, 701, 1325
TspDTI ATGAA 4 cut(s) 585, 609, 732, 850
TspGWI ACGGA 2 cut(s) 573, 654
Tth111I GACNNNGTC 1 cut(s) 297
VpaK11BI GGWCC 3 cut(s) 147, 828, 934
XapI RAATTY 2 cut(s) 515, 1242
XmiI GTMKAC 2 cut(s) 171, 1277
XspI CTAG 6 cut(s) 468, 540, 722, 1329, 1350, 1379
ZraI GACGTC 1 cut(s) 69
ZrmI AGTACT 1 cut(s) 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.