Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002522

Physical Location & Seq

Genomic Coordinates Reverse (-)

17875527 .. 17878383

Gene Structure 2857 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1096800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGGCTTGCATGAAGCTTGGAGCTAAGTCTGAAGCATTCCATCGCGAAGGCCAAACTTGGCTTTGCACAACCGGGCTTCCGAGCGATGTTGTGATTCAAGTTGGGGAAATGTCTTTTCACCTCCACAAGTTTCCGTTGCTTTCTAAAAGTGGGCTACTAGAGAGGCTTATCGAAGAGTGTTCTAGTGAGGAAGGATCTTCTTGTGCCTTGCAACTTCATGGTGTGCCCGGGGGAGCTAAAGCATTTGAGCTTGTAACCAAATTCTGCTATGGCGTCAAAATAGAACTGACTGCTATGAACGTAGTCATCCTTAGATGTGCAGCAGAATACCTCCAAATGACTGAAGATTACGAACAAGGAAATCTCATTGCGCAAACGGAGGTTTTCCTTACTGAAGTCTTCAGCAATTGGAGAGACTCCATAGTAGCTCTTGAAACATGTGAAGAAGTTCAACAATATGCTGAAGAGCTCCACATTGCTTCACGATGCATTGATTCCTTGGCTATCAAAGCTTGTGCAGATCCAAAGTTATTCAGTTGGCCTATATTATCAGGAGAAAATGACATGAAGAACCCAACAGCCTCATGGAATGGAATAACTTCTGATGACGCTAAGCCCCAACCTCTCGCTGATGATTGGTGGTATGAAGATGCATCCTTACTTAGCTTACCTCTCTATAAGCGATTGATTCAAGCCGTTGAATTGAAAGGAATGATGCCTGAGAGTATTGCCGCTTCTCTCATATTCTATGTTAGAAAGTATCTCCCCTTGATGAATAGACAGTCGAGCTTTCATGACACAAATTATGTAAACTCAGGCACCACCATTTCTAACATTTCTGAGGCAGACCAGAGGGCTCTACTTGAAGATATCATGGGGTTACTTCCTCATAAGAAAGGAGTCACATCCTCCAAGATTCTCCTTAGGCTGCTACGAACGGCTATGATCTTACATGCAAGTCCGTCATGCAGAGAAAATTTGGAGAAAAGGGTAGGTGCCCAGTTGGACCAAGCTGTGCTCATAGATCTTCTCATACCAAATATGGGCTATTCTGTGGAGACTCTTTACGACATAGACTGCATTCAGAGGATTCTTGATCATTTCATGTCAGTAAACCAGGCTTCACTTGCATCATCTTCCCCATGCATAATCGAAGAGGGGCAACTAATGACTGGCACTGATCCACTGACACCTATTACTATGGTAGCCAGTCTGGTGGATGGATACCTTGCCGAGGTAGCACCAGATGTTAATTTGAAGTTACCAAAATTTCAGGCACTTGCTGCTGTCATCCCTGAATATGCCCGGCCGGTGGATGATGGCATATATCATGCAATCGATGTTTTCTTGAAGGCACATCCTTGGCTTACAGATGCTGAAAGGGAGCAACTCTGCAGACTCATGAACTGCCAGAAGCTTTCTCTGGAAGCCAGTACGCATGCAGCTCAGAACGAAAGATTACCTCTTCGAGTAATTGTTCAAGTCCTTTTCTTTGAACAGCTTCGACTCCGCACATCAATTTCTGGCTGGTTCTTTGTTGACAATCTTGAGAACTCACAAAATCCCAGCGGAAACCTTGGGCTCCCCGCCAAAAATGATGTGTCCGGTCAAGTGGACGCTGCACAAGACCAAGCTGTGGGTTTCGATCATCATGACATGAGGGAACGTGTTTTAGAGCTTCAAAAGGAGTGTTCAAGCATGAAGAAGGAGCTCCAGAAGCTGGTCAAGGCCAAGAGAAGTTGGAGCATCCTTCCCATAAGGTTAGGTTTTAGAAAAAAGTTGGAGCCTTGCAATCCGAAAGAATCGAAGCCCACTGATCGGATTCCGGTACCAACATCATGCTGCAATGGACAACAAACTCATGAAAACAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB NPH3

