Rroxscaffold_6G00411350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011460
Physical Location & Seq
Reverse (-)
33856674 .. 33858024
1351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00411350.1

Sequence Viewer

Length: 1272 bp
ATGAGCGATCAGCACCACCACTACCACCCGCGTCTCCACCACCTCCGGAGCACGCCACAGCTCCTCCGGCAAGCCTCCGCCTCCTTCTTCTCCAACCTCCTCACCTTCCTCTTCCTCTCCCTCCTCATCTTCTCCTTCCGCACCGTCGTCGAGAACGGGACCCACCTCCTCACCGCCTTCATCGACCGCGACCCTTCCCTCAAGTCCCTCCTCTCCCGCCTCGACCTCGCCGCCAACCCCACCTCCTCCCGATCCCCCCAGCCCTCTCCGGTCACCCTCCGCCGCCGCCACCGCCCCTTCCTCCACCTCACCCGCGTCGGAACCCTCGACGAGGACTTCTTCTCCGGCGAGGACGACGACGACTCCCGCGCCCTCTTCGGCTCCACCCGCAAAGCCCCAATCTTCATCTTCTCTCACTTCACCTCCAAATTAGGGAAAGCGACACACAGAGACGGCGGCGATAATGAGGAGGAGGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAGAAAGGAAATTCAGAAAATTCAGAAATGGATCGGATTGTGGATTTGCAATTCTTCATCAAAGGATTGGAGCTGGGTCAACGCGACTTGGCGGCCTTGTTCTTCCTTGTGAGTTTCTTATCTGCTGCATATGGCTGGGTTATTCTAGGGTTTCTTGTTACTTATTCTTGGGTTCTCGGCATTGTGTTCACCACAGTTGTTAATGATCTGATAGGGAGGTTTAATTCGTTTATTGGTTTAGTTTGGGATGGGTCTAGATTGGGAGTCAAGAGATTGTCTGGCTTCATTTTGATGAGGTGGGCTGTGAGAGACGCACTCACTCAGCTTCTAGGATTGTGGTATTTCGGTGAGATTGAGGACCAGTACTCTTTTTTTAAGCTCTTCATTAGGTTAAAGTTGATGCCTTTCTCTGTTATGCCTCCCTGGATTCGAGGTTTCGAGAAGGAGATTTCCGGGTTTCTGTTGATGTGGATATTTGTGGATACGATTGTGGCGTTCGTGTTTGCCGTGGATGCTTGGATTGCGATAGTGGATTCTAGGAAGAGTGGGAAGGAGATTGTTAAGGAGGGGTTGCAGTTGGTAGGATCGATGTTCAGCCAAGCTCTGCAAATTAAGTGCTTGGAACTTATATTTTGTGGGACGTCTGTAAAATGGGGTCTGACTCGTGTTTTTGGAAGATCGTTTGCCAAGGTCTTTCAGTCGACCATGGAAGTCTACTTTTTTGGTGGCTTGGTTGATATTTTACTTTGCAGCAAGGGCGAGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

423

Amino Acids

48.31

Weight (kDa)

5.82

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7813 PF25105 14 - 409 3.1e-173 Family of unknown function (DUF7813)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37035
fragaria_vesca FvH4_6g16100
malus_domestica MD04G1095900.v1.1 MD12G1115900.v1.1
prunus_persica Prupe.6G235000_v2.0.a1
pyrus_communis pycom04g09080 pycom12g11540 pycom15g07040
rosa_chinensis RchiOBHm_Chr3g0469941
rosa_laevigata RLG00000024311
rosa_multiflora Rmu_sc0008347.1_g000001
rosa_roxburghii Rroxscaffold_5G00354980 Rroxscaffold_6G00411350
rosa_rugosa Rorug03G0105600 Rorug05G0154500
rosa_samantha Rh3AG154500 Rh3BG178600 Rh3CG169200 Rh3DG196500
rosa_wichuraiana Rw3G014560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1148
AccI GTMKAC 2 cut(s) 1205, 1218
