MD12G1115900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011460
Physical Location & Seq
Reverse (-)
18459361 .. 18461113
1753 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1115900.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGAGCGATCAGCATCCACGGTTGAACCACCTCCGCAGCACCTCCCAGCTCCTCCGACAAGCCACGACGTCGTTCGCCGGCAACCTCTTCACCTTCGTCTTCCTCTCCCTCCTCATCTTCTCCTTCCGTACGGTCATCGAAAATGGGACCCACCTCCTCACATCCTTCGTCGACCGCGACCCTTCTCTCAAATCCCTCCTCTCCCGCCTCGATCTCGCTGGCGCCGGAAATCCCAACCACCGGTCTCCCCGATCTCCGGCCGAGTCCCCTTCTCCAATCACTCTCCGCCGCCGACACCGCCCATTTCTCCACCTCACCCGGGTCGGAACCTTAGACGAGGATTTCTTCTCCGGTGATGACGACGACGCCCGCTCCCTCTTCGGCCCCAATCGGATCGCCCCCATCCTCATTTTCTCCTCCTCCTCTCACTCCACCTCCAAATTAGGGTTTAATGGAACTTACGGGACTAGGGTTTCTGAAATTGTGCGCTCCGGCCTCACGTTCAAGGCCGAGAAATTCACCATTTCCGATGATAGTGCTAGAGACAGAGATAGCGACGGAGATAGCAAGGACCGAGAAGAGAAAGGCGGCGATGAAGACATGGACAATCGGGCTGTCGATTTGCAATTCTTCATCAAGGGATTGGAGCTAGGTCGCCAGGACGTGGCGACGTTGTTCTTCCTTGTGAGTTTCTTATCCGCAGCTTACGGTTGGCTATTTATCAGGTTAAAGTTGATGCCTTTTTCGGTTATGCCTCCCTGGATTCGAGGTTATGAGAAGGAGATTACTGGGTTTTTGTTTATGTGGTACTTCTTGGATACATTGGTGGCATTCGTATTTTCTGTCGATGCATGGGTTGCCATAGTGGATTCAAGGAAGAGTGGGAGAGAGATTGTCAAGGAAGGCTGTCATTTAATGTTGTCAATGTTGAGCCAAGCTATCCAAATTAAGTGTTTAGAATCTATATTTTGTGGGGGATCTGTAAGATGGGCTTTAACTCGAATTTGTGGAAGATCGTTTGCCAAGCTCTTTCAGTCGACTGTGGAAGTTTACTTTATGGTGGCTTGGCTCATATTTTACTTTGCAGCGAGGGCGAGGAGTAGAGATGCTAGTTCTCAGGGGGAGAGGATTGGAGCCCTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

382

Amino Acids

42.99

Weight (kDa)

8.64

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7813 PF25105 10 - 239 3.5e-66 Family of unknown function (DUF7813)
DUF7813 PF25105 239 - 379 4.5e-70 Family of unknown function (DUF7813)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37035
fragaria_vesca FvH4_6g16100
malus_domestica MD04G1095900.v1.1 MD12G1115900.v1.1
prunus_persica Prupe.6G235000_v2.0.a1
pyrus_communis pycom04g09080 pycom12g11540 pycom15g07040
rosa_chinensis RchiOBHm_Chr3g0469941
rosa_laevigata RLG00000024311
rosa_multiflora Rmu_sc0008347.1_g000001
rosa_roxburghii Rroxscaffold_5G00354980 Rroxscaffold_6G00411350
rosa_rugosa Rorug03G0105600 Rorug05G0154500
rosa_samantha Rh3AG154500 Rh3BG178600 Rh3CG169200 Rh3DG196500
rosa_wichuraiana Rw3G014560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 221
AccB7I CCANNNNNTGG 1 cut(s) 664
AccBSI CCGCTC 1 cut(s) 372
AccI GTMKAC 2 cut(s) 171, 1037
AccII CGCG 1 cut(s) 177
AciI CCGC 9 cut(s) 34, 175, 205, 286, 289, 298, 370, 588, 699
AclWI GGATC 2 cut(s) 401, 985
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 2 cut(s) 515, 1002
AcyI GRCGYC 3 cut(s) 68, 222, 366
AfaI GTAC 2 cut(s) 130, 809