Protein Analysis

625

Amino Acids

69.66

Weight (kDa)

5.34

Isoelectric Point (pI)

43.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	26 - 115	2.4e-08	BTB/POZ domain
NPH3	PF03000	212 - 478	7.2e-93	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03250 AT5G03250
fragaria_vesca	FvH4_6g16010 FvH4_7g19310 FvH4_7g19310 FvH4_7g19310
malus_domestica	MD01G1107700.v1.1 MD04G1096800.v1.1 MD07G1173300.v1.1
prunus_persica	Prupe.2G212500_v2.0.a1 Prupe.6G236100_v2.0.a1
pyrus_communis	pycom01g13460 pycom04g09190 pycom07g16800 pycom12g11690
rosa_chinensis	RchiOBHm_Chr1g0363191 RchiOBHm_Chr3g0469731
rosa_laevigata	RLG00000024326 RLG00000027585
rosa_multiflora	Rmu_sc0002925.1_g000004 Rmu_sc0010644.1_g000001 Rmu_sc0022819.1_g000002
rosa_roxburghii	Rroxscaffold_4G00293390 Rroxscaffold_6G00411520
rosa_rugosa	Rorug01G0305300 Rorug03G0103400 Rorug03G0103500 Rorug03G0103600 Rorug03G0103700 Rorug03G0103800 Rorug03G0103800
rosa_samantha	Rh1AG314100 Rh1BG277600 Rh1CG294000 Rh1DG308300 Rh3AG153200 Rh3BG177200 Rh3CG168100 Rh3DG198400
rosa_wichuraiana	Rw1G027830 Rw3G014440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	372
Acc65I	GGTACC	1 cut(s)	1828
AccB1I	GGYRCC	3 cut(s)	818, 995, 1828
AccB7I	CCANNNNNTGG	2 cut(s)	1636, 1720
AccII	CGCG	1 cut(s)	45
AciI	CCGC	4 cut(s)	732, 1510, 1569, 1587
AclWI	GGATC	3 cut(s)	202, 515, 1175
AcoI	YGGCCR	1 cut(s)	1307
AcsI	RAATTY	3 cut(s)	260, 976, 1268
AcuI	CTGAAG	5 cut(s)	51, 363, 385, 414, 483
AcyI	GRCGYC	1 cut(s)	273
AfaI	GTAC	2 cut(s)	1435, 1830
AfiI	CCNNNNNNNGG	6 cut(s)	1043, 1234, 1312, 1636, 1720, 1818
AflIII	ACRYGT	2 cut(s)	437, 1666
AjnI	CCWGG	1 cut(s)	1116
Alw21I	GWGCWC	3 cut(s)	471, 1020, 1713
Alw26I	GTCTC	2 cut(s)	408, 1052
AlwI	GGATC	3 cut(s)	202, 515, 1175
AlwNI	CAGNNNCTG	2 cut(s)	1376, 1720
Ama87I	CYCGRG	1 cut(s)	227
AoxI	GGCC	4 cut(s)	49, 539, 1307, 1728