AccII CGCG 5 cut(s) 31, 189, 315, 369, 588
AccIII TCCGGA 1 cut(s) 45
AclWI GGATC 3 cut(s) 246, 543, 1096
AcsI RAATTY 2 cut(s) 514, 523
AcyI GRCGYC 1 cut(s) 1145
AfaI GTAC 1 cut(s) 869
AfiI CCNNNNNNNGG 2 cut(s) 331, 432
AjnI CCWGG 1 cut(s) 926
AluBI AGCT 5 cut(s) 61, 577, 829, 883, 1106
AluI AGCT 5 cut(s) 61, 577, 829, 883, 1106
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 3 cut(s) 38, 444, 807
AlwI GGATC 3 cut(s) 246, 543, 1096
Aor13HI TCCGGA 1 cut(s) 45
AoxI GGCC 1 cut(s) 597
ApeKI GCWGC 2 cut(s) 629, 1254
ApoI RAATTY 2 cut(s) 514, 523
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 2 cut(s) 159, 862
AsuC2I CCSGG 1 cut(s) 958
AsuHPI GGTGA 7 cut(s) 94, 163, 265, 301, 412, 685, 863
AvaII GGWCC 2 cut(s) 159, 862
BaeI ACNNNNGTAYC 2 cut(s) 978, 1011
BauI CACGAG 1 cut(s) 1167
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 616, 1266
BccI CCATC 1 cut(s) 746
BceAI ACGGC 2 cut(s) 469, 995
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 1 cut(s) 928
BciVI GTATCC 1 cut(s) 979
BcnI CCSGG 1 cut(s) 958
BcoDI GTCTC 3 cut(s) 38, 444, 807
BfaI CTAG 4 cut(s) 650, 759, 833, 1041
BfuI GTATCC 1 cut(s) 979
BisI GCNGC 7 cut(s) 231, 283, 286, 457, 597, 630, 1255
BlsI GCNGC 7 cut(s) 232, 284, 287, 458, 598, 631, 1256
BmcAI AGTACT 1 cut(s) 869
Bme1390I CCNGG 2 cut(s) 928, 958
Bme18I GGWCC 2 cut(s) 159, 862
BmgT120I GGNCC 2 cut(s) 159, 862
BmiI GGNNCC 4 cut(s) 160, 161, 322, 382
BmrFI CCNGG 2 cut(s) 928, 958
BmsI GCATC 2 cut(s) 894, 1006
BplI GAGNNNNNCTC 2 cut(s) 804, 836
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 958
Bsa29I ATCGAT 1 cut(s) 1091
BsaHI GRCGYC 1 cut(s) 1145
BsaJI CCNNGG 4 cut(s) 926, 1011, 1191, 1209
BsaWI WCCGGW 2 cut(s) 45, 268
Bsc4I CCNNNNNNNGG 2 cut(s) 331, 432
Bse1I ACTGG 1 cut(s) 865
BseAI TCCGGA 1 cut(s) 45
BseBI CCWGG 1 cut(s) 928
BseCI ATCGAT 1 cut(s) 1091
BseDI CCNNGG 4 cut(s) 926, 1011, 1191, 1209
BseGI GGATG 2 cut(s) 757, 1021
BseLI CCNNNNNNNGG 2 cut(s) 331, 432
BseMII CTCAG 1 cut(s) 839
BseNI ACTGG 1 cut(s) 865
BseXI GCAGC 2 cut(s) 616, 1266
BseYI CCCAGC 3 cut(s) 258, 577, 639
Bsh1236I CGCG 5 cut(s) 31, 189, 315, 369, 588
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 599
BshVI ATCGAT 1 cut(s) 1091
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 5 cut(s) 46, 67, 269, 345, 957
BslFI GGGAC 3 cut(s) 172, 190, 1156
BslI CCNNNNNNNGG 2 cut(s) 331, 432
BsmAI GTCTC 3 cut(s) 38, 444, 807
BsmBI CGTCTC 3 cut(s) 38, 444, 807