AfiI CCNNNNNNNGG 5 cut(s) 240, 256, 319, 444, 664
AgeI ACCGGT 1 cut(s) 240
AgsI TTSAA 3 cut(s) 25, 505, 873
AjiI CACGTC 1 cut(s) 664
AjnI CCWGG 2 cut(s) 657, 758
AluBI AGCT 5 cut(s) 49, 649, 704, 938, 1027
AluI AGCT 5 cut(s) 49, 649, 704, 938, 1027
Alw26I GTCTC 2 cut(s) 249, 537
AlwI GGATC 2 cut(s) 401, 985
Ama87I CYCGRG 1 cut(s) 318
AoxI GGCC 4 cut(s) 258, 382, 493, 507
ApeKI GCWGC 3 cut(s) 36, 701, 1085
ApoI RAATTY 2 cut(s) 515, 1002
AsiGI ACCGGT 1 cut(s) 240
AspLEI GCGC 2 cut(s) 224, 489
AspS9I GGNCC 3 cut(s) 147, 383, 571
AsuC2I CCSGG 2 cut(s) 319, 320
AsuHPI GGTGA 4 cut(s) 82, 307, 365, 511
AvaI CYCGRG 1 cut(s) 318
AvaII GGWCC 2 cut(s) 147, 571
BaeI ACNNNNGTAYC 2 cut(s) 810, 843
BanI GGYRCC 1 cut(s) 221
BanII GRGCYC 1 cut(s) 1138
BbsI GAAGAC 2 cut(s) 91, 603
BbvI GCAGC 3 cut(s) 48, 713, 1097
BccI CCATC 2 cut(s) 410, 981
BcgI CGANNNNNNTGC 2 cut(s) 518, 552
BciT130I CCWGG 2 cut(s) 659, 760
BciVI GTATCC 1 cut(s) 811
BcnI CCSGG 2 cut(s) 319, 320
BcoDI GTCTC 2 cut(s) 249, 537
BfaI CTAG 5 cut(s) 468, 540, 650, 1110, 1139
BfoI RGCGCY 1 cut(s) 225
BfuI GTATCC 1 cut(s) 811
BisI GCNGC 5 cut(s) 37, 289, 589, 702, 1086
BlsI GCNGC 5 cut(s) 38, 290, 590, 703, 1087
Bme1390I CCNGG 4 cut(s) 319, 320, 659, 760
Bme18I GGWCC 2 cut(s) 147, 571
BmeT110I CYCGRG 1 cut(s) 318
BmgBI CACGTC 1 cut(s) 664
BmgT120I GGNCC 3 cut(s) 147, 383, 571
BmiI GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 1135
BmrFI CCNGG 4 cut(s) 319, 320, 659, 760
BmrI ACTGGG 1 cut(s) 798
BmsI GCATC 4 cut(s) 22, 726, 838, 1096
BmuI ACTGGG 1 cut(s) 798
BpiI GAAGAC 2 cut(s) 91, 603
BpuMI CCSGG 2 cut(s) 319, 320
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 3 cut(s) 68, 222, 366
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 3 cut(s) 17, 318, 758
BsaWI WCCGGW 2 cut(s) 240, 350
BsaXI ACNNNNNCTCC 4 cut(s) 356, 386, 419, 449
Bsc4I CCNNNNNNNGG 5 cut(s) 240, 256, 319, 444, 664
Bse118I RCCGGY 2 cut(s) 77, 240
Bse1I ACTGG 1 cut(s) 793
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 659, 760
BseDI CCNNGG 3 cut(s) 17, 318, 758
BseGI GGATG 3 cut(s) 13, 161, 402
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 5 cut(s) 240, 256, 319, 444, 664
BseMII CTCAG 1 cut(s) 1130
BseNI ACTGG 1 cut(s) 793
BseRI GAGGAG 8 cut(s) 41, 101, 146, 188, 406, 409, 412, 1111
BseX3I CGGCCG 1 cut(s) 258
BseXI GCAGC 3 cut(s) 48, 713, 1097
BseYI CCCAGC 1 cut(s) 45
Bsh1236I CGCG 1 cut(s) 177
Bsh1285I CGRYCG 2 cut(s) 175, 261
BshFI GGCC 4 cut(s) 260, 384, 495, 509
BshNI GGYRCC 1 cut(s) 221
BshTI ACCGGT 1 cut(s) 240
BsiEI CGRYCG 2 cut(s) 175, 261
BsiHKCI CYCGRG 1 cut(s) 318
BsiSI CCGG 7 cut(s) 78, 225, 241, 257, 319, 351, 492
BsiWI CGTACG 1 cut(s) 128