ApeKI	GCWGC	6 cut(s)	320, 928, 1283, 1442, 1619, 1842
ApoI	RAATTY	3 cut(s)	260, 976, 1268
Asp700I	GAANNNNTTC	3 cut(s)	447, 598, 1500
Asp718I	GGTACC	1 cut(s)	1828
AspLEI	GCGC	1 cut(s)	373
AspS9I	GGNCC	1 cut(s)	1006
AsuC2I	CCSGG	4 cut(s)	73, 228, 229, 1306
AsuHPI	GGTGA	1 cut(s)	110
AvaI	CYCGRG	1 cut(s)	227
AvaII	GGWCC	1 cut(s)	1006
AxyI	CCTNAGG	1 cut(s)	923
BaeGI	GKGCMC	2 cut(s)	228, 1000
BanI	GGYRCC	3 cut(s)	818, 995, 1828
BanII	GRGCYC	4 cut(s)	471, 859, 1584, 1713
BarI	GAAGNNNNNNTAC	2 cut(s)	718, 750
BbsI	GAAGAC	1 cut(s)	391
Bbv12I	GWGCWC	3 cut(s)	471, 1020, 1713
BbvI	GCAGC	6 cut(s)	332, 915, 1270, 1454, 1606, 1829
BccI	CCATC	3 cut(s)	48, 1214, 1313
BceAI	ACGGC	2 cut(s)	680, 954
BciT130I	CCWGG	1 cut(s)	1118
BciVI	GTATCC	1 cut(s)	1217
BclI	TGATCA	1 cut(s)	1096
BcnI	CCSGG	4 cut(s)	73, 228, 229, 1306
BcoDI	GTCTC	2 cut(s)	408, 1052
BfaI	CTAG	3 cut(s)	158, 183, 1873
BfmI	CTRYAG	1 cut(s)	1393
BfuI	GTATCC	1 cut(s)	1217
BglII	AGATCT	1 cut(s)	1024
BisI	GCNGC	7 cut(s)	321, 732, 929, 1284, 1443, 1620, 1843
BlpI	GCTNAGC	1 cut(s)	612
BlsI	GCNGC	7 cut(s)	322, 733, 930, 1285, 1444, 1621, 1844
Bme1390I	CCNGG	5 cut(s)	73, 228, 229, 1118, 1306
Bme18I	GGWCC	1 cut(s)	1006
BmeT110I	CYCGRG	1 cut(s)	227
BmgT120I	GGNCC	1 cut(s)	1006
BmiI	GGNNCC	5 cut(s)	820, 997, 1583, 1785, 1830
BmrFI	CCNGG	5 cut(s)	73, 228, 229, 1118, 1306
BmrI	ACTGGG	1 cut(s)	994
BmsI	GCATC	7 cut(s)	476, 640, 662, 705, 1139, 1363, 1755
BmuI	ACTGGG	1 cut(s)	994
BpiI	GAAGAC	1 cut(s)	391
BpmI	CTGGAG	1 cut(s)	1697
Bpu1102I	GCTNAGC	1 cut(s)	612
BpuEI	CTTGAG	1 cut(s)	1568
BpuMI	CCSGG	4 cut(s)	73, 228, 229, 1306
Bsa29I	ATCGAT	1 cut(s)	1338
BsaHI	GRCGYC	1 cut(s)	273
BsaJI	CCNNGG	6 cut(s)	227, 228, 498, 1233, 1361, 1576
BsaWI	WCCGGW	2 cut(s)	1604, 1825
Bsc4I	CCNNNNNNNGG	6 cut(s)	1043, 1234, 1312, 1636, 1720, 1818
Bse118I	RCCGGY	1 cut(s)	1309
Bse1I	ACTGG	4 cut(s)	1000, 1177, 1209, 1431
Bse21I	CCTNAGG	1 cut(s)	923
Bse3DI	GCAATG	3 cut(s)	366, 474, 1852
BseBI	CCWGG	1 cut(s)	1118
BseCI	ATCGAT	1 cut(s)	1338
BseDI	CCNNGG	6 cut(s)	227, 228, 498, 1233, 1361, 1576