BsmFI GGGAC 3 cut(s) 172, 190, 1156
BsnI GGCC 1 cut(s) 599
Bsp1286I GDGCHC 1 cut(s) 53
Bsp13I TCCGGA 1 cut(s) 45
Bsp143I GATC 6 cut(s) 7, 251, 535, 709, 1088, 1181
Bsp19I CCATGG 1 cut(s) 1209
BspANI GGCC 1 cut(s) 599
BspCNI CTCAG 1 cut(s) 838
BspDI ATCGAT 1 cut(s) 1091
BspEI TCCGGA 1 cut(s) 45
BspFNI CGCG 5 cut(s) 31, 189, 315, 369, 588
BspLI GGNNCC 4 cut(s) 160, 161, 322, 382
BspPI GGATC 3 cut(s) 246, 543, 1096
BspQI GCTCTTC 1 cut(s) 890
BsrI ACTGG 1 cut(s) 865
BssECI CCNNGG 4 cut(s) 926, 1011, 1191, 1209
BssMI GATC 6 cut(s) 7, 251, 535, 709, 1088, 1181
BssNI GRCGYC 1 cut(s) 1145
BssSI CACGAG 1 cut(s) 1167
BssT1I CCWWGG 2 cut(s) 1191, 1209
Bst2BI CACGAG 1 cut(s) 1167
Bst2UI CCWGG 1 cut(s) 928
Bst4CI ACNGT 2 cut(s) 145, 700
Bst6I CTCTTC 5 cut(s) 116, 380, 498, 890, 1040
BstACI GRCGYC 1 cut(s) 1145
BstC8I GCNNGC 2 cut(s) 53, 72
BstDEI CTNAG 1 cut(s) 825
BstDSI CCRYGG 2 cut(s) 1011, 1209
BstEII GGTNACC 1 cut(s) 271
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 2 cut(s) 757, 1021
BstFNI CGCG 5 cut(s) 31, 189, 315, 369, 588
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 6 cut(s) 10, 254, 538, 712, 1091, 1184
BstMAI GTCTC 3 cut(s) 38, 444, 807
BstMBI GATC 6 cut(s) 7, 251, 535, 709, 1088, 1181
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 6 cut(s) 67, 291, 387, 1016, 1025, 1260
BstNI CCWGG 1 cut(s) 928
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 2 cut(s) 926, 956
BstUI CGCG 5 cut(s) 31, 189, 315, 369, 588
BstV1I GCAGC 2 cut(s) 616, 1266
Bsu15I ATCGAT 1 cut(s) 1091
BsuI GTATCC 1 cut(s) 979
BsuRI GGCC 1 cut(s) 599
BsuTUI ATCGAT 1 cut(s) 1091
BtgI CCRYGG 2 cut(s) 1011, 1209
BtsCI GGATG 2 cut(s) 757, 1021
Cac8I GCNNGC 2 cut(s) 53, 72
CfoI GCGC 1 cut(s) 371
Cfr13I GGNCC 2 cut(s) 159, 862
ClaI ATCGAT 1 cut(s) 1091
CseI GACGC 3 cut(s) 20, 304, 824
Csp6I GTAC 1 cut(s) 868
CviAII CATG 1 cut(s) 1210
CviQI GTAC 1 cut(s) 868
DdeI CTNAG 1 cut(s) 825
DpnI GATC 6 cut(s) 9, 253, 537, 711, 1090, 1183
DpnII GATC 6 cut(s) 7, 251, 535, 709, 1088, 1181
Eam1104I CTCTTC 5 cut(s) 116, 380, 498, 890, 1040
EarI CTCTTC 5 cut(s) 116, 380, 498, 890, 1040
EciI GGCGGA 2 cut(s) 67, 269
Eco130I CCWWGG 2 cut(s) 1191, 1209
Eco47I GGWCC 2 cut(s) 159, 862
Eco91I GGTNACC 1 cut(s) 271
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 159
EcoO65I GGTNACC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 926
EcoT14I CCWWGG 2 cut(s) 1191, 1209
ErhI CCWWGG 2 cut(s) 1191, 1209
