Rw3G014560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011460
Physical Location & Seq
Forward (+)
14805709 .. 14807096
1388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G014560.1

Sequence Viewer

Length: 1236 bp
ATGAGCGATCACCACCACCACCACCAGCACCACCCGCGGCTCCACCACCTCAGGAGCACGCCGCAGCTCCTCCGGCAAGCCTCGGCCTCCTTCTTCTCCAACCTCCTCACCTTCCTCTTCCTCTCCCTCCTCATCTTCTCCTTCCGCACCGTCGTCGAGAACGGGACCCACCTCCTCACCGCCTTCATCGACCGCGACCCTTCCCTCAAGTCCCTCCTCTCCCGCCTTGACCTCGCCGCCAACCCCACCTCCACCTCCTCCTCCCGATCCCCCCAGCCCTCTCCGGTCACCCTCCGCCGCCGCCACCGCCCCTTCCTCCAGCTCACCCGCGTCGGAACCCTCGACGAGGACTTCTTCTCCGGCGAAGACGACGACGACTCCCGCGCCCTCTTCGGCAAAGCCCCAATCTTCATCTTCTCTCACTTCACCTCCAAATTAGGGTTTAATGGGACTAGGGTTTCCGACATGGCCCGCTCCGGCCTCTCCTTCAAATCCGACCAATTCACCATCTCCAATGAGAAAGCGACACACAGAGACGGCGGCAATTCGGAAAATTCAGAAATGGATCGGATTGTGGATTTGCAATTCTTCATCAAGGGATTGGAGCTGGGTCAACGCGACGTGGCGGCCCTGTTCTTCCTTGTGAGTTTCTTATCTGCTGCATATGGCTGGGTCATTCTAGGGTTTCTTGTTACGTATTCTTGGGTTCTCGGCATTGTGTTCACCACGGTTGTTAATGATCTGATAGGGAGGTTTAATTCGTTTATTGGTTTAGTTTGGGATGGGTCTAGATTGGGAGTCAAGAGATTGTCCGGCTTCATTTTGATGAGGTGGGCTGTGAGAGACGCGCTCACTCAGCTTCTAGGATTGTGGTATTTCGGTGAGATTGAAGACCAGTACTCTTTTTTTAAGCTCTTCATTAGGTTAAAGTTGATGCCTTTCTCTGTTATGCCTCCCTGGATTCGGGGTTTCGAGAAGGAGATTTCCGGGTTTCTGTTGATGTGGATATTTGTGGATACGATTGTGGCGTTTGTCTTTGCCGTGGATGCTTGGATTGCGATAGTCGATTCTAGGAAGAGTGGGAAGGAGATTGTTAAGGAGGGATCGTTTGCCAAGGTCTTTCAGTCGACCATGGAAGTCTACTTTTTGGTGGCTTGGTTGATATTTTACTTTGCAGCAAGGGCGAGGTGTAGGGATGCTAGCTCACAGGGGGATAGAATTGGAGCACTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

411

Amino Acids

46.83

Weight (kDa)

9.29

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7813 PF25105 16 - 369 3.9e-156 Family of unknown function (DUF7813)
DUF7813 PF25105 369 - 408 1.2e-12 Family of unknown function (DUF7813)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37035
fragaria_vesca FvH4_6g16100
malus_domestica MD04G1095900.v1.1 MD12G1115900.v1.1
prunus_persica Prupe.6G235000_v2.0.a1
pyrus_communis pycom04g09080 pycom12g11540 pycom15g07040
rosa_chinensis RchiOBHm_Chr3g0469941
rosa_laevigata RLG00000024311
rosa_multiflora Rmu_sc0008347.1_g000001
rosa_roxburghii Rroxscaffold_5G00354980 Rroxscaffold_6G00411350
rosa_rugosa Rorug03G0105600 Rorug05G0154500
rosa_samantha Rh3AG154500 Rh3BG178600 Rh3CG169200 Rh3DG196500
rosa_wichuraiana Rw3G014560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 474
AccI GTMKAC 2 cut(s) 1127, 1140
AccII CGCG 6 cut(s) 37, 195, 330, 384, 618, 848