BslFI GGGAC 3 cut(s) 160, 250, 478
BslI CCNNNNNNNGG 5 cut(s) 240, 256, 319, 444, 664
BsmAI GTCTC 2 cut(s) 249, 537
BsmFI GGGAC 3 cut(s) 160, 250, 478
BsmI GAATGC 1 cut(s) 830
BsnI GGCC 4 cut(s) 260, 384, 495, 509
Bso31I GGTCTC 1 cut(s) 249
BsoBI CYCGRG 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 1138
Bsp143I GATC 6 cut(s) 7, 211, 251, 393, 977, 1013
BspACI CCGC 9 cut(s) 34, 175, 205, 286, 289, 298, 370, 588, 699
BspANI GGCC 4 cut(s) 260, 384, 495, 509
BspCNI CTCAG 1 cut(s) 1129
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 1135
BspPI GGATC 2 cut(s) 401, 985
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 249
BsrBI CCGCTC 1 cut(s) 372
BsrFI RCCGGY 2 cut(s) 77, 240
BsrI ACTGG 1 cut(s) 793
BssAI RCCGGY 2 cut(s) 77, 240
BssECI CCNNGG 3 cut(s) 17, 318, 758
BssMI GATC 6 cut(s) 7, 211, 251, 393, 977, 1013
BssNI GRCGYC 3 cut(s) 68, 222, 366
Bst2UI CCWGG 2 cut(s) 659, 760
Bst4CI ACNGT 4 cut(s) 21, 133, 710, 1042
Bst6I CTCTTC 4 cut(s) 92, 383, 573, 872
BstACI GRCGYC 3 cut(s) 68, 222, 366
BstAPI GCANNNNNTGC 1 cut(s) 857
BstC8I GCNNGC 3 cut(s) 79, 220, 370
BstDEI CTNAG 2 cut(s) 331, 1116
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 3 cut(s) 13, 161, 402
BstFNI CGCG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 2 cut(s) 224, 489
BstKTI GATC 6 cut(s) 10, 214, 254, 396, 980, 1016
BstMAI GTCTC 2 cut(s) 249, 537
BstMBI GATC 6 cut(s) 7, 211, 251, 393, 977, 1013
BstMCI CGRYCG 2 cut(s) 175, 261
BstMWI GCNNNNNNNGC 3 cut(s) 297, 857, 1091
BstNI CCWGG 2 cut(s) 659, 760
BstSCI CCNGG 4 cut(s) 317, 318, 657, 758
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 3 cut(s) 48, 713, 1097
BstV2I GAAGAC 2 cut(s) 91, 603
BstX2I RGATCY 1 cut(s) 977
BstYI RGATCY 1 cut(s) 977
BstZI CGGCCG 1 cut(s) 258
BsuI GTATCC 1 cut(s) 811
BsuRI GGCC 4 cut(s) 260, 384, 495, 509
BtgI CCRYGG 1 cut(s) 17
BtgZI GCGATG 1 cut(s) 606
BtrI CACGTC 1 cut(s) 664
BtsCI GGATG 3 cut(s) 13, 161, 402
Cac8I GCNNGC 3 cut(s) 79, 220, 370
CfoI GCGC 2 cut(s) 224, 489
Cfr10I RCCGGY 2 cut(s) 77, 240
Cfr13I GGNCC 3 cut(s) 147, 383, 571
Cfr9I CCCGGG 1 cut(s) 318
CseI GACGC 1 cut(s) 374
Csp6I GTAC 2 cut(s) 129, 808
CspAI ACCGGT 1 cut(s) 240
CviAII CATG 2 cut(s) 601, 852
CviQI GTAC 2 cut(s) 129, 808
DdeI CTNAG 2 cut(s) 331, 1116
DinI GGCGCC 1 cut(s) 223
DpnI GATC 6 cut(s) 9, 213, 253, 395, 979, 1015
DpnII GATC 6 cut(s) 7, 211, 251, 393, 977, 1013
EaeI YGGCCR 1 cut(s) 258
EagI CGGCCG 1 cut(s) 258
Eam1104I CTCTTC 4 cut(s) 92, 383, 573, 872
EarI CTCTTC 4 cut(s) 92, 383, 573, 872
EciI GGCGGA 1 cut(s) 275
EclXI CGGCCG 1 cut(s) 258
Eco24I GRGCYC 1 cut(s) 1138
Eco31I GGTCTC 1 cut(s) 249
Eco47I GGWCC 2 cut(s) 147, 571