Esp3I CGTCTC 3 cut(s) 38, 444, 807
FaeI CATG 1 cut(s) 1213
FaiI YATR 5 cut(s) 634, 636, 920, 1133, 1211
FaqI GGGAC 3 cut(s) 172, 190, 1156
FatI CATG 1 cut(s) 1209
FauI CCCGC 5 cut(s) 36, 224, 320, 374, 395
FauNDI CATATG 1 cut(s) 634
FblI GTMKAC 2 cut(s) 1205, 1218
Fnu4HI GCNGC 7 cut(s) 231, 283, 286, 457, 597, 630, 1255
FokI GGATG 2 cut(s) 764, 1028
Fsp4HI GCNGC 7 cut(s) 231, 283, 286, 457, 597, 630, 1255
FspBI CTAG 4 cut(s) 650, 759, 833, 1041
GlaI GCGC 1 cut(s) 370
GluI GCNGC 7 cut(s) 231, 283, 286, 457, 597, 630, 1255
GsaI CCCAGC 3 cut(s) 262, 581, 643
HaeIII GGCC 1 cut(s) 599
HapII CCGG 5 cut(s) 46, 67, 269, 345, 957
HgaI GACGC 3 cut(s) 20, 304, 824
HhaI GCGC 1 cut(s) 371
Hin1I GRCGYC 1 cut(s) 1145
Hin1II CATG 1 cut(s) 1213
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HincII GTYRAC 2 cut(s) 584, 1206
HindII GTYRAC 2 cut(s) 584, 1206
HinfI GANTC 5 cut(s) 362, 768, 931, 1037, 1165
HpaII CCGG 5 cut(s) 46, 67, 269, 345, 957
HphI GGTGA 7 cut(s) 94, 163, 265, 301, 412, 685, 863
Hpy166II GTNNAC 4 cut(s) 584, 693, 1206, 1219
Hpy188I TCNGA 6 cut(s) 320, 520, 529, 540, 714, 1164
Hpy188III TCNNGA 6 cut(s) 46, 151, 249, 759, 772, 943
Hpy8I GTNNAC 4 cut(s) 584, 693, 1206, 1219
Hpy99I CGWCG 6 cut(s) 149, 152, 320, 332, 359, 362
HpyAV CCTTC 8 cut(s) 94, 115, 145, 187, 204, 307, 940, 1048
HpyCH4III ACNGT 2 cut(s) 145, 700
HpyCH4IV ACGT 1 cut(s) 1145
HpyCH4V TGCA 5 cut(s) 553, 632, 1078, 1111, 1254
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 291, 387, 1016, 1025, 1260
HpyF3I CTNAG 1 cut(s) 825
HpySE526I ACGT 1 cut(s) 1145
Hsp92I GRCGYC 1 cut(s) 1145
Hsp92II CATG 1 cut(s) 1213
HspAI GCGC 1 cut(s) 369
KflI GGGWCCC 1 cut(s) 159
Kpn2I TCCGGA 1 cut(s) 45
Kzo9I GATC 6 cut(s) 7, 251, 535, 709, 1088, 1181
LguI GCTCTTC 1 cut(s) 890
LmnI GCTCC 4 cut(s) 48, 66, 386, 574
Lsp1109I GCAGC 2 cut(s) 616, 1266
LweI GCATC 2 cut(s) 894, 1006
MaeI CTAG 4 cut(s) 650, 759, 833, 1041
MaeII ACGT 1 cut(s) 1145
MaeIII GTNAC 2 cut(s) 271, 661
MalI GATC 6 cut(s) 9, 253, 537, 711, 1090, 1183
MboI GATC 6 cut(s) 7, 251, 535, 709, 1088, 1181
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 6 cut(s) 428, 514, 523, 554, 727, 1113
MlyI GAGTC 3 cut(s) 356, 777, 1159
MmeI TCCRAC 2 cut(s) 117, 298
MroI TCCGGA 1 cut(s) 45
MseI TTAA 6 cut(s) 705, 726, 879, 896, 1065, 1116
MslI CAYNNNNRTG 1 cut(s) 794
MspI CCGG 5 cut(s) 46, 67, 269, 345, 957
MspR9I CCNGG 2 cut(s) 928, 958