AclWI GGATC 3 cut(s) 261, 573, 1111
AcsI RAATTY 1 cut(s) 553
AfaI GTAC 1 cut(s) 899
AfiI CCNNNNNNNGG 3 cut(s) 346, 438, 963
AgsI TTSAA 2 cut(s) 490, 890
AjiI CACGTC 1 cut(s) 622
AjnI CCWGG 1 cut(s) 956
AluBI AGCT 6 cut(s) 67, 322, 607, 859, 913, 1203
AluI AGCT 6 cut(s) 67, 322, 607, 859, 913, 1203
Alw21I GWGCWC 2 cut(s) 59, 1228
Alw26I GTCTC 2 cut(s) 528, 837
AlwI GGATC 3 cut(s) 261, 573, 1111
AoxI GGCC 4 cut(s) 84, 468, 478, 627
ApeKI GCWGC 3 cut(s) 64, 659, 1175
ApoI RAATTY 1 cut(s) 553
AspLEI GCGC 2 cut(s) 386, 850
AspS9I GGNCC 3 cut(s) 165, 469, 628
AsuC2I CCSGG 1 cut(s) 988
AsuHPI GGTGA 8 cut(s) 100, 169, 280, 316, 418, 496, 715, 893
AsuNHI GCTAGC 1 cut(s) 1199
AvaII GGWCC 1 cut(s) 165
AxyI CCTNAGG 1 cut(s) 50
BaeI ACNNNNGTAYC 2 cut(s) 1008, 1041
BbsI GAAGAC 2 cut(s) 372, 897
Bbv12I GWGCWC 2 cut(s) 59, 1228
BbvI GCAGC 3 cut(s) 76, 646, 1187
BccI CCATC 2 cut(s) 515, 776
BceAI ACGGC 2 cut(s) 553, 1025
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 1 cut(s) 958
BciVI GTATCC 1 cut(s) 1009
BcnI CCSGG 1 cut(s) 988
BcoDI GTCTC 2 cut(s) 528, 837
BfaI CTAG 6 cut(s) 453, 680, 789, 863, 1071, 1200
BfuI GTATCC 1 cut(s) 1009
BmcAI AGTACT 1 cut(s) 899
Bme1390I CCNGG 2 cut(s) 958, 988
Bme18I GGWCC 1 cut(s) 165
BmgBI CACGTC 1 cut(s) 622
BmgT120I GGNCC 3 cut(s) 165, 469, 628
BmiI GGNNCC 4 cut(s) 41, 166, 167, 337
BmrFI CCNGG 2 cut(s) 958, 988
BmsI GCATC 3 cut(s) 924, 1036, 1186
BmtI GCTAGC 1 cut(s) 1203
BpiI GAAGAC 2 cut(s) 372, 897
BplI GAGNNNNNCTC 2 cut(s) 834, 866
BpmI CTGGAG 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 988
BsaAI YACGTR 1 cut(s) 696
BsaJI CCNNGG 7 cut(s) 35, 81, 726, 956, 1041, 1113, 1131
BsaWI WCCGGW 1 cut(s) 283
Bsc4I CCNNNNNNNGG 3 cut(s) 346, 438, 963
Bse1I ACTGG 1 cut(s) 895
Bse21I CCTNAGG 1 cut(s) 50
BseBI CCWGG 1 cut(s) 958
BseDI CCNNGG 7 cut(s) 35, 81, 726, 956, 1041, 1113, 1131
BseGI GGATG 3 cut(s) 787, 1051, 1201
BseLI CCNNNNNNNGG 3 cut(s) 346, 438, 963
BseMII CTCAG 2 cut(s) 64, 869
BseNI ACTGG 1 cut(s) 895
BseRI GAGGAG 7 cut(s) 59, 95, 119, 164, 206, 247, 250
BseXI GCAGC 3 cut(s) 76, 646, 1187
BseYI CCCAGC 3 cut(s) 273, 607, 669
Bsh1236I CGCG 6 cut(s) 37, 195, 330, 384, 618, 848
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 4 cut(s) 86, 470, 480, 629
BsiEI CGRYCG 1 cut(s) 193
BsiHKAI GWGCWC 2 cut(s) 59, 1228
BsiSI CCGG 6 cut(s) 73, 284, 360, 477, 813, 987
BslFI GGGAC 3 cut(s) 178, 196, 463
BslI CCNNNNNNNGG 3 cut(s) 346, 438, 963
BsmAI GTCTC 2 cut(s) 528, 837