Eco52I CGGCCG 1 cut(s) 258
Eco88I CYCGRG 1 cut(s) 318
EcoO109I RGGNCCY 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 657, 758
EcoT22I ATGCAT 1 cut(s) 853
EcoT38I GRGCYC 1 cut(s) 1138
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
FaeI CATG 2 cut(s) 604, 855
FaiI YATR 9 cut(s) 602, 752, 774, 803, 853, 863, 965, 1058, 1073
FalI AAGNNNNNCTT 2 cut(s) 976, 1008
FaqI GGGAC 3 cut(s) 160, 250, 478
FatI CATG 2 cut(s) 600, 851
FauI CCCGC 2 cut(s) 212, 377
FblI GTMKAC 2 cut(s) 171, 1037
Fnu4HI GCNGC 5 cut(s) 37, 289, 589, 702, 1086
FokI GGATG 2 cut(s) 148, 389
FriOI GRGCYC 1 cut(s) 1138
Fsp4HI GCNGC 5 cut(s) 37, 289, 589, 702, 1086
FspBI CTAG 5 cut(s) 468, 540, 650, 1110, 1139
GlaI GCGC 2 cut(s) 223, 488
GluI GCNGC 5 cut(s) 37, 289, 589, 702, 1086
GsaI CCCAGC 1 cut(s) 49
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 4 cut(s) 260, 384, 495, 509
HapII CCGG 7 cut(s) 78, 225, 241, 257, 319, 351, 492
HgaI GACGC 1 cut(s) 374
HhaI GCGC 2 cut(s) 224, 489
Hin1I GRCGYC 3 cut(s) 68, 222, 366
Hin1II CATG 2 cut(s) 604, 855
Hin6I GCGC 2 cut(s) 222, 487
HinP1I GCGC 2 cut(s) 222, 487
HincII GTYRAC 2 cut(s) 172, 1038
HindII GTYRAC 2 cut(s) 172, 1038
HinfI GANTC 4 cut(s) 263, 763, 869, 959
HpaII CCGG 7 cut(s) 78, 225, 241, 257, 319, 351, 492
HphI GGTGA 4 cut(s) 82, 307, 365, 511
Hpy166II GTNNAC 3 cut(s) 172, 1038, 1051
Hpy188I TCNGA 5 cut(s) 56, 326, 393, 478, 529
Hpy8I GTNNAC 3 cut(s) 172, 1038, 1051
Hpy99I CGWCG 7 cut(s) 70, 73, 173, 365, 368, 560, 673
HpyAV CCTTC 7 cut(s) 103, 133, 175, 192, 279, 772, 896
HpyCH4III ACNGT 4 cut(s) 21, 133, 710, 1042
HpyCH4IV ACGT 4 cut(s) 68, 500, 663, 671
HpyCH4V TGCA 3 cut(s) 625, 851, 1085
HpyF10VI GCNNNNNNNGC 3 cut(s) 297, 857, 1091
HpyF3I CTNAG 2 cut(s) 331, 1116
HpySE526I ACGT 4 cut(s) 68, 500, 663, 671
Hsp92I GRCGYC 3 cut(s) 68, 222, 366
Hsp92II CATG 2 cut(s) 604, 855
HspAI GCGC 2 cut(s) 222, 487
KasI GGCGCC 1 cut(s) 221
KflI GGGWCCC 1 cut(s) 147
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Kzo9I GATC 6 cut(s) 7, 211, 251, 393, 977, 1013
LmnI GCTCC 5 cut(s) 54, 377, 494, 646, 1133
Lsp1109I GCAGC 3 cut(s) 48, 713, 1097
LweI GCATC 4 cut(s) 22, 726, 838, 1096
MaeI CTAG 5 cut(s) 468, 540, 650, 1110, 1139
MaeII ACGT 4 cut(s) 68, 500, 663, 671
MalI GATC 6 cut(s) 9, 213, 253, 395, 979, 1015
MbiI CCGCTC 1 cut(s) 372
MboI GATC 6 cut(s) 7, 211, 251, 393, 977, 1013
MflI RGATCY 1 cut(s) 977
MhlI GDGCHC 1 cut(s) 1138
MluCI AATT 6 cut(s) 440, 480, 515, 626, 945, 1002
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 2 cut(s) 79, 304
Mph1103I ATGCAT 1 cut(s) 853
MroNI GCCGGC 1 cut(s) 77
MseI TTAA 5 cut(s) 450, 728, 914, 948, 995