MvaI CCWGG 1 cut(s) 928
MvnI CGCG 5 cut(s) 31, 189, 315, 369, 588
MwoI GCNNNNNNNGC 6 cut(s) 67, 291, 387, 1016, 1025, 1260
NciI CCSGG 1 cut(s) 958
NcoI CCATGG 1 cut(s) 1209
NdeI CATATG 1 cut(s) 634
NdeII GATC 6 cut(s) 7, 251, 535, 709, 1088, 1181
NlaIII CATG 1 cut(s) 1213
NlaIV GGNNCC 4 cut(s) 160, 161, 322, 382
NmeAIII GCCGAG 1 cut(s) 660
NmuCI GTSAC 1 cut(s) 271
PciSI GCTCTTC 1 cut(s) 890
PcsI WCGNNNNNNNCGW 3 cut(s) 324, 995, 1008
PfeI GAWTC 2 cut(s) 931, 1037
PkrI GCNGC 7 cut(s) 232, 284, 287, 458, 598, 631, 1256
PleI GAGTC 3 cut(s) 356, 776, 1159
PpsI GAGTC 3 cut(s) 356, 776, 1159
PpuMI RGGWCCY 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 926
PspEI GGTNACC 1 cut(s) 271
PspFI CCCAGC 3 cut(s) 258, 577, 639
PspGI CCWGG 1 cut(s) 926
PspN4I GGNNCC 4 cut(s) 160, 161, 322, 382
PspPI GGNCC 2 cut(s) 159, 862
PspPPI RGGWCCY 1 cut(s) 159
RsaI GTAC 1 cut(s) 869
RsaNI GTAC 1 cut(s) 868
RseI CAYNNNNRTG 1 cut(s) 794
SalI GTCGAC 1 cut(s) 1204
SapI GCTCTTC 1 cut(s) 890
SaqAI TTAA 6 cut(s) 705, 726, 879, 896, 1065, 1116
SatI GCNGC 7 cut(s) 231, 283, 286, 457, 597, 630, 1255
Sau3AI GATC 6 cut(s) 7, 251, 535, 709, 1088, 1181
Sau96I GGNCC 2 cut(s) 159, 862
ScaI AGTACT 1 cut(s) 869
SchI GAGTC 3 cut(s) 356, 777, 1159
ScrFI CCNGG 2 cut(s) 928, 958
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 2 cut(s) 894, 1006
SinI GGWCC 2 cut(s) 159, 862
SmiMI CAYNNNNRTG 1 cut(s) 794
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 6 cut(s) 428, 514, 523, 554, 727, 1113
SspMI CTAG 4 cut(s) 650, 759, 833, 1041
StyD4I CCNGG 2 cut(s) 926, 956
StyI CCWWGG 2 cut(s) 1191, 1209
TaaI ACNGT 2 cut(s) 145, 700
TaiI ACGT 1 cut(s) 1148
TaqI TCGA 8 cut(s) 150, 183, 222, 327, 934, 942, 1091, 1205
TasI AATT 6 cut(s) 428, 514, 523, 554, 727, 1113
TatI WGTACW 1 cut(s) 867
TauI GCSGC 5 cut(s) 233, 285, 288, 459, 599
TfiI GAWTC 2 cut(s) 931, 1037
Tru1I TTAA 6 cut(s) 705, 726, 879, 896, 1065, 1116
Tru9I TTAA 6 cut(s) 705, 726, 879, 896, 1065, 1116
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 2 cut(s) 629, 1254
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 5 cut(s) 169, 394, 550, 778, 877
VpaK11BI GGWCC 2 cut(s) 159, 862
XagI CCTNNNNNAGG 1 cut(s) 329
XapI RAATTY 2 cut(s) 514, 523
XbaI TCTAGA 1 cut(s) 758
XmiI GTMKAC 2 cut(s) 1205, 1218
XspI CTAG 4 cut(s) 650, 759, 833, 1041
ZraI GACGTC 1 cut(s) 1146
ZrmI AGTACT 1 cut(s) 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.