BsmBI CGTCTC 2 cut(s) 528, 837
BsmFI GGGAC 3 cut(s) 178, 196, 463
BsnI GGCC 4 cut(s) 86, 470, 480, 629
Bsp1286I GDGCHC 2 cut(s) 59, 1228
Bsp143I GATC 5 cut(s) 7, 266, 565, 739, 1103
Bsp19I CCATGG 1 cut(s) 1131
BspANI GGCC 4 cut(s) 86, 470, 480, 629
BspCNI CTCAG 2 cut(s) 63, 868
BspFNI CGCG 6 cut(s) 37, 195, 330, 384, 618, 848
BspLI GGNNCC 4 cut(s) 41, 166, 167, 337
BspOI GCTAGC 1 cut(s) 1203
BspPI GGATC 3 cut(s) 261, 573, 1111
BspQI GCTCTTC 1 cut(s) 920
BsrBI CCGCTC 1 cut(s) 474
BsrI ACTGG 1 cut(s) 895
BssECI CCNNGG 7 cut(s) 35, 81, 726, 956, 1041, 1113, 1131
BssMI GATC 5 cut(s) 7, 266, 565, 739, 1103
BssT1I CCWWGG 2 cut(s) 1113, 1131
Bst2UI CCWGG 1 cut(s) 958
Bst4CI ACNGT 2 cut(s) 151, 730
Bst6I CTCTTC 4 cut(s) 122, 395, 920, 1070
BstBAI YACGTR 1 cut(s) 696
BstC8I GCNNGC 4 cut(s) 59, 78, 472, 1201
BstDEI CTNAG 2 cut(s) 50, 855
BstDSI CCRYGG 4 cut(s) 35, 726, 1041, 1131
BstEII GGTNACC 1 cut(s) 286
BstENI CCTNNNNNAGG 1 cut(s) 344
BstF5I GGATG 3 cut(s) 787, 1051, 1201
BstFNI CGCG 6 cut(s) 37, 195, 330, 384, 618, 848
BstHHI GCGC 2 cut(s) 386, 850
BstKTI GATC 5 cut(s) 10, 269, 568, 742, 1106
BstMAI GTCTC 2 cut(s) 528, 837
BstMBI GATC 5 cut(s) 7, 266, 565, 739, 1103
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 7 cut(s) 34, 73, 306, 856, 1046, 1055, 1181
BstNI CCWGG 1 cut(s) 958
BstPI GGTNACC 1 cut(s) 286
BstSCI CCNGG 2 cut(s) 956, 986
BstSNI TACGTA 1 cut(s) 696
BstUI CGCG 6 cut(s) 37, 195, 330, 384, 618, 848
BstV1I GCAGC 3 cut(s) 76, 646, 1187
BstV2I GAAGAC 2 cut(s) 372, 897
Bsu36I CCTNAGG 1 cut(s) 50
BsuI GTATCC 1 cut(s) 1009
BsuRI GGCC 4 cut(s) 86, 470, 480, 629
BtgI CCRYGG 4 cut(s) 35, 726, 1041, 1131
BtrI CACGTC 1 cut(s) 622
BtsCI GGATG 3 cut(s) 787, 1051, 1201
Cac8I GCNNGC 4 cut(s) 59, 78, 472, 1201
CfoI GCGC 2 cut(s) 386, 850
Cfr13I GGNCC 3 cut(s) 165, 469, 628
Cfr42I CCGCGG 1 cut(s) 38
CseI GACGC 2 cut(s) 319, 854
Csp6I GTAC 1 cut(s) 898
CviAII CATG 2 cut(s) 466, 1132
CviQI GTAC 1 cut(s) 898
DdeI CTNAG 2 cut(s) 50, 855
DpnI GATC 5 cut(s) 9, 268, 567, 741, 1105
DpnII GATC 5 cut(s) 7, 266, 565, 739, 1103
Eam1104I CTCTTC 4 cut(s) 122, 395, 920, 1070
EarI CTCTTC 4 cut(s) 122, 395, 920, 1070
EciI GGCGGA 1 cut(s) 284
Eco105I TACGTA 1 cut(s) 696
Eco130I CCWWGG 2 cut(s) 1113, 1131
Eco47I GGWCC 1 cut(s) 165
Eco81I CCTNAGG 1 cut(s) 50
Eco91I GGTNACC 1 cut(s) 286
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 165
EcoO65I GGTNACC 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 956