BseGI	GGATG	8 cut(s)	306, 653, 905, 1225, 1290, 1321, 1357, 1746
BseLI	CCNNNNNNNGG	6 cut(s)	1043, 1234, 1312, 1636, 1720, 1818
BseMI	GCAATG	3 cut(s)	366, 474, 1852
BseMII	CTCAG	4 cut(s)	711, 828, 831, 1460
BseNI	ACTGG	4 cut(s)	1000, 1177, 1209, 1431
BseSI	GKGCMC	2 cut(s)	228, 1000
BseX3I	CGGCCG	1 cut(s)	1307
BseXI	GCAGC	6 cut(s)	332, 915, 1270, 1454, 1606, 1829
BseYI	CCCAGC	1 cut(s)	1565
BsgI	GTGCAG	3 cut(s)	339, 537, 1605
Bsh1236I	CGCG	1 cut(s)	45
Bsh1285I	CGRYCG	1 cut(s)	1310
BshFI	GGCC	4 cut(s)	51, 541, 1309, 1730
BshNI	GGYRCC	3 cut(s)	818, 995, 1828
BshVI	ATCGAT	1 cut(s)	1338
BsiEI	CGRYCG	1 cut(s)	1310
BsiHKAI	GWGCWC	3 cut(s)	471, 1020, 1713
BsiHKCI	CYCGRG	1 cut(s)	227
BsiSI	CCGG	6 cut(s)	72, 228, 1306, 1310, 1605, 1826
BslI	CCNNNNNNNGG	6 cut(s)	1043, 1234, 1312, 1636, 1720, 1818
BsmAI	GTCTC	2 cut(s)	408, 1052
BsmI	GAATGC	2 cut(s)	35, 1080
BsnI	GGCC	4 cut(s)	51, 541, 1309, 1730
BsoBI	CYCGRG	1 cut(s)	227
Bsp1286I	GDGCHC	7 cut(s)	228, 471, 859, 1000, 1020, 1584, 1713
Bsp143I	GATC	8 cut(s)	194, 520, 945, 1024, 1096, 1180, 1645, 1816
Bsp1720I	GCTNAGC	1 cut(s)	612
Bsp68I	TCGCGA	1 cut(s)	45
BspACI	CCGC	4 cut(s)	732, 1510, 1569, 1587
BspANI	GGCC	4 cut(s)	51, 541, 1309, 1730
BspCNI	CTCAG	4 cut(s)	712, 827, 832, 1459
BspDI	ATCGAT	1 cut(s)	1338
BspFNI	CGCG	1 cut(s)	45
BspHI	TCATGA	4 cut(s)	793, 1401, 1651, 1861
BspLI	GGNNCC	5 cut(s)	820, 997, 1583, 1785, 1830
BspMAI	CTGCAG	1 cut(s)	1397
BspPI	GGATC	3 cut(s)	202, 515, 1175
BspQI	GCTCTTC	1 cut(s)	459
BspT107I	GGYRCC	3 cut(s)	818, 995, 1828
BsrDI	GCAATG	3 cut(s)	366, 474, 1852
BsrFI	RCCGGY	1 cut(s)	1309
BsrI	ACTGG	4 cut(s)	1000, 1177, 1209, 1431
BssAI	RCCGGY	1 cut(s)	1309
BssECI	CCNNGG	6 cut(s)	227, 228, 498, 1233, 1361, 1576
BssMI	GATC	8 cut(s)	194, 520, 945, 1024, 1096, 1180, 1645, 1816
BssNI	GRCGYC	1 cut(s)	273
BssT1I	CCWWGG	3 cut(s)	498, 1361, 1576
Bst2UI	CCWGG	1 cut(s)	1118
Bst4CI	ACNGT	1 cut(s)	783
Bst6I	CTCTTC	4 cut(s)	168, 459, 1149, 1470
BstACI	GRCGYC	1 cut(s)	273
BstAPI	GCANNNNNTGC	1 cut(s)	1283