MspI CCGG 7 cut(s) 78, 225, 241, 257, 319, 351, 492
MspR9I CCNGG 4 cut(s) 319, 320, 659, 760
Mva1269I GAATGC 1 cut(s) 830
MvaI CCWGG 2 cut(s) 659, 760
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 3 cut(s) 297, 857, 1091
NaeI GCCGGC 1 cut(s) 79
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 2 cut(s) 319, 320
NdeII GATC 6 cut(s) 7, 211, 251, 393, 977, 1013
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 2 cut(s) 604, 855
NlaIV GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 1135
NmeAIII GCCGAG 2 cut(s) 286, 535
NsiI ATGCAT 1 cut(s) 853
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 1 cut(s) 830
PdiI GCCGGC 1 cut(s) 79
PfeI GAWTC 3 cut(s) 763, 869, 959
Pfl23II CGTACG 1 cut(s) 128
PflMI CCANNNNNTGG 1 cut(s) 664
PinAI ACCGGT 1 cut(s) 240
PkrI GCNGC 5 cut(s) 38, 290, 590, 703, 1087
PleI GAGTC 1 cut(s) 271
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 147
Psp6I CCWGG 2 cut(s) 657, 758
PspFI CCCAGC 1 cut(s) 45
PspGI CCWGG 2 cut(s) 657, 758
PspLI CGTACG 1 cut(s) 128
PspN4I GGNNCC 6 cut(s) 148, 149, 223, 328, 385, 1135
PspPI GGNCC 3 cut(s) 147, 383, 571
PspPPI RGGWCCY 1 cut(s) 147
PsuI RGATCY 1 cut(s) 977
RsaI GTAC 2 cut(s) 130, 809
RsaNI GTAC 2 cut(s) 129, 808
SalI GTCGAC 2 cut(s) 170, 1036
SaqAI TTAA 5 cut(s) 450, 728, 914, 948, 995
SatI GCNGC 5 cut(s) 37, 289, 589, 702, 1086
Sau3AI GATC 6 cut(s) 7, 211, 251, 393, 977, 1013
Sau96I GGNCC 3 cut(s) 147, 383, 571
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 319, 320, 659, 760
SduI GDGCHC 1 cut(s) 1138
SfaNI GCATC 4 cut(s) 22, 726, 838, 1096
SfoI GGCGCC 1 cut(s) 223
SinI GGWCC 2 cut(s) 147, 571
SmaI CCCGGG 1 cut(s) 320
Sse9I AATT 6 cut(s) 440, 480, 515, 626, 945, 1002
SsiI CCGC 9 cut(s) 34, 175, 205, 286, 289, 298, 370, 588, 699
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 5 cut(s) 468, 540, 650, 1110, 1139
StyD4I CCNGG 4 cut(s) 317, 318, 657, 758
TaaI ACNGT 4 cut(s) 21, 133, 710, 1042
TaiI ACGT 4 cut(s) 71, 503, 666, 674
TaqI TCGA 8 cut(s) 138, 171, 210, 618, 766, 846, 1000, 1037
TaqII GACCGA 1 cut(s) 588
TasI AATT 6 cut(s) 440, 480, 515, 626, 945, 1002
TauI GCSGC 2 cut(s) 291, 591
TfiI GAWTC 3 cut(s) 763, 869, 959
Tru1I TTAA 5 cut(s) 450, 728, 914, 948, 995
Tru9I TTAA 5 cut(s) 450, 728, 914, 948, 995
TseI GCWGC 3 cut(s) 36, 701, 1085
TspDTI ATGAA 2 cut(s) 609, 622
TspGWI ACGGA 2 cut(s) 116, 573
TspMI CCCGGG 1 cut(s) 318
Van91I CCANNNNNTGG 1 cut(s) 664
VpaK11BI GGWCC 2 cut(s) 147, 571
XapI RAATTY 2 cut(s) 515, 1002
XmaI CCCGGG 1 cut(s) 318
XmiI GTMKAC 2 cut(s) 171, 1037
XspI CTAG 5 cut(s) 468, 540, 650, 1110, 1139
ZraI GACGTC 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.