EcoT14I CCWWGG 2 cut(s) 1113, 1131
ErhI CCWWGG 2 cut(s) 1113, 1131
Esp3I CGTCTC 2 cut(s) 528, 837
FaeI CATG 2 cut(s) 469, 1135
FaiI YATR 5 cut(s) 467, 664, 666, 950, 1133
FaqI GGGAC 3 cut(s) 178, 196, 463
FatI CATG 2 cut(s) 465, 1131
FauI CCCGC 5 cut(s) 42, 230, 335, 389, 479
FauNDI CATATG 1 cut(s) 664
FblI GTMKAC 2 cut(s) 1127, 1140
FokI GGATG 3 cut(s) 794, 1058, 1208
FspBI CTAG 6 cut(s) 453, 680, 789, 863, 1071, 1200
GlaI GCGC 2 cut(s) 385, 849
GsaI CCCAGC 3 cut(s) 277, 611, 673
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 4 cut(s) 86, 470, 480, 629
HapII CCGG 6 cut(s) 73, 284, 360, 477, 813, 987
HgaI GACGC 2 cut(s) 319, 854
HhaI GCGC 2 cut(s) 386, 850
Hin1II CATG 2 cut(s) 469, 1135
Hin6I GCGC 2 cut(s) 384, 848
HinP1I GCGC 2 cut(s) 384, 848
HincII GTYRAC 2 cut(s) 614, 1128
HindII GTYRAC 2 cut(s) 614, 1128
HinfI GANTC 4 cut(s) 377, 798, 961, 1067
HpaII CCGG 6 cut(s) 73, 284, 360, 477, 813, 987
HphI GGTGA 8 cut(s) 100, 169, 280, 316, 418, 496, 715, 893
Hpy166II GTNNAC 4 cut(s) 614, 723, 1128, 1141
Hpy188I TCNGA 7 cut(s) 335, 463, 496, 550, 559, 570, 744
Hpy188III TCNNGA 6 cut(s) 52, 157, 264, 789, 802, 973
Hpy8I GTNNAC 4 cut(s) 614, 723, 1128, 1141
Hpy99I CGWCG 7 cut(s) 155, 158, 335, 347, 374, 377, 623
HpyAV CCTTC 9 cut(s) 100, 121, 151, 193, 210, 322, 496, 970, 1078
HpyCH4III ACNGT 2 cut(s) 151, 730
HpyCH4IV ACGT 2 cut(s) 621, 695
HpyCH4V TGCA 3 cut(s) 583, 662, 1175
HpyF10VI GCNNNNNNNGC 7 cut(s) 34, 73, 306, 856, 1046, 1055, 1181
HpyF3I CTNAG 2 cut(s) 50, 855
HpySE526I ACGT 2 cut(s) 621, 695
Hsp92II CATG 2 cut(s) 469, 1135
HspAI GCGC 2 cut(s) 384, 848
KflI GGGWCCC 1 cut(s) 165
KspI CCGCGG 1 cut(s) 38
Kzo9I GATC 5 cut(s) 7, 266, 565, 739, 1103
LguI GCTCTTC 1 cut(s) 920
LmnI GCTCC 6 cut(s) 45, 54, 72, 479, 604, 1223
Lsp1109I GCAGC 3 cut(s) 76, 646, 1187
LweI GCATC 3 cut(s) 924, 1036, 1186
MaeI CTAG 6 cut(s) 453, 680, 789, 863, 1071, 1200
MaeII ACGT 2 cut(s) 621, 695
MaeIII GTNAC 2 cut(s) 286, 691
MalI GATC 5 cut(s) 9, 268, 567, 741, 1105
MbiI CCGCTC 1 cut(s) 474
MboI GATC 5 cut(s) 7, 266, 565, 739, 1103
MhlI GDGCHC 2 cut(s) 59, 1228
MluCI AATT 7 cut(s) 434, 500, 544, 553, 584, 757, 1218
MlyI GAGTC 2 cut(s) 371, 807
MmeI TCCRAC 4 cut(s) 123, 313, 486, 519
MseI TTAA 6 cut(s) 444, 735, 756, 909, 926, 1095
MslI CAYNNNNRTG 1 cut(s) 824
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 6 cut(s) 73, 284, 360, 477, 813, 987
MspR9I CCNGG 2 cut(s) 958, 988
MvaI CCWGG 1 cut(s) 958
MvnI CGCG 6 cut(s) 37, 195, 330, 384, 618, 848