BstC8I	GCNNGC	2 cut(s)	7, 1440
BstDEI	CTNAG	9 cut(s)	24, 311, 612, 662, 720, 814, 840, 923, 1446
BstENI	CCTNNNNNAGG	1 cut(s)	1232
BstF5I	GGATG	8 cut(s)	306, 653, 905, 1225, 1290, 1321, 1357, 1746
BstFNI	CGCG	1 cut(s)	45
BstHHI	GCGC	1 cut(s)	373
BstKTI	GATC	8 cut(s)	197, 523, 948, 1027, 1099, 1183, 1648, 1819
BstMAI	GTCTC	2 cut(s)	408, 1052
BstMBI	GATC	8 cut(s)	194, 520, 945, 1024, 1096, 1180, 1645, 1816
BstMCI	CGRYCG	1 cut(s)	1310
BstMWI	GCNNNNNNNGC	5 cut(s)	509, 1127, 1238, 1283, 1717
BstNI	CCWGG	1 cut(s)	1118
BstNSI	RCATGY	3 cut(s)	441, 956, 1442
BstSCI	CCNGG	5 cut(s)	71, 226, 227, 1116, 1304
BstSFI	CTRYAG	1 cut(s)	1393
BstSLI	GKGCMC	2 cut(s)	228, 1000
BstUI	CGCG	1 cut(s)	45
BstV1I	GCAGC	6 cut(s)	332, 915, 1270, 1454, 1606, 1829
BstV2I	GAAGAC	1 cut(s)	391
BstX2I	RGATCY	3 cut(s)	194, 520, 1024
BstXI	CCANNNNNNTGG	1 cut(s)	1216
BstYI	RGATCY	3 cut(s)	194, 520, 1024
BstZI	CGGCCG	1 cut(s)	1307
Bsu15I	ATCGAT	1 cut(s)	1338
Bsu36I	CCTNAGG	1 cut(s)	923
BsuI	GTATCC	1 cut(s)	1217
BsuRI	GGCC	4 cut(s)	51, 541, 1309, 1730
BsuTUI	ATCGAT	1 cut(s)	1338
BtgZI	GCGATG	2 cut(s)	26, 99
BtsCI	GGATG	8 cut(s)	306, 653, 905, 1225, 1290, 1321, 1357, 1746
BtsIMutI	CAGTG	3 cut(s)	1176, 1184, 1812
BtuMI	TCGCGA	1 cut(s)	45
Cac8I	GCNNGC	2 cut(s)	7, 1440
CaiI	CAGNNNCTG	2 cut(s)	1376, 1720
CciI	TCATGA	4 cut(s)	793, 1401, 1651, 1861
CfoI	GCGC	1 cut(s)	373
Cfr10I	RCCGGY	1 cut(s)	1309
Cfr13I	GGNCC	1 cut(s)	1006
Cfr9I	CCCGGG	1 cut(s)	227
ClaI	ATCGAT	1 cut(s)	1338
CseI	GACGC	3 cut(s)	262, 617, 1625
Csp6I	GTAC	2 cut(s)	1434, 1829
CviQI	GTAC	2 cut(s)	1434, 1829
DdeI	CTNAG	9 cut(s)	24, 311, 612, 662, 720, 814, 840, 923, 1446
DpnI	GATC	8 cut(s)	196, 522, 947, 1026, 1098, 1182, 1647, 1818
DpnII	GATC	8 cut(s)	194, 520, 945, 1024, 1096, 1180, 1645, 1816
EaeI	YGGCCR	1 cut(s)	1307
EagI	CGGCCG	1 cut(s)	1307
Eam1104I	CTCTTC	4 cut(s)	168, 459, 1149, 1470
EarI	CTCTTC	4 cut(s)	168, 459, 1149, 1470
Ecl136II	GAGCTC	2 cut(s)	469, 1711
EclXI	CGGCCG	1 cut(s)	1307
Eco130I	CCWWGG	3 cut(s)	498, 1361, 1576
Eco24I	GRGCYC	4 cut(s)	471, 859, 1584, 1713