MwoI GCNNNNNNNGC 7 cut(s) 34, 73, 306, 856, 1046, 1055, 1181
NciI CCSGG 1 cut(s) 988
NcoI CCATGG 1 cut(s) 1131
NdeI CATATG 1 cut(s) 664
NdeII GATC 5 cut(s) 7, 266, 565, 739, 1103
NheI GCTAGC 1 cut(s) 1199
NlaIII CATG 2 cut(s) 469, 1135
NlaIV GGNNCC 4 cut(s) 41, 166, 167, 337
NmeAIII GCCGAG 2 cut(s) 62, 690
NmuCI GTSAC 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 920
PcsI WCGNNNNNNNCGW 2 cut(s) 339, 1025
PfeI GAWTC 2 cut(s) 961, 1067
PleI GAGTC 2 cut(s) 371, 806
PpsI GAGTC 2 cut(s) 371, 806
Ppu21I YACGTR 1 cut(s) 696
PpuMI RGGWCCY 1 cut(s) 165
Psp5II RGGWCCY 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 956
PspEI GGTNACC 1 cut(s) 286
PspFI CCCAGC 3 cut(s) 273, 607, 669
PspGI CCWGG 1 cut(s) 956
PspN4I GGNNCC 4 cut(s) 41, 166, 167, 337
PspPI GGNCC 3 cut(s) 165, 469, 628
PspPPI RGGWCCY 1 cut(s) 165
RsaI GTAC 1 cut(s) 899
RsaNI GTAC 1 cut(s) 898
RseI CAYNNNNRTG 1 cut(s) 824
SacII CCGCGG 1 cut(s) 38
SalI GTCGAC 1 cut(s) 1126
SapI GCTCTTC 1 cut(s) 920
SaqAI TTAA 6 cut(s) 444, 735, 756, 909, 926, 1095
Sau3AI GATC 5 cut(s) 7, 266, 565, 739, 1103
Sau96I GGNCC 3 cut(s) 165, 469, 628
ScaI AGTACT 1 cut(s) 899
SchI GAGTC 2 cut(s) 371, 807
ScrFI CCNGG 2 cut(s) 958, 988
SduI GDGCHC 2 cut(s) 59, 1228
SfaNI GCATC 3 cut(s) 924, 1036, 1186
Sfr303I CCGCGG 1 cut(s) 38
SgrBI CCGCGG 1 cut(s) 38
SinI GGWCC 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 824
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
SnaBI TACGTA 1 cut(s) 696
Sse9I AATT 7 cut(s) 434, 500, 544, 553, 584, 757, 1218
SspMI CTAG 6 cut(s) 453, 680, 789, 863, 1071, 1200
StyD4I CCNGG 2 cut(s) 956, 986
StyI CCWWGG 2 cut(s) 1113, 1131
TaaI ACNGT 2 cut(s) 151, 730
TaiI ACGT 2 cut(s) 624, 698
TaqI TCGA 6 cut(s) 156, 189, 342, 972, 1065, 1127
TasI AATT 7 cut(s) 434, 500, 544, 553, 584, 757, 1218
TatI WGTACW 1 cut(s) 897
TauI GCSGC 7 cut(s) 40, 64, 239, 300, 303, 543, 629
TfiI GAWTC 2 cut(s) 961, 1067
Tru1I TTAA 6 cut(s) 444, 735, 756, 909, 926, 1095
Tru9I TTAA 6 cut(s) 444, 735, 756, 909, 926, 1095
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 3 cut(s) 64, 659, 1175
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 6 cut(s) 175, 400, 580, 808, 907, 1220
VpaK11BI GGWCC 1 cut(s) 165
XagI CCTNNNNNAGG 1 cut(s) 344
XapI RAATTY 1 cut(s) 553
XbaI TCTAGA 1 cut(s) 788
XmiI GTMKAC 2 cut(s) 1127, 1140
XspI CTAG 6 cut(s) 453, 680, 789, 863, 1071, 1200
ZrmI AGTACT 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.