Eco32I	GATATC	1 cut(s)	871
Eco47I	GGWCC	1 cut(s)	1006
Eco52I	CGGCCG	1 cut(s)	1307
Eco53kI	GAGCTC	2 cut(s)	469, 1711
Eco57I	CTGAAG	5 cut(s)	51, 363, 385, 414, 483
Eco81I	CCTNAGG	1 cut(s)	923
Eco88I	CYCGRG	1 cut(s)	227
EcoICRI	GAGCTC	2 cut(s)	469, 1711
EcoNI	CCTNNNNNAGG	1 cut(s)	1232
EcoRII	CCWGG	1 cut(s)	1116
EcoRV	GATATC	1 cut(s)	871
EcoT14I	CCWWGG	3 cut(s)	498, 1361, 1576
EcoT22I	ATGCAT	3 cut(s)	491, 655, 1148
EcoT38I	GRGCYC	4 cut(s)	471, 859, 1584, 1713
ErhI	CCWWGG	3 cut(s)	498, 1361, 1576
FauI	CCCGC	1 cut(s)	1594
FbaI	TGATCA	1 cut(s)	1096
Fnu4HI	GCNGC	7 cut(s)	321, 732, 929, 1284, 1443, 1620, 1843
FokI	GGATG	8 cut(s)	293, 640, 892, 1232, 1277, 1328, 1344, 1733
FriOI	GRGCYC	4 cut(s)	471, 859, 1584, 1713
Fsp4HI	GCNGC	7 cut(s)	321, 732, 929, 1284, 1443, 1620, 1843
FspBI	CTAG	3 cut(s)	158, 183, 1873
FspI	TGCGCA	1 cut(s)	372
GlaI	GCGC	1 cut(s)	372
GluI	GCNGC	7 cut(s)	321, 732, 929, 1284, 1443, 1620, 1843
GsaI	CCCAGC	1 cut(s)	1569
GsuI	CTGGAG	1 cut(s)	1697
HaeIII	GGCC	4 cut(s)	51, 541, 1309, 1730
HapII	CCGG	6 cut(s)	72, 228, 1306, 1310, 1605, 1826
HgaI	GACGC	3 cut(s)	262, 617, 1625
HhaI	GCGC	1 cut(s)	373
Hin1I	GRCGYC	1 cut(s)	273
Hin6I	GCGC	1 cut(s)	371
HinP1I	GCGC	1 cut(s)	371
HincII	GTYRAC	1 cut(s)	1540
HindII	GTYRAC	1 cut(s)	1540
HindIII	AAGCTT	3 cut(s)	14, 510, 1415
HpaII	CCGG	6 cut(s)	72, 228, 1306, 1310, 1605, 1826
HphI	GGTGA	1 cut(s)	110
Hpy166II	GTNNAC	4 cut(s)	811, 1114, 1540, 1615
Hpy188I	TCNGA	8 cut(s)	31, 81, 604, 841, 1086, 1449, 1797, 1821
Hpy8I	GTNNAC	4 cut(s)	811, 1114, 1540, 1615
HpyAV	CCTTC	5 cut(s)	41, 185, 1345, 1699, 1760
HpyCH4III	ACNGT	1 cut(s)	783
HpyCH4IV	ACGT	2 cut(s)	300, 1666
HpyF10VI	GCNNNNNNNGC	5 cut(s)	509, 1127, 1238, 1283, 1717
HpyF3I	CTNAG	9 cut(s)	24, 311, 612, 662, 720, 814, 840, 923, 1446
HpySE526I	ACGT	2 cut(s)	300, 1666
Hsp92I	GRCGYC	1 cut(s)	273
HspAI	GCGC	1 cut(s)	371
KpnI	GGTACC	1 cut(s)	1832
Ksp22I	TGATCA	1 cut(s)	1096
Kzo9I	GATC	8 cut(s)	194, 520, 945, 1024, 1096, 1180, 1645, 1816
LguI	GCTCTTC	1 cut(s)	459
LmnI	GCTCC	9 cut(s)	20, 233, 474, 1384, 1587, 1708, 1716, 1743, 1783
Lsp1109I	GCAGC	6 cut(s)	332, 915, 1270, 1454, 1606, 1829
LweI	GCATC	7 cut(s)	476, 640, 662, 705, 1139, 1363, 1755
MaeI	CTAG	3 cut(s)	158, 183, 1873
MaeII	ACGT	2 cut(s)	300, 1666
MaeIII	GTNAC	4 cut(s)	253, 879, 901, 1260
MalI	GATC	8 cut(s)	196, 522, 947, 1026, 1098, 1182, 1647, 1818
MboI	GATC	8 cut(s)	194, 520, 945, 1024, 1096, 1180, 1645, 1816
MfeI	CAATTG	1 cut(s)	406
MflI	RGATCY	3 cut(s)	194, 520, 1024
MhlI	GDGCHC	7 cut(s)	228, 471, 859, 1000, 1020, 1584, 1713
MluCI	AATT	9 cut(s)	260, 406, 701, 802, 976, 1252, 1268, 1473, 1518
MlyI	GAGTC	5 cut(s)	410, 909, 1054, 1392, 1500
MmeI	TCCRAC	3 cut(s)	984, 1721, 1761
Mph1103I	ATGCAT	3 cut(s)	491, 655, 1148
MroXI	GAANNNNTTC	3 cut(s)	447, 598, 1500
MseI	TTAA	1 cut(s)	1251
MspA1I	CMGCKG	1 cut(s)	1569
MspI	CCGG	6 cut(s)	72, 228, 1306, 1310, 1605, 1826
MspR9I	CCNGG	5 cut(s)	73, 228, 229, 1118, 1306
MunI	CAATTG	1 cut(s)	406
Mva1269I	GAATGC	2 cut(s)	35, 1080
MvaI	CCWGG	1 cut(s)	1118
MvnI	CGCG	1 cut(s)	45
MwoI	GCNNNNNNNGC	5 cut(s)	509, 1127, 1238, 1283, 1717
NciI	CCSGG	4 cut(s)	73, 228, 229, 1306
NdeII	GATC	8 cut(s)	194, 520, 945, 1024, 1096, 1180, 1645, 1816
NlaIV	GGNNCC	5 cut(s)	820, 997, 1583, 1785, 1830
NmeAIII	GCCGAG	1 cut(s)	1258
NmuCI	GTSAC	1 cut(s)	901
NruI	TCGCGA	1 cut(s)	45
NsbI	TGCGCA	1 cut(s)	372
NsiI	ATGCAT	3 cut(s)	491, 655, 1148
NspI	RCATGY	3 cut(s)	441, 956, 1442
PaeI	GCATGC	1 cut(s)	1442
PagI	TCATGA	4 cut(s)	793, 1401, 1651, 1861
PciI	ACATGT	1 cut(s)	437
PciSI	GCTCTTC	1 cut(s)	459
PctI	GAATGC	2 cut(s)	35, 1080
PdmI	GAANNNNTTC	3 cut(s)	447, 598, 1500
PfeI	GAWTC	7 cut(s)	94, 494, 688, 916, 1090, 1802, 1822
PflMI	CCANNNNNTGG	2 cut(s)	1636, 1720
PkrI	GCNGC	7 cut(s)	322, 733, 930, 1285, 1444, 1621, 1844
PleI	GAGTC	5 cut(s)	410, 908, 1054, 1392, 1500
PpsI	GAGTC	5 cut(s)	410, 908, 1054, 1392, 1500
PscI	ACATGT	1 cut(s)	437
Psp124BI	GAGCTC	2 cut(s)	471, 1713
Psp6I	CCWGG	1 cut(s)	1116
PspFI	CCCAGC	1 cut(s)	1565
PspGI	CCWGG	1 cut(s)	1116
PspN4I	GGNNCC	5 cut(s)	820, 997, 1583, 1785, 1830
PspPI	GGNCC	1 cut(s)	1006
PstI	CTGCAG	1 cut(s)	1397
PstNI	CAGNNNCTG	2 cut(s)	1376, 1720
PsuI	RGATCY	3 cut(s)	194, 520, 1024
RruI	TCGCGA	1 cut(s)	45
RsaI	GTAC	2 cut(s)	1435, 1830
RsaNI	GTAC	2 cut(s)	1434, 1829
SacI	GAGCTC	2 cut(s)	471, 1713
SapI	GCTCTTC	1 cut(s)	459
SaqAI	TTAA	1 cut(s)	1251
SatI	GCNGC	7 cut(s)	321, 732, 929, 1284, 1443, 1620, 1843
Sau3AI	GATC	8 cut(s)	194, 520, 945, 1024, 1096, 1180, 1645, 1816
Sau96I	GGNCC	1 cut(s)	1006
SchI	GAGTC	5 cut(s)	410, 909, 1054, 1392, 1500
ScrFI	CCNGG	5 cut(s)	73, 228, 229, 1118, 1306
SduI	GDGCHC	7 cut(s)	228, 471, 859, 1000, 1020, 1584, 1713
SfaNI	GCATC	7 cut(s)	476, 640, 662, 705, 1139, 1363, 1755
SfcI	CTRYAG	1 cut(s)	1393
SinI	GGWCC	1 cut(s)	1006
SmaI	CCCGGG	1 cut(s)	229
SmlI	CTYRAG	1 cut(s)	1547
SmoI	CTYRAG	1 cut(s)	1547
SphI	GCATGC	1 cut(s)	1442
Sse9I	AATT	9 cut(s)	260, 406, 701, 802, 976, 1252, 1268, 1473, 1518
SsiI	CCGC	4 cut(s)	732, 1510, 1569, 1587
SspMI	CTAG	3 cut(s)	158, 183, 1873
SstI	GAGCTC	2 cut(s)	471, 1713
StyD4I	CCNGG	5 cut(s)	71, 226, 227, 1116, 1304
StyI	CCWWGG	3 cut(s)	498, 1361, 1576
TaaI	ACNGT	1 cut(s)	783
TaiI	ACGT	2 cut(s)	303, 1669
TaqI	TCGA	8 cut(s)	171, 785, 1152, 1338, 1468, 1504, 1644, 1805
TasI	AATT	9 cut(s)	260, 406, 701, 802, 976, 1252, 1268, 1473, 1518
TauI	GCSGC	1 cut(s)	734
TfiI	GAWTC	7 cut(s)	94, 494, 688, 916, 1090, 1802, 1822
Tru1I	TTAA	1 cut(s)	1251
Tru9I	TTAA	1 cut(s)	1251
TscAI	CASTG	3 cut(s)	1183, 1191, 1819
TseFI	GTSAC	1 cut(s)	901
TseI	GCWGC	6 cut(s)	320, 928, 1283, 1442, 1619, 1842
Tsp45I	GTSAC	1 cut(s)	901
TspGWI	ACGGA	3 cut(s)	123, 392, 951
TspMI	CCCGGG	1 cut(s)	227
TspRI	CASTG	3 cut(s)	1183, 1191, 1819
Van91I	CCANNNNNTGG	2 cut(s)	1636, 1720
VpaK11BI	GGWCC	1 cut(s)	1006
XagI	CCTNNNNNAGG	1 cut(s)	1232
XapI	RAATTY	3 cut(s)	260, 976, 1268
XceI	RCATGY	3 cut(s)	441, 956, 1442
XcmI	CCANNNNNNNNNTGG	1 cut(s)	582
XmaI	CCCGGG	1 cut(s)	227
XmnI	GAANNNNTTC	3 cut(s)	447, 598, 1500
XspI	CTAG	3 cut(s)	158, 183, 1873
Zsp2I	ATGCAT	3 cut(s)	491, 655, 1148

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.